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Sequences

Sequence NM_001699

GAGTGGAGTTCTGGAGGAATGTTTACCAGACACAGAGCCCAGAGGGACAGCGCCCAGAGCCCGGATAGAGAGACACGGCCTCACTGGCTCAGGACAGGGG GCACAGCCACCAGGGTCCCCTTCCCCCTCCTCAGCTCCCTCCCTGGCCCCTTTAAGAAAGAGCTGATCCTCTCCTCTCTTGAGTTAACCCCTGATTGTCC AGGTGGCCCCTGGGCTCTGGCCCTGGTGGGCGGAGGCAAAGGGGGAGCCAGGGGCGGAGAAAGGGTTGCCCAAGTCTGGGAGTGAGGGAAGGAGGCAGGG GTGCTGAGAAGGCGGCTGCTGGGCAGAGCCGGTGGCAAGGGCCTCCCCTGCCGCTGTGCCAGGCAGGCAGTGCCAAATCCGGGGAGCCTGGAGCTGGGGG GAGGGCCGGGGACAGCCCGGCCCTGCCCCCTCCCCCGCTGGGAGCCCAACAACTTCTGAGGAAAGTTTGGCACCCATGGCGTGGCGGTGCCCCAGGATGG GCAGGGTCCCGCTGGCCTGGTGCTTGGCGCTGTGCGGCTGGGCGTGCATGGCCCCCAGGGGCACGCAGGCTGAAGAAAGTCCCTTCGTGGGCAACCCAGG GAATATCACAGGTGCCCGGGGACTCACGGGCACCCTTCGGTGTCAGCTCCAGGTTCAGGGAGAGCCCCCCGAGGTACATTGGCTTCGGGATGGACAGATC CTGGAGCTCGCGGACAGCACCCAGACCCAGGTGCCCCTGGGTGAGGATGAACAGGATGACTGGATAGTGGTCAGCCAGCTCAGAATCACCTCCCTGCAGC TTTCCGACACGGGACAGTACCAGTGTTTGGTGTTTCTGGGACATCAGACCTTCGTGTCCCAGCCTGGCTATGTTGGGCTGGAGGGCTTGCCTTACTTCCT GGAGGAGCCCGAAGACAGGACTGTGGCCGCCAACACCCCCTTCAACCTGAGCTGCCAAGCTCAGGGACCCCCAGAGCCCGTGGACCTACTCTGGCTCCAG GATGCTGTCCCCCTGGCCACGGCTCCAGGTCACGGCCCCCAGCGCAGCCTGCATGTTCCAGGGCTGAACAAGACATCCTCTTTCTCCTGCGAAGCCCATA ACGCCAAGGGGGTCACCACATCCCGCACAGCCACCATCACAGTGCTCCCCCAGCAGCCCCGTAACCTCCACCTGGTCTCCCGCCAACCCACGGAGCTGGA GGTGGCTTGGACTCCAGGCCTGAGCGGCATCTACCCCCTGACCCACTGCACCCTGCAGGCTGTGCTGTCAAACGATGGGATGGGCATCCAGGCGGGAGAA CCAGACCCCCCAGAGGAGCCCCTCACCTCGCAAGCATCCGTGCCCCCCCATCAGCTTCGGCTAGGCAGCCTCCATCCTCACACCCCTTATCACATCCGCG TGGCATGCACCAGCAGCCAGGGCCCCTCATCCTGGACCCACTGGCTTCCTGTGGAGACGCCGGAGGGAGTGCCCCTGGGCCCCCCTGAGAACATTAGTGC TACGCGGAATGGGAGCCAGGCCTTCGTGCATTGGCAAGAGCCCCGGGCGCCCCTGCAGGGTACCCTGTTAGGGTACCGGCTGGCGTATCAAGGCCAGGAC ACCCCAGAGGTGCTAATGGACATAGGGCTAAGGCAAGAGGTGACCCTGGAGCTGCAGGGGGACGGGTCTGTGTCCAATCTGACAGTGTGTGTGGCAGCCT ACACTGCTGCTGGGGATGGACCCTGGAGCCTCCCAGTACCCCTGGAGGCCTGGCGCCCAGTGAAGGAACCTTCAACTCCTGCCTTCTCGTGGCCCTGGTG GTATGTACTGCTAGGAGCAGTCGTGGCCGCTGCCTGTGTCCTCATCTTGGCTCTCTTCCTTGTCCACCGGCGAAAGAAGGAGACCCGTTATGGAGAAGTG TTTGAACCAACAGTGGAAAGAGGTGAACTGGTAGTCAGGTACCGCGTGCGCAAGTCCTACAGTCGTCGGACCACTGAAGCTACCTTGAACAGCCTGGGCA TCAGTGAAGAGCTGAAGGAGAAGCTGCGGGATGTGATGGTGGACCGGCACAAGGTGGCCCTGGGGAAGACTCTGGGAGAGGGAGAGTTTGGAGCTGTGAT GGAAGGCCAGCTCAACCAGGACGACTCCATCCTCAAGGTGGCTGTGAAGACGATGAAGATTGCCATCTGCACGAGGTCAGAGCTGGAGGATTTCCTGAGT GAAGCGGTCTGCATGAAGGAATTTGACCATCCCAACGTCATGAGGCTCATCGGTGTCTGTTTCCAGGGTTCTGAACGAGAGAGCTTCCCAGCACCTGTGG TCATCTTACCTTTCATGAAACATGGAGACCTACACAGCTTCCTCCTCTATTCCCGGCTCGGGGACCAGCCAGTGTACCTGCCCACTCAGATGCTAGTGAA GTTCATGGCAGACATCGCCAGTGGCATGGAGTATCTGAGTACCAAGAGATTCATACACCGGGACCTGGCGGCCAGGAACTGCATGCTGAATGAGAACATG TCCGTGTGTGTGGCGGACTTCGGGCTCTCCAAGAAGATCTACAATGGGGACTACTACCGCCAGGGACGTATCGCCAAGATGCCAGTCAAGTGGATTGCCA TTGAGAGTCTAGCTGACCGTGTCTACACCAGCAAGAGCGATGTGTGGTCCTTCGGGGTGACAATGTGGGAGATTGCCACAAGAGGCCAAACCCCATATCC GGGCGTGGAGAACAGCGAGATTTATGACTATCTGCGCCAGGGAAATCGCCTGAAGCAGCCTGCGGACTGTCTGGATGGACTGTATGCCTTGATGTCGCGG TGCTGGGAGCTAAATCCCCAGGACCGGCCAAGTTTTACAGAGCTGCGGGAAGATTTGGAGAACACACTGAAGGCCTTGCCTCCTGCCCAGGAGCCTGACG AAATCCTCTATGTCAACATGGATGAGGGTGGAGGTTATCCTGAACCCCCTGGAGCTGCAGGAGGAGCTGACCCCCCAACCCAGCCAGACCCTAAGGATTC CTGTAGCTGCCTCACTGCGGCTGAGGTCCATCCTGCTGGACGCTATGTCCTCTGCCCTTCCACAACCCCTAGCCCCGCTCAGCCTGCTGATAGGGGCTCC CCAGCAGCCCCAGGGCAGGAGGATGGTGCCTGAGACAACCCTCCACCTGGTACTCCCTCTCAGGATCCAAGCTAAGCACTGCCACTGGGGAAAACTCCAC CTTCCCACTTTCCCACCCCACGCCTTATCCCCACTTGCAGCCCTGTCTTCCTACCTATCCCACCTCCATCCCAGACAGGTCCCTCCCCTTCTCTGTGCAG TAGCATCACCTTGAAAGCAGTAGCATCACCATCTGTAAAAGGAAGGGGTTGGATTGCAATATCTGAAGCCCTCCCAGGTGTTAACATTCCAAGACTCTAG AGTCCAAGGTTTAAAGAGTCTAGATTCAAAGGTTCTAGGTTTCAAAGATGCTGTGAGTCTTTGGTTCTAAGGACCTGAAATTCCAAAGTCTCTAATTCTA TTAAAGTGCTAAGGTTCTAAGGCCTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGATAGAGTCTCACTGTGTCACCCAGGCTGGAGTGCAGTGG TGCAATCTCGCCTCACTGCAACCTTCACCTACCGAGTTCAAGTGATTTTCCTGCCTTGGCCTCCCAAGTAGCTGGGATTACAGGTGTGTGCCACCACACC CGGCTAATTTTTATATTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTAAAACTCCTGACCTCAAGTGATCTGCCCACCTCAGCCTCCC AAAGTGCTGAGATTACAGGCATGAGCCACTGCACTCAACCTTAAGACCTACTGTTCTAAAGCTCTGACATTATGTGGTTTTAGATTTTCTGGTTCTAACA TTTTTGATAAAGCCTCAAGGTTTTAGGTTCTAAAGTTCTAAGATTCTGATTTTAGGAGCTAAGGCTCTATGAGTCTAGATGTTTATTCTTCTAGAGTTCA GAGTCCTTAAAATGTAAGATTATAGATTCTAAAGATTCTATAGTTCTAGACATGGAGGTTCTAAGGCCTAGGATTCTAAAATGTGATGTTCTAAGGCTCT GAGAGTCTAGATTCTCTGGCTGTAAGGCTCTAGATCATAAGGCTTCAAAATGTTATCTTCTCAAGTTCTAAGATTCTAATGATGATCAATTATAGTTTCT GAGGCTTTATGATAATAGATTCTCTTGTATAAGATCCTAGATCCTAAGGGTCGAAAGCTCTAGAATCTGCAATTCAAAAGTTCCAAGAGTCTAAAGATGG AGTTTCTAAGGTCCGGTGTTCTAAGATGTGATATTCTAAGACTTACTCTAAGATCTTAGATTCTCTGTGTCTAAGATTCTAGATCAGATGCTCCAAGATT CTAGATGATTAAATAAGATTCTAACGGTCTGTTCTGTTTCAAGGCACTCTAGATTCCATTGGTCCAAGATTCCGGATCCTAAGCATCTAAGTTATAAGAC TCTCACACTCAGTTGTGACTAACTAGACACCAAAGTTCTAATAATTTCTAATGTTGGACACCTTTAGGTTCTTTGCTGCATTCTGCCTCTCTAGGACCAT GGTTAAGAGTCCAAGAATCCACATTTCTAAAATCTTATAGTTCTAGGCACTGTAGTTCTAAGACTCAAATGTTCTAAGTTTCTAAGATTCTAAAGGTCCA CAGGTCTAGACTATTAGGTGCAATTTCAAGGTTCTAACCCTATACTGTAGTATTCTTTGGGGTGCCCCTCTCCTTCTTAGCTATCATTGCTTCCTCCTCC CCAACTGTGGGGGTGTGCCCCCTTCAAGCCTGTGCAATGCATTAGGGATGCCTCCTTTCCCGCAGGGGATGGACGATCTCCCACCTTTCGGGCCATGTTG CCCCCGTGAGCCAATCCCTCACCTTCTGAGTACAGAGTGTGGACTCTGGTGCCTCCAGAGGGGCTCAGGTCACATAAAACTTTGTATATCAACGA

refseq_AXL.F1 (forward primer)
refseq_AXL.R1 (reverse primer)
Single exon skipping

Frame 0

EWSSGGMFTRHRAQRDSAQSPDRETRPHWLRTG GTATRVPFPLLSSLPGPFKKELILSSLELTPDCP GGPWALALVGGGKGGARGGERVAQVWE*GKEAG VLRRRLLGRAGGKGLPCRCARQAVPNPGSLELG GGPGTARPCPLPRWEPNNF*GKFGTHGVAVPQDG QGPAGLVLGAVRLGVHGPQGHAG*RKSLRGQPR EYHRCPGTHGHPSVSAPGSGRAPRGTLASGWTD PGARGQHPDPGAPG*G*TG*LDSGQPAQNHLPAA FRHGTVPVFGVSGTSDLRVPAWLCWAGGLALLP GGARRQDCGRQHPLQPELPSSGTPRARGPTLAP GCCPPGHGSRSRPPAQPACSRAEQDILFLLRSP* RQGGHHIPHSHHHSAPPAAP*PPPGLPPTHGAG GGLDSRPERHLPPDPLHPAGCAVKRWDGHPGGR TRPPRGAPHLASIRAPPSASARQPPSSHPLSHPR GMHQQPGPLILDPLASCGDAGGSAPGPP*EH*C YAEWEPGLRALARAPGAPAGYPVRVPAGVSRPG HPRGANGHRAKARGDPGAAGGRVCVQSDSVCGSL HCCWGWTLEPPSTPGGLAPSEGTFNSCLLVALV VCTARSSRGRCLCPHLGSLPCPPAKEGDPLWRS V*TNSGKR*TGSQVPRAQVLQSSDH*SYLEQPGH Q*RAEGEAAGCDGGPAQGGPGEDSGRGRVWSCD GRPAQPGRLHPQGGCEDDEDCHLHEVRAGGFPE *SGLHEGI*PSQRHEAHRCLFPGF*TRELPSTCG HLTFHETWRPTQLPPLFPARGPASVPAHSDASE VHGRHRQWHGVSEYQEIHTPGPGGQELHAE*EH VRVCGGLRALQEDLQWGLLPPGTYRQDASQVDCH *ESS*PCLHQQERCVVLRGDNVGDCHKRPNPIS GRGEQRDL*LSAPGKSPEAACGLSGWTVCLDVA VLGAKSPGPAKFYRAAGRFGEHTEGLASCPGA*R NPLCQHG*GWRLS*TPWSCRRS*PPNPARP*GF L*LPHCG*GPSCWTLCPLPFHNP*PRSAC**GL PSSPRAGGWCLRQPSTWYSLSGSKLSTATGENST FPLSHPTPYPHLQPCLPTYPTSIPDRSLPFSVQ *HHLESSSITICKRKGLDCNI*SPPRC*HSKTL ESKV*RV*IQRF*VSKML*VFGSKDLKFQSL*FY *SAKVLRPTFFFFFFFFFFAIESHCVTQAGVQW CNLASLQPSPTEFK*FSCLGLPSSWDYRCVPPH PANFYIFSRDRVSPCWPGWSKTPDLK*SAHLSLP KC*DYRHEPLHSTLRPTVLKL*HYVVLDFLVLT FLIKPQGFRF*SSKILILGAKAL*V*MFILLEF RVLKM*DYRF*RFYSSRHGGSKA*DSKM*CSKAL RV*ILWL*GSRS*GFKMLSSQVLRF***SIIVS EAL***ILLYKILDPKGRKL*NLQFKSSKSLKM EFLRSGVLRCDILRLTLRS*ILCV*DSRSDAPRF *MIK*DSNGLFCFKAL*IPLVQDSGS*ASKL*D SHTQL*LTRHQSSNNF*CWTPLGSLLHSASLGP WLRVQESTFLKSYSSRHCSSKTQMF*VSKILKVH RSRLLGAISRF*PYTVVFFGVPLSFLAIIASSS PTVGVCPLQACAMH*GCLLSRRGWTISHLSGHV APVSQSLTF*VQSVDSGASRGAQVT*NFVYQR

Frame 1

SGVLEECLPDTEPRGTAPRARIERHGLTGSGQG AQPPGSPSPSSAPSLAPLRKS*SSPLLS*PLIV QVAPGLWPWWAEAKGEPGAEKGLPKSGSEGRRQG C*EGGCWAEPVARASPAAVPGRQCQIRGAWSWG EGRGQPGPAPSPAGSPTTSEESLAPMAWRCPRM GRVPLAWCLALCGWACMAPRGTQAEESPFVGNPG NITGARGLTGTLRCQLQVQGEPPEVHWLRDGQI LELADSTQTQVPLGEDEQDDWIVVSQLRITSLQ LSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFL EEPEDRTVAANTPFNLSCQAQGPPEPVDLLWLQ DAVPLATAPGHGPQRSLHVPGLNKTSSFSCEAH NAKGVTTSRTATITVLPQQPRNLHLVSRQPTELE VAWTPGLSGIYPLTHCTLQAVLSNDGMGIQAGE PDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIR VACTSSQGPSSWTHWLPVETPEGVPLGPPENISA TRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQD TPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAA YTAAGDGPWSLPVPLEAWRPVKEPSTPAFSWPWW YVLLGAVVAAACVLILALFLVHRRKKETRYGEV FEPTVERGELVVRYRVRKSYSRRTTEATLNSLG ISEELKEKLRDVMVDRHKVALGKTLGEGEFGAVM EGQLNQDDSILKVAVKTMKIAICTRSELEDFLS EAVCMKEFDHPNVMRLIGVCFQGSERESFPAPV VILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVK FMADIASGMEYLSTKRFIHRDLAARNCMLNENM SVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIA IESLADRVYTSKSDVWSFGVTMWEIATRGQTPYP GVENSEIYDYLRQGNRLKQPADCLDGLYALMSR CWELNPQDRPSFTELREDLENTLKALPPAQEPD EILYVNMDEGGGYPEPPGAAGGADPPTQPDPKDS CSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGS PAAPGQEDGA*DNPPPGTPSQDPS*ALPLGKTP PSHFPTPRLIPTCSPVFLPIPPPSQTGPSPSLCS SITLKAVASPSVKGRGWIAISEALPGVNIPRL* SPRFKESRFKGSRFQRCCESLVLRT*NSKVSNS IKVLRF*GLLFFFFFFFFFLR*SLTVSPRLECSG AISPHCNLHLPSSSDFPALASQVAGITGVCHHT RLIFIFLVETGFHHVGQAGLKLLTSSDLPTSAS QSAEITGMSHCTQP*DLLF*SSDIMWF*IFWF*H F**SLKVLGSKVLRF*F*ELRLYESRCLFF*SS ESLKCKIIDSKDSIVLDMEVLRPRILKCDVLRL *ESRFSGCKALDHKASKCYLLKF*DSNDDQL*FL RLYDNRFSCIRS*ILRVESSRICNSKVPRV*RW SF*GPVF*DVIF*DLL*DLRFSVSKILDQMLQD SR*LNKILTVCSVSRHSRFHWSKIPDPKHLSYKT LTLSCD*LDTKVLIISNVGHL*VLCCILPL*DH G*ESKNPHF*NLIVLGTVVLRLKCSKFLRF*RS TGLDY*VQFQGSNPIL*YSLGCPSPS*LSLLPPP QLWGCAPFKPVQCIRDASFPAGDGRSPTFRAML PP*ANPSPSEYRVWTLVPPEGLRSHKTLYIN

Frame 2

VEFWRNVYQTQSPEGQRPEPG*RDTASLAQDRG HSHQGPLPPPQLPPWPL*ERADPLLS*VNP*LS RWPLGSGPGGRRQRGSQGRRKGCPSLGVREGGR GAEKAAAGQSRWQGPPLPLCQAGSAKSGEPGAGG RAGDSPALPPPPLGAQQLLRKVWHPWRGGAPGW AGSRWPGAWRCAAGRAWPPGARRLKKVPSWATQ GISQVPGDSRAPFGVSSRFRESPPRYIGFGMDRS WSSRTAPRPRCPWVRMNRMTG*WSASSESPPCS FPTRDSTSVWCFWDIRPSCPSLAMLGWRACLTS WRSPKTGLWPPTPPST*AAKLRDPQSPWTYSGSR MLSPWPRLQVTAPSAACMFQG*TRHPLSPAKPI TPRGSPHPAQPPSQCSPSSPVTSTWSPANPRSW RWLGLQA*AASTP*PTAPCRLCCQTMGWASRREN QTPQRSPSPRKHPCPPISFG*AASILTPLITSA WHAPAARAPHPGPTGFLWRRRRECPWAPLRTLV LRGMGARPSCIGKSPGRPCRVPC*GTGWRIKART PQRC*WT*G*GKR*PWSCRGTGLCPI*QCVWQP TLLLGMDPGASQYPWRPGAQ*RNLQLLPSRGPG GMYC*EQSWPLPVSSSWLSSLSTGERRRPVMEKC LNQQWKEVNW*SGTACASPTVVGPLKLP*TAWA SVKS*RRSCGM*WWTGTRWPWGRLWERESLEL* WKASSTRTTPSSRWL*RR*RLPSARGQSWRIS*V KRSA*RNLTIPTS*GSSVSVSRVLNERASQHLW SSYLS*NMETYTASSSIPGSGTSQCTCPLRC** SSWQTSPVAWSI*VPRDSYTGTWRPGTAC*MRTC PCVWRTSGSPRRSTMGTTTARDVSPRCQSSGLP LRV*LTVSTPARAMCGPSG*QCGRLPQEAKPHI RAWRTARFMTICAREIA*SSLRTVWMDCMP*CRG AGS*IPRTGQVLQSCGKIWRTH*RPCLLPRSLT KSSMSTWMRVEVILNPLELQEELTPQPSQTLRI PVAASLRLRSILLDAMSSALPQPLAPLSLLIGAP QQPQGRRMVPETTLHLVLPLRIQAKHCHWGKLH LPTFPPHALSPLAALSSYLSHLHPRQVPPLLCA VASP*KQ*HHHL*KEGVGLQYLKPSQVLTFQDSR VQGLKSLDSKVLGFKDAVSLWF*GPEIPKSLIL LKC*GSKAYFFFFFFFFFFCDRVSLCHPGWSAV VQSRLTATFTYRVQVIFLPWPPK*LGLQVCATTP G*FLYF**RQGFTMLARLV*NS*PQVICPPQPP KVLRLQA*ATALNLKTYCSKALTLCGFRFSGSN IFDKASRF*VLKF*DSDFRS*GSMSLDVYSSRVQ SP*NVRL*ILKIL*F*TWRF*GLGF*NVMF*GS ESLDSLAVRL*IIRLQNVIFSSSKILMMINYSF *GFMIIDSLV*DPRS*GSKALESAIQKFQESKDG VSKVRCSKM*YSKTYSKILDSLCLRF*IRCSKI LDD*IRF*RSVLFQGTLDSIGPRFRILSI*VIR LSHSVVTN*TPKF**FLMLDTFRFFAAFCLSRTM VKSPRIHISKIL*F*AL*F*DSNVLSF*DSKGP QV*TIRCNFKVLTLYCSILWGAPLLLSYHCFLL PNCGGVPPSSLCNALGMPPFPQGMDDLPPFGPCC PREPIPHLLSTECGLWCLQRGSGHIKLCIST

Sequence NM_021913

GAGTGGAGTTCTGGAGGAATGTTTACCAGACACAGAGCCCAGAGGGACAGCGCCCAGAGCCCGGATAGAGAGACACGGCCTCACTGGCTCAGGACAGGGG GCACAGCCACCAGGGTCCCCTTCCCCCTCCTCAGCTCCCTCCCTGGCCCCTTTAAGAAAGAGCTGATCCTCTCCTCTCTTGAGTTAACCCCTGATTGTCC AGGTGGCCCCTGGGCTCTGGCCCTGGTGGGCGGAGGCAAAGGGGGAGCCAGGGGCGGAGAAAGGGTTGCCCAAGTCTGGGAGTGAGGGAAGGAGGCAGGG GTGCTGAGAAGGCGGCTGCTGGGCAGAGCCGGTGGCAAGGGCCTCCCCTGCCGCTGTGCCAGGCAGGCAGTGCCAAATCCGGGGAGCCTGGAGCTGGGGG GAGGGCCGGGGACAGCCCGGCCCTGCCCCCTCCCCCGCTGGGAGCCCAACAACTTCTGAGGAAAGTTTGGCACCCATGGCGTGGCGGTGCCCCAGGATGG GCAGGGTCCCGCTGGCCTGGTGCTTGGCGCTGTGCGGCTGGGCGTGCATGGCCCCCAGGGGCACGCAGGCTGAAGAAAGTCCCTTCGTGGGCAACCCAGG GAATATCACAGGTGCCCGGGGACTCACGGGCACCCTTCGGTGTCAGCTCCAGGTTCAGGGAGAGCCCCCCGAGGTACATTGGCTTCGGGATGGACAGATC CTGGAGCTCGCGGACAGCACCCAGACCCAGGTGCCCCTGGGTGAGGATGAACAGGATGACTGGATAGTGGTCAGCCAGCTCAGAATCACCTCCCTGCAGC TTTCCGACACGGGACAGTACCAGTGTTTGGTGTTTCTGGGACATCAGACCTTCGTGTCCCAGCCTGGCTATGTTGGGCTGGAGGGCTTGCCTTACTTCCT GGAGGAGCCCGAAGACAGGACTGTGGCCGCCAACACCCCCTTCAACCTGAGCTGCCAAGCTCAGGGACCCCCAGAGCCCGTGGACCTACTCTGGCTCCAG GATGCTGTCCCCCTGGCCACGGCTCCAGGTCACGGCCCCCAGCGCAGCCTGCATGTTCCAGGGCTGAACAAGACATCCTCTTTCTCCTGCGAAGCCCATA ACGCCAAGGGGGTCACCACATCCCGCACAGCCACCATCACAGTGCTCCCCCAGCAGCCCCGTAACCTCCACCTGGTCTCCCGCCAACCCACGGAGCTGGA GGTGGCTTGGACTCCAGGCCTGAGCGGCATCTACCCCCTGACCCACTGCACCCTGCAGGCTGTGCTGTCAAACGATGGGATGGGCATCCAGGCGGGAGAA CCAGACCCCCCAGAGGAGCCCCTCACCTCGCAAGCATCCGTGCCCCCCCATCAGCTTCGGCTAGGCAGCCTCCATCCTCACACCCCTTATCACATCCGCG TGGCATGCACCAGCAGCCAGGGCCCCTCATCCTGGACCCACTGGCTTCCTGTGGAGACGCCGGAGGGAGTGCCCCTGGGCCCCCCTGAGAACATTAGTGC TACGCGGAATGGGAGCCAGGCCTTCGTGCATTGGCAAGAGCCCCGGGCGCCCCTGCAGGGTACCCTGTTAGGGTACCGGCTGGCGTATCAAGGCCAGGAC ACCCCAGAGGTGCTAATGGACATAGGGCTAAGGCAAGAGGTGACCCTGGAGCTGCAGGGGGACGGGTCTGTGTCCAATCTGACAGTGTGTGTGGCAGCCT ACACTGCTGCTGGGGATGGACCCTGGAGCCTCCCAGTACCCCTGGAGGCCTGGCGCCCAGGGCAAGCACAGCCAGTCCACCAGCTGGTGAAGGAACCTTC AACTCCTGCCTTCTCGTGGCCCTGGTGGTATGTACTGCTAGGAGCAGTCGTGGCCGCTGCCTGTGTCCTCATCTTGGCTCTCTTCCTTGTCCACCGGCGA AAGAAGGAGACCCGTTATGGAGAAGTGTTTGAACCAACAGTGGAAAGAGGTGAACTGGTAGTCAGGTACCGCGTGCGCAAGTCCTACAGTCGTCGGACCA CTGAAGCTACCTTGAACAGCCTGGGCATCAGTGAAGAGCTGAAGGAGAAGCTGCGGGATGTGATGGTGGACCGGCACAAGGTGGCCCTGGGGAAGACTCT GGGAGAGGGAGAGTTTGGAGCTGTGATGGAAGGCCAGCTCAACCAGGACGACTCCATCCTCAAGGTGGCTGTGAAGACGATGAAGATTGCCATCTGCACG AGGTCAGAGCTGGAGGATTTCCTGAGTGAAGCGGTCTGCATGAAGGAATTTGACCATCCCAACGTCATGAGGCTCATCGGTGTCTGTTTCCAGGGTTCTG AACGAGAGAGCTTCCCAGCACCTGTGGTCATCTTACCTTTCATGAAACATGGAGACCTACACAGCTTCCTCCTCTATTCCCGGCTCGGGGACCAGCCAGT GTACCTGCCCACTCAGATGCTAGTGAAGTTCATGGCAGACATCGCCAGTGGCATGGAGTATCTGAGTACCAAGAGATTCATACACCGGGACCTGGCGGCC AGGAACTGCATGCTGAATGAGAACATGTCCGTGTGTGTGGCGGACTTCGGGCTCTCCAAGAAGATCTACAATGGGGACTACTACCGCCAGGGACGTATCG CCAAGATGCCAGTCAAGTGGATTGCCATTGAGAGTCTAGCTGACCGTGTCTACACCAGCAAGAGCGATGTGTGGTCCTTCGGGGTGACAATGTGGGAGAT TGCCACAAGAGGCCAAACCCCATATCCGGGCGTGGAGAACAGCGAGATTTATGACTATCTGCGCCAGGGAAATCGCCTGAAGCAGCCTGCGGACTGTCTG GATGGACTGTATGCCTTGATGTCGCGGTGCTGGGAGCTAAATCCCCAGGACCGGCCAAGTTTTACAGAGCTGCGGGAAGATTTGGAGAACACACTGAAGG CCTTGCCTCCTGCCCAGGAGCCTGACGAAATCCTCTATGTCAACATGGATGAGGGTGGAGGTTATCCTGAACCCCCTGGAGCTGCAGGAGGAGCTGACCC CCCAACCCAGCCAGACCCTAAGGATTCCTGTAGCTGCCTCACTGCGGCTGAGGTCCATCCTGCTGGACGCTATGTCCTCTGCCCTTCCACAACCCCTAGC CCCGCTCAGCCTGCTGATAGGGGCTCCCCAGCAGCCCCAGGGCAGGAGGATGGTGCCTGAGACAACCCTCCACCTGGTACTCCCTCTCAGGATCCAAGCT AAGCACTGCCACTGGGGAAAACTCCACCTTCCCACTTTCCCACCCCACGCCTTATCCCCACTTGCAGCCCTGTCTTCCTACCTATCCCACCTCCATCCCA GACAGGTCCCTCCCCTTCTCTGTGCAGTAGCATCACCTTGAAAGCAGTAGCATCACCATCTGTAAAAGGAAGGGGTTGGATTGCAATATCTGAAGCCCTC CCAGGTGTTAACATTCCAAGACTCTAGAGTCCAAGGTTTAAAGAGTCTAGATTCAAAGGTTCTAGGTTTCAAAGATGCTGTGAGTCTTTGGTTCTAAGGA CCTGAAATTCCAAAGTCTCTAATTCTATTAAAGTGCTAAGGTTCTAAGGCCTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGATAGAGTCTCAC TGTGTCACCCAGGCTGGAGTGCAGTGGTGCAATCTCGCCTCACTGCAACCTTCACCTACCGAGTTCAAGTGATTTTCCTGCCTTGGCCTCCCAAGTAGCT GGGATTACAGGTGTGTGCCACCACACCCGGCTAATTTTTATATTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTAAAACTCCTGACCT CAAGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTGAGATTACAGGCATGAGCCACTGCACTCAACCTTAAGACCTACTGTTCTAAAGCTCTGACATTAT GTGGTTTTAGATTTTCTGGTTCTAACATTTTTGATAAAGCCTCAAGGTTTTAGGTTCTAAAGTTCTAAGATTCTGATTTTAGGAGCTAAGGCTCTATGAG TCTAGATGTTTATTCTTCTAGAGTTCAGAGTCCTTAAAATGTAAGATTATAGATTCTAAAGATTCTATAGTTCTAGACATGGAGGTTCTAAGGCCTAGGA TTCTAAAATGTGATGTTCTAAGGCTCTGAGAGTCTAGATTCTCTGGCTGTAAGGCTCTAGATCATAAGGCTTCAAAATGTTATCTTCTCAAGTTCTAAGA TTCTAATGATGATCAATTATAGTTTCTGAGGCTTTATGATAATAGATTCTCTTGTATAAGATCCTAGATCCTAAGGGTCGAAAGCTCTAGAATCTGCAAT TCAAAAGTTCCAAGAGTCTAAAGATGGAGTTTCTAAGGTCCGGTGTTCTAAGATGTGATATTCTAAGACTTACTCTAAGATCTTAGATTCTCTGTGTCTA AGATTCTAGATCAGATGCTCCAAGATTCTAGATGATTAAATAAGATTCTAACGGTCTGTTCTGTTTCAAGGCACTCTAGATTCCATTGGTCCAAGATTCC GGATCCTAAGCATCTAAGTTATAAGACTCTCACACTCAGTTGTGACTAACTAGACACCAAAGTTCTAATAATTTCTAATGTTGGACACCTTTAGGTTCTT TGCTGCATTCTGCCTCTCTAGGACCATGGTTAAGAGTCCAAGAATCCACATTTCTAAAATCTTATAGTTCTAGGCACTGTAGTTCTAAGACTCAAATGTT CTAAGTTTCTAAGATTCTAAAGGTCCACAGGTCTAGACTATTAGGTGCAATTTCAAGGTTCTAACCCTATACTGTAGTATTCTTTGGGGTGCCCCTCTCC TTCTTAGCTATCATTGCTTCCTCCTCCCCAACTGTGGGGGTGTGCCCCCTTCAAGCCTGTGCAATGCATTAGGGATGCCTCCTTTCCCGCAGGGGATGGA CGATCTCCCACCTTTCGGGCCATGTTGCCCCCGTGAGCCAATCCCTCACCTTCTGAGTACAGAGTGTGGACTCTGGTGCCTCCAGAGGGGCTCAGGTCAC ATAAAACTTTGTATATCAACGA

refseq_AXL.F1 (forward primer)
refseq_AXL.R1 (reverse primer)
Single exon skipping

Frame 0

EWSSGGMFTRHRAQRDSAQSPDRETRPHWLRTG GTATRVPFPLLSSLPGPFKKELILSSLELTPDCP GGPWALALVGGGKGGARGGERVAQVWE*GKEAG VLRRRLLGRAGGKGLPCRCARQAVPNPGSLELG GGPGTARPCPLPRWEPNNF*GKFGTHGVAVPQDG QGPAGLVLGAVRLGVHGPQGHAG*RKSLRGQPR EYHRCPGTHGHPSVSAPGSGRAPRGTLASGWTD PGARGQHPDPGAPG*G*TG*LDSGQPAQNHLPAA FRHGTVPVFGVSGTSDLRVPAWLCWAGGLALLP GGARRQDCGRQHPLQPELPSSGTPRARGPTLAP GCCPPGHGSRSRPPAQPACSRAEQDILFLLRSP* RQGGHHIPHSHHHSAPPAAP*PPPGLPPTHGAG GGLDSRPERHLPPDPLHPAGCAVKRWDGHPGGR TRPPRGAPHLASIRAPPSASARQPPSSHPLSHPR GMHQQPGPLILDPLASCGDAGGSAPGPP*EH*C YAEWEPGLRALARAPGAPAGYPVRVPAGVSRPG HPRGANGHRAKARGDPGAAGGRVCVQSDSVCGSL HCCWGWTLEPPSTPGGLAPRASTASPPAGEGTF NSCLLVALVVCTARSSRGRCLCPHLGSLPCPPA KEGDPLWRSV*TNSGKR*TGSQVPRAQVLQSSDH *SYLEQPGHQ*RAEGEAAGCDGGPAQGGPGEDS GRGRVWSCDGRPAQPGRLHPQGGCEDDEDCHLH EVRAGGFPE*SGLHEGI*PSQRHEAHRCLFPGF* TRELPSTCGHLTFHETWRPTQLPPLFPARGPAS VPAHSDASEVHGRHRQWHGVSEYQEIHTPGPGG QELHAE*EHVRVCGGLRALQEDLQWGLLPPGTYR QDASQVDCH*ESS*PCLHQQERCVVLRGDNVGD CHKRPNPISGRGEQRDL*LSAPGKSPEAACGLS GWTVCLDVAVLGAKSPGPAKFYRAAGRFGEHTEG LASCPGA*RNPLCQHG*GWRLS*TPWSCRRS*P PNPARP*GFL*LPHCG*GPSCWTLCPLPFHNP* PRSAC**GLPSSPRAGGWCLRQPSTWYSLSGSKL STATGENSTFPLSHPTPYPHLQPCLPTYPTSIP DRSLPFSVQ*HHLESSSITICKRKGLDCNI*SP PRC*HSKTLESKV*RV*IQRF*VSKML*VFGSKD LKFQSL*FY*SAKVLRPTFFFFFFFFFFAIESH CVTQAGVQWCNLASLQPSPTEFK*FSCLGLPSS WDYRCVPPHPANFYIFSRDRVSPCWPGWSKTPDL K*SAHLSLPKC*DYRHEPLHSTLRPTVLKL*HY VVLDFLVLTFLIKPQGFRF*SSKILILGAKAL* V*MFILLEFRVLKM*DYRF*RFYSSRHGGSKA*D SKM*CSKALRV*ILWL*GSRS*GFKMLSSQVLR F***SIIVSEAL***ILLYKILDPKGRKL*NLQ FKSSKSLKMEFLRSGVLRCDILRLTLRS*ILCV* DSRSDAPRF*MIK*DSNGLFCFKAL*IPLVQDS GS*ASKL*DSHTQL*LTRHQSSNNF*CWTPLGS LLHSASLGPWLRVQESTFLKSYSSRHCSSKTQMF *VSKILKVHRSRLLGAISRF*PYTVVFFGVPLS FLAIIASSSPTVGVCPLQACAMH*GCLLSRRGW TISHLSGHVAPVSQSLTF*VQSVDSGASRGAQVT *NFVYQR

Frame 1

SGVLEECLPDTEPRGTAPRARIERHGLTGSGQG AQPPGSPSPSSAPSLAPLRKS*SSPLLS*PLIV QVAPGLWPWWAEAKGEPGAEKGLPKSGSEGRRQG C*EGGCWAEPVARASPAAVPGRQCQIRGAWSWG EGRGQPGPAPSPAGSPTTSEESLAPMAWRCPRM GRVPLAWCLALCGWACMAPRGTQAEESPFVGNPG NITGARGLTGTLRCQLQVQGEPPEVHWLRDGQI LELADSTQTQVPLGEDEQDDWIVVSQLRITSLQ LSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFL EEPEDRTVAANTPFNLSCQAQGPPEPVDLLWLQ DAVPLATAPGHGPQRSLHVPGLNKTSSFSCEAH NAKGVTTSRTATITVLPQQPRNLHLVSRQPTELE VAWTPGLSGIYPLTHCTLQAVLSNDGMGIQAGE PDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIR VACTSSQGPSSWTHWLPVETPEGVPLGPPENISA TRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQD TPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAA YTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPS TPAFSWPWWYVLLGAVVAAACVLILALFLVHRR KKETRYGEVFEPTVERGELVVRYRVRKSYSRRT TEATLNSLGISEELKEKLRDVMVDRHKVALGKTL GEGEFGAVMEGQLNQDDSILKVAVKTMKIAICT RSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGS ERESFPAPVVILPFMKHGDLHSFLLYSRLGDQPV YLPTQMLVKFMADIASGMEYLSTKRFIHRDLAA RNCMLNENMSVCVADFGLSKKIYNGDYYRQGRI AKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEI ATRGQTPYPGVENSEIYDYLRQGNRLKQPADCL DGLYALMSRCWELNPQDRPSFTELREDLENTLK ALPPAQEPDEILYVNMDEGGGYPEPPGAAGGADP PTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPS PAQPADRGSPAAPGQEDGA*DNPPPGTPSQDPS *ALPLGKTPPSHFPTPRLIPTCSPVFLPIPPPSQ TGPSPSLCSSITLKAVASPSVKGRGWIAISEAL PGVNIPRL*SPRFKESRFKGSRFQRCCESLVLR T*NSKVSNSIKVLRF*GLLFFFFFFFFFLR*SLT VSPRLECSGAISPHCNLHLPSSSDFPALASQVA GITGVCHHTRLIFIFLVETGFHHVGQAGLKLLT SSDLPTSASQSAEITGMSHCTQP*DLLF*SSDIM WF*IFWF*HF**SLKVLGSKVLRF*F*ELRLYE SRCLFF*SSESLKCKIIDSKDSIVLDMEVLRPR ILKCDVLRL*ESRFSGCKALDHKASKCYLLKF*D SNDDQL*FLRLYDNRFSCIRS*ILRVESSRICN SKVPRV*RWSF*GPVF*DVIF*DLL*DLRFSVS KILDQMLQDSR*LNKILTVCSVSRHSRFHWSKIP DPKHLSYKTLTLSCD*LDTKVLIISNVGHL*VL CCILPL*DHG*ESKNPHF*NLIVLGTVVLRLKC SKFLRF*RSTGLDY*VQFQGSNPIL*YSLGCPSP S*LSLLPPPQLWGCAPFKPVQCIRDASFPAGDG RSPTFRAMLPP*ANPSPSEYRVWTLVPPEGLRS HKTLYIN

Frame 2

VEFWRNVYQTQSPEGQRPEPG*RDTASLAQDRG HSHQGPLPPPQLPPWPL*ERADPLLS*VNP*LS RWPLGSGPGGRRQRGSQGRRKGCPSLGVREGGR GAEKAAAGQSRWQGPPLPLCQAGSAKSGEPGAGG RAGDSPALPPPPLGAQQLLRKVWHPWRGGAPGW AGSRWPGAWRCAAGRAWPPGARRLKKVPSWATQ GISQVPGDSRAPFGVSSRFRESPPRYIGFGMDRS WSSRTAPRPRCPWVRMNRMTG*WSASSESPPCS FPTRDSTSVWCFWDIRPSCPSLAMLGWRACLTS WRSPKTGLWPPTPPST*AAKLRDPQSPWTYSGSR MLSPWPRLQVTAPSAACMFQG*TRHPLSPAKPI TPRGSPHPAQPPSQCSPSSPVTSTWSPANPRSW RWLGLQA*AASTP*PTAPCRLCCQTMGWASRREN QTPQRSPSPRKHPCPPISFG*AASILTPLITSA WHAPAARAPHPGPTGFLWRRRRECPWAPLRTLV LRGMGARPSCIGKSPGRPCRVPC*GTGWRIKART PQRC*WT*G*GKR*PWSCRGTGLCPI*QCVWQP TLLLGMDPGASQYPWRPGAQGKHSQSTSW*RNL QLLPSRGPGGMYC*EQSWPLPVSSSWLSSLSTGE RRRPVMEKCLNQQWKEVNW*SGTACASPTVVGP LKLP*TAWASVKS*RRSCGM*WWTGTRWPWGRL WERESLEL*WKASSTRTTPSSRWL*RR*RLPSAR GQSWRIS*VKRSA*RNLTIPTS*GSSVSVSRVL NERASQHLWSSYLS*NMETYTASSSIPGSGTSQ CTCPLRC**SSWQTSPVAWSI*VPRDSYTGTWRP GTAC*MRTCPCVWRTSGSPRRSTMGTTTARDVS PRCQSSGLPLRV*LTVSTPARAMCGPSG*QCGR LPQEAKPHIRAWRTARFMTICAREIA*SSLRTVW MDCMP*CRGAGS*IPRTGQVLQSCGKIWRTH*R PCLLPRSLTKSSMSTWMRVEVILNPLELQEELT PQPSQTLRIPVAASLRLRSILLDAMSSALPQPLA PLSLLIGAPQQPQGRRMVPETTLHLVLPLRIQA KHCHWGKLHLPTFPPHALSPLAALSSYLSHLHP RQVPPLLCAVASP*KQ*HHHL*KEGVGLQYLKPS QVLTFQDSRVQGLKSLDSKVLGFKDAVSLWF*G PEIPKSLILLKC*GSKAYFFFFFFFFFFCDRVS LCHPGWSAVVQSRLTATFTYRVQVIFLPWPPK*L GLQVCATTPG*FLYF**RQGFTMLARLV*NS*P QVICPPQPPKVLRLQA*ATALNLKTYCSKALTL CGFRFSGSNIFDKASRF*VLKF*DSDFRS*GSMS LDVYSSRVQSP*NVRL*ILKIL*F*TWRF*GLG F*NVMF*GSESLDSLAVRL*IIRLQNVIFSSSK ILMMINYSF*GFMIIDSLV*DPRS*GSKALESAI QKFQESKDGVSKVRCSKM*YSKTYSKILDSLCL RF*IRCSKILDD*IRF*RSVLFQGTLDSIGPRF RILSI*VIRLSHSVVTN*TPKF**FLMLDTFRFF AAFCLSRTMVKSPRIHISKIL*F*AL*F*DSNV LSF*DSKGPQV*TIRCNFKVLTLYCSILWGAPL LLSYHCFLLPNCGGVPPSSLCNALGMPPFPQGMD DLPPFGPCCPREPIPHLLSTECGLWCLQRGSGH IKLCIST