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Sequences

Sequence NM_007324

ATGCTGAAGCGGAGGCGGCCGCTGCTCGAGGATCAAACAGAGCATACTGAATCAGCAGGACTGGCTGGTGGTGCAGCAGACATCATGAGTAAGCACCGAG AAGTCTGTTCCTTATCACGTGTGTAAGGGGAAAAAGGTTTAAACAAGTCTCTTAAGTGGTGTTTCCTCACCGATGGAGAATTACTTCCAAGCAGAAGCTT ACAACCTGGACAAGGTGTTAGATGAATTTGAACAAAACGAAGATGAAACAGTTTCTTCTACTTTATTGGATACAAAGTGGAATAAGATTCTAGATCCCCC TTCTCACCGGCTGTCATTTAACCCTACTTTGGCCAGTGTGAATGAATCTGCAGTTTCTAATGAGTCACAACCACAACTGAAAGTCTTCTCCCTGGCTCAT TCAGCTCCCCTGACCACAGAGGAAGAGGATCACTGTGCTAATGGACAGGACTGTAATCTAAATCCAGAGATTGCCACAATGTGGATTGATGAAAATGCTG TTGCAGAAGACCAGTTAATTAAGAGAAACTATAGTTGGGATGATCAATGCAGTGCTGTTGAAGTGGGAGAGAAGAAATGTGGAAACCTGGCTTGTCTGCC AGATGAGAAGAATGTTCTTGTTGTAGCCGTCATGCATAACTGTGATAAAAGGACATTACAAAACGATTTACAGGATTGTAATAATTATAATAGTCAATCC CTTATGGATGCTTTTAGCTGTTCACTGGATAATGAAAACAGACAAACTGATCAATTTAGTTTTAGTATAAATGAGTCCACTGAAAAAGATATGAATTCAG AGAAACAAATGGATCCATTGAATAGACCGAAAACAGAGGGGAGATCTGTTAACCATCTGTGTCCTACTTCATCTGATAGTCTAGCCAGTGTCTGTTCCCC TTCACAATTAAAGGATGACGGAAGTATAGGTAGAGACCCCTCCATGTCTGCGATTACAAGTTTAACGGTTGATTCAGTAATCTCATCCCAGGGAACAGAT GGATGTCCTGCTGTTAAAAAGCAAGAGAACTATATACCAGATGAGGACCTCACTGGCAAAATCAGCTCTCCTAGGACAGATCTAGGGAGTCCAAATTCCT TTTCCCACATGAGTGAGGGGATTTTGATGAAAAAAGAGCCAGCAGAGGAGAGCACCACTGAAGAATCCCTCCGGTCTGGTTTACCTTTGCTTCTCAAACC AGACATGCCTAATGGGTCTGGAAGGAATAATGACTGTGAACGGTGTTCAGATTGCCTTGTGCCTAATGAAGTTAGGGCTGATGAAAATGAAGGTTATGAA CATGAAGAAACTCTTGGCACTACAGAATTCCTTAATATGACAGAGCATTTCTCTGAATCTCAGGACATGACTAATTGGAAGTTGACTAAACTAAATGAGA TGAATGATAGCCAAGTAAACGAAGAAAAGGAAAAGTTTCTACAGATTAGTCAGCCTGAGGACACTAATGGTGATAGTGGAGGACAGTGTGTTGGATTGGC AGATGCAGGTCTAGATTTAAAAGGAACTTGCATTAGTGAAAGTGAAGAATGTGATTTCTCCACTGTTATAGACACACCAGCAGCAAATTATCTATCTAAT GGTTGTGATTCCTATGGAATGCAAGACCCAGGTGTTTCTTTTGTTCCAAAGACTTTACCCTCCAAAGAAGATTCAGTAACAGAAGAAAAAGAAATAGAGG AAAGCAAGTCAGAATGCTACTCAAATATTTATGAACAGAGAGGAAATGAGGCCACAGAAGGGAGTGGACTACTTTTAAACAGCACTGGTGACCTAATGAA GAAAAATTATTTACATAATTTCTGTAGTCAAGTTCCATCAGTGCTTGGGCAATCTTCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTT GGTGGTGCAAGACCCAAGCAACCTTCTAATCTTAAACTTCAAATTCCAAAGCCATTATCAGACCATTTACAAAATGACTTTCCTGCAAACAGTGGAAATA ATACTAAAAATAAAAATGATATTCTTGGGAAAGCAAAATTAGGGGAAAACTCAGCAACCAATGTATGCAGTCCATCTTTGGGAAACATCTCTAATGTCGA TACAAATGGGGAACATTTAGAAAGTTATGAGGCTGAGATCTCCACTAGACCATGCCTTGCATTAGCTCCAGATAGCCCAGATAATGATCTCAGAGCTGGT CAGTTTGGAATTTCTGCCAGAAAGCCATTCACCACTCTGGGTGAGGTGGCTCCAGTATGGGTACCGGATTCTCAGGCTCCAAATTGCATGAAATGTGAAG CCAGGTTTACATTCACCAAAAGGAGGCATCACTGCAGAGCATGTGGGAAGGTTTTCTGTGCTTCCTGCTGTAGCCTGAAATGTAAACTGTTATACATGGA CAGAAAGGAAGCTAGAGTGTGTGTAATCTGCCATTCAGTGCTAATGAATGTGGCTCAGCCCAGAGAGCAGAGGCGAGTTTGGTTTGCTGATGGGATCTTG CCCAATGGAGAAGTTGCTGATGCAGCCAAATTAACAATGAATGGAACTTCCTCTGCAGGAACCCTGGCTGTGTCACACGACCCAGTCAAGCCAGTAACTA CCAGTCCTCTACCAGCAGAGACGGATATTTGTCTATTCTCTGGGAGTATAACTCAGGTTGGAAGTCCTGTTGGAAGTGCAATGAATCTTATTCCTGAAGA TGGCCTTCCTCCCATTCTCATCTCCACTGGTGTAAAAGGAGACTATGCTGTGGAAGAGAAACCATCACAGATTTCAGTAATGCAGCAGTTGGAGGATGGT GGCCCTGACCCACTTGTATTTGTTTTAAATGCAAATTTGTTGTCAATGGTTAAAATTGTAAATTATGTGAACAGGAAGTGCTGGTGTTTCACAACCAAGG GAATGCATGCAGTGGGTCAGTCTGAGATAGTCATTCTTCTACAGTGTTTACCGGATGAAAAGTGTTTGCCAAAGGATATCTTTAATCACTTTGTGCAGCT TTATCGGGATGCTCTGGCAGGGAATGTGGTGAGCAACTTGGGACATTCCTTCTTCAGTCAAAGTTTCCTTGGCAGTAAAGAACATGGTGGATTCTTATAT GTGACATCTACCTACCAGTCACTGCAAGACCTAGTACTCCCAACCCCACCTTACTTGTTTGGGATTCTTATCCAGAAATGGGAAACTCCTTGGGCTAAAG TATTTCCTATCCGTCTGATGTTGAGACTTGGAGCTGAATATCGACTTTATCCATGCCCACTATTCAGTGTCAGATTTCGGAAGCCATTGTTTGGAGAGAC GGGGCATACCATCATGAATCTTCTTGCAGACTTCAGAAATTACCAGTATACCTTGCCAGTAGTTCAAGGTTTGGTGGTTGATATGGAAGTTCGGAAAACT AGCATCAAAATTCCCAGCAACAGATACAATGAGATGATGAAAGCCATGAACAAGTCCAATGAGCATGTCCTGGCAGGAGGTGCCTGCTTCAATGAAAAGG CAGACTCTCATCTTGTGTGTGTACAGAATGATGATGGAAACTATCAGACCCAGGCTATCAGTATTCACAATCAGCCCAGAAAAGTGACTGGTGCCAGTTT CTTTGTGTTCAGTGGCGCTCTGAAATCCTCTTCTGGATACCTTGCCAAGTCCAGTATTGTGGAAGATGGTGTTATGGTCCAGATTACTGCAGAGAACATG GATTCCTTGAGGCAGGCACTGCGAGAGATGAAGGACTTCACCATCACCTGTGGGAAGGCGGACGCGGAGGAACCCCAGGAGCACATCCACATCCAGTGGG TGGATGATGACAAGAACGTTAGCAAGGGTGTCGTAAGTCCTATAGATGGGAAGTCCATGGAGACTATAACAAATGTGAAGATATTCCATGGATCAGAATA TAAAGCAAATGGAAAAGTAATCAGATGGACAGAGGTGTTTTTCCTAGAAAACGATGACCAGCACAATTGCCTCAGTGATCCTGCAGATCACAGTAGATTG ACTGAGCATGTTGCCAAAGCTTTTTGCCTTGCTCTCTGTCCTCACCTGAAACTTCTGAAGGAAGATGGAATGACCAAACTGGGACTACGTGTGACACTTG ACTCAGATCAGGTTGGCTATCAAGCAGGGAGCAATGGCCAGCCCCTTCCCTCGCAGTACATGAATGATCTGGACAGCGCCTTGGTGCCGGTGATCCATGG AGGGGCCTGCCAGCTTAGTGAGGGCCCCGTTGTCATGGAACTCATCTTTTATATTCTGGAAAACATCGTATAAACAGAGAAGACTTCATTTTTTTCTGTT CAGACTTGTTGCAACAGCAGTCATACCCAAATCATTTGCACTTTAAAACTGGAAGATTAAGCTTTTGTTAACACTATTAATGGGGTGGGGAATAGGGTGG GAGTGGGGGTTTGGGAGACGGGTGGGAAAGGGTGGTTGGGGGGACCGATGTTCCATAATTCTAAGTCTTCTATGCATTGTCCACCAAGAAGATCTGGGCA GCTTCTGTTCCTGCACAACAGTTATGCTATCCTTGCAGCTAATCCCCTTCTGTTACTGTTTAGACAAGAATTCCGCTCCTCTCTCAAGATTTACTTATGG TCATGTGCTCAGAAATGCTCAAATGGGTACAACCATCACCAAGGGTGGGATGGGAGGGCAGAGGGGAAATAAAATATAAAGCATCAGTTCTTGCACTCTT TGTACAGAATTGGTATAAAAAGGATAATTCCACACTGA

refseq_ZFYVE9.F2 (forward primer)
refseq_ZFYVE9.R2 (reverse primer)
Single exon skipping

Frame 0

MLKRRRPLLEDQTEHTESAGLAGGAADIMSKHR EVCSLSRV*GEKGLNKSLKWCFLTDGELLPSRSL QPGQGVR*I*TKRR*NSFFYFIGYKVE*DSRSP FSPAVI*PYFGQCE*ICSF**VTTTTESLLPGS FSSPDHRGRGSLC*WTGL*SKSRDCHNVD**KCC CRRPVN*EKL*LG*SMQCC*SGREEMWKPGLSA R*EECSCCSRHA*L**KDITKRFTGL**L**SI PYGCF*LFTG**KQTN*SI*F*YK*VH*KRYEFR ETNGSIE*TENRGEIC*PSVSYFI**SSQCLFP FTIKG*RKYR*RPLHVCDYKFNG*FSNLIPGNR WMSCC*KARELYTR*GPHWQNQLS*DRSRESKFL FPHE*GDFDEKRASRGEHH*RIPPVWFTFASQT RHA*WVWKE**L*TVFRLPCA**S*G**K*RL* T*RNSWHYRIP*YDRAFL*ISGHD*LEVD*TK*D E**PSKRRKGKVSTD*SA*GH*W**WRTVCWIG RCRSRFKRNLH**K*RM*FLHCYRHTSSKLSI* WL*FLWNARPRCFFCSKDFTLQRRFSNRRKRNRG KQVRMLLKYL*TERK*GHRREWTTFKQHW*PNE EKLFT*FL*SSSISAWAIFPQGSSKPAIYQCSF WWCKTQATF*S*TSNSKAIIRPFTK*LSCKQWK* Y*K*K*YSWESKIRGKLSNQCMQSIFGKHL*CR YKWGTFRKL*G*DLH*TMPCISSR*PR**SQSW SVWNFCQKAIHHSG*GGSSMGTGFSGSKLHEM*S QVYIHQKEASLQSMWEGFLCFLL*PEM*TVIHG QKGS*SVCNLPFSANECGSAQRAEASLVC*WDL AQWRSC*CSQINNEWNFLCRNPGCVTRPSQASNY QSSTSRDGYLSILWEYNSGWKSCWKCNESYS*R WPSSHSHLHWCKRRLCCGRETITDFSNAAVGGW WP*PTCICFKCKFVVNG*NCKLCEQEVLVFHNQG NACSGSV*DSHSSTVFTG*KVFAKGYL*SLCAA LSGCSGRECGEQLGTFLLQSKFPWQ*RTWWILI CDIYLPVTARPSTPNPTLLVWDSYPEMGNSLG*S ISYPSDVETWS*ISTLSMPTIQCQISEAIVWRD GAYHHESSCRLQKLPVYLASSSRFGG*YGSSEN *HQNSQQQIQ*DDESHEQVQ*ACPGRRCLLQ*KG RLSSCVCTE**WKLSDPGYQYSQSAQKSDWCQF LCVQWRSEILFWIPCQVQYCGRWCYGPDYCREH GFLEAGTARDEGLHHHLWEGGRGGTPGAHPHPVG G**QER*QGCRKSYRWEVHGDYNKCEDIPWIRI *SKWKSNQMDRGVFPRKR*PAQLPQ*SCRSQ*I D*ACCQSFLPCSLSSPETSEGRWNDQTGTTCDT* LRSGWLSSREQWPAPSLAVHE*SGQRLGAGDPW RGLPA**GPRCHGTHLLYSGKHRINREDFIFFC SDLLQQQSYPNHLHFKTGRLSFC*HY*WGGE*GG SGGLGDGWERVVGGTDVP*F*VFYALSTKKIWA ASVPAQQLCYPCS*SPSVTV*TRIPLLSQDLLM VMCSEMLKWVQPSPRVGWEGRGEIKYKASVLALF VQNWYKKDNSTL

Frame 1

C*SGGGRCSRIKQSILNQQDWLVVQQTS*VSTE KSVPYHVCKGKKV*TSLLSGVSSPMENYFQAEA YNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPP SHRLSFNPTLASVNESAVSNESQPQLKVFSLAH SAPLTTEEEDHCANGQDCNLNPEIATMWIDENA VAEDQLIKRNYSWDDQCSAVEVGEKKCGNLACLP DEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQS LMDAFSCSLDNENRQTDQFSFSINESTEKDMNS EKQMDPLNRPKTEGRSVNHLCPTSSDSLASVCSP SQLKDDGSIGRDPSMSAITSLTVDSVISSQGTD GCPAVKKQENYIPDEDLTGKISSPRTDLGSPNS FSHMSEGILMKKEPAEESTTEESLRSGLPLLLKP DMPNGSGRNNDCERCSDCLVPNEVRADENEGYE HEETLGTTEFLNMTEHFSESQDMTNWKLTKLNE MNDSQVNEEKEKFLQISQPEDTNGDSGGQCVGLA DAGLDLKGTCISESEECDFSTVIDTPAANYLSN GCDSYGMQDPGVSFVPKTLPSKEDSVTEEKEIE ESKSECYSNIYEQRGNEATEGSGLLLNSTGDLMK KNYLHNFCSQVPSVLGQSSPKVVASLPSISVPF GGARPKQPSNLKLQIPKPLSDHLQNDFPANSGN NTKNKNDILGKAKLGENSATNVCSPSLGNISNVD TNGEHLESYEAEISTRPCLALAPDSPDNDLRAG QFGISARKPFTTLGEVAPVWVPDSQAPNCMKCE ARFTFTKRRHHCRACGKVFCASCCSLKCKLLYMD RKEARVCVICHSVLMNVAQPREQRRVWFADGIL PNGEVADAAKLTMNGTSSAGTLAVSHDPVKPVT TSPLPAETDICLFSGSITQVGSPVGSAMNLIPED GLPPILISTGVKGDYAVEEKPSQISVMQQLEDG GPDPLVFVLNANLLSMVKIVNYVNRKCWCFTTK GMHAVGQSEIVILLQCLPDEKCLPKDIFNHFVQL YRDALAGNVVSNLGHSFFSQSFLGSKEHGGFLY VTSTYQSLQDLVLPTPPYLFGILIQKWETPWAK VFPIRLMLRLGAEYRLYPCPLFSVRFRKPLFGET GHTIMNLLADFRNYQYTLPVVQGLVVDMEVRKT SIKIPSNRYNEMMKAMNKSNEHVLAGGACFNEK ADSHLVCVQNDDGNYQTQAISIHNQPRKVTGASF FVFSGALKSSSGYLAKSSIVEDGVMVQITAENM DSLRQALREMKDFTITCGKADAEEPQEHIHIQW VDDDKNVSKGVVSPIDGKSMETITNVKIFHGSEY KANGKVIRWTEVFFLENDDQHNCLSDPADHSRL TEHVAKAFCLALCPHLKLLKEDGMTKLGLRVTL DSDQVGYQAGSNGQPLPSQYMNDLDSALVPVIHG GACQLSEGPVVMELIFYILENIV*TEKTSFFSV QTCCNSSHTQIICTLKLED*AFVNTINGVGNRV GVGVWETGGKGWLGGPMFHNSKSSMHCPPRRSGQ LLFLHNSYAILAANPLLLLFRQEFRSSLKIYLW SCAQKCSNGYNHHQGWDGRAEGK*NIKHQFLHS LYRIGIKRIIPH*

Frame 2

AEAEAAAARGSNRAY*ISRTGWWCSRHHE*APR SLFLITCVRGKRFKQVS*VVFPHRWRITSKQKL TTWTRC*MNLNKTKMKQFLLLYWIQSGIRF*IP LLTGCHLTLLWPV*MNLQFLMSHNHN*KSSPWLI QLP*PQRKRITVLMDRTVI*IQRLPQCGLMKML LQKTS*LRETIVGMINAVLLKWERRNVETWLVC QMRRMFLL*PSCITVIKGHYKTIYRIVIIIIVNP LWMLLAVHWIMKTDKLINLVLV*MSPLKKI*IQ RNKWIH*IDRKQRGDLLTICVLLHLIV*PVSVP LHN*RMTEV*VETPPCLRLQV*RLIQ*SHPREQM DVLLLKSKRTIYQMRTSLAKSALLGQI*GVQIP FPT*VRGF**KKSQQRRAPLKNPSGLVYLCFSN QTCLMGLEGIMTVNGVQIALCLMKLGLMKMKVMN MKKLLALQNSLI*QSISLNLRT*LIGS*LN*MR *MIAK*TKKRKSFYRLVSLRTLMVIVEDSVLDW QMQV*I*KELALVKVKNVISPLL*THQQQIIYLM VVIPMECKTQVFLLFQRLYPPKKIQ*QKKKK*R KASQNATQIFMNREEMRPQKGVDYF*TALVT** RKIIYIISVVKFHQCLGNLPPR**QACHLSVFLL VVQDPSNLLILNFKFQSHYQTIYKMTFLQTVEI ILKIKMIFLGKQN*GKTQQPMYAVHLWETSLMS IQMGNI*KVMRLRSPLDHALH*LQIAQIMISELV SLEFLPESHSPLWVRWLQYGYRILRLQIA*NVK PGLHSPKGGITAEHVGRFSVLPAVA*NVNCYTW TERKLECV*SAIQC**MWLSPESRGEFGLLMGSC PMEKLLMQPN*Q*MELPLQEPWLCHTTQSSQ*L PVLYQQRRIFVYSLGV*LRLEVLLEVQ*ILFLK MAFLPFSSPLV*KETMLWKRNHHRFQ*CSSWRMV ALTHLYLF*MQICCQWLKL*IM*TGSAGVSQPR ECMQWVSLR*SFFYSVYRMKSVCQRISLITLCS FIGMLWQGMW*ATWDIPSSVKVSLAVKNMVDSYM *HLPTSHCKT*YSQPHLTCLGFLSRNGKLLGLK YFLSV*C*DLELNIDFIHAHYSVSDFGSHCLER RGIPS*IFLQTSEITSIPCQ*FKVWWLIWKFGKL ASKFPATDTMR**KP*TSPMSMSWQEVPASMKR QTLILCVYRMMMETIRPRLSVFTISPEK*LVPV SLCSVAL*NPLLDTLPSPVLWKMVLWSRLLQRTW IP*GRHCER*RTSPSPVGRRTRRNPRSTSTSSG WMMTRTLARVS*VL*MGSPWRL*QM*RYSMDQN IKQMEK*SDGQRCFS*KTMTSTIASVILQITVD* LSMLPKLFALLSVLT*NF*RKME*PNWDYV*HL TQIRLAIKQGAMASPFPRST*MIWTAPWCR*SM EGPASLVRAPLSWNSSFIFWKTSYKQRRLHFFLF RLVATAVIPKSFAL*NWKIKLLLTLLMGWGIGW EWGFGRRVGKGGWGDRCSIILSLLCIVHQEDLG SFCSCTTVMLSLQLIPFCYCLDKNSAPLSRFTYG HVLRNAQMGTTITKGGMGGQRGNKI*SISSCTL CTELV*KG*FHT

Sequence NM_004799

ATGCTGAAGCGGAGGCGGCCGCTGCTCGAGGATCAAACAGAGCATACTGAATCAGCAGGACTGGCTGGTGGTGCAGCAGACATCATGAGTAAGCACCGAG AAGTCTGTTCCTTATCACGTGTGTAAGGGGAAAAAGGTTTAAACAAGTCTCTTAAGTGGTGTTTCCTCACCGATGGAGAATTACTTCCAAGCAGAAGCTT ACAACCTGGACAAGGTGTTAGATGAATTTGAACAAAACGAAGATGAAACAGTTTCTTCTACTTTATTGGATACAAAGTGGAATAAGATTCTAGATCCCCC TTCTCACCGGCTGTCATTTAACCCTACTTTGGCCAGTGTGAATGAATCTGCAGTTTCTAATGAGTCACAACCACAACTGAAAGTCTTCTCCCTGGCTCAT TCAGCTCCCCTGACCACAGAGGAAGAGGATCACTGTGCTAATGGACAGGACTGTAATCTAAATCCAGAGATTGCCACAATGTGGATTGATGAAAATGCTG TTGCAGAAGACCAGTTAATTAAGAGAAACTATAGTTGGGATGATCAATGCAGTGCTGTTGAAGTGGGAGAGAAGAAATGTGGAAACCTGGCTTGTCTGCC AGATGAGAAGAATGTTCTTGTTGTAGCCGTCATGCATAACTGTGATAAAAGGACATTACAAAACGATTTACAGGATTGTAATAATTATAATAGTCAATCC CTTATGGATGCTTTTAGCTGTTCACTGGATAATGAAAACAGACAAACTGATCAATTTAGTTTTAGTATAAATGAGTCCACTGAAAAAGATATGAATTCAG AGAAACAAATGGATCCATTGAATAGACCGAAAACAGAGGGGAGATCTGTTAACCATCTGTGTCCTACTTCATCTGATAGTCTAGCCAGTGTCTGTTCCCC TTCACAATTAAAGGATGACGGAAGTATAGGTAGAGACCCCTCCATGTCTGCGATTACAAGTTTAACGGTTGATTCAGTAATCTCATCCCAGGGAACAGAT GGATGTCCTGCTGTTAAAAAGCAAGAGAACTATATACCAGATGAGGACCTCACTGGCAAAATCAGCTCTCCTAGGACAGATCTAGGGAGTCCAAATTCCT TTTCCCACATGAGTGAGGGGATTTTGATGAAAAAAGAGCCAGCAGAGGAGAGCACCACTGAAGAATCCCTCCGGTCTGGTTTACCTTTGCTTCTCAAACC AGACATGCCTAATGGGTCTGGAAGGAATAATGACTGTGAACGGTGTTCAGATTGCCTTGTGCCTAATGAAGTTAGGGCTGATGAAAATGAAGGTTATGAA CATGAAGAAACTCTTGGCACTACAGAATTCCTTAATATGACAGAGCATTTCTCTGAATCTCAGGACATGACTAATTGGAAGTTGACTAAACTAAATGAGA TGAATGATAGCCAAGTAAACGAAGAAAAGGAAAAGTTTCTACAGATTAGTCAGCCTGAGGACACTAATGGTGATAGTGGAGGACAGTGTGTTGGATTGGC AGATGCAGGTCTAGATTTAAAAGGAACTTGCATTAGTGAAAGTGAAGAATGTGATTTCTCCACTGTTATAGACACACCAGCAGCAAATTATCTATCTAAT GGTTGTGATTCCTATGGAATGCAAGACCCAGGTGTTTCTTTTGTTCCAAAGACTTTACCCTCCAAAGAAGATTCAGTAACAGAAGAAAAAGAAATAGAGG AAAGCAAGTCAGAATGCTACTCAAATATTTATGAACAGAGAGGAAATGAGGCCACAGAAGGGAGTGGACTACTTTTAAACAGCACTGGTGACCTAATGAA GAAAAATTATTTACATAATTTCTGTAGTCAAGTTCCATCAGTGCTTGGGCAATCTTCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTT GGTGGTGCAAGACCCAAGCAACCTTCTAATCTTAAACTTCAAATTCCAAAGCCATTATCAGACCATTTACAAAATGACTTTCCTGCAAACAGTGGAAATA ATACTAAAAATAAAAATGATATTCTTGGGAAAGCAAAATTAGGGGAAAACTCAGCAACCAATGTATGCAGTCCATCTTTGGGAAACATCTCTAATGTCGA TACAAATGGGGAACATTTAGAAAGTTATGAGGCTGAGATCTCCACTAGACCATGCCTTGCATTAGCTCCAGATAGCCCAGATAATGATCTCAGAGCTGGT CAGTTTGGAATTTCTGCCAGAAAGCCATTCACCACTCTGGGTGAGGTGGCTCCAGTATGGGTACCGGATTCTCAGGCTCCAAATTGCATGAAATGTGAAG CCAGGTTTACATTCACCAAAAGGAGGCATCACTGCAGAGCATGTGGGAAGGTTTTCTGTGCTTCCTGCTGTAGCCTGAAATGTAAACTGTTATACATGGA CAGAAAGGAAGCTAGAGTGTGTGTAATCTGCCATTCAGTGCTAATGAATGCTCAAGCCTGGGAGAACATGATGAGTGCCTCAAGCCAGAGCCCTAACCCT AACAATCCTGCTGAATACTGTTCTACTATCCCTCCCTTGCAGCAAGCTCAGGCCTCAGGAGCTCTGAGCTCTCCACCTCCCACTGTGATGGTACCTGTGG GAGTTTTAAAGCACCCTGGAGCAGAAGTGGCTCAGCCCAGAGAGCAGAGGCGAGTTTGGTTTGCTGATGGGATCTTGCCCAATGGAGAAGTTGCTGATGC AGCCAAATTAACAATGAATGGAACTTCCTCTGCAGGAACCCTGGCTGTGTCACACGACCCAGTCAAGCCAGTAACTACCAGTCCTCTACCAGCAGAGACG GATATTTGTCTATTCTCTGGGAGTATAACTCAGGTTGGAAGTCCTGTTGGAAGTGCAATGAATCTTATTCCTGAAGATGGCCTTCCTCCCATTCTCATCT CCACTGGTGTAAAAGGAGACTATGCTGTGGAAGAGAAACCATCACAGATTTCAGTAATGCAGCAGTTGGAGGATGGTGGCCCTGACCCACTTGTATTTGT TTTAAATGCAAATTTGTTGTCAATGGTTAAAATTGTAAATTATGTGAACAGGAAGTGCTGGTGTTTCACAACCAAGGGAATGCATGCAGTGGGTCAGTCT GAGATAGTCATTCTTCTACAGTGTTTACCGGATGAAAAGTGTTTGCCAAAGGATATCTTTAATCACTTTGTGCAGCTTTATCGGGATGCTCTGGCAGGGA ATGTGGTGAGCAACTTGGGACATTCCTTCTTCAGTCAAAGTTTCCTTGGCAGTAAAGAACATGGTGGATTCTTATATGTGACATCTACCTACCAGTCACT GCAAGACCTAGTACTCCCAACCCCACCTTACTTGTTTGGGATTCTTATCCAGAAATGGGAAACTCCTTGGGCTAAAGTATTTCCTATCCGTCTGATGTTG AGACTTGGAGCTGAATATCGACTTTATCCATGCCCACTATTCAGTGTCAGATTTCGGAAGCCATTGTTTGGAGAGACGGGGCATACCATCATGAATCTTC TTGCAGACTTCAGAAATTACCAGTATACCTTGCCAGTAGTTCAAGGTTTGGTGGTTGATATGGAAGTTCGGAAAACTAGCATCAAAATTCCCAGCAACAG ATACAATGAGATGATGAAAGCCATGAACAAGTCCAATGAGCATGTCCTGGCAGGAGGTGCCTGCTTCAATGAAAAGGCAGACTCTCATCTTGTGTGTGTA CAGAATGATGATGGAAACTATCAGACCCAGGCTATCAGTATTCACAATCAGCCCAGAAAAGTGACTGGTGCCAGTTTCTTTGTGTTCAGTGGCGCTCTGA AATCCTCTTCTGGATACCTTGCCAAGTCCAGTATTGTGGAAGATGGTGTTATGGTCCAGATTACTGCAGAGAACATGGATTCCTTGAGGCAGGCACTGCG AGAGATGAAGGACTTCACCATCACCTGTGGGAAGGCGGACGCGGAGGAACCCCAGGAGCACATCCACATCCAGTGGGTGGATGATGACAAGAACGTTAGC AAGGGTGTCGTAAGTCCTATAGATGGGAAGTCCATGGAGACTATAACAAATGTGAAGATATTCCATGGATCAGAATATAAAGCAAATGGAAAAGTAATCA GATGGACAGAGGTGTTTTTCCTAGAAAACGATGACCAGCACAATTGCCTCAGTGATCCTGCAGATCACAGTAGATTGACTGAGCATGTTGCCAAAGCTTT TTGCCTTGCTCTCTGTCCTCACCTGAAACTTCTGAAGGAAGATGGAATGACCAAACTGGGACTACGTGTGACACTTGACTCAGATCAGGTTGGCTATCAA GCAGGGAGCAATGGCCAGCCCCTTCCCTCGCAGTACATGAATGATCTGGACAGCGCCTTGGTGCCGGTGATCCATGGAGGGGCCTGCCAGCTTAGTGAGG GCCCCGTTGTCATGGAACTCATCTTTTATATTCTGGAAAACATCGTATAAACAGAGAAGACTTCATTTTTTTCTGTTCAGACTTGTTGCAACAGCAGTCA TACCCAAATCATTTGCACTTTAAAACTGGAAGATTAAGCTTTTGTTAACACTATTAATGGGGTGGGGAATAGGGTGGGAGTGGGGGTTTGGGAGACGGGT GGGAAAGGGTGGTTGGGGGGACCGATGTTCCATAATTCTAAGTCTTCTATGCATTGTCCACCAAGAAGATCTGGGCAGCTTCTGTTCCTGCACAACAGTT ATGCTATCCTTGCAGCTAATCCCCTTCTGTTACTGTTTAGACAAGAATTCCGCTCCTCTCTCAAGATTTACTTATGGTCATGTGCTCAGAAATGCTCAAA TGGGTACAACCATCACCAAGGGTGGGATGGGAGGGCAGAGGGGAAATAAAATATAAAGCATCAGTTCTTGCACTCTTTGTACAGAATTGGTATAAAAAGG ATAATTCCACACTGA

refseq_ZFYVE9.F2 (forward primer)
refseq_ZFYVE9.R2 (reverse primer)
Single exon skipping

Frame 0

MLKRRRPLLEDQTEHTESAGLAGGAADIMSKHR EVCSLSRV*GEKGLNKSLKWCFLTDGELLPSRSL QPGQGVR*I*TKRR*NSFFYFIGYKVE*DSRSP FSPAVI*PYFGQCE*ICSF**VTTTTESLLPGS FSSPDHRGRGSLC*WTGL*SKSRDCHNVD**KCC CRRPVN*EKL*LG*SMQCC*SGREEMWKPGLSA R*EECSCCSRHA*L**KDITKRFTGL**L**SI PYGCF*LFTG**KQTN*SI*F*YK*VH*KRYEFR ETNGSIE*TENRGEIC*PSVSYFI**SSQCLFP FTIKG*RKYR*RPLHVCDYKFNG*FSNLIPGNR WMSCC*KARELYTR*GPHWQNQLS*DRSRESKFL FPHE*GDFDEKRASRGEHH*RIPPVWFTFASQT RHA*WVWKE**L*TVFRLPCA**S*G**K*RL* T*RNSWHYRIP*YDRAFL*ISGHD*LEVD*TK*D E**PSKRRKGKVSTD*SA*GH*W**WRTVCWIG RCRSRFKRNLH**K*RM*FLHCYRHTSSKLSI* WL*FLWNARPRCFFCSKDFTLQRRFSNRRKRNRG KQVRMLLKYL*TERK*GHRREWTTFKQHW*PNE EKLFT*FL*SSSISAWAIFPQGSSKPAIYQCSF WWCKTQATF*S*TSNSKAIIRPFTK*LSCKQWK* Y*K*K*YSWESKIRGKLSNQCMQSIFGKHL*CR YKWGTFRKL*G*DLH*TMPCISSR*PR**SQSW SVWNFCQKAIHHSG*GGSSMGTGFSGSKLHEM*S QVYIHQKEASLQSMWEGFLCFLL*PEM*TVIHG QKGS*SVCNLPFSANECSSLGEHDECLKPEP*P *QSC*ILFYYPSLAASSGLRSSELSTSHCDGTCG SFKAPWSRSGSAQRAEASLVC*WDLAQWRSC*C SQINNEWNFLCRNPGCVTRPSQASNYQSSTSRD GYLSILWEYNSGWKSCWKCNESYS*RWPSSHSHL HWCKRRLCCGRETITDFSNAAVGGWWP*PTCIC FKCKFVVNG*NCKLCEQEVLVFHNQGNACSGSV *DSHSSTVFTG*KVFAKGYL*SLCAALSGCSGRE CGEQLGTFLLQSKFPWQ*RTWWILICDIYLPVT ARPSTPNPTLLVWDSYPEMGNSLG*SISYPSDV ETWS*ISTLSMPTIQCQISEAIVWRDGAYHHESS CRLQKLPVYLASSSRFGG*YGSSEN*HQNSQQQ IQ*DDESHEQVQ*ACPGRRCLLQ*KGRLSSCVC TE**WKLSDPGYQYSQSAQKSDWCQFLCVQWRSE ILFWIPCQVQYCGRWCYGPDYCREHGFLEAGTA RDEGLHHHLWEGGRGGTPGAHPHPVGG**QER* QGCRKSYRWEVHGDYNKCEDIPWIRI*SKWKSNQ MDRGVFPRKR*PAQLPQ*SCRSQ*ID*ACCQSF LPCSLSSPETSEGRWNDQTGTTCDT*LRSGWLS SREQWPAPSLAVHE*SGQRLGAGDPWRGLPA**G PRCHGTHLLYSGKHRINREDFIFFCSDLLQQQS YPNHLHFKTGRLSFC*HY*WGGE*GGSGGLGDG WERVVGGTDVP*F*VFYALSTKKIWAASVPAQQL CYPCS*SPSVTV*TRIPLLSQDLLMVMCSEMLK WVQPSPRVGWEGRGEIKYKASVLALFVQNWYKK DNSTL

Frame 1

C*SGGGRCSRIKQSILNQQDWLVVQQTS*VSTE KSVPYHVCKGKKV*TSLLSGVSSPMENYFQAEA YNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPP SHRLSFNPTLASVNESAVSNESQPQLKVFSLAH SAPLTTEEEDHCANGQDCNLNPEIATMWIDENA VAEDQLIKRNYSWDDQCSAVEVGEKKCGNLACLP DEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQS LMDAFSCSLDNENRQTDQFSFSINESTEKDMNS EKQMDPLNRPKTEGRSVNHLCPTSSDSLASVCSP SQLKDDGSIGRDPSMSAITSLTVDSVISSQGTD GCPAVKKQENYIPDEDLTGKISSPRTDLGSPNS FSHMSEGILMKKEPAEESTTEESLRSGLPLLLKP DMPNGSGRNNDCERCSDCLVPNEVRADENEGYE HEETLGTTEFLNMTEHFSESQDMTNWKLTKLNE MNDSQVNEEKEKFLQISQPEDTNGDSGGQCVGLA DAGLDLKGTCISESEECDFSTVIDTPAANYLSN GCDSYGMQDPGVSFVPKTLPSKEDSVTEEKEIE ESKSECYSNIYEQRGNEATEGSGLLLNSTGDLMK KNYLHNFCSQVPSVLGQSSPKVVASLPSISVPF GGARPKQPSNLKLQIPKPLSDHLQNDFPANSGN NTKNKNDILGKAKLGENSATNVCSPSLGNISNVD TNGEHLESYEAEISTRPCLALAPDSPDNDLRAG QFGISARKPFTTLGEVAPVWVPDSQAPNCMKCE ARFTFTKRRHHCRACGKVFCASCCSLKCKLLYMD RKEARVCVICHSVLMNAQAWENMMSASSQSPNP NNPAEYCSTIPPLQQAQASGALSSPPPTVMVPV GVLKHPGAEVAQPREQRRVWFADGILPNGEVADA AKLTMNGTSSAGTLAVSHDPVKPVTTSPLPAET DICLFSGSITQVGSPVGSAMNLIPEDGLPPILI STGVKGDYAVEEKPSQISVMQQLEDGGPDPLVFV LNANLLSMVKIVNYVNRKCWCFTTKGMHAVGQS EIVILLQCLPDEKCLPKDIFNHFVQLYRDALAG NVVSNLGHSFFSQSFLGSKEHGGFLYVTSTYQSL QDLVLPTPPYLFGILIQKWETPWAKVFPIRLML RLGAEYRLYPCPLFSVRFRKPLFGETGHTIMNL LADFRNYQYTLPVVQGLVVDMEVRKTSIKIPSNR YNEMMKAMNKSNEHVLAGGACFNEKADSHLVCV QNDDGNYQTQAISIHNQPRKVTGASFFVFSGAL KSSSGYLAKSSIVEDGVMVQITAENMDSLRQALR EMKDFTITCGKADAEEPQEHIHIQWVDDDKNVS KGVVSPIDGKSMETITNVKIFHGSEYKANGKVI RWTEVFFLENDDQHNCLSDPADHSRLTEHVAKAF CLALCPHLKLLKEDGMTKLGLRVTLDSDQVGYQ AGSNGQPLPSQYMNDLDSALVPVIHGGACQLSE GPVVMELIFYILENIV*TEKTSFFSVQTCCNSSH TQIICTLKLED*AFVNTINGVGNRVGVGVWETG GKGWLGGPMFHNSKSSMHCPPRRSGQLLFLHNS YAILAANPLLLLFRQEFRSSLKIYLWSCAQKCSN GYNHHQGWDGRAEGK*NIKHQFLHSLYRIGIKR IIPH*

Frame 2

AEAEAAAARGSNRAY*ISRTGWWCSRHHE*APR SLFLITCVRGKRFKQVS*VVFPHRWRITSKQKL TTWTRC*MNLNKTKMKQFLLLYWIQSGIRF*IP LLTGCHLTLLWPV*MNLQFLMSHNHN*KSSPWLI QLP*PQRKRITVLMDRTVI*IQRLPQCGLMKML LQKTS*LRETIVGMINAVLLKWERRNVETWLVC QMRRMFLL*PSCITVIKGHYKTIYRIVIIIIVNP LWMLLAVHWIMKTDKLINLVLV*MSPLKKI*IQ RNKWIH*IDRKQRGDLLTICVLLHLIV*PVSVP LHN*RMTEV*VETPPCLRLQV*RLIQ*SHPREQM DVLLLKSKRTIYQMRTSLAKSALLGQI*GVQIP FPT*VRGF**KKSQQRRAPLKNPSGLVYLCFSN QTCLMGLEGIMTVNGVQIALCLMKLGLMKMKVMN MKKLLALQNSLI*QSISLNLRT*LIGS*LN*MR *MIAK*TKKRKSFYRLVSLRTLMVIVEDSVLDW QMQV*I*KELALVKVKNVISPLL*THQQQIIYLM VVIPMECKTQVFLLFQRLYPPKKIQ*QKKKK*R KASQNATQIFMNREEMRPQKGVDYF*TALVT** RKIIYIISVVKFHQCLGNLPPR**QACHLSVFLL VVQDPSNLLILNFKFQSHYQTIYKMTFLQTVEI ILKIKMIFLGKQN*GKTQQPMYAVHLWETSLMS IQMGNI*KVMRLRSPLDHALH*LQIAQIMISELV SLEFLPESHSPLWVRWLQYGYRILRLQIA*NVK PGLHSPKGGITAEHVGRFSVLPAVA*NVNCYTW TERKLECV*SAIQC**MLKPGRT**VPQARALTL TILLNTVLLSLPCSKLRPQEL*ALHLPL*WYLW EF*STLEQKWLSPESRGEFGLLMGSCPMEKLLM QPN*Q*MELPLQEPWLCHTTQSSQ*LPVLYQQRR IFVYSLGV*LRLEVLLEVQ*ILFLKMAFLPFSS PLV*KETMLWKRNHHRFQ*CSSWRMVALTHLYL F*MQICCQWLKL*IM*TGSAGVSQPRECMQWVSL R*SFFYSVYRMKSVCQRISLITLCSFIGMLWQG MW*ATWDIPSSVKVSLAVKNMVDSYM*HLPTSH CKT*YSQPHLTCLGFLSRNGKLLGLKYFLSV*C* DLELNIDFIHAHYSVSDFGSHCLERRGIPS*IF LQTSEITSIPCQ*FKVWWLIWKFGKLASKFPAT DTMR**KP*TSPMSMSWQEVPASMKRQTLILCVY RMMMETIRPRLSVFTISPEK*LVPVSLCSVAL* NPLLDTLPSPVLWKMVLWSRLLQRTWIP*GRHC ER*RTSPSPVGRRTRRNPRSTSTSSGWMMTRTLA RVS*VL*MGSPWRL*QM*RYSMDQNIKQMEK*S DGQRCFS*KTMTSTIASVILQITVD*LSMLPKL FALLSVLT*NF*RKME*PNWDYV*HLTQIRLAIK QGAMASPFPRST*MIWTAPWCR*SMEGPASLVR APLSWNSSFIFWKTSYKQRRLHFFLFRLVATAV IPKSFAL*NWKIKLLLTLLMGWGIGWEWGFGRRV GKGGWGDRCSIILSLLCIVHQEDLGSFCSCTTV MLSLQLIPFCYCLDKNSAPLSRFTYGHVLRNAQ MGTTITKGGMGGQRGNKI*SISSCTLCTELV*KG *FHT