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Q-CROC Amplification ⚙
Reference Genes : primer pair list
Gene Forward Reverse
VIEW GAPDH/GAPD/NCAPD2andGAPDH GAPDH_forward1 GAPDH_reverse2
VIEW HMBS/HMBS HMBS_G_3_f HMBS_G_3_r
VIEW HPRT1/HPRT1 HPRT1_global_for_1 HPRT1_global_rev_1
VIEW MRPL19/MRPL19 MRPL19_G_3_f MRPL19_G_3_r
VIEW PSMC4/PSMC4 PSMC4_G_1_f PSMC4_G_1_r
VIEW PUM1 PUM1_global_for_1 PUM1_global_rev_1
VIEW SDHA/SDHA SDHA_G_f SDHA_G_r
VIEW SF3A1/SF3A1 SF3A1_global_for_1 SF3A1_global_rev_1
VIEW YWHAZ/YWHAZ YWHAZ_G_f YWHAZ_G_r
QPCR Relative Expression Quantification : NF pairwise rate map
QPCR Relative Expression Quantification : NF Histogram
QPCR Relative Expression Quantification : Reference Genes list

Reference Genes For Relative Expression Analysis

  • SDHA_G_f SDHA_G_r
  • HMBS_G_3_f HMBS_G_3_r
  • PSMC4_G_1_f PSMC4_G_1_r
QPCR Relative Expression Quantification : Reports

Comments on the experiments

  • Machine: Biorad Milou
  • Mmix: Biorad
QPCR Genorm : M -- average pairwise expression ratio
Primer Pair 9 8 7 6 5 4 3 2 1
YWHAZ_G_f YWHAZ_G_r 1.382
MRPL19_G_3_f MRPL19_G_3_r 1.129 0.991
HPRT1_global_for_1 HPRT1_global_rev_1 0.937 0.891 0.841
GAPDH_forward1 GAPDH_reverse2 0.752 0.731 0.643 0.682
PUM1_global_for_1 PUM1_global_rev_1 0.648 0.586 0.511 0.517 0.587
SF3A1_global_for_1 SF3A1_global_rev_1 0.629 0.551 0.489 0.473 0.500 0.624
SDHA_G_f SDHA_G_r 0.877 0.774 0.754 0.640 0.532 0.417 0.417
HMBS_G_3_f HMBS_G_3_r 0.779 0.664 0.653 0.552 0.450 0.353 0.353
PSMC4_G_1_f PSMC4_G_1_r 0.715 0.624 0.577 0.480 0.405 0.340 0.340

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