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Sequences

Sequence NM_001033855

GCTTCCCGGAAGTGGCGGCGCGGTCAGGGCTGGCCTTGGCTTCAGCTGCGGTTTTGGGGTCCCGGACTCTGGGATCGGCGGCGCTATGAGTTCTTTCGAG
GGGCAGATGGCCGAGTATCCAACTATCTCCATAGACCGCTTCGATAGGGAGAACCTGAGGGCCCGCGCCTACTTCCTGTCCCACTGCCACAAAGATCACA
TGAAAGGATTAAGAGCCCCTACCTTGAAAAGAAGGTTGGAGTGCAGCTTGAAGGTTTATCTATACTGTTCACCTGTGACTAAGGAGTTGTTGTTAACGAG
CCCGAAATACAGATTTTGGAAGAAACGAATTATATCTATTGAAATCGAGACTCCTACCCAGATATCTTTAGTGGATGAAGCATCAGGAGAGAAGGAAGAG
ATTGTTGTGACTCTCTTACCAGCTGGTCACTGTCCGGGATCAGTTATGTTTTTATTTCAGGGCAATAATGGAACTGTCCTGTACACAGGAGACTTCAGAT
TGGCGCAAGGAGAAGCTGCTAGAATGGAGCTTCTGCACTCCGGGGGCAGAGTCAAAGACATCCAAAGTGTATATTTGGATACTACGTTCTGTGATCCAAG
ATTTTACCAAATTCCAAGTCGGGAGGAGTGTTTAAGTGGAGTCTTAGAGCTGGTCCGAAGCTGGATCACTCGGAGCCCGTACCATGTTGTGTGGCTGAAC
TGCAAAGCGGCTTATGGCTATGAATATCTGTTCACCAACCTTAGTGAAGAATTAGGAGTCCAGGTTCATGTGAATAAGCTAGACATGTTTAGGAACATGC
CTGAGATCCTTCATCATCTCACAACAGACCGCAACACTCAGATCCATGCATGCCGGCATCCCAAGGCAGAGGAATATTTTCAGTGGAGCAAATTACCCTG
TGGAATTACTTCCAGAAATAGAATTCCACTCCACATAATCAGCATTAAGCCATCCACCATGTGGTTTGGAGAAAGGAGCAGAAAAACAAATGTAATTGTG
AGGACTGGAGAGAGTTCATACAGAGCTTGTTTTTCTTTTCACTCCTCCTACAGTGAGATTAAAGATTTCTTGAGCTACCTCTGTCCTGTGAACGCATATC
CAAATGTCATTCCAGTTGGCACAACTATGGATAAAGTTGTCGAAATCTTAAAGCCTTTATGCCGGTCTTCCCAAAGTACGGAGCCAAAGTATAAACCACT
GGGAAAACTGAAGAGAGCTAGAACAGTTCACCGAGACTCAGAGGAGGAAGATGACTATCTCTTTGATGATCCTCTGCCAATACCTTTAAGGCACAAAGTT
CCATACCCGGAAACTTTTCACCCTGAGGTATTTTCAATGACTGCAGTATCAGAAAAGCAGCCTGAAAAACTGAGACAAACCCCAGGATGCTGCAGAGCAG
AGTGTATGCAGAGCTCTCGTTTCACAAACTTTGTAGATTGTGAAGAATCCAACAGTGAAAGTGAAGAAGAAGTAGGAATCCCAGCTTCACTGCAAGGAGA
TCTGGGCTCTGTACTTCACCTGCAAAAGGCTGATGGGGATGTACCCCAGTGGGAAGTATTCTTTAAAAGAAATGATGAAATCACAGATGAGAGTTTGGAA
AACTTCCCTTCCTCCACAGTGGCAGGGGGATCTCAGTCACCAAAGCTTTTCAGTGACTCTGATGGAGAATCAACTCACATCTCCTCCCAGAATTCTTCCC
AGTCAACACACATAACAGAACAAGGAAGTCAAGGCTGGGACAGCCAATCTGATACTGTTTTGTTATCTTCCCAAGAGAGAAACAGTGGGGATATTACTTC
CTTGGACAAAGCTGACTACAGACCAACAATCAAAGAGAATATTCCTGCCTCTCTCATGGAACAAAATGTAATTTGCCCAAAGGATACTTACTCTGATTTG
AAAAGCAGAGATAAAGATGTGACAATAGTTCCTAGTACTGGAGAACCAACTACTCTAAGCAGTGAGACACATATACCCGAGGAAAAAAGTTTGCTAAATC
TTAGCACAAATGCAGATTCCCAGAGCTCTTCTGATTTTGAAGTTCCCTCAACTCCAGAAGCTGAGTTACCTAAACGAGAGCATTTACAATATTTATATGA
GAAGCTGGCAACTGGTGAGAGTATAGCAGTCAAAAAAAGAAAATGCTCACTCTTAGATACCTAAGAATTCAAAGCGTTTCAACCTAGAGCAACCACTAAA
AAACCTGCACAGAGATGACAGTCAATATTACAATAGAGAAAATACAGTACTTAAAAATGTTCAAATAACCTGGTTGGGTGTGGTGGCTCACACTTGTAAT
CCCAGCACTTTGAGGTGGGCAATGGCTTGAGCCCAGGAGTTCGACACCAGCCTGGCCAACACAGTGAAATGTGTCTCTACTTACAAAAAAAAAAAAAAAA
AAAAAAGCCAGGCATGGTGGTGGGCTACTCTGGAGGCCCTTGAGGATCACTTGAGCCCAGGAGATTGAGGCTGCAGTGAGCCATGACTGTGCCACTGCAC
CTCTAGTCTGGGTGACAGAGCAAGACCCTGTTTAAAAAAGAAAAAGGCCAGGCACGGTGGCTCATGCCTGTAATCCCAGCTACGCAGGAGGCCAAGGCAG
GAGAATCACTTGAACCTGGGAGGTGGTGGTTGCAGTGAGCTGAGATTGTGTCATTGCACTCCAGCCTGGGCAACAAGAGCAAAACTCTGTCTCAAAAAAA
AAAAAAAAAAAAAAGAAAAAAAAATGGTAGAACTAAACCCATACGTATCAATGATTACATTAAATGTAAATGGTCTAAGTGCATCAATGACAAGAGACAC
GGAAAAAACCAACATAACCCAGTGATCTGCTGTCTATAAAGAAAGTCAATTCAAGTGATTGATAGGTTAAAAGGATGGAAAAACCATGTAAACAATTAAA
AATGGAGTGGTTATATGGACAGTTTCCTAATGATAAAAGGTCTAGTTCACCAAGAAGACAAAATAATCCTAAACGTATATGCACCTAACAGCTCCAAAAA
TTTATGAAGCTAGAACAGCTGAAAAGAGACAAACTAATGCACAATTACAGCTAGGGATTAATTTAACATCCCTCCTTAGTAACAGAACTAGTATATACAC
ATTAATCAGTAAGAATATAGAACTGAAAATCATAAGCCGACTGGATTAAATTGACATTTATAGAACACACCACCCCAAACTAGCAGAATGTATCTCCTGA
AGTACACATGGAACATTCAGCATGATCATATCTTTGCTCATAAACACACCTGATTTAAATTGACAAACTTGGATCTAGTATGATAAACTGATGGCTATAG
ACAAAATCAGCACCTTATTTATCCCCAAAGATGTTAAGTCACTTAACCTTGATGTGAATACGGTGGCCAAATTAGAAGCGGACAATTAGAATATAAAAGA
AAATGCTCAAGAATTAAATGGTGGCCAGGCACAGTAACTCCTGCCTGCAATCCCAGTACTTTGGGAGGCTCGCTTGAGCTCAGGGTTTTGAGTCCAGCCT
GGGCAACACACAGGGAGACCCTGTCTCCACAAAATGTTAGCCAGACATGGTGGTGTGCACCTGTAGTCCTCGCTACTTGGAAGGCTTAGGTGGCAGGATG
GATTGAGCCCAGGAGGTTGAAACTGCAGTGAGCTGATTGTGCCCAGCCTGGGCAACAAAGCAAGACCCCATCTCAAGAAAAATAAATTAAAAAAAACTAA
A
  • refseq_DCLRE1C.F3 (forward primer)
  • refseq_DCLRE1C.R3 (reverse primer)
  • Single exon skipping

Frame 0

ASRKWRRGQGWPWLQLRFWGPGLWDRRRYEFFR
GADGRVSNYLHRPLR*GEPEGPRLLPVPLPQRSH
ERIKSPYLEKKVGVQLEGLSILFTCD*GVVVNE
PEIQILEETNYIY*NRDSYPDIFSG*SIRREGR
DCCDSLTSWSLSGISYVFISGQ*WNCPVHRRLQI
GARRSC*NGASALRGQSQRHPKCIFGYYVL*SK
ILPNSKSGGVFKWSLRAGPKLDHSEPVPCCVAE
LQSGLWL*ISVHQP**RIRSPGSCE*ARHV*EHA
*DPSSSHNRPQHSDPCMPASQGRGIFSVEQITL
WNYFQK*NSTPHNQH*AIHHVVWRKEQKNKCNC
EDWREFIQSLFFFSLLLQ*D*RFLELPLSCERIS
KCHSSWHNYG*SCRNLKAFMPVFPKYGAKV*TT
GKTEES*NSSPRLRGGR*LSL**SSANTFKAQS
SIPGNFSP*GIFNDCSIRKAA*KTETNPRMLQSR
VYAELSFHKLCRL*RIQQ*K*RRSRNPSFTARR
SGLCTSPAKG*WGCTPVGSIL*KK**NHR*EFG
KLPFLHSGRGISVTKAFQ*L*WRINSHLLPEFFP
VNTHNRTRKSRLGQPI*YCFVIFPREKQWGYYF
LGQS*LQTNNQREYSCLSHGTKCNLPKGYLL*F
EKQR*RCDNSS*YWRTNYSKQ*DTYTRGKKFAKS
*HKCRFPELF*F*SSLNSRS*VT*TRAFTIFI*
EAGNW*EYSSQKKKMLTLRYLRIQSVST*SNH*
KTCTEMTVNITIEKIQYLKMFK*PGWVWWLTLVI
PAL*GGQWLEPRSSTPAWPTQ*NVSLLTKKKKK
KKARHGGGLLWRPLRIT*AQEIEAAVSHDCATA
PLVWVTEQDPV*KRKRPGTVAHACNPSYAGGQGR
RIT*TWEVVVAVS*DCVIALQPGQQEQNSVSKK
KKKKKKKKW*N*THTYQ*LH*M*MV*VHQ*QET
RKKPT*PSDLLSIKKVNSSD**VKRMEKPCKQLK
MEWLYGQFPNDKRSSSPRRQNNPKRICT*QLQK
FMKLEQLKRDKLMHNYS*GLI*HPSLVTELVYT
H*SVRI*N*KS*ADWIKLTFIEHTTPN*QNVSPE
VHMEHSA*SYLCS*THLI*IDKLGSSMIN*WL*
TKSAPYLSPKMLSHLTLM*IRWPN*KRTIRI*K
KMLKN*MVARHSNSCLQSQYFGRLA*AQGFESSL
GNTQGDPVSTKC*PDMVVCTCSPRYLEGLGGRM
D*AQEVETAVS*LCPAWATKQDPISRKIN*KKL

Frame 1

LPGSGGAVRAGLGFSCGFGVPDSGIGGAMSSFE
GQMAEYPTISIDRFDRENLRARAYFLSHCHKDH
MKGLRAPTLKRRLECSLKVYLYCSPVTKELLLTS
PKYRFWKKRIISIEIETPTQISLVDEASGEKEE
IVVTLLPAGHCPGSVMFLFQGNNGTVLYTGDFR
LAQGEAARMELLHSGGRVKDIQSVYLDTTFCDPR
FYQIPSREECLSGVLELVRSWITRSPYHVVWLN
CKAAYGYEYLFTNLSEELGVQVHVNKLDMFRNM
PEILHHLTTDRNTQIHACRHPKAEEYFQWSKLPC
GITSRNRIPLHIISIKPSTMWFGERSRKTNVIV
RTGESSYRACFSFHSSYSEIKDFLSYLCPVNAY
PNVIPVGTTMDKVVEILKPLCRSSQSTEPKYKPL
GKLKRARTVHRDSEEEDDYLFDDPLPIPLRHKV
PYPETFHPEVFSMTAVSEKQPEKLRQTPGCCRA
ECMQSSRFTNFVDCEESNSESEEEVGIPASLQGD
LGSVLHLQKADGDVPQWEVFFKRNDEITDESLE
NFPSSTVAGGSQSPKLFSDSDGESTHISSQNSS
QSTHITEQGSQGWDSQSDTVLLSSQERNSGDITS
LDKADYRPTIKENIPASLMEQNVICPKDTYSDL
KSRDKDVTIVPSTGEPTTLSSETHIPEEKSLLN
LSTNADSQSSSDFEVPSTPEAELPKREHLQYLYE
KLATGESIAVKKRKCSLLDT*EFKAFQPRATTK
KPAQR*QSILQ*RKYST*KCSNNLVGCGGSHL*
SQHFEVGNGLSPGVRHQPGQHSEMCLYLQKKKKK
KKPGMVVGYSGGP*GSLEPRRLRLQ*AMTVPLH
L*SG*QSKTLFKKEKGQARWLMPVIPATQEAKA
GESLEPGRWWLQ*AEIVSLHSSLGNKSKTLSQKK
KKKKRKKNGRTKPIRINDYIKCKWSKCINDKRH
GKNQHNPVICCL*RKSIQVIDRLKGWKNHVNN*
KWSGYMDSFLMIKGLVHQEDKIILNVYAPNSSKN
L*S*NS*KETN*CTITARD*FNIPP**QN*YIH
INQ*EYRTENHKPTGLN*HL*NTPPQTSRMYLL
KYTWNIQHDHIFAHKHT*FKLTNLDLV**TDGYR
QNQHLIYPQRC*VT*P*CEYGGQIRSGQLEYKR
KCSRIKWWPGTVTPACNPSTLGGSLELRVLSPA
WATHRETLSPQNVSQTWWCAPVVLATWKA*VAGW
IEPRRLKLQ*ADCAQPGQQSKTPSQEK*IKKN*

Frame 2

FPEVAARSGLALASAAVLGSRTLGSAAL*VLSR
GRWPSIQLSP*TASIGRT*GPAPTSCPTATKIT
*KD*EPLP*KEGWSAA*RFIYTVHL*LRSCC*R
ARNTDFGRNELYLLKSRLLPRYL*WMKHQERRKR
LL*LSYQLVTVRDQLCFYFRAIMELSCTQETSD
WRKEKLLEWSFCTPGAESKTSKVYIWILRSVIQ
DFTKFQVGRSV*VES*SWSEAGSLGARTMLCG*T
AKRLMAMNICSPTLVKN*ESRFM*IS*TCLGTC
LRSFIISQQTATLRSMHAGIPRQRNIFSGANYP
VELLPEIEFHST*SALSHPPCGLEKGAEKQM*L*
GLERVHTELVFLFTPPTVRLKIS*ATSVL*THI
QMSFQLAQLWIKLSKS*SLYAGLPKVRSQSINH
WEN*RELEQFTETQRRKMTISLMILCQYL*GTKF
HTRKLFTLRYFQ*LQYQKSSLKN*DKPQDAAEQ
SVCRALVSQTL*IVKNPTVKVKKK*ESQLHCKE
IWALYFTCKRLMGMYPSGKYSLKEMMKSQMRVWK
TSLPPQWQGDLSHQSFSVTLMENQLTSPPRILP
SQHT*QNKEVKAGTANLILFCYLPKRETVGILL
PWTKLTTDQQSKRIFLPLSWNKM*FAQRILTLI*
KAEIKM*Q*FLVLENQLL*AVRHIYPRKKVC*I
LAQMQIPRALLILKFPQLQKLSYLNESIYNIYM
RSWQLVRV*QSKKENAHS*IPKNSKRFNLEQPLK
NLHRDDSQYYNRENTVLKNVQITWLGVVAHTCN
PSTLRWAMA*AQEFDTSLANTVKCVSTYKKKKK
KKSQAWWWATLEALEDHLSPGD*GCSEP*LCHCT
SSLGDRARPCLKKKKARHGGSCL*SQLRRRPRQ
ENHLNLGGGGCSELRLCHCTPAWATRAKLCLKK
KKKKKEKKMVELNPYVSMITLNVNGLSASMTRDT
EKTNITQ*SAVYKESQFK*LIG*KDGKTM*TIK
NGVVIWTVS***KV*FTKKTK*S*TYMHLTAPK
IYEARTAEKRQTNAQLQLGINLTSLLSNRTSIYT
LISKNIELKIISRLD*IDIYRTHHPKLAECIS*
STHGTFSMIISLLINTPDLN*QTWI*YDKLMAI
DKISTLFIPKDVKSLNLDVNTVAKLEADN*NIKE
NAQELNGGQAQ*LLPAIPVLWEARLSSGF*VQP
GQHTGRPCLHKMLARHGGVHL*SSLLGRLRWQD
GLSPGG*NCSELIVPSLGNKARPHLKKNKLKKTK

Sequence NM_022487

GCTTCCCGGAAGTGGCGGCGCGGTCAGGGCTGGCCTTGGCTTCAGCTGCGGTTTTGGGGTCCCGGACTCTGGGATCGGCGGCGCTATGAGTTCTTTCGAG
GGGCAGATGGCCGAGTATCCAACTATCTCCATAGACCGCTTCGATAGGGAGAACCTGAGGGCCCGCGCCTACTTCCTGTCCCACTGCCACAAAGATCACA
TGAAAGGATTAAGAGCCCCTACCTTGAAAAGAAGGTTGGAGTGCAGCTTGAAGGTTTATCTATACTGTTCACCTGTGACTAAGGAGTTGTTGTTAACGAG
CCCGAAATACAGATTTTGGAAGAAACGAATTATATCTATTGAAATCGAGACTCCTACCCAGATATCTTTAGTGGATGAAGCATCAGGAGAGGTTTTTATT
TCAGGGCAATAATGGAACTGTCCTGTACACAGGAGACTTCAGATTGGCGCAAGGAGAAGCTGCTAGAATGGAGCTTCTGCACTCCGGGGGCAGAGTCAAA
GACATCCAAAGTGTATATTTGGATACTACGTTCTGTGATCCAAGATTTTACCAAATTCCAAGTCGGGAGGAGTGTTTAAGTGGAGTCTTAGAGCTGGTCC
GAAGCTGGATCACTCGGAGCCCGTACCATGTTGTGTGGCTGAACTGCAAAGCGGCTTATGGCTATGAATATCTGTTCACCAACCTTAGTGAAGAATTAGG
AGTCCAGGTTCATGTGAATAAGCTAGACATGTTTAGGAACATGCCTGAGATCCTTCATCATCTCACAACAGACCGCAACACTCAGATCCATGCATGCCGG
CATCCCAAGGCAGAGGAATATTTTCAGTGGAGCAAATTACCCTGTGGAATTACTTCCAGAAATAGAATTCCACTCCACATAATCAGCATTAAGCCATCCA
CCATGTGGTTTGGAGAAAGGAGCAGAAAAACAAATGTAATTGTGAGGACTGGAGAGAGTTCATACAGAGCTTGTTTTTCTTTTCACTCCTCCTACAGTGA
GATTAAAGATTTCTTGAGCTACCTCTGTCCTGTGAACGCATATCCAAATGTCATTCCAGTTGGCACAACTATGGATAAAGTTGTCGAAATCTTAAAGCCT
TTATGCCGGTCTTCCCAAAGTACGGAGCCAAAGTATAAACCACTGGGAAAACTGAAGAGAGCTAGAACAGTTCACCGAGACTCAGAGGAGGAAGATGACT
ATCTCTTTGATGATCCTCTGCCAATACCTTTAAGGCACAAAGTTCCATACCCGGAAACTTTTCACCCTGAGGTATTTTCAATGACTGCAGTATCAGAAAA
GCAGCCTGAAAAACTGAGACAAACCCCAGGATGCTGCAGAGCAGAGTGTATGCAGAGCTCTCGTTTCACAAACTTTGTAGATTGTGAAGAATCCAACAGT
GAAAGTGAAGAAGAAGTAGGAATCCCAGCTTCACTGCAAGGAGATCTGGGCTCTGTACTTCACCTGCAAAAGGCTGATGGGGATGTACCCCAGTGGGAAG
TATTCTTTAAAAGAAATGATGAAATCACAGATGAGAGTTTGGAAAACTTCCCTTCCTCCACAGTGGCAGGGGGATCTCAGTCACCAAAGCTTTTCAGTGA
CTCTGATGGAGAATCAACTCACATCTCCTCCCAGAATTCTTCCCAGTCAACACACATAACAGAACAAGGAAGTCAAGGCTGGGACAGCCAATCTGATACT
GTTTTGTTATCTTCCCAAGAGAGAAACAGTGGGGATATTACTTCCTTGGACAAAGCTGACTACAGACCAACAATCAAAGAGAATATTCCTGCCTCTCTCA
TGGAACAAAATGTAATTTGCCCAAAGGATACTTACTCTGATTTGAAAAGCAGAGATAAAGATGTGACAATAGTTCCTAGTACTGGAGAACCAACTACTCT
AAGCAGTGAGACACATATACCCGAGGAAAAAAGTTTGCTAAATCTTAGCACAAATGCAGATTCCCAGAGCTCTTCTGATTTTGAAGTTCCCTCAACTCCA
GAAGCTGAGTTACCTAAACGAGAGCATTTACAATATTTATATGAGAAGCTGGCAACTGGTGAGAGTATAGCAGTCAAAAAAAGAAAATGCTCACTCTTAG
ATACCTAAGAATTCAAAGCGTTTCAACCTAGAGCAACCACTAAAAAACCTGCACAGAGATGACAGTCAATATTACAATAGAGAAAATACAGTACTTAAAA
ATGTTCAAATAACCTGGTTGGGTGTGGTGGCTCACACTTGTAATCCCAGCACTTTGAGGTGGGCAATGGCTTGAGCCCAGGAGTTCGACACCAGCCTGGC
CAACACAGTGAAATGTGTCTCTACTTACAAAAAAAAAAAAAAAAAAAAAAGCCAGGCATGGTGGTGGGCTACTCTGGAGGCCCTTGAGGATCACTTGAGC
CCAGGAGATTGAGGCTGCAGTGAGCCATGACTGTGCCACTGCACCTCTAGTCTGGGTGACAGAGCAAGACCCTGTTTAAAAAAGAAAAAGGCCAGGCACG
GTGGCTCATGCCTGTAATCCCAGCTACGCAGGAGGCCAAGGCAGGAGAATCACTTGAACCTGGGAGGTGGTGGTTGCAGTGAGCTGAGATTGTGTCATTG
CACTCCAGCCTGGGCAACAAGAGCAAAACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAATGGTAGAACTAAACCCATACGTATCAATGATT
ACATTAAATGTAAATGGTCTAAGTGCATCAATGACAAGAGACACGGAAAAAACCAACATAACCCAGTGATCTGCTGTCTATAAAGAAAGTCAATTCAAGT
GATTGATAGGTTAAAAGGATGGAAAAACCATGTAAACAATTAAAAATGGAGTGGTTATATGGACAGTTTCCTAATGATAAAAGGTCTAGTTCACCAAGAA
GACAAAATAATCCTAAACGTATATGCACCTAACAGCTCCAAAAATTTATGAAGCTAGAACAGCTGAAAAGAGACAAACTAATGCACAATTACAGCTAGGG
ATTAATTTAACATCCCTCCTTAGTAACAGAACTAGTATATACACATTAATCAGTAAGAATATAGAACTGAAAATCATAAGCCGACTGGATTAAATTGACA
TTTATAGAACACACCACCCCAAACTAGCAGAATGTATCTCCTGAAGTACACATGGAACATTCAGCATGATCATATCTTTGCTCATAAACACACCTGATTT
AAATTGACAAACTTGGATCTAGTATGATAAACTGATGGCTATAGACAAAATCAGCACCTTATTTATCCCCAAAGATGTTAAGTCACTTAACCTTGATGTG
AATACGGTGGCCAAATTAGAAGCGGACAATTAGAATATAAAAGAAAATGCTCAAGAATTAAATGGTGGCCAGGCACAGTAACTCCTGCCTGCAATCCCAG
TACTTTGGGAGGCTCGCTTGAGCTCAGGGTTTTGAGTCCAGCCTGGGCAACACACAGGGAGACCCTGTCTCCACAAAATGTTAGCCAGACATGGTGGTGT
GCACCTGTAGTCCTCGCTACTTGGAAGGCTTAGGTGGCAGGATGGATTGAGCCCAGGAGGTTGAAACTGCAGTGAGCTGATTGTGCCCAGCCTGGGCAAC
AAAGCAAGACCCCATCTCAAGAAAAATAAATTAAAAAAAACTAAA
  • refseq_DCLRE1C.F3 (forward primer)
  • refseq_DCLRE1C.R3 (reverse primer)
  • Single exon skipping

Frame 0

ASRKWRRGQGWPWLQLRFWGPGLWDRRRYEFFR
GADGRVSNYLHRPLR*GEPEGPRLLPVPLPQRSH
ERIKSPYLEKKVGVQLEGLSILFTCD*GVVVNE
PEIQILEETNYIY*NRDSYPDIFSG*SIRRGFY
FRAIMELSCTQETSDWRKEKLLEWSFCTPGAESK
TSKVYIWILRSVIQDFTKFQVGRSV*VES*SWS
EAGSLGARTMLCG*TAKRLMAMNICSPTLVKN*
ESRFM*IS*TCLGTCLRSFIISQQTATLRSMHAG
IPRQRNIFSGANYPVELLPEIEFHST*SALSHP
PCGLEKGAEKQM*L*GLERVHTELVFLFTPPTV
RLKIS*ATSVL*THIQMSFQLAQLWIKLSKS*SL
YAGLPKVRSQSINHWEN*RELEQFTETQRRKMT
ISLMILCQYL*GTKFHTRKLFTLRYFQ*LQYQK
SSLKN*DKPQDAAEQSVCRALVSQTL*IVKNPTV
KVKKK*ESQLHCKEIWALYFTCKRLMGMYPSGK
YSLKEMMKSQMRVWKTSLPPQWQGDLSHQSFSV
TLMENQLTSPPRILPSQHT*QNKEVKAGTANLIL
FCYLPKRETVGILLPWTKLTTDQQSKRIFLPLS
WNKM*FAQRILTLI*KAEIKM*Q*FLVLENQLL
*AVRHIYPRKKVC*ILAQMQIPRALLILKFPQLQ
KLSYLNESIYNIYMRSWQLVRV*QSKKENAHS*
IPKNSKRFNLEQPLKNLHRDDSQYYNRENTVLK
NVQITWLGVVAHTCNPSTLRWAMA*AQEFDTSLA
NTVKCVSTYKKKKKKKSQAWWWATLEALEDHLS
PGD*GCSEP*LCHCTSSLGDRARPCLKKKKARH
GGSCL*SQLRRRPRQENHLNLGGGGCSELRLCHC
TPAWATRAKLCLKKKKKKKEKKMVELNPYVSMI
TLNVNGLSASMTRDTEKTNITQ*SAVYKESQFK
*LIG*KDGKTM*TIKNGVVIWTVS***KV*FTKK
TK*S*TYMHLTAPKIYEARTAEKRQTNAQLQLG
INLTSLLSNRTSIYTLISKNIELKIISRLD*ID
IYRTHHPKLAECIS*STHGTFSMIISLLINTPDL
N*QTWI*YDKLMAIDKISTLFIPKDVKSLNLDV
NTVAKLEADN*NIKENAQELNGGQAQ*LLPAIP
VLWEARLSSGF*VQPGQHTGRPCLHKMLARHGGV
HL*SSLLGRLRWQDGLSPGG*NCSELIVPSLGN
KARPHLKKNKLKKTK

Frame 1

LPGSGGAVRAGLGFSCGFGVPDSGIGGAMSSFE
GQMAEYPTISIDRFDRENLRARAYFLSHCHKDH
MKGLRAPTLKRRLECSLKVYLYCSPVTKELLLTS
PKYRFWKKRIISIEIETPTQISLVDEASGEVFI
SGQ*WNCPVHRRLQIGARRSC*NGASALRGQSQ
RHPKCIFGYYVL*SKILPNSKSGGVFKWSLRAGP
KLDHSEPVPCCVAELQSGLWL*ISVHQP**RIR
SPGSCE*ARHV*EHA*DPSSSHNRPQHSDPCMP
ASQGRGIFSVEQITLWNYFQK*NSTPHNQH*AIH
HVVWRKEQKNKCNCEDWREFIQSLFFFSLLLQ*
D*RFLELPLSCERISKCHSSWHNYG*SCRNLKA
FMPVFPKYGAKV*TTGKTEES*NSSPRLRGGR*L
SL**SSANTFKAQSSIPGNFSP*GIFNDCSIRK
AA*KTETNPRMLQSRVYAELSFHKLCRL*RIQQ
*K*RRSRNPSFTARRSGLCTSPAKG*WGCTPVGS
IL*KK**NHR*EFGKLPFLHSGRGISVTKAFQ*
L*WRINSHLLPEFFPVNTHNRTRKSRLGQPI*Y
CFVIFPREKQWGYYFLGQS*LQTNNQREYSCLSH
GTKCNLPKGYLL*FEKQR*RCDNSS*YWRTNYS
KQ*DTYTRGKKFAKS*HKCRFPELF*F*SSLNS
RS*VT*TRAFTIFI*EAGNW*EYSSQKKKMLTLR
YLRIQSVST*SNH*KTCTEMTVNITIEKIQYLK
MFK*PGWVWWLTLVIPAL*GGQWLEPRSSTPAW
PTQ*NVSLLTKKKKKKKARHGGGLLWRPLRIT*A
QEIEAAVSHDCATAPLVWVTEQDPV*KRKRPGT
VAHACNPSYAGGQGRRIT*TWEVVVAVS*DCVI
ALQPGQQEQNSVSKKKKKKKKKKW*N*THTYQ*L
H*M*MV*VHQ*QETRKKPT*PSDLLSIKKVNSS
D**VKRMEKPCKQLKMEWLYGQFPNDKRSSSPR
RQNNPKRICT*QLQKFMKLEQLKRDKLMHNYS*G
LI*HPSLVTELVYTH*SVRI*N*KS*ADWIKLT
FIEHTTPN*QNVSPEVHMEHSA*SYLCS*THLI
*IDKLGSSMIN*WL*TKSAPYLSPKMLSHLTLM*
IRWPN*KRTIRI*KKMLKN*MVARHSNSCLQSQ
YFGRLA*AQGFESSLGNTQGDPVSTKC*PDMVV
CTCSPRYLEGLGGRMD*AQEVETAVS*LCPAWAT
KQDPISRKIN*KKL

Frame 2

FPEVAARSGLALASAAVLGSRTLGSAAL*VLSR
GRWPSIQLSP*TASIGRT*GPAPTSCPTATKIT
*KD*EPLP*KEGWSAA*RFIYTVHL*LRSCC*R
ARNTDFGRNELYLLKSRLLPRYL*WMKHQERFLF
QGNNGTVLYTGDFRLAQGEAARMELLHSGGRVK
DIQSVYLDTTFCDPRFYQIPSREECLSGVLELV
RSWITRSPYHVVWLNCKAAYGYEYLFTNLSEELG
VQVHVNKLDMFRNMPEILHHLTTDRNTQIHACR
HPKAEEYFQWSKLPCGITSRNRIPLHIISIKPS
TMWFGERSRKTNVIVRTGESSYRACFSFHSSYSE
IKDFLSYLCPVNAYPNVIPVGTTMDKVVEILKP
LCRSSQSTEPKYKPLGKLKRARTVHRDSEEEDD
YLFDDPLPIPLRHKVPYPETFHPEVFSMTAVSEK
QPEKLRQTPGCCRAECMQSSRFTNFVDCEESNS
ESEEEVGIPASLQGDLGSVLHLQKADGDVPQWE
VFFKRNDEITDESLENFPSSTVAGGSQSPKLFSD
SDGESTHISSQNSSQSTHITEQGSQGWDSQSDT
VLLSSQERNSGDITSLDKADYRPTIKENIPASL
MEQNVICPKDTYSDLKSRDKDVTIVPSTGEPTTL
SSETHIPEEKSLLNLSTNADSQSSSDFEVPSTP
EAELPKREHLQYLYEKLATGESIAVKKRKCSLL
DT*EFKAFQPRATTKKPAQR*QSILQ*RKYST*K
CSNNLVGCGGSHL*SQHFEVGNGLSPGVRHQPG
QHSEMCLYLQKKKKKKKPGMVVGYSGGP*GSLE
PRRLRLQ*AMTVPLHL*SG*QSKTLFKKEKGQAR
WLMPVIPATQEAKAGESLEPGRWWLQ*AEIVSL
HSSLGNKSKTLSQKKKKKKRKKNGRTKPIRIND
YIKCKWSKCINDKRHGKNQHNPVICCL*RKSIQV
IDRLKGWKNHVNN*KWSGYMDSFLMIKGLVHQE
DKIILNVYAPNSSKNL*S*NS*KETN*CTITAR
D*FNIPP**QN*YIHINQ*EYRTENHKPTGLN*H
L*NTPPQTSRMYLLKYTWNIQHDHIFAHKHT*F
KLTNLDLV**TDGYRQNQHLIYPQRC*VT*P*C
EYGGQIRSGQLEYKRKCSRIKWWPGTVTPACNPS
TLGGSLELRVLSPAWATHRETLSPQNVSQTWWC
APVVLATWKA*VAGWIEPRRLKLQ*ADCAQPGQ
QSKTPSQEK*IKKN*

Sequence NM_001033858

GCTTCCCGGAAGTGGCGGCGCGGTCAGGGCTGGCCTTGGCTTCAGCTGCGGTTTTGGGGTCCCGGACTCTGGGATCGGCGGCGCTATGAGTTCTTTCGAG
GGGCAGATGGCCGAGTATCCAACTATCTCCATAGACCGCTTCGATAGGGAGAACCTGAGGGCCCGCGCCTACTTCCTGTCCCACTGCCACAAAGATCACA
TGAAAGGATTAAGAGCCCCTACCTTGAAAAGAAGGTTGGAGTGCAGATGAGGAAACTGAGGAACCAGAGAGGTTAAATTAGTTGCCCAGTGTCACACAGC
CAGTAAGTGGCAGGACCAGGATTCCAGCCAGCTTGAAGGTTTATCTATACTGTTCACCTGTGACTAAGGAGTTGTTGTTAACGAGCCCGAAATACAGATT
TTGGAAGAAACGAATTAGCAGTCACAACAGCAGGGTTGTTCCTGTTGTCTCTGGCATTCAGCTTGAAGTTACTACAGGTCAGCCCGGCCAGCAGTCAGGA
AGGAAAGCTGGAAGAGAAGTGGAGGGGAACAGAGACAAACTGGAAGGCAGAATGGCCCACTAGGACAGACCGAAACCCACTTCTGTCTCTCACTTCCTCT
GGCCTCAATAGTGCTGGTCTCCCGCTTTGGCTCTGAAGCACATGCACCTGCCCCAGGACTCGGAGGAGCTGCACACGGGCACCCAGCAGAAGCTGATGTT
GCTGCGGCCCTGCCCCAGACTCTGAGTTAAGCCAGAAGATATCTATTGAAATCGAGACTCCTACCCAGATATCTTTAGTGGATGAAGCATCAGGAGAGAA
GGAAGAGATTGTTGTGACTCTCTTACCAGCTGGTCACTGTCCGGGATCAGTTATGTTTTTATTTCAGGGCAATAATGGAACTGTCCTGTACACAGGAGAC
TTCAGATTGGCGCAAGGAGAAGCTGCTAGAATGGAGCTTCTGCACTCCGGGGGCAGAGTCAAAGACATCCAAAGTGTATATTTGGATACTACGTTCTGTG
ATCCAAGATTTTACCAAATTCCAAGTCGGGAGGAGTGTTTAAGTGGAGTCTTAGAGCTGGTCCGAAGCTGGATCACTCGGAGCCCGTACCATGTTGTGTG
GCTGAACTGCAAAGCGGCTTATGGCTATGAATATCTGTTCACCAACCTTAGTGAAGAATTAGGAGTCCAGGTTCATGTGAATAAGCTAGACATGTTTAGG
AACATGCCTGAGATCCTTCATCATCTCACAACAGACCGCAACACTCAGATCCATGCATGCCGGCATCCCAAGGCAGAGGAATATTTTCAGTGGAGCAAAT
TACCCTGTGGAATTACTTCCAGAAATAGAATTCCACTCCACATAATCAGCATTAAGCCATCCACCATGTGGTTTGGAGAAAGGAGCAGAAAAACAAATGT
AATTGTGAGGACTGGAGAGAGTTCATACAGAGCTTGTTTTTCTTTTCACTCCTCCTACAGTGAGATTAAAGATTTCTTGAGCTACCTCTGTCCTGTGAAC
GCATATCCAAATGTCATTCCAGTTGGCACAACTATGGATAAAGTTGTCGAAATCTTAAAGCCTTTATGCCGGTCTTCCCAAAGTACGGAGCCAAAGTATA
AACCACTGGGAAAACTGAAGAGAGCTAGAACAGTTCACCGAGACTCAGAGGAGGAAGATGACTATCTCTTTGATGATCCTCTGCCAATACCTTTAAGGCA
CAAAGTTCCATACCCGGAAACTTTTCACCCTGAGGTATTTTCAATGACTGCAGTATCAGAAAAGCAGCCTGAAAAACTGAGACAAACCCCAGGATGCTGC
AGAGCAGAGTGTATGCAGAGCTCTCGTTTCACAAACTTTGTAGATTGTGAAGAATCCAACAGTGAAAGTGAAGAAGAAGTAGGAATCCCAGCTTCACTGC
AAGGAGATCTGGGCTCTGTACTTCACCTGCAAAAGGCTGATGGGGATGTACCCCAGTGGGAAGTATTCTTTAAAAGAAATGATGAAATCACAGATGAGAG
TTTGGAAAACTTCCCTTCCTCCACAGTGGCAGGGGGATCTCAGTCACCAAAGCTTTTCAGTGACTCTGATGGAGAATCAACTCACATCTCCTCCCAGAAT
TCTTCCCAGTCAACACACATAACAGAACAAGGAAGTCAAGGCTGGGACAGCCAATCTGATACTGTTTTGTTATCTTCCCAAGAGAGAAACAGTGGGGATA
TTACTTCCTTGGACAAAGCTGACTACAGACCAACAATCAAAGAGAATATTCCTGCCTCTCTCATGGAACAAAATGTAATTTGCCCAAAGGATACTTACTC
TGATTTGAAAAGCAGAGATAAAGATGTGACAATAGTTCCTAGTACTGGAGAACCAACTACTCTAAGCAGTGAGACACATATACCCGAGGAAAAAAGTTTG
CTAAATCTTAGCACAAATGCAGATTCCCAGAGCTCTTCTGATTTTGAAGTTCCCTCAACTCCAGAAGCTGAGTTACCTAAACGAGAGCATTTACAATATT
TATATGAGAAGCTGGCAACTGGTGAGAGTATAGCAGTCAAAAAAAGAAAATGCTCACTCTTAGATACCTAAGAATTCAAAGCGTTTCAACCTAGAGCAAC
CACTAAAAAACCTGCACAGAGATGACAGTCAATATTACAATAGAGAAAATACAGTACTTAAAAATGTTCAAATAACCTGGTTGGGTGTGGTGGCTCACAC
TTGTAATCCCAGCACTTTGAGGTGGGCAATGGCTTGAGCCCAGGAGTTCGACACCAGCCTGGCCAACACAGTGAAATGTGTCTCTACTTACAAAAAAAAA
AAAAAAAAAAAAAGCCAGGCATGGTGGTGGGCTACTCTGGAGGCCCTTGAGGATCACTTGAGCCCAGGAGATTGAGGCTGCAGTGAGCCATGACTGTGCC
ACTGCACCTCTAGTCTGGGTGACAGAGCAAGACCCTGTTTAAAAAAGAAAAAGGCCAGGCACGGTGGCTCATGCCTGTAATCCCAGCTACGCAGGAGGCC
AAGGCAGGAGAATCACTTGAACCTGGGAGGTGGTGGTTGCAGTGAGCTGAGATTGTGTCATTGCACTCCAGCCTGGGCAACAAGAGCAAAACTCTGTCTC
AAAAAAAAAAAAAAAAAAAAAGAAAAAAAAATGGTAGAACTAAACCCATACGTATCAATGATTACATTAAATGTAAATGGTCTAAGTGCATCAATGACAA
GAGACACGGAAAAAACCAACATAACCCAGTGATCTGCTGTCTATAAAGAAAGTCAATTCAAGTGATTGATAGGTTAAAAGGATGGAAAAACCATGTAAAC
AATTAAAAATGGAGTGGTTATATGGACAGTTTCCTAATGATAAAAGGTCTAGTTCACCAAGAAGACAAAATAATCCTAAACGTATATGCACCTAACAGCT
CCAAAAATTTATGAAGCTAGAACAGCTGAAAAGAGACAAACTAATGCACAATTACAGCTAGGGATTAATTTAACATCCCTCCTTAGTAACAGAACTAGTA
TATACACATTAATCAGTAAGAATATAGAACTGAAAATCATAAGCCGACTGGATTAAATTGACATTTATAGAACACACCACCCCAAACTAGCAGAATGTAT
CTCCTGAAGTACACATGGAACATTCAGCATGATCATATCTTTGCTCATAAACACACCTGATTTAAATTGACAAACTTGGATCTAGTATGATAAACTGATG
GCTATAGACAAAATCAGCACCTTATTTATCCCCAAAGATGTTAAGTCACTTAACCTTGATGTGAATACGGTGGCCAAATTAGAAGCGGACAATTAGAATA
TAAAAGAAAATGCTCAAGAATTAAATGGTGGCCAGGCACAGTAACTCCTGCCTGCAATCCCAGTACTTTGGGAGGCTCGCTTGAGCTCAGGGTTTTGAGT
CCAGCCTGGGCAACACACAGGGAGACCCTGTCTCCACAAAATGTTAGCCAGACATGGTGGTGTGCACCTGTAGTCCTCGCTACTTGGAAGGCTTAGGTGG
CAGGATGGATTGAGCCCAGGAGGTTGAAACTGCAGTGAGCTGATTGTGCCCAGCCTGGGCAACAAAGCAAGACCCCATCTCAAGAAAAATAAATTAAAAA
AAACTAAA
  • refseq_DCLRE1C.F3 (forward primer)
  • refseq_DCLRE1C.R3 (reverse primer)
  • Single exon skipping

Frame 0

ASRKWRRGQGWPWLQLRFWGPGLWDRRRYEFFR
GADGRVSNYLHRPLR*GEPEGPRLLPVPLPQRSH
ERIKSPYLEKKVGVQMRKLRNQRG*ISCPVSHS
Q*VAGPGFQPA*RFIYTVHL*LRSCC*RARNTD
FGRNELAVTTAGLFLLSLAFSLKLLQVSPASSQE
GKLEEKWRGTETNWKAEWPTRTDRNPLLSLTSS
GLNSAGLPLWL*STCTCPRTRRSCTRAPSRS*C
CCGPAPDSELSQKISIEIETPTQISLVDEASGEK
EEIVVTLLPAGHCPGSVMFLFQGNNGTVLYTGD
FRLAQGEAARMELLHSGGRVKDIQSVYLDTTFC
DPRFYQIPSREECLSGVLELVRSWITRSPYHVVW
LNCKAAYGYEYLFTNLSEELGVQVHVNKLDMFR
NMPEILHHLTTDRNTQIHACRHPKAEEYFQWSK
LPCGITSRNRIPLHIISIKPSTMWFGERSRKTNV
IVRTGESSYRACFSFHSSYSEIKDFLSYLCPVN
AYPNVIPVGTTMDKVVEILKPLCRSSQSTEPKY
KPLGKLKRARTVHRDSEEEDDYLFDDPLPIPLRH
KVPYPETFHPEVFSMTAVSEKQPEKLRQTPGCC
RAECMQSSRFTNFVDCEESNSESEEEVGIPASL
QGDLGSVLHLQKADGDVPQWEVFFKRNDEITDES
LENFPSSTVAGGSQSPKLFSDSDGESTHISSQN
SSQSTHITEQGSQGWDSQSDTVLLSSQERNSGD
ITSLDKADYRPTIKENIPASLMEQNVICPKDTYS
DLKSRDKDVTIVPSTGEPTTLSSETHIPEEKSL
LNLSTNADSQSSSDFEVPSTPEAELPKREHLQY
LYEKLATGESIAVKKRKCSLLDT*EFKAFQPRAT
TKKPAQR*QSILQ*RKYST*KCSNNLVGCGGSH
L*SQHFEVGNGLSPGVRHQPGQHSEMCLYLQKK
KKKKKPGMVVGYSGGP*GSLEPRRLRLQ*AMTVP
LHL*SG*QSKTLFKKEKGQARWLMPVIPATQEA
KAGESLEPGRWWLQ*AEIVSLHSSLGNKSKTLS
QKKKKKKRKKNGRTKPIRINDYIKCKWSKCINDK
RHGKNQHNPVICCL*RKSIQVIDRLKGWKNHVN
N*KWSGYMDSFLMIKGLVHQEDKIILNVYAPNS
SKNL*S*NS*KETN*CTITARD*FNIPP**QN*Y
IHINQ*EYRTENHKPTGLN*HL*NTPPQTSRMY
LLKYTWNIQHDHIFAHKHT*FKLTNLDLV**TD
GYRQNQHLIYPQRC*VT*P*CEYGGQIRSGQLEY
KRKCSRIKWWPGTVTPACNPSTLGGSLELRVLS
PAWATHRETLSPQNVSQTWWCAPVVLATWKA*V
AGWIEPRRLKLQ*ADCAQPGQQSKTPSQEK*IKK
N*

Frame 1

LPGSGGAVRAGLGFSCGFGVPDSGIGGAMSSFE
GQMAEYPTISIDRFDRENLRARAYFLSHCHKDH
MKGLRAPTLKRRLECR*GN*GTREVKLVAQCHTA
SKWQDQDSSQLEGLSILFTCD*GVVVNEPEIQI
LEETN*QSQQQGCSCCLWHSA*SYYRSARPAVR
KESWKRSGGEQRQTGRQNGPLGQTETHFCLSLPL
ASIVLVSRFGSEAHAPAPGLGGAAHGHPAEADV
AAALPQTLS*ARRYLLKSRLLPRYL*WMKHQER
RKRLL*LSYQLVTVRDQLCFYFRAIMELSCTQET
SDWRKEKLLEWSFCTPGAESKTSKVYIWILRSV
IQDFTKFQVGRSV*VES*SWSEAGSLGARTMLC
G*TAKRLMAMNICSPTLVKN*ESRFM*IS*TCLG
TCLRSFIISQQTATLRSMHAGIPRQRNIFSGAN
YPVELLPEIEFHST*SALSHPPCGLEKGAEKQM
*L*GLERVHTELVFLFTPPTVRLKIS*ATSVL*T
HIQMSFQLAQLWIKLSKS*SLYAGLPKVRSQSI
NHWEN*RELEQFTETQRRKMTISLMILCQYL*G
TKFHTRKLFTLRYFQ*LQYQKSSLKN*DKPQDAA
EQSVCRALVSQTL*IVKNPTVKVKKK*ESQLHC
KEIWALYFTCKRLMGMYPSGKYSLKEMMKSQMR
VWKTSLPPQWQGDLSHQSFSVTLMENQLTSPPRI
LPSQHT*QNKEVKAGTANLILFCYLPKRETVGI
LLPWTKLTTDQQSKRIFLPLSWNKM*FAQRILT
LI*KAEIKM*Q*FLVLENQLL*AVRHIYPRKKVC
*ILAQMQIPRALLILKFPQLQKLSYLNESIYNI
YMRSWQLVRV*QSKKENAHS*IPKNSKRFNLEQ
PLKNLHRDDSQYYNRENTVLKNVQITWLGVVAHT
CNPSTLRWAMA*AQEFDTSLANTVKCVSTYKKK
KKKKSQAWWWATLEALEDHLSPGD*GCSEP*LC
HCTSSLGDRARPCLKKKKARHGGSCL*SQLRRRP
RQENHLNLGGGGCSELRLCHCTPAWATRAKLCL
KKKKKKKEKKMVELNPYVSMITLNVNGLSASMT
RDTEKTNITQ*SAVYKESQFK*LIG*KDGKTM*T
IKNGVVIWTVS***KV*FTKKTK*S*TYMHLTA
PKIYEARTAEKRQTNAQLQLGINLTSLLSNRTS
IYTLISKNIELKIISRLD*IDIYRTHHPKLAECI
S*STHGTFSMIISLLINTPDLN*QTWI*YDKLM
AIDKISTLFIPKDVKSLNLDVNTVAKLEADN*N
IKENAQELNGGQAQ*LLPAIPVLWEARLSSGF*V
QPGQHTGRPCLHKMLARHGGVHL*SSLLGRLRW
QDGLSPGG*NCSELIVPSLGNKARPHLKKNKLK
KTK

Frame 2

FPEVAARSGLALASAAVLGSRTLGSAAL*VLSR
GRWPSIQLSP*TASIGRT*GPAPTSCPTATKIT
*KD*EPLP*KEGWSADEETEEPERLN*LPSVTQ
PVSGRTRIPASLKVYLYCSPVTKELLLTSPKYRF
WKKRISSHNSRVVPVVSGIQLEVTTGQPGQQSG
RKAGREVEGNRDKLEGRMAH*DRPKPTSVSHFL
WPQ*CWSPALALKHMHLPQDSEELHTGTQQKLML
LRPCPRL*VKPEDIY*NRDSYPDIFSG*SIRRE
GRDCCDSLTSWSLSGISYVFISGQ*WNCPVHRR
LQIGARRSC*NGASALRGQSQRHPKCIFGYYVL*
SKILPNSKSGGVFKWSLRAGPKLDHSEPVPCCV
AELQSGLWL*ISVHQP**RIRSPGSCE*ARHV*
EHA*DPSSSHNRPQHSDPCMPASQGRGIFSVEQI
TLWNYFQK*NSTPHNQH*AIHHVVWRKEQKNKC
NCEDWREFIQSLFFFSLLLQ*D*RFLELPLSCE
RISKCHSSWHNYG*SCRNLKAFMPVFPKYGAKV*
TTGKTEES*NSSPRLRGGR*LSL**SSANTFKA
QSSIPGNFSP*GIFNDCSIRKAA*KTETNPRML
QSRVYAELSFHKLCRL*RIQQ*K*RRSRNPSFTA
RRSGLCTSPAKG*WGCTPVGSIL*KK**NHR*E
FGKLPFLHSGRGISVTKAFQ*L*WRINSHLLPE
FFPVNTHNRTRKSRLGQPI*YCFVIFPREKQWGY
YFLGQS*LQTNNQREYSCLSHGTKCNLPKGYLL
*FEKQR*RCDNSS*YWRTNYSKQ*DTYTRGKKF
AKS*HKCRFPELF*F*SSLNSRS*VT*TRAFTIF
I*EAGNW*EYSSQKKKMLTLRYLRIQSVST*SN
H*KTCTEMTVNITIEKIQYLKMFK*PGWVWWLT
LVIPAL*GGQWLEPRSSTPAWPTQ*NVSLLTKKK
KKKKARHGGGLLWRPLRIT*AQEIEAAVSHDCA
TAPLVWVTEQDPV*KRKRPGTVAHACNPSYAGG
QGRRIT*TWEVVVAVS*DCVIALQPGQQEQNSVS
KKKKKKKKKKW*N*THTYQ*LH*M*MV*VHQ*Q
ETRKKPT*PSDLLSIKKVNSSD**VKRMEKPCK
QLKMEWLYGQFPNDKRSSSPRRQNNPKRICT*QL
QKFMKLEQLKRDKLMHNYS*GLI*HPSLVTELV
YTH*SVRI*N*KS*ADWIKLTFIEHTTPN*QNV
SPEVHMEHSA*SYLCS*THLI*IDKLGSSMIN*W
L*TKSAPYLSPKMLSHLTLM*IRWPN*KRTIRI
*KKMLKN*MVARHSNSCLQSQYFGRLA*AQGFE
SSLGNTQGDPVSTKC*PDMVVCTCSPRYLEGLGG
RMD*AQEVETAVS*LCPAWATKQDPISRKIN*K
KL

Sequence NM_001033857

GCTTCCCGGAAGTGGCGGCGCGGTCAGGGCTGGCCTTGGCTTCAGCTGCGGTTTTGGGGTCCCGGACTCTGGGATCGGCGGCGCTATGAGTTCTTTCGAG
GGGCAGATGGCCGAGTATCCAACTATCTCCATAGACCGCTTCGATAGGGAGAACCTGAGGGCCCGCGCCTACTTCCTGTCCCACTGCCACAAAGATCACA
TGAAAGGATTAAGAGCCCCTACCTTGAAAAGAAGGTTGGAGTGCAGATGAGGAAACTGAGGAACCAGAGAGGTTAAATTAGTTGCCCAGTGTCACACAGC
CAGTAAGTGGCAGGACCAGGATTCCAGCCAGCTTGAAGGTTTATCTATACTGTTCACCTGTGACTAAGGAGTTGTTGTTAACGAGCCCGAAATACAGATT
TTGGAAGAAACGAATTATATCTATTGAAATCGAGACTCCTACCCAGATATCTTTAGTGGATGAAGCATCAGGAGAGAAGGAAGAGATTGTTGTGACTCTC
TTACCAGCTGGTCACTGTCCGGGATCAGTTATGTTTTTATTTCAGGGCAATAATGGAACTGTCCTGTACACAGGAGACTTCAGATTGGCGCAAGGAGAAG
CTGCTAGAATGGAGCTTCTGCACTCCGGGGGCAGAGTCAAAGACATCCAAAGTGTATATTTGGATACTACGTTCTGTGATCCAAGATTTTACCAAATTCC
AAGTCGGGAGGAGTGTTTAAGTGGAGTCTTAGAGCTGGTCCGAAGCTGGATCACTCGGAGCCCGTACCATGTTGTGTGGCTGAACTGCAAAGCGGCTTAT
GGCTATGAATATCTGTTCACCAACCTTAGTGAAGAATTAGGAGTCCAGGTTCATGTGAATAAGCTAGACATGTTTAGGAACATGCCTGAGATCCTTCATC
ATCTCACAACAGACCGCAACACTCAGATCCATGCATGCCGGCATCCCAAGGCAGAGGAATATTTTCAGTGGAGCAAATTACCCTGTGGAATTACTTCCAG
AAATAGAATTCCACTCCACATAATCAGCATTAAGCCATCCACCATGTGGTTTGGAGAAAGGAGCAGAAAAACAAATGTAATTGTGAGGACTGGAGAGAGT
TCATACAGAGCTTGTTTTTCTTTTCACTCCTCCTACAGTGAGATTAAAGATTTCTTGAGCTACCTCTGTCCTGTGAACGCATATCCAAATGTCATTCCAG
TTGGCACAACTATGGATAAAGTTGTCGAAATCTTAAAGCCTTTATGCCGGTCTTCCCAAAGTACGGAGCCAAAGTATAAACCACTGGGAAAACTGAAGAG
AGCTAGAACAGTTCACCGAGACTCAGAGGAGGAAGATGACTATCTCTTTGATGATCCTCTGCCAATACCTTTAAGGCACAAAGTTCCATACCCGGAAACT
TTTCACCCTGAGGTATTTTCAATGACTGCAGTATCAGAAAAGCAGCCTGAAAAACTGAGACAAACCCCAGGATGCTGCAGAGCAGAGTGTATGCAGAGCT
CTCGTTTCACAAACTTTGTAGATTGTGAAGAATCCAACAGTGAAAGTGAAGAAGAAGTAGGAATCCCAGCTTCACTGCAAGGAGATCTGGGCTCTGTACT
TCACCTGCAAAAGGCTGATGGGGATGTACCCCAGTGGGAAGTATTCTTTAAAAGAAATGATGAAATCACAGATGAGAGTTTGGAAAACTTCCCTTCCTCC
ACAGTGGCAGGGGGATCTCAGTCACCAAAGCTTTTCAGTGACTCTGATGGAGAATCAACTCACATCTCCTCCCAGAATTCTTCCCAGTCAACACACATAA
CAGAACAAGGAAGTCAAGGCTGGGACAGCCAATCTGATACTGTTTTGTTATCTTCCCAAGAGAGAAACAGTGGGGATATTACTTCCTTGGACAAAGCTGA
CTACAGACCAACAATCAAAGAGAATATTCCTGCCTCTCTCATGGAACAAAATGTAATTTGCCCAAAGGATACTTACTCTGATTTGAAAAGCAGAGATAAA
GATGTGACAATAGTTCCTAGTACTGGAGAACCAACTACTCTAAGCAGTGAGACACATATACCCGAGGAAAAAAGTTTGCTAAATCTTAGCACAAATGCAG
ATTCCCAGAGCTCTTCTGATTTTGAAGTTCCCTCAACTCCAGAAGCTGAGTTACCTAAACGAGAGCATTTACAATATTTATATGAGAAGCTGGCAACTGG
TGAGAGTATAGCAGTCAAAAAAAGAAAATGCTCACTCTTAGATACCTAAGAATTCAAAGCGTTTCAACCTAGAGCAACCACTAAAAAACCTGCACAGAGA
TGACAGTCAATATTACAATAGAGAAAATACAGTACTTAAAAATGTTCAAATAACCTGGTTGGGTGTGGTGGCTCACACTTGTAATCCCAGCACTTTGAGG
TGGGCAATGGCTTGAGCCCAGGAGTTCGACACCAGCCTGGCCAACACAGTGAAATGTGTCTCTACTTACAAAAAAAAAAAAAAAAAAAAAAGCCAGGCAT
GGTGGTGGGCTACTCTGGAGGCCCTTGAGGATCACTTGAGCCCAGGAGATTGAGGCTGCAGTGAGCCATGACTGTGCCACTGCACCTCTAGTCTGGGTGA
CAGAGCAAGACCCTGTTTAAAAAAGAAAAAGGCCAGGCACGGTGGCTCATGCCTGTAATCCCAGCTACGCAGGAGGCCAAGGCAGGAGAATCACTTGAAC
CTGGGAGGTGGTGGTTGCAGTGAGCTGAGATTGTGTCATTGCACTCCAGCCTGGGCAACAAGAGCAAAACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAG
AAAAAAAAATGGTAGAACTAAACCCATACGTATCAATGATTACATTAAATGTAAATGGTCTAAGTGCATCAATGACAAGAGACACGGAAAAAACCAACAT
AACCCAGTGATCTGCTGTCTATAAAGAAAGTCAATTCAAGTGATTGATAGGTTAAAAGGATGGAAAAACCATGTAAACAATTAAAAATGGAGTGGTTATA
TGGACAGTTTCCTAATGATAAAAGGTCTAGTTCACCAAGAAGACAAAATAATCCTAAACGTATATGCACCTAACAGCTCCAAAAATTTATGAAGCTAGAA
CAGCTGAAAAGAGACAAACTAATGCACAATTACAGCTAGGGATTAATTTAACATCCCTCCTTAGTAACAGAACTAGTATATACACATTAATCAGTAAGAA
TATAGAACTGAAAATCATAAGCCGACTGGATTAAATTGACATTTATAGAACACACCACCCCAAACTAGCAGAATGTATCTCCTGAAGTACACATGGAACA
TTCAGCATGATCATATCTTTGCTCATAAACACACCTGATTTAAATTGACAAACTTGGATCTAGTATGATAAACTGATGGCTATAGACAAAATCAGCACCT
TATTTATCCCCAAAGATGTTAAGTCACTTAACCTTGATGTGAATACGGTGGCCAAATTAGAAGCGGACAATTAGAATATAAAAGAAAATGCTCAAGAATT
AAATGGTGGCCAGGCACAGTAACTCCTGCCTGCAATCCCAGTACTTTGGGAGGCTCGCTTGAGCTCAGGGTTTTGAGTCCAGCCTGGGCAACACACAGGG
AGACCCTGTCTCCACAAAATGTTAGCCAGACATGGTGGTGTGCACCTGTAGTCCTCGCTACTTGGAAGGCTTAGGTGGCAGGATGGATTGAGCCCAGGAG
GTTGAAACTGCAGTGAGCTGATTGTGCCCAGCCTGGGCAACAAAGCAAGACCCCATCTCAAGAAAAATAAATTAAAAAAAACTAAA
  • refseq_DCLRE1C.F3 (forward primer)
  • refseq_DCLRE1C.R3 (reverse primer)
  • Single exon skipping

Frame 0

ASRKWRRGQGWPWLQLRFWGPGLWDRRRYEFFR
GADGRVSNYLHRPLR*GEPEGPRLLPVPLPQRSH
ERIKSPYLEKKVGVQMRKLRNQRG*ISCPVSHS
Q*VAGPGFQPA*RFIYTVHL*LRSCC*RARNTD
FGRNELYLLKSRLLPRYL*WMKHQERRKRLL*LS
YQLVTVRDQLCFYFRAIMELSCTQETSDWRKEK
LLEWSFCTPGAESKTSKVYIWILRSVIQDFTKF
QVGRSV*VES*SWSEAGSLGARTMLCG*TAKRLM
AMNICSPTLVKN*ESRFM*IS*TCLGTCLRSFI
ISQQTATLRSMHAGIPRQRNIFSGANYPVELLP
EIEFHST*SALSHPPCGLEKGAEKQM*L*GLERV
HTELVFLFTPPTVRLKIS*ATSVL*THIQMSFQ
LAQLWIKLSKS*SLYAGLPKVRSQSINHWEN*R
ELEQFTETQRRKMTISLMILCQYL*GTKFHTRKL
FTLRYFQ*LQYQKSSLKN*DKPQDAAEQSVCRA
LVSQTL*IVKNPTVKVKKK*ESQLHCKEIWALY
FTCKRLMGMYPSGKYSLKEMMKSQMRVWKTSLPP
QWQGDLSHQSFSVTLMENQLTSPPRILPSQHT*
QNKEVKAGTANLILFCYLPKRETVGILLPWTKL
TTDQQSKRIFLPLSWNKM*FAQRILTLI*KAEIK
M*Q*FLVLENQLL*AVRHIYPRKKVC*ILAQMQ
IPRALLILKFPQLQKLSYLNESIYNIYMRSWQL
VRV*QSKKENAHS*IPKNSKRFNLEQPLKNLHRD
DSQYYNRENTVLKNVQITWLGVVAHTCNPSTLR
WAMA*AQEFDTSLANTVKCVSTYKKKKKKKSQA
WWWATLEALEDHLSPGD*GCSEP*LCHCTSSLGD
RARPCLKKKKARHGGSCL*SQLRRRPRQENHLN
LGGGGCSELRLCHCTPAWATRAKLCLKKKKKKK
EKKMVELNPYVSMITLNVNGLSASMTRDTEKTNI
TQ*SAVYKESQFK*LIG*KDGKTM*TIKNGVVI
WTVS***KV*FTKKTK*S*TYMHLTAPKIYEAR
TAEKRQTNAQLQLGINLTSLLSNRTSIYTLISKN
IELKIISRLD*IDIYRTHHPKLAECIS*STHGT
FSMIISLLINTPDLN*QTWI*YDKLMAIDKIST
LFIPKDVKSLNLDVNTVAKLEADN*NIKENAQEL
NGGQAQ*LLPAIPVLWEARLSSGF*VQPGQHTG
RPCLHKMLARHGGVHL*SSLLGRLRWQDGLSPG
G*NCSELIVPSLGNKARPHLKKNKLKKTK

Frame 1

LPGSGGAVRAGLGFSCGFGVPDSGIGGAMSSFE
GQMAEYPTISIDRFDRENLRARAYFLSHCHKDH
MKGLRAPTLKRRLECR*GN*GTREVKLVAQCHTA
SKWQDQDSSQLEGLSILFTCD*GVVVNEPEIQI
LEETNYIY*NRDSYPDIFSG*SIRREGRDCCDS
LTSWSLSGISYVFISGQ*WNCPVHRRLQIGARRS
C*NGASALRGQSQRHPKCIFGYYVL*SKILPNS
KSGGVFKWSLRAGPKLDHSEPVPCCVAELQSGL
WL*ISVHQP**RIRSPGSCE*ARHV*EHA*DPSS
SHNRPQHSDPCMPASQGRGIFSVEQITLWNYFQ
K*NSTPHNQH*AIHHVVWRKEQKNKCNCEDWRE
FIQSLFFFSLLLQ*D*RFLELPLSCERISKCHSS
WHNYG*SCRNLKAFMPVFPKYGAKV*TTGKTEE
S*NSSPRLRGGR*LSL**SSANTFKAQSSIPGN
FSP*GIFNDCSIRKAA*KTETNPRMLQSRVYAEL
SFHKLCRL*RIQQ*K*RRSRNPSFTARRSGLCT
SPAKG*WGCTPVGSIL*KK**NHR*EFGKLPFL
HSGRGISVTKAFQ*L*WRINSHLLPEFFPVNTHN
RTRKSRLGQPI*YCFVIFPREKQWGYYFLGQS*
LQTNNQREYSCLSHGTKCNLPKGYLL*FEKQR*
RCDNSS*YWRTNYSKQ*DTYTRGKKFAKS*HKCR
FPELF*F*SSLNSRS*VT*TRAFTIFI*EAGNW
*EYSSQKKKMLTLRYLRIQSVST*SNH*KTCTE
MTVNITIEKIQYLKMFK*PGWVWWLTLVIPAL*G
GQWLEPRSSTPAWPTQ*NVSLLTKKKKKKKARH
GGGLLWRPLRIT*AQEIEAAVSHDCATAPLVWV
TEQDPV*KRKRPGTVAHACNPSYAGGQGRRIT*T
WEVVVAVS*DCVIALQPGQQEQNSVSKKKKKKK
KKKW*N*THTYQ*LH*M*MV*VHQ*QETRKKPT
*PSDLLSIKKVNSSD**VKRMEKPCKQLKMEWLY
GQFPNDKRSSSPRRQNNPKRICT*QLQKFMKLE
QLKRDKLMHNYS*GLI*HPSLVTELVYTH*SVR
I*N*KS*ADWIKLTFIEHTTPN*QNVSPEVHMEH
SA*SYLCS*THLI*IDKLGSSMIN*WL*TKSAP
YLSPKMLSHLTLM*IRWPN*KRTIRI*KKMLKN
*MVARHSNSCLQSQYFGRLA*AQGFESSLGNTQG
DPVSTKC*PDMVVCTCSPRYLEGLGGRMD*AQE
VETAVS*LCPAWATKQDPISRKIN*KKL

Frame 2

FPEVAARSGLALASAAVLGSRTLGSAAL*VLSR
GRWPSIQLSP*TASIGRT*GPAPTSCPTATKIT
*KD*EPLP*KEGWSADEETEEPERLN*LPSVTQ
PVSGRTRIPASLKVYLYCSPVTKELLLTSPKYRF
WKKRIISIEIETPTQISLVDEASGEKEEIVVTL
LPAGHCPGSVMFLFQGNNGTVLYTGDFRLAQGE
AARMELLHSGGRVKDIQSVYLDTTFCDPRFYQIP
SREECLSGVLELVRSWITRSPYHVVWLNCKAAY
GYEYLFTNLSEELGVQVHVNKLDMFRNMPEILH
HLTTDRNTQIHACRHPKAEEYFQWSKLPCGITSR
NRIPLHIISIKPSTMWFGERSRKTNVIVRTGES
SYRACFSFHSSYSEIKDFLSYLCPVNAYPNVIP
VGTTMDKVVEILKPLCRSSQSTEPKYKPLGKLKR
ARTVHRDSEEEDDYLFDDPLPIPLRHKVPYPET
FHPEVFSMTAVSEKQPEKLRQTPGCCRAECMQS
SRFTNFVDCEESNSESEEEVGIPASLQGDLGSVL
HLQKADGDVPQWEVFFKRNDEITDESLENFPSS
TVAGGSQSPKLFSDSDGESTHISSQNSSQSTHI
TEQGSQGWDSQSDTVLLSSQERNSGDITSLDKAD
YRPTIKENIPASLMEQNVICPKDTYSDLKSRDK
DVTIVPSTGEPTTLSSETHIPEEKSLLNLSTNA
DSQSSSDFEVPSTPEAELPKREHLQYLYEKLATG
ESIAVKKRKCSLLDT*EFKAFQPRATTKKPAQR
*QSILQ*RKYST*KCSNNLVGCGGSHL*SQHFE
VGNGLSPGVRHQPGQHSEMCLYLQKKKKKKKPGM
VVGYSGGP*GSLEPRRLRLQ*AMTVPLHL*SG*
QSKTLFKKEKGQARWLMPVIPATQEAKAGESLE
PGRWWLQ*AEIVSLHSSLGNKSKTLSQKKKKKKR
KKNGRTKPIRINDYIKCKWSKCINDKRHGKNQH
NPVICCL*RKSIQVIDRLKGWKNHVNN*KWSGY
MDSFLMIKGLVHQEDKIILNVYAPNSSKNL*S*N
S*KETN*CTITARD*FNIPP**QN*YIHINQ*E
YRTENHKPTGLN*HL*NTPPQTSRMYLLKYTWN
IQHDHIFAHKHT*FKLTNLDLV**TDGYRQNQHL
IYPQRC*VT*P*CEYGGQIRSGQLEYKRKCSRI
KWWPGTVTPACNPSTLGGSLELRVLSPAWATHR
ETLSPQNVSQTWWCAPVVLATWKA*VAGWIEPRR
LKLQ*ADCAQPGQQSKTPSQEK*IKKN*

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