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Sequences

Sequence NM_198244

GAAGCGGTGGCCGCCGAGCGGGATCTGTGCGGGGAGCCGGAAATGGTTGTGGACTACGTCTGTGCGGCTGCGTGGGGCTCGGCCGCGCGGACTGAAGGAG ACTGAAGCACTTCTACCCTAGCCGGGCCCAGCCCCCGAGCAGTGCAGCCTCCCGAGTGCAGAGTGCAGCCCCTGCCCGCCCTGGCCCAGCTGCCCATGTC TACCCTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGATCTCCTACCCAGCCTCCCAGGGGGCCTACTACATCCCTGGACAGGGGCGTTCCACATACG TTGTCCCGACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCAAGCCCTACAGAATTTGGGACCTACGCTGGCGCCTACTATCC AGCCCAAGGGGTGCAGCAGTTTCCCACTGGCGTGGCCCCCACCCCAGTTTTGATGAACCAGCCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATC CGAATTCGAGATCCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCG GTCTGGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGCCAGATGACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCT GCCTGGCCCAGAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGACCCCATCACCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTC GCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAAGAATCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCT CTCCAGAACCCACTCCTCTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTCCTCTCCAGGC TCCCACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGTCCCATCTGAAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTT GCTCCTCCCCCAGCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCAACTGCCCAACCTGAGGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGG ACTTAAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAATGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTACGGCTCCTTC AGCTACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGAAGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGA GAGGAACTGCTCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCTCAGAATTTGGAGGCAGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGA GGAGACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGACCTTCTGGATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTGGAAAATCA GCCTCCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCCACGTCCTGAGGAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAA ATTCACAATGCTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATATAAGTCAGATCAGTGGAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACC GTGAGTTCCTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCAGAGGGATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCCAATAAAAC ACCACTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGGCCCAGACTTCACTCCATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACC CGTGGGCCCCCAAGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGCCTGGGACCCCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGCA AGATCATTGCCACAGTGTTAATGACCGAAGATATAAAACTGAACAAAGCAGAGAAAGCCTGGAAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCG AGGGGAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTGCGCTCCATCCTGAATAAACTGACACCCCAGATGTTCCAGCAGCTGATG AAGCAAGTGACGCAGCTGGCCATCGACACCGAGGAACGCCTCAAAGGGGTCATTGACCTCATTTTTGAGAAGGCCATTTCAGAGCCCAACTTCTCTGTGG CCTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACGGAAAAGCCAACAGTGACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATG TCAGAAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAGCAAAAAGAGATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTG AAGGAAGAGCTGGAAGAGGCTCGGGACATAGCCCGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTGTTCAAACTGAAGATGTTAACAGAGG CAATAATGCATGACTGTGTGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCCTTGAGTGCCTTTGTCGTCTGCTCACCACCATTGGCAAAGACCTGGA CTTTGAAAAAGCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAAATCATTAAAGAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAG GACGTGCTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGGGATCAGGGTCCCAAGACCATTGACCAGATCCATAAGGAGGCTGAGATGGAAGAAC ATCGAGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGCAGTGACAAGCGTCGGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGT GGATGATGGTGGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGACACCTCACGACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCT AACAACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGGCAGCAGCGGAGGCTCAGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTC GCCCAGCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAACAAGCGGTACCCACAGAAAGCACAGATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAG CCGAGAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGAACGGGGAGGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCT GCTACCAAGCGGAGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGAGAACGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAGGATC GGGACCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCCCCCTGAAGGCGGCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAA GGCTATCATTGAGGAATATCTCCATCTCAATGACATGAAAGAGGCAGTCCAGTGCGTGCAGGAGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGG CATGGTGTCGAGTCTACGCTGGAGCGCAGTGCCATTGCTCGTGAGCATATGGGGCAGCTGCTGCACCAGCTGCTCTGTGCTGGGCATCTGTCTACTGCTC AGTACTACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAGGACATGGAAATTGACATCCCCCACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCAT TCTGCAGGAAGGTGGGGTGCCCATGGGGGAGCTGTTCAGGGAGATTACAAAGCCTCTGAGACCGTTGGGCAAAGCTGCTTCCCTGTTGCTGGAGATCCTG GGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAAGCCGGGCTTAGCTGGAAGGAATTTCTACCTGAAGGCCAGGACATTG GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCGGAAGCCCCTGGCCAGAGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCT GGAGAAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATAGAGGCCAACCTGAGTGAGCAGCAGATAGTATCCAACACGTTAGTTCGA GCCCTCATGACGGCTGTCTGCTATTCTGCAATTATTTTTGAGACTCCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGAGCGAAGCTGCTGCAGAAAT ACCTGTGTGACGAGCAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGA CGCACTGTATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTTGGGAGAGTAGCAAGGACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAA TCTGTCACAGCCTTCTTCAAGTGGCTCCGTGAAGCAGAGGAGGAGTCTGACCACAACTGAGGGCTGGTGGGGCCGGGGACCTGGAGCCCCATGGACACAC AGATGGCCCGGCTAGCCGCCTGGACTGCAGGGGGGCGGCAGCAGCGGCGGTGGCAGTGGGTGCCTGTAGTGTGATGTGTCTGAACTAATAAAGTGGCTGA AGAGGCAGGATGGCTTGGGGCTGCCTGGGCCCCCCTCCAGGATGCCGCCAGGTGTCCCTCTCCTCCCCCTGGGGCACAGAGATATATTATATATAAAGTC TTGAAATTTGGTGTGTCTTGGGGTGGGGAGGGGCACCAACGCCTGCCCCTGGGGTCCTTTTTTTTATTTTCTGAAAATCACTCTCGGGACTGCCGTCCTC GCTGCTGGGGGCATATGCCCCAGCCCCTGTACCACCCCTGCTGTTGCCTGGGCAGGGGGAAGGGGGGGCACGGTGCCTGTAATTATTAAACATGAATTCA ATTAAGCTCA

refseq_EIF4G1.F1 (forward primer)
refseq_EIF4G1.R1 (reverse primer)
Multiple exon skipping

Frame 0

EAVAAERDLCGEPEMVVDYVCAAAWGSAARTEG D*STSTLAGPSPRAVQPPECRVQPLPALAQLPMS TLLDPK***SLPRSPTQPPRGPTTSLDRGVPHT LSRHSSTLCSQEPQASIQVQALQNLGPTLAPTI QPKGCSSFPLAWPPPQF**TSHPRLLPRGSVRRS EFEIQTKEERISQRRSCLGPALPPHPPLPRREA VWSLKLMGRRPRLLSLSGQMTGHREQSLLTGQG CLAQSIALQNPSLRRLLRPHHHPQSWNRGLSLIS QSSLFLGTL*QLYKCL*KNQPPSPVKLGSHIAS LQNPLLSPNPYWK*K*HLANRFQNLSFLPVLSR LPPLWHLTQWKFMSLMAWSHLKIWNQRWSQAQSL LLPQLAPPNPLCPLLQLPNLRNCSTEPPRHQLW T*AQSVSQRSRPRR*QHQWRPPPSPLLLQLRLL QLLPQLRRRKWKKKKKRKKEKQEKQEKLRVRKEE RNCSPQRVPLFQPTCLRIWRQQQPLKWQYLCQR GDGKLRS*IRRRLLETFWMPSRRRTRQYQRWKI SLLQAAIQAQSLRAVVCPHVLRKQMRPGTQRKTK FTMLRTSSPGNRSMNISQISGSL*T*RRKNVTT VSSCLVFSSSLPVCRSQRDCHISVTWCWTRPIK HHCGHWIPLDYKA*IVAQTSLHPLPTLAGQPLAP VGPQGVGQVGSCPVGRLAWDPGALSRDPEKNHA RSLPQC**PKI*N*TKQRKPGNPAASGRRLIRI EGKKMLMAAKPRTYSAGCAPS*IN*HPRCSSS** SK*RSWPSTPRNASKGSLTSFLRRPFQSPTSLW PMPTCAAASWR*KCPLRKSQQ*L*TSESCC*ID VRRSLRKTKMMMRFLRRSKKRWMKLLRQRNEDA* RKSWKRLGT*PGGAL*GISSLLESCSN*RC*QR Q*CMTVWSNCLRTMMKSPLSAFVVCSPPLAKTW TLKKPSPEWISISTRWKKSLKKRRRHPASALCCR TCWICEGAIGCHAEGIRVPRPLTRSIRRLRWKN IESTSKCSSSWPRAVTSVGAVLQALPSAVDFPL WMMVAGTQFPSAKVAAPLTPHDSPRSPSLAPSIL TTSSLHLEGD*AGARAAAEAQEPSPQTQHQKLL AQLLVL*IASQPFNKRYPQKAQIIDVWCRGVA* AENEARKLETEETA*SGVNGEGTVGTGLIVRGHL LPSGASARKWRSGVENGPPSLRGCARQLASRRI GTVGGMP*SEKLPYPQ*AP*RRLSLRRS*RRNP RLSLRNISISMT*KRQSSACRSWPHPPCSSSLYG MVSSLRWSAVPLLVSIWGSCCTSCSVLGICLLL STTKGCMKSWNWLRTWKLTSPTCGST*RNW*HP FCRKVGCPWGSCSGRLQSL*DRWAKLLPCCWRSW ASCAKAWVLKRWGRCGEKPGLAGRNFYLKARTL VHSSLNRRWSIPWERSRKPLARGHSPPRS*TGS WRSC*RRAAVTSGCSTG*RPT*VSSR*YPTR*FE PS*RLSAILQLFLRLPSEWTLQC*KRERSCCRN TCVTSRRSYRRSTPSRPL**P*NSLPTCCGCSL THCMTRTW*RRMPSTVGRVARTPLSSRARVWPLN LSQPSSSGSVKQRRSLTTTEGWWGRGPGAPWTH RWPG*PPGLQGGGSSGGGSGCL*CDVSELIKWL KRQDGLGLPGPPSRMPPGVPLLPLGHRDILYIKS *NLVCLGVGRGTNACPWGPFFYFLKITLGTAVL AAGGICPSPCTTPAVAWAGGRGGTVPVIIKHEF N*A

Frame 1

KRWPPSGICAGSRKWLWTTSVRLRGARPRGLKE TEALLP*PGPAPEQCSLPSAECSPCPPWPSCPC LPCWIPSNDDPFPDLLPSLPGGLLHPWTGAFHIR CPDTAVPCAARSPRLLSRCKPYRIWDLRWRLLS SPRGAAVSHWRGPHPSFDEPATPDCSQEGA*DD PNSRSKPRRKGYHRGDHVWGPHCLHTHPSPDGRR SGASS*WGDAPGCCHCPAR*PVTGSNHC*PARA AWPRA*PFRIPAFVAFSDPITIPSLGTGV*A*S RSPLYSWGHYDNYTNVCRRINPHLP*NWGAISPL SRTHSSRRTHTGSRSDT*QTGSRI*VFFQSSPG SHPFGISHSGNS*A*WHGPI*RSGTRGGVKPRA CSSPSLPLRIPCAHCSNCPT*GTAQRSPLATSCG LKPSQ*ARGAGQGGDSINGAPHHPLCYSSYGSF SYFPSSGGGNGRRRRRGRRRSRRSRRS*E*ERR RGTAPPREYPYSSQLVSEFGGSSSHSSGSICAKE ETEN*GAK*EGGCWRPSGCLQGGEPGSTRGGKS ASCRQQSRPRV*GQWCAPTS*GSR*DLGLKGRQ NSQC*EHPARGTEV*I*VRSVEASKPRGEKTLRP *VPAWFSVHLCQYAEARGIATYQ*RGAGQGQ*N TTAATGSH*TTRHKLWPRLHSILCQPWPDNP*H PWAPKGWARWGAAPWAGWPGTPALSAGTPKRTTQ DHCHSVNDRRYKTEQSRESLETQQQADGG**GS RGRRC*WQQNPGPIPQGALHPE*TDTPDVPAAD EASDAAGHRHRGTPQRGH*PHF*EGHFRAQLLCG LCQHVPLPHGAESAHYGKANSDCELPKAVVESM SEGV*ERQR***GF*EEAKRDG*SCYGRGTRTP EGRAGRGSGHSPAALFREYQVYWRVVQTEDVNRG NNA*LCGQTA*EP**RVP*VPLSSAHHHWQRPG L*KSQAPNGSVFQPDGKNH*RKEDVIPHPLYAA GRAGSAREQLGATPRGSGSQDH*PDP*GG*DGRT SRAHQSAAAHGQGQ*QASGRSSRPSHQPWTSPC G*WWLEHSSHQQR*PPH*HLTTHQDHQAWLHRF *QPALCTWRATELGQGQQRRLRSQALRRSIRSCS PSY*YFESLLSPSTSGTHRKHR**TCGAEE*LE PRTRRESWRPRRPPRAE*TGRGPWGPA*SCADT CYQAELQQGSGGAE*RTALPA*GAAQGS*PHGGS GPWAGCREARSCPTPSEPPEGGSL*GGVREEIQ GYH*GISPSQ*HERGSPVRAGAGLTLLALHLCT AWCRVYAGAQCHCS*AYGAAAAPAALCWASVYCS VLPRVV*NLGIG*GHGN*HPPRVALPSGTGNTH SAGRWGAHGGAVQGDYKASETVGQSCFPVAGDP GPPVQKHGS*KGGDAVARSRA*LEGIST*RPGHW CIRR*TEGGVYPGRGVGSPWPEGTPLRGAEQAA GEAAEGGQQ*PAGVRLDRGQPE*AADSIQHVSS SPHDGCLLFCNYF*DSPPSGRCSAESASEAAAEI PV*RAEGATGALRPPGPCSDLRTASQPAADVL* RTV*RGRGEGGCLLQLGE*QGPR*AAGQGCGP* ICHSLLQVAP*SRGGV*PQLRAGGAGDLEPHGHT DGPASRLDCRGAAAAAVAVGACSVMCLN**SG* RGRMAWGCLGPPPGCRQVSLSSPWGTEIYYI*S LEIWCVLGWGGAPTPAPGVLFFIF*KSLSGLPSS LLGAYAPAPVPPLLLPGQGEGGARCL*LLNMNS IKL

Frame 2

SGGRRAGSVRGAGNGCGLRLCGCVGLGRAD*RR LKHFYPSRAQPPSSAASRVQSAAPARPGPAAHV YPAGSQVMMIPSQISYPASQGAYYIPGQGRSTY VVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYP AQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTI RIRDPNQGGKDITEEIMSGARTASTPTPPQTGG GLEPQANGETPQVAVIVRPDDRSQGAIIADRPGL PGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNL AVLSIPGDTMTTIQMSVEESTPISRETGEPYRL SPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQA PTPLASHTVEIHEPNGMVPSEDLEPEVESSPEL APPPACPSESPVPIAPTAQPEELLNGAPSPPAV DLSPVSEPEEQAKEVTASMAPPTIPSATPATAPS ATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGG EELLPPESTPIPANLSQNLEAAAATQVAVSVPK RRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQ PPAGSNPGPESEGSGVPPRPEEADETWDSKEDK IHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYD REFLLGFQFIFASMQKPEGLPHISDVVLDKANKT PLRPLDPTRLQGINCGPDFTPSFANLGRTTLST RGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPR KIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDR GEEDADGSKTQDLFRRVRSILNKLTPQMFQQLM KQVTQLAIDTEERLKGVIDLIFEKAISEPNFSV AYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRC QKEFEKDKDDDEVFEKKQKEMDEAATAEERGRL KEELEEARDIARRRSLGNIKFIGELFKLKMLTE AIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLD FEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQ DVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEE HREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLV DDGGWNTVPISKGSRPIDTSRLTKITKPGSIDS NNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAA RPATSTLNRFSALQQAVPTESTDNRRVVQRSSLS RERGEKAGDRGDRLERSERGGDRGDRLDRARTP ATKRSFSKEVEERSRERPSQPEGLRKAASLTED RDRGRDAVKREAALPPVSPLKAALSEEELEKKSK AIIEEYLHLNDMKEAVQCVQELASPSLLFIFVR HGVESTLERSAIAREHMGQLLHQLLCAGHLSTA QYYQGLYEILELAEDMEIDIPHVWLYLAELVTPI LQEGGVPMGELFREITKPLRPLGKAASLLLEIL GLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDI GAFVAEQKVEYTLGEESEAPGQRALPSEELNRQL EKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVR ALMTAVCYSAIIFETPLRVDVAVLKARAKLLQK YLCDEQKELQALYALQALVVTLEQPPNLLRMFFD ALYDEDVVKEDAFYSWESSKDPAEQQGKGVALK SVTAFFKWLREAEEESDHN*GLVGPGTWSPMDT QMARLAAWTAGGRQQRRWQWVPVV*CV*TNKVAE EAGWLGAAWAPLQDAARCPSPPPGAQRYIIYKV LKFGVSWGGEGHQRLPLGSFFLFSENHSRDCRP RCWGHMPQPLYHPCCCLGRGKGGHGACNY*T*IQ LSS

Sequence NM_198241

GAAGCGGTGGCCGCCGAGCGGGATCTGTGCGGGGAGCCGGAAATGGTTGTGGACTACGTCTGTGCGGCTGCGTGGGGCTCGGCCGCGCGGACTGAAGGAG ACTGAAGGCCCTCGGATGCCCAGAACCTGTAGGCCGCACCGTGGACTTGTTCTTAATCGAGGGGGTGCTGGGGGGACCCTGATGTGGCACCAAATGAAAT GAACAAAGCTCCACAGTCCACAGGCCCCCCACCCGCCCCATCCCCCGGACTCCCACAGCCAGCGTTTCCCCCGGGGCAGACAGCGCCGGTGGTGTTCAGT ACGCCACAAGCGACACAAATGAACACGCCTTCTCAGCCCCGCCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCGAGCAGTGCAGCCTCCCGAGTGCAGA GTGCAGCCCCTGCCCGCCCTGGCCCAGCTGCCCATGTCTACCCTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGATCTCCTACCCAGCCTCCCAGGG GGCCTACTACATCCCTGGACAGGGGCGTTCCACATACGTTGTCCCGACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCAAGC CCTACAGAATTTGGGACCTACGCTGGCGCCTACTATCCAGCCCAAGGGGTGCAGCAGTTTCCCACTGGCGTGGCCCCCACCCCAGTTTTGATGAACCAGC CACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATCCGAATTCGAGATCCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCTGGGGCCCG CACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGCCAGATGAC CGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGACCCCATCAC CATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTCGCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAAGAATCAAC CCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCTCTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTAGCAAACCG GTTCCAGAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGTCCCATCTG AAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCAACTGCCCAACCTGA GGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGGACTTAAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAATGGCGCCC CCCACCATCCCCTCTGCTACTCCAGCTACGGCTCCTTCAGCTACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGAAGGAGAAG CAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCTCAGAATTTGGAGGC AGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGAGGAGACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGACCTTCTGGATGCCTTC AAGGAGGCGAACCCGGCAGTACCAGAGGTGGAAAATCAGCCTCCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCCACGTCCTG AGGAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAAATTCACAATGCTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATATAAGTCAGATCAGTG GAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACCGTGAGTTCCTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCAGAGGGATTGCCA CATATCAGTGACGTGGTGCTGGACAAGGCCAATAAAACACCACTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGGCCCAGACTTCACTC CATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACCCGTGGGCCCCCAAGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGCCTGGGACC CCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGCAAGATCATTGCCACAGTGTTAATGACCGAAGATATAAAACTGAACAAAGCAGAGAAAGCCTGG AAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCGAGGGGAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTGCGCTCCATCC TGAATAAACTGACACCCCAGATGTTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGCCATCGACACCGAGGAACGCCTCAAAGGGGTCATTGACCTCAT TTTTGAGAAGGCCATTTCAGAGCCCAACTTCTCTGTGGCCTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACGGAAAAGCCAACA GTGACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGTCAGAAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAGCAAAAAGAGA TGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGCTCGGGACATAGCCCGGCGGCGCTCTTTAGGGAATATCAAGTT TATTGGAGAGTTGTTCAAACTGAAGATGTTAACAGAGGCAATAATGCATGACTGTGTGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCCTTGAGTGC CTTTGTCGTCTGCTCACCACCATTGGCAAAGACCTGGACTTTGAAAAAGCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAAATCATTAAAG AAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAGGACGTGCTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGGGATCAGGGTCCCAAGAC CATTGACCAGATCCATAAGGAGGCTGAGATGGAAGAACATCGAGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGCAGTGACAAGCGTCGGGGCGGT CCTCCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGATGATGGTGGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGACACCTCAC GACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCTAACAACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGGCAGCAGCGGAGGCTC AGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTCGCCCAGCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAACAAGCGGTACCCACAGAAAGC ACAGATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAGCCGAGAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGAACGGGGAG GGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGAGAACGGCCCTCCCAGCC TGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAGGATCGGGACCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCCCCCTGAAG GCGGCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAAGGCTATCATTGAGGAATATCTCCATCTCAATGACATGAAAGAGGCAGTCCAGTGCGTGCAGG AGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGGCATGGTGTCGAGTCTACGCTGGAGCGCAGTGCCATTGCTCGTGAGCATATGGGGCAGCTGCT GCACCAGCTGCTCTGTGCTGGGCATCTGTCTACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAGGACATGGAAATTGACATCCCC CACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCATTCTGCAGGAAGGTGGGGTGCCCATGGGGGAGCTGTTCAGGGAGATTACAAAGCCTCTGAGAC CGTTGGGCAAAGCTGCTTCCCTGTTGCTGGAGATCCTGGGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAAGCCGGGCT TAGCTGGAAGGAATTTCTACCTGAAGGCCAGGACATTGGTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCGGAAGCCCCTGGC CAGAGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCTGGAGAAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATAGAGGCCAACC TGAGTGAGCAGCAGATAGTATCCAACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGCTATTCTGCAATTATTTTTGAGACTCCCCTCCGAGTGGACGT TGCAGTGCTGAAAGCGCGAGCGAAGCTGCTGCAGAAATACCTGTGTGACGAGCAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTGTAGTGACC TTAGAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGACGCACTGTATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTTGGGAGAGTAGCAAGG ACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCCTTCTTCAAGTGGCTCCGTGAAGCAGAGGAGGAGTCTGACCACAACTGAGG GCTGGTGGGGCCGGGGACCTGGAGCCCCATGGACACACAGATGGCCCGGCTAGCCGCCTGGACTGCAGGGGGGCGGCAGCAGCGGCGGTGGCAGTGGGTG CCTGTAGTGTGATGTGTCTGAACTAATAAAGTGGCTGAAGAGGCAGGATGGCTTGGGGCTGCCTGGGCCCCCCTCCAGGATGCCGCCAGGTGTCCCTCTC CTCCCCCTGGGGCACAGAGATATATTATATATAAAGTCTTGAAATTTGGTGTGTCTTGGGGTGGGGAGGGGCACCAACGCCTGCCCCTGGGGTCCTTTTT TTTATTTTCTGAAAATCACTCTCGGGACTGCCGTCCTCGCTGCTGGGGGCATATGCCCCAGCCCCTGTACCACCCCTGCTGTTGCCTGGGCAGGGGGAAG GGGGGGCACGGTGCCTGTAATTATTAAACATGAATTCAATTAAGCTCA

refseq_EIF4G1.F1 (forward primer)
refseq_EIF4G1.R1 (reverse primer)
Multiple exon skipping

Frame 0

EAVAAERDLCGEPEMVVDYVCAAAWGSAARTEG D*RPSDAQNL*AAPWTCS*SRGCWGDPDVAPNEM NKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFS TPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQ SAAPARPGPAAHVYPAGSQVMMIPSQISYPASQG AYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGAS PTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQ PPQIAPKRERKTIRIRDPNQGGKDITEEIMSGAR TASTPTPPQTGGGLEPQANGETPQVAVIVRPDD RSQGAIIADRPGLPGPEHSPSESQPSSPSPTPS PSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEEST PISRETGEPYRLSPEPTPLAEPILEVEVTLSKP VPESEFSSSPLQAPTPLASHTVEIHEPNGMVPS EDLEPEVESSPELAPPPACPSESPVPIAPTAQPE ELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAP PTIPSATPATAPSATSPAQEEEMEEEEEEEEGE AGEAGEAESEKGGEELLPPESTPIPANLSQNLEA AAATQVAVSVPKRRRKIKELNKKEAVGDLLDAF KEANPAVPEVENQPPAGSNPGPESEGSGVPPRP EEADETWDSKEDKIHNAENIQPGEQKYEYKSDQW KPLNLEEKKRYDREFLLGFQFIFASMQKPEGLP HISDVVLDKANKTPLRPLDPTRLQGINCGPDFT PSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGP RRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAW KPSSKRTAADKDRGEEDADGSKTQDLFRRVRSI LNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLI FEKAISEPNFSVAYANMCRCLMALKVPTTEKPT VTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKE MDEAATAEERGRLKEELEEARDIARRRSLGNIKF IGELFKLKMLTEAIMHDCVVKLLKNHDEESLEC LCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIK EKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKT IDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGG PPGPPISRGLPLVDDGGWNTVPISKGSRPIDTS RLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGS GAKPSDAASEAARPATSTLNRFSALQQAVPTES TDNRRVVQRSSLSRERGEKAGDRGDRLERSERG GDRGDRLDRARTPATKRSFSKEVEERSRERPSQP EGLRKAASLTEDRDRGRDAVKREAALPPVSPLK AALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQ ELASPSLLFIFVRHGVESTLERSAIAREHMGQLL HQLLCAGHLSTAQYYQGLYEILELAEDMEIDIP HVWLYLAELVTPILQEGGVPMGELFREITKPLR PLGKAASLLLEILGLLCKSMGPKKVGTLWREAGL SWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPG QRALPSEELNRQLEKLLKEGSSNQRVFDWIEAN LSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDV AVLKARAKLLQKYLCDEQKELQALYALQALVVT LEQPPNLLRMFFDALYDEDVVKEDAFYSWESSK DPAEQQGKGVALKSVTAFFKWLREAEEESDHN*G LVGPGTWSPMDTQMARLAAWTAGGRQQRRWQWV PVV*CV*TNKVAEEAGWLGAAWAPLQDAARCPS PPPGAQRYIIYKVLKFGVSWGGEGHQRLPLGSFF LFSENHSRDCRPRCWGHMPQPLYHPCCCLGRGK GGHGACNY*T*IQLSS

Frame 1

KRWPPSGICAGSRKWLWTTSVRLRGARPRGLKE TEGPRMPRTCRPHRGLVLNRGGAGGTLMWHQMK *TKLHSPQAPHPPHPPDSHSQRFPRGRQRRWCSV RHKRHK*TRLLSPASTSTLAGPSPRAVQPPECR VQPLPALAQLPMSTLLDPK***SLPRSPTQPPR GPTTSLDRGVPHTLSRHSSTLCSQEPQASIQVQA LQNLGPTLAPTIQPKGCSSFPLAWPPPQF**TS HPRLLPRGSVRRSEFEIQTKEERISQRRSCLGP ALPPHPPLPRREAVWSLKLMGRRPRLLSLSGQMT GHREQSLLTGQGCLAQSIALQNPSLRRLLRPHH HPQSWNRGLSLISQSSLFLGTL*QLYKCL*KNQ PPSPVKLGSHIASLQNPLLSPNPYWK*K*HLANR FQNLSFLPVLSRLPPLWHLTQWKFMSLMAWSHL KIWNQRWSQAQSLLLPQLAPPNPLCPLLQLPNL RNCSTEPPRHQLWT*AQSVSQRSRPRR*QHQWRP PPSPLLLQLRLLQLLPQLRRRKWKKKKKRKKEK QEKQEKLRVRKEERNCSPQRVPLFQPTCLRIWR QQQPLKWQYLCQRGDGKLRS*IRRRLLETFWMPS RRRTRQYQRWKISLLQAAIQAQSLRAVVCPHVL RKQMRPGTQRKTKFTMLRTSSPGNRSMNISQIS GSL*T*RRKNVTTVSSCLVFSSSLPVCRSQRDCH ISVTWCWTRPIKHHCGHWIPLDYKA*IVAQTSL HPLPTLAGQPLAPVGPQGVGQVGSCPVGRLAWD PGALSRDPEKNHARSLPQC**PKI*N*TKQRKPG NPAASGRRLIRIEGKKMLMAAKPRTYSAGCAPS *IN*HPRCSSS**SK*RSWPSTPRNASKGSLTS FLRRPFQSPTSLWPMPTCAAASWR*KCPLRKSQQ *L*TSESCC*IDVRRSLRKTKMMMRFLRRSKKR WMKLLRQRNEDA*RKSWKRLGT*PGGAL*GISS LLESCSN*RC*QRQ*CMTVWSNCLRTMMKSPLSA FVVCSPPLAKTWTLKKPSPEWISISTRWKKSLK KRRRHPASALCCRTCWICEGAIGCHAEGIRVPR PLTRSIRRLRWKNIESTSKCSSSWPRAVTSVGAV LQALPSAVDFPLWMMVAGTQFPSAKVAAPLTPH DSPRSPSLAPSILTTSSLHLEGD*AGARAAAEA QEPSPQTQHQKLLAQLLVL*IASQPFNKRYPQKA QIIDVWCRGVA*AENEARKLETEETA*SGVNGE GTVGTGLIVRGHLLPSGASARKWRSGVENGPPS LRGCARQLASRRIGTVGGMP*SEKLPYPQ*AP*R RLSLRRS*RRNPRLSLRNISISMT*KRQSSACR SWPHPPCSSSLYGMVSSLRWSAVPLLVSIWGSC CTSCSVLGICLLLSTTKGCMKSWNWLRTWKLTSP TCGST*RNW*HPFCRKVGCPWGSCSGRLQSL*D RWAKLLPCCWRSWASCAKAWVLKRWGRCGEKPG LAGRNFYLKARTLVHSSLNRRWSIPWERSRKPLA RGHSPPRS*TGSWRSC*RRAAVTSGCSTG*RPT *VSSR*YPTR*FEPS*RLSAILQLFLRLPSEWT LQC*KRERSCCRNTCVTSRRSYRRSTPSRPL**P *NSLPTCCGCSLTHCMTRTW*RRMPSTVGRVAR TPLSSRARVWPLNLSQPSSSGSVKQRRSLTTTE GWWGRGPGAPWTHRWPG*PPGLQGGGSSGGGSGC L*CDVSELIKWLKRQDGLGLPGPPSRMPPGVPL LPLGHRDILYIKS*NLVCLGVGRGTNACPWGPF FYFLKITLGTAVLAAGGICPSPCTTPAVAWAGGR GGTVPVIIKHEFN*A

Frame 2

SGGRRAGSVRGAGNGCGLRLCGCVGLGRAD*RR LKALGCPEPVGRTVDLFLIEGVLGGP*CGTK*N EQSSTVHRPPTRPIPRTPTASVSPGADSAGGVQ YATSDTNEHAFSAPPALLP*PGPAPEQCSLPSAE CSPCPPWPSCPCLPCWIPSNDDPFPDLLPSLPG GLLHPWTGAFHIRCPDTAVPCAARSPRLLSRCK PYRIWDLRWRLLSSPRGAAVSHWRGPHPSFDEPA TPDCSQEGA*DDPNSRSKPRRKGYHRGDHVWGP HCLHTHPSPDGRRSGASS*WGDAPGCCHCPAR* PVTGSNHC*PARAAWPRA*PFRIPAFVAFSDPIT IPSLGTGV*A*SRSPLYSWGHYDNYTNVCRRIN PHLP*NWGAISPLSRTHSSRRTHTGSRSDT*QT GSRI*VFFQSSPGSHPFGISHSGNS*A*WHGPI* RSGTRGGVKPRACSSPSLPLRIPCAHCSNCPT* GTAQRSPLATSCGLKPSQ*ARGAGQGGDSINGA PHHPLCYSSYGSFSYFPSSGGGNGRRRRRGRRRS RRSRRS*E*ERRRGTAPPREYPYSSQLVSEFGG SSSHSSGSICAKEETEN*GAK*EGGCWRPSGCL QGGEPGSTRGGKSASCRQQSRPRV*GQWCAPTS* GSR*DLGLKGRQNSQC*EHPARGTEV*I*VRSV EASKPRGEKTLRP*VPAWFSVHLCQYAEARGIA TYQ*RGAGQGQ*NTTAATGSH*TTRHKLWPRLHS ILCQPWPDNP*HPWAPKGWARWGAAPWAGWPGT PALSAGTPKRTTQDHCHSVNDRRYKTEQSRESL ETQQQADGG**GSRGRRC*WQQNPGPIPQGALHP E*TDTPDVPAADEASDAAGHRHRGTPQRGH*PH F*EGHFRAQLLCGLCQHVPLPHGAESAHYGKAN SDCELPKAVVESMSEGV*ERQR***GF*EEAKRD G*SCYGRGTRTPEGRAGRGSGHSPAALFREYQV YWRVVQTEDVNRGNNA*LCGQTA*EP**RVP*V PLSSAHHHWQRPGL*KSQAPNGSVFQPDGKNH*R KEDVIPHPLYAAGRAGSAREQLGATPRGSGSQD H*PDP*GG*DGRTSRAHQSAAAHGQGQ*QASGR SSRPSHQPWTSPCG*WWLEHSSHQQR*PPH*HLT THQDHQAWLHRF*QPALCTWRATELGQGQQRRL RSQALRRSIRSCSPSY*YFESLLSPSTSGTHRK HR**TCGAEE*LEPRTRRESWRPRRPPRAE*TGR GPWGPA*SCADTCYQAELQQGSGGAE*RTALPA *GAAQGS*PHGGSGPWAGCREARSCPTPSEPPE GGSL*GGVREEIQGYH*GISPSQ*HERGSPVRAG AGLTLLALHLCTAWCRVYAGAQCHCS*AYGAAA APAALCWASVYCSVLPRVV*NLGIG*GHGN*HP PRVALPSGTGNTHSAGRWGAHGGAVQGDYKASET VGQSCFPVAGDPGPPVQKHGS*KGGDAVARSRA *LEGIST*RPGHWCIRR*TEGGVYPGRGVGSPW PEGTPLRGAEQAAGEAAEGGQQ*PAGVRLDRGQP E*AADSIQHVSSSPHDGCLLFCNYF*DSPPSGR CSAESASEAAAEIPV*RAEGATGALRPPGPCSD LRTASQPAADVL*RTV*RGRGEGGCLLQLGE*QG PR*AAGQGCGP*ICHSLLQVAP*SRGGV*PQLR AGGAGDLEPHGHTDGPASRLDCRGAAAAAVAVG ACSVMCLN**SG*RGRMAWGCLGPPPGCRQVSLS SPWGTEIYYI*SLEIWCVLGWGGAPTPAPGVLF FIF*KSLSGLPSSLLGAYAPAPVPPLLLPGQGE GGARCL*LLNMNSIKL