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Sequences

Sequence NM_001006620

TCCGGAACGCCGGGGTGTGGTTCCGGGTCGTGTGCGGCTCGGGGTAATAGGGCTGCTGCTCGGCCGGCCGGCGGCGGCGAGCAGCAGGGGCATGAGGGCT AACCCGGGAAGCGGCAGCTGAGCGGGCCGGGAGGAGCGCCGGTCCCCGTGGATCCCGAGAGTGCAGAGCTCGGGGCAGGGGCCGGGAGGCGTGGGGGAGC CGGGCCCTCCCCTCAGGAACGTGTCCCGGGGCCGACCCGGCCCGTAGTGTGGAAGCAGCTTCAGCTCAAAGATTAGAACGACTCCGAAAAGAGAGACAAA ACCAGATCAAATGCAAAAATATTCAGTGGAAAGAAAGAAATTCTAAGCAATCAGCCCAGGAGTTAAAGTCACTGTTTGAAAAAAAATCTCTCAAAGAGAA GCCTCCAATTTCTGGGAAGCAGTCGATATTATCTGTACGCCTAGAACAGTGCCCTCTGCAGCTGAATAACCCTTTTAACGAGTATTCCAAATTTGATGGC AAGGGTCATGTAGGTACAACAGCAACCAAGAAGATCGATGTCTACCTCCCTCTGCACTCGAGCCAGGACAGACTGCTGCCAATGACCGTGGTGACAATGG CCAGCGCCAGGGTGCAGGACCTGATCGGGCTCATCTGCTGGCAGTATACAAGCGAAGGACGGGAGCCGAAGCTCAATGACAATGTCAGTGCCTACTGCCT GCATATTGCTGAGGATGATGGGGAGGTGGACACCGATTTCCCCCCGCTGGATTCCAATGAGCCCATTCATAAGTTTGGCTTCAGTACTTTGGCCCTGGTT GAAAAGTACTCATCTCCTGGTCTGACATCCAAAGAGTCACTCTTTGTTCGAATAAATGCTGCTCATGGATTCTCCCTTATTCAGGTGGACAACACAAAGG TTACCATGAAGGAAATCTTACTGAAGGCAGTGAAGCGAAGAAAAGGATCCCAGAAAGTTTCAGGCCCTCAGTACCGCCTGGAGAAGCAGAGCGAGCCCAA TGTCGCCGTTGACCTGGACAGCACTTTGGAGAGCCAGAGCGCATGGGAGTTCTGCCTGGTCCGCGAGAACAGTTCAAGGGCAGACGGGGTTTTTGAGGAG GATTCGCAAATTGACATAGCCACAGTACAGGATATGCTTAGCAGCCACCATTACAAGTCATTCAAAGTCAGCATGATCCACAGACTGCGATTCACAACCG ACGTACAGCTAGGTATCTCTGGAGACAAAGTAGAGATAGACCCTGTTACGAATCAGAAAGCCAGCACTAAGTTTTGGATTAAGCAGAAACCCATCTCAAT CGATTCCGACCTGCTCTGTGCCTGTGACCTTGCTGAAGAGAAAAGCCCCAGTCACGCAATATTTAAACTCACGTATCTAAGCAATCACGACTATAAACAC CTCTACTTTGAATCGGACGCTGCTACCGTCAATGAAATTGTGCTCAAGGTTAACTACATCCTGGAATCGCGAGCTAGCACTGCCCGGGCTGACTACTTTG CTCAAAAACAAAGAAAACTGAACAGACGTACGAGCTTCAGCTTCCAGAAGGAGAAGAAATCCGGGCAGCAGTGACACTGGCCTCCAGCCTCAATCTGTTC CGTAGCTCAGAGCCTGCCTGCCAGGGCCAAGTGCCCTAGAGTCCACCCGGTGTCCTGAAGTCCTCGGGGGGAGGCCAGCCCCTGGCTCACTGGCACAGGG CAGGTGGGCTCTCGGGGAAGGTGTCGGGGGCCCCCTAGGAGGGAGCGCTGGGGACATTGCCATGGGACGGAAGTCTGCTTGGCAGTGGCTTTGATAAGCG ATGCTTGGGGGTCAGACCACCCCCTAGAGGAGCCACGTGCCGCCCAGCCACCTTCAATGCCTGCCACCCTGCCCGAGGATGTACAGAGCCGTGCCCACAC ATTTCCTTGCAACTTGATCAAATTTCTTAAAGCAAACAACAAAAATGTACATTTCTGTTTTTCCTTTTAATAAACAGGTGTACTCTTTATCATGGTTGGT ATGATGGACCATTCTTTGGGGCGGAGGATTGATTATGTTACTCTCTTTAAAATCTGTTCCCATATTGAACAGGCAGATTGGAAAAGCTATGGTTCGATTT CTCAGAAGAAATGTTTAGGTCTTAGTCAATAGTTTTAACTATGCCATTTGTTTAAATGAGTGCATTTGCTTCGAGGGTAGTGTCTTACTAAAAGTTAGGA ACAGAGACCTAGTGGTGTGTCCAAGGCCGTGTCACTTTCCCCTTCAGCACACCCCAGCTTCTGACCTCAGAGCCCAGGAGCTGCGTGGACAGTGTGGGGT GCCAGGAGGAGGGGCGGTGGCTGGTCCTCAGGCACGCTGCACTCCCAGCCAGACATGGTCTTTCCGTTTCTTAAGTAGCAAGTGTAGGTTTCAGCTGGCA GTTCCACCTGCATGTTCTCTGCTTCGCTGCCTTGGAAGGGGCCACATTCCCCATTCCTCTTCTCCTTACAGCGCCTGCCTCCTTTTTCAAGCAGGCGGAA AGCTGCTGTTTCTCACGTTTCAGGGAGAGGGGTGAGCGGAGGGAGACCTGTGTCCGTGCCGTCCGGCTCCCTGGGTGGGAACAGGCAAGGGATCAGATGC CCCTGACACCACGCCTCTGGCCACACCAGATGCCTCTGCAGTCCTCGACAGCCTCTTCAGTGTCCCTCCTGCGGTGATGTCCTTACTGTCCCCAGCCAGG GCCGGGGACCGGTGTTTCACTGAGGACCTGCATTAGAAACATTTTTTAAATTGTTGTACAGGAAGAGATGTGTCTAAAACAGCATCTTAAAGCTGAGTGT ATTTCTTTGCACAAGGGGTCATGCTGATGAATTCTTCTTTCATTCTGATCTTTGTTCAGCCAACAGGAGCGTCCTTTTCTAATGTCTTCCATTCCTACCC CCCACCCAAAAACAAAAGAAATATTTGTAGCTTGCTATCTGTATTTGAATTTTTAGCAATTTTATATTTAGATACTTTGAAAAATGTAAATGACTAATTT GGTCATTAAATCTTGTGACATATTCGATATTAAAATGATATTAAAATAAAAGTCATATAAATACACA

refseq_MAPKAP1.F5 (forward primer)
refseq_MAPKAP1.R5 (reverse primer)
Single exon skipping

Frame 0

SGTPGCGSGSCAARGNRAAARPAGGGEQQGHEG *PGKRQLSGPGGAPVPVDPESAELGAGAGRRGGA GPSPQERVPGPTRPVVWKQLQLKD*NDSEKRDK TRSNAKIFSGKKEILSNQPRS*SHCLKKNLSKR SLQFLGSSRYYLYA*NSALCS*ITLLTSIPNLMA RVM*VQQQPRRSMSTSLCTRARTDCCQ*PW*QW PAPGCRT*SGSSAGSIQAKDGSRSSMTMSVPTA CILLRMMGRWTPISPRWIPMSPFISLASVLWPWL KSTHLLV*HPKSHSLFE*MLLMDSPLFRWTTQR LP*RKSY*RQ*SEEKDPRKFQALSTAWRSRASP MSPLTWTALWRARAHGSSAWSARTVQGQTGFLRR IRKLT*PQYRICLAATITSHSKSA*STDCDSQP TYS*VSLETK*R*TLLRIRKPALSFGLSRNPSQ SIPTCSVPVTLLKRKAPVTQYLNSRI*AITTINT STLNRTLLPSMKLCSRLTTSWNRELALPGLTTL LKNKEN*TDVRASASRRRRNPGSSDTGLQPQSV P*LRACLPGPSALESTRCPEVLGGRPAPGSLAQG RWALGEGVGGPLGGSAGDIAMGRKSAWQWL**A MLGGQTTP*RSHVPPSHLQCLPPCPRMYRAVPT HFLAT*SNFLKQTTKMYISVFPFNKQVYSLSWLV *WTILWGGGLIMLLSLKSVPILNRQIGKAMVRF LRRNV*VLVNSFNYAICLNECICFEGSVLLKVR NRDLVVCPRPCHFPLQHTPASDLRAQELRGQCGV PGGGAVAGPQARCTPSQTWSFRFLSSKCRFQLA VPPACSLLRCLGRGHIPHSSSPYSACLLFQAGG KLLFLTFQGEG*AEGDLCPCRPAPWVGTGKGSDA PDTTPLATPDASAVLDSLFSVPPAVMSLLSPAR AGDRCFTEDLH*KHFLNCCTGRDVSKTAS*S*V YFFAQGVMLMNSSFILIFVQPTGASFSNVFHSYP PPKNKRNICSLLSVFEFLAILYLDTLKNVND*F GH*IL*HIRY*NDIKIKVI*IH

Frame 1

PERRGVVPGRVRLGVIGLLLGRPAAASSRGMRA NPGSGS*AGREERRSPWIPRVQSSGQGPGGVGE PGPPLRNVSRGRPGP*CGSSFSSKIRTTPKRETK PDQMQKYSVERKKF*AISPGVKVTV*KKISQRE ASNFWEAVDIICTPRTVPSAAE*PF*RVFQI*W QGSCRYNSNQEDRCLPPSALEPGQTAANDRGDNG QRQGAGPDRAHLLAVYKRRTGAEAQ*QCQCLLP AYC*G*WGGGHRFPPAGFQ*AHS*VWLQYFGPG *KVLISWSDIQRVTLCSNKCCSWILPYSGGQHKG YHEGNLTEGSEAKKRIPESFRPSVPPGEAERAQ CRR*PGQHFGEPERMGVLPGPREQFKGRRGF*G GFAN*HSHSTGYA*QPPLQVIQSQHDPQTAIHNR RTARYLWRQSRDRPCYESESQH*VLD*AETHLN RFRPALCL*PC*REKPQSRNI*THVSKQSRL*T PLL*IGRCYRQ*NCAQG*LHPGIAS*HCPG*LLC SKTKKTEQTYELQLPEGEEIRAAVTLASSLNLF RSSEPACQGQVP*SPPGVLKSSGGGQPLAHWHR AGGLSGKVSGAP*EGALGTLPWDGSLLGSGFDKR CLGVRPPPRGATCRPATFNACHPARGCTEPCPH ISLQLDQIS*SKQQKCTFLFFLLINRCTLYHGW YDGPFFGAED*LCYSL*NLFPY*TGRLEKLWFDF SEEMFRS*SIVLTMPFV*MSAFASRVVSY*KLG TET*WCVQGRVTFPFSTPQLLTSEPRSCVDSVG CQEEGRWLVLRHAALPARHGLSVS*VASVGFSWQ FHLHVLCFAALEGATFPIPLLLTAPASFFKQAE SCCFSRFRERGERRETCVRAVRLPGWEQARDQM PLTPRLWPHQMPLQSSTASSVSLLR*CPYCPQPG PGTGVSLRTCIRNIF*IVVQEEMCLKQHLKAEC ISLHKGSC**ILLSF*SLFSQQERPFLMSSIPT PHPKTKEIFVACYLYLNF*QFYI*IL*KM*MTNL VIKSCDIFDIKMILK*KSYKYT

Frame 2

RNAGVWFRVVCGSG**GCCSAGRRRRAAGA*GL TREAAAERAGRSAGPRGSRECRARGRGREAWGS RALPSGTCPGADPARSVEAASAQRLERLRKERQ NQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEK PPISGKQSILSVRLEQCPLQLNNPFNEYSKFDG KGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTM ASARVQDLIGLICWQYTSEGREPKLNDNVSAYCL HIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALV EKYSSPGLTSKESLFVRINAAHGFSLIQVDNTK VTMKEILLKAVKRRKGSQKVSGPQYRLEKQSEPN VAVDLDSTLESQSAWEFCLVRENSSRADGVFEE DSQIDIATVQDMLSSHHYKSFKVSMIHRLRFTT DVQLGISGDKVEIDPVTNQKASTKFWIKQKPISI DSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKH LYFESDAATVNEIVLKVNYILESRASTARADYF AQKQRKLNRRTSFSFQKEKKSGQQ*HWPPASICS VAQSLPARAKCPRVHPVS*SPRGEASPWLTGTG QVGSRGRCRGPPRRERWGHCHGTEVCLAVALIS DAWGSDHPLEEPRAAQPPSMPATLPEDVQSRAHT FPCNLIKFLKANNKNVHFCFSF**TGVLFIMVG MMDHSLGRRIDYVTLFKICSHIEQADWKSYGSI SQKKCLGLSQ*F*LCHLFK*VHLLRG*CLTKS*E QRPSGVSKAVSLSPSAHPSF*PQSPGAAWTVWG ARRRGGGWSSGTLHSQPDMVFPFLK*QV*VSAG SSTCMFSASLPWKGPHSPFLFSLQRLPPFSSRRK AAVSHVSGRGVSGGRPVSVPSGSLGGNRQGIRC P*HHASGHTRCLCSPRQPLQCPSCGDVLTVPSQ GRGPVFH*GPALETFFKLLYRKRCV*NSILKLSV FLCTRGHADEFFFHSDLCSANRSVLF*CLPFLP PTQKQKKYL*LAICI*IFSNFIFRYFEKCK*LI WSLNLVTYSILK*Y*NKSHINT

Sequence NM_001006618

refseq_MAPKAP1.F5 (forward primer)
refseq_MAPKAP1.R5 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence NM_001006619

TCCGGAACGCCGGGGTGTGGTTCCGGGTCGTGTGCGGCTCGGGGTAATAGGGCTGCTGCTCGGCCGGCCGGCGGCGGCGAGCAGCAGGGGCATGAGGGCT AACCCGGGAAGCGGCAGCTGAGCGGGCCGGGAGGAGCGCCGGTCCCCGTGGATCCCGAGAGTGCAGAGCTCGGGGCAGGGGCCGGGAGGCGTGGGGGAGC CGGGCCCTCCCCTCAGGAACGTGTCCCGGGGCCGACCCGGCCCGTAGTGTGGAAGCAGCTTCAGGTAGGTGAGCTCGTGAAACAATATGAAGAGGAGAAA ATAGCCTTTTAAGGAAATTGGCCCACAGAAAGGATGGCCTTCTTGGACAATCCAACTATCATTCTAGCTCATATTCGACAGTCACATGTGACCAGTGATG ACACGGGAATGTGTGAGATGGTTCTCATTGATCATGATGTTGACCTAGAGAAGATTCATCCTCCTTCAATGCCTGGAGACAGTGGGTCAGAAATTCAGGG AAGCAATGGTGAGACTCAGGGCTATGTATATGCCCAGTCAGTCGATATTACCTCAAGTTGGGACTTTGGTATTAGAAGACGCTCAAACACAGCTCAAAGA TTAGAACGACTCCGAAAAGAGAGACAAAACCAGATCAAATGCAAAAATATTCAGTGGAAAGAAAGAAATTCTAAGCAATCAGCCCAGGAGTTAAAGTCAC TGTTTGAAAAAAAATCTCTCAAAGAGAAGCCTCCAATTTCTGGGAAGCAGTCGATATTATCTGTACGCCTAGAACAGTGCCCTCTGCAGCTGAATAACCC TTTTAACGAGTATTCCAAATTTGATGGCAAGGGTCATGTAGGTACAACAGCAACCAAGAAGATCGATGTCTACCTCCCTCTGCACTCGAGCCAGGACAGA CTGCTGCCAATGACCGTGGTGACAATGGCCAGCGCCAGGGTGCAGGACCTGATCGGGCTCATCTGCTGGCAGTATACAAGCGAAGGACGGGAGCCGAAGC TCAATGACAATGTCAGTGCCTACTGCCTGCATATTGCTGAGGATGATGGGGAGGTGGACACCGATTTCCCCCCGCTGGATTCCAATGAGCCCATTCATAA GTTTGGCTTCAGTACTTTGGCCCTGGTTGAAAAGTACTCATCTCCTGGTCTGACATCCAAAGAGTCACTCTTTGTTCGAATAAATGCTGCTCATGGATTC TCCCTTATTCAGGTGGACAACACAAAGGTTACCATGAAGGAAATCTTACTGAAGGCAGTGAAGCGAAGAAAAGGATCCCAGAAAGTTTCAGGCCCTCAGT ACCGCCTGGAGAAGCAGAGCGAGCCCAATGTCGCCGTTGACCTGGACAGCACTTTGGAGAGCCAGAGCGCATGGGAGTTCTGCCTGGTCCGCGAGAACAG TATCTCTGGAGACAAAGTAGAGATAGACCCTGTTACGAATCAGAAAGCCAGCACTAAGTTTTGGATTAAGCAGAAACCCATCTCAATCGATTCCGACCTG CTCTGTGCCTGTGACCTTGCTGAAGAGAAAAGCCCCAGTCACGCAATATTTAAACTCACGTATCTAAGCAATCACGACTATAAACACCTCTACTTTGAAT CGGACGCTGCTACCGTCAATGAAATTGTGCTCAAGGTTAACTACATCCTGGAATCGCGAGCTAGCACTGCCCGGGCTGACTACTTTGCTCAAAAACAAAG AAAACTGAACAGACGTACGAGCTTCAGCTTCCAGAAGGAGAAGAAATCCGGGCAGCAGTGACACTGGCCTCCAGCCTCAATCTGTTCCGTAGCTCAGAGC CTGCCTGCCAGGGCCAAGTGCCCTAGAGTCCACCCGGTGTCCTGAAGTCCTCGGGGGGAGGCCAGCCCCTGGCTCACTGGCACAGGGCAGGTGGGCTCTC GGGGAAGGTGTCGGGGGCCCCCTAGGAGGGAGCGCTGGGGACATTGCCATGGGACGGAAGTCTGCTTGGCAGTGGCTTTGATAAGCGATGCTTGGGGGTC AGACCACCCCCTAGAGGAGCCACGTGCCGCCCAGCCACCTTCAATGCCTGCCACCCTGCCCGAGGATGTACAGAGCCGTGCCCACACATTTCCTTGCAAC TTGATCAAATTTCTTAAAGCAAACAACAAAAATGTACATTTCTGTTTTTCCTTTTAATAAACAGGTGTACTCTTTATCATGGTTGGTATGATGGACCATT CTTTGGGGCGGAGGATTGATTATGTTACTCTCTTTAAAATCTGTTCCCATATTGAACAGGCAGATTGGAAAAGCTATGGTTCGATTTCTCAGAAGAAATG TTTAGGTCTTAGTCAATAGTTTTAACTATGCCATTTGTTTAAATGAGTGCATTTGCTTCGAGGGTAGTGTCTTACTAAAAGTTAGGAACAGAGACCTAGT GGTGTGTCCAAGGCCGTGTCACTTTCCCCTTCAGCACACCCCAGCTTCTGACCTCAGAGCCCAGGAGCTGCGTGGACAGTGTGGGGTGCCAGGAGGAGGG GCGGTGGCTGGTCCTCAGGCACGCTGCACTCCCAGCCAGACATGGTCTTTCCGTTTCTTAAGTAGCAAGTGTAGGTTTCAGCTGGCAGTTCCACCTGCAT GTTCTCTGCTTCGCTGCCTTGGAAGGGGCCACATTCCCCATTCCTCTTCTCCTTACAGCGCCTGCCTCCTTTTTCAAGCAGGCGGAAAGCTGCTGTTTCT CACGTTTCAGGGAGAGGGGTGAGCGGAGGGAGACCTGTGTCCGTGCCGTCCGGCTCCCTGGGTGGGAACAGGCAAGGGATCAGATGCCCCTGACACCACG CCTCTGGCCACACCAGATGCCTCTGCAGTCCTCGACAGCCTCTTCAGTGTCCCTCCTGCGGTGATGTCCTTACTGTCCCCAGCCAGGGCCGGGGACCGGT GTTTCACTGAGGACCTGCATTAGAAACATTTTTTAAATTGTTGTACAGGAAGAGATGTGTCTAAAACAGCATCTTAAAGCTGAGTGTATTTCTTTGCACA AGGGGTCATGCTGATGAATTCTTCTTTCATTCTGATCTTTGTTCAGCCAACAGGAGCGTCCTTTTCTAATGTCTTCCATTCCTACCCCCCACCCAAAAAC AAAAGAAATATTTGTAGCTTGCTATCTGTATTTGAATTTTTAGCAATTTTATATTTAGATACTTTGAAAAATGTAAATGACTAATTTGGTCATTAAATCT TGTGACATATTCGATATTAAAATGATATTAAAATAAAAGTCATATAAATACACA

refseq_MAPKAP1.F5 (forward primer)
refseq_MAPKAP1.R5 (reverse primer)
Single exon skipping

Frame 0

SGTPGCGSGSCAARGNRAAARPAGGGEQQGHEG *PGKRQLSGPGGAPVPVDPESAELGAGAGRRGGA GPSPQERVPGPTRPVVWKQLQVGELVKQYEEEK IAF*GNWPTERMAFLDNPTIILAHIRQSHVTSD DTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQG SNGETQGYVYAQSVDITSSWDFGIRRRSNTAQR LERLRKERQNQIKCKNIQWKERNSKQSAQELKS LFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNP FNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDR LLPMTVVTMASARVQDLIGLICWQYTSEGREPK LNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHK FGFSTLALVEKYSSPGLTSKESLFVRINAAHGF SLIQVDNTKVTMKEILLKAVKRRKGSQKVSGPQ YRLEKQSEPNVAVDLDSTLESQSAWEFCLVRENS ISGDKVEIDPVTNQKASTKFWIKQKPISIDSDL LCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFE SDAATVNEIVLKVNYILESRASTARADYFAQKQR KLNRRTSFSFQKEKKSGQQ*HWPPASICSVAQS LPARAKCPRVHPVS*SPRGEASPWLTGTGQVGS RGRCRGPPRRERWGHCHGTEVCLAVALISDAWGS DHPLEEPRAAQPPSMPATLPEDVQSRAHTFPCN LIKFLKANNKNVHFCFSF**TGVLFIMVGMMDH SLGRRIDYVTLFKICSHIEQADWKSYGSISQKKC LGLSQ*F*LCHLFK*VHLLRG*CLTKS*EQRPS GVSKAVSLSPSAHPSF*PQSPGAAWTVWGARRR GGGWSSGTLHSQPDMVFPFLK*QV*VSAGSSTCM FSASLPWKGPHSPFLFSLQRLPPFSSRRKAAVS HVSGRGVSGGRPVSVPSGSLGGNRQGIRCP*HH ASGHTRCLCSPRQPLQCPSCGDVLTVPSQGRGPV FH*GPALETFFKLLYRKRCV*NSILKLSVFLCT RGHADEFFFHSDLCSANRSVLF*CLPFLPPTQK QKKYL*LAICI*IFSNFIFRYFEKCK*LIWSLNL VTYSILK*Y*NKSHINT

Frame 1

PERRGVVPGRVRLGVIGLLLGRPAAASSRGMRA NPGSGS*AGREERRSPWIPRVQSSGQGPGGVGE PGPPLRNVSRGRPGP*CGSSFR*VSS*NNMKRRK *PFKEIGPQKGWPSWTIQLSF*LIFDSHM*PVM TRECVRWFSLIMMLT*RRFILLQCLETVGQKFR EAMVRLRAMYMPSQSILPQVGTLVLEDAQTQLKD *NDSEKRDKTRSNAKIFSGKKEILSNQPRS*SH CLKKNLSKRSLQFLGSSRYYLYA*NSALCS*IT LLTSIPNLMARVM*VQQQPRRSMSTSLCTRARTD CCQ*PW*QWPAPGCRT*SGSSAGSIQAKDGSRS SMTMSVPTACILLRMMGRWTPISPRWIPMSPFI SLASVLWPWLKSTHLLV*HPKSHSLFE*MLLMDS PLFRWTTQRLP*RKSY*RQ*SEEKDPRKFQALS TAWRSRASPMSPLTWTALWRARAHGSSAWSART VSLETK*R*TLLRIRKPALSFGLSRNPSQSIPTC SVPVTLLKRKAPVTQYLNSRI*AITTINTSTLN RTLLPSMKLCSRLTTSWNRELALPGLTTLLKNK EN*TDVRASASRRRRNPGSSDTGLQPQSVP*LRA CLPGPSALESTRCPEVLGGRPAPGSLAQGRWAL GEGVGGPLGGSAGDIAMGRKSAWQWL**AMLGG QTTP*RSHVPPSHLQCLPPCPRMYRAVPTHFLAT *SNFLKQTTKMYISVFPFNKQVYSLSWLV*WTI LWGGGLIMLLSLKSVPILNRQIGKAMVRFLRRN V*VLVNSFNYAICLNECICFEGSVLLKVRNRDLV VCPRPCHFPLQHTPASDLRAQELRGQCGVPGGG AVAGPQARCTPSQTWSFRFLSSKCRFQLAVPPA CSLLRCLGRGHIPHSSSPYSACLLFQAGGKLLFL TFQGEG*AEGDLCPCRPAPWVGTGKGSDAPDTT PLATPDASAVLDSLFSVPPAVMSLLSPARAGDR CFTEDLH*KHFLNCCTGRDVSKTAS*S*VYFFAQ GVMLMNSSFILIFVQPTGASFSNVFHSYPPPKN KRNICSLLSVFEFLAILYLDTLKNVND*FGH*I L*HIRY*NDIKIKVI*IH

Frame 2

RNAGVWFRVVCGSG**GCCSAGRRRRAAGA*GL TREAAAERAGRSAGPRGSRECRARGRGREAWGS RALPSGTCPGADPARSVEAASGR*ARETI*RGE NSLLRKLAHRKDGLLGQSNYHSSSYSTVTCDQ** HGNV*DGSH*S*C*PREDSSSFNAWRQWVRNSG KQW*DSGLCICPVSRYYLKLGLWY*KTLKHSSK IRTTPKRETKPDQMQKYSVERKKF*AISPGVKVT V*KKISQREASNFWEAVDIICTPRTVPSAAE*P F*RVFQI*WQGSCRYNSNQEDRCLPPSALEPGQ TAANDRGDNGQRQGAGPDRAHLLAVYKRRTGAEA Q*QCQCLLPAYC*G*WGGGHRFPPAGFQ*AHS* VWLQYFGPG*KVLISWSDIQRVTLCSNKCCSWI LPYSGGQHKGYHEGNLTEGSEAKKRIPESFRPSV PPGEAERAQCRR*PGQHFGEPERMGVLPGPREQ YLWRQSRDRPCYESESQH*VLD*AETHLNRFRP ALCL*PC*REKPQSRNI*THVSKQSRL*TPLL*I GRCYRQ*NCAQG*LHPGIAS*HCPG*LLCSKTK KTEQTYELQLPEGEEIRAAVTLASSLNLFRSSE PACQGQVP*SPPGVLKSSGGGQPLAHWHRAGGLS GKVSGAP*EGALGTLPWDGSLLGSGFDKRCLGV RPPPRGATCRPATFNACHPARGCTEPCPHISLQ LDQIS*SKQQKCTFLFFLLINRCTLYHGWYDGPF FGAED*LCYSL*NLFPY*TGRLEKLWFDFSEEM FRS*SIVLTMPFV*MSAFASRVVSY*KLGTET* WCVQGRVTFPFSTPQLLTSEPRSCVDSVGCQEEG RWLVLRHAALPARHGLSVS*VASVGFSWQFHLH VLCFAALEGATFPIPLLLTAPASFFKQAESCCF SRFRERGERRETCVRAVRLPGWEQARDQMPLTPR LWPHQMPLQSSTASSVSLLR*CPYCPQPGPGTG VSLRTCIRNIF*IVVQEEMCLKQHLKAECISLH KGSC**ILLSF*SLFSQQERPFLMSSIPTPHPKT KEIFVACYLYLNF*QFYI*IL*KM*MTNLVIKS CDIFDIKMILK*KSYKYT

Sequence NM_024117

TCCGGAACGCCGGGGTGTGGTTCCGGGTCGTGTGCGGCTCGGGGTAATAGGGCTGCTGCTCGGCCGGCCGGCGGCGGCGAGCAGCAGGGGCATGAGGGCT AACCCGGGAAGCGGCAGCTGAGCGGGCCGGGAGGAGCGCCGGTCCCCGTGGATCCCGAGAGTGCAGAGCTCGGGGCAGGGGCCGGGAGGCGTGGGGGAGC CGGGCCCTCCCCTCAGGAACGTGTCCCGGGGCCGACCCGGCCCGTAGTGTGGAAGCAGCTTCAGGTAGGTGAGCTCGTGAAACAATATGAAGAGGAGAAA ATAGCCTTTTAAGGAAATTGGCCCACAGAAAGGATGGCCTTCTTGGACAATCCAACTATCATTCTAGCTCATATTCGACAGTCACATGTGACCAGTGATG ACACGGGAATGTGTGAGATGGTTCTCATTGATCATGATGTTGACCTAGAGAAGATTCATCCTCCTTCAATGCCTGGAGACAGTGGGTCAGAAATTCAGGG AAGCAATGGTGAGACTCAGGGCTATGTATATGCCCAGTCAGTCGATATTACCTCAAGTTGGGACTTTGGTATTAGAAGACGCTCAAACACAGCTCAAAGA TTAGAACGACTCCGAAAAGAGAGACAAAACCAGATCAAATGCAAAAATATTCAGTGGAAAGAAAGAAATTCTAAGCAATCAGCCCAGGAGTTAAAGTCAC TGTTTGAAAAAAAATCTCTCAAAGAGAAGCCTCCAATTTCTGGGAAGCAGTCGATATTATCTGTACGCCTAGAACAGTGCCCTCTGCAGCTGAATAACCC TTTTAACGAGTATTCCAAATTTGATGGCAAGGGTCATGTAGGTACAACAGCAACCAAGAAGATCGATGTCTACCTCCCTCTGCACTCGAGCCAGGACAGA CTGCTGCCAATGACCGTGGTGACAATGGCCAGCGCCAGGGTGCAGGACCTGATCGGGCTCATCTGCTGGCAGTATACAAGCGAAGGACGGGAGCCGAAGC TCAATGACAATGTCAGTGCCTACTGCCTGCATATTGCTGAGGATGATGGGGAGGTGGACACCGATTTCCCCCCGCTGGATTCCAATGAGCCCATTCATAA GTTTGGCTTCAGTACTTTGGCCCTGGTTGAAAAGTACTCATCTCCTGGTCTGACATCCAAAGAGTCACTCTTTGTTCGAATAAATGCTGCTCATGGATTC TCCCTTATTCAGGTGGACAACACAAAGGTTACCATGAAGGAAATCTTACTGAAGGCAGTGAAGCGAAGAAAAGGATCCCAGAAAGTTTCAGGTTCAAGGG CAGACGGGGTTTTTGAGGAGGATTCGCAAATTGACATAGCCACAGTACAGGATATGCTTAGCAGCCACCATTACAAGTCATTCAAAGTCAGCATGATCCA CAGACTGCGATTCACAACCGACGTACAGCTAGGTATCTCTGGAGACAAAGTAGAGATAGACCCTGTTACGAATCAGAAAGCCAGCACTAAGTTTTGGATT AAGCAGAAACCCATCTCAATCGATTCCGACCTGCTCTGTGCCTGTGACCTTGCTGAAGAGAAAAGCCCCAGTCACGCAATATTTAAACTCACGTATCTAA GCAATCACGACTATAAACACCTCTACTTTGAATCGGACGCTGCTACCGTCAATGAAATTGTGCTCAAGGTTAACTACATCCTGGAATCGCGAGCTAGCAC TGCCCGGGCTGACTACTTTGCTCAAAAACAAAGAAAACTGAACAGACGTACGAGCTTCAGCTTCCAGAAGGAGAAGAAATCCGGGCAGCAGTGACACTGG CCTCCAGCCTCAATCTGTTCCGTAGCTCAGAGCCTGCCTGCCAGGGCCAAGTGCCCTAGAGTCCACCCGGTGTCCTGAAGTCCTCGGGGGGAGGCCAGCC CCTGGCTCACTGGCACAGGGCAGGTGGGCTCTCGGGGAAGGTGTCGGGGGCCCCCTAGGAGGGAGCGCTGGGGACATTGCCATGGGACGGAAGTCTGCTT GGCAGTGGCTTTGATAAGCGATGCTTGGGGGTCAGACCACCCCCTAGAGGAGCCACGTGCCGCCCAGCCACCTTCAATGCCTGCCACCCTGCCCGAGGAT GTACAGAGCCGTGCCCACACATTTCCTTGCAACTTGATCAAATTTCTTAAAGCAAACAACAAAAATGTACATTTCTGTTTTTCCTTTTAATAAACAGGTG TACTCTTTATCATGGTTGGTATGATGGACCATTCTTTGGGGCGGAGGATTGATTATGTTACTCTCTTTAAAATCTGTTCCCATATTGAACAGGCAGATTG GAAAAGCTATGGTTCGATTTCTCAGAAGAAATGTTTAGGTCTTAGTCAATAGTTTTAACTATGCCATTTGTTTAAATGAGTGCATTTGCTTCGAGGGTAG TGTCTTACTAAAAGTTAGGAACAGAGACCTAGTGGTGTGTCCAAGGCCGTGTCACTTTCCCCTTCAGCACACCCCAGCTTCTGACCTCAGAGCCCAGGAG CTGCGTGGACAGTGTGGGGTGCCAGGAGGAGGGGCGGTGGCTGGTCCTCAGGCACGCTGCACTCCCAGCCAGACATGGTCTTTCCGTTTCTTAAGTAGCA AGTGTAGGTTTCAGCTGGCAGTTCCACCTGCATGTTCTCTGCTTCGCTGCCTTGGAAGGGGCCACATTCCCCATTCCTCTTCTCCTTACAGCGCCTGCCT CCTTTTTCAAGCAGGCGGAAAGCTGCTGTTTCTCACGTTTCAGGGAGAGGGGTGAGCGGAGGGAGACCTGTGTCCGTGCCGTCCGGCTCCCTGGGTGGGA ACAGGCAAGGGATCAGATGCCCCTGACACCACGCCTCTGGCCACACCAGATGCCTCTGCAGTCCTCGACAGCCTCTTCAGTGTCCCTCCTGCGGTGATGT CCTTACTGTCCCCAGCCAGGGCCGGGGACCGGTGTTTCACTGAGGACCTGCATTAGAAACATTTTTTAAATTGTTGTACAGGAAGAGATGTGTCTAAAAC AGCATCTTAAAGCTGAGTGTATTTCTTTGCACAAGGGGTCATGCTGATGAATTCTTCTTTCATTCTGATCTTTGTTCAGCCAACAGGAGCGTCCTTTTCT AATGTCTTCCATTCCTACCCCCCACCCAAAAACAAAAGAAATATTTGTAGCTTGCTATCTGTATTTGAATTTTTAGCAATTTTATATTTAGATACTTTGA AAAATGTAAATGACTAATTTGGTCATTAAATCTTGTGACATATTCGATATTAAAATGATATTAAAATAAAAGTCATATAAATACACA

refseq_MAPKAP1.F5 (forward primer)
refseq_MAPKAP1.R5 (reverse primer)
Single exon skipping

Frame 0

SGTPGCGSGSCAARGNRAAARPAGGGEQQGHEG *PGKRQLSGPGGAPVPVDPESAELGAGAGRRGGA GPSPQERVPGPTRPVVWKQLQVGELVKQYEEEK IAF*GNWPTERMAFLDNPTIILAHIRQSHVTSD DTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQG SNGETQGYVYAQSVDITSSWDFGIRRRSNTAQR LERLRKERQNQIKCKNIQWKERNSKQSAQELKS LFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNP FNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDR LLPMTVVTMASARVQDLIGLICWQYTSEGREPK LNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHK FGFSTLALVEKYSSPGLTSKESLFVRINAAHGF SLIQVDNTKVTMKEILLKAVKRRKGSQKVSGSR ADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIH RLRFTTDVQLGISGDKVEIDPVTNQKASTKFWI KQKPISIDSDLLCACDLAEEKSPSHAIFKLTYL SNHDYKHLYFESDAATVNEIVLKVNYILESRAST ARADYFAQKQRKLNRRTSFSFQKEKKSGQQ*HW PPASICSVAQSLPARAKCPRVHPVS*SPRGEAS PWLTGTGQVGSRGRCRGPPRRERWGHCHGTEVCL AVALISDAWGSDHPLEEPRAAQPPSMPATLPED VQSRAHTFPCNLIKFLKANNKNVHFCFSF**TG VLFIMVGMMDHSLGRRIDYVTLFKICSHIEQADW KSYGSISQKKCLGLSQ*F*LCHLFK*VHLLRG* CLTKS*EQRPSGVSKAVSLSPSAHPSF*PQSPG AAWTVWGARRRGGGWSSGTLHSQPDMVFPFLK*Q V*VSAGSSTCMFSASLPWKGPHSPFLFSLQRLP PFSSRRKAAVSHVSGRGVSGGRPVSVPSGSLGG NRQGIRCP*HHASGHTRCLCSPRQPLQCPSCGDV LTVPSQGRGPVFH*GPALETFFKLLYRKRCV*N SILKLSVFLCTRGHADEFFFHSDLCSANRSVLF *CLPFLPPTQKQKKYL*LAICI*IFSNFIFRYFE KCK*LIWSLNLVTYSILK*Y*NKSHINT

Frame 1

PERRGVVPGRVRLGVIGLLLGRPAAASSRGMRA NPGSGS*AGREERRSPWIPRVQSSGQGPGGVGE PGPPLRNVSRGRPGP*CGSSFR*VSS*NNMKRRK *PFKEIGPQKGWPSWTIQLSF*LIFDSHM*PVM TRECVRWFSLIMMLT*RRFILLQCLETVGQKFR EAMVRLRAMYMPSQSILPQVGTLVLEDAQTQLKD *NDSEKRDKTRSNAKIFSGKKEILSNQPRS*SH CLKKNLSKRSLQFLGSSRYYLYA*NSALCS*IT LLTSIPNLMARVM*VQQQPRRSMSTSLCTRARTD CCQ*PW*QWPAPGCRT*SGSSAGSIQAKDGSRS SMTMSVPTACILLRMMGRWTPISPRWIPMSPFI SLASVLWPWLKSTHLLV*HPKSHSLFE*MLLMDS PLFRWTTQRLP*RKSY*RQ*SEEKDPRKFQVQG QTGFLRRIRKLT*PQYRICLAATITSHSKSA*S TDCDSQPTYS*VSLETK*R*TLLRIRKPALSFGL SRNPSQSIPTCSVPVTLLKRKAPVTQYLNSRI* AITTINTSTLNRTLLPSMKLCSRLTTSWNRELA LPGLTTLLKNKEN*TDVRASASRRRRNPGSSDTG LQPQSVP*LRACLPGPSALESTRCPEVLGGRPA PGSLAQGRWALGEGVGGPLGGSAGDIAMGRKSA WQWL**AMLGGQTTP*RSHVPPSHLQCLPPCPRM YRAVPTHFLAT*SNFLKQTTKMYISVFPFNKQV YSLSWLV*WTILWGGGLIMLLSLKSVPILNRQI GKAMVRFLRRNV*VLVNSFNYAICLNECICFEGS VLLKVRNRDLVVCPRPCHFPLQHTPASDLRAQE LRGQCGVPGGGAVAGPQARCTPSQTWSFRFLSS KCRFQLAVPPACSLLRCLGRGHIPHSSSPYSACL LFQAGGKLLFLTFQGEG*AEGDLCPCRPAPWVG TGKGSDAPDTTPLATPDASAVLDSLFSVPPAVM SLLSPARAGDRCFTEDLH*KHFLNCCTGRDVSKT AS*S*VYFFAQGVMLMNSSFILIFVQPTGASFS NVFHSYPPPKNKRNICSLLSVFEFLAILYLDTL KNVND*FGH*IL*HIRY*NDIKIKVI*IH

Frame 2

RNAGVWFRVVCGSG**GCCSAGRRRRAAGA*GL TREAAAERAGRSAGPRGSRECRARGRGREAWGS RALPSGTCPGADPARSVEAASGR*ARETI*RGE NSLLRKLAHRKDGLLGQSNYHSSSYSTVTCDQ** HGNV*DGSH*S*C*PREDSSSFNAWRQWVRNSG KQW*DSGLCICPVSRYYLKLGLWY*KTLKHSSK IRTTPKRETKPDQMQKYSVERKKF*AISPGVKVT V*KKISQREASNFWEAVDIICTPRTVPSAAE*P F*RVFQI*WQGSCRYNSNQEDRCLPPSALEPGQ TAANDRGDNGQRQGAGPDRAHLLAVYKRRTGAEA Q*QCQCLLPAYC*G*WGGGHRFPPAGFQ*AHS* VWLQYFGPG*KVLISWSDIQRVTLCSNKCCSWI LPYSGGQHKGYHEGNLTEGSEAKKRIPESFRFKG RRGF*GGFAN*HSHSTGYA*QPPLQVIQSQHDP QTAIHNRRTARYLWRQSRDRPCYESESQH*VLD *AETHLNRFRPALCL*PC*REKPQSRNI*THVSK QSRL*TPLL*IGRCYRQ*NCAQG*LHPGIAS*H CPG*LLCSKTKKTEQTYELQLPEGEEIRAAVTL ASSLNLFRSSEPACQGQVP*SPPGVLKSSGGGQP LAHWHRAGGLSGKVSGAP*EGALGTLPWDGSLL GSGFDKRCLGVRPPPRGATCRPATFNACHPARG CTEPCPHISLQLDQIS*SKQQKCTFLFFLLINRC TLYHGWYDGPFFGAED*LCYSL*NLFPY*TGRL EKLWFDFSEEMFRS*SIVLTMPFV*MSAFASRV VSY*KLGTET*WCVQGRVTFPFSTPQLLTSEPRS CVDSVGCQEEGRWLVLRHAALPARHGLSVS*VA SVGFSWQFHLHVLCFAALEGATFPIPLLLTAPA SFFKQAESCCFSRFRERGERRETCVRAVRLPGWE QARDQMPLTPRLWPHQMPLQSSTASSVSLLR*C PYCPQPGPGTGVSLRTCIRNIF*IVVQEEMCLK QHLKAECISLHKGSC**ILLSF*SLFSQQERPFL MSSIPTPHPKTKEIFVACYLYLNF*QFYI*IL* KM*MTNLVIKSCDIFDIKMILK*KSYKYT

Sequence NM_001006617

TCCGGAACGCCGGGGTGTGGTTCCGGGTCGTGTGCGGCTCGGGGTAATAGGGCTGCTGCTCGGCCGGCCGGCGGCGGCGAGCAGCAGGGGCATGAGGGCT AACCCGGGAAGCGGCAGCTGAGCGGGCCGGGAGGAGCGCCGGTCCCCGTGGATCCCGAGAGTGCAGAGCTCGGGGCAGGGGCCGGGAGGCGTGGGGGAGC CGGGCCCTCCCCTCAGGAACGTGTCCCGGGGCCGACCCGGCCCGTAGTGTGGAAGCAGCTTCAGGTAGGTGAGCTCGTGAAACAATATGAAGAGGAGAAA ATAGCCTTTTAAGGAAATTGGCCCACAGAAAGGATGGCCTTCTTGGACAATCCAACTATCATTCTAGCTCATATTCGACAGTCACATGTGACCAGTGATG ACACGGGAATGTGTGAGATGGTTCTCATTGATCATGATGTTGACCTAGAGAAGATTCATCCTCCTTCAATGCCTGGAGACAGTGGGTCAGAAATTCAGGG AAGCAATGGTGAGACTCAGGGCTATGTATATGCCCAGTCAGTCGATATTACCTCAAGTTGGGACTTTGGTATTAGAAGACGCTCAAACACAGCTCAAAGA TTAGAACGACTCCGAAAAGAGAGACAAAACCAGATCAAATGCAAAAATATTCAGTGGAAAGAAAGAAATTCTAAGCAATCAGCCCAGGAGTTAAAGTCAC TGTTTGAAAAAAAATCTCTCAAAGAGAAGCCTCCAATTTCTGGGAAGCAGTCGATATTATCTGTACGCCTAGAACAGTGCCCTCTGCAGCTGAATAACCC TTTTAACGAGTATTCCAAATTTGATGGCAAGGGTCATGTAGGTACAACAGCAACCAAGAAGATCGATGTCTACCTCCCTCTGCACTCGAGCCAGGACAGA CTGCTGCCAATGACCGTGGTGACAATGGCCAGCGCCAGGGTGCAGGACCTGATCGGGCTCATCTGCTGGCAGTATACAAGCGAAGGACGGGAGCCGAAGC TCAATGACAATGTCAGTGCCTACTGCCTGCATATTGCTGAGGATGATGGGGAGGTGGACACCGATTTCCCCCCGCTGGATTCCAATGAGCCCATTCATAA GTTTGGCTTCAGTACTTTGGCCCTGGTTGAAAAGTACTCATCTCCTGGTCTGACATCCAAAGAGTCACTCTTTGTTCGAATAAATGCTGCTCATGGATTC TCCCTTATTCAGGTGGACAACACAAAGGTTACCATGAAGGAAATCTTACTGAAGGCAGTGAAGCGAAGAAAAGGATCCCAGAAAGTTTCAGGCCCTCAGT ACCGCCTGGAGAAGCAGAGCGAGCCCAATGTCGCCGTTGACCTGGACAGCACTTTGGAGAGCCAGAGCGCATGGGAGTTCTGCCTGGTCCGCGAGAACAG TTCAAGGGCAGACGGGGTTTTTGAGGAGGATTCGCAAATTGACATAGCCACAGTACAGGATATGCTTAGCAGCCACCATTACAAGTCATTCAAAGTCAGC ATGATCCACAGACTGCGATTCACAACCGACGTACAGCTAGGTATCTCTGGAGACAAAGTAGAGATAGACCCTGTTACGAATCAGAAAGCCAGCACTAAGT TTTGGATTAAGCAGAAACCCATCTCAATCGATTCCGACCTGCTCTGTGCCTGTGACCTTGCTGAAGAGAAAAGCCCCAGTCACGCAATATTTAAACTCAC GTATCTAAGCAATCACGACTATAAACACCTCTACTTTGAATCGGACGCTGCTACCGTCAATGAAATTGTGCTCAAGGTTAACTACATCCTGGAATCGCGA GCTAGCACTGCCCGGGCTGACTACTTTGCTCAAAAACAAAGAAAACTGAACAGACGTACGAGCTTCAGCTTCCAGAAGGAGAAGAAATCCGGGCAGCAGT GACACTGGCCTCCAGCCTCAATCTGTTCCGTAGCTCAGAGCCTGCCTGCCAGGGCCAAGTGCCCTAGAGTCCACCCGGTGTCCTGAAGTCCTCGGGGGGA GGCCAGCCCCTGGCTCACTGGCACAGGGCAGGTGGGCTCTCGGGGAAGGTGTCGGGGGCCCCCTAGGAGGGAGCGCTGGGGACATTGCCATGGGACGGAA GTCTGCTTGGCAGTGGCTTTGATAAGCGATGCTTGGGGGTCAGACCACCCCCTAGAGGAGCCACGTGCCGCCCAGCCACCTTCAATGCCTGCCACCCTGC CCGAGGATGTACAGAGCCGTGCCCACACATTTCCTTGCAACTTGATCAAATTTCTTAAAGCAAACAACAAAAATGTACATTTCTGTTTTTCCTTTTAATA AACAGGTGTACTCTTTATCATGGTTGGTATGATGGACCATTCTTTGGGGCGGAGGATTGATTATGTTACTCTCTTTAAAATCTGTTCCCATATTGAACAG GCAGATTGGAAAAGCTATGGTTCGATTTCTCAGAAGAAATGTTTAGGTCTTAGTCAATAGTTTTAACTATGCCATTTGTTTAAATGAGTGCATTTGCTTC GAGGGTAGTGTCTTACTAAAAGTTAGGAACAGAGACCTAGTGGTGTGTCCAAGGCCGTGTCACTTTCCCCTTCAGCACACCCCAGCTTCTGACCTCAGAG CCCAGGAGCTGCGTGGACAGTGTGGGGTGCCAGGAGGAGGGGCGGTGGCTGGTCCTCAGGCACGCTGCACTCCCAGCCAGACATGGTCTTTCCGTTTCTT AAGTAGCAAGTGTAGGTTTCAGCTGGCAGTTCCACCTGCATGTTCTCTGCTTCGCTGCCTTGGAAGGGGCCACATTCCCCATTCCTCTTCTCCTTACAGC GCCTGCCTCCTTTTTCAAGCAGGCGGAAAGCTGCTGTTTCTCACGTTTCAGGGAGAGGGGTGAGCGGAGGGAGACCTGTGTCCGTGCCGTCCGGCTCCCT GGGTGGGAACAGGCAAGGGATCAGATGCCCCTGACACCACGCCTCTGGCCACACCAGATGCCTCTGCAGTCCTCGACAGCCTCTTCAGTGTCCCTCCTGC GGTGATGTCCTTACTGTCCCCAGCCAGGGCCGGGGACCGGTGTTTCACTGAGGACCTGCATTAGAAACATTTTTTAAATTGTTGTACAGGAAGAGATGTG TCTAAAACAGCATCTTAAAGCTGAGTGTATTTCTTTGCACAAGGGGTCATGCTGATGAATTCTTCTTTCATTCTGATCTTTGTTCAGCCAACAGGAGCGT CCTTTTCTAATGTCTTCCATTCCTACCCCCCACCCAAAAACAAAAGAAATATTTGTAGCTTGCTATCTGTATTTGAATTTTTAGCAATTTTATATTTAGA TACTTTGAAAAATGTAAATGACTAATTTGGTCATTAAATCTTGTGACATATTCGATATTAAAATGATATTAAAATAAAAGTCATATAAATACACA

refseq_MAPKAP1.F5 (forward primer)
refseq_MAPKAP1.R5 (reverse primer)
Single exon skipping

Frame 0

SGTPGCGSGSCAARGNRAAARPAGGGEQQGHEG *PGKRQLSGPGGAPVPVDPESAELGAGAGRRGGA GPSPQERVPGPTRPVVWKQLQVGELVKQYEEEK IAF*GNWPTERMAFLDNPTIILAHIRQSHVTSD DTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQG SNGETQGYVYAQSVDITSSWDFGIRRRSNTAQR LERLRKERQNQIKCKNIQWKERNSKQSAQELKS LFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNP FNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDR LLPMTVVTMASARVQDLIGLICWQYTSEGREPK LNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHK FGFSTLALVEKYSSPGLTSKESLFVRINAAHGF SLIQVDNTKVTMKEILLKAVKRRKGSQKVSGPQ YRLEKQSEPNVAVDLDSTLESQSAWEFCLVRENS SRADGVFEEDSQIDIATVQDMLSSHHYKSFKVS MIHRLRFTTDVQLGISGDKVEIDPVTNQKASTK FWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLT YLSNHDYKHLYFESDAATVNEIVLKVNYILESR ASTARADYFAQKQRKLNRRTSFSFQKEKKSGQQ *HWPPASICSVAQSLPARAKCPRVHPVS*SPRGE ASPWLTGTGQVGSRGRCRGPPRRERWGHCHGTE VCLAVALISDAWGSDHPLEEPRAAQPPSMPATL PEDVQSRAHTFPCNLIKFLKANNKNVHFCFSF** TGVLFIMVGMMDHSLGRRIDYVTLFKICSHIEQ ADWKSYGSISQKKCLGLSQ*F*LCHLFK*VHLL RG*CLTKS*EQRPSGVSKAVSLSPSAHPSF*PQS PGAAWTVWGARRRGGGWSSGTLHSQPDMVFPFL K*QV*VSAGSSTCMFSASLPWKGPHSPFLFSLQ RLPPFSSRRKAAVSHVSGRGVSGGRPVSVPSGSL GGNRQGIRCP*HHASGHTRCLCSPRQPLQCPSC GDVLTVPSQGRGPVFH*GPALETFFKLLYRKRC V*NSILKLSVFLCTRGHADEFFFHSDLCSANRSV LF*CLPFLPPTQKQKKYL*LAICI*IFSNFIFR YFEKCK*LIWSLNLVTYSILK*Y*NKSHINT

Frame 1

PERRGVVPGRVRLGVIGLLLGRPAAASSRGMRA NPGSGS*AGREERRSPWIPRVQSSGQGPGGVGE PGPPLRNVSRGRPGP*CGSSFR*VSS*NNMKRRK *PFKEIGPQKGWPSWTIQLSF*LIFDSHM*PVM TRECVRWFSLIMMLT*RRFILLQCLETVGQKFR EAMVRLRAMYMPSQSILPQVGTLVLEDAQTQLKD *NDSEKRDKTRSNAKIFSGKKEILSNQPRS*SH CLKKNLSKRSLQFLGSSRYYLYA*NSALCS*IT LLTSIPNLMARVM*VQQQPRRSMSTSLCTRARTD CCQ*PW*QWPAPGCRT*SGSSAGSIQAKDGSRS SMTMSVPTACILLRMMGRWTPISPRWIPMSPFI SLASVLWPWLKSTHLLV*HPKSHSLFE*MLLMDS PLFRWTTQRLP*RKSY*RQ*SEEKDPRKFQALS TAWRSRASPMSPLTWTALWRARAHGSSAWSART VQGQTGFLRRIRKLT*PQYRICLAATITSHSKSA *STDCDSQPTYS*VSLETK*R*TLLRIRKPALS FGLSRNPSQSIPTCSVPVTLLKRKAPVTQYLNS RI*AITTINTSTLNRTLLPSMKLCSRLTTSWNRE LALPGLTTLLKNKEN*TDVRASASRRRRNPGSS DTGLQPQSVP*LRACLPGPSALESTRCPEVLGG RPAPGSLAQGRWALGEGVGGPLGGSAGDIAMGRK SAWQWL**AMLGGQTTP*RSHVPPSHLQCLPPC PRMYRAVPTHFLAT*SNFLKQTTKMYISVFPFN KQVYSLSWLV*WTILWGGGLIMLLSLKSVPILNR QIGKAMVRFLRRNV*VLVNSFNYAICLNECICF EGSVLLKVRNRDLVVCPRPCHFPLQHTPASDLR AQELRGQCGVPGGGAVAGPQARCTPSQTWSFRFL SSKCRFQLAVPPACSLLRCLGRGHIPHSSSPYS ACLLFQAGGKLLFLTFQGEG*AEGDLCPCRPAP WVGTGKGSDAPDTTPLATPDASAVLDSLFSVPPA VMSLLSPARAGDRCFTEDLH*KHFLNCCTGRDV SKTAS*S*VYFFAQGVMLMNSSFILIFVQPTGA SFSNVFHSYPPPKNKRNICSLLSVFEFLAILYLD TLKNVND*FGH*IL*HIRY*NDIKIKVI*IH

Frame 2

RNAGVWFRVVCGSG**GCCSAGRRRRAAGA*GL TREAAAERAGRSAGPRGSRECRARGRGREAWGS RALPSGTCPGADPARSVEAASGR*ARETI*RGE NSLLRKLAHRKDGLLGQSNYHSSSYSTVTCDQ** HGNV*DGSH*S*C*PREDSSSFNAWRQWVRNSG KQW*DSGLCICPVSRYYLKLGLWY*KTLKHSSK IRTTPKRETKPDQMQKYSVERKKF*AISPGVKVT V*KKISQREASNFWEAVDIICTPRTVPSAAE*P F*RVFQI*WQGSCRYNSNQEDRCLPPSALEPGQ TAANDRGDNGQRQGAGPDRAHLLAVYKRRTGAEA Q*QCQCLLPAYC*G*WGGGHRFPPAGFQ*AHS* VWLQYFGPG*KVLISWSDIQRVTLCSNKCCSWI LPYSGGQHKGYHEGNLTEGSEAKKRIPESFRPSV PPGEAERAQCRR*PGQHFGEPERMGVLPGPREQ FKGRRGF*GGFAN*HSHSTGYA*QPPLQVIQSQ HDPQTAIHNRRTARYLWRQSRDRPCYESESQH*V LD*AETHLNRFRPALCL*PC*REKPQSRNI*TH VSKQSRL*TPLL*IGRCYRQ*NCAQG*LHPGIA S*HCPG*LLCSKTKKTEQTYELQLPEGEEIRAAV TLASSLNLFRSSEPACQGQVP*SPPGVLKSSGG GQPLAHWHRAGGLSGKVSGAP*EGALGTLPWDG SLLGSGFDKRCLGVRPPPRGATCRPATFNACHPA RGCTEPCPHISLQLDQIS*SKQQKCTFLFFLLI NRCTLYHGWYDGPFFGAED*LCYSL*NLFPY*T GRLEKLWFDFSEEMFRS*SIVLTMPFV*MSAFAS RVVSY*KLGTET*WCVQGRVTFPFSTPQLLTSE PRSCVDSVGCQEEGRWLVLRHAALPARHGLSVS *VASVGFSWQFHLHVLCFAALEGATFPIPLLLTA PASFFKQAESCCFSRFRERGERRETCVRAVRLP GWEQARDQMPLTPRLWPHQMPLQSSTASSVSLL R*CPYCPQPGPGTGVSLRTCIRNIF*IVVQEEMC LKQHLKAECISLHKGSC**ILLSF*SLFSQQER PFLMSSIPTPHPKTKEIFVACYLYLNF*QFYI* IL*KM*MTNLVIKSCDIFDIKMILK*KSYKYT