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Sequences

Sequence NM_017692

ATTTTCACAACAGCCTCCTGAGTCCCATCATTATCATGGTTTATAGGTGAGGAAATTGAGACTAGGGTGGTGAAGTCACTTGCCTCAAGATGAACAGCTG
GTAGATCACATGCGTGGTGGCACCGGGTGTGGCCCTGGAGGACTCGTGCTAGTGGATCTGTTTTTCTAGTGATTCTTTTGTTTTGTCCCTTAGAGTGATG
ATGCGGGTGTGCTGGTTGGTGAGACAGGACAGCCGGCACCAGCGAATCAGACTTCCACATTTGGAAGCAGTTGTGATTGGGCGTGGCCCAGAGACCAAGA
TCACTGATAAGAAATGTTCTCGACAGCAAGTACAGTTGAAAGCAGAGTGTAACAAGGGATATGTCAAGGTAAAGCAGAGTTTGAGGAAGAGGCAAAGAAC
CCTGGCCTGGAAACACACAGGAAGAGAAAGAGATCAGGCAACAGTGATTCTATAGAAAGGGATGCTGCTCAGGAAGCTGAGGCTGGGACAGGGCTGGAAC
CTGGGAGCAACTCTGGCCAATGCTCTGTGCCCCTAAAGAAGGGAAAAGATGCACCTATCAAAAAGGAATCCCTGGGCCACTGGAGTCAAGGCTTGAAGAT
TTCTATGCAGGACCCCAAAATGCAGGTTTACAAAGATGAGCAGGTGGTGGTGATAAAGGATAAATACCCAAAGGCCCGTTACCATTGGCTGGTCTTACCG
TGGACCTCCATTTCCAGTCTGAAGGCTGTGGCCAGGGAACACCTTGAACTCCTTAAGCATATGCACACTGTGGGGGAAAAGGTGATTGTAGATTTTGCTG
GGTCCAGCAAACTCCGCTTCCGATTGGGCTACCACGCCATTCCGAGTATGAGCCATGTACATCTTCATGTGATCAGCCAGGATTTTGATTCTCCTTGCCT
TAAAAACAAAAAACATTGGAATTCTTTCAATACAGAATACTTCCTAGAATCACAAGCTGTGATCGAGATGGTACAAGAGGCTGGTAGAGTAACTGTCCGA
GATGGGATGCCTGAGCTCTTGAAGCTGCCCCTTCGTTGTCATGAGTGCCAGCAGCTGCTGCCTTCCATTCCTCAGCTGAAAGAACATCTCAGGAAGCACT
GGACACAGTGATTCTGCAGAGCCTGAGCTGCTGCTGTGGTGTGGCCCACTGGAGCAAACTGCTGGCACCTATTCTGGGTTGCTTGTGAACTTCTACTCAT
TTCCTAAATTAAAACATGCAGCTTTTTCACAAATTTATTCTATTATTGAGTGGCCACAATGTAGAGTGGCTCAAAGTACTTCAGGATTAGGAATTTGGGT
TTGTCATAGATGTATTCTCTGGTGAGGGTGGCTGGGATATACCTGACCCACCATCTTCAGAAGGACCCATGTCAGGTCTGACCATTGGGAGCAAAGCCAT
GTTCACACTGACCTAATGCAGAGTATGGAAGCATTGGGCTGGTTATACATTTCTGTTTCTTAGATTTATCCTCCGCCTCTGTAGGCATGGACAACCTTTA
ATCAGAGCATCTAGAGTGGCCTCTTGTTTATCCTGAAGATACTGATGGGTCTTGTTTTCTGTTAGTCTGTTTTGTAATATTCTTTTCCCTTCCTTCATGG
GGAGGCTTAGTTTGTCCAGTCCTTCCATGCCCTTCTATCCCAGATTACCTAAATGTTCCCTTCTCAGGAATTCTGTCTCATCAGTTCTTCACAGTGAGAA
AAGAGGCTAGATGATGGTGTGGGGGGTTGGAGTTTTCTTCTAATACCGAGGGTTCCTGGCTGTGAGGAAACAGCCACATGTTCGTCATGATTGAGCTGTG
AAGTCTTCTTGGACCTGTTGTCTGAAAATAAAGTTAATTTGTTTGAGGCATCTCTCTTAAGTAGGTGGAAACTATTGAAGTTCAGCTAACAATCACAGCA
TAGGTTCTGATGCATGGAAAGGTGGTTGGTGAATGAAAAAGTTGCGTAGAGCCACTACTTTCTTTTTCCCTGAGAATAAATTTGGATAAAACAGTTGTA
  • refseq_APTX.F1 (forward primer)
  • refseq_APTX.R1 (reverse primer)
  • Alternative acceptor

Frame 0

IFTTAS*VPSLSWFIGEEIETRVVKSLASR*TA
GRSHAWWHRVWPWRTRASGSVFLVILLFCPLE**
CGCAGW*DRTAGTSESDFHIWKQL*LGVAQRPR
SLIRNVLDSKYS*KQSVTRDMSR*SRV*GRGKE
PWPGNTQEEKEIRQQ*FYRKGCCSGS*GWDRAGT
WEQLWPMLCAPKEGKRCTYQKGIPGPLESRLED
FYAGPQNAGLQR*AGGGDKG*IPKGPLPLAGLT
VDLHFQSEGCGQGTP*TP*AYAHCGGKGDCRFCW
VQQTPLPIGLPRHSEYEPCTSSCDQPGF*FSLP
*KQKTLEFFQYRILPRITSCDRDGTRGW*SNCP
RWDA*ALEAAPSLS*VPAAAAFHSSAERTSQEAL
DTVILQSLSCCCGVAHWSKLLAPILGCL*TSTH
FLN*NMQLFHKFILLLSGHNVEWLKVLQD*EFG
FVIDVFSGEGGWDIPDPPSSEGPMSGLTIGSKAM
FTLT*CRVWKHWAGYTFLFLRFILRLCRHGQPL
IRASRVASCLS*RY*WVLFSVSLFCNILFPSFM
GRLSLSSPSMPFYPRLPKCSLLRNSVSSVLHSEK
RG*MMVWGVGVFF*YRGFLAVRKQPHVRHD*AV
KSSWTCCLKIKLICLRHLS*VGGNY*SSANNHS
IGSDAWKGGW*MKKLRRATTFFFPENKFG*NSC

Frame 1

FSQQPPESHHYHGL*VRKLRLGW*SHLPQDEQL
VDHMRGGTGCGPGGLVLVDLFF**FFCFVP*SD
DAGVLVGETGQPAPANQTSTFGSSCDWAWPRDQD
H**EMFSTASTVESRV*QGICQGKAEFEEEAKN
PGLETHRKRKRSGNSDSIERDAAQEAEAGTGLE
PGSNSGQCSVPLKKGKDAPIKKESLGHWSQGLKI
SMQDPKMQVYKDEQVVVIKDKYPKARYHWLVLP
WTSISSLKAVAREHLELLKHMHTVGEKVIVDFA
GSSKLRFRLGYHAIPSMSHVHLHVISQDFDSPCL
KNKKHWNSFNTEYFLESQAVIEMVQEAGRVTVR
DGMPELLKLPLRCHECQQLLPSIPQLKEHLRKH
WTQ*FCRA*AAAVVWPTGANCWHLFWVACELLLI
S*IKTCSFFTNLFYY*VATM*SGSKYFRIRNLG
LS*MYSLVRVAGIYLTHHLQKDPCQV*PLGAKP
CSH*PNAEYGSIGLVIHFCFLDLSSASVGMDNL*
SEHLEWPLVYPEDTDGSCFLLVCFVIFFSLPSW
GGLVCPVLPCPSIPDYLNVPFSGILSHQFFTVR
KEAR*WCGGLEFSSNTEGSWL*GNSHMFVMIEL*
SLLGPVV*K*S*FV*GISLK*VETIEVQLTITA
*VLMHGKVVGE*KSCVEPLLSFSLRINLDKTVV

Frame 2

FHNSLLSPIIIMVYR*GN*D*GGEVTCLKMNSW
*ITCVVAPGVALEDSC*WICFSSDSFVLSLRVM
MRVCWLVRQDSRHQRIRLPHLEAVVIGRGPETK
ITDKKCSRQQVQLKAECNKGYVKVKQSLRKRQRT
LAWKHTGRERDQATVIL*KGMLLRKLRLGQGWN
LGATLANALCP*RREKMHLSKRNPWATGVKA*R
FLCRTPKCRFTKMSRWW**RINTQRPVTIGWSYR
GPPFPV*RLWPGNTLNSLSICTLWGKR*L*ILL
GPANSASDWATTPFRV*AMYIFM*SARILILLA
LKTKNIGILSIQNTS*NHKL*SRWYKRLVE*LSE
MGCLSS*SCPFVVMSASSCCLPFLS*KNISGST
GHSDSAEPELLLWCGPLEQTAGTYSGLLVNFYS
FPKLKHAAFSQIYSIIEWPQCRVAQSTSGLGIWV
CHRCILW*GWLGYT*PTIFRRTHVRSDHWEQSH
VHTDLMQSMEALGWLYISVS*IYPPPL*AWTTF
NQSI*SGLLFILKILMGLVFC*SVL*YSFPFLHG
EA*FVQSFHALLSQIT*MFPSQEFCLISSSQ*E
KRLDDGVGGWSFLLIPRVPGCEETATCSS*LSC
EVFLDLLSENKVNLFEASLLSRWKLLKFS*QSQH
RF*CMERWLVNEKVA*SHYFLFP*E*IWIKQL

Sequence NM_175071

AGCGCCGGAAGCGGTGAGGCACAGATGAGTAACGTGAATTTGTCCGTCTCCGACTTCTGGAGAGTGATGATGCGGGTGTGCTGGTTGGTGAGACAGGACA
GCCGGCACCAGCGAATCAGACTTCCACATTTGGAAGCAGTTGTGATTGGGCGTGGCCCAGAGACCAAGATCACTGATAAGAAATGTTCTCGACAGCAAGT
ACAGTTGAAAGCAGAGTGTAACAAGGGATATGTCAAGGTAAAGCAGAGTTTGAGGAAGAGGCAAAGAACCCTGGCCTGGAAACACACAGGAAGAGAAAGA
GATCAGGCAACAGTGATTCTATAGAAAGGGATGCTGCTCAGGAAGCTGAGGCTGGGACAGGGCTGGAACCTGGGAGCAACTCTGGCCAATGCTCTGTGCC
CCTAAAGAAGGGAAAAGATGCACCTATCAAAAAGGAATCCCTGGGCCACTGGAGTCAAGGCTTGAAGATTTCTATGCAGGACCCCAAAATGCAGGTTTAC
AAAGATGAGCAGGTGGTGGTGATAAAGGATAAATACCCAAAGGCCCGTTACCATTGGCTGGTCTTACCGTGGACCTCCATTTCCAGTCTGAAGGCTGTGG
CCAGGGAACACCTTGAACTCCTTAAGCATATGCACACTGTGGGGGAAAAGGTGATTGTAGATTTTGCTGGGTCCAGCAAACTCCGCTTCCGATTGGGCTA
CCACGCCATTCCGAGTATGAGCCATGTACATCTTCATGTGATCAGCCAGGATTTTGATTCTCCTTGCCTTAAAAACAAAAAACATTGGAATTCTTTCAAT
ACAGAATACTTCCTAGAATCACAAGCTGTGATCGAGATGGTACAAGAGGCTGGTAGAGTAACTGTCCGAGATGGGATGCCTGAGCTCTTGAAGCTGCCCC
TTCGTTGTCATGAGTGCCAGCAGCTGCTGCCTTCCATTCCTCAGCTGAAAGAACATCTCAGGAAGCACTGGACACAGTGATTCTGCAGAGCCTGAGCTGC
TGCTGTGGTGTGGCCCACTGGAGCAAACTGCTGGCACCTATTCTGGGTTGCTTGTGAACTTCTACTCATTTCCTAAATTAAAACATGCAGCTTTTTCACA
AATTTATTCTATTATTGAGTGGCCACAATGTAGAGTGGCTCAAAGTACTTCAGGATTAGGAATTTGGGTTTGTCATAGATGTATTCTCTGGTGAGGGTGG
CTGGGATATACCTGACCCACCATCTTCAGAAGGACCCATGTCAGGTCTGACCATTGGGAGCAAAGCCATGTTCACACTGACCTAATGCAGAGTATGGAAG
CATTGGGCTGGTTATACATTTCTGTTTCTTAGATTTATCCTCCGCCTCTGTAGGCATGGACAACCTTTAATCAGAGCATCTAGAGTGGCCTCTTGTTTAT
CCTGAAGATACTGATGGGTCTTGTTTTCTGTTAGTCTGTTTTGTAATATTCTTTTCCCTTCCTTCATGGGGAGGCTTAGTTTGTCCAGTCCTTCCATGCC
CTTCTATCCCAGATTACCTAAATGTTCCCTTCTCAGGAATTCTGTCTCATCAGTTCTTCACAGTGAGAAAAGAGGCTAGATGATGGTGTGGGGGGTTGGA
GTTTTCTTCTAATACCGAGGGTTCCTGGCTGTGAGGAAACAGCCACATGTTCGTCATGATTGAGCTGTGAAGTCTTCTTGGACCTGTTGTCTGAAAATAA
AGTTAATTTGTTTGAGGCATCTCTCTTAAGTAGGTGGAAACTATTGAAGTTCAGCTAACAATCACAGCATAGGTTCTGATGCATGGAAAGGTGGTTGGTG
AATGAAAAAGTTGCGTAGAGCCACTACTTTCTTTTTCCCTGAGAATAAATTTGGATAAAACAGTTGTA
  • refseq_APTX.F1 (forward primer)
  • refseq_APTX.R1 (reverse primer)
  • Alternative acceptor

Frame 0

SAGSGEAQMSNVNLSVSDFWRVMMRVCWLVRQD
SRHQRIRLPHLEAVVIGRGPETKITDKKCSRQQV
QLKAECNKGYVKVKQSLRKRQRTLAWKHTGRER
DQATVIL*KGMLLRKLRLGQGWNLGATLANALC
P*RREKMHLSKRNPWATGVKA*RFLCRTPKCRFT
KMSRWW**RINTQRPVTIGWSYRGPPFPV*RLW
PGNTLNSLSICTLWGKR*L*ILLGPANSASDWA
TTPFRV*AMYIFM*SARILILLALKTKNIGILSI
QNTS*NHKL*SRWYKRLVE*LSEMGCLSS*SCP
FVVMSASSCCLPFLS*KNISGSTGHSDSAEPEL
LLWCGPLEQTAGTYSGLLVNFYSFPKLKHAAFSQ
IYSIIEWPQCRVAQSTSGLGIWVCHRCILW*GW
LGYT*PTIFRRTHVRSDHWEQSHVHTDLMQSME
ALGWLYISVS*IYPPPL*AWTTFNQSI*SGLLFI
LKILMGLVFC*SVL*YSFPFLHGEA*FVQSFHA
LLSQIT*MFPSQEFCLISSSQ*EKRLDDGVGGW
SFLLIPRVPGCEETATCSS*LSCEVFLDLLSENK
VNLFEASLLSRWKLLKFS*QSQHRF*CMERWLV
NEKVA*SHYFLFP*E*IWIKQL

Frame 1

APEAVRHR*VT*ICPSPTSGE**CGCAGW*DRT
AGTSESDFHIWKQL*LGVAQRPRSLIRNVLDSK
YS*KQSVTRDMSR*SRV*GRGKEPWPGNTQEEKE
IRQQ*FYRKGCCSGS*GWDRAGTWEQLWPMLCA
PKEGKRCTYQKGIPGPLESRLEDFYAGPQNAGL
QR*AGGGDKG*IPKGPLPLAGLTVDLHFQSEGCG
QGTP*TP*AYAHCGGKGDCRFCWVQQTPLPIGL
PRHSEYEPCTSSCDQPGF*FSLP*KQKTLEFFQ
YRILPRITSCDRDGTRGW*SNCPRWDA*ALEAAP
SLS*VPAAAAFHSSAERTSQEALDTVILQSLSC
CCGVAHWSKLLAPILGCL*TSTHFLN*NMQLFH
KFILLLSGHNVEWLKVLQD*EFGFVIDVFSGEGG
WDIPDPPSSEGPMSGLTIGSKAMFTLT*CRVWK
HWAGYTFLFLRFILRLCRHGQPLIRASRVASCL
S*RY*WVLFSVSLFCNILFPSFMGRLSLSSPSMP
FYPRLPKCSLLRNSVSSVLHSEKRG*MMVWGVG
VFF*YRGFLAVRKQPHVRHD*AVKSSWTCCLKI
KLICLRHLS*VGGNY*SSANNHSIGSDAWKGGW*
MKKLRRATTFFFPENKFG*NSC

Frame 2

RRKR*GTDE*REFVRLRLLESDDAGVLVGETGQ
PAPANQTSTFGSSCDWAWPRDQDH**EMFSTAS
TVESRV*QGICQGKAEFEEEAKNPGLETHRKRK
RSGNSDSIERDAAQEAEAGTGLEPGSNSGQCSVP
LKKGKDAPIKKESLGHWSQGLKISMQDPKMQVY
KDEQVVVIKDKYPKARYHWLVLPWTSISSLKAV
AREHLELLKHMHTVGEKVIVDFAGSSKLRFRLGY
HAIPSMSHVHLHVISQDFDSPCLKNKKHWNSFN
TEYFLESQAVIEMVQEAGRVTVRDGMPELLKLP
LRCHECQQLLPSIPQLKEHLRKHWTQ*FCRA*AA
AVVWPTGANCWHLFWVACELLLIS*IKTCSFFT
NLFYY*VATM*SGSKYFRIRNLGLS*MYSLVRV
AGIYLTHHLQKDPCQV*PLGAKPCSH*PNAEYGS
IGLVIHFCFLDLSSASVGMDNL*SEHLEWPLVY
PEDTDGSCFLLVCFVIFFSLPSWGGLVCPVLPC
PSIPDYLNVPFSGILSHQFFTVRKEAR*WCGGLE
FSSNTEGSWL*GNSHMFVMIEL*SLLGPVV*K*
S*FV*GISLK*VETIEVQLTITA*VLMHGKVVG
E*KSCVEPLLSFSLRINLDKTVV

Sequence NM_175072

AGATTTATTTTTTTTAGATGAGGTGTTGCTGTGTTGCCCAAGCTGGACTTGAACCCCTAGGCTCAAGCAATCCTTCCACCTCAGTCTCCCAAGTAGCAGG
GACTACAGAGTGATGATGCGGGTGTGCTGGTTGGTGAGACAGGACAGCCGGCACCAGCGAATCAGACTTCCACATTTGGAAGCAGTTGTGATTGGGCGTG
GCCCAGAGACCAAGATCACTGATAAGAAATGTTCTCGACAGCAAGTTGAAAGCAGAGTGTAACAAGGGATATGTCAAGGTAAAGCAGGTAGGAGTCAATC
CCACCAGCATTGACTCAGTCGTAATTGGGAAGGACCAAGAGGTGAAGCTGCAGCCTGGCCAGGTTCTCCACATGGTGAATGAACTTTATCCATATATTGT
AG
AGTTTGAGGAAGAGGCAAAGAACCCTGGCCTGGAAACACACAGGAAGAGAAAGAGATCAGGCAACAGTGATTCTATAGAAAGGGATGCTGCTCAGGAA
GCTGAGGCTGGGACAGGGCTGGAACCTGGGAGCAACTCTGGCCAATGCTCTGTGCCCCTAAAGAAGGGAAAAGATGCACCTATCAAAAAGGAATCCCTGG
GCCACTGGAGTCAAGGCTTGAAGATTTCTATGCAGGACCCCAAAATGCAGGTTTACAAAGATGAGCAGGTGGTGGTGATAAAGGATAAATACCCAAAGGC
CCGTTACCATTGGCTGGTCTTACCGTGGACCTCCATTTCCAGTCTGAAGGCTGTGGCCAGGGAACACCTTGAACTCCTTAAGCATATGCACACTGTGGGG
GAAAAGGTGATTGTAGATTTTGCTGGGTCCAGCAAACTCCGCTTCCGATTGGGCTACCACGCCATTCCGAGTATGAGCCATGTACATCTTCATGTGATCA
GCCAGGATTTTGATTCTCCTTGCCTTAAAAACAAAAAACATTGGAATTCTTTCAATACAGAATACTTCCTAGAATCACAAGCTGTGATCGAGATGGTACA
AGAGGCTGGTAGAGTAACTGTCCGAGATGGGATGCCTGAGCTCTTGAAGCTGCCCCTTCGTTGTCATGAGTGCCAGCAGCTGCTGCCTTCCATTCCTCAG
CTGAAAGAACATCTCAGGAAGCACTGGACACAGTGATTCTGCAGAGCCTGAGCTGCTGCTGTGGTGTGGCCCACTGGAGCAAACTGCTGGCACCTATTCT
GGGTTGCTTGTGAACTTCTACTCATTTCCTAAATTAAAACATGCAGCTTTTTCACAAATTTATTCTATTATTGAGTGGCCACAATGTAGAGTGGCTCAAA
GTACTTCAGGATTAGGAATTTGGGTTTGTCATAGATGTATTCTCTGGTGAGGGTGGCTGGGATATACCTGACCCACCATCTTCAGAAGGACCCATGTCAG
GTCTGACCATTGGGAGCAAAGCCATGTTCACACTGACCTAATGCAGAGTATGGAAGCATTGGGCTGGTTATACATTTCTGTTTCTTAGATTTATCCTCCG
CCTCTGTAGGCATGGACAACCTTTAATCAGAGCATCTAGAGTGGCCTCTTGTTTATCCTGAAGATACTGATGGGTCTTGTTTTCTGTTAGTCTGTTTTGT
AATATTCTTTTCCCTTCCTTCATGGGGAGGCTTAGTTTGTCCAGTCCTTCCATGCCCTTCTATCCCAGATTACCTAAATGTTCCCTTCTCAGGAATTCTG
TCTCATCAGTTCTTCACAGTGAGAAAAGAGGCTAGATGATGGTGTGGGGGGTTGGAGTTTTCTTCTAATACCGAGGGTTCCTGGCTGTGAGGAAACAGCC
ACATGTTCGTCATGATTGAGCTGTGAAGTCTTCTTGGACCTGTTGTCTGAAAATAAAGTTAATTTGTTTGAGGCATCTCTCTTAAGTAGGTGGAAACTAT
TGAAGTTCAGCTAACAATCACAGCATAGGTTCTGATGCATGGAAAGGTGGTTGGTGAATGAAAAAGTTGCGTAGAGCCACTACTTTCTTTTTCCCTGAGA
ATAAATTTGGATAAAACAGTTGTA
  • refseq_APTX.F1 (forward primer)
  • refseq_APTX.R1 (reverse primer)
  • Alternative acceptor

Frame 0

RFIFFR*GVAVLPKLDLNP*AQAILPPQSPK*Q
GLQSDDAGVLVGETGQPAPANQTSTFGSSCDWAW
PRDQDH**EMFSTAS*KQSVTRDMSR*SR*ESI
PPALTQS*LGRTKR*SCSLARFSTW*MNFIHIL
*
SLRKRQRTLAWKHTGRERDQATVIL*KGMLLRK
LRLGQGWNLGATLANALCP*RREKMHLSKRNPW
ATGVKA*RFLCRTPKCRFTKMSRWW**RINTQR
PVTIGWSYRGPPFPV*RLWPGNTLNSLSICTLWG
KR*L*ILLGPANSASDWATTPFRV*AMYIFM*S
ARILILLALKTKNIGILSIQNTS*NHKL*SRWY
KRLVE*LSEMGCLSS*SCPFVVMSASSCCLPFLS
*KNISGSTGHSDSAEPELLLWCGPLEQTAGTYS
GLLVNFYSFPKLKHAAFSQIYSIIEWPQCRVAQ
STSGLGIWVCHRCILW*GWLGYT*PTIFRRTHVR
SDHWEQSHVHTDLMQSMEALGWLYISVS*IYPP
PL*AWTTFNQSI*SGLLFILKILMGLVFC*SVL
*YSFPFLHGEA*FVQSFHALLSQIT*MFPSQEFC
LISSSQ*EKRLDDGVGGWSFLLIPRVPGCEETA
TCSS*LSCEVFLDLLSENKVNLFEASLLSRWKL
LKFS*QSQHRF*CMERWLVNEKVA*SHYFLFP*E
*IWIKQL

Frame 1

DLFFLDEVLLCCPSWT*TPRLKQSFHLSLPSSR
DYRVMMRVCWLVRQDSRHQRIRLPHLEAVVIGR
GPETKITDKKCSRQQVESRV*QGICQGKAGRSQS
HQH*LSRNWEGPRGEAAAWPGSPHGE*TLSIYC
R
V*GRGKEPWPGNTQEEKEIRQQ*FYRKGCCSG
S*GWDRAGTWEQLWPMLCAPKEGKRCTYQKGIPG
PLESRLEDFYAGPQNAGLQR*AGGGDKG*IPKG
PLPLAGLTVDLHFQSEGCGQGTP*TP*AYAHCG
GKGDCRFCWVQQTPLPIGLPRHSEYEPCTSSCDQ
PGF*FSLP*KQKTLEFFQYRILPRITSCDRDGT
RGW*SNCPRWDA*ALEAAPSLS*VPAAAAFHSS
AERTSQEALDTVILQSLSCCCGVAHWSKLLAPIL
GCL*TSTHFLN*NMQLFHKFILLLSGHNVEWLK
VLQD*EFGFVIDVFSGEGGWDIPDPPSSEGPMS
GLTIGSKAMFTLT*CRVWKHWAGYTFLFLRFILR
LCRHGQPLIRASRVASCLS*RY*WVLFSVSLFC
NILFPSFMGRLSLSSPSMPFYPRLPKCSLLRNS
VSSVLHSEKRG*MMVWGVGVFF*YRGFLAVRKQP
HVRHD*AVKSSWTCCLKIKLICLRHLS*VGGNY
*SSANNHSIGSDAWKGGW*MKKLRRATTFFFPE
NKFG*NSC

Frame 2

IYFF*MRCCCVAQAGLEPLGSSNPSTSVSQVAG
TTE**CGCAGW*DRTAGTSESDFHIWKQL*LGV
AQRPRSLIRNVLDSKLKAECNKGYVKVKQVGVN
PTSIDSVVIGKDQEVKLQPGQVLHMVNELYPYIV
EFEEEAKNPGLETHRKRKRSGNSDSIERDAAQE
AEAGTGLEPGSNSGQCSVPLKKGKDAPIKKESL
GHWSQGLKISMQDPKMQVYKDEQVVVIKDKYPKA
RYHWLVLPWTSISSLKAVAREHLELLKHMHTVG
EKVIVDFAGSSKLRFRLGYHAIPSMSHVHLHVI
SQDFDSPCLKNKKHWNSFNTEYFLESQAVIEMVQ
EAGRVTVRDGMPELLKLPLRCHECQQLLPSIPQ
LKEHLRKHWTQ*FCRA*AAAVVWPTGANCWHLF
WVACELLLIS*IKTCSFFTNLFYY*VATM*SGSK
YFRIRNLGLS*MYSLVRVAGIYLTHHLQKDPCQ
V*PLGAKPCSH*PNAEYGSIGLVIHFCFLDLSS
ASVGMDNL*SEHLEWPLVYPEDTDGSCFLLVCFV
IFFSLPSWGGLVCPVLPCPSIPDYLNVPFSGIL
SHQFFTVRKEAR*WCGGLEFSSNTEGSWL*GNS
HMFVMIEL*SLLGPVV*K*S*FV*GISLK*VETI
EVQLTITA*VLMHGKVVGE*KSCVEPLLSFSLR
INLDKTVV

Sequence NM_175073

AGCGCCGGAAGCGGTGAGGCACAGATGAGTAACGTGAATTTGTCCGTCTCCGACTTCTGGAGATTTATTTTTTTTAGATGAGGTGTTGCTGTGTTGCCCA
AGCTGGACTTGAACCCCTAGGCTCAAGCAATCCTTCCACCTCAGTCTCCCAAGTAGCAGGGACTACAGAGTGATGATGCGGGTGTGCTGGTTGGTGAGAC
AGGACAGCCGGCACCAGCGAATCAGACTTCCACATTTGGAAGCAGTTGTGATTGGGCGTGGCCCAGAGACCAAGATCACTGATAAGAAATGTTCTCGACA
GCAAGTACAGTTGAAAGCAGAGTGTAACAAGGGATATGTCAAGGTAAAGCAGGTAGGAGTCAATCCCACCAGCATTGACTCAGTCGTAATTGGGAAGGAC
CAAGAGGTGAAGCTGCAGCCTGGCCAGGTTCTCCACATGGTGAATGAACTTTATCCATATATTGTAG
AGTTTGAGGAAGAGGCAAAGAACCCTGGCCTGG
AAACACACAGGAAGAGAAAGAGATCAGGCAACAGTGATTCTATAGAAAGGGATGCTGCTCAGGAAGCTGAGGCTGGGACAGGGCTGGAACCTGGGAGCAA
CTCTGGCCAATGCTCTGT
GCCCCTAAAGAAGGGAAAAGATGCACCTATCAAAAAGGAATCCCTGGGCCACTGGAGTCAAGGCTTGAAGATTTCTATGCAG
GACCCCAAAATGCAGGTTTACAAAGATGAGCAGGTGGTGGTGATAAAGGATAAATACCCAAAGGCCCGTTACCATTGGCTGGTCTTACCGTGGACCTCCA
TTTCCAGTCTGAAGGCTGTGGCCAGGGAACACCTTGAACTCCTTAAGCATATGCACACTGTGGGGGAAAAGGTGATTGTAGATTTTGCTGGGTCCAGCAA
ACTCCGCTTCCGATTGGGCTACCACGCCATTCCGAGTATGAGCCATGTACATCTTCATGTGATCAGCCAGGATTTTGATTCTCCTTGCCTTAAAAACAAA
AAACATTGGAATTCTTTCAATACAGAATACTTCCTAGAATCACAAGCTGTGATCGAGATGGTACAAGAGGCTGGTAGAGTAACTGTCCGAGATGGGATGC
CTGAGCTCTTGAAGCTGCCCCTTCGTTGTCATGAGTGCCAGCAGCTGCTGCCTTCCATTCCTCAGCTGAAAGAACATCTCAGGAAGCACTGGACACAGTG
ATTCTGCAGAGCCTGAGCTGCTGCTGTGGTGTGGCCCACTGGAGCAAACTGCTGGCACCTATTCTGGGTTGCTTGTGAACTTCTACTCATTTCCTAAATT
AAAACATGCAGCTTTTTCACAAATTTATTCTATTATTGAGTGGCCACAATGTAGAGTGGCTCAAAGTACTTCAGGATTAGGAATTTGGGTTTGTCATAGA
TGTATTCTCTGGTGAGGGTGGCTGGGATATACCTGACCCACCATCTTCAGAAGGACCCATGTCAGGTCTGACCATTGGGAGCAAAGCCATGTTCACACTG
ACCTAATGCAGAGTATGGAAGCATTGGGCTGGTTATACATTTCTGTTTCTTAGATTTATCCTCCGCCTCTGTAGGCATGGACAACCTTTAATCAGAGCAT
CTAGAGTGGCCTCTTGTTTATCCTGAAGATACTGATGGGTCTTGTTTTCTGTTAGTCTGTTTTGTAATATTCTTTTCCCTTCCTTCATGGGGAGGCTTAG
TTTGTCCAGTCCTTCCATGCCCTTCTATCCCAGATTACCTAAATGTTCCCTTCTCAGGAATTCTGTCTCATCAGTTCTTCACAGTGAGAAAAGAGGCTAG
ATGATGGTGTGGGGGGTTGGAGTTTTCTTCTAATACCGAGGGTTCCTGGCTGTGAGGAAACAGCCACATGTTCGTCATGATTGAGCTGTGAAGTCTTCTT
GGACCTGTTGTCTGAAAATAAAGTTAATTTGTTTGAGGCATCTCTCTTAAGTAGGTGGAAACTATTGAAGTTCAGCTAACAATCACAGCATAGGTTCTGA
TGCATGGAAAGGTGGTTGGTGAATGAAAAAGTTGCGTAGAGCCACTACTTTCTTTTTCCCTGAGAATAAATTTGGATAAAACAGTTGTA
  • refseq_APTX.F1 (forward primer)
  • refseq_APTX.R1 (reverse primer)
  • Alternative acceptor

Frame 0

SAGSGEAQMSNVNLSVSDFWRFIFFR*GVAVLP
KLDLNP*AQAILPPQSPK*QGLQSDDAGVLVGET
GQPAPANQTSTFGSSCDWAWPRDQDH**EMFST
ASTVESRV*QGICQGKAGRSQSHQH*LSRNWEG
PRGEAAAWPGSPHGE*TLSIYCR
V*GRGKEPWPG
NTQEEKEIRQQ*FYRKGCCSGS*GWDRAGTWEQ
LWPMLC
APKEGKRCTYQKGIPGPLESRLEDFYA
GPQNAGLQR*AGGGDKG*IPKGPLPLAGLTVDLH
FQSEGCGQGTP*TP*AYAHCGGKGDCRFCWVQQ
TPLPIGLPRHSEYEPCTSSCDQPGF*FSLP*KQ
KTLEFFQYRILPRITSCDRDGTRGW*SNCPRWDA
*ALEAAPSLS*VPAAAAFHSSAERTSQEALDTV
ILQSLSCCCGVAHWSKLLAPILGCL*TSTHFLN
*NMQLFHKFILLLSGHNVEWLKVLQD*EFGFVID
VFSGEGGWDIPDPPSSEGPMSGLTIGSKAMFTL
T*CRVWKHWAGYTFLFLRFILRLCRHGQPLIRA
SRVASCLS*RY*WVLFSVSLFCNILFPSFMGRLS
LSSPSMPFYPRLPKCSLLRNSVSSVLHSEKRG*
MMVWGVGVFF*YRGFLAVRKQPHVRHD*AVKSS
WTCCLKIKLICLRHLS*VGGNY*SSANNHSIGSD
AWKGGW*MKKLRRATTFFFPENKFG*NSC

Frame 1

APEAVRHR*VT*ICPSPTSGDLFFLDEVLLCCP
SWT*TPRLKQSFHLSLPSSRDYRVMMRVCWLVR
QDSRHQRIRLPHLEAVVIGRGPETKITDKKCSRQ
QVQLKAECNKGYVKVKQVGVNPTSIDSVVIGKD
QEVKLQPGQVLHMVNELYPYIV
EFEEEAKNPGL
ETHRKRKRSGNSDSIERDAAQEAEAGTGLEPGSN
SGQCSV
PLKKGKDAPIKKESLGHWSQGLKISMQ
DPKMQVYKDEQVVVIKDKYPKARYHWLVLPWTS
ISSLKAVAREHLELLKHMHTVGEKVIVDFAGSSK
LRFRLGYHAIPSMSHVHLHVISQDFDSPCLKNK
KHWNSFNTEYFLESQAVIEMVQEAGRVTVRDGM
PELLKLPLRCHECQQLLPSIPQLKEHLRKHWTQ*
FCRA*AAAVVWPTGANCWHLFWVACELLLIS*I
KTCSFFTNLFYY*VATM*SGSKYFRIRNLGLS*
MYSLVRVAGIYLTHHLQKDPCQV*PLGAKPCSH*
PNAEYGSIGLVIHFCFLDLSSASVGMDNL*SEH
LEWPLVYPEDTDGSCFLLVCFVIFFSLPSWGGL
VCPVLPCPSIPDYLNVPFSGILSHQFFTVRKEAR
*WCGGLEFSSNTEGSWL*GNSHMFVMIEL*SLL
GPVV*K*S*FV*GISLK*VETIEVQLTITA*VL
MHGKVVGE*KSCVEPLLSFSLRINLDKTVV

Frame 2

RRKR*GTDE*REFVRLRLLEIYFF*MRCCCVAQ
AGLEPLGSSNPSTSVSQVAGTTE**CGCAGW*D
RTAGTSESDFHIWKQL*LGVAQRPRSLIRNVLD
SKYS*KQSVTRDMSR*SR*ESIPPALTQS*LGRT
KR*SCSLARFSTW*MNFIHIL*
SLRKRQRTLAW
KHTGRERDQATVIL*KGMLLRKLRLGQGWNLGA
TLANAL
CP*RREKMHLSKRNPWATGVKA*RFLCR
TPKCRFTKMSRWW**RINTQRPVTIGWSYRGPP
FPV*RLWPGNTLNSLSICTLWGKR*L*ILLGPA
NSASDWATTPFRV*AMYIFM*SARILILLALKTK
NIGILSIQNTS*NHKL*SRWYKRLVE*LSEMGC
LSS*SCPFVVMSASSCCLPFLS*KNISGSTGHS
DSAEPELLLWCGPLEQTAGTYSGLLVNFYSFPKL
KHAAFSQIYSIIEWPQCRVAQSTSGLGIWVCHR
CILW*GWLGYT*PTIFRRTHVRSDHWEQSHVHT
DLMQSMEALGWLYISVS*IYPPPL*AWTTFNQSI
*SGLLFILKILMGLVFC*SVL*YSFPFLHGEA*
FVQSFHALLSQIT*MFPSQEFCLISSSQ*EKRL
DDGVGGWSFLLIPRVPGCEETATCSS*LSCEVFL
DLLSENKVNLFEASLLSRWKLLKFS*QSQHRF*
CMERWLVNEKVA*SHYFLFP*E*IWIKQL

Sequence NM_175069

AGCGCCGGAAGCGGTGAGGCACAGATGAGTAACGTGAATTTGTCCGTCTCCGACTTCTGGAGAGTGATGATGCGGGTGTGCTGGTTGGTGAGACAGGACA
GCCGGCACCAGCGAATCAGACTTCCACATTTGGAAGCAGTTGTGATTGGGCGTGGCCCAGAGACCAAGATCACTGATAAGAAATGTTCTCGACAGCAAGT
ACAGTTGAAAGCAGAGTGTAACAAGGGATATGTCAAGGTAAAGCAGGTAGGAGTCAATCCCACCAGCATTGACTCAGTCGTAATTGGGAAGGACCAAGAG
GTGAAGCTGCAGCCTGGCCAGGTTCTCCACATGGTGAATGAACTTTATCCATATATTGTAG
AGTTTGAGGAAGAGGCAAAGAACCCTGGCCTGGAAACAC
ACAGGAAGAGAAAGAGATCAGGCAACAGTGATTCTATAGAAAGGGATGCTGCTCAGGAAGCTGAGGCTGGGACAGGGCTGGAACCTGGGAGCAACTCTGG
CCAATGCTCTGT
GCCCCTAAAGAAGGGAAAAGATGCACCTATCAAAAAGGAATCCCTGGGCCACTGGAGTCAAGGCTTGAAGATTTCTATGCAGGACCCC
AAAATGCAGGTTTACAAAGATGAGCAGGTGGTGGTGATAAAGGATAAATACCCAAAGGCCCGTTACCATTGGCTGGTCTTACCGTGGACCTCCATTTCCA
GTCTGAAGGCTGTGGCCAGGGAACACCTTGAACTCCTTAAGCATATGCACACTGTGGGGGAAAAGGTGATTGTAGATTTTGCTGGGTCCAGCAAACTCCG
CTTCCGATTGGGCTACCACGCCATTCCGAGTATGAGCCATGTACATCTTCATGTGATCAGCCAGGATTTTGATTCTCCTTGCCTTAAAAACAAAAAACAT
TGGAATTCTTTCAATACAGAATACTTCCTAGAATCACAAGAGTAGCTGGGATTTGACTTTTCTTCCTTGCCTGCAGCTGTGATCGAGATGGTACAAGAGG
CTGGTAGAGTAACTGTCCGAGATGGGATGCCTGAGCTCTTGAAGCTGCCCCTTCGTTGTCATGAGTGCCAGCAGCTGCTGCCTTCCATTCCTCAGCTGAA
AGAACATCTCAGGAAGCACTGGACACAGTGATTCTGCAGAGCCTGAGCTGCTGCTGTGGTGTGGCCCACTGGAGCAAACTGCTGGCACCTATTCTGGGTT
GCTTGTGAACTTCTACTCATTTCCTAAATTAAAACATGCAGCTTTTTCACAAATTTATTCTATTATTGAGTGGCCACAATGTAGAGTGGCTCAAAGTACT
TCAGGATTAGGAATTTGGGTTTGTCATAGATGTATTCTCTGGTGAGGGTGGCTGGGATATACCTGACCCACCATCTTCAGAAGGACCCATGTCAGGTCTG
ACCATTGGGAGCAAAGCCATGTTCACACTGACCTAATGCAGAGTATGGAAGCATTGGGCTGGTTATACATTTCTGTTTCTTAGATTTATCCTCCGCCTCT
GTAGGCATGGACAACCTTTAATCAGAGCATCTAGAGTGGCCTCTTGTTTATCCTGAAGATACTGATGGGTCTTGTTTTCTGTTAGTCTGTTTTGTAATAT
TCTTTTCCCTTCCTTCATGGGGAGGCTTAGTTTGTCCAGTCCTTCCATGCCCTTCTATCCCAGATTACCTAAATGTTCCCTTCTCAGGAATTCTGTCTCA
TCAGTTCTTCACAGTGAGAAAAGAGGCTAGATGATGGTGTGGGGGGTTGGAGTTTTCTTCTAATACCGAGGGTTCCTGGCTGTGAGGAAACAGCCACATG
TTCGTCATGATTGAGCTGTGAAGTCTTCTTGGACCTGTTGTCTGAAAATAAAGTTAATTTGTTTGAGGCATCTCTCTTAAGTAGGTGGAAACTATTGAAG
TTCAGCTAACAATCACAGCATAGGTTCTGATGCATGGAAAGGTGGTTGGTGAATGAAAAAGTTGCGTAGAGCCACTACTTTCTTTTTCCCTGAGAATAAA
TTTGGATAAAACAGTTGTA
  • refseq_APTX.F1 (forward primer)
  • refseq_APTX.R1 (reverse primer)
  • Alternative acceptor

Frame 0

SAGSGEAQMSNVNLSVSDFWRVMMRVCWLVRQD
SRHQRIRLPHLEAVVIGRGPETKITDKKCSRQQV
QLKAECNKGYVKVKQVGVNPTSIDSVVIGKDQE
VKLQPGQVLHMVNELYPYIV
EFEEEAKNPGLET
HRKRKRSGNSDSIERDAAQEAEAGTGLEPGSNSG
QCSV
PLKKGKDAPIKKESLGHWSQGLKISMQDP
KMQVYKDEQVVVIKDKYPKARYHWLVLPWTSIS
SLKAVAREHLELLKHMHTVGEKVIVDFAGSSKLR
FRLGYHAIPSMSHVHLHVISQDFDSPCLKNKKH
WNSFNTEYFLESQE*LGFDFSSLPAAVIEMVQE
AGRVTVRDGMPELLKLPLRCHECQQLLPSIPQLK
EHLRKHWTQ*FCRA*AAAVVWPTGANCWHLFWV
ACELLLIS*IKTCSFFTNLFYY*VATM*SGSKY
FRIRNLGLS*MYSLVRVAGIYLTHHLQKDPCQV*
PLGAKPCSH*PNAEYGSIGLVIHFCFLDLSSAS
VGMDNL*SEHLEWPLVYPEDTDGSCFLLVCFVI
FFSLPSWGGLVCPVLPCPSIPDYLNVPFSGILSH
QFFTVRKEAR*WCGGLEFSSNTEGSWL*GNSHM
FVMIEL*SLLGPVV*K*S*FV*GISLK*VETIE
VQLTITA*VLMHGKVVGE*KSCVEPLLSFSLRIN
LDKTVV

Frame 1

APEAVRHR*VT*ICPSPTSGE**CGCAGW*DRT
AGTSESDFHIWKQL*LGVAQRPRSLIRNVLDSK
YS*KQSVTRDMSR*SR*ESIPPALTQS*LGRTKR
*SCSLARFSTW*MNFIHIL*
SLRKRQRTLAWKH
TGRERDQATVIL*KGMLLRKLRLGQGWNLGATL
ANAL
CP*RREKMHLSKRNPWATGVKA*RFLCRTP
KCRFTKMSRWW**RINTQRPVTIGWSYRGPPFP
V*RLWPGNTLNSLSICTLWGKR*L*ILLGPANS
ASDWATTPFRV*AMYIFM*SARILILLALKTKNI
GILSIQNTS*NHKSSWDLTFLPCLQL*SRWYKR
LVE*LSEMGCLSS*SCPFVVMSASSCCLPFLS*
KNISGSTGHSDSAEPELLLWCGPLEQTAGTYSGL
LVNFYSFPKLKHAAFSQIYSIIEWPQCRVAQST
SGLGIWVCHRCILW*GWLGYT*PTIFRRTHVRS
DHWEQSHVHTDLMQSMEALGWLYISVS*IYPPPL
*AWTTFNQSI*SGLLFILKILMGLVFC*SVL*Y
SFPFLHGEA*FVQSFHALLSQIT*MFPSQEFCL
ISSSQ*EKRLDDGVGGWSFLLIPRVPGCEETATC
SS*LSCEVFLDLLSENKVNLFEASLLSRWKLLK
FS*QSQHRF*CMERWLVNEKVA*SHYFLFP*E*
IWIKQL

Frame 2

RRKR*GTDE*REFVRLRLLESDDAGVLVGETGQ
PAPANQTSTFGSSCDWAWPRDQDH**EMFSTAS
TVESRV*QGICQGKAGRSQSHQH*LSRNWEGPR
GEAAAWPGSPHGE*TLSIYCR
V*GRGKEPWPGNT
QEEKEIRQQ*FYRKGCCSGS*GWDRAGTWEQLW
PMLC
APKEGKRCTYQKGIPGPLESRLEDFYAGP
QNAGLQR*AGGGDKG*IPKGPLPLAGLTVDLHFQ
SEGCGQGTP*TP*AYAHCGGKGDCRFCWVQQTP
LPIGLPRHSEYEPCTSSCDQPGF*FSLP*KQKT
LEFFQYRILPRITRVAGI*LFFLACSCDRDGTRG
W*SNCPRWDA*ALEAAPSLS*VPAAAAFHSSAE
RTSQEALDTVILQSLSCCCGVAHWSKLLAPILG
CL*TSTHFLN*NMQLFHKFILLLSGHNVEWLKVL
QD*EFGFVIDVFSGEGGWDIPDPPSSEGPMSGL
TIGSKAMFTLT*CRVWKHWAGYTFLFLRFILRL
CRHGQPLIRASRVASCLS*RY*WVLFSVSLFCNI
LFPSFMGRLSLSSPSMPFYPRLPKCSLLRNSVS
SVLHSEKRG*MMVWGVGVFF*YRGFLAVRKQPH
VRHD*AVKSSWTCCLKIKLICLRHLS*VGGNY*S
SANNHSIGSDAWKGGW*MKKLRRATTFFFPENK
FG*NSC

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