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Sequences

Sequence NM_004830

GCTGTTGGCTGGGCTCGGGAGGGAACGCTCCAGGTAAAGCGCTGGGGGAGGAAGCGACGCCGAGGAGCTACGGTTTCCTCCAGAGGTCTCCGCCCCTCTG
CCCCTATATTCCCAGAGCTCGAGTCTGATCCGGGCCTTGCCGGGCACCCTGGAAAGGCGGGGGTGATAGTACAGATGGAGACGCAACTGCAGAGCATTTT
CGAAGAGGTGGTGAAAACGGAAGTTATAGAAGAGGCTTTTCCTGGCATGTTTATGGATACTCCTGAAGATGAGAAAACAAAACTAATTAGCTGTTTGGGG
GCCTTCAGACAGTTTTGGGGTGGTCTTTCTCAGGAGTCTCATGAACAGTGTATCCAGTGGATTGTTAAGTTTATTCATGGTCAGCATAGTCCTAAAAGAA
TTTCTTTTCTTTATGACTGCTTAGCAATGGCAGTTGAGACTGGTCTCCTTCCACCCAGGCTGGTTTGTGAATCCCTGATAAACTCTGACACTCTTGAGTG
GGAAAGAACACAGCTTTGGGCCTTAACATTTAAACTGGTTCGGAAAATAATTGGGGGAGTGGATTACAAGGGTGTTCGAGATCTCTTAAAAGTGATTTTG
GAGAAGATTTTGACAATTCCTAATACAGTGAGCTCTGCTGTTGTACAGCAGCTTCTGGCAGCAAGAGAGGTTATAGCATATATCTTGGAAAGAAATGCCT
GCTTATTACCAGCCTATTTTGCAGTCACTGAGATCAGGAAACTGTATCCTGAAGGCAAACTTCCACACTGGTTACTTGGAAACCTAGTATCAGACTTTGT
GGATACCTTCAGGCCCACAGCAAGGATAAACTCCATTTGTGGTCGCTGTAGTCTTCTGCCAGTTGTAAATAATTCGGGTGCCATTTGTAATTCATGGAAA
CTGGATCCTGCTACTCTTCGTTTTCCTTTGAAAGGCCTTTTGCCATATGATAAGGATCTGTTTGAACCACAGACTGCTTTGTTGAGATATGTATTGGAGC
AGCCTTATTCCAGGGATATGGTCTGCAATATGCTAGGTTTAAATAAGCAGCACAAGCAGCGCTGCCCTGTGCTGGAGGACCAGTTGGTGGATCTGGTTGT
TTATGCCATGGAGCGATCTGAGACCGAGGAGAAGTTTGACGATGGGGGAACAAGCCAACTCCTGTGGCAGCATCTCTCAAGTCAGCTCATTTTCTTTGTG
CTTTTCCAGTTTGCAAGTTTTCCACATATGGTGCTTTCTCTTCATCAGAAGTTAGCAGGGCGAGGACTGATTAAAGGCAGAGATCATCTTATGTGGGTTC
TCCTGCAATTCATTTCTGGAAGTATTCAGAAAAATGCACTAGCTGATTTTCTCCCTGTGATGAAGCTCTTCGACTTGCTATACCCAGAAAAAGAATATAT
CCCAGTTCCTGATATTAACAAACCCCAGTCAACCCATGCCTTTGCAATGACCTGTATTTGGATTCATCTCAATAGAAAAGCTCAAAATGACAACTCCAAG
CTACAGATTCCAATACCTCATTCCCTAAGACTTCACCATGAGTTCCTGCAGCAGAGTCTAAGAAATAAAAGTTTACAGATGAATGACTATAAGATTGCTC
TATTGTGTAATGCATACTCTACAAATTCAGAATGTTTTACATTACCCATGGGAGCTCTGGTAGAAACTATTTATGGAAATGGAATTATGAGGATACCTCT
CCCTGGAACAAACTGTATGGCTTCAGGATCTATTACCCCCTTACCTATGAACCTCCTGGATTCACTGACAGTTCATGCCAAAATGAGCCTTATTCACAGC
ATTGCAACCAGGGTGATAAAACTTGCTCATGCAAAGTCCAGTGTGGCCTTGGCTCCAGCCCTAGTGGAAACTTACAGTCGTTTATTGGTCTATATGGAAA
TAGAGTCTTTGGGCATCAAAGGATTTATCAGTCAGCTTTTGCCAACTGTTTTCAAATCACATGCATGGGGGATCTTACACACACTCCTTGAGATGTTTAG
CTACCGGATGCATCATATTCAGCCTCATTACAGAGTTCAGCTCCTGAGTCATCTTCATACTTTGGCTGCAGTTGCACAAACAAACCAGAACCAGCTCCAT
CTTTGTGTCGAGAGCACTGCTCTCAGGCTTATAACAGCATTAGGTAGCTCAGAGGTACAACCGCAGTTTACACGCTTCCTTAGTGATCCCAAAACAGTGC
TCTCAGCAGAATCTGAAGAACTGAACCGAGCCTTGATATTGACCTTGGCTAGAGCAACTCATGTAACAGATTTTTTTACAGGCTCTGATTCAATTCAGGG
AACTTGGTGTAAAGACATACTTCAGACCATCATGAGTTTCACTCCTCATAATTGGGCTTCACACACCCTGAGCTGTTTTCCAGGCCCACTACAGGCATTC
TTCAAACAAAATAATGTGCCTCAGGAAAGCCGTTTTAATCTGAAAAAAAATGTGGAGGAGGAGTATAGGAAGTGGAAGTCAATGAGCAACGAAAACGACA
TTATTACCCACTTCTCTATGCAGGGCTCCCCTCCTCTCTTTCTTTGTCTTCTCTGGAAAATGCTCTTGGAAACAGATCATATTAATCAGATTGGCTATAG
AGTATTAGAGAGAATTGGAGCCAGGGCCTTGGTAGCCCATGTGAGGACATTTGCAGATTTCCTGGTATATGAGTTTTCTACATCAGCAGGGGGTCAGCAA
CTCAATAAATGCATTGAAATTCTTAATGACATGGTATGGAAGTATAACATTGTTACACTGGACAGATTAATTCTCTGCCTGGCCATGCGTAGTCACGAAG
GAAATGAAGCCCAGGTTTGTTATTTCATAATTCAGTTGCTGTTACTCAAACCAAACGATTTTAGAAATCGAGTAAGTGACTTTGTGAAGGAAAATTCCCC
AGAGCACTGGTTACAGAATGACTGGCACACCAAGCACATGAATTATCACAAGAAATATCCAGAGAAGTTGTATTTTGAGGGCCTCGCGGAACAGGTGGAT
CCTCCTGTACAGATCCAGTCTCCCTATCTGCCCATCTATTTTGGGAATGTGTGTCTTCGATTCCTTCCAGTATTTGATATAGTAATCCACAGATTTTTAG
AGTTGCTTCCGGTATCCAAATCACTGGAGACTCTACTGGATCATCTAGGAGGCTTATATAAATTTCATGATCGTCCAGTGACTTATCTGTATAACACTCT
GCACTATTATGAAATGCACCTGAGAGACCGCGCATTTCTCAAACGAAAACTCGTCCATGCGATCATTGGCTCTCTGAAGGATAATCGACCGCAGGGCTGG
TGTCTAAGTGACACTTACCTGAAATGCGCTATGAATGCACGAGAGGAAAATCCTTGGGTTCCAGATGACACCTACTATTGCAGATTGATTGGCAGACTAG
TCGATACGATGGCTGGCAAATCTCCTGGTCCCTTTCCAAACTGTGACTGGAGATTCAATGAGTTTCCCAACCCAGCTGCCCATGCTCTCCATGTTACTTG
TGTGGAGCTCATGGCCTTGGCAGTTTCAGGCAAAGAAGTTGGGAATGCCCTTCTAAATGTTGTCCTAAAAAGTCAGCCTTTAGTGCCAAGAGAGAACATT
ACAGCATGGATGAATGCAATTGGTTTGATCATCACTGCCCTACCAGAGCCATATTGGATTGTTCTTCATGATCGAATTGTGAGTGTCATCAGCAGCCCCA
GCTTGACGTCTGAAACAGAGTGGGTTGGCTATCCATTCCGCCTCTTTGATTTCACTGCCTGTCATCAGTCCTACTCTGAGATGAGTTGTAGCTATACGTT
AGCTCTTGCACATGCTGTGTGGCACCATTCTAGCATCGGACAACTTTCTCTCATTCCAAAGTTTCTTACTGAAGTACTTCTTCCTATAGTGAAGACCGAA
TTCCAGTTGCTTTATGTATACCATCTTGTTGGACCATTTTTACAAAGATTTCAGCAAGAGAGAACTCGTTGTATGATAGAGATTGGTGTGGCGTTTTATG
ACATGCTGCTGAATGTTGACCAGTGTAGCACCCATTTAAATTACATGGATCCCATCTGTGACTTCCTCTATCACATGAAGTATATGTTTACTGGTGACAG
CGTGAAAGAGCAAGTAGAGAAGATTATCTGTAACTTAAAACCAGCTTTAAAACTTCGTCTTCGATTCATCACACACATTAGCAAGATGGAGCCAGCTGCA
GTGCCTCCACAAGCCATGAACAGTGGGTCTCCAGCACCTCAGTCTAATCAGGTGCCCGTGTCTTTACCAGTAACTCAGTGAAGCCAGACTGTACTGTGGA
GAAAGTAGAAATATATCCGTCTTTGAGAGTGGACTCAAACCTTTTAAATCTGAATGCGATCACACTGTAGTGATATAGTAGAAGCAGTGATGATATTCAG
ATTGTTTTATTTTGATTCAAATGATGAATCTGTTGACACCTTATAACTCCCATCTCCCTATCTATATTTTTGTCTCTAAGAGATCAAGTAAGATACATGT
TGTTCATATTCATAACAACTTATTAGAGCTATGTAGGAACATAGAGGTATCTGTGATCAAATGACTTGTCATTGGCACTTTCAGTCTTTCCTATTCTAGC
CGTTGTTTTTGTTTGTATTTTTTATTATTAATGACATATTTAGTCTTCAGATATATTATTTTAAAGGCACCACACATGTAAAACAGTTATTACAAATATT
TTATACATAAATCTCTTGGTAGATTTTCAGAATAATAGAGATTTATATCAAGAAAAGGAATCTAGATTTTGTTGCATTGTAGGAGCAAACAAGGCTTCAT
ATTCTTTTTAAAATGACTTGAAATTTTATTATAAATTTCCATAGTTTCTATCAAGGTCAACTGGCAGTTTTATTACCTTTGCTATGGACTTGAACTTACT
GTATAAAATTAGTTGGAAGCCATGTAAGAAAATAACATCTGAAAATTCTTGAACTTAAAATCATGCTTAAATGGTTGATTCTCCAATTTCTTTTTTTAAA
AATAAGTTAATTGCTTTTGCAGGTTGACACTCTCACCTGACAGATGATGTAATTCTTCAATTTTTATAATCTTAAAATTTTTAAATTTTATATTTGTAAA
TACAGTACACATTTTATTTCTTGGATTTTGAGAGACATTGTTAATTTTGGGGGAATTGGCATTGCGAAAGACTTGAAAACTAATGAGTAAAGTCTGCTGA
ATGAATAAACCGTGTGATTGTTTCCCG
  • refseq_CRSP3.F1 (forward primer)
  • refseq_CRSP3.R1 (reverse primer)
  • Single exon skipping

Frame 0

AVGWAREGTLQVKRWGRKRRRGATVSSRGLRPS
APIFPELESDPGLAGHPGKAGVIVQMETQLQSIF
EEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLG
AFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKR
ISFLYDCLAMAVETGLLPPRLVCESLINSDTLEW
ERTQLWALTFKLVRKIIGGVDYKGVRDLLKVIL
EKILTIPNTVSSAVVQQLLAAREVIAYILERNA
CLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFV
DTFRPTARINSICGRCSLLPVVNNSGAICNSWK
LDPATLRFPLKGLLPYDKDLFEPQTALLRYVLE
QPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVV
YAMERSETEEKFDDGGTSQLLWQHLSSQLIFFV
LFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWV
LLQFISGSIQKNALADFLPVMKLFDLLYPEKEYI
PVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSK
LQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIA
LLCNAYSTNSECFTLPMGALVETIYGNGIMRIPL
PGTNCMASGSITPLPMNLLDSLTVHAKMSLIHS
IATRVIKLAHAKSSVALAPALVETYSRLLVYME
IESLGIKGFISQLLPTVFKSHAWGILHTLLEMFS
YRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLH
LCVESTALRLITALGSSEVQPQFTRFLSDPKTV
LSAESEELNRALILTLARATHVTDFFTGSDSIQG
TWCKDILQTIMSFTPHNWASHTLSCFPGPLQAF
FKQNNVPQESRFNLKKNVEEEYRKWKSMSNEND
IITHFSMQGSPPLFLCLLWKMLLETDHINQIGYR
VLERIGARALVAHVRTFADFLVYEFSTSAGGQQ
LNKCIEILNDMVWKYNIVTLDRLILCLAMRSHE
GNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSP
EHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVD
PPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFL
ELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTL
HYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGW
CLSDTYLKCAMNAREENPWVPDDTYYCRLIGRL
VDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTC
VELMALAVSGKEVGNALLNVVLKSQPLVPRENI
TAWMNAIGLIITALPEPYWIVLHDRIVSVISSP
SLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTL
ALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTE
FQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFY
DMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDS
VKEQVEKIICNLKPALKLRLRFITHISKMEPAA
VPPQAMNSGSPAPQSNQVPVSLPVTQ*SQTVLW
RK*KYIRL*EWTQTF*I*MRSHCSDIVEAVMIFR
LFYFDSNDESVDTL*LPSPYLYFCL*EIK*DTC
CSYS*QLIRAM*EHRGICDQMTCHWHFQSFLF*
PLFLFVFFIINDIFSLQIYYFKGTTHVKQLLQIF
YT*ISW*IFRIIEIYIKKRNLDFVAL*EQTRLH
ILFKMT*NFIINFHSFYQGQLAVLLPLLWT*TY
CIKLVGSHVRK*HLKILELKIMLKWLILQFLFLK
IS*LLLQVDTLT*QMM*FFNFYNLKIFKFYICK
YSTHFISWILRDIVNFGGIGIAKDLKTNE*SLL
NE*TV*LFP

Frame 1

LLAGLGRERSR*SAGGGSDAEELRFPPEVSAPL
PLYSQSSSLIRALPGTLERRG**YRWRRNCRAF
SKRW*KRKL*KRLFLACLWILLKMRKQN*LAVWG
PSDSFGVVFLRSLMNSVSSGLLSLFMVSIVLKE
FLFFMTA*QWQLRLVSFHPGWFVNP**TLTLLS
GKEHSFGP*HLNWFGK*LGEWITRVFEIS*K*FW
RRF*QFLIQ*ALLLYSSFWQQERL*HISWKEMP
AYYQPILQSLRSGNCILKANFHTGYLET*YQTL
WIPSGPQQG*TPFVVAVVFCQL*IIRVPFVIHGN
WILLLFVFL*KAFCHMIRICLNHRLLC*DMYWS
SLIPGIWSAIC*V*ISSTSSAALCWRTSWWIWL
FMPWSDLRPRRSLTMGEQANSCGSISQVSSFSLC
FSSLQVFHIWCFLFIRS*QGED*LKAEIILCGF
SCNSFLEVFRKMH*LIFSL**SSSTCYTQKKNI
SQFLILTNPSQPMPLQ*PVFGFISIEKLKMTTPS
YRFQYLIP*DFTMSSCSRV*EIKVYR*MTIRLL
YCVMHTLQIQNVLHYPWELW*KLFMEMEL*GYL
SLEQTVWLQDLLPPYL*TSWIH*QFMPK*ALFTA
LQPG**NLLMQSPVWPWLQP*WKLTVVYWSIWK
*SLWASKDLSVSFCQLFSNHMHGGSYTHSLRCL
ATGCIIFSLITEFSS*VIFILWLQLHKQTRTSSI
FVSRALLSGL*QH*VAQRYNRSLHASLVIPKQC
SQQNLKN*TEP*Y*PWLEQLM*QIFLQALIQFR
ELGVKTYFRPS*VSLLIIGLHTP*AVFQAHYRHS
SNKIMCLRKAVLI*KKMWRRSIGSGSQ*ATKTT
LLPTSLCRAPLLSFFVFSGKCSWKQIILIRLAI
EY*RELEPGPW*PM*GHLQISWYMSFLHQQGVSN
SINALKFLMTWYGSITLLHWTD*FSAWPCVVTK
EMKPRFVIS*FSCCYSNQTILEIE*VTL*RKIP
QSTGYRMTGTPST*IITRNIQRSCILRASRNRWI
LLYRSSLPICPSILGMCVFDSFQYLI**STDF*
SCFRYPNHWRLYWII*EAYINFMIVQ*LICITL
CTIMKCT*ETAHFSNENSSMRSLAL*RIIDRRAG
V*VTLT*NAL*MHERKILGFQMTPTIAD*LAD*
SIRWLANLLVPFQTVTGDSMSFPTQLPMLSMLL
VWSSWPWQFQAKKLGMPF*MLS*KVSL*CQERTL
QHG*MQLV*SSLPYQSHIGLFFMIEL*VSSAAP
A*RLKQSGLAIHSASLISLPVISPTLR*VVAIR
*LLHMLCGTILASDNFLSFQSFLLKYFFL**RPN
SSCFMYTILLDHFYKDFSKRELVV**RLVWRFM
TCC*MLTSVAPI*ITWIPSVTSSIT*SICLLVT
A*KSK*RRLSVT*NQL*NFVFDSSHTLARWSQLQ
CLHKP*TVGLQHLSLIRCPCLYQ*LSEARLYCG
ESRNISVFESGLKPFKSECDHTVVI**KQ**YS
DCFILIQMMNLLTPYNSHLPIYIFVSKRSSKIHV
VHIHNNLLELCRNIEVSVIK*LVIGTFSLSYSS
RCFCLYFLLLMTYLVFRYIILKAPHM*NSYYKY
FIHKSLGRFSE**RFISRKGI*ILLHCRSKQGFI
FFLK*LEILL*ISIVSIKVNWQFYYLCYGLELT
V*N*LEAM*ENNI*KFLNLKSCLNG*FSNFFF*
K*VNCFCRLTLSPDR*CNSSIFIILKFLNFIFVN
TVHILFLGF*ETLLILGELALRKT*KLMSKVC*
MNKPCDCFP

Frame 2

CWLGSGGNAPGKALGEEATPRSYGFLQRSPPLC
PYIPRARV*SGPCRAPWKGGGDSTDGDATAEHF
RRGGENGSYRRGFSWHVYGYS*R*ENKTN*LFG
GLQTVLGWSFSGVS*TVYPVDC*VYSWSA*S*KN
FFSL*LLSNGS*DWSPSTQAGL*IPDKL*HS*V
GKNTALGLNI*TGSENNWGSGLQGCSRSLKSDF
GEDFDNS*YSELCCCTAASGSKRGYSIYLGKKCL
LITSLFCSH*DQETVS*RQTSTLVTWKPSIRLC
GYLQAHSKDKLHLWSL*SSASCK*FGCHL*FME
TGSCYSSFSFERPFAI**GSV*TTDCFVEICIGA
ALFQGYGLQYARFK*AAQAALPCAGGPVGGSGC
LCHGAI*DRGEV*RWGNKPTPVAASLKSAHFLC
AFPVCKFSTYGAFSSSEVSRARTD*RQRSSYVGS
PAIHFWKYSEKCTS*FSPCDEALRLAIPRKRIY
PSS*Y*QTPVNPCLCNDLYLDSSQ*KSSK*QLQ
ATDSNTSFPKTSP*VPAAESKK*KFTDE*L*DCS
IV*CILYKFRMFYITHGSSGRNYLWKWNYEDTS
PWNKLYGFRIYYPLTYEPPGFTDSSCQNEPYSQ
HCNQGDKTCSCKVQCGLGSSPSGNLQSFIGLYGN
RVFGHQRIYQSAFANCFQITCMGDLTHTP*DV*
LPDASYSASLQSSAPESSSYFGCSCTNKPEPAP
SLCREHCSQAYNSIR*LRGTTAVYTLP**SQNSA
LSRI*RTEPSLDIDLG*SNSCNRFFYRL*FNSG
NLV*RHTSDHHEFHSS*LGFTHPELFSRPTTGI
LQTK*CASGKPF*SEKKCGGGV*EVEVNEQRKRH
YYPLLYAGLPSSLSLSSLENALGNRSY*SDWL*
SIRENWSQGLGSPCEDICRFPGI*VFYISRGSA
TQ*MH*NS**HGMEV*HCYTGQINSLPGHA*SRR
K*SPGLLFHNSVAVTQTKRF*KSSK*LCEGKFP
RALVTE*LAHQAHELSQEISREVVF*GPRGTGG
SSCTDPVSLSAHLFWECVSSIPSSI*YSNPQIFR
VASGIQITGDSTGSSRRLI*IS*SSSDLSV*HS
ALL*NAPERPRISQTKTRPCDHWLSEG*STAGL
VSK*HLPEMRYECTRGKSLGSR*HLLLQIDWQTS
RYDGWQISWSLSKL*LEIQ*VSQPSCPCSPCYL
CGAHGLGSFRQRSWECPSKCCPKKSAFSAKREH
YSMDECNWFDHHCPTRAILDCSS*SNCECHQQPQ
LDV*NRVGWLSIPPL*FHCLSSVLL*DEL*LYV
SSCTCCVAPF*HRTTFSHSKVSY*STSSYSEDR
IPVALCIPSCWTIFTKISARENSLYDRDWCGVL*
HAAEC*PV*HPFKLHGSHL*LPLSHEVYVYW*Q
RERASREDYL*LKTSFKTSSSIHHTH*QDGASC
SASTSHEQWVSSTSV*SGARVFTSNSVKPDCTVE
KVEIYPSLRVDSNLLNLNAITL**YSRSSDDIQ
IVLF*FK**IC*HLITPISLSIFLSLRDQVRYM
LFIFITTY*SYVGT*RYL*SNDLSLALSVFPILA
VVFVCIFYY**HI*SSDILF*RHHTCKTVITNI
LYINLLVDFQNNRDLYQEKESRFCCIVGANKAS
YSF*NDLKFYYKFP*FLSRSTGSFITFAMDLNLL
YKISWKPCKKITSENS*T*NHA*MVDSPISFFK
NKLIAFAG*HSHLTDDVILQFL*S*NF*ILYL*
IQYTFYFLDFERHC*FWGNWHCERLEN**VKSAE
*INRVIVS

Sequence NM_015979

GCTGTTGGCTGGGCTCGGGAGGGAACGCTCCAGGTAAAGCGCTGGGGGAGGAAGCGACGCCGAGGAGCTACGGTTTCCTCCAGAGGTCTCCGCCCCTCTG
CCCCTATATTCCCAGAGCTCGAGTCTGATCCGGGCCTTGCCGGGCACCCTGGAAAGGCGGGGGTGATAGTACAGATGGAGACGCAACTGCAGAGCATTTT
CGAAGAGGTGGTGAAAACGGAAGTTATAGAAGAGGCTTTTCCTGGCATGTTTATGGATACTCCTGAAGATGAGAAAACAAAACTAATTAGCTGTTTGGGG
GCCTTCAGACAGTTTTGGGGTGGTCTTTCTCAGGAGTCTCATGAACAGTGTATCCAGTGGATTGTTAAGTTTATTCATGGTCAGCATAGTCCTAAAAGAA
TTTCTTTTCTTTATGACTGCTTAGCAATGGCAGTTGAGACTGGTCTCCTTCCACCCAGGCTGGTTTGTGAATCCCTGATAAACTCTGACACTCTTGAGTG
GGAAAGAACACAGCTTTGGGCCTTAACATTTAAACTGGTTCGGAAAATAATTGGGGGAGTGGATTACAAGGGTGTTCGAGATCTCTTAAAAGTGATTTTG
GAGAAGATTTTGACAATTCCTAATACAGTGAGCTCTGCTGTTGTACAGCAGCTTCTGGCAGCAAGAGAGGTTATAGCATATATCTTGGAAAGAAATGCCT
GCTTATTACCAGCCTATTTTGCAGTCACTGAGATCAGGAAACTGTATCCTGAAGGCAAACTTCCACACTGGTTACTTGGAAACCTAGTATCAGACTTTGT
GGATACCTTCAGGCCCACAGCAAGGATAAACTCCATTTGTGGTCGCTGTAGTCTTCTGCCAGTTGTAAATAATTCGGGTGCCATTTGTAATTCATGGAAA
CTGGATCCTGCTACTCTTCGTTTTCCTTTGAAAGGCCTTTTGCCATATGATAAGGATCTGTTTGAACCACAGACTGCTTTGTTGAGATATGTATTGGAGC
AGCCTTATTCCAGGGATATGGTCTGCAATATGCTAGGTTTAAATAAGCAGACCTTGAACATTGCTCAGCACAAGCAGCGCTGCCCTGTGCTGGAGGACCA
GTTGGTGGATCTGGTTGTTTATGCCATGGAGCGATCTGAGACCGAGGAGAAGTTTGACGATGGGGGAACAAGCCAACTCCTGTGGCAGCATCTCTCAAGT
CAGCTCATTTTCTTTGTGCTTTTCCAGTTTGCAAGTTTTCCACATATGGTGCTTTCTCTTCATCAGAAGTTAGCAGGGCGAGGACTGATTAAAGGCAGAG
ATCATCTTATGTGGGTTCTCCTGCAATTCATTTCTGGAAGTATTCAGAAAAATGCACTAGCTGATTTTCTCCCTGTGATGAAGCTCTTCGACTTGCTATA
CCCAGAAAAAGAATATATCCCAGTTCCTGATATTAACAAACCCCAGTCAACCCATGCCTTTGCAATGACCTGTATTTGGATTCATCTCAATAGAAAAGCT
CAAAATGACAACTCCAAGCTACAGATTCCAATACCTCATTCCCTAAGACTTCACCATGAGTTCCTGCAGCAGAGTCTAAGAAATAAAAGTTTACAGATGA
ATGACTATAAGATTGCTCTATTGTGTAATGCATACTCTACAAATTCAGAATGTTTTACATTACCCATGGGAGCTCTGGTAGAAACTATTTATGGAAATGG
AATTATGAGGATACCTCTCCCTGGAACAAACTGTATGGCTTCAGGATCTATTACCCCCTTACCTATGAACCTCCTGGATTCACTGACAGTTCATGCCAAA
ATGAGCCTTATTCACAGCATTGCAACCAGGGTGATAAAACTTGCTCATGCAAAGTCCAGTGTGGCCTTGGCTCCAGCCCTAGTGGAAACTTACAGTCGTT
TATTGGTCTATATGGAAATAGAGTCTTTGGGCATCAAAGGATTTATCAGTCAGCTTTTGCCAACTGTTTTCAAATCACATGCATGGGGGATCTTACACAC
ACTCCTTGAGATGTTTAGCTACCGGATGCATCATATTCAGCCTCATTACAGAGTTCAGCTCCTGAGTCATCTTCATACTTTGGCTGCAGTTGCACAAACA
AACCAGAACCAGCTCCATCTTTGTGTCGAGAGCACTGCTCTCAGGCTTATAACAGCATTAGGTAGCTCAGAGGTACAACCGCAGTTTACACGCTTCCTTA
GTGATCCCAAAACAGTGCTCTCAGCAGAATCTGAAGAACTGAACCGAGCCTTGATATTGACCTTGGCTAGAGCAACTCATGTAACAGATTTTTTTACAGG
CTCTGATTCAATTCAGGGAACTTGGTGTAAAGACATACTTCAGACCATCATGAGTTTCACTCCTCATAATTGGGCTTCACACACCCTGAGCTGTTTTCCA
GGCCCACTACAGGCATTCTTCAAACAAAATAATGTGCCTCAGGAAAGCCGTTTTAATCTGAAAAAAAATGTGGAGGAGGAGTATAGGAAGTGGAAGTCAA
TGAGCAACGAAAACGACATTATTACCCACTTCTCTATGCAGGGCTCCCCTCCTCTCTTTCTTTGTCTTCTCTGGAAAATGCTCTTGGAAACAGATCATAT
TAATCAGATTGGCTATAGAGTATTAGAGAGAATTGGAGCCAGGGCCTTGGTAGCCCATGTGAGGACATTTGCAGATTTCCTGGTATATGAGTTTTCTACA
TCAGCAGGGGGTCAGCAACTCAATAAATGCATTGAAATTCTTAATGACATGGTATGGAAGTATAACATTGTTACACTGGACAGATTAATTCTCTGCCTGG
CCATGCGTAGTCACGAAGGAAATGAAGCCCAGGTTTGTTATTTCATAATTCAGTTGCTGTTACTCAAACCAAACGATTTTAGAAATCGAGTAAGTGACTT
TGTGAAGGAAAATTCCCCAGAGCACTGGTTACAGAATGACTGGCACACCAAGCACATGAATTATCACAAGAAATATCCAGAGAAGTTGTATTTTGAGGGC
CTCGCGGAACAGGTGGATCCTCCTGTACAGATCCAGTCTCCCTATCTGCCCATCTATTTTGGGAATGTGTGTCTTCGATTCCTTCCAGTATTTGATATAG
TAATCCACAGATTTTTAGAGTTGCTTCCGGTATCCAAATCACTGGAGACTCTACTGGATCATCTAGGAGGCTTATATAAATTTCATGATCGTCCAGTGAC
TTATCTGTATAACACTCTGCACTATTATGAAATGCACCTGAGAGACCGCGCATTTCTCAAACGAAAACTCGTCCATGCGATCATTGGCTCTCTGAAGGAT
AATCGACCGCAGGGCTGGTGTCTAAGTGACACTTACCTGAAATGCGCTATGAATGCACGAGAGGAAAATCCTTGGGTTCCAGATGACACCTACTATTGCA
GATTGATTGGCAGACTAGTCGATACGATGGCTGGCAAATCTCCTGGTCCCTTTCCAAACTGTGACTGGAGATTCAATGAGTTTCCCAACCCAGCTGCCCA
TGCTCTCCATGTTACTTGTGTGGAGCTCATGGCCTTGGCAGTTTCAGGCAAAGAAGTTGGGAATGCCCTTCTAAATGTTGTCCTAAAAAGTCAGCCTTTA
GTGCCAAGAGAGAACATTACAGCATGGATGAATGCAATTGGTTTGATCATCACTGCCCTACCAGAGCCATATTGGATTGTTCTTCATGATCGAATTGTGA
GTGTCATCAGCAGCCCCAGCTTGACGTCTGAAACAGAGTGGGTTGGCTATCCATTCCGCCTCTTTGATTTCACTGCCTGTCATCAGTCCTACTCTGAGAT
GAGTTGTAGCTATACGTTAGCTCTTGCACATGCTGTGTGGCACCATTCTAGCATCGGACAACTTTCTCTCATTCCAAAGTTTCTTACTGAAGTACTTCTT
CCTATAGTGAAGACCGAATTCCAGTTGCTTTATGTATACCATCTTGTTGGACCATTTTTACAAAGATTTCAGCAAGAGAGAACTCGTTGTATGATAGAGA
TTGGTGTGGCGTTTTATGACATGCTGCTGAATGTTGACCAGTGTAGCACCCATTTAAATTACATGGATCCCATCTGTGACTTCCTCTATCACATGAAGTA
TATGTTTACTGGTGACAGCGTGAAAGAGCAAGTAGAGAAGATTATCTGTAACTTAAAACCAGCTTTAAAACTTCGTCTTCGATTCATCACACACATTAGC
AAGATGGAGCCAGCTGCAGTGCCTCCACAAGCCATGAACAGTGGGTCTCCAGCACCTCAGTCTAATCAGTAAAAAGTCAGAGTACACAGCAAGGGTTCCA
AATTAAGAGGAAGCAAAACGCTGCCCAGATCCTGAGAAACCTGGAGAAAATCAACCTTAGTAGTTTCAACTCATACATTGTCTTGTTTGATTTTGTTCTT
TGTTTTTTATCCTCTTTAAGCACAGATGCGTGTTGAAGTCTAAGGTGTTCTGTCATCACACTCTCTTTACTAGAATAAGTGTGCCCAGGCAGAAAAAAAA
GTGTATTCAATGTTGCTGGCATGTGTGGATTCACAGCTCGTGGCTGTAAGGAAATCTGGGGGTTTCATAAATCCATTAAAATCCTTTTAGAGTTGCCAA
  • refseq_CRSP3.F1 (forward primer)
  • refseq_CRSP3.R1 (reverse primer)
  • Single exon skipping

Frame 0

AVGWAREGTLQVKRWGRKRRRGATVSSRGLRPS
APIFPELESDPGLAGHPGKAGVIVQMETQLQSIF
EEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLG
AFRQFWGGLSQESHEQCIQWIVKFIHGQHSPKR
ISFLYDCLAMAVETGLLPPRLVCESLINSDTLEW
ERTQLWALTFKLVRKIIGGVDYKGVRDLLKVIL
EKILTIPNTVSSAVVQQLLAAREVIAYILERNA
CLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFV
DTFRPTARINSICGRCSLLPVVNNSGAICNSWK
LDPATLRFPLKGLLPYDKDLFEPQTALLRYVLE
QPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQ
LVDLVVYAMERSETEEKFDDGGTSQLLWQHLSS
QLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGR
DHLMWVLLQFISGSIQKNALADFLPVMKLFDLLY
PEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKA
QNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQM
NDYKIALLCNAYSTNSECFTLPMGALVETIYGNG
IMRIPLPGTNCMASGSITPLPMNLLDSLTVHAK
MSLIHSIATRVIKLAHAKSSVALAPALVETYSR
LLVYMEIESLGIKGFISQLLPTVFKSHAWGILHT
LLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQT
NQNQLHLCVESTALRLITALGSSEVQPQFTRFL
SDPKTVLSAESEELNRALILTLARATHVTDFFTG
SDSIQGTWCKDILQTIMSFTPHNWASHTLSCFP
GPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKS
MSNENDIITHFSMQGSPPLFLCLLWKMLLETDHI
NQIGYRVLERIGARALVAHVRTFADFLVYEFST
SAGGQQLNKCIEILNDMVWKYNIVTLDRLILCL
AMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDF
VKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEG
LAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDI
VIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVT
YLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKD
NRPQGWCLSDTYLKCAMNAREENPWVPDDTYYC
RLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAH
ALHVTCVELMALAVSGKEVGNALLNVVLKSQPL
VPRENITAWMNAIGLIITALPEPYWIVLHDRIV
SVISSPSLTSETEWVGYPFRLFDFTACHQSYSEM
SCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLL
PIVKTEFQLLYVYHLVGPFLQRFQQERTRCMIE
IGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKY
MFTGDSVKEQVEKIICNLKPALKLRLRFITHIS
KMEPAAVPPQAMNSGSPAPQSNQ*KVRVHSKGS
KLRGSKTLPRS*ETWRKSTLVVSTHTLSCLILFF
VFYPL*AQMRVEV*GVLSSHSLY*NKCAQAEKK
VYSMLLACVDSQLVAVRKSGGFINPLKSF*SCQ

Frame 1

LLAGLGRERSR*SAGGGSDAEELRFPPEVSAPL
PLYSQSSSLIRALPGTLERRG**YRWRRNCRAF
SKRW*KRKL*KRLFLACLWILLKMRKQN*LAVWG
PSDSFGVVFLRSLMNSVSSGLLSLFMVSIVLKE
FLFFMTA*QWQLRLVSFHPGWFVNP**TLTLLS
GKEHSFGP*HLNWFGK*LGEWITRVFEIS*K*FW
RRF*QFLIQ*ALLLYSSFWQQERL*HISWKEMP
AYYQPILQSLRSGNCILKANFHTGYLET*YQTL
WIPSGPQQG*TPFVVAVVFCQL*IIRVPFVIHGN
WILLLFVFL*KAFCHMIRICLNHRLLC*DMYWS
SLIPGIWSAIC*V*ISRP*TLLSTSSAALCWRT
SWWIWLFMPWSDLRPRRSLTMGEQANSCGSISQV
SSFSLCFSSLQVFHIWCFLFIRS*QGED*LKAE
IILCGFSCNSFLEVFRKMH*LIFSL**SSSTCY
TQKKNISQFLILTNPSQPMPLQ*PVFGFISIEKL
KMTTPSYRFQYLIP*DFTMSSCSRV*EIKVYR*
MTIRLLYCVMHTLQIQNVLHYPWELW*KLFMEM
EL*GYLSLEQTVWLQDLLPPYL*TSWIH*QFMPK
*ALFTALQPG**NLLMQSPVWPWLQP*WKLTVV
YWSIWK*SLWASKDLSVSFCQLFSNHMHGGSYT
HSLRCLATGCIIFSLITEFSS*VIFILWLQLHKQ
TRTSSIFVSRALLSGL*QH*VAQRYNRSLHASL
VIPKQCSQQNLKN*TEP*Y*PWLEQLM*QIFLQ
ALIQFRELGVKTYFRPS*VSLLIIGLHTP*AVFQ
AHYRHSSNKIMCLRKAVLI*KKMWRRSIGSGSQ
*ATKTTLLPTSLCRAPLLSFFVFSGKCSWKQII
LIRLAIEY*RELEPGPW*PM*GHLQISWYMSFLH
QQGVSNSINALKFLMTWYGSITLLHWTD*FSAW
PCVVTKEMKPRFVIS*FSCCYSNQTILEIE*VT
L*RKIPQSTGYRMTGTPST*IITRNIQRSCILRA
SRNRWILLYRSSLPICPSILGMCVFDSFQYLI*
*STDF*SCFRYPNHWRLYWII*EAYINFMIVQ*
LICITLCTIMKCT*ETAHFSNENSSMRSLAL*RI
IDRRAGV*VTLT*NAL*MHERKILGFQMTPTIA
D*LAD*SIRWLANLLVPFQTVTGDSMSFPTQLP
MLSMLLVWSSWPWQFQAKKLGMPF*MLS*KVSL*
CQERTLQHG*MQLV*SSLPYQSHIGLFFMIEL*
VSSAAPA*RLKQSGLAIHSASLISLPVISPTLR
*VVAIR*LLHMLCGTILASDNFLSFQSFLLKYFF
L**RPNSSCFMYTILLDHFYKDFSKRELVV**R
LVWRFMTCC*MLTSVAPI*ITWIPSVTSSIT*S
ICLLVTA*KSK*RRLSVT*NQL*NFVFDSSHTLA
RWSQLQCLHKP*TVGLQHLSLISKKSEYTARVP
N*EEAKRCPDPEKPGENQP**FQLIHCLV*FCS
LFFILFKHRCVLKSKVFCHHTLFTRISVPRQKKK
CIQCCWHVWIHSSWL*GNLGVS*IH*NPFRVA

Frame 2

CWLGSGGNAPGKALGEEATPRSYGFLQRSPPLC
PYIPRARV*SGPCRAPWKGGGDSTDGDATAEHF
RRGGENGSYRRGFSWHVYGYS*R*ENKTN*LFG
GLQTVLGWSFSGVS*TVYPVDC*VYSWSA*S*KN
FFSL*LLSNGS*DWSPSTQAGL*IPDKL*HS*V
GKNTALGLNI*TGSENNWGSGLQGCSRSLKSDF
GEDFDNS*YSELCCCTAASGSKRGYSIYLGKKCL
LITSLFCSH*DQETVS*RQTSTLVTWKPSIRLC
GYLQAHSKDKLHLWSL*SSASCK*FGCHL*FME
TGSCYSSFSFERPFAI**GSV*TTDCFVEICIGA
ALFQGYGLQYARFK*ADLEHCSAQAALPCAGGP
VGGSGCLCHGAI*DRGEV*RWGNKPTPVAASLK
SAHFLCAFPVCKFSTYGAFSSSEVSRARTD*RQR
SSYVGSPAIHFWKYSEKCTS*FSPCDEALRLAI
PRKRIYPSS*Y*QTPVNPCLCNDLYLDSSQ*KS
SK*QLQATDSNTSFPKTSP*VPAAESKK*KFTDE
*L*DCSIV*CILYKFRMFYITHGSSGRNYLWKW
NYEDTSPWNKLYGFRIYYPLTYEPPGFTDSSCQ
NEPYSQHCNQGDKTCSCKVQCGLGSSPSGNLQSF
IGLYGNRVFGHQRIYQSAFANCFQITCMGDLTH
TP*DV*LPDASYSASLQSSAPESSSYFGCSCTN
KPEPAPSLCREHCSQAYNSIR*LRGTTAVYTLP*
*SQNSALSRI*RTEPSLDIDLG*SNSCNRFFYR
L*FNSGNLV*RHTSDHHEFHSS*LGFTHPELFS
RPTTGILQTK*CASGKPF*SEKKCGGGV*EVEVN
EQRKRHYYPLLYAGLPSSLSLSSLENALGNRSY
*SDWL*SIRENWSQGLGSPCEDICRFPGI*VFY
ISRGSATQ*MH*NS**HGMEV*HCYTGQINSLPG
HA*SRRK*SPGLLFHNSVAVTQTKRF*KSSK*L
CEGKFPRALVTE*LAHQAHELSQEISREVVF*G
PRGTGGSSCTDPVSLSAHLFWECVSSIPSSI*YS
NPQIFRVASGIQITGDSTGSSRRLI*IS*SSSD
LSV*HSALL*NAPERPRISQTKTRPCDHWLSEG
*STAGLVSK*HLPEMRYECTRGKSLGSR*HLLLQ
IDWQTSRYDGWQISWSLSKL*LEIQ*VSQPSCP
CSPCYLCGAHGLGSFRQRSWECPSKCCPKKSAF
SAKREHYSMDECNWFDHHCPTRAILDCSS*SNCE
CHQQPQLDV*NRVGWLSIPPL*FHCLSSVLL*D
EL*LYVSSCTCCVAPF*HRTTFSHSKVSY*STS
SYSEDRIPVALCIPSCWTIFTKISARENSLYDRD
WCGVL*HAAEC*PV*HPFKLHGSHL*LPLSHEV
YVYW*QRERASREDYL*LKTSFKTSSSIHHTH*
QDGASCSASTSHEQWVSSTSV*SVKSQSTQQGFQ
IKRKQNAAQILRNLEKINLSSFNSYIVLFDFVL
CFLSSLSTDAC*SLRCSVITLSLLE*VCPGRKK
SVFNVAGMCGFTARGCKEIWGFHKSIKILLELP

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