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Sequence NM_031448
GCGGGGCTGGGGAGCTGGGCGGGGAGCCCGGGGCCTGCCAGGCCCGGGCTGCAGCCGCGTCTGATCGCCGAGCGCGCCGCGTAGACCTCCGCTCCCCCAGGGGGGGCTGTCGGGGGGCTGTTAGGTGCCTGGATGACAAGTGGACAGTTTAAGCCGGTTCCTCAGATCCTAATGGAGCTGCCCCCTGCCGAGCAACAGAG
GCTCTTTAACGAAGCCGCAGCCATCATCAGGCACCTGGAGTGGACGGACGCCGTGCAGCTGACCGCGCTGGTCATGGGCAGCGAGGCCCTGCAGCAGCAG
CTGCTGGCCATGCTGGTGAACTACGTCACCAAGGAGCTGCGGGCCGAGATCCAGTATGATGACTAGGCCGCACCTCCGGGGAGGTGGGGGGCCCCTTTAA
ATGACTCTGTGATTCTGAAGAGGTGGCTTGGGAGTTGGGAGAAGCCCAGCGGATGCCCCCTGGGGAATCTCCACATCATCAGTGTATTACTAGTAATGTC
CCGCTGGAGAGGCCACCGCTGTGCAGTGTCATGTTCCAGAAATTACTGATGAAGCAGCATGTGTTGGTGGCATGTGCACTGGCCTGCCATGACAGCCCTC
TGACTGGCCCCCCAGTGAAGAGTAAAGGCCTGCCTGCCGCAGGCTTCGGAGGCGTCTGCTGAGTCCTCTCACCCGCATGGGTCTGGGGAAGTGATCACGC
TCAGCCGACGGTCTGACCACACTTCATCCTCCCCCCGGGGCCTTCTCATCTTGGGAGATGACTCCTCTTCAGAGCACCTGCTGCAGGACTGGATCCCACC
CCCCTGCAGGTCCTGGGGTCTCAGGGCCTTGGAGCAGCCCATGCTGGAATCATGTTTACCTCCTAGTGCAACCGTCCCCTACCCAGGGACTGTCGAATGG
CCCCACGGAGGGGACGGGCGGCCTGCTGAGTGAAGCCACAAATACCGAGTGGACTTGACCCCGGCCCCCACTAGGCTGCACACCTAGACTCGCCCTGCCA
GGGCCTCGCTCTTCCCATCTGAAAAGTCCTGGTAGTTCTTGAGGTTTACTTCTCAAATGAAATATTTTTAGTAAAAAGTACAGGTATATCTCGGAGATAT
TGTGGGTTCAGTTCCAGACCACCTCGGTAAAGCCAACATCACAATAAAGCAAGGAAGCGCATTGTTTTAGTTTCCCAGTGCATCTAAGTCATGTTTACTG
CATATTGCAGTCCACTAAATGTGCAATAGCATTATGTCTAACAAATGTACAAACCTTAATTTAAAAATATTTACTGTTCAAAATGCTGACACAGAAACGC
AAAGTGAGCACATGCTGTTGGAAAATGGTGCCAAATAGACTTGCCTGATGCCAGGCTGCTACAAACCTTCAATTTAAAAAAAAAACACAGTATTCACAAA
GCATAGTAGAATGAGGTATGCCTGTATTGCTCTTTCTGAAGTGGTGTGATATAAACCATCTCTAAGAAATGTTTCTACCCTAAAGATTTCCCCAGTACAG
TCAGCTCTCCGTAACTGTGGTCTCCACATTTAGATCCAACCAGCCTTGGATAGGAAATATTTGAAAAAAGAAATTGCATTGGTACTGAACACGTACAGAC
CTTTTTTTCTTGCCATTATTCCCTAAACAATATGGTGTAGCATATTTACATAGCATTTATATTGTATTTGGTATTATAAGAAATCTAGAGATGATTTAAA
TTATACAGGAAGGTGTGCGTAGGTTACGTGCAAACGCTATGCCATTGCCCATCAGGGACTTGAGCATCCTCAGATGTCGGTGTCTGAGGGTTGAGGTTGC
AGTCCTGGAACCCATCCCCCATGGATACTGAGGCATAGCTGTACTGTGTGTTTTCACTTTGCTTTCAGAACTACGACTTGAATGTGATCGATTACAATAA
ATGTTTTTCTAAAAA
- refseq_C19orf12.F2 (forward primer)
- refseq_C19orf12.R2 (reverse primer)
- Single exon skipping
Frame 0
AGLGSWAGSPGPARPGLQPRLIAERAA*TSAPPGGAVGGLLGAWMTSGQFKPVPQILMELPPAEQQR
LFNEAAAIIRHLEWTDAVQLTALVMGSEALQQQ
LLAMLVNYVTKELRAEIQYDD*AAPPGRWGAPL
NDSVILKRWLGSWEKPSGCPLGNLHIISVLLVMS
RWRGHRCAVSCSRNY**SSMCWWHVHWPAMTAL
*LAPQ*RVKACLPQASEASAESSHPHGSGEVIT
LSRRSDHTSSSPRGLLILGDDSSSEHLLQDWIPP
PCRSWGLRALEQPMLESCLPPSATVPYPGTVEW
PHGGDGRPAE*SHKYRVDLTPAPTRLHT*TRPA
RASLFPSEKSW*FLRFTSQMKYF**KVQVYLGDI
VGSVPDHLGKANITIKQGSALF*FPSASKSCLL
HIAVH*MCNSIMSNKCTNLNLKIFTVQNADTET
QSEHMLLENGAK*TCLMPGCYKPSI*KKNTVFTK
HSRMRYACIALSEVV*YKPSLRNVSTLKISPVQ
SALRNCGLHI*IQPALDRKYLKKEIALVLNTYR
PFFLAIIP*TIWCSIFT*HLYCIWYYKKSRDDLN
YTGRCA*VTCKRYAIAHQGLEHPQMSVSEG*GC
SPGTHPPWILRHSCTVCFHFAFRTTT*M*SITI
NVFLK
Frame 1
RGWGAGRGARGLPGPGCSRV*SPSAPRRPPLPQGGLSGGC*VPG*QVDSLSRFLRS*WSCPLPSNR
GSLTKPQPSSGTWSGRTPCS*PRWSWAARPCSSS
CWPCW*TTSPRSCGPRSSMMTRPHLRGGGGPL*
MTL*F*RGGLGVGRSPADAPWGISTSSVYY**C
PAGEATAVQCHVPEITDEAACVGGMCTGLP*QPS
DWPPSEE*RPACRRLRRRLLSPLTRMGLGK*SR
SADGLTTLHPPPGAFSSWEMTPLQSTCCRTGSH
PPAGPGVSGPWSSPCWNHVYLLVQPSPTQGLSNG
PTEGTGGLLSEATNTEWT*PRPPLGCTPRLALP
GPRSSHLKSPGSS*GLLLK*NIFSKKYRYISEI
LWVQFQTTSVKPTSQ*SKEAHCFSFPVHLSHVYC
ILQSTKCAIALCLTNVQTLI*KYLLFKMLTQKR
KVSTCCWKMVPNRLA*CQAATNLQFKKKTQYSQ
SIVE*GMPVLLFLKWCDINHL*EMFLP*RFPQYS
QLSVTVVSTFRSNQPWIGNI*KKKLHWY*TRTD
LFFLPLFPKQYGVAYLHSIYIVFGIIRNLEMI*
IIQEGVRRLRANAMPLPIRDLSILRCRCLRVEVA
VLEPIPHGY*GIAVLCVFTLLSELRLECDRLQ*
MFF*K
Frame 2
GAGELGGEPGACQARAAAASDRRARRVDLRSPRGGCRGAVRCLDDKWTV*AGSSDPNGAAPCRATE
AL*RSRSHHQAPGVDGRRAADRAGHGQRGPAAA
AAGHAGELRHQGAAGRDPV**LGRTSGEVGGPFK
*LCDSEEVAWELGEAQRMPPGESPHHQCITSNV
PLERPPLCSVMFQKLLMKQHVLVACALACHDSP
LTGPPVKSKGLPAAGFGGVC*VLSPAWVWGSDHA
QPTV*PHFILPPGPSHLGR*LLFRAPAAGLDPT
PLQVLGSQGLGAAHAGIMFTS*CNRPLPRDCRM
APRRGRAAC*VKPQIPSGLDPGPH*AAHLDSPCQ
GLALPI*KVLVVLEVYFSNEIFLVKSTGISRRY
CGFSSRPPR*SQHHNKARKRIVLVSQCI*VMFT
AYCSPLNVQ*HYV*QMYKP*FKNIYCSKC*HRNA
K*AHAVGKWCQIDLPDARLLQTFNLKKKHSIHK
A**NEVCLYCSF*SGVI*TISKKCFYPKDFPST
VSSP*LWSPHLDPTSLG*EIFEKRNCIGTEHVQT
FFSCHYSLNNMV*HIYIAFILYLVL*EI*R*FK
LYRKVCVGYVQTLCHCPSGT*ASSDVGV*GLRL
QSWNPSPMDTEA*LYCVFSLCFQNYDLNVIDYNK
CFSK
Sequence NM_001031726
GCGGGGCTGGGGAGCTGGGCGGGGAGCCCGGGGCCTGCCAGGCCCGGGCTGCAGCCGCGTCTGATCGCCGAGCGCGCCGCGTAGACCTCCGCTCCCCCAGGCCCGCCACGATGACTATCATGGTGGAGGACATCATGAAGCTGCTGTGCTCCCTTTCTGGGGAGAGGAAGATGAAGGCGGCTGTCAAGCACTCTGGGAAG
GGTGCCCTGGTCACAGGGGCCATGGCCTTCGTCGGGGGTTTGGTGGGCGGCCCACCGGGACTCGCCGTTGGGGGGGCTGTCGGGGGGCTGTTAGGTGCCT
GGATGACAAGTGGACAGTTTAAGCCGGTTCCTCAGATCCTAATGGAGCTGCCCCCTGCCGAGCAACAGAGGCTCTTTAACGAAGCCGCAGCCATCATCAG
GCCCTGCAGCAGCAGCTGCTGGCCATGCTGGTGAACTACGTCACCAAGGAGCTGCGGGCCGAGATCCAGTATGATGACTAGGCCGCACCTCCGGGGAGGT
GGGGGGCCCCTTTAAATGACTCTGTGATTCTGAAGAGGTGGCTTGGGAGTTGGGAGAAGCCCAGCGGATGCCCCCTGGGGAATCTCCACATCATCAGTGT
ATTACTAGTAATGTCCCGCTGGAGAGGCCACCGCTGTGCAGTGTCATGTTCCAGAAATTACTGATGAAGCAGCATGTGTTGGTGGCATGTGCACTGGCCT
GCCATGACAGCCCTCTGACTGGCCCCCCAGTGAAGAGTAAAGGCCTGCCTGCCGCAGGCTTCGGAGGCGTCTGCTGAGTCCTCTCACCCGCATGGGTCTG
GGGAAGTGATCACGCTCAGCCGACGGTCTGACCACACTTCATCCTCCCCCCGGGGCCTTCTCATCTTGGGAGATGACTCCTCTTCAGAGCACCTGCTGCA
GGACTGGATCCCACCCCCCTGCAGGTCCTGGGGTCTCAGGGCCTTGGAGCAGCCCATGCTGGAATCATGTTTACCTCCTAGTGCAACCGTCCCCTACCCA
GGGACTGTCGAATGGCCCCACGGAGGGGACGGGCGGCCTGCTGAGTGAAGCCACAAATACCGAGTGGACTTGACCCCGGCCCCCACTAGGCTGCACACCT
AGACTCGCCCTGCCAGGGCCTCGCTCTTCCCATCTGAAAAGTCCTGGTAGTTCTTGAGGTTTACTTCTCAAATGAAATATTTTTAGTAAAAAGTACAGGT
ATATCTCGGAGATATTGTGGGTTCAGTTCCAGACCACCTCGGTAAAGCCAACATCACAATAAAGCAAGGAAGCGCATTGTTTTAGTTTCCCAGTGCATCT
AAGTCATGTTTACTGCATATTGCAGTCCACTAAATGTGCAATAGCATTATGTCTAACAAATGTACAAACCTTAATTTAAAAATATTTACTGTTCAAAATG
CTGACACAGAAACGCAAAGTGAGCACATGCTGTTGGAAAATGGTGCCAAATAGACTTGCCTGATGCCAGGCTGCTACAAACCTTCAATTTAAAAAAAAAA
CACAGTATTCACAAAGCATAGTAGAATGAGGTATGCCTGTATTGCTCTTTCTGAAGTGGTGTGATATAAACCATCTCTAAGAAATGTTTCTACCCTAAAG
ATTTCCCCAGTACAGTCAGCTCTCCGTAACTGTGGTCTCCACATTTAGATCCAACCAGCCTTGGATAGGAAATATTTGAAAAAAGAAATTGCATTGGTAC
TGAACACGTACAGACCTTTTTTTCTTGCCATTATTCCCTAAACAATATGGTGTAGCATATTTACATAGCATTTATATTGTATTTGGTATTATAAGAAATC
TAGAGATGATTTAAATTATACAGGAAGGTGTGCGTAGGTTACGTGCAAACGCTATGCCATTGCCCATCAGGGACTTGAGCATCCTCAGATGTCGGTGTCT
GAGGGTTGAGGTTGCAGTCCTGGAACCCATCCCCCATGGATACTGAGGCATAGCTGTACTGTGTGTTTTCACTTTGCTTTCAGAACTACGACTTGAATGT
GATCGATTACAATAAATGTTTTTCTAAAAA
- refseq_C19orf12.F2 (forward primer)
- refseq_C19orf12.R2 (reverse primer)
- Single exon skipping
Frame 0
AGLGSWAGSPGPARPGLQPRLIAERAA*TSAPPGPPR*LSWWRTS*SCCAPFLGRGR*RRLSSTLGR
VPWSQGPWPSSGVWWAAHRDSPLGGLSGGC*VP
G*QVDSLSRFLRS*WSCPLPSNRGSLTKPQPSS
GPAAAAAGHAGELRHQGAAGRDPV**LGRTSGEV
GGPFK*LCDSEEVAWELGEAQRMPPGESPHHQC
ITSNVPLERPPLCSVMFQKLLMKQHVLVACALA
CHDSPLTGPPVKSKGLPAAGFGGVC*VLSPAWVW
GSDHAQPTV*PHFILPPGPSHLGR*LLFRAPAA
GLDPTPLQVLGSQGLGAAHAGIMFTS*CNRPLP
RDCRMAPRRGRAAC*VKPQIPSGLDPGPH*AAHL
DSPCQGLALPI*KVLVVLEVYFSNEIFLVKSTG
ISRRYCGFSSRPPR*SQHHNKARKRIVLVSQCI
*VMFTAYCSPLNVQ*HYV*QMYKP*FKNIYCSKC
*HRNAK*AHAVGKWCQIDLPDARLLQTFNLKKK
HSIHKA**NEVCLYCSF*SGVI*TISKKCFYPK
DFPSTVSSP*LWSPHLDPTSLG*EIFEKRNCIGT
EHVQTFFSCHYSLNNMV*HIYIAFILYLVL*EI
*R*FKLYRKVCVGYVQTLCHCPSGT*ASSDVGV
*GLRLQSWNPSPMDTEA*LYCVFSLCFQNYDLNV
IDYNKCFSK
Frame 1
RGWGAGRGARGLPGPGCSRV*SPSAPRRPPLPQARHDDYHGGGHHEAAVLPFWGEEDEGGCQALWE
GCPGHRGHGLRRGFGGRPTGTRRWGGCRGAVRCL
DDKWTV*AGSSDPNGAAPCRATEAL*RSRSHHQ
ALQQQLLAMLVNYVTKELRAEIQYDD*AAPPGR
WGAPLNDSVILKRWLGSWEKPSGCPLGNLHIISV
LLVMSRWRGHRCAVSCSRNY**SSMCWWHVHWP
AMTAL*LAPQ*RVKACLPQASEASAESSHPHGS
GEVITLSRRSDHTSSSPRGLLILGDDSSSEHLLQ
DWIPPPCRSWGLRALEQPMLESCLPPSATVPYP
GTVEWPHGGDGRPAE*SHKYRVDLTPAPTRLHT
*TRPARASLFPSEKSW*FLRFTSQMKYF**KVQV
YLGDIVGSVPDHLGKANITIKQGSALF*FPSAS
KSCLLHIAVH*MCNSIMSNKCTNLNLKIFTVQN
ADTETQSEHMLLENGAK*TCLMPGCYKPSI*KKN
TVFTKHSRMRYACIALSEVV*YKPSLRNVSTLK
ISPVQSALRNCGLHI*IQPALDRKYLKKEIALV
LNTYRPFFLAIIP*TIWCSIFT*HLYCIWYYKKS
RDDLNYTGRCA*VTCKRYAIAHQGLEHPQMSVS
EG*GCSPGTHPPWILRHSCTVCFHFAFRTTT*M
*SITINVFLK
Frame 2
GAGELGGEPGACQARAAAASDRRARRVDLRSPRPATMTIMVEDIMKLLCSLSGERKMKAAVKHSGK
GALVTGAMAFVGGLVGGPPGLAVGGAVGGLLGA
WMTSGQFKPVPQILMELPPAEQQRLFNEAAAIIR
PCSSSCWPCW*TTSPRSCGPRSSMMTRPHLRGG
GGPL*MTL*F*RGGLGVGRSPADAPWGISTSSV
YY**CPAGEATAVQCHVPEITDEAACVGGMCTGL
P*QPSDWPPSEE*RPACRRLRRRLLSPLTRMGL
GK*SRSADGLTTLHPPPGAFSSWEMTPLQSTCC
RTGSHPPAGPGVSGPWSSPCWNHVYLLVQPSPTQ
GLSNGPTEGTGGLLSEATNTEWT*PRPPLGCTP
RLALPGPRSSHLKSPGSS*GLLLK*NIFSKKYR
YISEILWVQFQTTSVKPTSQ*SKEAHCFSFPVHL
SHVYCILQSTKCAIALCLTNVQTLI*KYLLFKM
LTQKRKVSTCCWKMVPNRLA*CQAATNLQFKKK
TQYSQSIVE*GMPVLLFLKWCDINHL*EMFLP*R
FPQYSQLSVTVVSTFRSNQPWIGNI*KKKLHWY
*TRTDLFFLPLFPKQYGVAYLHSIYIVFGIIRN
LEMI*IIQEGVRRLRANAMPLPIRDLSILRCRCL
RVEVAVLEPIPHGY*GIAVLCVFTLLSELRLEC
DRLQ*MFF*K
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