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Sequences

Sequence NM_020351

CCGATCCTCTCCGTGGGAGCCAGCGAGCCTCTCTCCCTGATCTTACGTGCTCAAGAAGCTGTTGTGAAGGCAGAGCAGCATCTGCTGAAGAGACAGAAAC
CAGCCCCAGAGGTGTCACAGGAAGGCACCAGCAAGGACATTGGTCTTTGATTTGATTCAGCAGTCCTGTCAAGTATAAATGTGATGGCTGTGCTGCCTGG
CCCTCTGCAGCTGCTGGGAGTGCTGCTTACCATTTCCCTGAGTTCCATCAGGCTCATTCAGGCTGGTGCCTACTATGGGATCAAGCCGCTGCCACCTCAA
ATTCCTCCTCAGATGCCACCACAAATTCCACAATACCAGCCCCTGGGTCAGCAAGTACCTCACATGCCTTTGGCCAAAGATGGCCTTGCCATGGGCAAGG
AGATGCCCCACTTGCAGTATGGCAAAGAGTATCCACACCTACCCCAATATATGAAGGAAATTCAACCGGCGCCAAGAATGGGCAAGGAAGCCGTACCCAA
GAAAGGCAAAGAAATACCATTAGCCAGTTTACGAGGGGAACAAGGTCCCCGTGGAGAGCCTGGCCCAAGAGGACCACCTGGGCCCCCTGGTTTGCCAGGT
CATGGGATACCTGGAATTAAAGGAAAACCAGGGCCACAGGGATATCCAGGAGTTGGAAAGCCAGGTATGCCTGGAATGCCAGGGAAGCCAGGAGCCATGG
GCATGCCTGGGGCAAAAGGAGAAATTGGACAGAAAGGGGAAATTGGGCCTATGGGGATCCCAGGACCACAAGGACCTCCAGGGCCTCATGGACTTCCTGG
CATTGGGAAGCCAGGTGGGCCAGGGTTACCAGGGCAACCAGGACCAAAGGGTGATCGAGGACCCAAAGGACTACCAGGACCTCAAGGCCTTCGGGGTCCT
AAAGGAGACAAGGGCTTCGGGATGCCAGGTGCGCCAGGTGTAAAGGGGCCTCCAGGGATGCACGGCCCTCCCGGCCCTGTTGGACTGCCAGGAGTGGGCA
AACCAGGAGTGACAGGCTTCCCTGGGCCCCAGGGCCCCCTGGGAAAGCCAGGGGCTCCAGGAGAACCTGGGCCACAAGGCCCTATTGGGGTACCGGGGGT
TCAAGGACCTCCTGGGATACCCGGAATTGGAAAGCCAGGCCAGGATGGGATCCCAGGCCAGCCAGGATTTCCAGGTGGCAAAGGGGAGCAAGGACTGCCA
GGGCTACCAGGACCCCCAGGCCTTCCAGGGATTGGGAAACCAGGCTTCCCAGGACCCAAAGGTGACCGGGGCATGGGAGGTGTTCCTGGGGCTCTTGGAC
CAAGAGGGGAGAAAGGACCAATAGGTGCCCCAGGAATAGGGGGTCCTCCAGGAGAGCCAGGCCTGCCTGGAATCCCAGGTCCTATGGGCCCTCCAGGTGC
TATTGGTTTTCCTGGACCCAAAGGAGAAGGTGGGATTGTAGGGCCACAGGGGCCACCAGGTCCCAAGGGTGAGCCAGGGCTTCAAGGCTTCCCAGGAAAG
CCAGGTTTCCTTGGTGAAGTAGGGCCTCCTGGCATGAGGGGTTTGCCAGGTCCCATAGGGCCCAAGGGGGAAGCTGGGCAAAAAGGTGTACCAGGACTCC
CTGGTGTTCCAGGGCTTCTCGGACCTAAGGGAGAGCCAGGAATCCCAGGGGATCAGGGTTTACAGGGCCCCCCAGGTATCCCAGGGATTGGGGGCCCTAG
TGGCCCCATTGGACCACCTGGGATTCCAGGCCCCAAAGGGGAGCCGGGCCTCCCAGGGCCCCCTGGGTTCCCTGGTATAGGGAAACCCGGAGTGGCAGGA
CTTCATGGCCCCCCAGGGAAGCCTGGTGCCCTTGGTCCTCAAGGCCAGCCTGGCCTTCCAGGACCCCCAGGCCCTCCAGGACCTCCAGGACCCCCAGCTG
TGATGCCCCCTACACCACCACCCCAGGGAGAGTATCTGCCAGATATGGGGCTGGGAATTGATGGCGTGAAACCCCCCCATGCCTACGGGGCTAAGAAAGG
CAAGAATGGAGGGCCAGCCTATGAGATGCCTGCATTTACCGCCGAGCTAACCGCACCTTTCCCACCGGTGGGGGCCCCAGTGAAGTTTAACAAACTGCTG
TATAACGGCAGACAGAACTACAACCCGCAGACAGGCATCTTCACCTGTGAGGTCCCTGGTGTCTACTACTTTGCATACCACGTTCACTGCAAGGGGGGCA
ACGTGTGGGTTGCTCTATTCAAGAACAACGAGCCCGTGATGTACACGTACGACGAGTACAAAAAGGGCTTCCTGGACCAGGCATCTGGGAGTGCAGTGCT
GCTGCTCAGGCCCGGAGACCGGGTGTTCCTCCAGATGCCCTCAGAACAGGCTGCAGGACTGTATGCCGGGCAGTATGTCCACTCCTCCTTTTCAGGATAT
TTATTGTATCCCATGTAAAAACAAAAAAACAAAAAACAAAGAAAAGAAAGAGATTTTATAGAAGAAAATGACACACCAAAAAATCCAAATGAAAAACATA
ATTGCTTCAAAACACTTACACAGTTGGAAAGTTATATGTAAGTGAAAATTTGGACCATTGTGTACAAATAAAAACTAAGATGCATGTTTAATACTC
  • refseq_COL8A1.F1 (forward primer)
  • refseq_COL8A1.R1 (reverse primer)
  • Single exon skipping

Frame 0

PILSVGASEPLSLILRAQEAVVKAEQHLLKRQK
PAPEVSQEGTSKDIGL*FDSAVLSSINVMAVLPG
PLQLLGVLLTISLSSIRLIQAGAYYGIKPLPPQ
IPPQMPPQIPQYQPLGQQVPHMPLAKDGLAMGK
EMPHLQYGKEYPHLPQYMKEIQPAPRMGKEAVPK
KGKEIPLASLRGEQGPRGEPGPRGPPGPPGLPG
HGIPGIKGKPGPQGYPGVGKPGMPGMPGKPGAM
GMPGAKGEIGQKGEIGPMGIPGPQGPPGPHGLPG
IGKPGGPGLPGQPGPKGDRGPKGLPGPQGLRGP
KGDKGFGMPGAPGVKGPPGMHGPPGPVGLPGVG
KPGVTGFPGPQGPLGKPGAPGEPGPQGPIGVPGV
QGPPGIPGIGKPGQDGIPGQPGFPGGKGEQGLP
GLPGPPGLPGIGKPGFPGPKGDRGMGGVPGALG
PRGEKGPIGAPGIGGPPGEPGLPGIPGPMGPPGA
IGFPGPKGEGGIVGPQGPPGPKGEPGLQGFPGK
PGFLGEVGPPGMRGLPGPIGPKGEAGQKGVPGL
PGVPGLLGPKGEPGIPGDQGLQGPPGIPGIGGPS
GPIGPPGIPGPKGEPGLPGPPGFPGIGKPGVAG
LHGPPGKPGALGPQGQPGLPGPPGPPGPPGPPA
VMPPTPPPQGEYLPDMGLGIDGVKPPHAYGAKKG
KNGGPAYEMPAFTAELTAPFPPVGAPVKFNKLL
YNGRQNYNPQTGIFTCEVPGVYYFAYHVHCKGG
NVWVALFKNNEPVMYTYDEYKKGFLDQASGSAVL
LLRPGDRVFLQMPSEQAAGLYAGQYVHSSFSGY
LLYPM*KQKNKKQRKERDFIEENDTPKNPNEKH
NCFKTLTQLESYM*VKIWTIVYK*KLRCMFNT

Frame 1

RSSPWEPASLSP*SYVLKKLL*RQSSIC*RDRN
QPQRCHRKAPARTLVFDLIQQSCQV*M*WLCCL
ALCSCWECCLPFP*VPSGSFRLVPTMGSSRCHLK
FLLRCHHKFHNTSPWVSKYLTCLWPKMALPWAR
RCPTCSMAKSIHTYPNI*RKFNRRQEWARKPYP
RKAKKYH*PVYEGNKVPVESLAQEDHLGPLVCQV
MGYLELKENQGHRDIQELESQVCLECQGSQEPW
ACLGQKEKLDRKGKLGLWGSQDHKDLQGLMDFL
ALGSQVGQGYQGNQDQRVIEDPKDYQDLKAFGVL
KETRASGCQVRQV*RGLQGCTALPALLDCQEWA
NQE*QASLGPRAPWESQGLQENLGHKALLGYRG
FKDLLGYPELESQARMGSQASQDFQVAKGSKDCQ
GYQDPQAFQGLGNQASQDPKVTGAWEVFLGLLD
QEGRKDQ*VPQE*GVLQESQACLESQVLWALQV
LLVFLDPKEKVGL*GHRGHQVPRVSQGFKASQES
QVSLVK*GLLA*GVCQVP*GPRGKLGKKVYQDS
LVFQGFSDLRESQESQGIRVYRAPQVSQGLGAL
VAPLDHLGFQAPKGSRASQGPLGSLV*GNPEWQD
FMAPQGSLVPLVLKASLAFQDPQALQDLQDPQL
*CPLHHHPRESICQIWGWELMA*NPPMPTGLRK
ARMEGQPMRCLHLPPS*PHLSHRWGPQ*SLTNCC
ITADRTTTRRQASSPVRSLVSTTLHTTFTARGA
TCGLLYSRTTSP*CTRTTSTKRASWTRHLGVQC
CCSGPETGCSSRCPQNRLQDCMPGSMSTPPFQDI
YCIPCKNKKTKNKEKKEIL*KKMTHQKIQMKNI
IASKHLHSWKVICK*KFGPLCTNKN*DACLIL

Frame 2

DPLRGSQRASLPDLTCSRSCCEGRAASAEETET
SPRGVTGRHQQGHWSLI*FSSPVKYKCDGCAAW
PSAAAGSAAYHFPEFHQAHSGWCLLWDQAAATS
NSSSDATTNSTIPAPGSASTSHAFGQRWPCHGQG
DAPLAVWQRVSTPTPIYEGNSTGAKNGQGSRTQ
ERQRNTISQFTRGTRSPWRAWPKRTTWAPWFAR
SWDTWN*RKTRATGISRSWKARYAWNAREARSHG
HAWGKRRNWTERGNWAYGDPRTTRTSRASWTSW
HWEARWARVTRATRTKG*SRTQRTTRTSRPSGS
*RRQGLRDARCARCKGASRDARPSRPCWTARSGQ
TRSDRLPWAPGPPGKARGSRRTWATRPYWGTGG
SRTSWDTRNWKARPGWDPRPARISRWQRGARTA
RATRTPRPSRDWETRLPRTQR*PGHGRCSWGSWT
KRGERTNRCPRNRGSSRRARPAWNPRSYGPSRC
YWFSWTQRRRWDCRATGATRSQG*ARASRLPRK
ARFPW*SRASWHEGFARSHRAQGGSWAKRCTRTP
WCSRASRT*GRARNPRGSGFTGPPRYPRDWGP*
WPHWTTWDSRPQRGAGPPRAPWVPWYRETRSGR
TSWPPREAWCPWSSRPAWPSRTPRPSRTSRTPSC
DAPYTTTPGRVSARYGAGN*WRETPPCLRG*ER
QEWRASL*DACIYRRANRTFPTGGGPSEV*QTA
V*RQTELQPADRHLHL*GPWCLLLCIPRSLQGGQ
RVGCSIQEQRARDVHVRRVQKGLPGPGIWECSA
AAQARRPGVPPDALRTGCRTVCRAVCPLLLFRI
FIVSHVKTKKQKTKKRKRFYRRK*HTKKSK*KT*
LLQNTYTVGKLYVSENLDHCVQIKTKMHV*Y

Sequence NM_001850

CCGATCCTCTCCGTGGGAGCCAGCGAGCCTCTCTCCCTGATCTTACGTGCTCAAGGGAGCTCACACGTTCACCAACTCACCCTTGAAGTCATCTCAAGAA
CAAAAGACAACTGAAAGAAGCTGTTGTGAAGGCAGAGCAGCATCTGCTGAAGAGACAGAAACCAGCCCCAGAGGTGTCACAGGAAGGCACCAGCAAGGAC
ATTGGTCTTTGATTTGATTCAGCAGTCCTGTCAAGTATAAATGTGATGGCTGTGCTGCCTGGCCCTCTGCAGCTGCTGGGAGTGCTGCTTACCATTTCCC
TGAGTTCCATCAGGCTCATTCAGGCTGGTGCCTACTATGGGATCAAGCCGCTGCCACCTCAAATTCCTCCTCAGATGCCACCACAAATTCCACAATACCA
GCCCCTGGGTCAGCAAGTACCTCACATGCCTTTGGCCAAAGATGGCCTTGCCATGGGCAAGGAGATGCCCCACTTGCAGTATGGCAAAGAGTATCCACAC
CTACCCCAATATATGAAGGAAATTCAACCGGCGCCAAGAATGGGCAAGGAAGCCGTACCCAAGAAAGGCAAAGAAATACCATTAGCCAGTTTACGAGGGG
AACAAGGTCCCCGTGGAGAGCCTGGCCCAAGAGGACCACCTGGGCCCCCTGGTTTGCCAGGTCATGGGATACCTGGAATTAAAGGAAAACCAGGGCCACA
GGGATATCCAGGAGTTGGAAAGCCAGGTATGCCTGGAATGCCAGGGAAGCCAGGAGCCATGGGCATGCCTGGGGCAAAAGGAGAAATTGGACAGAAAGGG
GAAATTGGGCCTATGGGGATCCCAGGACCACAAGGACCTCCAGGGCCTCATGGACTTCCTGGCATTGGGAAGCCAGGTGGGCCAGGGTTACCAGGGCAAC
CAGGACCAAAGGGTGATCGAGGACCCAAAGGACTACCAGGACCTCAAGGCCTTCGGGGTCCTAAAGGAGACAAGGGCTTCGGGATGCCAGGTGCGCCAGG
TGTAAAGGGGCCTCCAGGGATGCACGGCCCTCCCGGCCCTGTTGGACTGCCAGGAGTGGGCAAACCAGGAGTGACAGGCTTCCCTGGGCCCCAGGGCCCC
CTGGGAAAGCCAGGGGCTCCAGGAGAACCTGGGCCACAAGGCCCTATTGGGGTACCGGGGGTTCAAGGACCTCCTGGGATACCCGGAATTGGAAAGCCAG
GCCAGGATGGGATCCCAGGCCAGCCAGGATTTCCAGGTGGCAAAGGGGAGCAAGGACTGCCAGGGCTACCAGGACCCCCAGGCCTTCCAGGGATTGGGAA
ACCAGGCTTCCCAGGACCCAAAGGTGACCGGGGCATGGGAGGTGTTCCTGGGGCTCTTGGACCAAGAGGGGAGAAAGGACCAATAGGTGCCCCAGGAATA
GGGGGTCCTCCAGGAGAGCCAGGCCTGCCTGGAATCCCAGGTCCTATGGGCCCTCCAGGTGCTATTGGTTTTCCTGGACCCAAAGGAGAAGGTGGGATTG
TAGGGCCACAGGGGCCACCAGGTCCCAAGGGTGAGCCAGGGCTTCAAGGCTTCCCAGGAAAGCCAGGTTTCCTTGGTGAAGTAGGGCCTCCTGGCATGAG
GGGTTTGCCAGGTCCCATAGGGCCCAAGGGGGAAGCTGGGCAAAAAGGTGTACCAGGACTCCCTGGTGTTCCAGGGCTTCTCGGACCTAAGGGAGAGCCA
GGAATCCCAGGGGATCAGGGTTTACAGGGCCCCCCAGGTATCCCAGGGATTGGGGGCCCTAGTGGCCCCATTGGACCACCTGGGATTCCAGGCCCCAAAG
GGGAGCCGGGCCTCCCAGGGCCCCCTGGGTTCCCTGGTATAGGGAAACCCGGAGTGGCAGGACTTCATGGCCCCCCAGGGAAGCCTGGTGCCCTTGGTCC
TCAAGGCCAGCCTGGCCTTCCAGGACCCCCAGGCCCTCCAGGACCTCCAGGACCCCCAGCTGTGATGCCCCCTACACCACCACCCCAGGGAGAGTATCTG
CCAGATATGGGGCTGGGAATTGATGGCGTGAAACCCCCCCATGCCTACGGGGCTAAGAAAGGCAAGAATGGAGGGCCAGCCTATGAGATGCCTGCATTTA
CCGCCGAGCTAACCGCACCTTTCCCACCGGTGGGGGCCCCAGTGAAGTTTAACAAACTGCTGTATAACGGCAGACAGAACTACAACCCGCAGACAGGCAT
CTTCACCTGTGAGGTCCCTGGTGTCTACTACTTTGCATACCACGTTCACTGCAAGGGGGGCAACGTGTGGGTTGCTCTATTCAAGAACAACGAGCCCGTG
ATGTACACGTACGACGAGTACAAAAAGGGCTTCCTGGACCAGGCATCTGGGAGTGCAGTGCTGCTGCTCAGGCCCGGAGACCGGGTGTTCCTCCAGATGC
CCTCAGAACAGGCTGCAGGACTGTATGCCGGGCAGTATGTCCACTCCTCCTTTTCAGGATATTTATTGTATCCCATGTAAAAACAAAAAAACAAAAAACA
AAGAAAAGAAAGAGATTTTATAGAAGAAAATGACACACCAAAAAATCCAAATGAAAAACATAATTGCTTCAAAACACTTACACAGTTGGAAAGTTATATG
TAAGTGAAAATTTGGACCATTGTGTACAAATAAAAACTAAGATGCATGTTTAATACTC
  • refseq_COL8A1.F1 (forward primer)
  • refseq_COL8A1.R1 (reverse primer)
  • Single exon skipping

Frame 0

PILSVGASEPLSLILRAQGSSHVHQLTLEVISR
TKDN*KKLL*RQSSIC*RDRNQPQRCHRKAPART
LVFDLIQQSCQV*M*WLCCLALCSCWECCLPFP
*VPSGSFRLVPTMGSSRCHLKFLLRCHHKFHNT
SPWVSKYLTCLWPKMALPWARRCPTCSMAKSIHT
YPNI*RKFNRRQEWARKPYPRKAKKYH*PVYEG
NKVPVESLAQEDHLGPLVCQVMGYLELKENQGH
RDIQELESQVCLECQGSQEPWACLGQKEKLDRKG
KLGLWGSQDHKDLQGLMDFLALGSQVGQGYQGN
QDQRVIEDPKDYQDLKAFGVLKETRASGCQVRQ
V*RGLQGCTALPALLDCQEWANQE*QASLGPRAP
WESQGLQENLGHKALLGYRGFKDLLGYPELESQ
ARMGSQASQDFQVAKGSKDCQGYQDPQAFQGLG
NQASQDPKVTGAWEVFLGLLDQEGRKDQ*VPQE*
GVLQESQACLESQVLWALQVLLVFLDPKEKVGL
*GHRGHQVPRVSQGFKASQESQVSLVK*GLLA*
GVCQVP*GPRGKLGKKVYQDSLVFQGFSDLRESQ
ESQGIRVYRAPQVSQGLGALVAPLDHLGFQAPK
GSRASQGPLGSLV*GNPEWQDFMAPQGSLVPLV
LKASLAFQDPQALQDLQDPQL*CPLHHHPRESIC
QIWGWELMA*NPPMPTGLRKARMEGQPMRCLHL
PPS*PHLSHRWGPQ*SLTNCCITADRTTTRRQA
SSPVRSLVSTTLHTTFTARGATCGLLYSRTTSP*
CTRTTSTKRASWTRHLGVQCCCSGPETGCSSRC
PQNRLQDCMPGSMSTPPFQDIYCIPCKNKKTKN
KEKKEIL*KKMTHQKIQMKNIIASKHLHSWKVIC
K*KFGPLCTNKN*DACLIL

Frame 1

RSSPWEPASLSP*SYVLKGAHTFTNSPLKSSQE
QKTTERSCCEGRAASAEETETSPRGVTGRHQQG
HWSLI*FSSPVKYKCDGCAAWPSAAAGSAAYHFP
EFHQAHSGWCLLWDQAAATSNSSSDATTNSTIP
APGSASTSHAFGQRWPCHGQGDAPLAVWQRVST
PTPIYEGNSTGAKNGQGSRTQERQRNTISQFTRG
TRSPWRAWPKRTTWAPWFARSWDTWN*RKTRAT
GISRSWKARYAWNAREARSHGHAWGKRRNWTER
GNWAYGDPRTTRTSRASWTSWHWEARWARVTRAT
RTKG*SRTQRTTRTSRPSGS*RRQGLRDARCAR
CKGASRDARPSRPCWTARSGQTRSDRLPWAPGP
PGKARGSRRTWATRPYWGTGGSRTSWDTRNWKAR
PGWDPRPARISRWQRGARTARATRTPRPSRDWE
TRLPRTQR*PGHGRCSWGSWTKRGERTNRCPRN
RGSSRRARPAWNPRSYGPSRCYWFSWTQRRRWDC
RATGATRSQG*ARASRLPRKARFPW*SRASWHE
GFARSHRAQGGSWAKRCTRTPWCSRASRT*GRA
RNPRGSGFTGPPRYPRDWGP*WPHWTTWDSRPQR
GAGPPRAPWVPWYRETRSGRTSWPPREAWCPWS
SRPAWPSRTPRPSRTSRTPSCDAPYTTTPGRVS
ARYGAGN*WRETPPCLRG*ERQEWRASL*DACIY
RRANRTFPTGGGPSEV*QTAV*RQTELQPADRH
LHL*GPWCLLLCIPRSLQGGQRVGCSIQEQRAR
DVHVRRVQKGLPGPGIWECSAAAQARRPGVPPDA
LRTGCRTVCRAVCPLLLFRIFIVSHVKTKKQKT
KKRKRFYRRK*HTKKSK*KT*LLQNTYTVGKLY
VSENLDHCVQIKTKMHV*Y

Frame 2

DPLRGSQRASLPDLTCSRELTRSPTHP*SHLKN
KRQLKEAVVKAEQHLLKRQKPAPEVSQEGTSKD
IGL*FDSAVLSSINVMAVLPGPLQLLGVLLTIS
LSSIRLIQAGAYYGIKPLPPQIPPQMPPQIPQYQ
PLGQQVPHMPLAKDGLAMGKEMPHLQYGKEYPH
LPQYMKEIQPAPRMGKEAVPKKGKEIPLASLRG
EQGPRGEPGPRGPPGPPGLPGHGIPGIKGKPGPQ
GYPGVGKPGMPGMPGKPGAMGMPGAKGEIGQKG
EIGPMGIPGPQGPPGPHGLPGIGKPGGPGLPGQ
PGPKGDRGPKGLPGPQGLRGPKGDKGFGMPGAPG
VKGPPGMHGPPGPVGLPGVGKPGVTGFPGPQGP
LGKPGAPGEPGPQGPIGVPGVQGPPGIPGIGKP
GQDGIPGQPGFPGGKGEQGLPGLPGPPGLPGIGK
PGFPGPKGDRGMGGVPGALGPRGEKGPIGAPGI
GGPPGEPGLPGIPGPMGPPGAIGFPGPKGEGGI
VGPQGPPGPKGEPGLQGFPGKPGFLGEVGPPGMR
GLPGPIGPKGEAGQKGVPGLPGVPGLLGPKGEP
GIPGDQGLQGPPGIPGIGGPSGPIGPPGIPGPK
GEPGLPGPPGFPGIGKPGVAGLHGPPGKPGALGP
QGQPGLPGPPGPPGPPGPPAVMPPTPPPQGEYL
PDMGLGIDGVKPPHAYGAKKGKNGGPAYEMPAF
TAELTAPFPPVGAPVKFNKLLYNGRQNYNPQTGI
FTCEVPGVYYFAYHVHCKGGNVWVALFKNNEPV
MYTYDEYKKGFLDQASGSAVLLLRPGDRVFLQM
PSEQAAGLYAGQYVHSSFSGYLLYPM*KQKNKKQ
RKERDFIEENDTPKNPNEKHNCFKTLTQLESYM
*VKIWTIVYK*KLRCMFNT

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