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Sequences

Sequence NM_015560

CACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTGGCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAG TCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTGGTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAA AGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTCCTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTA CCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTATCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCC AAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATTGTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATT TTGAGAAAATTAGAAAAGCCCTTCCTAGTTCAGAAGACCTTGTAAAGTTAGCACCAGACTTTGACAAGATTGTTGAAAGCCTTAGCTTATTGAAGGACTT TTTTACCTCAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAGTGACAAGCATTTTAGAAAGGTGTCAGACAAAGAGAAAATT GACCAACTTCAGGAAGAACTTCTGCACACTCAGTTGAAGTATCAGAGAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGTATTGC AGAAAGATGACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTTGATTGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTATGATGCCAGTTA TAATACGCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATATTCCCA AGAGGATCTGGGGAGATGATGACACGTTCTCCAGTTAAGGTGACTCTGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTCTCGGGAGTTTG ATCTTACCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGAACTTCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAGACCAT ATCCTTAAATGTAAAAGGCCCTGGACTACAGAGGATGGTGCTTGTTGACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACACAAAG GAAACTATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATGCCATCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACGCAGTATTGTTA CAGACTTGGTCAGTCAAATGGACCCTCATGGAAGGAGAACCATATTCGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGCAGGAT TCAGCAGATAATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTATTTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAGCTATA AGAGAATATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAAGCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGCAGTAT CAGACTGCTTTTGGAAAATGGTACGAGAGTCTGTTGAACAACAGGCTGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGGAAGAATAACTA TCCTCGCCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCTAAAAATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAAAACAT TGGGAGGAAATCCTTCAACAATCTTTGTGGGAAAGAGTATCAACTCATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGGAACTT TTAACACCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACAACTTCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAATTTTC CCGCTTTATGACAGAACCGAAAGGGAAAGAGCATGATGACATATTTGATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGTGGAAT GACTTTGCGGAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGGAAGACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTATTTATTTTATGG AAGAGGCTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGAAAACATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAATCGGAC CCAAGAACAGTGTGTTCACAATGAAACCAAGAATGAATTGGAGAAGATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAATAACC ACAGTCCGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTTTTAAAAACAG CTCTAAACCATTGTAACCTTTGTCGAAGAGGTTTTTATTACTACCAAAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTTTGGCG TATACAGCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAACTTACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTATTGGAA GATTTTGCTGAAGATGGTGAGAAGAAGATTAAATTGCTTACTGGTAAACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGAAAAAC TTGATGCTTTCATTGAAGCTCTTCATCAGGAGAAATAAATTAAAATCGTACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACATCAACGTCTTT TGTCCAGCCTCTTTTTCTTCTGCTGTTCCACCTTTCTAAACATACAATAAAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGCTTTAACCATCA GCTGCCTCTCGAATGGAAGAACAGTGGTAATGGATTAACATCCTATTTTGTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTATGGCCATTAGGT TCAGTCCTTGAAGATAAGAAACTTGTTCTCTGTTTGTTGTCTTATTTGTGGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATGTTCAGTTTCTT TTCCAGAAATACAATGCTAGGTGTTTTGAAATAAAACTTATATAGCAATTGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCTGCTAAATCATT GTATGTGTCTGTAGTATTCTATTCCCAGAAACTATTTGACCATGATAATTCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAGAAGAACCCTTA AAACAGGTTAATTTGGATTGTAACGTTCAGTGAAAGAAATTTCAACCCTTCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAGCTTAC CTATTTGATTCAGTTGCTGTTTTCTCACTCTCTATATCCATTTGAAATTGATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTGTTAATAGTGGT TTTTCTCCTGTTGACAGAGCCACCGGATTATGACACAGGATGAGGAAGATTAAGGATAATCAATTGACTAATTTCATTTAGAATATTATCAAACATTTCA ACTAGGTATCAGAAAAAGGCTTTCTTTCATAAGACTATTTTAAATAGAAATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCTCAGCTGAATAA AGAAAAATTTAGTTCAATTTATTGCAATTTAATTACAATACTACCTTCACAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGGCTAAAGGACAT TCAAGCAAAGAAATGCTTTCTTTACTTAAAATGTCTATCTCATTTGCTGCCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGTGATGT TTTTGATTTTACAGTTTGCTAAATCTTATTTTCTTGGAGTTGCTTTTTGGTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACATCAATGCATGC TTCGTTGTGATCCCTCAAGATGTAACACTTGGTATGCTCGGTTGAGGATATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTTTGTTTTATACT GTTTGATAAGAAAAGTAGGTCCAGCCTTAAGCAGCACAGATGCGCTGGTAGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGACAGGAG TGAATAGAAAGGGAGGAGAGCTCTATTATGTTCTATACACAGATTAGGAGATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACAGGTTAATGCAT GTTAATGAATATTTTTGCAGTTGTAAAGCATAACAATTACAACTACACATCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACTTCTGTCAACTA TAGAAAGAAAGACCTTCAGCTGTATTTCCACAGATTTCTCCCAAGGAAAAGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTATAAGTTTTAAA AAGAGATGGAGGGAGATCTTCATTTCTTTGAGGAGATCAGTATTGTAACGTATGTGAATAGATGATAACAATTAATATTACTAAAAGTCCCACATGAGAG TCCTGACGCCCTCTCCATGCCCCACAGTAATGTGGCTTCTTTCATGGGTTTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGCTTTATTTTTCC TTGAAAGCTAGGTATTTATCAACTGCAGATGTTATTGAAAGAAAATAAAATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAAAGTCA GAAATGATTCTAATTTAAACAAAAAGATACTAAATATACAGAAGTTAAATTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAATTTGCAAAGAGT GGAGTGGACAAAGCTCTGTATGGAAGACTGAACAACTGTAAATAGATGATATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAATCAGTGTACCTA GGCAGTGCACAGCACGAAATAAGTGGCCCTTGCAGCTTCCCCGTTTAACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGTTTATG ATATTGCCCTCTTTGTATTCCCATTTTCTACAGTTTTTTCCGCAGACTTCTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGATATAAAAATAA GTAGGGAACATGGCAGAGAGTGGTGCTTCCCAGCCTCACAATGTGGGAATTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATCTTTAG TTAATAATTTTGTATTTATTTATTCTAGATGTATGTATCTGAGGAAAGAAATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTTTTTCT CTCAGTTCTCTAAGCTGGTCTATGTTATAGCTCTAGCAGTATGGAAATGTGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTATATGTTGTTGAG ATATTTGAAACTTACCTTGTTTTCACTTGTGCACTGTGAATGAACTTTGTATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTATTGCAACTTAT ATTTTGCCTGAGCTTGATCAAAGGTCATTTGTGTAGATGAGTAATTAAAAAATATTTAAATCAC

refseq_OPA1.F1 (forward primer)
OPA1.u.r.6 (reverse primer)
Single exon skipping

Frame 0

HGGSRVAVSAPA*PPRRRDVATTSGRCGL*GLP VFSETQLWNKRKFTTTKTTSGFTKHLSFTSSYLK ASTTPIKDILSAVLFSDKPSFT*TEILSN*IWL PASQEFLASKISYETLKTSLSHTRIGCWGWLHS QKDF*SVERYDTGP**I*MDCA*HCVGN**VYRF *EN*KSPS*FRRPCKVSTRL*QDC*KP*LIEGL FYLRFSGRNGV*SNRSWI*K*QAF*KGVRQREN *PTSGRTSAHSVEVSENLGTIRKGEQRIEKISIA ER*QRHSS*KA*EIFD*HVF*SS*CSL*L*CQL *YARSSATGCCGWRSECWKD*CVGNDCPSSNIP KRIWGDDDTFSS*GDSE*RSSPCGPI*R*FSGV* SYQRRRSCSIKT*NRTSNEEKCERRLYR*P*DH ILKCKRPWTTEDGAC*LTRCD*YCDIRHGS*HK GNYFQYQQSLHAES*CHHTVYSRWICGC*TQYCY RLGQSNGPSWKENHIRFDQSRPGREKCSQSKQD SADN*RKALPNESFRLFCCCNRKREQL*KH*SY KRI*RRVFSEFKAPKDKHAKGTPSDYKKFKPCSI RLLLENGTRVC*TTG**FQSNTF*P*N*MEE*L SSPAGT*PE*TI*KS*K*NP**SYQSEPGYTKT LGGNPSTIFVGKSINSCD*KHLPSSCADHEFRNF *HHSGYQA*TVD**TTS**SSRGCLGDPTRRIF PLYDRTERERA**HI**T*RGC*GRKY*TTQVE *LCGGQLEGYSTQCFGRPIHI**TAMGCSYLFYG RGSAGSSQGY*KCN*KHGGSRLEKEVVILEESD PRTVCSQ*NQE*IGEDVEM**GAPSLSCK**NN HSPEEP*IPRSRSRSKLD*GYLASSL*KTFFKNS SKPL*PLSKRFLLLPKAFCRF*VGMQ*CGLVLA YTAHACYHRKYFKATTYKY*S*AIREKC*RGIG RFC*RW*EED*IAYW*TRSTGGRPQES*RNSRKT *CFH*SSSSGEIN*NRTHNQLCIHLKNKNINVF CPASFSSAVPPF*TYNKVMG*K*SMYVTGALTI SCLSNGRTVVMD*HPILLY*SDKSE*YNWYGH*V QSLKIRNLFSVCCLICGGTRLMD*LRLLNVQFL FQKYNARCFEIKLI*QLFKVINCI*NHSSLLNH CMCL*YSIPRNYLTMIIQFIFTT*KKNG*QKNP* NRLIWIVTFSERNFNPS*PAKKFALEAKSVPAY LFDSVAVFSLSISI*N*FILDVVYLR*AFC**W FFSC*QSHRIMTQDEED*G*SID*FHLEYYQTFQ LGIRKRLSFIRLF*IEIISTIKVMLTIPLSAE* RKI*FNLLQFNYNTTFTTFSCVLNKYFLIG*RT FKQRNAFFT*NVYLICCLFTKPLLC**KCPLCDV FDFTVC*ILFSWSCFLVTAPLLLPILLFYINAC FVVIPQDVTLGMLG*GYEKILPKPGIQCMFVLY CLIRKVGPALSSTDALVDA*SGTFFISFRSRDRS E*KGRRALLCSIHRLGDDLTGYTPLTSAYRLMH VNEYFCSCKA*QLQLHIYF*RIKQDHIYLLLST IERKTFSCISTDFSQGKG*Y*SLLLSHPFV*VLK RDGGRSSFL*GDQYCNVCE*MITINITKSPT*E S*RPLHAPQ*CGFFHGFFFLLFS*SHPKHALFF LES*VFINCRCY*KKIKFSLKSKPCVLCL*FKSQ K*F*FKQKDTKYTEVKFELATESFVFMSEFAKS GVDKALYGRLNNCK*MISKLNLARTSILKISVP RQCTARNKWPLQLPRLTHCAIVAGGTVNLSSSL* YCPLCIPIFYSFFRRLLSANYSASKCK*MI*K* VGNMAESGASQPHNVGI*HRMRVRV*V*KIKSL VNNFVFIYSRCMYLRKEIWYFCFPIKGIKVMVFL SVL*AGLCYSSSSMEMCFKICLPFE*SWLYVVE IFETYLVFTCAL*MNFVLFF*NLHITCRYYCNL YFA*A*SKVICVDE*LKNI*I

Frame 1

TGAPAWPSRRLRDLPAGGMWRLRRAAVACEVCQ SLVKHSSGIKGSLPLQKLHLVSRSIYHSHHPTL KLQRPQLRTSFQQFSSLTNLPLRKLKFSPIKYGY QPRRNFWPARLATRLLKLRYLILGSAVGGGYTA KKTFDQWKDMIPDLSEYKWIVPDIVWEIDEYID FEKIRKALPSSEDLVKLAPDFDKIVESLSLLKDF FTSGSPEETAFRATDRGSESDKHFRKVSDKEKI DQLQEELLHTQLKYQRILERLEKENKELRKLVL QKDDKGIHHRKLKKSLIDMYSEVLDVLSDYDASY NTQDHLPRVVVVGDQSAGKTSVLEMIAQARIFP RGSGEMMTRSPVKVTLSEGPHHVALFKDSSREF DLTKEEDLAALRHEIELRMRKNVKEGCTVSPETI SLNVKGPGLQRMVLVDLPGVINTVTSGMAPDTK ETIFSISKAYMQNPNAIILCIQDGSVDAERSIV TDLVSQMDPHGRRTIFVLTKVDLAEKNVASPSRI QQIIEGKLFPMKALGYFAVVTGKGNSSESIEAI REYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAV SDCFWKMVRESVEQQADSFKATRFNLETEWKNNY PRLRELDRNELFEKAKNEILDEVISLSQVTPKH WEEILQQSLWERVSTHVIENIYLPAAQTMNSGT FNTTVDIKLKQWTDKQLPNKAVEVAWETLQEEFS RFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWN DFAEDSLRVIQHNALEDRSISDKQQWDAAIYFM EEALQARLKDTENAIENMVGPDWKKRWLYWKNRT QEQCVHNETKNELEKMLKCNEEHPAYLASDEIT TVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKT ALNHCNLCRRGFYYYQRHFVDSELECNDVVLFWR IQRMLAITANTLRQQLTNTEVRRLEKNVKEVLE DFAEDGEKKIKLLTGKRVQLAEDLKKVREIQEK LDAFIEALHQEK*IKIVLIISSAYI*RTKTSTSF VQPLFLLLFHLSKHTIKSWDKNNRCMLRAL*PS AASRMEEQW*WINILFCCTKVTNRNNIIGMAIR FSP*R*ETCSLFVVLFVVALV*WIN*GCSMFSFF SRNTMLGVLK*NLYSNCLKLSIVYKITVAC*II VCVCSILFPETI*P**FSLYSPHERKMGNRRTL KTG*FGL*RSVKEISTLHSQRRNLPWKPSQYQLT YLIQLLFSHSLYPFEIDLF*MLYTYVRLSVNSG FSPVDRATGL*HRMRKIKDNQLTNFI*NIIKHF N*VSEKGFLS*DYFK*KLFQQLK*C*PSPSQLNK EKFSSIYCNLITILPSQHFHVF*INIF*LAKGH SSKEMLSLLKMSISFAAFSLSLYFVNKSVHCVM FLILQFAKSYFLGVAFW*QPHCYSPFYCFTSMHA SL*SLKM*HLVCSVEDMKKYFRNQEFNVCLFYT V**EK*VQP*AAQMRW*MHSQELFLFLLGLGTG VNRKGGELYYVLYTD*EMTLLGTPL*PVLTG*CM LMNIFAVVKHNNYNYTSISKE*NRTIFIYFCQL *KERPSAVFPQISPKEKANISHYCYHIPLYKF* KEMEGDLHFFEEISIVTYVNR**QLILLKVPHES PDALSMPHSNVASFMGFFFFFLADLILSMLYFS LKARYLSTADVIERK*NSVSRVNPVSCVCSSKV RNDSNLNKKILNIQKLNSN*PQNHLFLCQNLQRV EWTKLCMED*TTVNR*YPNLIWLGLQF*KSVYL GSAQHEISGPCSFPV*PTVL*LRVEQSTFLVVY DIALFVFPFSTVFSADFFLQIIQPPNANE*YKNK *GTWQRVVLPSLTMWEFDIG*ESEYRFKR*NL* LIILYLFILDVCI*GKKSGIFAFQ*RGSK*WFF SQFSKLVYVIALAVWKCALKYAYLLNDHGYMLLR YLKLTLFSLVHCE*TLYYFFKTFTLRVDIIATY ILPELDQRSFV*MSN*KIFKS

Frame 2

RGLPRGRLGACVTSPPAGCGDYVGPLWPVRSAS L**NTALE*KEVYHYKNYIWFHEAFIIHIILP* SFNDPN*GHPFSSSLL*QTFLYVN*NSLQLNMA TSLAGIFGQQD*LRDS*NFAISY*DRLLGVATQP KRLLISGKI*YRTLVNINGLCLTLCGKLMSISI LRKLEKPFLVQKTL*S*HQTLTRLLKALAY*RT FLPQVLRKKRRLEQQIVDLKVTSILERCQTKRKL TNFRKNFCTLS*SIRESWND*KRRTKN*EN*YC RKMTKAFIIESLRNL*LTCILKFLMFSLIMMPV IIRKIICHGLLWLEIRVLERLVCWK*LPKLEYSQ EDLGR**HVLQLR*L*VKVLTMWPYLKIVLGSL ILPKKKILQH*DMK*NFE*GKM*KKAVPLALRP YP*M*KALDYRGWCLLTYQV*LIL*HQAWLLTQR KLFSVSAKLTCRILMPSYCVFKMDLWMLNAVLL QTWSVKWTLMEGEPYSF*PK*TWQRKM*PVQAG FSR*LKESSSQ*KL*VILLL*QEKGTALKALKL* ENMKKSFFRIQSS*RQAC*RHTK*LQEI*ALQY QTAFGKWYESLLNNRLIVSKQHVLTLKLNGRIT ILACGNLTGMNYLKKLKMKSLMKLSV*ARLHQNI GRKSFNNLCGKEYQLM*LKTSTFQLRRP*IQEL LTPQWISSLNSGLINNFLIKQ*RLLGRPYKKNF PAL*QNRKGKSMMTYLINLKRLLRKKVLNDTSGM TLRRTA*GLFNTMLWKTDPYLINSNGMQLFILW KRLCRLVSRILKMQLKTWWVQTGKRGGYTGRIG PKNSVFTMKPRMNWRRC*NVMRSTQLILQVMK*P QSGRTLNPEE*K*IQA*LRILGIKFIEDIF*KQ L*TIVTFVEEVFITTKGIL*ILSWNAMMWSCFG VYSACLLSPQIL*GNNLQILKLGD*RKMLKRYWK ILLKMVRRRLNCLLVNAFNWRKTSRKLEKFKKN LMLSLKLFIRRNKLKSYS*SALHTSEEQKHQRL LSSLFFFCCSTFLNIQ*SHGIKIIDVCYGRFNHQ LPLEWKNSGNGLTSYFVVLK*QIGII*LVWPLG SVLEDKKLVLCLLSYLWWHSFNGLTEVAQCSVS FPEIQC*VF*NKTYIAIV*SYQLYIKSQ*PAKSL YVSVVFYSQKLFDHDNSVYIHHMKEKWVTEEPL KQVNLDCNVQ*KKFQPFIASEEICLGSQVSTSL PI*FSCCFLTLYIHLKLIYFRCCILTLGFLLIVV FLLLTEPPDYDTG*GRLRIIN*LISFRILSNIS TRYQKKAFFHKTILNRNYFNN*SNVDHPPLS*I KKNLVQFIAI*LQYYLHNIFMCFK*IFFNWLKDI QAKKCFLYLKCLSHLLPFH*AFTLLIKVSIV*C F*FYSLLNLIFLELLFGNSPIATPHFIVLHQCM LRCDPSRCNTWYARLRI*KNTSETRNSMYVCFIL FDKKSRSSLKQHRCAGRCIVRNFFYFF*V*GQE *IEREESSIMFYTQIRR*PYWVHPSNQCLQVNA C**IFLQL*SITITTTHLFLKNKTGPYLFTSVNY RKKDLQLYFHRFLPRKRLILVTTVITSLCISFK KRWREIFISLRRSVL*RM*IDDNN*YY*KSHMR VLTPSPCPTVMWLLSWVFFSSF*LISS*ACFIFP *KLGIYQLQMLLKENKIQSQE*TLCLVSVVQKS EMILI*TKRY*IYRS*IRTSHRIICFYVRICKE WSGQSSVWKTEQL*IDDIQT*FG*DFNFKNQCT* AVHSTK*VALAASPFNPLCYSCGWNSQPF**FM ILPSLYSHFLQFFPQTSFCKLFSLQMQMNDIKI SREHGREWCFPASQCGNLT*DESQSIGLKDKIFS **FCIYLF*MYVSEERNLVFLLSNKGDQSNGFS LSSLSWSML*L*QYGNVL*NMLTF*MIMAICC* DI*NLPCFHLCTVNELCIIFLKPSHYV*ILLQLI FCLSLIKGHLCR*VIKKYLNH

Sequence NM_130836

CACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTGGCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAG TCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTGGTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAA AGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTCCTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTA CCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTATCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCC AAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATTGTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATT TTGAGAAAATTAGAAAAGCCCTTCCTAGTTCAGAAGACCTTGTAAAGTTAGCACCAGACTTTGACAAGATTGTTGAAAGCCTTAGCTTATTGAAGGACTT TTTTACCTCAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAGTGACAAGCATTTTAGAAAGGGTCTGCTTGGTGAGCTCATT CTCTTACAACAACAAATTCAAGAGCATGAAGAGGAAGCGCGCAGAGCCGCTGGCCAATATAGCACGAGCTATGCCCAACAGAAGCGCAAGGTGTCAGACA AAGAGAAAATTGACCAACTTCAGGAAGAACTTCTGCACACTCAGTTGAAGTATCAGAGAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAA ATTAGTATTGCAGAAAGATGACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTTGATTGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTAT GATGCCAGTTATAATACGCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTC GAATATTCCCAAGAGGATCTGGGGAGATGATGACACGTTCTCCAGTTAAGGTGACTCTGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTC TCGGGAGTTTGATCTTACCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGAACTTCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGC CCTGAGACCATATCCTTAAATGTAAAAGGCCCTGGACTACAGAGGATGGTGCTTGTTGACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTC CTGACACAAAGGAAACTATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATGCCATCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACG CAGTATTGTTACAGACTTGGTCAGTCAAATGGACCCTCATGGAAGGAGAACCATATTCGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGT CCAAGCAGGATTCAGCAGATAATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTATTTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCA TTGAAGCTATAAGAGAATATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAAGCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAG CCTTGCAGTATCAGACTGCTTTTGGAAAATGGTACGAGAGTCTGTTGAACAACAGGCTGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGG AAGAATAACTATCCTCGCCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCTAAAAATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTA CACCAAAACATTGGGAGGAAATCCTTCAACAATCTTTGTGGGAAAGAGTATCAACTCATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAA TTCAGGAACTTTTAACACCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACAACTTCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAA GAAGAATTTTCCCGCTTTATGACAGAACCGAAAGGGAAAGAGCATGATGACATATTTGATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGAC ACAAGTGGAATGACTTTGCGGAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGGAAGACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTAT TTATTTTATGGAAGAGGCTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGAAAACATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGG AAGAATCGGACCCAAGAACAGTGTGTTCACAATGAAACCAAGAATGAATTGGAGAAGATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTG ATGAAATAACCACAGTCCGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTT TTTAAAAACAGCTCTAAACCATTGTAACCTTTGTCGAAGAGGTTTTTATTACTACCAAAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTC TTGTTTTGGCGTATACAGCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAACTTACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAG AGGTATTGGAAGATTTTGCTGAAGATGGTGAGAAGAAGATTAAATTGCTTACTGGTAAACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAAT TCAAGAAAAACTTGATGCTTTCATTGAAGCTCTTCATCAGGAGAAATAAATTAAAATCGTACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACA TCAACGTCTTTTGTCCAGCCTCTTTTTCTTCTGCTGTTCCACCTTTCTAAACATACAATAAAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGC TTTAACCATCAGCTGCCTCTCGAATGGAAGAACAGTGGTAATGGATTAACATCCTATTTTGTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTAT GGCCATTAGGTTCAGTCCTTGAAGATAAGAAACTTGTTCTCTGTTTGTTGTCTTATTTGTGGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATG TTCAGTTTCTTTTCCAGAAATACAATGCTAGGTGTTTTGAAATAAAACTTATATAGCAATTGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCT GCTAAATCATTGTATGTGTCTGTAGTATTCTATTCCCAGAAACTATTTGACCATGATAATTCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAG AAGAACCCTTAAAACAGGTTAATTTGGATTGTAACGTTCAGTGAAAGAAATTTCAACCCTTCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAG TACCAGCTTACCTATTTGATTCAGTTGCTGTTTTCTCACTCTCTATATCCATTTGAAATTGATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTG TTAATAGTGGTTTTTCTCCTGTTGACAGAGCCACCGGATTATGACACAGGATGAGGAAGATTAAGGATAATCAATTGACTAATTTCATTTAGAATATTAT CAAACATTTCAACTAGGTATCAGAAAAAGGCTTTCTTTCATAAGACTATTTTAAATAGAAATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCT CAGCTGAATAAAGAAAAATTTAGTTCAATTTATTGCAATTTAATTACAATACTACCTTCACAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGG CTAAAGGACATTCAAGCAAAGAAATGCTTTCTTTACTTAAAATGTCTATCTCATTTGCTGCCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCA TTGTGTGATGTTTTTGATTTTACAGTTTGCTAAATCTTATTTTCTTGGAGTTGCTTTTTGGTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACA TCAATGCATGCTTCGTTGTGATCCCTCAAGATGTAACACTTGGTATGCTCGGTTGAGGATATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTT TGTTTTATACTGTTTGATAAGAAAAGTAGGTCCAGCCTTAAGCAGCACAGATGCGCTGGTAGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCT AGGGACAGGAGTGAATAGAAAGGGAGGAGAGCTCTATTATGTTCTATACACAGATTAGGAGATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACA GGTTAATGCATGTTAATGAATATTTTTGCAGTTGTAAAGCATAACAATTACAACTACACATCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACT TCTGTCAACTATAGAAAGAAAGACCTTCAGCTGTATTTCCACAGATTTCTCCCAAGGAAAAGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTA TAAGTTTTAAAAAGAGATGGAGGGAGATCTTCATTTCTTTGAGGAGATCAGTATTGTAACGTATGTGAATAGATGATAACAATTAATATTACTAAAAGTC CCACATGAGAGTCCTGACGCCCTCTCCATGCCCCACAGTAATGTGGCTTCTTTCATGGGTTTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGC TTTATTTTTCCTTGAAAGCTAGGTATTTATCAACTGCAGATGTTATTGAAAGAAAATAAAATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAG TTCAAAAGTCAGAAATGATTCTAATTTAAACAAAAAGATACTAAATATACAGAAGTTAAATTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAAT TTGCAAAGAGTGGAGTGGACAAAGCTCTGTATGGAAGACTGAACAACTGTAAATAGATGATATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAAT CAGTGTACCTAGGCAGTGCACAGCACGAAATAAGTGGCCCTTGCAGCTTCCCCGTTTAACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCT AGTAGTTTATGATATTGCCCTCTTTGTATTCCCATTTTCTACAGTTTTTTCCGCAGACTTCTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGA TATAAAAATAAGTAGGGAACATGGCAGAGAGTGGTGCTTCCCAGCCTCACAATGTGGGAATTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGAT AAAATCTTTAGTTAATAATTTTGTATTTATTTATTCTAGATGTATGTATCTGAGGAAAGAAATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTA ATGGTTTTTCTCTCAGTTCTCTAAGCTGGTCTATGTTATAGCTCTAGCAGTATGGAAATGTGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTAT ATGTTGTTGAGATATTTGAAACTTACCTTGTTTTCACTTGTGCACTGTGAATGAACTTTGTATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTA TTGCAACTTATATTTTGCCTGAGCTTGATCAAAGGTCATTTGTGTAGATGAGTAATTAAAAAATATTTAAATCAC

refseq_OPA1.F1 (forward primer)
OPA1.u.r.6 (reverse primer)
Single exon skipping

Frame 0

HGGSRVAVSAPA*PPRRRDVATTSGRCGL*GLP VFSETQLWNKRKFTTTKTTSGFTKHLSFTSSYLK ASTTPIKDILSAVLFSDKPSFT*TEILSN*IWL PASQEFLASKISYETLKTSLSHTRIGCWGWLHS QKDF*SVERYDTGP**I*MDCA*HCVGN**VYRF *EN*KSPS*FRRPCKVSTRL*QDC*KP*LIEGL FYLRFSGRNGV*SNRSWI*K*QAF*KGSAW*AH SLTTTNSRA*RGSAQSRWPI*HELCPTEAQGVRQ REN*PTSGRTSAHSVEVSENLGTIRKGEQRIEK ISIAER*QRHSS*KA*EIFD*HVF*SS*CSL*L *CQL*YARSSATGCCGWRSECWKD*CVGNDCPSS NIPKRIWGDDDTFSS*GDSE*RSSPCGPI*R*F SGV*SYQRRRSCSIKT*NRTSNEEKCERRLYR* P*DHILKCKRPWTTEDGAC*LTRCD*YCDIRHGS *HKGNYFQYQQSLHAES*CHHTVYSRWICGC*T QYCYRLGQSNGPSWKENHIRFDQSRPGREKCSQ SKQDSADN*RKALPNESFRLFCCCNRKREQL*KH *SYKRI*RRVFSEFKAPKDKHAKGTPSDYKKFK PCSIRLLLENGTRVC*TTG**FQSNTF*P*N*M EE*LSSPAGT*PE*TI*KS*K*NP**SYQSEPGY TKTLGGNPSTIFVGKSINSCD*KHLPSSCADHE FRNF*HHSGYQA*TVD**TTS**SSRGCLGDPT RRIFPLYDRTERERA**HI**T*RGC*GRKY*TT QVE*LCGGQLEGYSTQCFGRPIHI**TAMGCSY LFYGRGSAGSSQGY*KCN*KHGGSRLEKEVVIL EESDPRTVCSQ*NQE*IGEDVEM**GAPSLSCK* *NNHSPEEP*IPRSRSRSKLD*GYLASSL*KTF FKNSSKPL*PLSKRFLLLPKAFCRF*VGMQ*CG LVLAYTAHACYHRKYFKATTYKY*S*AIREKC*R GIGRFC*RW*EED*IAYW*TRSTGGRPQES*RN SRKT*CFH*SSSSGEIN*NRTHNQLCIHLKNKN INVFCPASFSSAVPPF*TYNKVMG*K*SMYVTGA LTISCLSNGRTVVMD*HPILLY*SDKSE*YNWY GH*VQSLKIRNLFSVCCLICGGTRLMD*LRLLN VQFLFQKYNARCFEIKLI*QLFKVINCI*NHSSL LNHCMCL*YSIPRNYLTMIIQFIFTT*KKNG*Q KNP*NRLIWIVTFSERNFNPS*PAKKFALEAKS VPAYLFDSVAVFSLSISI*N*FILDVVYLR*AFC **WFFSC*QSHRIMTQDEED*G*SID*FHLEYY QTFQLGIRKRLSFIRLF*IEIISTIKVMLTIPL SAE*RKI*FNLLQFNYNTTFTTFSCVLNKYFLIG *RTFKQRNAFFT*NVYLICCLFTKPLLC**KCP LCDVFDFTVC*ILFSWSCFLVTAPLLLPILLFY INACFVVIPQDVTLGMLG*GYEKILPKPGIQCMF VLYCLIRKVGPALSSTDALVDA*SGTFFISFRS RDRSE*KGRRALLCSIHRLGDDLTGYTPLTSAY RLMHVNEYFCSCKA*QLQLHIYF*RIKQDHIYLL LSTIERKTFSCISTDFSQGKG*Y*SLLLSHPFV *VLKRDGGRSSFL*GDQYCNVCE*MITINITKS PT*ES*RPLHAPQ*CGFFHGFFFLLFS*SHPKHA LFFLES*VFINCRCY*KKIKFSLKSKPCVLCL* FKSQK*F*FKQKDTKYTEVKFELATESFVFMSE FAKSGVDKALYGRLNNCK*MISKLNLARTSILKI SVPRQCTARNKWPLQLPRLTHCAIVAGGTVNLS SSL*YCPLCIPIFYSFFRRLLSANYSASKCK*M I*K*VGNMAESGASQPHNVGI*HRMRVRV*V*KI KSLVNNFVFIYSRCMYLRKEIWYFCFPIKGIKV MVFLSVL*AGLCYSSSSMEMCFKICLPFE*SWL YVVEIFETYLVFTCAL*MNFVLFF*NLHITCRYY CNLYFA*A*SKVICVDE*LKNI*I

Frame 1

TGAPAWPSRRLRDLPAGGMWRLRRAAVACEVCQ SLVKHSSGIKGSLPLQKLHLVSRSIYHSHHPTL KLQRPQLRTSFQQFSSLTNLPLRKLKFSPIKYGY QPRRNFWPARLATRLLKLRYLILGSAVGGGYTA KKTFDQWKDMIPDLSEYKWIVPDIVWEIDEYID FEKIRKALPSSEDLVKLAPDFDKIVESLSLLKDF FTSGSPEETAFRATDRGSESDKHFRKGLLGELI LLQQQIQEHEEEARRAAGQYSTSYAQQKRKVSD KEKIDQLQEELLHTQLKYQRILERLEKENKELRK LVLQKDDKGIHHRKLKKSLIDMYSEVLDVLSDY DASYNTQDHLPRVVVVGDQSAGKTSVLEMIAQA RIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSS REFDLTKEEDLAALRHEIELRMRKNVKEGCTVS PETISLNVKGPGLQRMVLVDLPGVINTVTSGMA PDTKETIFSISKAYMQNPNAIILCIQDGSVDAER SIVTDLVSQMDPHGRRTIFVLTKVDLAEKNVAS PSRIQQIIEGKLFPMKALGYFAVVTGKGNSSES IEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLS LAVSDCFWKMVRESVEQQADSFKATRFNLETEW KNNYPRLRELDRNELFEKAKNEILDEVISLSQV TPKHWEEILQQSLWERVSTHVIENIYLPAAQTMN SGTFNTTVDIKLKQWTDKQLPNKAVEVAWETLQ EEFSRFMTEPKGKEHDDIFDKLKEAVKEESIKR HKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAI YFMEEALQARLKDTENAIENMVGPDWKKRWLYW KNRTQEQCVHNETKNELEKMLKCNEEHPAYLAS DEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHF LKTALNHCNLCRRGFYYYQRHFVDSELECNDVV LFWRIQRMLAITANTLRQQLTNTEVRRLEKNVK EVLEDFAEDGEKKIKLLTGKRVQLAEDLKKVREI QEKLDAFIEALHQEK*IKIVLIISSAYI*RTKT STSFVQPLFLLLFHLSKHTIKSWDKNNRCMLRA L*PSAASRMEEQW*WINILFCCTKVTNRNNIIGM AIRFSP*R*ETCSLFVVLFVVALV*WIN*GCSM FSFFSRNTMLGVLK*NLYSNCLKLSIVYKITVA C*IIVCVCSILFPETI*P**FSLYSPHERKMGNR RTLKTG*FGL*RSVKEISTLHSQRRNLPWKPSQ YQLTYLIQLLFSHSLYPFEIDLF*MLYTYVRLS VNSGFSPVDRATGL*HRMRKIKDNQLTNFI*NII KHFN*VSEKGFLS*DYFK*KLFQQLK*C*PSPS QLNKEKFSSIYCNLITILPSQHFHVF*INIF*L AKGHSSKEMLSLLKMSISFAAFSLSLYFVNKSVH CVMFLILQFAKSYFLGVAFW*QPHCYSPFYCFT SMHASL*SLKM*HLVCSVEDMKKYFRNQEFNVC LFYTV**EK*VQP*AAQMRW*MHSQELFLFLLGL GTGVNRKGGELYYVLYTD*EMTLLGTPL*PVLT G*CMLMNIFAVVKHNNYNYTSISKE*NRTIFIY FCQL*KERPSAVFPQISPKEKANISHYCYHIPLY KF*KEMEGDLHFFEEISIVTYVNR**QLILLKV PHESPDALSMPHSNVASFMGFFFFFLADLILSM LYFSLKARYLSTADVIERK*NSVSRVNPVSCVCS SKVRNDSNLNKKILNIQKLNSN*PQNHLFLCQN LQRVEWTKLCMED*TTVNR*YPNLIWLGLQF*K SVYLGSAQHEISGPCSFPV*PTVL*LRVEQSTFL VVYDIALFVFPFSTVFSADFFLQIIQPPNANE* YKNK*GTWQRVVLPSLTMWEFDIG*ESEYRFKR *NL*LIILYLFILDVCI*GKKSGIFAFQ*RGSK* WFFSQFSKLVYVIALAVWKCALKYAYLLNDHGY MLLRYLKLTLFSLVHCE*TLYYFFKTFTLRVDI IATYILPELDQRSFV*MSN*KIFKS

Frame 2

RGLPRGRLGACVTSPPAGCGDYVGPLWPVRSAS L**NTALE*KEVYHYKNYIWFHEAFIIHIILP* SFNDPN*GHPFSSSLL*QTFLYVN*NSLQLNMA TSLAGIFGQQD*LRDS*NFAISY*DRLLGVATQP KRLLISGKI*YRTLVNINGLCLTLCGKLMSISI LRKLEKPFLVQKTL*S*HQTLTRLLKALAY*RT FLPQVLRKKRRLEQQIVDLKVTSILERVCLVSSF SYNNKFKSMKRKRAEPLANIARAMPNRSARCQT KRKLTNFRKNFCTLS*SIRESWND*KRRTKN*E N*YCRKMTKAFIIESLRNL*LTCILKFLMFSLIM MPVIIRKIICHGLLWLEIRVLERLVCWK*LPKL EYSQEDLGR**HVLQLR*L*VKVLTMWPYLKIV LGSLILPKKKILQH*DMK*NFE*GKM*KKAVPLA LRPYP*M*KALDYRGWCLLTYQV*LIL*HQAWL LTQRKLFSVSAKLTCRILMPSYCVFKMDLWMLN AVLLQTWSVKWTLMEGEPYSF*PK*TWQRKM*PV QAGFSR*LKESSSQ*KL*VILLL*QEKGTALKA LKL*ENMKKSFFRIQSS*RQAC*RHTK*LQEI* ALQYQTAFGKWYESLLNNRLIVSKQHVLTLKLNG RITILACGNLTGMNYLKKLKMKSLMKLSV*ARL HQNIGRKSFNNLCGKEYQLM*LKTSTFQLRRP* IQELLTPQWISSLNSGLINNFLIKQ*RLLGRPYK KNFPAL*QNRKGKSMMTYLINLKRLLRKKVLND TSGMTLRRTA*GLFNTMLWKTDPYLINSNGMQL FILWKRLCRLVSRILKMQLKTWWVQTGKRGGYTG RIGPKNSVFTMKPRMNWRRC*NVMRSTQLILQV MK*PQSGRTLNPEE*K*IQA*LRILGIKFIEDI F*KQL*TIVTFVEEVFITTKGIL*ILSWNAMMWS CFGVYSACLLSPQIL*GNNLQILKLGD*RKMLK RYWKILLKMVRRRLNCLLVNAFNWRKTSRKLEK FKKNLMLSLKLFIRRNKLKSYS*SALHTSEEQKH QRLLSSLFFFCCSTFLNIQ*SHGIKIIDVCYGR FNHQLPLEWKNSGNGLTSYFVVLK*QIGII*LV WPLGSVLEDKKLVLCLLSYLWWHSFNGLTEVAQC SVSFPEIQC*VF*NKTYIAIV*SYQLYIKSQ*P AKSLYVSVVFYSQKLFDHDNSVYIHHMKEKWVT EEPLKQVNLDCNVQ*KKFQPFIASEEICLGSQVS TSLPI*FSCCFLTLYIHLKLIYFRCCILTLGFL LIVVFLLLTEPPDYDTG*GRLRIIN*LISFRIL SNISTRYQKKAFFHKTILNRNYFNN*SNVDHPPL S*IKKNLVQFIAI*LQYYLHNIFMCFK*IFFNW LKDIQAKKCFLYLKCLSHLLPFH*AFTLLIKVS IV*CF*FYSLLNLIFLELLFGNSPIATPHFIVLH QCMLRCDPSRCNTWYARLRI*KNTSETRNSMYV CFILFDKKSRSSLKQHRCAGRCIVRNFFYFF*V *GQE*IEREESSIMFYTQIRR*PYWVHPSNQCLQ VNAC**IFLQL*SITITTTHLFLKNKTGPYLFT SVNYRKKDLQLYFHRFLPRKRLILVTTVITSLC ISFKKRWREIFISLRRSVL*RM*IDDNN*YY*KS HMRVLTPSPCPTVMWLLSWVFFSSF*LISS*AC FIFP*KLGIYQLQMLLKENKIQSQE*TLCLVSV VQKSEMILI*TKRY*IYRS*IRTSHRIICFYVRI CKEWSGQSSVWKTEQL*IDDIQT*FG*DFNFKN QCT*AVHSTK*VALAASPFNPLCYSCGWNSQPF **FMILPSLYSHFLQFFPQTSFCKLFSLQMQMND IKISREHGREWCFPASQCGNLT*DESQSIGLKD KIFS**FCIYLF*MYVSEERNLVFLLSNKGDQS NGFSLSSLSWSML*L*QYGNVL*NMLTF*MIMAI CC*DI*NLPCFHLCTVNELCIIFLKPSHYV*IL LQLIFCLSLIKGHLCR*VIKKYLNH

Sequence NM_130837

CACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTGGCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAG TCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTGGTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAA AGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTCCTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTA CCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTATCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCC AAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATTGTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATT TTGAGAAAATTAGAAAAGCCCTTCCTAGTTCAGAAGACCTTGTAAAGTTAGCACCAGACTTTGACAAGATTGTTGAAAGCCTTAGCTTATTGAAGGACTT TTTTACCTCAGGTCACAAATTGGTTAGTGAAGTCATAGGAGCTTCTGACCTACTTCTCTTGTTAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGAT CGTGGATCTGAAAGTGACAAGCATTTTAGAAAGGGTCTGCTTGGTGAGCTCATTCTCTTACAACAACAAATTCAAGAGCATGAAGAGGAAGCGCGCAGAG CCGCTGGCCAATATAGCACGAGCTATGCCCAACAGAAGCGCAAGGTGTCAGACAAAGAGAAAATTGACCAACTTCAGGAAGAACTTCTGCACACTCAGTT GAAGTATCAGAGAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGTATTGCAGAAAGATGACAAAGGCATTCATCATAGAAAGCTT AAGAAATCTTTGATTGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTATGATGCCAGTTATAATACGCAAGATCATCTGCCACGGGTTGTTGTGG TTGGAGATCAGAGTGCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATATTCCCAAGAGGATCTGGGGAGATGATGACACGTTCTCCAGT TAAGGTGACTCTGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTCTCGGGAGTTTGATCTTACCAAAGAAGAAGATCTTGCAGCATTAAGA CATGAAATAGAACTTCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAGACCATATCCTTAAATGTAAAAGGCCCTGGACTACAGAGGA TGGTGCTTGTTGACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACACAAAGGAAACTATTTTCAGTATCAGCAAAGCTTACATGCA GAATCCTAATGCCATCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACGCAGTATTGTTACAGACTTGGTCAGTCAAATGGACCCTCATGGAAGG AGAACCATATTCGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGCAGGATTCAGCAGATAATTGAAGGAAAGCTCTTCCCAATGA AAGCTTTAGGTTATTTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAGCTATAAGAGAATATGAAGAAGAGTTTTTTCAGAATTCAAA GCTCCTAAAGACAAGCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGCAGTATCAGACTGCTTTTGGAAAATGGTACGAGAGTCTGTT GAACAACAGGCTGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGGAAGAATAACTATCCTCGCCTGCGGGAACTTGACCGGAATGAACTAT TTGAAAAAGCTAAAAATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAAAACATTGGGAGGAAATCCTTCAACAATCTTTGTGGGAAAG AGTATCAACTCATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGGAACTTTTAACACCACAGTGGATATCAAGCTTAAACAGTGG ACTGATAAACAACTTCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAATTTTCCCGCTTTATGACAGAACCGAAAGGGAAAGAGCATG ATGACATATTTGATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGTGGAATGACTTTGCGGAGGACAGCTTGAGGGTTATTCAACA CAATGCTTTGGAAGACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTATTTATTTTATGGAAGAGGCTCTGCAGGCTCGTCTCAAGGATACTGAA AATGCAATTGAAAACATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAATCGGACCCAAGAACAGTGTGTTCACAATGAAACCAAGAATG AATTGGAGAAGATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAATAACCACAGTCCGGAAGAACCTTGAATCCCGAGGAGTAGA AGTAGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTTTTAAAAACAGCTCTAAACCATTGTAACCTTTGTCGAAGAGGTTTT TATTACTACCAAAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTTTGGCGTATACAGCGCATGCTTGCTATCACCGCAAATACTT TAAGGCAACAACTTACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTATTGGAAGATTTTGCTGAAGATGGTGAGAAGAAGATTAAATT GCTTACTGGTAAACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGAAAAACTTGATGCTTTCATTGAAGCTCTTCATCAGGAGAAA TAAATTAAAATCGTACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACATCAACGTCTTTTGTCCAGCCTCTTTTTCTTCTGCTGTTCCACCTTT CTAAACATACAATAAAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGCTTTAACCATCAGCTGCCTCTCGAATGGAAGAACAGTGGTAATGGAT TAACATCCTATTTTGTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTATGGCCATTAGGTTCAGTCCTTGAAGATAAGAAACTTGTTCTCTGTTT GTTGTCTTATTTGTGGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATGTTCAGTTTCTTTTCCAGAAATACAATGCTAGGTGTTTTGAAATAAA ACTTATATAGCAATTGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCTGCTAAATCATTGTATGTGTCTGTAGTATTCTATTCCCAGAAACTAT TTGACCATGATAATTCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAGAAGAACCCTTAAAACAGGTTAATTTGGATTGTAACGTTCAGTGAAA GAAATTTCAACCCTTCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAGCTTACCTATTTGATTCAGTTGCTGTTTTCTCACTCTCTAT ATCCATTTGAAATTGATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTGTTAATAGTGGTTTTTCTCCTGTTGACAGAGCCACCGGATTATGACA CAGGATGAGGAAGATTAAGGATAATCAATTGACTAATTTCATTTAGAATATTATCAAACATTTCAACTAGGTATCAGAAAAAGGCTTTCTTTCATAAGAC TATTTTAAATAGAAATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCTCAGCTGAATAAAGAAAAATTTAGTTCAATTTATTGCAATTTAATTA CAATACTACCTTCACAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGGCTAAAGGACATTCAAGCAAAGAAATGCTTTCTTTACTTAAAATGTC TATCTCATTTGCTGCCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGTGATGTTTTTGATTTTACAGTTTGCTAAATCTTATTTTCTT GGAGTTGCTTTTTGGTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACATCAATGCATGCTTCGTTGTGATCCCTCAAGATGTAACACTTGGTAT GCTCGGTTGAGGATATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTTTGTTTTATACTGTTTGATAAGAAAAGTAGGTCCAGCCTTAAGCAGC ACAGATGCGCTGGTAGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGACAGGAGTGAATAGAAAGGGAGGAGAGCTCTATTATGTTCTA TACACAGATTAGGAGATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACAGGTTAATGCATGTTAATGAATATTTTTGCAGTTGTAAAGCATAACA ATTACAACTACACATCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACTTCTGTCAACTATAGAAAGAAAGACCTTCAGCTGTATTTCCACAGAT TTCTCCCAAGGAAAAGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTATAAGTTTTAAAAAGAGATGGAGGGAGATCTTCATTTCTTTGAGGAG ATCAGTATTGTAACGTATGTGAATAGATGATAACAATTAATATTACTAAAAGTCCCACATGAGAGTCCTGACGCCCTCTCCATGCCCCACAGTAATGTGG CTTCTTTCATGGGTTTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGCTTTATTTTTCCTTGAAAGCTAGGTATTTATCAACTGCAGATGTTAT TGAAAGAAAATAAAATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAAAGTCAGAAATGATTCTAATTTAAACAAAAAGATACTAAAT ATACAGAAGTTAAATTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAATTTGCAAAGAGTGGAGTGGACAAAGCTCTGTATGGAAGACTGAACAA CTGTAAATAGATGATATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAATCAGTGTACCTAGGCAGTGCACAGCACGAAATAAGTGGCCCTTGCAG CTTCCCCGTTTAACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGTTTATGATATTGCCCTCTTTGTATTCCCATTTTCTACAGTT TTTTCCGCAGACTTCTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGATATAAAAATAAGTAGGGAACATGGCAGAGAGTGGTGCTTCCCAGCC TCACAATGTGGGAATTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATCTTTAGTTAATAATTTTGTATTTATTTATTCTAGATGTATG TATCTGAGGAAAGAAATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTTTTTCTCTCAGTTCTCTAAGCTGGTCTATGTTATAGCTCTA GCAGTATGGAAATGTGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTATATGTTGTTGAGATATTTGAAACTTACCTTGTTTTCACTTGTGCACT GTGAATGAACTTTGTATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTATTGCAACTTATATTTTGCCTGAGCTTGATCAAAGGTCATTTGTGTA GATGAGTAATTAAAAAATATTTAAATCAC

refseq_OPA1.F1 (forward primer)
OPA1.u.r.6 (reverse primer)
Single exon skipping

Frame 0

HGGSRVAVSAPA*PPRRRDVATTSGRCGL*GLP VFSETQLWNKRKFTTTKTTSGFTKHLSFTSSYLK ASTTPIKDILSAVLFSDKPSFT*TEILSN*IWL PASQEFLASKISYETLKTSLSHTRIGCWGWLHS QKDF*SVERYDTGP**I*MDCA*HCVGN**VYRF *EN*KSPS*FRRPCKVSTRL*QDC*KP*LIEGL FYLRSQIG**SHRSF*PTSLVRFSGRNGV*SNR SWI*K*QAF*KGSAW*AHSLTTTNSRA*RGSAQS RWPI*HELCPTEAQGVRQREN*PTSGRTSAHSV EVSENLGTIRKGEQRIEKISIAER*QRHSS*KA *EIFD*HVF*SS*CSL*L*CQL*YARSSATGCCG WRSECWKD*CVGNDCPSSNIPKRIWGDDDTFSS *GDSE*RSSPCGPI*R*FSGV*SYQRRRSCSIK T*NRTSNEEKCERRLYR*P*DHILKCKRPWTTED GAC*LTRCD*YCDIRHGS*HKGNYFQYQQSLHA ES*CHHTVYSRWICGC*TQYCYRLGQSNGPSWK ENHIRFDQSRPGREKCSQSKQDSADN*RKALPNE SFRLFCCCNRKREQL*KH*SYKRI*RRVFSEFK APKDKHAKGTPSDYKKFKPCSIRLLLENGTRVC *TTG**FQSNTF*P*N*MEE*LSSPAGT*PE*TI *KS*K*NP**SYQSEPGYTKTLGGNPSTIFVGK SINSCD*KHLPSSCADHEFRNF*HHSGYQA*TV D**TTS**SSRGCLGDPTRRIFPLYDRTERERA* *HI**T*RGC*GRKY*TTQVE*LCGGQLEGYST QCFGRPIHI**TAMGCSYLFYGRGSAGSSQGY* KCN*KHGGSRLEKEVVILEESDPRTVCSQ*NQE* IGEDVEM**GAPSLSCK**NNHSPEEP*IPRSR SRSKLD*GYLASSL*KTFFKNSSKPL*PLSKRF LLLPKAFCRF*VGMQ*CGLVLAYTAHACYHRKYF KATTYKY*S*AIREKC*RGIGRFC*RW*EED*I AYW*TRSTGGRPQES*RNSRKT*CFH*SSSSGE IN*NRTHNQLCIHLKNKNINVFCPASFSSAVPPF *TYNKVMG*K*SMYVTGALTISCLSNGRTVVMD *HPILLY*SDKSE*YNWYGH*VQSLKIRNLFSV CCLICGGTRLMD*LRLLNVQFLFQKYNARCFEIK LI*QLFKVINCI*NHSSLLNHCMCL*YSIPRNY LTMIIQFIFTT*KKNG*QKNP*NRLIWIVTFSE RNFNPS*PAKKFALEAKSVPAYLFDSVAVFSLSI SI*N*FILDVVYLR*AFC**WFFSC*QSHRIMT QDEED*G*SID*FHLEYYQTFQLGIRKRLSFIR LF*IEIISTIKVMLTIPLSAE*RKI*FNLLQFNY NTTFTTFSCVLNKYFLIG*RTFKQRNAFFT*NV YLICCLFTKPLLC**KCPLCDVFDFTVC*ILFS WSCFLVTAPLLLPILLFYINACFVVIPQDVTLGM LG*GYEKILPKPGIQCMFVLYCLIRKVGPALSS TDALVDA*SGTFFISFRSRDRSE*KGRRALLCS IHRLGDDLTGYTPLTSAYRLMHVNEYFCSCKA*Q LQLHIYF*RIKQDHIYLLLSTIERKTFSCISTD FSQGKG*Y*SLLLSHPFV*VLKRDGGRSSFL*G DQYCNVCE*MITINITKSPT*ES*RPLHAPQ*CG FFHGFFFLLFS*SHPKHALFFLES*VFINCRCY *KKIKFSLKSKPCVLCL*FKSQK*F*FKQKDTK YTEVKFELATESFVFMSEFAKSGVDKALYGRLNN CK*MISKLNLARTSILKISVPRQCTARNKWPLQ LPRLTHCAIVAGGTVNLSSSL*YCPLCIPIFYS FFRRLLSANYSASKCK*MI*K*VGNMAESGASQP HNVGI*HRMRVRV*V*KIKSLVNNFVFIYSRCM YLRKEIWYFCFPIKGIKVMVFLSVL*AGLCYSS SSMEMCFKICLPFE*SWLYVVEIFETYLVFTCAL *MNFVLFF*NLHITCRYYCNLYFA*A*SKVICV DE*LKNI*I

Frame 1

TGAPAWPSRRLRDLPAGGMWRLRRAAVACEVCQ SLVKHSSGIKGSLPLQKLHLVSRSIYHSHHPTL KLQRPQLRTSFQQFSSLTNLPLRKLKFSPIKYGY QPRRNFWPARLATRLLKLRYLILGSAVGGGYTA KKTFDQWKDMIPDLSEYKWIVPDIVWEIDEYID FEKIRKALPSSEDLVKLAPDFDKIVESLSLLKDF FTSGHKLVSEVIGASDLLLLLGSPEETAFRATD RGSESDKHFRKGLLGELILLQQQIQEHEEEARR AAGQYSTSYAQQKRKVSDKEKIDQLQEELLHTQL KYQRILERLEKENKELRKLVLQKDDKGIHHRKL KKSLIDMYSEVLDVLSDYDASYNTQDHLPRVVV VGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPV KVTLSEGPHHVALFKDSSREFDLTKEEDLAALR HEIELRMRKNVKEGCTVSPETISLNVKGPGLQR MVLVDLPGVINTVTSGMAPDTKETIFSISKAYMQ NPNAIILCIQDGSVDAERSIVTDLVSQMDPHGR RTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPM KALGYFAVVTGKGNSSESIEAIREYEEEFFQNSK LLKTSMLKAHQVTTRNLSLAVSDCFWKMVRESV EQQADSFKATRFNLETEWKNNYPRLRELDRNEL FEKAKNEILDEVISLSQVTPKHWEEILQQSLWER VSTHVIENIYLPAAQTMNSGTFNTTVDIKLKQW TDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEH DDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQH NALEDRSISDKQQWDAAIYFMEEALQARLKDTE NAIENMVGPDWKKRWLYWKNRTQEQCVHNETKN ELEKMLKCNEEHPAYLASDEITTVRKNLESRGVE VDPSLIKDTWHQVYRRHFLKTALNHCNLCRRGF YYYQRHFVDSELECNDVVLFWRIQRMLAITANT LRQQLTNTEVRRLEKNVKEVLEDFAEDGEKKIKL LTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK *IKIVLIISSAYI*RTKTSTSFVQPLFLLLFHL SKHTIKSWDKNNRCMLRAL*PSAASRMEEQW*WI NILFCCTKVTNRNNIIGMAIRFSP*R*ETCSLF VVLFVVALV*WIN*GCSMFSFFSRNTMLGVLK* NLYSNCLKLSIVYKITVAC*IIVCVCSILFPETI *P**FSLYSPHERKMGNRRTLKTG*FGL*RSVK EISTLHSQRRNLPWKPSQYQLTYLIQLLFSHSL YPFEIDLF*MLYTYVRLSVNSGFSPVDRATGL*H RMRKIKDNQLTNFI*NIIKHFN*VSEKGFLS*D YFK*KLFQQLK*C*PSPSQLNKEKFSSIYCNLI TILPSQHFHVF*INIF*LAKGHSSKEMLSLLKMS ISFAAFSLSLYFVNKSVHCVMFLILQFAKSYFL GVAFW*QPHCYSPFYCFTSMHASL*SLKM*HLV CSVEDMKKYFRNQEFNVCLFYTV**EK*VQP*AA QMRW*MHSQELFLFLLGLGTGVNRKGGELYYVL YTD*EMTLLGTPL*PVLTG*CMLMNIFAVVKHN NYNYTSISKE*NRTIFIYFCQL*KERPSAVFPQI SPKEKANISHYCYHIPLYKF*KEMEGDLHFFEE ISIVTYVNR**QLILLKVPHESPDALSMPHSNV ASFMGFFFFFLADLILSMLYFSLKARYLSTADVI ERK*NSVSRVNPVSCVCSSKVRNDSNLNKKILN IQKLNSN*PQNHLFLCQNLQRVEWTKLCMED*T TVNR*YPNLIWLGLQF*KSVYLGSAQHEISGPCS FPV*PTVL*LRVEQSTFLVVYDIALFVFPFSTV FSADFFLQIIQPPNANE*YKNK*GTWQRVVLPS LTMWEFDIG*ESEYRFKR*NL*LIILYLFILDVC I*GKKSGIFAFQ*RGSK*WFFSQFSKLVYVIAL AVWKCALKYAYLLNDHGYMLLRYLKLTLFSLVH CE*TLYYFFKTFTLRVDIIATYILPELDQRSFV* MSN*KIFKS

Frame 2

RGLPRGRLGACVTSPPAGCGDYVGPLWPVRSAS L**NTALE*KEVYHYKNYIWFHEAFIIHIILP* SFNDPN*GHPFSSSLL*QTFLYVN*NSLQLNMA TSLAGIFGQQD*LRDS*NFAISY*DRLLGVATQP KRLLISGKI*YRTLVNINGLCLTLCGKLMSISI LRKLEKPFLVQKTL*S*HQTLTRLLKALAY*RT FLPQVTNWLVKS*ELLTYFSC*VLRKKRRLEQQI VDLKVTSILERVCLVSSFSYNNKFKSMKRKRAE PLANIARAMPNRSARCQTKRKLTNFRKNFCTLS *SIRESWND*KRRTKN*EN*YCRKMTKAFIIESL RNL*LTCILKFLMFSLIMMPVIIRKIICHGLLW LEIRVLERLVCWK*LPKLEYSQEDLGR**HVLQ LR*L*VKVLTMWPYLKIVLGSLILPKKKILQH*D MK*NFE*GKM*KKAVPLALRPYP*M*KALDYRG WCLLTYQV*LIL*HQAWLLTQRKLFSVSAKLTC RILMPSYCVFKMDLWMLNAVLLQTWSVKWTLMEG EPYSF*PK*TWQRKM*PVQAGFSR*LKESSSQ* KL*VILLL*QEKGTALKALKL*ENMKKSFFRIQ SS*RQAC*RHTK*LQEI*ALQYQTAFGKWYESLL NNRLIVSKQHVLTLKLNGRITILACGNLTGMNY LKKLKMKSLMKLSV*ARLHQNIGRKSFNNLCGK EYQLM*LKTSTFQLRRP*IQELLTPQWISSLNSG LINNFLIKQ*RLLGRPYKKNFPAL*QNRKGKSM MTYLINLKRLLRKKVLNDTSGMTLRRTA*GLFN TMLWKTDPYLINSNGMQLFILWKRLCRLVSRILK MQLKTWWVQTGKRGGYTGRIGPKNSVFTMKPRM NWRRC*NVMRSTQLILQVMK*PQSGRTLNPEE* K*IQA*LRILGIKFIEDIF*KQL*TIVTFVEEVF ITTKGIL*ILSWNAMMWSCFGVYSACLLSPQIL *GNNLQILKLGD*RKMLKRYWKILLKMVRRRLN CLLVNAFNWRKTSRKLEKFKKNLMLSLKLFIRRN KLKSYS*SALHTSEEQKHQRLLSSLFFFCCSTF LNIQ*SHGIKIIDVCYGRFNHQLPLEWKNSGNG LTSYFVVLK*QIGII*LVWPLGSVLEDKKLVLCL LSYLWWHSFNGLTEVAQCSVSFPEIQC*VF*NK TYIAIV*SYQLYIKSQ*PAKSLYVSVVFYSQKL FDHDNSVYIHHMKEKWVTEEPLKQVNLDCNVQ*K KFQPFIASEEICLGSQVSTSLPI*FSCCFLTLY IHLKLIYFRCCILTLGFLLIVVFLLLTEPPDYD TG*GRLRIIN*LISFRILSNISTRYQKKAFFHKT ILNRNYFNN*SNVDHPPLS*IKKNLVQFIAI*L QYYLHNIFMCFK*IFFNWLKDIQAKKCFLYLKC LSHLLPFH*AFTLLIKVSIV*CF*FYSLLNLIFL ELLFGNSPIATPHFIVLHQCMLRCDPSRCNTWY ARLRI*KNTSETRNSMYVCFILFDKKSRSSLKQ HRCAGRCIVRNFFYFF*V*GQE*IEREESSIMFY TQIRR*PYWVHPSNQCLQVNAC**IFLQL*SIT ITTTHLFLKNKTGPYLFTSVNYRKKDLQLYFHR FLPRKRLILVTTVITSLCISFKKRWREIFISLRR SVL*RM*IDDNN*YY*KSHMRVLTPSPCPTVMW LLSWVFFSSF*LISS*ACFIFP*KLGIYQLQML LKENKIQSQE*TLCLVSVVQKSEMILI*TKRY*I YRS*IRTSHRIICFYVRICKEWSGQSSVWKTEQ L*IDDIQT*FG*DFNFKNQCT*AVHSTK*VALA ASPFNPLCYSCGWNSQPF**FMILPSLYSHFLQF FPQTSFCKLFSLQMQMNDIKISREHGREWCFPA SQCGNLT*DESQSIGLKDKIFS**FCIYLF*MY VSEERNLVFLLSNKGDQSNGFSLSSLSWSML*L* QYGNVL*NMLTF*MIMAICC*DI*NLPCFHLCT VNELCIIFLKPSHYV*ILLQLIFCLSLIKGHLC R*VIKKYLNH

Sequence NM_130834

CACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGTGGCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAG TCTTTAGTGAAACACAGCTCTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTGGTTTCACGAAGCATTTATCATTCACATCATCCTACCTTAA AGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTCCTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCTA CCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCTATCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCC AAAAAGACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATTGTGCCTGACATTGTGTGGGAAATTGATGAGTATATCGATT TTGAGAAAATTAGAAAAGCCCTTCCTAGTTCAGAAGACCTTGTAAAGTTAGCACCAGACTTTGACAAGATTGTTGAAAGCCTTAGCTTATTGAAGGACTT TTTTACCTCAGGTCACAAATTGGTTAGTGAAGTCATAGGAGCTTCTGACCTACTTCTCTTGTTAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGAT CGTGGATCTGAAAGTGACAAGCATTTTAGAAAGGTGTCAGACAAAGAGAAAATTGACCAACTTCAGGAAGAACTTCTGCACACTCAGTTGAAGTATCAGA GAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGTATTGCAGAAAGATGACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTT GATTGACATGTATTCTGAAGTTCTTGATGTTCTCTCTGATTATGATGCCAGTTATAATACGCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAG AGTGCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATATTCCCAAGAGGATCTGGGGAGATGATGACACGTTCTCCAGTTAAGGTGACTC TGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTCTCGGGAGTTTGATCTTACCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGA ACTTCGAATGAGGAAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAGACCATATCCTTAAATGTAAAAGGCCCTGGACTACAGAGGATGGTGCTTGTT GACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACACAAAGGAAACTATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATG CCATCATACTGTGTATTCAAGATGGATCTGTGGATGCTGAACGCAGTATTGTTACAGACTTGGTCAGTCAAATGGACCCTCATGGAAGGAGAACCATATT CGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGCAGGATTCAGCAGATAATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGT TATTTTGCTGTTGTAACAGGAAAAGGGAACAGCTCTGAAAGCATTGAAGCTATAAGAGAATATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGA CAAGCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGCAGTATCAGACTGCTTTTGGAAAATGGTACGAGAGTCTGTTGAACAACAGGC TGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGGAAGAATAACTATCCTCGCCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCT AAAAATGAAATCCTTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAAAACATTGGGAGGAAATCCTTCAACAATCTTTGTGGGAAAGAGTATCAACTC ATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGGAACTTTTAACACCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACA ACTTCCTAATAAAGCAGTAGAGGTTGCTTGGGAGACCCTACAAGAAGAATTTTCCCGCTTTATGACAGAACCGAAAGGGAAAGAGCATGATGACATATTT GATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGTGGAATGACTTTGCGGAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGG AAGACCGATCCATATCTGATAAACAGCAATGGGATGCAGCTATTTATTTTATGGAAGAGGCTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGA AAACATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAATCGGACCCAAGAACAGTGTGTTCACAATGAAACCAAGAATGAATTGGAGAAG ATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTGATGAAATAACCACAGTCCGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAA GCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTTTTAAAAACAGCTCTAAACCATTGTAACCTTTGTCGAAGAGGTTTTTATTACTACCA AAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTTTGGCGTATACAGCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAA CTTACAAATACTGAAGTTAGGCGATTAGAGAAAAATGTTAAAGAGGTATTGGAAGATTTTGCTGAAGATGGTGAGAAGAAGATTAAATTGCTTACTGGTA AACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGAAAAACTTGATGCTTTCATTGAAGCTCTTCATCAGGAGAAATAAATTAAAAT CGTACTCATAATCAGCTCTGCATACATCTGAAGAACAAAAACATCAACGTCTTTTGTCCAGCCTCTTTTTCTTCTGCTGTTCCACCTTTCTAAACATACA ATAAAGTCATGGGATAAAAATAATCGATGTATGTTACGGGCGCTTTAACCATCAGCTGCCTCTCGAATGGAAGAACAGTGGTAATGGATTAACATCCTAT TTTGTTGTACTAAAGTGACAAATCGGAATAATATAATTGGTATGGCCATTAGGTTCAGTCCTTGAAGATAAGAAACTTGTTCTCTGTTTGTTGTCTTATT TGTGGTGGCACTCGTTTAATGGATTAACTGAGGTTGCTCAATGTTCAGTTTCTTTTCCAGAAATACAATGCTAGGTGTTTTGAAATAAAACTTATATAGC AATTGTTTAAAGTTATCAATTGTATATAAAATCACAGTAGCCTGCTAAATCATTGTATGTGTCTGTAGTATTCTATTCCCAGAAACTATTTGACCATGAT AATTCAGTTTATATTCACCACATGAAAGAAAAATGGGTAACAGAAGAACCCTTAAAACAGGTTAATTTGGATTGTAACGTTCAGTGAAAGAAATTTCAAC CCTTCATAGCCAGCGAAGAAATTTGCCTTGGAAGCCAAGTCAGTACCAGCTTACCTATTTGATTCAGTTGCTGTTTTCTCACTCTCTATATCCATTTGAA ATTGATTTATTTTAGATGTTGTATACTTACGTTAGGCTTTCTGTTAATAGTGGTTTTTCTCCTGTTGACAGAGCCACCGGATTATGACACAGGATGAGGA AGATTAAGGATAATCAATTGACTAATTTCATTTAGAATATTATCAAACATTTCAACTAGGTATCAGAAAAAGGCTTTCTTTCATAAGACTATTTTAAATA GAAATTATTTCAACAATTAAAGTAATGTTGACCATCCCCCTCTCAGCTGAATAAAGAAAAATTTAGTTCAATTTATTGCAATTTAATTACAATACTACCT TCACAACATTTTCATGTGTTTTAAATAAATATTTTTTAATTGGCTAAAGGACATTCAAGCAAAGAAATGCTTTCTTTACTTAAAATGTCTATCTCATTTG CTGCCTTTTCACTAAGCCTTTACTTTGTTAATAAAAGTGTCCATTGTGTGATGTTTTTGATTTTACAGTTTGCTAAATCTTATTTTCTTGGAGTTGCTTT TTGGTAACAGCCCCATTGCTACTCCCCATTTTATTGTTTTACATCAATGCATGCTTCGTTGTGATCCCTCAAGATGTAACACTTGGTATGCTCGGTTGAG GATATGAAAAAATACTTCCGAAACCAGGAATTCAATGTATGTTTGTTTTATACTGTTTGATAAGAAAAGTAGGTCCAGCCTTAAGCAGCACAGATGCGCT GGTAGATGCATAGTCAGGAACTTTTTTTATTTCTTTTAGGTCTAGGGACAGGAGTGAATAGAAAGGGAGGAGAGCTCTATTATGTTCTATACACAGATTA GGAGATGACCTTACTGGGTACACCCCTCTAACCAGTGCTTACAGGTTAATGCATGTTAATGAATATTTTTGCAGTTGTAAAGCATAACAATTACAACTAC ACATCTATTTCTAAAGAATAAAACAGGACCATATTTATTTACTTCTGTCAACTATAGAAAGAAAGACCTTCAGCTGTATTTCCACAGATTTCTCCCAAGG AAAAGGCTAATATTAGTCACTACTGTTATCACATCCCTTTGTATAAGTTTTAAAAAGAGATGGAGGGAGATCTTCATTTCTTTGAGGAGATCAGTATTGT AACGTATGTGAATAGATGATAACAATTAATATTACTAAAAGTCCCACATGAGAGTCCTGACGCCCTCTCCATGCCCCACAGTAATGTGGCTTCTTTCATG GGTTTTTTTTTCTTCTTTTTAGCTGATCTCATCCTAAGCATGCTTTATTTTTCCTTGAAAGCTAGGTATTTATCAACTGCAGATGTTATTGAAAGAAAAT AAAATTCAGTCTCAAGAGTAAACCCTGTGTCTTGTGTCTGTAGTTCAAAAGTCAGAAATGATTCTAATTTAAACAAAAAGATACTAAATATACAGAAGTT AAATTCGAACTAGCCACAGAATCATTTGTTTTTATGTCAGAATTTGCAAAGAGTGGAGTGGACAAAGCTCTGTATGGAAGACTGAACAACTGTAAATAGA TGATATCCAAACTTAATTTGGCTAGGACTTCAATTTTAAAAATCAGTGTACCTAGGCAGTGCACAGCACGAAATAAGTGGCCCTTGCAGCTTCCCCGTTT AACCCACTGTGCTATAGTTGCGGGTGGAACAGTCAACCTTTCTAGTAGTTTATGATATTGCCCTCTTTGTATTCCCATTTTCTACAGTTTTTTCCGCAGA CTTCTTTCTGCAAATTATTCAGCCTCCAAATGCAAATGAATGATATAAAAATAAGTAGGGAACATGGCAGAGAGTGGTGCTTCCCAGCCTCACAATGTGG GAATTTGACATAGGATGAGAGTCAGAGTATAGGTTTAAAAGATAAAATCTTTAGTTAATAATTTTGTATTTATTTATTCTAGATGTATGTATCTGAGGAA AGAAATCTGGTATTTTTGCTTTCCAATAAAGGGGATCAAAGTAATGGTTTTTCTCTCAGTTCTCTAAGCTGGTCTATGTTATAGCTCTAGCAGTATGGAA ATGTGCTTTAAAATATGCTTACCTTTTGAATGATCATGGCTATATGTTGTTGAGATATTTGAAACTTACCTTGTTTTCACTTGTGCACTGTGAATGAACT TTGTATTATTTTTTTAAAACCTTCACATTACGTGTAGATATTATTGCAACTTATATTTTGCCTGAGCTTGATCAAAGGTCATTTGTGTAGATGAGTAATT AAAAAATATTTAAATCAC

refseq_OPA1.F1 (forward primer)
OPA1.u.r.6 (reverse primer)
Single exon skipping

Frame 0

HGGSRVAVSAPA*PPRRRDVATTSGRCGL*GLP VFSETQLWNKRKFTTTKTTSGFTKHLSFTSSYLK ASTTPIKDILSAVLFSDKPSFT*TEILSN*IWL PASQEFLASKISYETLKTSLSHTRIGCWGWLHS QKDF*SVERYDTGP**I*MDCA*HCVGN**VYRF *EN*KSPS*FRRPCKVSTRL*QDC*KP*LIEGL FYLRSQIG**SHRSF*PTSLVRFSGRNGV*SNR SWI*K*QAF*KGVRQREN*PTSGRTSAHSVEVSE NLGTIRKGEQRIEKISIAER*QRHSS*KA*EIF D*HVF*SS*CSL*L*CQL*YARSSATGCCGWRS ECWKD*CVGNDCPSSNIPKRIWGDDDTFSS*GDS E*RSSPCGPI*R*FSGV*SYQRRRSCSIKT*NR TSNEEKCERRLYR*P*DHILKCKRPWTTEDGAC *LTRCD*YCDIRHGS*HKGNYFQYQQSLHAES*C HHTVYSRWICGC*TQYCYRLGQSNGPSWKENHI RFDQSRPGREKCSQSKQDSADN*RKALPNESFR LFCCCNRKREQL*KH*SYKRI*RRVFSEFKAPKD KHAKGTPSDYKKFKPCSIRLLLENGTRVC*TTG **FQSNTF*P*N*MEE*LSSPAGT*PE*TI*KS *K*NP**SYQSEPGYTKTLGGNPSTIFVGKSINS CD*KHLPSSCADHEFRNF*HHSGYQA*TVD**T TS**SSRGCLGDPTRRIFPLYDRTERERA**HI **T*RGC*GRKY*TTQVE*LCGGQLEGYSTQCFG RPIHI**TAMGCSYLFYGRGSAGSSQGY*KCN* KHGGSRLEKEVVILEESDPRTVCSQ*NQE*IGE DVEM**GAPSLSCK**NNHSPEEP*IPRSRSRSK LD*GYLASSL*KTFFKNSSKPL*PLSKRFLLLP KAFCRF*VGMQ*CGLVLAYTAHACYHRKYFKAT TYKY*S*AIREKC*RGIGRFC*RW*EED*IAYW* TRSTGGRPQES*RNSRKT*CFH*SSSSGEIN*N RTHNQLCIHLKNKNINVFCPASFSSAVPPF*TY NKVMG*K*SMYVTGALTISCLSNGRTVVMD*HPI LLY*SDKSE*YNWYGH*VQSLKIRNLFSVCCLI CGGTRLMD*LRLLNVQFLFQKYNARCFEIKLI* QLFKVINCI*NHSSLLNHCMCL*YSIPRNYLTMI IQFIFTT*KKNG*QKNP*NRLIWIVTFSERNFN PS*PAKKFALEAKSVPAYLFDSVAVFSLSISI* N*FILDVVYLR*AFC**WFFSC*QSHRIMTQDEE D*G*SID*FHLEYYQTFQLGIRKRLSFIRLF*I EIISTIKVMLTIPLSAE*RKI*FNLLQFNYNTT FTTFSCVLNKYFLIG*RTFKQRNAFFT*NVYLIC CLFTKPLLC**KCPLCDVFDFTVC*ILFSWSCF LVTAPLLLPILLFYINACFVVIPQDVTLGMLG* GYEKILPKPGIQCMFVLYCLIRKVGPALSSTDAL VDA*SGTFFISFRSRDRSE*KGRRALLCSIHRL GDDLTGYTPLTSAYRLMHVNEYFCSCKA*QLQL HIYF*RIKQDHIYLLLSTIERKTFSCISTDFSQG KG*Y*SLLLSHPFV*VLKRDGGRSSFL*GDQYC NVCE*MITINITKSPT*ES*RPLHAPQ*CGFFH GFFFLLFS*SHPKHALFFLES*VFINCRCY*KKI KFSLKSKPCVLCL*FKSQK*F*FKQKDTKYTEV KFELATESFVFMSEFAKSGVDKALYGRLNNCK* MISKLNLARTSILKISVPRQCTARNKWPLQLPRL THCAIVAGGTVNLSSSL*YCPLCIPIFYSFFRR LLSANYSASKCK*MI*K*VGNMAESGASQPHNV GI*HRMRVRV*V*KIKSLVNNFVFIYSRCMYLRK EIWYFCFPIKGIKVMVFLSVL*AGLCYSSSSME MCFKICLPFE*SWLYVVEIFETYLVFTCAL*MN FVLFF*NLHITCRYYCNLYFA*A*SKVICVDE*L KNI*I

Frame 1

TGAPAWPSRRLRDLPAGGMWRLRRAAVACEVCQ SLVKHSSGIKGSLPLQKLHLVSRSIYHSHHPTL KLQRPQLRTSFQQFSSLTNLPLRKLKFSPIKYGY QPRRNFWPARLATRLLKLRYLILGSAVGGGYTA KKTFDQWKDMIPDLSEYKWIVPDIVWEIDEYID FEKIRKALPSSEDLVKLAPDFDKIVESLSLLKDF FTSGHKLVSEVIGASDLLLLLGSPEETAFRATD RGSESDKHFRKVSDKEKIDQLQEELLHTQLKYQ RILERLEKENKELRKLVLQKDDKGIHHRKLKKSL IDMYSEVLDVLSDYDASYNTQDHLPRVVVVGDQ SAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVT LSEGPHHVALFKDSSREFDLTKEEDLAALRHEIE LRMRKNVKEGCTVSPETISLNVKGPGLQRMVLV DLPGVINTVTSGMAPDTKETIFSISKAYMQNPN AIILCIQDGSVDAERSIVTDLVSQMDPHGRRTIF VLTKVDLAEKNVASPSRIQQIIEGKLFPMKALG YFAVVTGKGNSSESIEAIREYEEEFFQNSKLLK TSMLKAHQVTTRNLSLAVSDCFWKMVRESVEQQA DSFKATRFNLETEWKNNYPRLRELDRNELFEKA KNEILDEVISLSQVTPKHWEEILQQSLWERVST HVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQ LPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIF DKLKEAVKEESIKRHKWNDFAEDSLRVIQHNAL EDRSISDKQQWDAAIYFMEEALQARLKDTENAIE NMVGPDWKKRWLYWKNRTQEQCVHNETKNELEK MLKCNEEHPAYLASDEITTVRKNLESRGVEVDP SLIKDTWHQVYRRHFLKTALNHCNLCRRGFYYYQ RHFVDSELECNDVVLFWRIQRMLAITANTLRQQ LTNTEVRRLEKNVKEVLEDFAEDGEKKIKLLTG KRVQLAEDLKKVREIQEKLDAFIEALHQEK*IKI VLIISSAYI*RTKTSTSFVQPLFLLLFHLSKHT IKSWDKNNRCMLRAL*PSAASRMEEQW*WINIL FCCTKVTNRNNIIGMAIRFSP*R*ETCSLFVVLF VVALV*WIN*GCSMFSFFSRNTMLGVLK*NLYS NCLKLSIVYKITVAC*IIVCVCSILFPETI*P* *FSLYSPHERKMGNRRTLKTG*FGL*RSVKEIST LHSQRRNLPWKPSQYQLTYLIQLLFSHSLYPFE IDLF*MLYTYVRLSVNSGFSPVDRATGL*HRMR KIKDNQLTNFI*NIIKHFN*VSEKGFLS*DYFK* KLFQQLK*C*PSPSQLNKEKFSSIYCNLITILP SQHFHVF*INIF*LAKGHSSKEMLSLLKMSISF AAFSLSLYFVNKSVHCVMFLILQFAKSYFLGVAF W*QPHCYSPFYCFTSMHASL*SLKM*HLVCSVE DMKKYFRNQEFNVCLFYTV**EK*VQP*AAQMR W*MHSQELFLFLLGLGTGVNRKGGELYYVLYTD* EMTLLGTPL*PVLTG*CMLMNIFAVVKHNNYNY TSISKE*NRTIFIYFCQL*KERPSAVFPQISPK EKANISHYCYHIPLYKF*KEMEGDLHFFEEISIV TYVNR**QLILLKVPHESPDALSMPHSNVASFM GFFFFFLADLILSMLYFSLKARYLSTADVIERK *NSVSRVNPVSCVCSSKVRNDSNLNKKILNIQKL NSN*PQNHLFLCQNLQRVEWTKLCMED*TTVNR *YPNLIWLGLQF*KSVYLGSAQHEISGPCSFPV *PTVL*LRVEQSTFLVVYDIALFVFPFSTVFSAD FFLQIIQPPNANE*YKNK*GTWQRVVLPSLTMW EFDIG*ESEYRFKR*NL*LIILYLFILDVCI*G KKSGIFAFQ*RGSK*WFFSQFSKLVYVIALAVWK CALKYAYLLNDHGYMLLRYLKLTLFSLVHCE*T LYYFFKTFTLRVDIIATYILPELDQRSFV*MSN *KIFKS

Frame 2

RGLPRGRLGACVTSPPAGCGDYVGPLWPVRSAS L**NTALE*KEVYHYKNYIWFHEAFIIHIILP* SFNDPN*GHPFSSSLL*QTFLYVN*NSLQLNMA TSLAGIFGQQD*LRDS*NFAISY*DRLLGVATQP KRLLISGKI*YRTLVNINGLCLTLCGKLMSISI LRKLEKPFLVQKTL*S*HQTLTRLLKALAY*RT FLPQVTNWLVKS*ELLTYFSC*VLRKKRRLEQQI VDLKVTSILERCQTKRKLTNFRKNFCTLS*SIR ESWND*KRRTKN*EN*YCRKMTKAFIIESLRNL *LTCILKFLMFSLIMMPVIIRKIICHGLLWLEIR VLERLVCWK*LPKLEYSQEDLGR**HVLQLR*L *VKVLTMWPYLKIVLGSLILPKKKILQH*DMK* NFE*GKM*KKAVPLALRPYP*M*KALDYRGWCLL TYQV*LIL*HQAWLLTQRKLFSVSAKLTCRILM PSYCVFKMDLWMLNAVLLQTWSVKWTLMEGEPY SF*PK*TWQRKM*PVQAGFSR*LKESSSQ*KL*V ILLL*QEKGTALKALKL*ENMKKSFFRIQSS*R QAC*RHTK*LQEI*ALQYQTAFGKWYESLLNNR LIVSKQHVLTLKLNGRITILACGNLTGMNYLKKL KMKSLMKLSV*ARLHQNIGRKSFNNLCGKEYQL M*LKTSTFQLRRP*IQELLTPQWISSLNSGLIN NFLIKQ*RLLGRPYKKNFPAL*QNRKGKSMMTYL INLKRLLRKKVLNDTSGMTLRRTA*GLFNTMLW KTDPYLINSNGMQLFILWKRLCRLVSRILKMQL KTWWVQTGKRGGYTGRIGPKNSVFTMKPRMNWRR C*NVMRSTQLILQVMK*PQSGRTLNPEE*K*IQ A*LRILGIKFIEDIF*KQL*TIVTFVEEVFITT KGIL*ILSWNAMMWSCFGVYSACLLSPQIL*GNN LQILKLGD*RKMLKRYWKILLKMVRRRLNCLLV NAFNWRKTSRKLEKFKKNLMLSLKLFIRRNKLK SYS*SALHTSEEQKHQRLLSSLFFFCCSTFLNIQ *SHGIKIIDVCYGRFNHQLPLEWKNSGNGLTSY FVVLK*QIGII*LVWPLGSVLEDKKLVLCLLSY LWWHSFNGLTEVAQCSVSFPEIQC*VF*NKTYIA IV*SYQLYIKSQ*PAKSLYVSVVFYSQKLFDHD NSVYIHHMKEKWVTEEPLKQVNLDCNVQ*KKFQ PFIASEEICLGSQVSTSLPI*FSCCFLTLYIHLK LIYFRCCILTLGFLLIVVFLLLTEPPDYDTG*G RLRIIN*LISFRILSNISTRYQKKAFFHKTILN RNYFNN*SNVDHPPLS*IKKNLVQFIAI*LQYYL HNIFMCFK*IFFNWLKDIQAKKCFLYLKCLSHL LPFH*AFTLLIKVSIV*CF*FYSLLNLIFLELL FGNSPIATPHFIVLHQCMLRCDPSRCNTWYARLR I*KNTSETRNSMYVCFILFDKKSRSSLKQHRCA GRCIVRNFFYFF*V*GQE*IEREESSIMFYTQI RR*PYWVHPSNQCLQVNAC**IFLQL*SITITTT HLFLKNKTGPYLFTSVNYRKKDLQLYFHRFLPR KRLILVTTVITSLCISFKKRWREIFISLRRSVL *RM*IDDNN*YY*KSHMRVLTPSPCPTVMWLLSW VFFSSF*LISS*ACFIFP*KLGIYQLQMLLKEN KIQSQE*TLCLVSVVQKSEMILI*TKRY*IYRS *IRTSHRIICFYVRICKEWSGQSSVWKTEQL*ID DIQT*FG*DFNFKNQCT*AVHSTK*VALAASPF NPLCYSCGWNSQPF**FMILPSLYSHFLQFFPQ TSFCKLFSLQMQMNDIKISREHGREWCFPASQCG NLT*DESQSIGLKDKIFS**FCIYLF*MYVSEE RNLVFLLSNKGDQSNGFSLSSLSWSML*L*QYG NVL*NMLTF*MIMAICC*DI*NLPCFHLCTVNEL CIIFLKPSHYV*ILLQLIFCLSLIKGHLCR*VI KKYLNH