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Supplementary data
RBFOX2 enforces the mesenchymal splicing signature in normal and cancer tissues
7. 26 switch-like targets from epithelium and mesenchyme (fetal colon) in RBFOX2, MBNL1, PTBP and ESRP knockdowns
Transcript map
: MBNL1 Control (MDA-MB-231)
Exon
Length: 171
Transcript: AK307420
Length: 171
Transcript: AK307420
Exon
Length: 204
Transcript: AK307420
Length: 204
Transcript: AK307420
Exon
Length: 258
Transcript: AK307420
Length: 258
Transcript: AK307420
Exon
Length: 49
Transcript: AK307420
Length: 49
Transcript: AK307420
Exon
Length: 36
Transcript: AK307420
Length: 36
Transcript: AK307420
Exon
Length: 75
Transcript: AK307420
Length: 75
Transcript: AK307420
Exon
Length: 3396
Transcript: AK307420
Length: 3396
Transcript: AK307420
Intron
Length: 17605
Transcript: AK307420
Length: 17605
Transcript: AK307420
Intron
Length: 12361
Transcript: AK307420
Length: 12361
Transcript: AK307420
Intron
Length: 2185
Transcript: AK307420
Length: 2185
Transcript: AK307420
Intron
Length: 7768
Transcript: AK307420
Length: 7768
Transcript: AK307420
Intron
Length: 3693
Transcript: AK307420
Length: 3693
Transcript: AK307420
Intron
Length: 3039
Transcript: AK307420
Length: 3039
Transcript: AK307420
Exon
Length: 1053
Transcript: BC050535
Length: 1053
Transcript: BC050535
Exon
Length: 120
Transcript: BC050535
Length: 120
Transcript: BC050535
Exon
Length: 58
Transcript: BC050535
Length: 58
Transcript: BC050535
Exon
Length: 171
Transcript: BC050535
Length: 171
Transcript: BC050535
Exon
Length: 204
Transcript: BC050535
Length: 204
Transcript: BC050535
Exon
Length: 258
Transcript: BC050535
Length: 258
Transcript: BC050535
Exon
Length: 54
Transcript: BC050535
Length: 54
Transcript: BC050535
Exon
Length: 154
Transcript: BC050535
Length: 154
Transcript: BC050535
Exon
Length: 36
Transcript: BC050535
Length: 36
Transcript: BC050535
Exon
Length: 95
Transcript: BC050535
Length: 95
Transcript: BC050535
Exon
Length: 75
Transcript: BC050535
Length: 75
Transcript: BC050535
Exon
Length: 3396
Transcript: BC050535
Length: 3396
Transcript: BC050535
Intron
Length: 66018
Transcript: BC050535
Length: 66018
Transcript: BC050535
Intron
Length: 60936
Transcript: BC050535
Length: 60936
Transcript: BC050535
Intron
Length: 18716
Transcript: BC050535
Length: 18716
Transcript: BC050535
Intron
Length: 17605
Transcript: BC050535
Length: 17605
Transcript: BC050535
Intron
Length: 12361
Transcript: BC050535
Length: 12361
Transcript: BC050535
Intron
Length: 1164
Transcript: BC050535
Length: 1164
Transcript: BC050535
Intron
Length: 862
Transcript: BC050535
Length: 862
Transcript: BC050535
Intron
Length: 7768
Transcript: BC050535
Length: 7768
Transcript: BC050535
Intron
Length: 689
Transcript: BC050535
Length: 689
Transcript: BC050535
Intron
Length: 2909
Transcript: BC050535
Length: 2909
Transcript: BC050535
Intron
Length: 3039
Transcript: BC050535
Length: 3039
Transcript: BC050535
Reaction
Expected peak: 352
9.8M
refseq_MBNL1.F3
refseq_MBNL1.R3
Expected peak: 352
9.8M
refseq_MBNL1.F3
refseq_MBNL1.R3
Exon
Length: 1053
Transcript: NM_021038
Length: 1053
Transcript: NM_021038
Exon
Length: 963
Transcript: NM_021038
Length: 963
Transcript: NM_021038
Exon
Length: 171
Transcript: NM_021038
Length: 171
Transcript: NM_021038
Exon
Length: 204
Transcript: NM_021038
Length: 204
Transcript: NM_021038
Exon
Length: 258
Transcript: NM_021038
Length: 258
Transcript: NM_021038
Exon
Length: 154
Transcript: NM_021038
Length: 154
Transcript: NM_021038
Exon
Length: 36
Transcript: NM_021038
Length: 36
Transcript: NM_021038
Exon
Length: 95
Transcript: NM_021038
Length: 95
Transcript: NM_021038
Exon
Length: 75
Transcript: NM_021038
Length: 75
Transcript: NM_021038
Exon
Length: 3396
Transcript: NM_021038
Length: 3396
Transcript: NM_021038
Intron
Length: 30312
Transcript: NM_021038
Length: 30312
Transcript: NM_021038
Intron
Length: 114573
Transcript: NM_021038
Length: 114573
Transcript: NM_021038
Intron
Length: 17605
Transcript: NM_021038
Length: 17605
Transcript: NM_021038
Intron
Length: 12361
Transcript: NM_021038
Length: 12361
Transcript: NM_021038
Intron
Length: 2080
Transcript: NM_021038
Length: 2080
Transcript: NM_021038
Intron
Length: 7768
Transcript: NM_021038
Length: 7768
Transcript: NM_021038
Intron
Length: 689
Transcript: NM_021038
Length: 689
Transcript: NM_021038
Intron
Length: 2909
Transcript: NM_021038
Length: 2909
Transcript: NM_021038
Intron
Length: 3039
Transcript: NM_021038
Length: 3039
Transcript: NM_021038
Reaction
Expected peak: 298
83.3M
refseq_MBNL1.F3
refseq_MBNL1.R3
Expected peak: 298
83.3M
refseq_MBNL1.F3
refseq_MBNL1.R3
Exon
Length: 1053
Transcript: NM_207292
Length: 1053
Transcript: NM_207292
Exon
Length: 963
Transcript: NM_207292
Length: 963
Transcript: NM_207292
Exon
Length: 171
Transcript: NM_207292
Length: 171
Transcript: NM_207292
Exon
Length: 204
Transcript: NM_207292
Length: 204
Transcript: NM_207292
Exon
Length: 258
Transcript: NM_207292
Length: 258
Transcript: NM_207292
Exon
Length: 154
Transcript: NM_207292
Length: 154
Transcript: NM_207292
Exon
Length: 95
Transcript: NM_207292
Length: 95
Transcript: NM_207292
Exon
Length: 75
Transcript: NM_207292
Length: 75
Transcript: NM_207292
Exon
Length: 3396
Transcript: NM_207292
Length: 3396
Transcript: NM_207292
Intron
Length: 30312
Transcript: NM_207292
Length: 30312
Transcript: NM_207292
Intron
Length: 114573
Transcript: NM_207292
Length: 114573
Transcript: NM_207292
Intron
Length: 17605
Transcript: NM_207292
Length: 17605
Transcript: NM_207292
Intron
Length: 12361
Transcript: NM_207292
Length: 12361
Transcript: NM_207292
Intron
Length: 2080
Transcript: NM_207292
Length: 2080
Transcript: NM_207292
Intron
Length: 8493
Transcript: NM_207292
Length: 8493
Transcript: NM_207292
Intron
Length: 2909
Transcript: NM_207292
Length: 2909
Transcript: NM_207292
Intron
Length: 3039
Transcript: NM_207292
Length: 3039
Transcript: NM_207292
Reaction
Expected peak: 298
83.3M
refseq_MBNL1.F3
refseq_MBNL1.R3
Expected peak: 298
83.3M
refseq_MBNL1.F3
refseq_MBNL1.R3
Exon
Length: 963
Transcript: NM_207293
Length: 963
Transcript: NM_207293
Exon
Length: 171
Transcript: NM_207293
Length: 171
Transcript: NM_207293
Exon
Length: 204
Transcript: NM_207293
Length: 204
Transcript: NM_207293
Exon
Length: 258
Transcript: NM_207293
Length: 258
Transcript: NM_207293
Exon
Length: 54
Transcript: NM_207293
Length: 54
Transcript: NM_207293
Exon
Length: 154
Transcript: NM_207293
Length: 154
Transcript: NM_207293
Exon
Length: 95
Transcript: NM_207293
Length: 95
Transcript: NM_207293
Exon
Length: 75
Transcript: NM_207293
Length: 75
Transcript: NM_207293
Exon
Length: 3396
Transcript: NM_207293
Length: 3396
Transcript: NM_207293
Intron
Length: 114573
Transcript: NM_207293
Length: 114573
Transcript: NM_207293
Intron
Length: 17605
Transcript: NM_207293
Length: 17605
Transcript: NM_207293
Intron
Length: 12361
Transcript: NM_207293
Length: 12361
Transcript: NM_207293
Intron
Length: 1164
Transcript: NM_207293
Length: 1164
Transcript: NM_207293
Intron
Length: 862
Transcript: NM_207293
Length: 862
Transcript: NM_207293
Intron
Length: 8493
Transcript: NM_207293
Length: 8493
Transcript: NM_207293
Intron
Length: 2909
Transcript: NM_207293
Length: 2909
Transcript: NM_207293
Intron
Length: 3039
Transcript: NM_207293
Length: 3039
Transcript: NM_207293
Reaction
Expected peak: 352
9.8M
refseq_MBNL1.F3
refseq_MBNL1.R3
Expected peak: 352
9.8M
refseq_MBNL1.F3
refseq_MBNL1.R3
Exon
Length: 963
Transcript: NM_207294
Length: 963
Transcript: NM_207294
Exon
Length: 171
Transcript: NM_207294
Length: 171
Transcript: NM_207294
Exon
Length: 258
Transcript: NM_207294
Length: 258
Transcript: NM_207294
Exon
Length: 154
Transcript: NM_207294
Length: 154
Transcript: NM_207294
Exon
Length: 95
Transcript: NM_207294
Length: 95
Transcript: NM_207294
Exon
Length: 75
Transcript: NM_207294
Length: 75
Transcript: NM_207294
Exon
Length: 3396
Transcript: NM_207294
Length: 3396
Transcript: NM_207294
Intron
Length: 114573
Transcript: NM_207294
Length: 114573
Transcript: NM_207294
Intron
Length: 30170
Transcript: NM_207294
Length: 30170
Transcript: NM_207294
Intron
Length: 2080
Transcript: NM_207294
Length: 2080
Transcript: NM_207294
Intron
Length: 8493
Transcript: NM_207294
Length: 8493
Transcript: NM_207294
Intron
Length: 2909
Transcript: NM_207294
Length: 2909
Transcript: NM_207294
Intron
Length: 3039
Transcript: NM_207294
Length: 3039
Transcript: NM_207294
Reaction
Expected peak: 298
83.3M
refseq_MBNL1.F3
refseq_MBNL1.R3
Expected peak: 298
83.3M
refseq_MBNL1.F3
refseq_MBNL1.R3
Exon
Length: 963
Transcript: NM_207295
Length: 963
Transcript: NM_207295
Exon
Length: 171
Transcript: NM_207295
Length: 171
Transcript: NM_207295
Exon
Length: 258
Transcript: NM_207295
Length: 258
Transcript: NM_207295
Exon
Length: 154
Transcript: NM_207295
Length: 154
Transcript: NM_207295
Exon
Length: 36
Transcript: NM_207295
Length: 36
Transcript: NM_207295
Exon
Length: 95
Transcript: NM_207295
Length: 95
Transcript: NM_207295
Exon
Length: 75
Transcript: NM_207295
Length: 75
Transcript: NM_207295
Exon
Length: 3396
Transcript: NM_207295
Length: 3396
Transcript: NM_207295
Intron
Length: 114573
Transcript: NM_207295
Length: 114573
Transcript: NM_207295
Intron
Length: 30170
Transcript: NM_207295
Length: 30170
Transcript: NM_207295
Intron
Length: 2080
Transcript: NM_207295
Length: 2080
Transcript: NM_207295
Intron
Length: 7768
Transcript: NM_207295
Length: 7768
Transcript: NM_207295
Intron
Length: 689
Transcript: NM_207295
Length: 689
Transcript: NM_207295
Intron
Length: 2909
Transcript: NM_207295
Length: 2909
Transcript: NM_207295
Intron
Length: 3039
Transcript: NM_207295
Length: 3039
Transcript: NM_207295
Reaction
Expected peak: 298
83.3M
refseq_MBNL1.F3
refseq_MBNL1.R3
Expected peak: 298
83.3M
refseq_MBNL1.F3
refseq_MBNL1.R3
Exon
Length: 963
Transcript: NM_207296
Length: 963
Transcript: NM_207296
Exon
Length: 171
Transcript: NM_207296
Length: 171
Transcript: NM_207296
Exon
Length: 204
Transcript: NM_207296
Length: 204
Transcript: NM_207296
Exon
Length: 258
Transcript: NM_207296
Length: 258
Transcript: NM_207296
Exon
Length: 95
Transcript: NM_207296
Length: 95
Transcript: NM_207296
Exon
Length: 64
Transcript: NM_207296
Length: 64
Transcript: NM_207296
Exon
Length: 75
Transcript: NM_207296
Length: 75
Transcript: NM_207296
Exon
Length: 3396
Transcript: NM_207296
Length: 3396
Transcript: NM_207296
Intron
Length: 114573
Transcript: NM_207296
Length: 114573
Transcript: NM_207296
Intron
Length: 17605
Transcript: NM_207296
Length: 17605
Transcript: NM_207296
Intron
Length: 12361
Transcript: NM_207296
Length: 12361
Transcript: NM_207296
Intron
Length: 10727
Transcript: NM_207296
Length: 10727
Transcript: NM_207296
Intron
Length: 1768
Transcript: NM_207296
Length: 1768
Transcript: NM_207296
Intron
Length: 1077
Transcript: NM_207296
Length: 1077
Transcript: NM_207296
Intron
Length: 3039
Transcript: NM_207296
Length: 3039
Transcript: NM_207296
Exon
Length: 963
Transcript: NM_207297
Length: 963
Transcript: NM_207297
Exon
Length: 171
Transcript: NM_207297
Length: 171
Transcript: NM_207297
Exon
Length: 204
Transcript: NM_207297
Length: 204
Transcript: NM_207297
Exon
Length: 258
Transcript: NM_207297
Length: 258
Transcript: NM_207297
Exon
Length: 154
Transcript: NM_207297
Length: 154
Transcript: NM_207297
Exon
Length: 36
Transcript: NM_207297
Length: 36
Transcript: NM_207297
Exon
Length: 75
Transcript: NM_207297
Length: 75
Transcript: NM_207297
Exon
Length: 3396
Transcript: NM_207297
Length: 3396
Transcript: NM_207297
Intron
Length: 114573
Transcript: NM_207297
Length: 114573
Transcript: NM_207297
Intron
Length: 17605
Transcript: NM_207297
Length: 17605
Transcript: NM_207297
Intron
Length: 12361
Transcript: NM_207297
Length: 12361
Transcript: NM_207297
Intron
Length: 2080
Transcript: NM_207297
Length: 2080
Transcript: NM_207297
Intron
Length: 7768
Transcript: NM_207297
Length: 7768
Transcript: NM_207297
Intron
Length: 3693
Transcript: NM_207297
Length: 3693
Transcript: NM_207297
Intron
Length: 3039
Transcript: NM_207297
Length: 3039
Transcript: NM_207297
Reaction
Expected peak: 298
83.3M
refseq_MBNL1.F3
refseq_MBNL1.R3
Expected peak: 298
83.3M
refseq_MBNL1.F3
refseq_MBNL1.R3
refseq_MBNL1.F3
GATTGTCGGTTTGCTCATCC
GATTGTCGGTTTGCTCATCC
refseq_MBNL1.R3
CAGTGTTAAAGACTGCGGTGG
CAGTGTTAAAGACTGCGGTGG