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Sequences

Sequence NM_213632

ACCAACCCCGCCAGGTCGCGGGCACTCCCACCAGCTCGCAGCCCTCCTTCGGACCCTCGATCCGAGAGCGCCCCAGCCAGAGCTTGTGCCCATGCCCCTG
TCCCGCAGGGGAAGGGGATCCCGCCTGGCCGGCTCTCGAGCGGCGACTAGTAACCTCCCGCGGGCGACAGCCCTCTCCCCAAAGCCACGCGCAACTCCTC
ACCCGGCGGCGCCATTCCTCCGAGCAGGTCCCGCCGGAGTCCGAGCCGCGGGCTGACTTCCGCTCGGGAAAGTGGCTCCAAGAGCCCGCCACAGGGGACG
CCAGGGACTCGCGGCAGGCTCTGCGGGCCAGGATGAGTTCTAAGCACAGGATCTGTAGTCAGGAAGAAGTAGTGATCCCCTGTGCCTATGACAGTGATTC
AGAAAGTGTGGATTTGGAGCTGAGCAACTTAGAGATTATTAAAAAAGGCTCAAGTAGCATTGAACTGACAGACTTGGACATCCCTGACATCCCTGGACTC
CATTGTGAGCCCCTGTCACATAGCCCCAGACACCTGACCCAACAGGACCCGCTCAGTGAGGCCATTGTTGAGAAACTGATCCAGTCCATCCAGAAGGTTT
TCAATGGTGAGCTAAAGGGTGAACTTGAAAAGCTGAAATTCCTAGGAGATCTGTCTTCTCTCAGCCAAGCTTTACCTTATGATGAAACCGCAAAATCATT
CATTCACAGCCACATAGCAGACATTGTGCATACCTTAAATGTACTGGTACAAGAGGAACGCCCGCATTCTCTGTCCAGTTCCATGCGCCAGGAGGTCTTT
GTCACCATCGCTGATCTCAGTTACCAAGATGTCCATTTGCTGTTGGGCTCTGAAGATCGAGCTGAGTTGTTCAGTCTTACCATCAAGAGTATAATCACTC
TGCCCTCTGTAAGGACCCTTACCCAGATACAGGAAATCATGCCCAATGGGACCTGCAACACAGAGTGTCTTTACAGGCAGACGTTTCAGGCATTCTCTGA
GATGCTCCAGAGTTTGGTGGTAAAAGACCCACATTTGGAAAATCTTGACACCATTATTAAGCTTCCACTGCGATTCCAAAGACTTGGACACCTAGTGGCT
CTGATGGCACTGCTCTGTGGGGACCCACAGGAAAAGGTGGCTGAGGAGGCTGCAGAGGGCATTCACTCCCTGCTGCATATCACCCTGAGGCTGAAGTATA
TCACTCATGACAAGAAAGATCAGCAAAACTTGAAAAGAGCATTGACAAAATGTCGAGAATTCCTGGAGCTCCACAGCTCTGCCGCTAAATGCTTCTACAA
CTGTCCCTTCAGAATTGCCCAGGTCTTTGAAGGTTTTCTTGATTCAAATGAGCTCTGCCAGTTTATAATGACTACATTTGATACCCTGAAAACCCTGAAA
CATCCCTGCATCCAGCGATCAGCAGGAGAATTACTGCTAACTTTGGCAAAAAATACAGAGTCCCAATTTGAGAAGGTGCCAGAAATTATGGGAGTTATCT
GTGCCCAGTTATCCATAATCAGCCAGCCTAGAGTCCGCCAACAAATCATAAATACCGTGAGTTTATTTATATCCAGACCCAAGTACACAGATATAGTGCT
CAGCTTCCTTCTGTGTCATCCAGTGCCGTATAACAGGCACCTGGCTGAGGTGTGGAGAATGCTGTCGGTGGAGCTTCCCAGCACGACCTGGATTCTGTGG
AGGCTCCTGAGGAAGCTGCAGAAATGCCATAATGAGCCTGCACAGGAGAAGATGGCATATGTGGCTGTGGCTGTAAGCCCTTGAGCTTTGCCCCTCTAAT
GTCAGCCCCTCCCAGGATGACTGACCCCACACCCCAGTGGAGAGTGTTCCTTTTGGTCCCTCTCAAAGCCTGCATCTGTCATCCATTTATAAGTCTACAG
TGTTTCTGCCAAGGGATTGACATGGAGGGAGCTGGGGAGAGTGAAGGAGTCAGAATGCCTTAATACAAACTCGTCCAACCTGC
  • refseq_C20orf132.F1 (forward primer)
  • refseq_C20orf132.R1 (reverse primer)
  • Single exon skipping

Frame 0

TNPARSRALPPARSPPSDPRSESAPARACAHAP
VPQGKGIPPGRLSSGD**PPAGDSPLPKATRNSS
PGGAIPPSRSRRSPSRGLTSARESGSKSPPQGT
PGTRGRLCGPG*VLSTGSVVRKK**SPVPMTVI
QKVWIWS*AT*RLLKKAQVALN*QTWTSLTSLDS
IVSPCHIAPDT*PNRTRSVRPLLRN*SSPSRRF
SMVS*RVNLKS*NS*EICLLSAKLYLMMKPQNH
SFTAT*QTLCIP*MYWYKRNARILCPVPCARRSL
SPSLISVTKMSICCWALKIELSCSVLPSRV*SL
CPL*GPLPRYRKSCPMGPATQSVFTGRRFRHSL
RCSRVWW*KTHIWKILTPLLSFHCDSKDLDT*WL
*WHCSVGTHRKRWLRRLQRAFTPCCISP*G*SI
SLMTRKISKT*KEH*QNVENSWSSTALPLNAST
TVPSELPRSLKVFLIQMSSASL**LHLIP*KP*N
IPASSDQQENYC*LWQKIQSPNLRRCQKLWELS
VPSYP*SASLESANKS*IP*VYLYPDPSTQI*C
SASFCVIQCRITGTWLRCGECCRWSFPARPGFCG
GS*GSCRNAIMSLHRRRWHMWLWL*ALELCPSN
VSPSQDD*PHTPVESVPFGPSQSLHLSSIYKST
VFLPRD*HGGSWGE*RSQNALIQTRPTC

Frame 1

PTPPGRGHSHQLAALLRTLDPRAPQPELVPMPL
SRRGRGSRLAGSRAATSNLPRATALSPKPRATP
HPAAPFLRAGPAGVRAAG*LPLGKVAPRARHRGR
QGLAAGSAGQDEF*AQDL*SGRSSDPLCL*Q*F
RKCGFGAEQLRDY*KRLK*H*TDRLGHP*HPWT
PL*APVT*PQTPDPTGPAQ*GHC*ETDPVHPEGF
QW*AKG*T*KAEIPRRSVFSQPSFTL**NRKII
HSQPHSRHCAYLKCTGTRGTPAFSVQFHAPGGL
CHHR*SQLPRCPFAVGL*RSS*VVQSYHQEYNHS
ALCKDPYPDTGNHAQWDLQHRVSLQADVSGIL*
DAPEFGGKRPTFGKS*HHY*ASTAIPKTWTPSG
SDGTALWGPTGKGG*GGCRGHSLPAAYHPEAEVY
HS*QERSAKLEKSIDKMSRIPGAPQLCR*MLLQ
LSLQNCPGL*RFS*FK*ALPVYNDYI*YPENPE
TSLHPAISRRITANFGKKYRVPI*EGARNYGSYL
CPVIHNQPA*SPPTNHKYREFIYIQTQVHRYSA
QLPSVSSSAV*QAPG*GVENAVGGASQHDLDSV
EAPEEAAEMP**ACTGEDGICGCGCKPLSFAPLM
SAPPRMTDPTPQWRVFLLVPLKACICHPFISLQ
CFCQGIDMEGAGESEGVRMP*YKLVQP

Frame 2

QPRQVAGTPTSSQPSFGPSIRERPSQSLCPCPC
PAGEGDPAWPALERRLVTSRGRQPSPQSHAQLL
TRRRHSSEQVPPESEPRADFRSGKWLQEPATGD
ARDSRQALRARMSSKHRICSQEEVVIPCAYDSDS
ESVDLELSNLEIIKKGSSSIELTDLDIPDIPGL
HCEPLSHSPRHLTQQDPLSEAIVEKLIQSIQKV
FNGELKGELEKLKFLGDLSSLSQALPYDETAKSF
IHSHIADIVHTLNVLVQEERPHSLSSSMRQEVF
VTIADLSYQDVHLLLGSEDRAELFSLTIKSIIT
LPSVRTLTQIQEIMPNGTCNTECLYRQTFQAFSE
MLQSLVVKDPHLENLDTIIKLPLRFQRLGHLVA
LMALLCGDPQEKVAEEAAEGIHSLLHITLRLKY
ITHDKKDQQNLKRALTKCREFLELHSSAAKCFYN
CPFRIAQVFEGFLDSNELCQFIMTTFDTLKTLK
HPCIQRSAGELLLTLAKNTESQFEKVPEIMGVI
CAQLSIISQPRVRQQIINTVSLFISRPKYTDIVL
SFLLCHPVPYNRHLAEVWRMLSVELPSTTWILW
RLLRKLQKCHNEPAQEKMAYVAVAVSP*ALPL*
CQPLPG*LTPHPSGECSFWSLSKPASVIHL*VYS
VSAKGLTWRELGRVKESECLNTNSSNL

Sequence NM_213631

ACCAACCCCGCCAGGTCGCGGGCACTCCCACCAGCTCGCAGCCCTCCTTCGGACCCTCGATCCGAGAGCGCCCCAGCCAGAGCTTGTGCCCATGCCCCTG
TCCCGCAGGGGAAGGGGATCCCGCCTGGCCGGCTCTCGAGCGGCGACTAGTAACCTCCCGCGGGCGACAGCCCTCTCCCCAAAGCCACGCGCAACTCCTC
ACCCGGCGGCGCCATTCCTCCGAGCAGGTCCCGCCGGAGTCCGAGCCGCGGGCTGACTTCCGCTCGGGAAAGTGGCTCCAAGAGCCCGCCACAGGGGACG
CCAGGGACTCGCGGCAGGCTCTGCGGGCCAGGATGAGTTCTAAGCACAGGATCTGTAGTCAGGAAGAAGTAGTGATCCCCTGTGCCTATGACAGTGATTC
AGAAAGTGTGGATTTGGAGCTGAGCAACTTAGAGATTATTAAAAAAGGCTCAAGTAGCATTGAACTGACAGACTTGGACATCCCTGACATCCCTGGACTC
CATTGTGAGCCCCTGTCACATAGCCCCAGACACCTGACCCAACAGGACCCGCTCAGTGAGGCCATTGTTGAGAAACTGATCCAGTCCATCCAGAAGGTTT
TCAATGGTGAGCTAAAGGGTGAACTTGAAAAGCTGAAATTCCTAGGAGATCTGTCTTCTCTCAGCCAAGCTTTACCTTATGATGAAACCGCAAAATCATT
CATTCACAGCCACATAGCAGACATTGTGCATACCTTAAATGTACTGGTACAAGAGGAACGCCCGCATTCTCTGTCCAGTTCCATGCGCCAGGAGGTCTTT
GTCACCATCGCTGATCTCAGTTACCAAGATGTCCATTTGCTGTTGGGCTCTGAAGATCGAGCTGAGTTGTTCAGTCTTACCATCAAGAGTATAATCACTC
TGCCCTCTGTAAGGACCCTTACCCAGATACAGGAAATCATGCCCAATGGGACCTGCAACACAGAGTGTCTTTACAGGCAGACGTTTCAGGCATTCTCTGA
GATGCTCCAGAGTTTGGTGGTAAAAGACCCACATTTGGAAAATCTTGACACCATTATTAAGCACTTGGTCCCCTGGTTACAGTCAGTCAAAGACCATGAG
CGGGAACGGGCCACGGCCAGCATGGCTCAAGTTCTGAAGTGCCTATCCAAACATCTCAACTTGAAGCTTCCACTGCGATTCCAAAGACTTGGACACCTAG
TGGCTCTGATGGCACTGCTCTGTGGGGACCCACAGGAAAAGGTGGCTGAGGAGGCTGCAGAGGGCATTCACTCCCTGCTGCATATCACCCTGAGGCTGAA
GTATATCACTCATGACAAGAAAGATCAGCAAAACTTGAAAAGAGCATTGACAAAATGTCGAGAATTCCTGGAGCTCCACAGCTCTGCCGCTAAATGCTTC
TACAACTGTCCCTTCAGAATTGCCCAGGTCTTTGAAGGTTTTCTTGATTCAAATGAGCTCTGCCAGTTTATAATGACTACATTTGATACCCTGAAAACCC
TGAAACATCCCTGCATCCAGCGATCAGCAGGAGAATTACTGCTAACTTTGGCAAAAAATACAGAGTCCCAATTTGAGAAGGTGCCAGAAATTATGGGAGT
TATCTGTGCCCAGTTATCCATAATCAGCCAGCCTAGAGTCCGCCAACAAATCATAAATACCGTGAGTTTATTTATATCCAGACCCAAGTACACAGATATA
GTGCTCAGCTTCCTTCTGTGTCATCCAGTGCCGTATAACAGGCACCTGGCTGAGGTGTGGAGAATGCTGTCGGTGGAGCTTCCCAGCACGACCTGGATTC
TGTGGAGGCTCCTGAGGAAGCTGCAGAAATGCCATAATGAGCCTGCACAGGAGAAGATGGCATATGTGGCTGTGGCTGTAAGCCCTTGAGCTTTGCCCCT
CTAATGTCAGCCCCTCCCAGGATGACTGACCCCACACCCCAGTGGAGAGTGTTCCTTTTGGTCCCTCTCAAAGCCTGCATCTGTCATCCATTTATAAGTC
TACAGTGTTTCTGCCAAGGGATTGACATGGAGGGAGCTGGGGAGAGTGAAGGAGTCAGAATGCCTTAATACAAACTCGTCCAACCTGC
  • refseq_C20orf132.F1 (forward primer)
  • refseq_C20orf132.R1 (reverse primer)
  • Single exon skipping

Frame 0

TNPARSRALPPARSPPSDPRSESAPARACAHAP
VPQGKGIPPGRLSSGD**PPAGDSPLPKATRNSS
PGGAIPPSRSRRSPSRGLTSARESGSKSPPQGT
PGTRGRLCGPG*VLSTGSVVRKK**SPVPMTVI
QKVWIWS*AT*RLLKKAQVALN*QTWTSLTSLDS
IVSPCHIAPDT*PNRTRSVRPLLRN*SSPSRRF
SMVS*RVNLKS*NS*EICLLSAKLYLMMKPQNH
SFTAT*QTLCIP*MYWYKRNARILCPVPCARRSL
SPSLISVTKMSICCWALKIELSCSVLPSRV*SL
CPL*GPLPRYRKSCPMGPATQSVFTGRRFRHSL
RCSRVWW*KTHIWKILTPLLSTWSPGYSQSKTMS
GNGPRPAWLKF*SAYPNIST*SFHCDSKDLDT*
WL*WHCSVGTHRKRWLRRLQRAFTPCCISP*G*
SISLMTRKISKT*KEH*QNVENSWSSTALPLNAS
TTVPSELPRSLKVFLIQMSSASL**LHLIP*KP
*NIPASSDQQENYC*LWQKIQSPNLRRCQKLWE
LSVPSYP*SASLESANKS*IP*VYLYPDPSTQI*
CSASFCVIQCRITGTWLRCGECCRWSFPARPGF
CGGS*GSCRNAIMSLHRRRWHMWLWL*ALELCP
SNVSPSQDD*PHTPVESVPFGPSQSLHLSSIYKS
TVFLPRD*HGGSWGE*RSQNALIQTRPTC

Frame 1

PTPPGRGHSHQLAALLRTLDPRAPQPELVPMPL
SRRGRGSRLAGSRAATSNLPRATALSPKPRATP
HPAAPFLRAGPAGVRAAG*LPLGKVAPRARHRGR
QGLAAGSAGQDEF*AQDL*SGRSSDPLCL*Q*F
RKCGFGAEQLRDY*KRLK*H*TDRLGHP*HPWT
PL*APVT*PQTPDPTGPAQ*GHC*ETDPVHPEGF
QW*AKG*T*KAEIPRRSVFSQPSFTL**NRKII
HSQPHSRHCAYLKCTGTRGTPAFSVQFHAPGGL
CHHR*SQLPRCPFAVGL*RSS*VVQSYHQEYNHS
ALCKDPYPDTGNHAQWDLQHRVSLQADVSGIL*
DAPEFGGKRPTFGKS*HHY*ALGPLVTVSQRP*
AGTGHGQHGSSSEVPIQTSQLEASTAIPKTWTPS
GSDGTALWGPTGKGG*GGCRGHSLPAAYHPEAE
VYHS*QERSAKLEKSIDKMSRIPGAPQLCR*ML
LQLSLQNCPGL*RFS*FK*ALPVYNDYI*YPENP
ETSLHPAISRRITANFGKKYRVPI*EGARNYGS
YLCPVIHNQPA*SPPTNHKYREFIYIQTQVHRY
SAQLPSVSSSAV*QAPG*GVENAVGGASQHDLDS
VEAPEEAAEMP**ACTGEDGICGCGCKPLSFAP
LMSAPPRMTDPTPQWRVFLLVPLKACICHPFIS
LQCFCQGIDMEGAGESEGVRMP*YKLVQP

Frame 2

QPRQVAGTPTSSQPSFGPSIRERPSQSLCPCPC
PAGEGDPAWPALERRLVTSRGRQPSPQSHAQLL
TRRRHSSEQVPPESEPRADFRSGKWLQEPATGD
ARDSRQALRARMSSKHRICSQEEVVIPCAYDSDS
ESVDLELSNLEIIKKGSSSIELTDLDIPDIPGL
HCEPLSHSPRHLTQQDPLSEAIVEKLIQSIQKV
FNGELKGELEKLKFLGDLSSLSQALPYDETAKSF
IHSHIADIVHTLNVLVQEERPHSLSSSMRQEVF
VTIADLSYQDVHLLLGSEDRAELFSLTIKSIIT
LPSVRTLTQIQEIMPNGTCNTECLYRQTFQAFSE
MLQSLVVKDPHLENLDTIIKHLVPWLQSVKDHE
RERATASMAQVLKCLSKHLNLKLPLRFQRLGHL
VALMALLCGDPQEKVAEEAAEGIHSLLHITLRLK
YITHDKKDQQNLKRALTKCREFLELHSSAAKCF
YNCPFRIAQVFEGFLDSNELCQFIMTTFDTLKT
LKHPCIQRSAGELLLTLAKNTESQFEKVPEIMGV
ICAQLSIISQPRVRQQIINTVSLFISRPKYTDI
VLSFLLCHPVPYNRHLAEVWRMLSVELPSTTWI
LWRLLRKLQKCHNEPAQEKMAYVAVAVSP*ALPL
*CQPLPG*LTPHPSGECSFWSLSKPASVIHL*V
YSVSAKGLTWRELGRVKESECLNTNSSNL

Sequence NM_152503

ACCAACCCCGCCAGGTCGCGGGCACTCCCACCAGCTCGCAGCCCTCCTTCGGACCCTCGATCCGAGAGCGCCCCAGCCAGAGCTTGTGCCCATGCCCCTG
TCCCGCAGGGGAAGGGGATCCCGCCTGGCCGGCTCTCGAGCGGCGACTAGTAACCTCCCGCGGGCGACAGCCCTCTCCCCAAAGCCACGCGCAACTCCTC
ACCCGGCGGCGCCATTCCTCCGAGCAGGTCCCGCCGGAGTCCGAGCCGCGGGCTGACTTCCGCTCGGGAAAGTGGCTCCAAGAGCCCGCCACAGGGGACG
CCAGGGACTCGCGGCAGGCTCTGCGGGCCAGGATGAGTTCTAAGCACAGGATCTGTAGTCAGGAAGAAGTAGTGATCCCCTGTGCCTATGACAGTGATTC
AGAAAGTGTGGATTTGGAGCTGAGCAACTTAGAGATTATTAAAAAAGGCTCAAGTAGCATTGAACTGACAGACTTGGACATCCCTGACATCCCTGGACTC
CATTGTGAGCCCCTGTCACATAGCCCCAGACACCTGACCCAACAGGACCCGCTCAGTGAGGCCATTGTTGAGAAACTGATCCAGTCCATCCAGAAGGTTT
TCAATGGTGAGCTAAAGGGTGAACTTGAAAAGCTGAAATTCCTAGGAGATCTGTCTTCTCTCAGCCAAGCTTTACCTTATGATGAAACCGCAAAATCATT
CATTCACAGCCACATAGCAGACATTGTGCATACCTTAAATGTACTGGTACAAGAGGAACGCCCGCATTCTCTGTCCAGTTCCATGCGCCAGGAGGTCTTT
GTCACCATCGCTGATCTCAGTTACCAAGATGTCCATTTGCTGTTGGGCTCTGAAGATCGAGCTGAGTTGTTCAGTCTTACCATCAAGAGTATAATCACTC
TGCCCTCTGTAAGGACCCTTACCCAGATACAGGAAATCATGCCCAATGGGACCTGCAACACAGAGTGTCTTTACAGGCAGACGTTTCAGGCATTCTCTGA
GATGCTCCAGAGTTTGGTGGTAAAAGACCCACATTTGGAAAATCTTGACACCATTATTAAGCACTTGGTCCCCTGGTTACAGTCAGTCAAAGACCATGAG
CGGGAACGGGCCACGGCCAGCATGGCTCAAGTTCTGAAGTGCCTATCCAAACATCTCAACTTGAAGCTTCCACTGCGATTCCAAAGACTTGGACACCTAG
TGGCTCTGATGGCACTGCTCTGTGGGGACCCACAGGAAAAGGTGGCTGAGGAGGCTGCAGAGGGCATTCACTCCCTGCTGCATATCACCCTGAGGCTGAA
GTATATCACTCATGACAAGAAAGATCAGCAAAACTTGAAAAGAGCATTGACAAAATGTCGAGAATTCCTGGAGCTCCACAGCTCTGCCGCTAAATGCTTC
TACAACTGTCCCTTCAGAATTGCCCAGGTCTTTGAAGGTTTTCTTGATTCAAATGAGCTCTGCCAGTTTATAATGACTACATTTGATACCCTGAAAACCC
TGAAACATCCCTGCATCCAGCGATCAGCAGGAGAATTACTGCTAACTTTGGCAAAAAATACAGAGTCCCAATTTGAGAAGGTGCCAGAAATTATGGGAGT
TATCTGTGCCCAGTTATCCATAATCAGCCAGCCTAGAGTCCGCCAACAAATCATAAATACCGTGAGTTTATTTATATCCAGACCCAAGTACACAGATATA
GTGCTCAGCTTCCTTCTGTGTCATCCAGTGCCGTATAACAGGCACCTGGCTGAGGTGTGGAGAATGCTGTCGGTGGAGCTTCCCAGCACGACCTGGATTC
TGTGGAGGCTCCTGAGGAAGCTGCAGAAATGCCATAATGAGCCTGCACAGGAGAAGATGGCATATGTGGCTGTGGCTGCAACAGATGCCCTTTATGAGGT
GTTTTTGGGAAACAGGCTTCGAGCAGCTACGTTCCGACTCTTTCCTCAGCTTCTCATGACACTGCTTATCCAGATTCATCACAGCATCGGCCTCACCATG
TCTGATGTCGACATCCCAAGTGGCCTGTACACAGAACAGGAAGTGCCTTCAGAGGTCACCCCTTTGTGCTTTGCCATGCAGGCTACAAAGACTCTCCTCC
TCAGAACATGCTGCTTGCAGGAATTCAACATCATGGAAAAGAATAAAGGATGGGCTCTCCTGGGAGGAAAAGATGGCCATCTTCAGGGACTATTTCTCCT
TGCCAACGCATTGCTGGAAAGAAATCAGCTCCTTGCACAGAAGGTCATGTACTTATTAGTCCCTCTTCTTAACCGAGGGAATGATAAACATAAACTCACA
TCTGCAGGCTTTTTTGTGGAGCTTCTCCGGAGTCCAGTGGCCAAGAGACTGCCCAGCATATACTCTGTTGCCCGCTTTAAAGACTGGCTACAAGATGGAA
ATCATCTCTTTAGAATTCTCGGCCTGAGGGGACTGTACAATCTTGTTGGACACCAGGAGATGAGAGAAGACATCAAGAGCCTGTTGCCATACATTGTAGA
CAGCTTGCGTGAAACCGATGAGAAGATCGTTCTGTCAGCCATCCAGATACTCCTGCAACTTGTTAGAACAATGGATTTCACTACCCTGGCTGCCATGATG
AGGACCCTGTTCTCCTTATTTGGTGATGTGAGATCTGATGTTCATCGTTTCTCCGTGACTCTCTTTGGAGCCGCCATAAAGTCTGTAAAAAACCCAGATA
AGAAGAGTATAGAGAACCAAGTCCTGGACAGCTTGGTCCCACTACTTCTGTATTCTCAGGATGAAAATGATGCAGTAGCTGAGGAGAGCAGGCAAGTCCT
AACTATATGTGCCCAGTTCCTGAAGTGGAAGCTGCCCCAAGAAGTGTACTCCAAAGATCCCTGGCACATCAAACCTACTGAAGCAGGAACAATCTGCAGA
TTCTTTGAAAAAAAGTGCAAGGGGAAAATTAACATCCTAGAACAAACACTGATGTACTCCAAGAACCCAAAACTTCCCATCAGAAGATCAGCAGTCTTGT
TTGTAGGCCTTTTATCGAAGTACATGGATCACAATGAGCTCAGGAGGATGGGTACTGACTGGATAGAGGACGATCTGAGAGACCTGCTGTGTGACCCTGA
GCCCTCGCTGTGCATCATCGCTTCCCAGACTCTGTTACTAGTCCAGATGGCGAGGGCCGAACCAAAACCTAAGCAGAGAGTGAACTGGTTGCAGAAGCTC
ATGGGCAGGTCCTCTGCCTAGAAACACAAGGCAAGCAACATCAGAGACAGAATCTTGCTATGTTGTGCGGCAAGCTAGTCTTGAACTCATGGCCTCAAGT
CATCCTCCTGTGTCAGCCTCCCAAAGTGCTGGGATTACAAGCATGCACCACGGCACCCAGCAGAATTCCAGTCTTGAGAAACAGGTCAAGGACAGCTTCA
AAAGAGATTCTAAATAAATGTTAATGTTACAATGTTA
  • refseq_C20orf132.F1 (forward primer)
  • refseq_C20orf132.R1 (reverse primer)
  • Single exon skipping

Frame 0

TNPARSRALPPARSPPSDPRSESAPARACAHAP
VPQGKGIPPGRLSSGD**PPAGDSPLPKATRNSS
PGGAIPPSRSRRSPSRGLTSARESGSKSPPQGT
PGTRGRLCGPG*VLSTGSVVRKK**SPVPMTVI
QKVWIWS*AT*RLLKKAQVALN*QTWTSLTSLDS
IVSPCHIAPDT*PNRTRSVRPLLRN*SSPSRRF
SMVS*RVNLKS*NS*EICLLSAKLYLMMKPQNH
SFTAT*QTLCIP*MYWYKRNARILCPVPCARRSL
SPSLISVTKMSICCWALKIELSCSVLPSRV*SL
CPL*GPLPRYRKSCPMGPATQSVFTGRRFRHSL
RCSRVWW*KTHIWKILTPLLSTWSPGYSQSKTMS
GNGPRPAWLKF*SAYPNIST*SFHCDSKDLDT*
WL*WHCSVGTHRKRWLRRLQRAFTPCCISP*G*
SISLMTRKISKT*KEH*QNVENSWSSTALPLNAS
TTVPSELPRSLKVFLIQMSSASL**LHLIP*KP
*NIPASSDQQENYC*LWQKIQSPNLRRCQKLWE
LSVPSYP*SASLESANKS*IP*VYLYPDPSTQI*
CSASFCVIQCRITGTWLRCGECCRWSFPARPGF
CGGS*GSCRNAIMSLHRRRWHMWLWLQQMPFMR
CFWETGFEQLRSDSFLSFS*HCLSRFITASASPC
LMSTSQVACTQNRKCLQRSPLCALPCRLQRLSS
SEHAACRNSTSWKRIKDGLSWEEKMAIFRDYFS
LPTHCWKEISSLHRRSCTY*SLFLTEGMININSH
LQAFLWSFSGVQWPRDCPAYTLLPALKTGYKME
IISLEFSA*GDCTILLDTRR*EKTSRACCHTL*
TACVKPMRRSFCQPSRYSCNLLEQWISLPWLP**
GPCSPYLVM*DLMFIVSP*LSLEPP*SL*KTQI
RRV*RTKSWTAWSHYFCILRMKMMQ*LRRAGKS
*LYVPSS*SGSCPKKCTPKIPGTSNLLKQEQSAD
SLKKSARGKLTS*NKH*CTPRTQNFPSEDQQSC
L*AFYRSTWITMSSGGWVLTG*RTI*ETCCVTL
SPRCASSLPRLCY*SRWRGPNQNLSRE*TGCRSS
WAGPLPRNTRQATSETESCYVVRQASLELMASS
HPPVSASQSAGITSMHHGTQQNSSLEKQVKDSF
KRDSK*MLMLQC

Frame 1

PTPPGRGHSHQLAALLRTLDPRAPQPELVPMPL
SRRGRGSRLAGSRAATSNLPRATALSPKPRATP
HPAAPFLRAGPAGVRAAG*LPLGKVAPRARHRGR
QGLAAGSAGQDEF*AQDL*SGRSSDPLCL*Q*F
RKCGFGAEQLRDY*KRLK*H*TDRLGHP*HPWT
PL*APVT*PQTPDPTGPAQ*GHC*ETDPVHPEGF
QW*AKG*T*KAEIPRRSVFSQPSFTL**NRKII
HSQPHSRHCAYLKCTGTRGTPAFSVQFHAPGGL
CHHR*SQLPRCPFAVGL*RSS*VVQSYHQEYNHS
ALCKDPYPDTGNHAQWDLQHRVSLQADVSGIL*
DAPEFGGKRPTFGKS*HHY*ALGPLVTVSQRP*
AGTGHGQHGSSSEVPIQTSQLEASTAIPKTWTPS
GSDGTALWGPTGKGG*GGCRGHSLPAAYHPEAE
VYHS*QERSAKLEKSIDKMSRIPGAPQLCR*ML
LQLSLQNCPGL*RFS*FK*ALPVYNDYI*YPENP
ETSLHPAISRRITANFGKKYRVPI*EGARNYGS
YLCPVIHNQPA*SPPTNHKYREFIYIQTQVHRY
SAQLPSVSSSAV*QAPG*GVENAVGGASQHDLDS
VEAPEEAAEMP**ACTGEDGICGCGCNRCPL*G
VFGKQASSSYVPTLSSASHDTAYPDSSQHRPHH
V*CRHPKWPVHRTGSAFRGHPFVLCHAGYKDSPP
QNMLLAGIQHHGKE*RMGSPGRKRWPSSGTISP
CQRIAGKKSAPCTEGHVLISPSS*PRE**T*TH
ICRLFCGASPESSGQETAQHILCCPL*RLATRWK
SSL*NSRPEGTVQSCWTPGDERRHQEPVAIHCR
QLA*NR*EDRSVSHPDTPATC*NNGFHYPGCHD
EDPVLLIW*CEI*CSSFLRDSLWSRHKVCKKPR*
EEYREPSPGQLGPTTSVFSG*K*CSS*GEQASP
NYMCPVPEVEAAPRSVLQRSLAHQTY*SRNNLQ
IL*KKVQGEN*HPRTNTDVLQEPKTSHQKISSLV
CRPFIEVHGSQ*AQEDGY*LDRGRSERPAV*P*
ALAVHHRFPDSVTSPDGEGRTKT*AESELVAEA
HGQVLCLETQGKQHQRQNLAMLCGKLVLNSWPQV
ILLCQPPKVLGLQACTTAPSRIPVLRNRSRTAS
KEILNKC*CYNV

Frame 2

QPRQVAGTPTSSQPSFGPSIRERPSQSLCPCPC
PAGEGDPAWPALERRLVTSRGRQPSPQSHAQLL
TRRRHSSEQVPPESEPRADFRSGKWLQEPATGD
ARDSRQALRARMSSKHRICSQEEVVIPCAYDSDS
ESVDLELSNLEIIKKGSSSIELTDLDIPDIPGL
HCEPLSHSPRHLTQQDPLSEAIVEKLIQSIQKV
FNGELKGELEKLKFLGDLSSLSQALPYDETAKSF
IHSHIADIVHTLNVLVQEERPHSLSSSMRQEVF
VTIADLSYQDVHLLLGSEDRAELFSLTIKSIIT
LPSVRTLTQIQEIMPNGTCNTECLYRQTFQAFSE
MLQSLVVKDPHLENLDTIIKHLVPWLQSVKDHE
RERATASMAQVLKCLSKHLNLKLPLRFQRLGHL
VALMALLCGDPQEKVAEEAAEGIHSLLHITLRLK
YITHDKKDQQNLKRALTKCREFLELHSSAAKCF
YNCPFRIAQVFEGFLDSNELCQFIMTTFDTLKT
LKHPCIQRSAGELLLTLAKNTESQFEKVPEIMGV
ICAQLSIISQPRVRQQIINTVSLFISRPKYTDI
VLSFLLCHPVPYNRHLAEVWRMLSVELPSTTWI
LWRLLRKLQKCHNEPAQEKMAYVAVAATDALYEV
FLGNRLRAATFRLFPQLLMTLLIQIHHSIGLTM
SDVDIPSGLYTEQEVPSEVTPLCFAMQATKTLL
LRTCCLQEFNIMEKNKGWALLGGKDGHLQGLFLL
ANALLERNQLLAQKVMYLLVPLLNRGNDKHKLT
SAGFFVELLRSPVAKRLPSIYSVARFKDWLQDG
NHLFRILGLRGLYNLVGHQEMREDIKSLLPYIVD
SLRETDEKIVLSAIQILLQLVRTMDFTTLAAMM
RTLFSLFGDVRSDVHRFSVTLFGAAIKSVKNPD
KKSIENQVLDSLVPLLLYSQDENDAVAEESRQVL
TICAQFLKWKLPQEVYSKDPWHIKPTEAGTICR
FFEKKCKGKINILEQTLMYSKNPKLPIRRSAVL
FVGLLSKYMDHNELRRMGTDWIEDDLRDLLCDPE
PSLCIIASQTLLLVQMARAEPKPKQRVNWLQKL
MGRSSA*KHKASNIRDRILLCCAAS*S*THGLK
SSSCVSLPKCWDYKHAPRHPAEFQS*ETGQGQLQ
KRF*INVNVTML

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