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Sequences

Sequence NM_170719

ACTTGGGCGCGACTGGCTTTATTGTAGGTCCCCATGAGCTGGTGGGGGCTGTAGGGGTAACCAGGCAGGGCGTCTGGCGCTGTTGGGGCTGCGTTTTTCC
GGAGTCCCCCTATGCGAGGCCGTGCCCCTCTTCGCCCGGCTCCTGGTGCTCCCCAGCCGCCGGCCCGCTCCCGGAAGCTGCAGCAGCTGGTAACAAAGAG
CCTGCCGGGCCGCCTGCTGCCGGGGCCGAGGTATCCAGGAGTCGGGCGGAGCGGCTGGGGATGAGCGCGAGCCGGACCATGCGTCCGGGTTCGCGGCGGT
GAGGGTCGCGGCCGGCGGCCGAGGCGCAGGTGCGGGTCGAGAGACCGGCGCCTCCCTCCCCGCCGCCCGTGGACTCTGAGCGCAGGTGTTTCTACTCTGC
CGGCCGCTCCACCTGGGAAAGCGCCTTCAAGAGCTACCTCTGGCGGCACCTGGCCCTCAGCTGCAGGGAAACCGCACTCAAACCTGCCGCCGAGCCCGGG
TTTTTCTGCAAAACTCAGAGGACCTGGCTCTTGCCCGAGGTGCCCTGAACCATTGTGTGAATAAGCTGGGGAAGCCTGACTGGAGTGTTTTTTCTTTTTA
ATAAAAAGAGTTTTTCTGAATCTCAATTTTTAGAAATTTTTTTTGCGAAGTTTGCAATACTCTCTACCTCTTCGCATCCTCTCTTGCTCGTGGAGTCTTT
TTCCTCCTCAGCTATAAATATTTTTTACTATCGGCGGAGCAGACCAGGGATGAACGTCTTTTAATTGCAAGTATAACTGTTAAAACCACGTCGGGATAGT
AGTCAAGATGGATAAAAAGTCCTTTGAAATGGTGCTGGATGAAATTAGAAAGGTGGTTGATTTGCTGAGATATTTTTCCTGGGCCGAGCCCCAGTTGAAG
GCAATGAAAGCATTACAGCATAAAATGGTGGCTGTCCAGCCAACAGAAGTGGTCAATATACTCAACTGTTTCACTTTCAGTAAAGACAAACTAGTTGCTC
TTGAACTGTTAGCCTCGAACATTATTGATGCACAGAATTCTCGTCCTATTGAAGATTTATTCAGGGTAAATATGTCTGAGAAGAAACGGTGCAAGAGAAT
ACTTGAACAGGCTTTCAAGGGGGGCTGCAAAGCTCCTCATGCTATGATATCTTCTTGTGGAACAATCCCAGGAAATCCATATCCCAAAGGAAGACCTAGC
CGCATAAATGGAATTTTCCCAGGAACTCCTTTGAAAAAAGATGGTGAAGAATGTACTAACGAAGGCAAAGGAATAGCTGCACGAATTCTTGGGCCATCCA
AACCACCTCCTTCAACATATAATCCACATAAACCTGTTCCTTATCCGATACCTCCATGCCGACCACATGCAACTATTGCACCAAGTGCTTATAACAATGC
AGGTCTGGTACCATTAGCGAATGTCATAGCTCCACCTCCACCTCCATATACTCCTAATCCTGTAGGAACAGAGAATGAAGACCTTTCGAATCCGTCAAAA
CCTATACAGAATCAAACATTTTCCACCCCAGCAAGTCAACTCTTTTCTCCTCATGGTTCTAATCCTTCAACACCTGCTGCAACTCCTGTTCCTACTGCAT
CCCCAGTCAAGGCAATTAATCATCCATCAGCATCAGCAGCTGCCACCGTTTCTGGAATGAACCTGCTGAATACTGTCCTTCCTGTGTTCCCAGGGCAGGT
CTCCTCAGCCGTTCACACACCTCAGCCATCAATACCAAACCCAACAGTTATCAGAACCCCTTCATTGCCCACTGCACCTGTTACATCCATCCACAGTACA
ACCACCACTCCTGTTCCTTCCATTTTTTCTGGCCTAGTGTCACTGCCAGGTCCTTCTGCCACTCCTACCGCAGCCACTCCTACCCCAGGACCTACACCAC
GGTCCACTCTTGGTTCCAGTGAAGCATTTGCTTCTACTTCTGCACCTTTCACTAGCCTCCCCTTTTCCACCAGCTCTTCTGCTGCTTCTACCAGCAACCC
AAATTCTGCTTCATTGTCATCAGTTTTTGCAGGGCTCCCTTTGCCCTTACCACCAACATCCCAAGGCCTATCCAACCCGACTCCTGTAATTGCTGGTGGC
TCTACTCCCAGCGTTGCCGGTCCACTTGGTGTGAACAGTCCTCTTTTGTCTGCGTTAAAAGGTTTTCTGACATCCAATGACACCAATTTAATCAACTCCT
CTGCTTTATCCTCTGCTGTCACAAGTGGGCTGGCTTCACTATCTTCTCTTACTCTTCAGAACTCTGACTCTTCTGCTTCAGCCCCTAACAAGTGCTATGC
CCCATCAGCCATCCCTACCCCACAGAGGACTTCCACTCCAGGGTTGGCCCTGTTCCCAGGCCTGCCGTCTCCCGTGGCTAACTCAACTTCCACTCCCCTG
ACATTGCCTGTACAGTCTCCTTTAGCCACTGCTGCATCAGCTTCCACGTCAGTGCCAGTTAGCTGTGGCTCCTCAGCCTCCCTTTTGCGTGGCCCCCACC
CAGGTACCTCAGATCTGCATATTTCATCTACCCCTGCTGCAACAACTCTTCCTGTTATGATCAAAACTGAGCCCACAAGTCCTACTCCCTCGGCCTTCAA
AGGTCCATCTCATTCTGGGAATCCCTCTCATGGCACTTTAGGTTTGTCAGGGACATTGGGCCGTGCATATACTTCAACATCCGTGCCCATCAGTTTATCT
GCTTGCCTTAATCCTGCATTGTCAGGTCTCTCCAGCTTGAGTACTCCTTTAAATGGTTCAAATCCTCTTTCCTCTATTTCCCTTCCACCACATGGTTCCT
CCACTCCCATTGCACCAGTATTCACTGCTCTTCCTTCTTTTACTTCTTTGACCAACAATTTTCCTTTAACTGGCAACCCATCTCTTAATCCGTCAGTATC
TCTCCCAGGGTCATTAATAGCCACCTCATCTACCGCTGCCACCTCCACATCTCTCCCTCATCCTAGCTCAACGGCAGCTGTTCTCTCAGGGCTTTCTGCT
TCAGCACCAGTCTCAGCAGCACCTTTCCCCCTCAACCTGTCCACTGCTGTTCCCTCACTTTTCTCTGTTACTCAAGGACCTCTGTCATCTTCAAATCCCT
CCTATCCAGGCTTTTCTGTCTCTAATACCCCAAGCGTTACCCCTGCTCTTCCCTCATTCCCGGGGCTGCAGGCGCCCTCTACAGTCGCAGCTGTCACACC
ACTACCTGTGGCTGCCACAGCCCCATCCCCAGCTCCAGTCCTCCCAGGATTCGCCTCAGCATTCAGTTCCAATTTCAACTCCGCTCTTGTTGCACAAGCC
GGTTTATCATCTGGACTTCAAGCTGCAGGCAGTTCTGTTTTTCCAGGCCTTTTGTCCCTCCCGGGTATCCCTGGGTTTCCTCAGAATCCTTCACAATCAT
CCTTGCAAGAATTACAGCATAATGCGGCTGCGCAGTCAGCATTGTTACAGCAGGTCCATTCAGCTTCGGCTCTGGAAAGCTATCCAGCTCAGCCTGATGG
GTTTCCTAGTTATCCTTCAGCGCCAGGAACACCATTTTCTTTGCAACCAAGCCTGTCCCAGAGTGGGTGGCAGTGAATACTTTTAACTTTTATTCTCCTT
CAGAGCAACATCAGAATTGCCTGAGAACTGCAATGAACAATCTGACAAATGTGAAGCTGGCCAAAAGTCGGAAAATGAGAATGAGGGTAATCCTGGAGAA
ATTGTGACAACAATTTGAAAATTGTGGTTGCATTTTAAAGTGTGAACACTCCCCTATGTAAATATGCTGACAATAAATTGTATGGAGAATGGTATTTAAA
AAGTGTTTGGAGACTTTTCACCTGTCCTATAAAATTTTGAATTGTGTATGTGATCTACATAGAAAGAATATTAAAGAGTAGGTTGAACTCTTTATAGCCG
AATACAGCCTTAAATATGCTTGTATAGCATCCACTGGCAGAAGTAATAGTTGTGCCTCAGACTTGGGGGTTGCATGTGGCCCTGGGGGAGTTACTACCCT
TGGTATGCATGAGCGGTTCCTATTAGCATCAGTGGGAACTCAGTACTCTGTATGTATCCACAAAAGGGAACTTGAGACCCACAGTTATTCTTAATTTCTG
ATATTAACAACCGTACATACTGCTGAATTTAACTCAAAATATTTCAGGTAAGTGAAAGTGGTGCTTAATGTAGACTATAGAATGACTTTCAGGTGTTTTC
AACTGAAAGTATATATCCAGAACTGCATCCTTATAGAAATACAAGTAAGACTTAGGATAATTTGCCTTCAAAACAGTTTTCCTAATCTCAGCAGTATCCA
GTGAGTGAAGAACACTTGACTGACTCTTGGGCCACCTCTGTTACTTACTGTACTATGGAAGCTCCTGGTGAATGTTTACAATTATGGGATGTAGTATTTC
TATTTGTACTTTAAGTCAAATGCTTATATGAAATATGTGACAACAAATAGAGAAGACTGGCTCTGTTAGTAATTATGCAGTATGTACTCTATTTAAGGAT
CTGTGGTAGTATAACATGAGTGAATGTCATTAATTTTGAAGTAATAACTGCCACATGTGGGAAGTAGGGGAGTAAGGAGAATGAATTCCAATCTGTGATT
AAAAGTGTAAACTATAGACTCTACTGTAGTACATTTCAGGATCTAGAAGTTTTACTTTTATAAAGATGGTGTCCGGAAGATGTTGCTAATGTATTTTACT
TCAACATAGGGAACAAACTTTTTAAGTATATTAATAAACCTGTATGGTTAGTTTTTAACAGTTTTTTAAAATAAACTATGGATATGACAAATATTCTGTG
TTTTACTAAGTGCTTGGATAGGCTTTCTAATTTTGTATACGTGCTAGAGTTAATTATTGAACATTTTTATCCAAATTTAGTTGTAACTCTGTTTATACTA
CTGATTGCTCATTCGTTTAAATGATATTTTAATGTAAAAGTCATAACCAACATATGAACAGACAGATTTATGTCTTTAAACACAGAATGTAAGCTATAGT
TTAATCTGATACCAGTTGCTGGAAGTTGCCATTTGTTTTTCTTAAATCTATACCCATAAAACTTCTTTTAAGATTA
  • refseq_C13orf23.F2 (forward primer)
  • refseq_C13orf23.R2 (reverse primer)
  • Single exon skipping

Frame 0

TWARLALL*VPMSWWGL*G*PGRASGAVGAAFF
RSPPMRGRAPLRPAPGAPQPPARSRKLQQLVTKS
LPGRLLPGPRYPGVGRSGWG*ARAGPCVRVRGG
EGRGRRPRRRCGSRDRRLPPRRPWTLSAGVSTL
PAAPPGKAPSRATSGGTWPSAAGKPHSNLPPSPG
FSAKLRGPGSCPRCPEPLCE*AGEA*LECFFFL
IKRVFLNLNF*KFFLRSLQYSLPLRILSCSWSL
FPPQL*IFFTIGGADQG*TSFNCKYNC*NHVGIV
VKMDKKSFEMVLDEIRKVVDLLRYFSWAEPQLK
AMKALQHKMVAVQPTEVVNILNCFTFSKDKLVA
LELLASNIIDAQNSRPIEDLFRVNMSEKKRCKRI
LEQAFKGGCKAPHAMISSCGTIPGNPYPKGRPS
RINGIFPGTPLKKDGEECTNEGKGIAARILGPS
KPPPSTYNPHKPVPYPIPPCRPHATIAPSAYNNA
GLVPLANVIAPPPPPYTPNPVGTENEDLSNPSK
PIQNQTFSTPASQLFSPHGSNPSTPAATPVPTA
SPVKAINHPSASAAATVSGMNLLNTVLPVFPGQV
SSAVHTPQPSIPNPTVIRTPSLPTAPVTSIHST
TTTPVPSIFSGLVSLPGPSATPTAATPTPGPTP
RSTLGSSEAFASTSAPFTSLPFSTSSSAASTSNP
NSASLSSVFAGLPLPLPPTSQGLSNPTPVIAGG
STPSVAGPLGVNSPLLSALKGFLTSNDTNLINS
SALSSAVTSGLASLSSLTLQNSDSSASAPNKCYA
PSAIPTPQRTSTPGLALFPGLPSPVANSTSTPL
TLPVQSPLATAASASTSVPVSCGSSASLLRGPH
PGTSDLHISSTPAATTLPVMIKTEPTSPTPSAFK
GPSHSGNPSHGTLGLSGTLGRAYTSTSVPISLS
ACLNPALSGLSSLSTPLNGSNPLSSISLPPHGS
STPIAPVFTALPSFTSLTNNFPLTGNPSLNPSVS
LPGSLIATSSTAATSTSLPHPSSTAAVLSGLSA
SAPVSAAPFPLNLSTAVPSLFSVTQGPLSSSNP
SYPGFSVSNTPSVTPALPSFPGLQAPSTVAAVTP
LPVAATAPSPAPVLPGFASAFSSNFNSALVAQA
GLSSGLQAAGSSVFPGLLSLPGIPGFPQNPSQS
SLQELQHNAAAQSALLQQVHSASALESYPAQPDG
FPSYPSAPGTPFSLQPSLSQSGWQ*ILLTFILL
QSNIRIA*ELQ*TI*QM*SWPKVGK*E*G*SWR
NCDNNLKIVVAF*SVNTPLCKYADNKLYGEWYLK
SVWRLFTCPIKF*IVYVIYIERILKSRLNSL*P
NTALNMLV*HPLAEVIVVPQTWGLHVALGELLP
LVCMSGSY*HQWELSTLYVSTKGNLRPTVILNF*
Y*QPYILLNLTQNISGK*KWCLM*TIE*LSGVF
N*KYISRTASL*KYK*DLG*FAFKTVFLISAVS
SE*RTLD*LLGHLCYLLYYGSSW*MFTIMGCSIS
ICTLSQMLI*NM*QQIEKTGSVSNYAVCTLFKD
LW*YNMSECH*F*SNNCHMWEVGE*GE*IPICD
*KCKL*TLL*YISGSRSFTFIKMVSGRCC*CILL
QHREQTF*VY**TCMVSF*QFFKINYGYDKYSV
FY*VLG*AF*FCIRARVNY*TFLSKFSCNSVYT
TDCSFV*MIF*CKSHNQHMNRQIYVFKHRM*AIV
*SDTSCWKLPFVFLKSIPIKLLLRL

Frame 1

LGRDWLYCRSP*AGGGCRGNQAGRLALLGLRFS
GVPLCEAVPLFARLLVLPSRRPAPGSCSSW*QR
ACRAACCRGRGIQESGGAAGDEREPDHASGFAAV
RVAAGGRGAGAGRETGASLPAARGL*AQVFLLC
RPLHLGKRLQELPLAAPGPQLQGNRTQTCRRAR
VFLQNSEDLALARGALNHCVNKLGKPDWSVFSF*
*KEFF*ISIFRNFFCEVCNTLYLFASSLARGVF
FLLSYKYFLLSAEQTRDERLLIASITVKTTSG*
*SRWIKSPLKWCWMKLERWLIC*DIFPGPSPS*R
Q*KHYSIKWWLSSQQKWSIYSTVSLSVKTN*LL
LNC*PRTLLMHRILVLLKIYSG*ICLRRNGARE
YLNRLSRGAAKLLML*YLLVEQSQEIHIPKEDLA
A*MEFSQELL*KKMVKNVLTKAKE*LHEFLGHP
NHLLQHIIHINLFLIRYLHADHMQLLHQVLITM
QVWYH*RMS*LHLHLHILLIL*EQRMKTFRIRQN
LYRIKHFPPQQVNSFLLMVLILQHLLQLLFLLH
PQSRQLIIHQHQQLPPFLE*TC*ILSFLCSQGR
SPQPFTHLSHQYQTQQLSEPLHCPLHLLHPSTVQ
PPLLFLPFFLA*CHCQVLLPLLPQPLLPQDLHH
GPLLVPVKHLLLLLHLSLASPFPPALLLLLPAT
QILLHCHQFLQGSLCPYHQHPKAYPTRLL*LLVA
LLPALPVHLV*TVLFCLR*KVF*HPMTPI*STP
LLYPLLSQVGWLHYLLLLFRTLTLLLQPLTSAM
PHQPSLPHRGLPLQGWPCSQACRLPWLTQLPLP*
HCLYSLL*PLLHQLPRQCQLAVAPQPPFCVAPT
QVPQICIFHLPLLQQLFLL*SKLSPQVLLPRPS
KVHLILGIPLMAL*VCQGHWAVHILQHPCPSVYL
LALILHCQVSPA*VLL*MVQILFPLFPFHHMVP
PLPLHQYSLLFLLLLL*PTIFL*LATHLLIRQY
LSQGH**PPHLPLPPPHLSLILAQRQLFSQGFLL
QHQSQQHLSPSTCPLLFPHFSLLLKDLCHLQIP
PIQAFLSLIPQALPLLFPHSRGCRRPLQSQLSH
HYLWLPQPHPQLQSSQDSPQHSVPISTPLLLHKP
VYHLDFKLQAVLFFQAFCPSRVSLGFLRILHNH
PCKNYSIMRLRSQHCYSRSIQLRLWKAIQLSLM
GFLVILQRQEHHFLCNQACPRVGGSEYF*LLFSF
RATSELPENCNEQSDKCEAGQKSENENEGNPGE
IVTTI*KLWLHFKV*TLPYVNMLTINCMENGI*
KVFGDFSPVL*NFELCM*ST*KEY*RVG*TLYSR
IQP*ICLYSIHWQK**LCLRLGGCMWPWGSYYP
WYA*AVPISISGNSVLCMYPQKGT*DPQLFLIS
DINNRTYC*I*LKIFQVSESGA*CRL*NDFQVFS
TESIYPELHPYRNTSKT*DNLPSKQFS*SQQYP
VSEEHLTDSWATSVTYCTMEAPGECLQLWDVVF
LFVL*VKCLYEICDNK*RRLALLVIMQYVLYLRI
CGSIT*VNVINFEVITATCGK*GSKENEFQSVI
KSVNYRLYCSTFQDLEVLLL*RWCPEDVANVFY
FNIGNKLFKYINKPVWLVFNSFLK*TMDMTNILC
FTKCLDRLSNFVYVLELIIEHFYPNLVVTLFIL
LIAHSFK*YFNVKVITNI*TDRFMSLNTECKL*
FNLIPVAGSCHLFFLNLYP*NFF*D

Frame 2

LGATGFIVGPHELVGAVGVTRQGVWRCWGCVFP
ESPYARPCPSSPGSWCSPAAGPLPEAAAAGNKE
PAGPPAAGAEVSRSRAERLGMSASRTMRPGSRR
*GSRPAAEAQVRVERPAPPSPPPVDSERRCFYSA
GRSTWESAFKSYLWRHLALSCRETALKPAAEPG
FFCKTQRTWLLPEVP*TIV*ISWGSLTGVFFLF
NKKSFSESQFLEIFFAKFAILSTSSHPLLLVESF
SSSAINIFYYRRSRPGMNVF*LQV*LLKPRRDS
SQDG*KVL*NGAG*N*KGG*FAEIFFLGRAPVE
GNESITA*NGGCPANRSGQYTQLFHFQ*RQTSCS
*TVSLEHY*CTEFSSY*RFIQGKYV*EETVQEN
T*TGFQGGLQSSSCYDIFLWNNPRKSISQRKT*
PHKWNFPRNSFEKRW*RMY*RRQRNSCTNSWAIQ
TTSFNI*ST*TCSLSDTSMPTTCNYCTKCL*QC
RSGTISECHSSTSTSIYS*SCRNRE*RPFESVK
TYTESNIFHPSKSTLFSSWF*SFNTCCNSCSYCI
PSQGN*SSISISSCHRFWNEPAEYCPSCVPRAG
LLSRSHTSAINTKPNSYQNPFIAHCTCYIHPQY
NHHSCSFHFFWPSVTARSFCHSYRSHSYPRTYTT
VHSWFQ*SICFYFCTFH*PPLFHQLFCCFYQQP
KFCFIVISFCRAPFALTTNIPRPIQPDSCNCWW
LYSQRCRSTWCEQSSFVCVKRFSDIQ*HQFNQLL
CFILCCHKWAGFTIFSYSSEL*LFCFSP*QVLC
PISHPYPTEDFHSRVGPVPRPAVSRG*LNFHSP
DIACTVSFSHCCISFHVSAS*LWLLSLPFAWPPP
RYLRSAYFIYPCCNNSSCYDQN*AHKSYSLGLQ
RSISFWESLSWHFRFVRDIGPCIYFNIRAHQFI
CLP*SCIVRSLQLEYSFKWFKSSFLYFPSTTWFL
HSHCTSIHCSSFFYFFDQQFSFNWQPIS*SVSI
SPRVINSHLIYRCHLHISPSS*LNGSCSLRAFC
FSTSLSSTFPPQPVHCCSLTFLCYSRTSVIFKSL
LSRLFCL*YPKRYPCSSLIPGAAGALYSRSCHT
TTCGCHSPIPSSSPPRIRLSIQFQFQLRSCCTS
RFIIWTSSCRQFCFSRPFVPPGYPWVSSESFTII
LARITA*CGCAVSIVTAGPFSFGSGKLSSSA*W
VS*LSFSARNTIFFATKPVPEWVAVNTFNFYSP
SEQHQNCLRTAMNNLTNVKLAKSRKMRMRVILEK
L*QQFENCGCILKCEHSPM*IC*Q*IVWRMVFK
KCLETFHLSYKILNCVCDLHRKNIKE*VELFIA
EYSLKYACIASTGRSNSCASDLGVACGPGGVTTL
GMHERFLLASVGTQYSVCIHKRELETHSYS*FL
ILTTVHTAEFNSKYFR*VKVVLNVDYRMTFRCF
QLKVYIQNCILIEIQVRLRIICLQNSFPNLSSIQ
*VKNT*LTLGPPLLLTVLWKLLVNVYNYGM*YF
YLYFKSNAYMKYVTTNREDWLC**LCSMYSI*G
SVVV*HE*MSLILK**LPHVGSRGVRRMNSNL*L
KV*TIDSTVVHFRI*KFYFYKDGVRKMLLMYFT
ST*GTNFLSILINLYG*FLTVF*NKLWI*QIFC
VLLSAWIGFLILYTC*S*LLNIFIQI*L*LCLYY
*LLIRLNDILM*KS*PTYEQTDLCL*TQNVSYS
LI*YQLLEVAICFS*IYTHKTSFKI

Sequence NM_025138

ACTTGGGCGCGACTGGCTTTATTGTAGGTCCCCATGAGCTGGTGGGGGCTGTAGGGGTAACCAGGCAGGGCGTCTGGCGCTGTTGGGGCTGCGTTTTTCC
GGAGTCCCCCTATGCGAGGCCGTGCCCCTCTTCGCCCGGCTCCTGGTGCTCCCCAGCCGCCGGCCCGCTCCCGGAAGCTGCAGCAGCTGGTAACAAAGAG
CCTGCCGGGCCGCCTGCTGCCGGGGCCGAGGTATCCAGGAGTCGGGCGGAGCGGCTGGGGATGAGCGCGAGCCGGACCATGCGTCCGGGTTCGCGGCGGT
GAGGGTCGCGGCCGGCGGCCGAGGCGCAGGTGCGGGTCGAGAGACCGGCGCCTCCCTCCCCGCCGCCCGTGGACTCTGAGCGCAGGTGTTTCTACTCTGC
CGGCCGCTCCACCTGGGAAAGCGCCTTCAAGAGCTACCTCTGGCGGCACCTGGCCCTCAGCTGCAGGGAAACCGCACTCAAACCTGCCGCCGAGCCCGGG
TTTTTCTGCAAAACTCAGAGGACCTGGCTCTTGCCCGAGGTGCCCTGAACCATTGTGTGAATAAGCTGGGGAAGCCTGACTGGAGTGTTTTTTCTTTTTA
ATAAAAAGAGTTTTTCTGAATCTCAATTTTTAGAAATTTTTTTTGCGAAGTTTGCAATACTCTCTACCTCTTCGCATCCTCTCTTGCTCGTGGAGTCTTT
TTCCTCCTCAGCTATAAATATTTTTTACTATCGGCGGAGCAGACCAGGGATGAACGTCTTTTAATTGCAAGTATAACTGTTAAAACCACGTCGGGATAGT
AGTCAAGATGGATAAAAAGTCCTTTGAAATGGTGCTGGATGAAATTAGAAAGGCTGTTTTGACAGAATACAAATTAAAAGCAATTGAATATGTGCATGGA
TACTTTTCTAGTGAACAG
GTGGTTGATTTGCTGAGATATTTTTCCTGGGCCGAGCCCCAGTTGAAGGCAATGAAAGCATTACAGCATAAAATGGTGGCTG
TCCAGCCAACAGAAGTGGTCAATATACTCAACTGTTTCACTTTCAGTAAAGACAAACTAGTTGCTCTTGAACTGTTAGCCTCGAACATTATTGATGCACA
GAATTCTCGTCCTATTGAAGATTTATTCAGGGTAAATATGTCTGAGAAGAAACGGTGCAAGAGAATACTTGAACAGGCTTTCAAGGGGGGCTGCAAAGCT
CCTCATGCTATGATATCTTCTTGTGGAACAATCCCAGGAAATCCATATCCCAAAGGAAGACCTAGCCGCATAAATGGAATTTTCCCAGGAACTCCTTTGA
AAAAAGATGGTGAAGAATGTACTAACGAAGGCAAAGGAATAGCTGCACGAATTCTTGGGCCATCCAAACCACCTCCTTCAACATATAATCCACATAAACC
TGTTCCTTATCCGATACCTCCATGCCGACCACATGCAACTATTGCACCAAGTGCTTATAACAATGCAGGTCTGGTACCATTAGCGAATGTCATAGCTCCA
CCTCCACCTCCATATACTCCTAATCCTGTAGGAACAGAGAATGAAGACCTTTCGAATCCGTCAAAACCTATACAGAATCAAACATTTTCCACCCCAGCAA
GTCAACTCTTTTCTCCTCATGGTTCTAATCCTTCAACACCTGCTGCAACTCCTGTTCCTACTGCATCCCCAGTCAAGGCAATTAATCATCCATCAGCATC
AGCAGCTGCCACCGTTTCTGGAATGAACCTGCTGAATACTGTCCTTCCTGTGTTCCCAGGGCAGGTCTCCTCAGCCGTTCACACACCTCAGCCATCAATA
CCAAACCCAACAGTTATCAGAACCCCTTCATTGCCCACTGCACCTGTTACATCCATCCACAGTACAACCACCACTCCTGTTCCTTCCATTTTTTCTGGCC
TAGTGTCACTGCCAGGTCCTTCTGCCACTCCTACCGCAGCCACTCCTACCCCAGGACCTACACCACGGTCCACTCTTGGTTCCAGTGAAGCATTTGCTTC
TACTTCTGCACCTTTCACTAGCCTCCCCTTTTCCACCAGCTCTTCTGCTGCTTCTACCAGCAACCCAAATTCTGCTTCATTGTCATCAGTTTTTGCAGGG
CTCCCTTTGCCCTTACCACCAACATCCCAAGGCCTATCCAACCCGACTCCTGTAATTGCTGGTGGCTCTACTCCCAGCGTTGCCGGTCCACTTGGTGTGA
ACAGTCCTCTTTTGTCTGCGTTAAAAGGTTTTCTGACATCCAATGACACCAATTTAATCAACTCCTCTGCTTTATCCTCTGCTGTCACAAGTGGGCTGGC
TTCACTATCTTCTCTTACTCTTCAGAACTCTGACTCTTCTGCTTCAGCCCCTAACAAGTGCTATGCCCCATCAGCCATCCCTACCCCACAGAGGACTTCC
ACTCCAGGGTTGGCCCTGTTCCCAGGCCTGCCGTCTCCCGTGGCTAACTCAACTTCCACTCCCCTGACATTGCCTGTACAGTCTCCTTTAGCCACTGCTG
CATCAGCTTCCACGTCAGTGCCAGTTAGCTGTGGCTCCTCAGCCTCCCTTTTGCGTGGCCCCCACCCAGGTACCTCAGATCTGCATATTTCATCTACCCC
TGCTGCAACAACTCTTCCTGTTATGATCAAAACTGAGCCCACAAGTCCTACTCCCTCGGCCTTCAAAGGTCCATCTCATTCTGGGAATCCCTCTCATGGC
ACTTTAGGTTTGTCAGGGACATTGGGCCGTGCATATACTTCAACATCCGTGCCCATCAGTTTATCTGCTTGCCTTAATCCTGCATTGTCAGGTCTCTCCA
GCTTGAGTACTCCTTTAAATGGTTCAAATCCTCTTTCCTCTATTTCCCTTCCACCACATGGTTCCTCCACTCCCATTGCACCAGTATTCACTGCTCTTCC
TTCTTTTACTTCTTTGACCAACAATTTTCCTTTAACTGGCAACCCATCTCTTAATCCGTCAGTATCTCTCCCAGGGTCATTAATAGCCACCTCATCTACC
GCTGCCACCTCCACATCTCTCCCTCATCCTAGCTCAACGGCAGCTGTTCTCTCAGGGCTTTCTGCTTCAGCACCAGTCTCAGCAGCACCTTTCCCCCTCA
ACCTGTCCACTGCTGTTCCCTCACTTTTCTCTGTTACTCAAGGACCTCTGTCATCTTCAAATCCCTCCTATCCAGGCTTTTCTGTCTCTAATACCCCAAG
CGTTACCCCTGCTCTTCCCTCATTCCCGGGGCTGCAGGCGCCCTCTACAGTCGCAGCTGTCACACCACTACCTGTGGCTGCCACAGCCCCATCCCCAGCT
CCAGTCCTCCCAGGATTCGCCTCAGCATTCAGTTCCAATTTCAACTCCGCTCTTGTTGCACAAGCCGGTTTATCATCTGGACTTCAAGCTGCAGGCAGTT
CTGTTTTTCCAGGCCTTTTGTCCCTCCCGGGTATCCCTGGGTTTCCTCAGAATCCTTCACAATCATCCTTGCAAGAATTACAGCATAATGCGGCTGCGCA
GTCAGCATTGTTACAGCAGGTCCATTCAGCTTCGGCTCTGGAAAGCTATCCAGCTCAGCCTGATGGGTTTCCTAGTTATCCTTCAGCGCCAGGAACACCA
TTTTCTTTGCAACCAAGCCTGTCCCAGAGTGGGTGGCAGTGAATACTTTTAACTTTTATTCTCCTTCAGAGCAACATCAGAATTGCCTGAGAACTGCAAT
GAACAATCTGACAAATGTGAAGCTGGCCAAAAGTCGGAAAATGAGAATGAGGGTAATCCTGGAGAAATTGTGACAACAATTTGAAAATTGTGGTTGCATT
TTAAAGTGTGAACACTCCCCTATGTAAATATGCTGACAATAAATTGTATGGAGAATGGTATTTAAAAAGTGTTTGGAGACTTTTCACCTGTCCTATAAAA
TTTTGAATTGTGTATGTGATCTACATAGAAAGAATATTAAAGAGTAGGTTGAACTCTTTATAGCCGAATACAGCCTTAAATATGCTTGTATAGCATCCAC
TGGCAGAAGTAATAGTTGTGCCTCAGACTTGGGGGTTGCATGTGGCCCTGGGGGAGTTACTACCCTTGGTATGCATGAGCGGTTCCTATTAGCATCAGTG
GGAACTCAGTACTCTGTATGTATCCACAAAAGGGAACTTGAGACCCACAGTTATTCTTAATTTCTGATATTAACAACCGTACATACTGCTGAATTTAACT
CAAAATATTTCAGGTAAGTGAAAGTGGTGCTTAATGTAGACTATAGAATGACTTTCAGGTGTTTTCAACTGAAAGTATATATCCAGAACTGCATCCTTAT
AGAAATACAAGTAAGACTTAGGATAATTTGCCTTCAAAACAGTTTTCCTAATCTCAGCAGTATCCAGTGAGTGAAGAACACTTGACTGACTCTTGGGCCA
CCTCTGTTACTTACTGTACTATGGAAGCTCCTGGTGAATGTTTACAATTATGGGATGTAGTATTTCTATTTGTACTTTAAGTCAAATGCTTATATGAAAT
ATGTGACAACAAATAGAGAAGACTGGCTCTGTTAGTAATTATGCAGTATGTACTCTATTTAAGGATCTGTGGTAGTATAACATGAGTGAATGTCATTAAT
TTTGAAGTAATAACTGCCACATGTGGGAAGTAGGGGAGTAAGGAGAATGAATTCCAATCTGTGATTAAAAGTGTAAACTATAGACTCTACTGTAGTACAT
TTCAGGATCTAGAAGTTTTACTTTTATAAAGATGGTGTCCGGAAGATGTTGCTAATGTATTTTACTTCAACATAGGGAACAAACTTTTTAAGTATATTAA
TAAACCTGTATGGTTAGTTTTTAACAGTTTTTTAAAATAAACTATGGATATGACAAATATTCTGTGTTTTACTAAGTGCTTGGATAGGCTTTCTAATTTT
GTATACGTGCTAGAGTTAATTATTGAACATTTTTATCCAAATTTAGTTGTAACTCTGTTTATACTACTGATTGCTCATTCGTTTAAATGATATTTTAATG
TAAAAGTCATAACCAACATATGAACAGACAGATTTATGTCTTTAAACACAGAATGTAAGCTATAGTTTAATCTGATACCAGTTGCTGGAAGTTGCCATTT
GTTTTTCTTAAATCTATACCCATAAAACTTCTTTTAAGATTA
  • refseq_C13orf23.F2 (forward primer)
  • refseq_C13orf23.R2 (reverse primer)
  • Single exon skipping

Frame 0

TWARLALL*VPMSWWGL*G*PGRASGAVGAAFF
RSPPMRGRAPLRPAPGAPQPPARSRKLQQLVTKS
LPGRLLPGPRYPGVGRSGWG*ARAGPCVRVRGG
EGRGRRPRRRCGSRDRRLPPRRPWTLSAGVSTL
PAAPPGKAPSRATSGGTWPSAAGKPHSNLPPSPG
FSAKLRGPGSCPRCPEPLCE*AGEA*LECFFFL
IKRVFLNLNF*KFFLRSLQYSLPLRILSCSWSL
FPPQL*IFFTIGGADQG*TSFNCKYNC*NHVGIV
VKMDKKSFEMVLDEIRKAVLTEYKLKAIEYVHG
YFSSEQ
VVDLLRYFSWAEPQLKAMKALQHKMVA
VQPTEVVNILNCFTFSKDKLVALELLASNIIDAQ
NSRPIEDLFRVNMSEKKRCKRILEQAFKGGCKA
PHAMISSCGTIPGNPYPKGRPSRINGIFPGTPL
KKDGEECTNEGKGIAARILGPSKPPPSTYNPHKP
VPYPIPPCRPHATIAPSAYNNAGLVPLANVIAP
PPPPYTPNPVGTENEDLSNPSKPIQNQTFSTPA
SQLFSPHGSNPSTPAATPVPTASPVKAINHPSAS
AAATVSGMNLLNTVLPVFPGQVSSAVHTPQPSI
PNPTVIRTPSLPTAPVTSIHSTTTTPVPSIFSG
LVSLPGPSATPTAATPTPGPTPRSTLGSSEAFAS
TSAPFTSLPFSTSSSAASTSNPNSASLSSVFAG
LPLPLPPTSQGLSNPTPVIAGGSTPSVAGPLGV
NSPLLSALKGFLTSNDTNLINSSALSSAVTSGLA
SLSSLTLQNSDSSASAPNKCYAPSAIPTPQRTS
TPGLALFPGLPSPVANSTSTPLTLPVQSPLATA
ASASTSVPVSCGSSASLLRGPHPGTSDLHISSTP
AATTLPVMIKTEPTSPTPSAFKGPSHSGNPSHG
TLGLSGTLGRAYTSTSVPISLSACLNPALSGLS
SLSTPLNGSNPLSSISLPPHGSSTPIAPVFTALP
SFTSLTNNFPLTGNPSLNPSVSLPGSLIATSST
AATSTSLPHPSSTAAVLSGLSASAPVSAAPFPL
NLSTAVPSLFSVTQGPLSSSNPSYPGFSVSNTPS
VTPALPSFPGLQAPSTVAAVTPLPVAATAPSPA
PVLPGFASAFSSNFNSALVAQAGLSSGLQAAGS
SVFPGLLSLPGIPGFPQNPSQSSLQELQHNAAAQ
SALLQQVHSASALESYPAQPDGFPSYPSAPGTP
FSLQPSLSQSGWQ*ILLTFILLQSNIRIA*ELQ
*TI*QM*SWPKVGK*E*G*SWRNCDNNLKIVVAF
*SVNTPLCKYADNKLYGEWYLKSVWRLFTCPIK
F*IVYVIYIERILKSRLNSL*PNTALNMLV*HP
LAEVIVVPQTWGLHVALGELLPLVCMSGSY*HQW
ELSTLYVSTKGNLRPTVILNF*Y*QPYILLNLT
QNISGK*KWCLM*TIE*LSGVFN*KYISRTASL
*KYK*DLG*FAFKTVFLISAVSSE*RTLD*LLGH
LCYLLYYGSSW*MFTIMGCSISICTLSQMLI*N
M*QQIEKTGSVSNYAVCTLFKDLW*YNMSECH*
F*SNNCHMWEVGE*GE*IPICD*KCKL*TLL*YI
SGSRSFTFIKMVSGRCC*CILLQHREQTF*VY*
*TCMVSF*QFFKINYGYDKYSVFY*VLG*AF*F
CIRARVNY*TFLSKFSCNSVYTTDCSFV*MIF*C
KSHNQHMNRQIYVFKHRM*AIV*SDTSCWKLPF
VFLKSIPIKLLLRL

Frame 1

LGRDWLYCRSP*AGGGCRGNQAGRLALLGLRFS
GVPLCEAVPLFARLLVLPSRRPAPGSCSSW*QR
ACRAACCRGRGIQESGGAAGDEREPDHASGFAAV
RVAAGGRGAGAGRETGASLPAARGL*AQVFLLC
RPLHLGKRLQELPLAAPGPQLQGNRTQTCRRAR
VFLQNSEDLALARGALNHCVNKLGKPDWSVFSF*
*KEFF*ISIFRNFFCEVCNTLYLFASSLARGVF
FLLSYKYFLLSAEQTRDERLLIASITVKTTSG*
*SRWIKSPLKWCWMKLERLF*QNTN*KQLNMCMD
TFLVNR
WLIC*DIFPGPSPS*RQ*KHYSIKWWL
SSQQKWSIYSTVSLSVKTN*LLLNC*PRTLLMH
RILVLLKIYSG*ICLRRNGAREYLNRLSRGAAKL
LML*YLLVEQSQEIHIPKEDLAA*MEFSQELL*
KKMVKNVLTKAKE*LHEFLGHPNHLLQHIIHIN
LFLIRYLHADHMQLLHQVLITMQVWYH*RMS*LH
LHLHILLIL*EQRMKTFRIRQNLYRIKHFPPQQ
VNSFLLMVLILQHLLQLLFLLHPQSRQLIIHQH
QQLPPFLE*TC*ILSFLCSQGRSPQPFTHLSHQY
QTQQLSEPLHCPLHLLHPSTVQPPLLFLPFFLA
*CHCQVLLPLLPQPLLPQDLHHGPLLVPVKHLL
LLLHLSLASPFPPALLLLLPATQILLHCHQFLQG
SLCPYHQHPKAYPTRLL*LLVALLPALPVHLV*
TVLFCLR*KVF*HPMTPI*STPLLYPLLSQVGW
LHYLLLLFRTLTLLLQPLTSAMPHQPSLPHRGLP
LQGWPCSQACRLPWLTQLPLP*HCLYSLL*PLL
HQLPRQCQLAVAPQPPFCVAPTQVPQICIFHLP
LLQQLFLL*SKLSPQVLLPRPSKVHLILGIPLMA
L*VCQGHWAVHILQHPCPSVYLLALILHCQVSP
A*VLL*MVQILFPLFPFHHMVPPLPLHQYSLLF
LLLLL*PTIFL*LATHLLIRQYLSQGH**PPHLP
LPPPHLSLILAQRQLFSQGFLLQHQSQQHLSPS
TCPLLFPHFSLLLKDLCHLQIPPIQAFLSLIPQ
ALPLLFPHSRGCRRPLQSQLSHHYLWLPQPHPQL
QSSQDSPQHSVPISTPLLLHKPVYHLDFKLQAV
LFFQAFCPSRVSLGFLRILHNHPCKNYSIMRLR
SQHCYSRSIQLRLWKAIQLSLMGFLVILQRQEHH
FLCNQACPRVGGSEYF*LLFSFRATSELPENCN
EQSDKCEAGQKSENENEGNPGEIVTTI*KLWLH
FKV*TLPYVNMLTINCMENGI*KVFGDFSPVL*N
FELCM*ST*KEY*RVG*TLYSRIQP*ICLYSIH
WQK**LCLRLGGCMWPWGSYYPWYA*AVPISIS
GNSVLCMYPQKGT*DPQLFLISDINNRTYC*I*L
KIFQVSESGA*CRL*NDFQVFSTESIYPELHPY
RNTSKT*DNLPSKQFS*SQQYPVSEEHLTDSWA
TSVTYCTMEAPGECLQLWDVVFLFVL*VKCLYEI
CDNK*RRLALLVIMQYVLYLRICGSIT*VNVIN
FEVITATCGK*GSKENEFQSVIKSVNYRLYCST
FQDLEVLLL*RWCPEDVANVFYFNIGNKLFKYIN
KPVWLVFNSFLK*TMDMTNILCFTKCLDRLSNF
VYVLELIIEHFYPNLVVTLFILLIAHSFK*YFN
VKVITNI*TDRFMSLNTECKL*FNLIPVAGSCHL
FFLNLYP*NFF*D

Frame 2

LGATGFIVGPHELVGAVGVTRQGVWRCWGCVFP
ESPYARPCPSSPGSWCSPAAGPLPEAAAAGNKE
PAGPPAAGAEVSRSRAERLGMSASRTMRPGSRR
*GSRPAAEAQVRVERPAPPSPPPVDSERRCFYSA
GRSTWESAFKSYLWRHLALSCRETALKPAAEPG
FFCKTQRTWLLPEVP*TIV*ISWGSLTGVFFLF
NKKSFSESQFLEIFFAKFAILSTSSHPLLLVESF
SSSAINIFYYRRSRPGMNVF*LQV*LLKPRRDS
SQDG*KVL*NGAG*N*KGCFDRIQIKSN*ICAW
ILF**T
GG*FAEIFFLGRAPVEGNESITA*NGGC
PANRSGQYTQLFHFQ*RQTSCS*TVSLEHY*CT
EFSSY*RFIQGKYV*EETVQENT*TGFQGGLQS
SSCYDIFLWNNPRKSISQRKT*PHKWNFPRNSFE
KRW*RMY*RRQRNSCTNSWAIQTTSFNI*ST*T
CSLSDTSMPTTCNYCTKCL*QCRSGTISECHSS
TSTSIYS*SCRNRE*RPFESVKTYTESNIFHPSK
STLFSSWF*SFNTCCNSCSYCIPSQGN*SSISI
SSCHRFWNEPAEYCPSCVPRAGLLSRSHTSAIN
TKPNSYQNPFIAHCTCYIHPQYNHHSCSFHFFWP
SVTARSFCHSYRSHSYPRTYTTVHSWFQ*SICF
YFCTFH*PPLFHQLFCCFYQQPKFCFIVISFCR
APFALTTNIPRPIQPDSCNCWWLYSQRCRSTWCE
QSSFVCVKRFSDIQ*HQFNQLLCFILCCHKWAG
FTIFSYSSEL*LFCFSP*QVLCPISHPYPTEDF
HSRVGPVPRPAVSRG*LNFHSPDIACTVSFSHCC
ISFHVSAS*LWLLSLPFAWPPPRYLRSAYFIYP
CCNNSSCYDQN*AHKSYSLGLQRSISFWESLSW
HFRFVRDIGPCIYFNIRAHQFICLP*SCIVRSLQ
LEYSFKWFKSSFLYFPSTTWFLHSHCTSIHCSS
FFYFFDQQFSFNWQPIS*SVSISPRVINSHLIY
RCHLHISPSS*LNGSCSLRAFCFSTSLSSTFPPQ
PVHCCSLTFLCYSRTSVIFKSLLSRLFCL*YPK
RYPCSSLIPGAAGALYSRSCHTTTCGCHSPIPS
SSPPRIRLSIQFQFQLRSCCTSRFIIWTSSCRQF
CFSRPFVPPGYPWVSSESFTIILARITA*CGCA
VSIVTAGPFSFGSGKLSSSA*WVS*LSFSARNT
IFFATKPVPEWVAVNTFNFYSPSEQHQNCLRTAM
NNLTNVKLAKSRKMRMRVILEKL*QQFENCGCI
LKCEHSPM*IC*Q*IVWRMVFKKCLETFHLSYK
ILNCVCDLHRKNIKE*VELFIAEYSLKYACIAST
GRSNSCASDLGVACGPGGVTTLGMHERFLLASV
GTQYSVCIHKRELETHSYS*FLILTTVHTAEFN
SKYFR*VKVVLNVDYRMTFRCFQLKVYIQNCILI
EIQVRLRIICLQNSFPNLSSIQ*VKNT*LTLGP
PLLLTVLWKLLVNVYNYGM*YFYLYFKSNAYMK
YVTTNREDWLC**LCSMYSI*GSVVV*HE*MSLI
LK**LPHVGSRGVRRMNSNL*LKV*TIDSTVVH
FRI*KFYFYKDGVRKMLLMYFTST*GTNFLSIL
INLYG*FLTVF*NKLWI*QIFCVLLSAWIGFLIL
YTC*S*LLNIFIQI*L*LCLYY*LLIRLNDILM
*KS*PTYEQTDLCL*TQNVSYSLI*YQLLEVAI
CFS*IYTHKTSFKI

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