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Sequences

Sequence NM_003600

ACAAGGCAGCCTCGCTCGAGCGCAGGCCAATCGGCTTTCTAGCTAGAGGGTTTAACTCCTATTTAAAAAGAAGAACCTTTGAATTCTAACGGCTGAGCTC TTGGAAGACTTGGGTCCTTGGGTCGCAGGTGGGAGCCGACGGGTGGGTAGACCGTGGGGGATATCTCAGTGGCGGACGAGGACGGCGGGGACAAGGGGCG GCTGGTCGGAGTGGCGGAGCGTCAAGTCCCCTGTCGGTTCCTCCGTCCCTGAGTGTCCTTGGCGCTGCCTTGTGCCCGCCCAGCGCCTTTGCATCCGCTC CTGGGCACCGAGGCGCCCTGTAGGATACTGCTTGTTACTTATTACAGCTAGAGGCATCATGGACCGATCTAAAGAAAACTGCATTTCAGGACCTGTTAAG GCTACAGCTCCAGTTGGAGGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTCAGAATCCATTACCTGTAAATAGTGGCCAGGCTCAGCGGGTCT TGTGTCCTTCAAATTCTTCCCAGCGCATTCCTTTGCAAGCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAATCAGAAGCAGAAGCAATTGCAGGC AACCAGTGTACCTCATCCTGTCTCCAGGCCACTGAATAACACCCAAAAGAGCAAGCAGCCCCTGCCATCGGCACCTGAAAATAATCCTGAGGAGGAACTG GCATCAAAACAGAAAAATGAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGAAATTGGTCGCCCTCTGGGTAAAGGAAAGTTTGGTAATGTTT ATTTGGCAAGAGAAAAGCAAAGCAAGTTTATTCTGGCTCTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGAGTGGAGCATCAGCTCAGAAGAGA AGTAGAAATACAGTCCCACCTTCGGCATCCTAATATTCTTAGACTGTATGGTTATTTCCATGATGCTACCAGAGTCTACCTAATTCTGGAATATGCACCA CTTGGAACAGTTTATAGAGAACTTCAGAAACTTTCAAAGTTTGATGAGCAGAGAACTGCTACTTATATAACAGAATTGGCAAATGCCCTGTCTTACTGTC ATTCGAAGAGAGTTATTCATAGAGACATTAAGCCAGAGAACTTACTTCTTGGATCAGCTGGAGAGCTTAAAATTGCAGATTTTGGGTGGTCAGTACATGC TCCATCTTCCAGGAGGACCACTCTCTGTGGCACCCTGGACTACCTGCCCCCTGAAATGATTGAAGGTCGGATGCATGATGAGAAGGTGGATCTCTGGAGC CTTGGAGTTCTTTGCTATGAATTTTTAGTTGGGAAGCCTCCTTTTGAGGCAAACACATACCAAGAGACCTACAAAAGAATATCACGGGTTGAATTCACAT TCCCTGACTTTGTAACAGAGGGAGCCAGGGACCTCATTTCAAGACTGTTGAAGCATAATCCCAGCCAGAGGCCAATGCTCAGAGAAGTACTTGAACACCC CTGGATCACAGCAAATTCATCAAAACCATCAAATTGCCAAAACAAAGAATCAGCTAGCAAACAGTCTTAGGAATCGTGCAGGGGGAGAAATCCTTGAGCC AGGGCTGCCATATAACCTGACAGGAACATGCTACTGAAGTTTATTTTACCATTGACTGCTGCCCTCAATCTAGAACGCTACACAAGAAATATTTGTTTTA CTCAGCAGGTGTGCCTTAACCTCCCTATTCAGAAAGCTCCACATCAATAAACATGACACTCTGAAGTGAAAGTAGCCACGAGAATTGTGCTACTTATACT GGTTCATAATCTGGAGGCAAGGTTCGACTGCAGCCGCCCCGTCAGCCTGTGCTAGGCATGGTGTCTTCACAGGAGGCAAATCCAGAGCCTGGCTGTGGGG AAAGTGACCACTCTGCCCTGACCCCGATCAGTTAAGGAGCTGTGCAATAACCTTCCTAGTACCTGAGTGAGTGTGTAACTTATTGGGTTGGCGAAGCCTG GTAAAGCTGTTGGAATGAGTATGTGATTCTTTTTAAGTATGAAAATAAAGATATATGTACAGACTTGTATTTTTTCTCTGGTGGCATTCCTTTAGGAATG CTGTGTGTCTGTCCGGCACCCCGGTAGGCCTGATTGGGTTTCTAGTCCTCCTTAACCACTTATCTCCCATATGAGAGTGTGAAAAATAGGAACACGTGCT CTACCTCCATTTAGGGATTTGCTTGGGATACAGAAGAGGCCATGTGTCTCAGAGCTGTTAAGGGCTTATTTTTTTAAAACATTGGAGTCATAGCATGTGT GTAAACTTTAAATATGCAAATAAATAAGTATCTATGTCT

refseq_AURKA.F2 (forward primer)
refseq_AURKA.R2 (reverse primer)
Multiple exon skipping

Frame 0

TRQPRSSAGQSAF*LEGLTPI*KEEPLNSNG*A LGRLGSLGRRWEPTGG*TVGDISVADEDGGDKGR LVGVAERQVPCRFLRP*VSLALPCARPAPLHPL LGTEAPCRILLVTYYS*RHHGPI*RKLHFRTC* GYSSSWRSKTCSRDSAISLSESITCK*WPGSAGL VSFKFFPAHSFASTKACLQSQAGSESEAEAIAG NQCTSSCLQATE*HPKEQAAPAIGT*K*S*GGT GIKTEK*RIKKEAVGFGRL*NWSPSG*RKVW*CL FGKRKAKQVYSGS*SVI*SSAGESRSGASAQKR SRNTVPPSAS*YS*TVWLFP*CYQSLPNSGICT TWNSL*RTSETFKV**AENCYLYNRIGKCPVLLS FEESYS*RH*ARELTSWISWRA*NCRFWVVSTC SIFQEDHSLWHPGLPAP*ND*RSDA**EGGSLE PWSSLL*IFSWEASF*GKHIPRDLQKNITG*IHI P*LCNRGSQGPHFKTVEA*SQPEANAQRST*TP LDHSKFIKTIKLPKQRIS*QTVLGIVQGEKSLS QGCHIT*QEHATEVYFTIDCCPQSRTLHKKYLFY SAGVP*PPYSESSTSINMTL*SESSHENCATYT GS*SGGKVRLQPPRQPVLGMVSSQEANPEPGCG ESDHSALTPIS*GAVQ*PS*YLSECVTYWVGEAW *SCWNEYVILFKYENKDICTDLYFFSGGIPLGM LCVCPAPR*A*LGF*SSLTTYLPYESVKNRNTC STSI*GFAWDTEEAMCLRAVKGLFF*NIGVIACV *TLNMQINKYLC

Frame 1

QGSLARAQANRLSS*RV*LLFKKKNL*ILTAEL LEDLGPWVAGGSRRVGRPWGISQWRTRTAGTRG GWSEWRSVKSPVGSSVPECPWRCLVPAQRLCIRS WAPRRPVGYCLLLITARGIMDRSKENCISGPVK ATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRV LCPSNSSQRIPLQAQKLVSSHKPVQNQKQKQLQA TSVPHPVSRPLNNTQKSKQPLPSAPENNPEEEL ASKQKNEESKKRQWALEDFEIGRPLGKGKFGNV YLAREKQSKFILALKVLFKAQLEKAGVEHQLRRE VEIQSHLRHPNILRLYGYFHDATRVYLILEYAP LGTVYRELQKLSKFDEQRTATYITELANALSYC HSKRVIHRDIKPENLLLGSAGELKIADFGWSVHA PSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWS LGVLCYEFLVGKPPFEANTYQETYKRISRVEFT FPDFVTEGARDLISRLLKHNPSQRPMLREVLEHP WITANSSKPSNCQNKESASKQS*ESCRGRNP*A RAAI*PDRNMLLKFILPLTAALNLERYTRNICF TQQVCLNLPIQKAPHQ*T*HSEVKVATRIVLLIL VHNLEARFDCSRPVSLC*AWCLHRRQIQSLAVG KVTTLP*PRSVKELCNNLPST*VSV*LIGLAKP GKAVGMSM*FFLSMKIKIYVQTCIFSLVAFL*EC CVSVRHPGRPDWVSSPP*PLISHMRV*KIGTRA LPPFRDLLGIQKRPCVSELLRAYFFKTLES*HV CKL*ICK*ISIYV

Frame 2

KAASLERRPIGFLARGFNSYLKRRTFEF*RLSS WKTWVLGSQVGADGWVDRGGYLSGGRGRRGQGA AGRSGGASSPLSVPPSLSVLGAALCPPSAFASA PGHRGAL*DTACYLLQLEASWTDLKKTAFQDLLR LQLQLEVQNVFS*LSNFLVRIHYL*IVARLSGS CVLQILPSAFLCKHKSLSPVTSRFRIRSRSNCR QPVYLILSPGH*ITPKRASSPCHRHLKIILRRNW HQNRKMKNQKRGSGLWKTLKLVALWVKESLVMF IWQEKSKASLFWLLKCYLKLSWRKPEWSISSEE K*KYSPTFGILIFLDCMVISMMLPEST*FWNMHH LEQFIENFRNFQSLMSRELLLI*QNWQMPCLTV IRRELFIETLSQRTYFLDQLESLKLQILGGQYM LHLPGGPLSVAPWTTCPLK*LKVGCMMRRWISGA LEFFAMNF*LGSLLLRQTHTKRPTKEYHGLNSH SLTL*QREPGTSFQDC*SIIPARGQCSEKYLNT PGSQQIHQNHQIAKTKNQLANSLRNRAGGEILEP GLPYNLTGTCY*SLFYH*LLPSI*NATQEIFVL LSRCALTSLFRKLHINKHDTLK*K*PRELCYLY WFIIWRQGSTAAAPSACARHGVFTGGKSRAWLWG K*PLCPDPDQLRSCAITFLVPE*VCNLLGWRSL VKLLE*VCDSF*V*K*RYMYRLVFFLWWHSFRN AVCLSGTPVGLIGFLVLLNHLSPI*ECEK*EHVL YLHLGICLGYRRGHVSQSC*GLIFLKHWSHSMC VNFKYANK*VSMS

Sequence NM_198433

ACAAGGCAGCCTCGCTCGAGCGCAGGCCAATCGGCTTTCTAGCTAGAGGGTTTAACTCCTATTTAAAAAGAAGAACCTTTGAATTCTAACGGCTGAGCTC TTGGAAGACTTGGGTCCTTGGGTCGCAGGTGGGAGCCGACGGGTGGGTAGACCGTGGGGGATATCTCAGTGGCGGACGAGGACGGCGGGGACAAGGGGCG GCTGGTCGGAGTGGCGGAGCGTCAAGTCCCCTGTCGGTTCCTCCGTCCCTGAGTGTCCTTGGCGCTGCCTTGTGCCCGCCCAGCGCCTTTGCATCCGCTC CTGGGCACCGAGGCGCCCTGTAGGATACTGCTTGTTACTTATTACAGCTAGAGGGTCTCACTCCATTGCCCAGGCCAGAGTGCGGGGATATTTGATAAGA AACTTCAGTGAAGGCCGGGCGCGGTGGCTCATGCCCGTAATCCCAGCATTTTCGGAGGCCGAGGCTGGAGTGCAATGGTGTGATCTCAGCTCACTGCAAC CTCTGCTTCCTGGGTTTAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATCATGGACCGATCTAAAGAAAACTGCATTTCAGGAC CTGTTAAGGCTACAGCTCCAGTTGGAGGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTCAGAATCCATTACCTGTAAATAGTGGCCAGGCTCA GCGGGTCTTGTGTCCTTCAAATTCTTCCCAGCGCATTCCTTTGCAAGCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAATCAGAAGCAGAAGCAA TTGCAGGCAACCAGTGTACCTCATCCTGTCTCCAGGCCACTGAATAACACCCAAAAGAGCAAGCAGCCCCTGCCATCGGCACCTGAAAATAATCCTGAGG AGGAACTGGCATCAAAACAGAAAAATGAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGAAATTGGTCGCCCTCTGGGTAAAGGAAAGTTTGG TAATGTTTATTTGGCAAGAGAAAAGCAAAGCAAGTTTATTCTGGCTCTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGAGTGGAGCATCAGCTC AGAAGAGAAGTAGAAATACAGTCCCACCTTCGGCATCCTAATATTCTTAGACTGTATGGTTATTTCCATGATGCTACCAGAGTCTACCTAATTCTGGAAT ATGCACCACTTGGAACAGTTTATAGAGAACTTCAGAAACTTTCAAAGTTTGATGAGCAGAGAACTGCTACTTATATAACAGAATTGGCAAATGCCCTGTC TTACTGTCATTCGAAGAGAGTTATTCATAGAGACATTAAGCCAGAGAACTTACTTCTTGGATCAGCTGGAGAGCTTAAAATTGCAGATTTTGGGTGGTCA GTACATGCTCCATCTTCCAGGAGGACCACTCTCTGTGGCACCCTGGACTACCTGCCCCCTGAAATGATTGAAGGTCGGATGCATGATGAGAAGGTGGATC TCTGGAGCCTTGGAGTTCTTTGCTATGAATTTTTAGTTGGGAAGCCTCCTTTTGAGGCAAACACATACCAAGAGACCTACAAAAGAATATCACGGGTTGA ATTCACATTCCCTGACTTTGTAACAGAGGGAGCCAGGGACCTCATTTCAAGACTGTTGAAGCATAATCCCAGCCAGAGGCCAATGCTCAGAGAAGTACTT GAACACCCCTGGATCACAGCAAATTCATCAAAACCATCAAATTGCCAAAACAAAGAATCAGCTAGCAAACAGTCTTAGGAATCGTGCAGGGGGAGAAATC CTTGAGCCAGGGCTGCCATATAACCTGACAGGAACATGCTACTGAAGTTTATTTTACCATTGACTGCTGCCCTCAATCTAGAACGCTACACAAGAAATAT TTGTTTTACTCAGCAGGTGTGCCTTAACCTCCCTATTCAGAAAGCTCCACATCAATAAACATGACACTCTGAAGTGAAAGTAGCCACGAGAATTGTGCTA CTTATACTGGTTCATAATCTGGAGGCAAGGTTCGACTGCAGCCGCCCCGTCAGCCTGTGCTAGGCATGGTGTCTTCACAGGAGGCAAATCCAGAGCCTGG CTGTGGGGAAAGTGACCACTCTGCCCTGACCCCGATCAGTTAAGGAGCTGTGCAATAACCTTCCTAGTACCTGAGTGAGTGTGTAACTTATTGGGTTGGC GAAGCCTGGTAAAGCTGTTGGAATGAGTATGTGATTCTTTTTAAGTATGAAAATAAAGATATATGTACAGACTTGTATTTTTTCTCTGGTGGCATTCCTT TAGGAATGCTGTGTGTCTGTCCGGCACCCCGGTAGGCCTGATTGGGTTTCTAGTCCTCCTTAACCACTTATCTCCCATATGAGAGTGTGAAAAATAGGAA CACGTGCTCTACCTCCATTTAGGGATTTGCTTGGGATACAGAAGAGGCCATGTGTCTCAGAGCTGTTAAGGGCTTATTTTTTTAAAACATTGGAGTCATA GCATGTGTGTAAACTTTAAATATGCAAATAAATAAGTATCTATGTCT

refseq_AURKA.F2 (forward primer)
refseq_AURKA.R2 (reverse primer)
Multiple exon skipping

Frame 0

TRQPRSSAGQSAF*LEGLTPI*KEEPLNSNG*A LGRLGSLGRRWEPTGG*TVGDISVADEDGGDKGR LVGVAERQVPCRFLRP*VSLALPCARPAPLHPL LGTEAPCRILLVTYYS*RVSLHCPGQSAGIFDK KLQ*RPGAVAHARNPSIFGGRGWSAMV*SQLTAT SASWV*VILLPQPPE*LGLQASWTDLKKTAFQD LLRLQLQLEVQNVFS*LSNFLVRIHYL*IVARL SGSCVLQILPSAFLCKHKSLSPVTSRFRIRSRSN CRQPVYLILSPGH*ITPKRASSPCHRHLKIILR RNWHQNRKMKNQKRGSGLWKTLKLVALWVKESL VMFIWQEKSKASLFWLLKCYLKLSWRKPEWSISS EEK*KYSPTFGILIFLDCMVISMMLPEST*FWN MHHLEQFIENFRNFQSLMSRELLLI*QNWQMPC LTVIRRELFIETLSQRTYFLDQLESLKLQILGGQ YMLHLPGGPLSVAPWTTCPLK*LKVGCMMRRWI SGALEFFAMNF*LGSLLLRQTHTKRPTKEYHGL NSHSLTL*QREPGTSFQDC*SIIPARGQCSEKYL NTPGSQQIHQNHQIAKTKNQLANSLRNRAGGEI LEPGLPYNLTGTCY*SLFYH*LLPSI*NATQEI FVLLSRCALTSLFRKLHINKHDTLK*K*PRELCY LYWFIIWRQGSTAAAPSACARHGVFTGGKSRAW LWGK*PLCPDPDQLRSCAITFLVPE*VCNLLGW RSLVKLLE*VCDSF*V*K*RYMYRLVFFLWWHSF RNAVCLSGTPVGLIGFLVLLNHLSPI*ECEK*E HVLYLHLGICLGYRRGHVSQSC*GLIFLKHWSH SMCVNFKYANK*VSMS

Frame 1

QGSLARAQANRLSS*RV*LLFKKKNL*ILTAEL LEDLGPWVAGGSRRVGRPWGISQWRTRTAGTRG GWSEWRSVKSPVGSSVPECPWRCLVPAQRLCIRS WAPRRPVGYCLLLITARGSHSIAQARVRGYLIR NFSEGRARWLMPVIPAFSEAEAGVQWCDLSSLQ PLLPGFK*FSCLSLPSSWDYRHHGPI*RKLHFRT C*GYSSSWRSKTCSRDSAISLSESITCK*WPGS AGLVSFKFFPAHSFASTKACLQSQAGSESEAEA IAGNQCTSSCLQATE*HPKEQAAPAIGT*K*S*G GTGIKTEK*RIKKEAVGFGRL*NWSPSG*RKVW *CLFGKRKAKQVYSGS*SVI*SSAGESRSGASA QKRSRNTVPPSAS*YS*TVWLFP*CYQSLPNSGI CTTWNSL*RTSETFKV**AENCYLYNRIGKCPV LLSFEESYS*RH*ARELTSWISWRA*NCRFWVV STCSIFQEDHSLWHPGLPAP*ND*RSDA**EGGS LEPWSSLL*IFSWEASF*GKHIPRDLQKNITG* IHIP*LCNRGSQGPHFKTVEA*SQPEANAQRST *TPLDHSKFIKTIKLPKQRIS*QTVLGIVQGEKS LSQGCHIT*QEHATEVYFTIDCCPQSRTLHKKY LFYSAGVP*PPYSESSTSINMTL*SESSHENCA TYTGS*SGGKVRLQPPRQPVLGMVSSQEANPEPG CGESDHSALTPIS*GAVQ*PS*YLSECVTYWVG EAW*SCWNEYVILFKYENKDICTDLYFFSGGIP LGMLCVCPAPR*A*LGF*SSLTTYLPYESVKNRN TCSTSI*GFAWDTEEAMCLRAVKGLFF*NIGVI ACV*TLNMQINKYLC

Frame 2

KAASLERRPIGFLARGFNSYLKRRTFEF*RLSS WKTWVLGSQVGADGWVDRGGYLSGGRGRRGQGA AGRSGGASSPLSVPPSLSVLGAALCPPSAFASA PGHRGAL*DTACYLLQLEGLTPLPRPECGDI**E TSVKAGRGGSCP*SQHFRRPRLECNGVISAHCN LCFLGLSDSPASASRVAGITGIMDRSKENCISG PVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQ RVLCPSNSSQRIPLQAQKLVSSHKPVQNQKQKQ LQATSVPHPVSRPLNNTQKSKQPLPSAPENNPE EELASKQKNEESKKRQWALEDFEIGRPLGKGKFG NVYLAREKQSKFILALKVLFKAQLEKAGVEHQL RREVEIQSHLRHPNILRLYGYFHDATRVYLILE YAPLGTVYRELQKLSKFDEQRTATYITELANALS YCHSKRVIHRDIKPENLLLGSAGELKIADFGWS VHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVD LWSLGVLCYEFLVGKPPFEANTYQETYKRISRVE FTFPDFVTEGARDLISRLLKHNPSQRPMLREVL EHPWITANSSKPSNCQNKESASKQS*ESCRGRN P*ARAAI*PDRNMLLKFILPLTAALNLERYTRNI CFTQQVCLNLPIQKAPHQ*T*HSEVKVATRIVL LILVHNLEARFDCSRPVSLC*AWCLHRRQIQSL AVGKVTTLP*PRSVKELCNNLPST*VSV*LIGLA KPGKAVGMSM*FFLSMKIKIYVQTCIFSLVAFL *ECCVSVRHPGRPDWVSSPP*PLISHMRV*KIG TRALPPFRDLLGIQKRPCVSELLRAYFFKTLES* HVCKL*ICK*ISIYV