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Sequences

Sequence NM_197968

AGCGCGCGGAGGGCGGGGCCTCCCCGGGCTCGGCCGCGCCGCCGCCTCCTCCGCCTCCTCCTCCTCACCAGCGCTAAAGCCGGGACTGGACCGAGCGGAG
TTGTGCGTGTCGCCGAAGGGGGGTGGGCCGGGGGAGGGGAGGTTCGTTCCGCGGAGCCCCAGCCAGAACCGGGACCAAGAATCGCCTTCAGCCCTGTCTG
GTGCATCCTTGGCAGAAAGTGAGGAGGAAAACACCCCCATTGTTCTTTGGCATGGACACAAGTTCAGTGGGAGGATTAGAATTGACTGATCAGACTCCTG
TTTTATTAGGGAGTACGGCCATGGCAACTAGTCTCACGAATGTAGGAAACTCATTTAGTGGTCCAGCTAATCCTTTAGTGTCTAGATCTAATAAGTTTCA
GAACTCGTCAGTGGAAGATGATGATGATGTTGTTTTTATCGAACCTGTACAACCTCCCCCACCTTCTGTACCAGTGGTAGCTGATCAAAGAACCATAACA
TTTACATCATCAAAAAATGAAGAACTACAAGGAAATGATTCCAAAATTACTCCTTCCTCAAAAGAGTTGGCATCTCAGAAGGGAAGTGTAAGTGAGACAA
TTGTCATTGATGATGAAGAGGACATGGAAACAAATCAAGGGCAAGAGAAAAATTCCTCCAATTTTATTGAACGAAGACCTCCTGAGACTAAAAACAGAAC
CAATGATGTGGATTTCTCCACTTCCAGTTTTTCAAGAAGTAAGGTAAATGCAGGAATGGGTAATAGTGGTATCACCACAGAACCAGACTCTGAAATTCAG
ATTGCTAATGTTACAACTTTAGAAACAGGTGTAAGCTCTGTGAATGATGGCCAATTAGAAAATACTGACGGGCGAGATATGAACTTAATGATTACACATG
TAACATCACTGCAGAATACCAACTTGGGAGATGTCTCTAACGGACTGCAGTCAAGTAATTTTGGTGTTAATATACAAACATACACCCCATCTTTAACTTC
ACAGACCAAGACTGGAGTAGGACCTTTTAATCCTGGTAGAATGAATGTGGCAGGAGACGTTTTTCAGAATGGAGAATCTGCAACTCATCATAATCCTGAT
TCTTGGATCTCCCAGTCAGCTTCATTTCCCCGTAATCAGAAACAACCAGGGGTGGACTCTTTATCACCAGTGGCCTCACTTCCTAAACAGATTTTCCAGC
CGTCTGTCCAACAGCAGCCTACTAAACCAGTTAAAGTCACTTGTGCAAACTGCAAAAAACCTTTACAGAAGGGCCAGACAGCTTATCAACGAAAAGGATC
AGCTCACCTCTTTTGTTCTACCACCTGCCTTTCTTCCTTCTCCCACAAGCCTGCTCCAAAGAAACTCTGTGTTATGTGTAAAAAAGATATAACTACAATG
AAAGGAACCATTGTTGCTCAAGTGGATTCAAGTGAGTCCTTCCAGGAATTCTGTAGTACATCTTGTTTATCTCTCTATGAAGACAAACAGAATCCTACTA
AAGGAGCTCTAAATAAATCAAGATGTACAATCTGTGGTAAACTAACTGAGATTCGCCATGAAGTCAGCTTTAAAAATATGACTCATAAGCTGTGCAGTGA
CCACTGCTTTAATAGATATAGAATGGCCAATGGTTTAATAATGAATTGCTGTGAACAGTGTGGAGAGTACTTGCCCAGTAAAGGTGCTGGAAATAATGTT
CTGGTGATTGATGGTCAACAGAAAAGATTTTGCTGTCAAAGTTGTGTCAGTGAATACAAACAGGTAGGTAGCCATCCAAGCTTCCTGAAGGAGGTTCGAG
ATCACATGCAGGACTCTTTCTTAATGCAGCCTGAGAAATATGGAAAACTGACAACTTGTACTGGTTGCCGAACACAGTGCAGGTTTTTTGATATGACTCA
GTGTATAGGTCCTAATGGATATATGGAGCCATATTGTTCAACTGCTTGTATGAACAGTCACAAGACAAAATATGCAAAATCACAAAGTTTGGGAATTATT
TGCCATTTTTGTAAGCGAAACTCTTTACCTCAATACCAAGCCACAATGCCTGATGGAAAACTGTACAACTTTTGCAATTCCAGTTGTGTGGCTAAATTTC
AGGCTCTAAGTATGCAGTCATCTCCAAATGGCCAGTTTGTAGCGCCAAGTGATATTCAGTTGAAATGCAACTACTGCAAAAATTCCTTTTGTTCAAAACC
AGAAATCCTGGAATGGGAGAACAAAGTGCATCAGTTCTGCAGCAAAACTTGTTCAGATGACTATAAGAAGTTGCATTGCATAGTTACATATTGCGAATAC
TGTCAAGAGGAGAAGACTCTTCATGAAACAGTAAATTTCTCTGGCGTTAAGAGACCTTTCTGTAGTGAAGGCTGCAAATTATTATACAAACAGGATTTTG
CCAGACGTTTAGGATTGAGATGTGTTACTTGCAACTATTGTTCTCAGCTATGTAAGAAGGGAGCAACTAAAGAACTCGATGGTGTTGTGAGAGATTTCTG
CAGTGAAGATTGCTGTAAAAAATTTCAGGATTGGTACTACAAGGCTGCAAGGTGTGACTGTTGTAAATCTCAAGGAACTCTTAAAGAGCGAGTTCAGTGG
CGTGGGGAAATGAAACATTTCTGTGATCAACATTGCTTACTGCGTTTCTACTGTCAACAAAATGAGCCCAACATGACAACTCAGAAAGGACCTGAAAACT
TACATTATGATCAGGGTTGTCAGACATCTCGAACCAAAATGACAGGTTCAGCACCACCCCCTTCTCCAACACCTAACAAAGAGATGAAGAACAAAGCAGT
TCTTTGCAAACCTTTAACAATGACAAAAGCTACTTACTGTAAACCTCACATGCAGACCAAATCTTGTCAGACAGATGATACTTGGAGGACAGAATATGTT
CCAGTGCCTATCCCTGTGCCTGTGTATATCCCAGTTCCTATGCACATGTACAGTCAGAATATTCCTGTTCCTACTACAGTTCCTGTTCCTGTGCCAGTTC
CTGTTTTTCTGCCTGCTCCATTGGACAGCAGTGAGAAGATTCCTGCAGCAATTGAGGAGCTAAAAAGCAAGGTTTCTTCAGATGCTCTTGATACAGAGTT
GCTTACAATGACGGATATGATGAGTGAAGACGAGGGGAAAACAGAGACAACCAACATCAACAGTGTAATTATTGAAACAGATATAATTGGTTCAGACCTT
TTGAAGAACTCTGACCCAGAGACACAGTCCAGCATGCCTGATGTACCATATGAACCAGATTTGGATATCGAAATAGATTTTCCCAGAGCTGCTGAGGAGC
TTGATATGGAAAATGAATTTTTATTACCACCTGTTTTTGGCGAAGAATATGAGGAACAGCCCAGACCTCGATCTAAAAAAAAGGGAGCCAAGAGAAAGGC
TGTATCAGGATACCAGTCTCATGATGATAGTTCTGACAATTCAGAATGCAGCTTTCCTTTCAAATATACGTATGGCGTAAATGCATGGAAACACTGGGTC
AAAACTAGGCAACTTGATGAAGATCTTCTGGTATTAGATGAGTTAAAATCTTCTAAATCAGTAAAGTTAAAAGAGGATCTACTCTCTCACACCACAGCTG
AGCTTAACTATGGGTTAGCTCATTTTGTCAATGAGATCCGACGGCCAAATGGAGAGAATTATGCACCTGACAGCATCTATTACCTTTGCCTTGGAATACA
GGAGTATTTGTGTGGAAGTAATCGAAAAGACAACATATTTATTGATCCTGGATACCAAACATTTGAGCAAGAATTGAATAAAATACTGCGAAGCTGGCAA
CCAAGCATACTTCCAGATGGGTCAATATTCTCTCGAGTTGAAGAAGACTATCTCTGGAGGATAAAACAACTAGGATCACACTCTCCAGTAGCTCTTCTGA
ATACACTGTTCTACTTTAACACTAAGTATTTTGGCCTGAAAACAGTGGAACAACACTTAAGACTTTCCTTTGGCACTGTGTTTAGGCATTGGAAAAAAAA
TCCTTTAACGATGGAAAACAAAGCGTGTCTTCGATACCAAGTGTCTTCCTTGTGTGGAACAGATAATGAAGATAAAATTACTACTGGAAAAAGAAAACAT
GAAGATGATGAGCCAGTATTTGAACAAATTGAAAACACAGCCAATCCTTCCAGATGTCCTGTGAAAATGTTTGAATGCTACTTGTCTAAAAGTCCACAGA
ATCTTAATCAGAGGATGGATGTTTTTTATTTGCAACCAGAATGCTCTAGTTCTACAGATAGCCCTGTCTGGTATACGTCTACTTCACTGGACCGAAACAC
CTTGGAAAATATGCTTGTACGGGTTCTTCTAGTAAAAGATATTTATGATAAAGACAATTATGAACTGGATGAAGACACAGACTAAAAAGGAACGTTGCAG
AAGCAATCGGGATAAAACAGCATTAGATAGTCATGCTGCTAGATCTTTATTATGGAAAACATTTCAAGTTTACTCCTTCTGTTTTGAGTTTTGTAGCAGT
GTACCCACGCTGGGTATTACCATGTAAATAATCTGTGAGTGAAAGTTGCCATTATTCTATGTAGTGGTTTTAGGATACTTAACAAATACATTCAAATTCT
TTTTTTATTATTATTTATTTGATTAGGTATGTTTGTAACTTTTTACATTACAGAATATGAATGAGAATGTGCCATGTATAATTTTTTTCTTGTAGTAAGA
AACATCCATATTGCACAACTCTACTGTTGCAAAGCTTCCTTGGAAGGGGGCTCTTTTACTGGGTTCTTAACCAGATGGTTGTGTATGGGTAGCACTACTA
AAAGTTTAGAACTTGCAGTGTCTTTCGGAATTTTTAAAATAAACTGTAAACTAATAGGCTGGGGTTTTTGTTTTGTTTTGGGGTTTTGTTTTGTTTGGTT
TTACATTTTAGTTACTGAAGCCTTACAAGGTTATGTAGAGAGATACCATCTTCTGTACCAAAAATAGACAAGAGAATGCTGTCAATATTGGTGTACTGTA
ATGTGAATCTATGCTGGTGAAAACAATTTTTTGTTCCCCTTATTAAAACCTTAGTGTCCTTTTCTCATTTGTGGCTTTCTGCATCACCCAATCAATAAAA
AACAAATATATATATGTATATATATGTATGTATGTGTGTGTGTATATATATATATATATATATATATATA
  • refseq_ZMYM2.F2 (forward primer)
  • refseq_ZMYM2.R2 (reverse primer)
  • Single exon skipping

Frame 0

SARRAGPPRARPRRRLLRLLLLTSAKAGTGPSG
VVRVAEGGWAGGGEVRSAEPQPEPGPRIAFSPVW
CILGRK*GGKHPHCSLAWTQVQWED*N*LIRLL
FY*GVRPWQLVSRM*ETHLVVQLIL*CLDLISF
RTRQWKMMMMLFLSNLYNLPHLLYQW*LIKEP*H
LHHQKMKNYKEMIPKLLLPQKSWHLRREV*VRQ
LSLMMKRTWKQIKGKRKIPPILLNEDLLRLKTE
PMMWISPLPVFQEVR*MQEWVIVVSPQNQTLKFR
LLMLQL*KQV*AL*MMAN*KILTGEI*T**LHM
*HHCRIPTWEMSLTDCSQVILVLIYKHTPHL*L
HRPRLE*DLLILVE*MWQETFFRMENLQLIIILI
LGSPSQLHFPVIRNNQGWTLYHQWPHFLNRFSS
RLSNSSLLNQLKSLVQTAKNLYRRARQLINEKD
QLTSFVLPPAFLPSPTSLLQRNSVLCVKKI*LQ*
KEPLLLKWIQVSPSRNSVVHLVYLSMKTNRILL
KEL*INQDVQSVVN*LRFAMKSALKI*LISCAV
TTALIDIEWPMV***IAVNSVESTCPVKVLEIMF
W*LMVNRKDFAVKVVSVNTNR*VAIQAS*RRFE
ITCRTLS*CSLRNMEN*QLVLVAEHSAGFLI*L
SV*VLMDIWSHIVQLLV*TVTRQNMQNHKVWELF
AIFVSETLYLNTKPQCLMENCTTFAIPVVWLNF
RL*VCSHLQMASL*RQVIFS*NATTAKIPFVQN
QKSWNGRTKCISSAAKLVQMTIRSCIA*LHIANT
VKRRRLFMKQ*ISLALRDLSVVKAANYYTNRIL
PDV*D*DVLLATIVLSYVRREQLKNSMVL*EIS
AVKIAVKNFRIGTTRLQGVTVVNLKELLKSEFSG
VGK*NISVINIAYCVSTVNKMSPT*QLRKDLKT
YIMIRVVRHLEPK*QVQHHPLLQHLTKR*RTKQ
FFANL*Q*QKLLTVNLTCRPNLVRQMILGGQNMF
QCLSLCLCISQFLCTCTVRIFLFLLQFLFLCQF
LFFCLLHWTAVRRFLQQLRS*KARFLQMLLIQS
CLQ*RI**VKTRGKQRQPTSTV*LLKQI*LVQTF
*RTLTQRHSPACLMYHMNQIWISK*IFPELLRS
LIWKMNFYYHLFLAKNMRNSPDLDLKKREPRER
LYQDTSLMMIVLTIQNAAFLSNIRMA*MHGNTGS
KLGNLMKIFWY*MS*NLLNQ*S*KRIYSLTPQL
SLTMG*LILSMRSDGQMERIMHLTASITFALEY
RSICVEVIEKTTYLLILDTKHLSKN*IKYCEAGN
QAYFQMGQYSLELKKTISGG*NN*DHTLQ*LF*
IHCSTLTLSILA*KQWNNT*DFPLALCLGIGKK
IL*RWKTKRVFDTKCLPCVEQIMKIKLLLEKENM
KMMSQYLNKLKTQPILPDVL*KCLNATCLKVHR
ILIRGWMFFICNQNALVLQIALSGIRLLHWTET
PWKICLYGFF**KIFMIKTIMNWMKTQTKKERCR
SNRDKTALDSHAARSLLWKTFQVYSFCFEFCSS
VPTLGITM*IICE*KLPLFYVVVLGYLTNTFKF
FFYYYLFD*VCL*LFTLQNMNENVPCIIFFL**E
TSILHNSTVAKLPWKGALLLGS*PDGCVWVALL
KV*NLQCLSEFLK*TVN**AGVFVLFWGFVLFG
FTF*LLKPYKVM*RDTIFCTKNRQENAVNIGVL*
CESMLVKTIFCSPY*NLSVLFSFVAFCITQSIK
NKYIYVYICMYVCVYIYIYIYIY

Frame 1

ARGGRGLPGLGRAAASSASSSSPALKPGLDRAE
LCVSPKGGGPGEGRFVPRSPSQNRDQESPSALS
GASLAESEEENTPIVLWHGHKFSGRIRID*SDSC
FIREYGHGN*SHECRKLI*WSS*SFSV*I**VS
ELVSGR***CCFYRTCTTSPTFCTSGS*SKNHN
IYIIKK*RTTRK*FQNYSFLKRVGISEGKCK*DN
CH***RGHGNKSRAREKFLQFY*TKTS*D*KQN
Q*CGFLHFQFFKK*GKCRNG**WYHHRTRL*NS
DC*CYNFRNRCKLCE*WPIRKY*RARYELNDYTC
NITAEYQLGRCL*RTAVK*FWC*YTNIHPIFNF
TDQDWSRTF*SW*NECGRRRFSEWRICNSS*S*
FLDLPVSFISP*SETTRGGLFITSGLTS*TDFPA
VCPTAAY*TS*SHLCKLQKTFTEGPDSLSTKRI
SSPLLFYHLPFFLLPQACSKETLCYV*KRYNYN
ERNHCCSSGFK*VLPGIL*YILFISL*RQTESY*
RSSK*IKMYNLW*TN*DSP*SQL*KYDS*AVQ*
PLL**I*NGQWFNNELL*TVWRVLAQ*RCWK*C
SGD*WSTEKILLSKLCQ*IQTGR*PSKLPEGGSR
SHAGLFLNAA*EIWKTDNLYWLPNTVQVF*YDS
VYRS*WIYGAILFNCLYEQSQDKICKITKFGNY
LPFL*AKLFTSIPSHNA*WKTVQLLQFQLCG*IS
GSKYAVISKWPVCSAK*YSVEMQLLQKFLLFKT
RNPGMGEQSASVLQQNLFR*L*EVALHSYILRI
LSRGEDSS*NSKFLWR*ETFL**RLQIIIQTGFC
QTFRIEMCYLQLLFSAM*EGSN*RTRWCCERFL
Q*RLL*KISGLVLQGCKV*LL*ISRNS*RASSV
AWGNETFL*STLLTAFLLSTK*AQHDNSERT*KL
TL*SGLSDISNQNDRFSTTPFSNT*QRDEEQSS
SLQTFNNDKSYLL*TSHADQILSDR*YLEDRIC
SSAYPCACVYPSSYAHVQSEYSCSYYSSCSCASS
CFSACSIGQQ*EDSCSN*GAKKQGFFRCS*YRV
AYNDGYDE*RRGENRDNQHQQCNY*NRYNWFRP
FEEL*PRDTVQHA*CTI*TRFGYRNRFSQSC*GA
*YGK*IFITTCFWRRI*GTAQTSI*KKGSQEKG
CIRIPVS***F*QFRMQLSFQIYVWRKCMETLG
QN*AT**RSSGIR*VKIF*ISKVKRGSTLSHHS*
A*LWVSSFCQ*DPTAKWRELCT*QHLLPLPWNT
GVFVWK*SKRQHIY*SWIPNI*ARIE*NTAKLA
TKHTSRWVNILSS*RRLSLEDKTTRITLSSSSSE
YTVLL*H*VFWPENSGTTLKTFLWHCV*ALEKK
SFNDGKQSVSSIPSVFLVWNR**R*NYYWKKKT
*R**ASI*TN*KHSQSFQMSCENV*MLLV*KSTE
S*SEDGCFLFATRML*FYR*PCLVYVYFTGPKH
LGKYACTGSSSKRYL**RQL*TG*RHRLKRNVA
EAIGIKQH*IVMLLDLYYGKHFKFTPSVLSFVAV
YPRWVLPCK*SVSESCHYSM*WF*DT*QIHSNS
FFIIIYLIRYVCNFLHYRI*MRMCHV*FFSCSK
KHPYCTTLLLQSFLGRGLFYWVLNQMVVYG*HY*
KFRTCSVFRNF*NKL*TNRLGFLFCFGVLFCLV
LHFSY*SLTRLCREIPSSVPKIDKRMLSILVYC
NVNLCW*KQFFVPLIKTLVSFSHLWLSASPNQ*K
TNIYMYIYVCMCVCIYIYIYIYI

Frame 2

RAEGGASPGSAAPPPPPPPPPHQR*SRDWTERS
CACRRRGVGRGRGGSFRGAPARTGTKNRLQPCL
VHPWQKVRRKTPPLFFGMDTSSVGGLELTDQTP
VLLGSTAMATSLTNVGNSFSGPANPLVSRSNKFQ
NSSVEDDDDVVFIEPVQPPPPSVPVVADQRTIT
FTSSKNEELQGNDSKITPSSKELASQKGSVSET
IVIDDEEDMETNQGQEKNSSNFIERRPPETKNRT
NDVDFSTSSFSRSKVNAGMGNSGITTEPDSEIQ
IANVTTLETGVSSVNDGQLENTDGRDMNLMITH
VTSLQNTNLGDVSNGLQSSNFGVNIQTYTPSLTS
QTKTGVGPFNPGRMNVAGDVFQNGESATHHNPD
SWISQSASFPRNQKQPGVDSLSPVASLPKQIFQ
PSVQQQPTKPVKVTCANCKKPLQKGQTAYQRKGS
AHLFCSTTCLSSFSHKPAPKKLCVMCKKDITTM
KGTIVAQVDSSESFQEFCSTSCLSLYEDKQNPT
KGALNKSRCTICGKLTEIRHEVSFKNMTHKLCSD
HCFNRYRMANGLIMNCCEQCGEYLPSKGAGNNV
LVIDGQQKRFCCQSCVSEYKQVGSHPSFLKEVR
DHMQDSFLMQPEKYGKLTTCTGCRTQCRFFDMTQ
CIGPNGYMEPYCSTACMNSHKTKYAKSQSLGII
CHFCKRNSLPQYQATMPDGKLYNFCNSSCVAKF
QALSMQSSPNGQFVAPSDIQLKCNYCKNSFCSKP
EILEWENKVHQFCSKTCSDDYKKLHCIVTYCEY
CQEEKTLHETVNFSGVKRPFCSEGCKLLYKQDF
ARRLGLRCVTCNYCSQLCKKGATKELDGVVRDFC
SEDCCKKFQDWYYKAARCDCCKSQGTLKERVQW
RGEMKHFCDQHCLLRFYCQQNEPNMTTQKGPEN
LHYDQGCQTSRTKMTGSAPPPSPTPNKEMKNKAV
LCKPLTMTKATYCKPHMQTKSCQTDDTWRTEYV
PVPIPVPVYIPVPMHMYSQNIPVPTTVPVPVPV
PVFLPAPLDSSEKIPAAIEELKSKVSSDALDTEL
LTMTDMMSEDEGKTETTNINSVIIETDIIGSDL
LKNSDPETQSSMPDVPYEPDLDIEIDFPRAAEE
LDMENEFLLPPVFGEEYEEQPRPRSKKKGAKRKA
VSGYQSHDDSSDNSECSFPFKYTYGVNAWKHWV
KTRQLDEDLLVLDELKSSKSVKLKEDLLSHTTA
ELNYGLAHFVNEIRRPNGENYAPDSIYYLCLGIQ
EYLCGSNRKDNIFIDPGYQTFEQELNKILRSWQ
PSILPDGSIFSRVEEDYLWRIKQLGSHSPVALL
NTLFYFNTKYFGLKTVEQHLRLSFGTVFRHWKKN
PLTMENKACLRYQVSSLCGTDNEDKITTGKRKH
EDDEPVFEQIENTANPSRCPVKMFECYLSKSPQ
NLNQRMDVFYLQPECSSSTDSPVWYTSTSLDRNT
LENMLVRVLLVKDIYDKDNYELDEDTD*KGTLQ
KQSG*NSIR*SCC*IFIMENISSLLLLF*VL*Q
CTHAGYYHVNNL*VKVAIILCSGFRILNKYIQIL
FLLLFI*LGMFVTFYITEYE*ECAMYNFFLVVR
NIHIAQLYCCKASLEGGSFTGFLTRWLCMGSTT
KSLELAVSFGIFKINCKLIGWGFCFVLGFCFVWF
YILVTEALQGYVERYHLLYQK*TRECCQYWCTV
M*IYAGENNFLFPLLKP*CPFLICGFLHHPINK
KQIYICIYMYVCVCVYIYIYIYI

Sequence NM_003453

AGCGCGCGGAGGGCGGGGCCTCCCCGGGCTCGGCCGCGCCGCCGCCTCCTCCGCCTCCTCCTCCTCACCAGCGCTAAAGCCGGGACTGGACCGAGCGGAG
TTGTGCGTGTCGCCGAAGGGGGGTGGGCCGGGGGAGGGGAGGTTCGTTCCGCGGAGCCCCAGCCAGAACCGGGACCAAGAATCGCCTTCAGCCCTGTCTG
GTGCATCCTTGGCAGAAAGTGAGGAGGAAAACACCCCCATTGTTAGATGAGGTCTTAATCATGGAACATTTGAAGTCACGTAAAGAAGGTTCTTTGGCAT
GGACACAAGTTCAGTGGGAGGATTAGAATTGACTGATCAGACTCCTGTTTTATTAGGGAGTACGGCCATGGCAACTAGTCTCACGAATGTAGGAAACTCA
TTTAGTGGTCCAGCTAATCCTTTAGTGTCTAGATCTAATAAGTTTCAGAACTCGTCAGTGGAAGATGATGATGATGTTGTTTTTATCGAACCTGTACAAC
CTCCCCCACCTTCTGTACCAGTGGTAGCTGATCAAAGAACCATAACATTTACATCATCAAAAAATGAAGAACTACAAGGAAATGATTCCAAAATTACTCC
TTCCTCAAAAGAGTTGGCATCTCAGAAGGGAAGTGTAAGTGAGACAATTGTCATTGATGATGAAGAGGACATGGAAACAAATCAAGGGCAAGAGAAAAAT
TCCTCCAATTTTATTGAACGAAGACCTCCTGAGACTAAAAACAGAACCAATGATGTGGATTTCTCCACTTCCAGTTTTTCAAGAAGTAAGGTAAATGCAG
GAATGGGTAATAGTGGTATCACCACAGAACCAGACTCTGAAATTCAGATTGCTAATGTTACAACTTTAGAAACAGGTGTAAGCTCTGTGAATGATGGCCA
ATTAGAAAATACTGACGGGCGAGATATGAACTTAATGATTACACATGTAACATCACTGCAGAATACCAACTTGGGAGATGTCTCTAACGGACTGCAGTCA
AGTAATTTTGGTGTTAATATACAAACATACACCCCATCTTTAACTTCACAGACCAAGACTGGAGTAGGACCTTTTAATCCTGGTAGAATGAATGTGGCAG
GAGACGTTTTTCAGAATGGAGAATCTGCAACTCATCATAATCCTGATTCTTGGATCTCCCAGTCAGCTTCATTTCCCCGTAATCAGAAACAACCAGGGGT
GGACTCTTTATCACCAGTGGCCTCACTTCCTAAACAGATTTTCCAGCCGTCTGTCCAACAGCAGCCTACTAAACCAGTTAAAGTCACTTGTGCAAACTGC
AAAAAACCTTTACAGAAGGGCCAGACAGCTTATCAACGAAAAGGATCAGCTCACCTCTTTTGTTCTACCACCTGCCTTTCTTCCTTCTCCCACAAGCCTG
CTCCAAAGAAACTCTGTGTTATGTGTAAAAAAGATATAACTACAATGAAAGGAACCATTGTTGCTCAAGTGGATTCAAGTGAGTCCTTCCAGGAATTCTG
TAGTACATCTTGTTTATCTCTCTATGAAGACAAACAGAATCCTACTAAAGGAGCTCTAAATAAATCAAGATGTACAATCTGTGGTAAACTAACTGAGATT
CGCCATGAAGTCAGCTTTAAAAATATGACTCATAAGCTGTGCAGTGACCACTGCTTTAATAGATATAGAATGGCCAATGGTTTAATAATGAATTGCTGTG
AACAGTGTGGAGAGTACTTGCCCAGTAAAGGTGCTGGAAATAATGTTCTGGTGATTGATGGTCAACAGAAAAGATTTTGCTGTCAAAGTTGTGTCAGTGA
ATACAAACAGGTAGGTAGCCATCCAAGCTTCCTGAAGGAGGTTCGAGATCACATGCAGGACTCTTTCTTAATGCAGCCTGAGAAATATGGAAAACTGACA
ACTTGTACTGGTTGCCGAACACAGTGCAGGTTTTTTGATATGACTCAGTGTATAGGTCCTAATGGATATATGGAGCCATATTGTTCAACTGCTTGTATGA
ACAGTCACAAGACAAAATATGCAAAATCACAAAGTTTGGGAATTATTTGCCATTTTTGTAAGCGAAACTCTTTACCTCAATACCAAGCCACAATGCCTGA
TGGAAAACTGTACAACTTTTGCAATTCCAGTTGTGTGGCTAAATTTCAGGCTCTAAGTATGCAGTCATCTCCAAATGGCCAGTTTGTAGCGCCAAGTGAT
ATTCAGTTGAAATGCAACTACTGCAAAAATTCCTTTTGTTCAAAACCAGAAATCCTGGAATGGGAGAACAAAGTGCATCAGTTCTGCAGCAAAACTTGTT
CAGATGACTATAAGAAGTTGCATTGCATAGTTACATATTGCGAATACTGTCAAGAGGAGAAGACTCTTCATGAAACAGTAAATTTCTCTGGCGTTAAGAG
ACCTTTCTGTAGTGAAGGCTGCAAATTATTATACAAACAGGATTTTGCCAGACGTTTAGGATTGAGATGTGTTACTTGCAACTATTGTTCTCAGCTATGT
AAGAAGGGAGCAACTAAAGAACTCGATGGTGTTGTGAGAGATTTCTGCAGTGAAGATTGCTGTAAAAAATTTCAGGATTGGTACTACAAGGCTGCAAGGT
GTGACTGTTGTAAATCTCAAGGAACTCTTAAAGAGCGAGTTCAGTGGCGTGGGGAAATGAAACATTTCTGTGATCAACATTGCTTACTGCGTTTCTACTG
TCAACAAAATGAGCCCAACATGACAACTCAGAAAGGACCTGAAAACTTACATTATGATCAGGGTTGTCAGACATCTCGAACCAAAATGACAGGTTCAGCA
CCACCCCCTTCTCCAACACCTAACAAAGAGATGAAGAACAAAGCAGTTCTTTGCAAACCTTTAACAATGACAAAAGCTACTTACTGTAAACCTCACATGC
AGACCAAATCTTGTCAGACAGATGATACTTGGAGGACAGAATATGTTCCAGTGCCTATCCCTGTGCCTGTGTATATCCCAGTTCCTATGCACATGTACAG
TCAGAATATTCCTGTTCCTACTACAGTTCCTGTTCCTGTGCCAGTTCCTGTTTTTCTGCCTGCTCCATTGGACAGCAGTGAGAAGATTCCTGCAGCAATT
GAGGAGCTAAAAAGCAAGGTTTCTTCAGATGCTCTTGATACAGAGTTGCTTACAATGACGGATATGATGAGTGAAGACGAGGGGAAAACAGAGACAACCA
ACATCAACAGTGTAATTATTGAAACAGATATAATTGGTTCAGACCTTTTGAAGAACTCTGACCCAGAGACACAGTCCAGCATGCCTGATGTACCATATGA
ACCAGATTTGGATATCGAAATAGATTTTCCCAGAGCTGCTGAGGAGCTTGATATGGAAAATGAATTTTTATTACCACCTGTTTTTGGCGAAGAATATGAG
GAACAGCCCAGACCTCGATCTAAAAAAAAGGGAGCCAAGAGAAAGGCTGTATCAGGATACCAGTCTCATGATGATAGTTCTGACAATTCAGAATGCAGCT
TTCCTTTCAAATATACGTATGGCGTAAATGCATGGAAACACTGGGTCAAAACTAGGCAACTTGATGAAGATCTTCTGGTATTAGATGAGTTAAAATCTTC
TAAATCAGTAAAGTTAAAAGAGGATCTACTCTCTCACACCACAGCTGAGCTTAACTATGGGTTAGCTCATTTTGTCAATGAGATCCGACGGCCAAATGGA
GAGAATTATGCACCTGACAGCATCTATTACCTTTGCCTTGGAATACAGGAGTATTTGTGTGGAAGTAATCGAAAAGACAACATATTTATTGATCCTGGAT
ACCAAACATTTGAGCAAGAATTGAATAAAATACTGCGAAGCTGGCAACCAAGCATACTTCCAGATGGGTCAATATTCTCTCGAGTTGAAGAAGACTATCT
CTGGAGGATAAAACAACTAGGATCACACTCTCCAGTAGCTCTTCTGAATACACTGTTCTACTTTAACACTAAGTATTTTGGCCTGAAAACAGTGGAACAA
CACTTAAGACTTTCCTTTGGCACTGTGTTTAGGCATTGGAAAAAAAATCCTTTAACGATGGAAAACAAAGCGTGTCTTCGATACCAAGTGTCTTCCTTGT
GTGGAACAGATAATGAAGATAAAATTACTACTGGAAAAAGAAAACATGAAGATGATGAGCCAGTATTTGAACAAATTGAAAACACAGCCAATCCTTCCAG
ATGTCCTGTGAAAATGTTTGAATGCTACTTGTCTAAAAGTCCACAGAATCTTAATCAGAGGATGGATGTTTTTTATTTGCAACCAGAATGCTCTAGTTCT
ACAGATAGCCCTGTCTGGTATACGTCTACTTCACTGGACCGAAACACCTTGGAAAATATGCTTGTACGGGTTCTTCTAGTAAAAGATATTTATGATAAAG
ACAATTATGAACTGGATGAAGACACAGACTAAAAAGGAACGTTGCAGAAGCAATCGGGATAAAACAGCATTAGATAGTCATGCTGCTAGATCTTTATTAT
GGAAAACATTTCAAGTTTACTCCTTCTGTTTTGAGTTTTGTAGCAGTGTACCCACGCTGGGTATTACCATGTAAATAATCTGTGAGTGAAAGTTGCCATT
ATTCTATGTAGTGGTTTTAGGATACTTAACAAATACATTCAAATTCTTTTTTTATTATTATTTATTTGATTAGGTATGTTTGTAACTTTTTACATTACAG
AATATGAATGAGAATGTGCCATGTATAATTTTTTTCTTGTAGTAAGAAACATCCATATTGCACAACTCTACTGTTGCAAAGCTTCCTTGGAAGGGGGCTC
TTTTACTGGGTTCTTAACCAGATGGTTGTGTATGGGTAGCACTACTAAAAGTTTAGAACTTGCAGTGTCTTTCGGAATTTTTAAAATAAACTGTAAACTA
ATAGGCTGGGGTTTTTGTTTTGTTTTGGGGTTTTGTTTTGTTTGGTTTTACATTTTAGTTACTGAAGCCTTACAAGGTTATGTAGAGAGATACCATCTTC
TGTACCAAAAATAGACAAGAGAATGCTGTCAATATTGGTGTACTGTAATGTGAATCTATGCTGGTGAAAACAATTTTTTGTTCCCCTTATTAAAACCTTA
GTGTCCTTTTCTCATTTGTGGCTTTCTGCATCACCCAATCAATAAAAAACAAATATATATATGTATATATATGTATGTATGTGTGTGTGTATATATATAT
ATATATATATATATATA
  • refseq_ZMYM2.F2 (forward primer)
  • refseq_ZMYM2.R2 (reverse primer)
  • Single exon skipping

Frame 0

SARRAGPPRARPRRRLLRLLLLTSAKAGTGPSG
VVRVAEGGWAGGGEVRSAEPQPEPGPRIAFSPVW
CILGRK*GGKHPHC*MRS*SWNI*SHVKKVLWH
GHKFSGRIRID*SDSCFIREYGHGN*SHECRKL
I*WSS*SFSV*I**VSELVSGR***CCFYRTCTT
SPTFCTSGS*SKNHNIYIIKK*RTTRK*FQNYS
FLKRVGISEGKCK*DNCH***RGHGNKSRAREK
FLQFY*TKTS*D*KQNQ*CGFLHFQFFKK*GKCR
NG**WYHHRTRL*NSDC*CYNFRNRCKLCE*WP
IRKY*RARYELNDYTCNITAEYQLGRCL*RTAV
K*FWC*YTNIHPIFNFTDQDWSRTF*SW*NECGR
RRFSEWRICNSS*S*FLDLPVSFISP*SETTRG
GLFITSGLTS*TDFPAVCPTAAY*TS*SHLCKL
QKTFTEGPDSLSTKRISSPLLFYHLPFFLLPQAC
SKETLCYV*KRYNYNERNHCCSSGFK*VLPGIL
*YILFISL*RQTESY*RSSK*IKMYNLW*TN*D
SP*SQL*KYDS*AVQ*PLL**I*NGQWFNNELL*
TVWRVLAQ*RCWK*CSGD*WSTEKILLSKLCQ*
IQTGR*PSKLPEGGSRSHAGLFLNAA*EIWKTD
NLYWLPNTVQVF*YDSVYRS*WIYGAILFNCLYE
QSQDKICKITKFGNYLPFL*AKLFTSIPSHNA*
WKTVQLLQFQLCG*ISGSKYAVISKWPVCSAK*
YSVEMQLLQKFLLFKTRNPGMGEQSASVLQQNLF
R*L*EVALHSYILRILSRGEDSS*NSKFLWR*E
TFL**RLQIIIQTGFCQTFRIEMCYLQLLFSAM
*EGSN*RTRWCCERFLQ*RLL*KISGLVLQGCKV
*LL*ISRNS*RASSVAWGNETFL*STLLTAFLL
STK*AQHDNSERT*KLTL*SGLSDISNQNDRFS
TTPFSNT*QRDEEQSSSLQTFNNDKSYLL*TSHA
DQILSDR*YLEDRICSSAYPCACVYPSSYAHVQ
SEYSCSYYSSCSCASSCFSACSIGQQ*EDSCSN
*GAKKQGFFRCS*YRVAYNDGYDE*RRGENRDNQ
HQQCNY*NRYNWFRPFEEL*PRDTVQHA*CTI*
TRFGYRNRFSQSC*GA*YGK*IFITTCFWRRI*
GTAQTSI*KKGSQEKGCIRIPVS***F*QFRMQL
SFQIYVWRKCMETLGQN*AT**RSSGIR*VKIF
*ISKVKRGSTLSHHS*A*LWVSSFCQ*DPTAKW
RELCT*QHLLPLPWNTGVFVWK*SKRQHIY*SWI
PNI*ARIE*NTAKLATKHTSRWVNILSS*RRLS
LEDKTTRITLSSSSSEYTVLL*H*VFWPENSGT
TLKTFLWHCV*ALEKKSFNDGKQSVSSIPSVFLV
WNR**R*NYYWKKKT*R**ASI*TN*KHSQSFQ
MSCENV*MLLV*KSTES*SEDGCFLFATRML*F
YR*PCLVYVYFTGPKHLGKYACTGSSSKRYL**R
QL*TG*RHRLKRNVAEAIGIKQH*IVMLLDLYY
GKHFKFTPSVLSFVAVYPRWVLPCK*SVSESCH
YSM*WF*DT*QIHSNSFFIIIYLIRYVCNFLHYR
I*MRMCHV*FFSCSKKHPYCTTLLLQSFLGRGL
FYWVLNQMVVYG*HY*KFRTCSVFRNF*NKL*T
NRLGFLFCFGVLFCLVLHFSY*SLTRLCREIPSS
VPKIDKRMLSILVYCNVNLCW*KQFFVPLIKTL
VSFSHLWLSASPNQ*KTNIYMYIYVCMCVCIYI
YIYIYI

Frame 1

ARGGRGLPGLGRAAASSASSSSPALKPGLDRAE
LCVSPKGGGPGEGRFVPRSPSQNRDQESPSALS
GASLAESEEENTPIVR*GLNHGTFEVT*RRFFGM
DTSSVGGLELTDQTPVLLGSTAMATSLTNVGNS
FSGPANPLVSRSNKFQNSSVEDDDDVVFIEPVQ
PPPPSVPVVADQRTITFTSSKNEELQGNDSKITP
SSKELASQKGSVSETIVIDDEEDMETNQGQEKN
SSNFIERRPPETKNRTNDVDFSTSSFSRSKVNA
GMGNSGITTEPDSEIQIANVTTLETGVSSVNDGQ
LENTDGRDMNLMITHVTSLQNTNLGDVSNGLQS
SNFGVNIQTYTPSLTSQTKTGVGPFNPGRMNVA
GDVFQNGESATHHNPDSWISQSASFPRNQKQPGV
DSLSPVASLPKQIFQPSVQQQPTKPVKVTCANC
KKPLQKGQTAYQRKGSAHLFCSTTCLSSFSHKP
APKKLCVMCKKDITTMKGTIVAQVDSSESFQEFC
STSCLSLYEDKQNPTKGALNKSRCTICGKLTEI
RHEVSFKNMTHKLCSDHCFNRYRMANGLIMNCC
EQCGEYLPSKGAGNNVLVIDGQQKRFCCQSCVSE
YKQVGSHPSFLKEVRDHMQDSFLMQPEKYGKLT
TCTGCRTQCRFFDMTQCIGPNGYMEPYCSTACM
NSHKTKYAKSQSLGIICHFCKRNSLPQYQATMPD
GKLYNFCNSSCVAKFQALSMQSSPNGQFVAPSD
IQLKCNYCKNSFCSKPEILEWENKVHQFCSKTC
SDDYKKLHCIVTYCEYCQEEKTLHETVNFSGVKR
PFCSEGCKLLYKQDFARRLGLRCVTCNYCSQLC
KKGATKELDGVVRDFCSEDCCKKFQDWYYKAAR
CDCCKSQGTLKERVQWRGEMKHFCDQHCLLRFYC
QQNEPNMTTQKGPENLHYDQGCQTSRTKMTGSA
PPPSPTPNKEMKNKAVLCKPLTMTKATYCKPHM
QTKSCQTDDTWRTEYVPVPIPVPVYIPVPMHMYS
QNIPVPTTVPVPVPVPVFLPAPLDSSEKIPAAI
EELKSKVSSDALDTELLTMTDMMSEDEGKTETT
NINSVIIETDIIGSDLLKNSDPETQSSMPDVPYE
PDLDIEIDFPRAAEELDMENEFLLPPVFGEEYE
EQPRPRSKKKGAKRKAVSGYQSHDDSSDNSECS
FPFKYTYGVNAWKHWVKTRQLDEDLLVLDELKSS
KSVKLKEDLLSHTTAELNYGLAHFVNEIRRPNG
ENYAPDSIYYLCLGIQEYLCGSNRKDNIFIDPG
YQTFEQELNKILRSWQPSILPDGSIFSRVEEDYL
WRIKQLGSHSPVALLNTLFYFNTKYFGLKTVEQ
HLRLSFGTVFRHWKKNPLTMENKACLRYQVSSL
CGTDNEDKITTGKRKHEDDEPVFEQIENTANPSR
CPVKMFECYLSKSPQNLNQRMDVFYLQPECSSS
TDSPVWYTSTSLDRNTLENMLVRVLLVKDIYDK
DNYELDEDTD*KGTLQKQSG*NSIR*SCC*IFIM
ENISSLLLLF*VL*QCTHAGYYHVNNL*VKVAI
ILCSGFRILNKYIQILFLLLFI*LGMFVTFYIT
EYE*ECAMYNFFLVVRNIHIAQLYCCKASLEGGS
FTGFLTRWLCMGSTTKSLELAVSFGIFKINCKL
IGWGFCFVLGFCFVWFYILVTEALQGYVERYHL
LYQK*TRECCQYWCTVM*IYAGENNFLFPLLKP*
CPFLICGFLHHPINKKQIYICIYMYVCVCVYIY
IYIYI

Frame 2

RAEGGASPGSAAPPPPPPPPPHQR*SRDWTERS
CACRRRGVGRGRGGSFRGAPARTGTKNRLQPCL
VHPWQKVRRKTPPLLDEVLIMEHLKSRKEGSLA
WTQVQWED*N*LIRLLFY*GVRPWQLVSRM*ETH
LVVQLIL*CLDLISFRTRQWKMMMMLFLSNLYN
LPHLLYQW*LIKEP*HLHHQKMKNYKEMIPKLL
LPQKSWHLRREV*VRQLSLMMKRTWKQIKGKRKI
PPILLNEDLLRLKTEPMMWISPLPVFQEVR*MQ
EWVIVVSPQNQTLKFRLLMLQL*KQV*AL*MMA
N*KILTGEI*T**LHM*HHCRIPTWEMSLTDCSQ
VILVLIYKHTPHL*LHRPRLE*DLLILVE*MWQ
ETFFRMENLQLIIILILGSPSQLHFPVIRNNQG
WTLYHQWPHFLNRFSSRLSNSSLLNQLKSLVQTA
KNLYRRARQLINEKDQLTSFVLPPAFLPSPTSL
LQRNSVLCVKKI*LQ*KEPLLLKWIQVSPSRNS
VVHLVYLSMKTNRILLKEL*INQDVQSVVN*LRF
AMKSALKI*LISCAVTTALIDIEWPMV***IAV
NSVESTCPVKVLEIMFW*LMVNRKDFAVKVVSV
NTNR*VAIQAS*RRFEITCRTLS*CSLRNMEN*Q
LVLVAEHSAGFLI*LSV*VLMDIWSHIVQLLV*
TVTRQNMQNHKVWELFAIFVSETLYLNTKPQCL
MENCTTFAIPVVWLNFRL*VCSHLQMASL*RQVI
FS*NATTAKIPFVQNQKSWNGRTKCISSAAKLV
QMTIRSCIA*LHIANTVKRRRLFMKQ*ISLALR
DLSVVKAANYYTNRILPDV*D*DVLLATIVLSYV
RREQLKNSMVL*EISAVKIAVKNFRIGTTRLQG
VTVVNLKELLKSEFSGVGK*NISVINIAYCVST
VNKMSPT*QLRKDLKTYIMIRVVRHLEPK*QVQH
HPLLQHLTKR*RTKQFFANL*Q*QKLLTVNLTC
RPNLVRQMILGGQNMFQCLSLCLCISQFLCTCT
VRIFLFLLQFLFLCQFLFFCLLHWTAVRRFLQQL
RS*KARFLQMLLIQSCLQ*RI**VKTRGKQRQP
TSTV*LLKQI*LVQTF*RTLTQRHSPACLMYHM
NQIWISK*IFPELLRSLIWKMNFYYHLFLAKNMR
NSPDLDLKKREPRERLYQDTSLMMIVLTIQNAA
FLSNIRMA*MHGNTGSKLGNLMKIFWY*MS*NL
LNQ*S*KRIYSLTPQLSLTMG*LILSMRSDGQME
RIMHLTASITFALEYRSICVEVIEKTTYLLILD
TKHLSKN*IKYCEAGNQAYFQMGQYSLELKKTI
SGG*NN*DHTLQ*LF*IHCSTLTLSILA*KQWNN
T*DFPLALCLGIGKKIL*RWKTKRVFDTKCLPC
VEQIMKIKLLLEKENMKMMSQYLNKLKTQPILP
DVL*KCLNATCLKVHRILIRGWMFFICNQNALVL
QIALSGIRLLHWTETPWKICLYGFF**KIFMIK
TIMNWMKTQTKKERCRSNRDKTALDSHAARSLL
WKTFQVYSFCFEFCSSVPTLGITM*IICE*KLPL
FYVVVLGYLTNTFKFFFYYYLFD*VCL*LFTLQ
NMNENVPCIIFFL**ETSILHNSTVAKLPWKGA
LLLGS*PDGCVWVALLKV*NLQCLSEFLK*TVN*
*AGVFVLFWGFVLFGFTF*LLKPYKVM*RDTIF
CTKNRQENAVNIGVL*CESMLVKTIFCSPY*NL
SVLFSFVAFCITQSIKNKYIYVYICMYVCVYIYI
YIYIY

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