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Sequence NM_181702
CGGCGGCTGCAGGCGGCGGGCAAGGACGGCGGGCACAGCGCAGCACTCCCCGCTCGTTGGCCCGGGTATCCCAGCGCGGACCCACGCGATACGCTGACGCCCCGACGCCGATCCGGCCGAGCCAAGACTCAACGATGACTCTGAATAATGTCACCATGCGCCAGGGCACTGTGGGCATGCAGCCACAGCAGCAGCGCTGG
AGCATCCCAGCTGATGGCAGGCATCTGATGGTCCAGAAAGAGCCCCACCAGTACAGCCACCGCAACCGCCATTCTGCTACCCCTGAGGACCACTGCCGCC
GAAGCTGGTCCTCTGACTCCACAGACTCAGTCATCTCCTCTGAGTCAGGGAACACCTACTACCGAGTGGTGCTCATAGGGGAGCAGGGGGTGGGCAAGTC
CACTCTGGCCAACATCTTTGCAGGTGTGCATGACAGCATGGACAGCGACTGCGAGGTGCTGGGAGAAGATACATATGAACGAACCCTGATGGTTGATGGG
GAAAGTGCAACGATTATACTCCTGGATATGTGGGAAAATAAGGGGGAAAATGAATGGCTCCATGACCACTGCATGCAGGTCGGGGACGCATACCTGATTG
TCTACTCAATCACAGACCGAGCGAGCTTCGAGAAGGCATCTGAGCTGCGAATCCAGCTCCGCAGGGCCCGGCAGACAGAGGACATTCCCATAATTTTGGT
TGGCAACAAAAGTGACTTAGTGCGGTGCCGAGAAGTGTCTGTATCAGAAGGGAGAGCCTGTGCAGTGGTGTTTGACTGCAAGTTCATCGAGACCTCTGCA
GCTGTCCAGCACAACGTGAAGGAGCTGTTTGAGGGCATTGTGCGACAGGTGCGCCTTCGGCGGGACAGCAAGGAGAAGAATGAACGGCGGCTGGCCTACC
AGAAAAGGAAGGAGAGCATGCCCAGGAAAGCCAGGCGCTTCTGGGGCAAGATCGTGGCCAAAAACAACAAGAATATGGCCTTCAAGCTCAAGTCCAAATC
CTGCCATGACCTCTCTGTACTCTAGGAACCCAGGGTCACCCAGATGTCCCTTTGATGGCCGTTGTTGAAGGCCATTGGGACCAATAATCTATATTAGATT
GAATACTTAAGTTAGATGTGGTTTCCCCCATTGTAGCAGGGAGCTAGCGTATTAGCCTTGTGGGCAACATGATGCATGGGAAATGAAAGATTTTTGTAAA
AAGTCAGTATTTATTTCCAGGAAAAGCCTGACCTTGCTATTTGAACACCCAAGACTCTTTAGAGGATGTGTTTGGTGTTCACATGTGTTTCTTCTATTTT
GGATAGTAGAGAAGTAAAGCTTACAAAGAATGCCTAGAACAAGAACTTTTCATCATTAAAAATTTTTCCCAGTGTTCTGATATGTGACTTTGAGGCCAAT
GAGTCATAAACAAATATAAGAAAGCTGTCAATGAGTTTCTTCAAAGGAGGGAAAACTTTCTACGAATCTAAGATCCATGGAGCTAGAATTGTAGAACTAG
GCTCATCAGAATCGTGACTATTATTGCTCCATCAAACTGTGAAAAGAAATGATGTGGACCTTGCTGGAAACAAAGGCTTAGCAAACAATTTTTGTTCAAT
GCCCACCAAGACATATAGAATTGGGAACTGATACATGTGTCCCTTATAGGCTCAAAAATTATATCTTACAATTTCTTATTTAGGGGGAAATTATTTGAAT
CAGATTCTATTTAGTCAAACCACCTTTTATGTTTTATTATTTTTGAATTCATGGAGCCATCATAAAAATATTTTTAAAATCAGAATTATTGATACCCTGT
AGTGCAAAATGTCAATTTTTAATGTATAATCAGAAGTCTGAATTTTTATAAAACATATAGCATAAAAACTTCCAGTACTTTGGTTGACCCTTGTATGTCA
CAGCTCTGCTCTATTTATTATTATTTTGCAAAATAACCATTTTAACATTTGATAAAGCATATTTATGAACATATTTCTTAATAAGAAAAATATCCATTTT
ATTACCATTTTCTATCTTTTTCAAAATATGCAAGTTTTTACCTATATGTCTTATAATAAAAGAAATAAAATATTTGAAAAAA
- refseq_GEM.F2 (forward primer)
- refseq_GEM.R2 (reverse primer)
- Alternative donor
Frame 0
RRLQAAGKDGGHSAALPARWPGYPSADPRDTLTPRRRSGRAKTQR*L*IMSPCARALWACSHSSSAG
ASQLMAGI*WSRKSPTSTATATAILLPLRTTAA
EAGPLTPQTQSSPLSQGTPTTEWCS*GSRGWAS
PLWPTSLQVCMTAWTATARCWEKIHMNEP*WLMG
KVQRLYSWICGKIRGKMNGSMTTACRSGTHT*L
STQSQTERASRRHLSCESSSAGPGRQRTFP*FW
LATKVT*CGAEKCLYQKGEPVQWCLTASSSRPLQ
LSSTT*RSCLRALCDRCAFGGTARRRMNGGWPT
RKGRRACPGKPGASGARSWPKTTRIWPSSSSPN
PAMTSLYSRNPGSPRCPFDGRC*RPLGPIIYIRL
NT*VRCGFPHCSRELAY*PCGQHDAWEMKDFCK
KSVFISRKSLTLLFEHPRLFRGCVWCSHVFLLF
WIVEK*SLQRMPRTRTFHH*KFFPVF*YVTLRPM
SHKQI*ESCQ*VSSKEGKLSTNLRSMELEL*N*
AHQNRDYYCSIKL*KEMMWTLLETKA*QTIFVQ
CPPRHIELGTDTCVPYRLKNYILQFLI*GEII*I
RFYLVKPPFMFYYF*IHGAIIKIFLKSELLIPC
SAKCQFLMYNQKSEFL*NI*HKNFQYFG*PLYV
TALLYLLLFCKITILTFDKAYL*TYFLIRKISIL
LPFSIFFKICKFLPICLIIKEIKYLKK
Frame 1
GGCRRRARTAGTAQHSPLVGPGIPARTHAIR*RPDADPAEPRLNDDSE*CHHAPGHCGHAATAAAL
EHPS*WQASDGPERAPPVQPPQPPFCYP*GPLPP
KLVL*LHRLSHLL*VREHLLPSGAHRGAGGGQV
HSGQHLCRCA*QHGQRLRGAGRRYI*TNPDG*W
GKCNDYTPGYVGK*GGK*MAP*PLHAGRGRIPDC
LLNHRPSELREGI*AANPAPQGPADRGHSHNFG
WQQK*LSAVPRSVCIRRESLCSGV*LQVHRDLC
SCPAQREGAV*GHCATGAPSAGQQGEE*TAAGLP
EKEGEHAQESQALLGQDRGQKQQEYGLQAQVQI
LP*PLCTLGTQGHPDVPLMAVVEGHWDQ*SILD
*ILKLDVVSPIVAGS*RISLVGNMMHGK*KIFVK
SQYLFPGKA*PCYLNTQDSLEDVFGVHMCFFYF
G**RSKAYKECLEQELFIIKNFSQCSDM*L*GQ
*VINKYKKAVNEFLQRRENFLRI*DPWS*NCRTR
LIRIVTIIAPSNCEKK*CGPCWKQRLSKQFLFN
AHQDI*NWELIHVSLIGSKIISYNFLFRGKLFE
SDSI*SNHLLCFIIFEFMEPS*KYF*NQNY*YPV
VQNVNF*CIIRSLNFYKTYSIKTSSTLVDPCMS
QLCSIYYYFAK*PF*HLIKHIYEHIS**EKYPF
YYHFLSFSKYASFYLYVL**KK*NI*K
Frame 2
AAAGGGQGRRAQRSTPRSLARVSQRGPTRYADAPTPIRPSQDSTMTLNNVTMRQGTVGMQPQQQRW
SIPADGRHLMVQKEPHQYSHRNRHSATPEDHCR
RSWSSDSTDSVISSESGNTYYRVVLIGEQGVGKS
TLANIFAGVHDSMDSDCEVLGEDTYERTLMVDG
ESATIILLDMWENKGENEWLHDHCMQVGDAYLI
VYSITDRASFEKASELRIQLRRARQTEDIPIILV
GNKSDLVRCREVSVSEGRACAVVFDCKFIETSA
AVQHNVKELFEGIVRQVRLRRDSKEKNERRLAY
QKRKESMPRKARRFWGKIVAKNNKNMAFKLKSKS
CHDLSVL*EPRVTQMSL*WPLLKAIGTNNLY*I
EYLS*MWFPPL*QGASVLALWAT*CMGNERFL*
KVSIYFQEKPDLAI*TPKTL*RMCLVFTCVSSIL
DSREVKLTKNA*NKNFSSLKIFPSVLICDFEAN
ES*TNIRKLSMSFFKGGKTFYESKIHGARIVEL
GSSES*LLLLHQTVKRNDVDLAGNKGLANNFCSM
PTKTYRIGN*YMCPL*AQKLYLTISYLGGNYLN
QILFSQTTFYVLLFLNSWSHHKNIFKIRIIDTL
*CKMSIFNV*SEV*IFIKHIA*KLPVLWLTLVCH
SSALFIIILQNNHFNI**SIFMNIFLNKKNIHF
ITIFYLFQNMQVFTYMSYNKRNKIFEK
Sequence NM_005261
CGGCGGCTGCAGGCGGCGGGCAAGGACGGCGGGCACAGCGCAGCACTCCCCGCTCGTTGGCCCGGGTATCCCAGCGCGGACCCACGCGATACGCTGACGCCCCGACGCCGATCCGGCCGAGCCAAGTAAGGGGGACGGCCCGAGACGGAGAAGGGAGAGAGTGGGAGTTTCCCAGCCCGCAGAACTTTCGAAGTTGAGAA
AAGAACCCCTGGAACGTGCGCTCAGCACTGGGATTTTCTGGACTCAACGATGACTCTGAATAATGTCACCATGCGCCAGGGCACTGTGGGCATGCAGCCA
CAGCAGCAGCGCTGGAGCATCCCAGCTGATGGCAGGCATCTGATGGTCCAGAAAGAGCCCCACCAGTACAGCCACCGCAACCGCCATTCTGCTACCCCTG
AGGACCACTGCCGCCGAAGCTGGTCCTCTGACTCCACAGACTCAGTCATCTCCTCTGAGTCAGGGAACACCTACTACCGAGTGGTGCTCATAGGGGAGCA
GGGGGTGGGCAAGTCCACTCTGGCCAACATCTTTGCAGGTGTGCATGACAGCATGGACAGCGACTGCGAGGTGCTGGGAGAAGATACATATGAACGAACC
CTGATGGTTGATGGGGAAAGTGCAACGATTATACTCCTGGATATGTGGGAAAATAAGGGGGAAAATGAATGGCTCCATGACCACTGCATGCAGGTCGGGG
ACGCATACCTGATTGTCTACTCAATCACAGACCGAGCGAGCTTCGAGAAGGCATCTGAGCTGCGAATCCAGCTCCGCAGGGCCCGGCAGACAGAGGACAT
TCCCATAATTTTGGTTGGCAACAAAAGTGACTTAGTGCGGTGCCGAGAAGTGTCTGTATCAGAAGGGAGAGCCTGTGCAGTGGTGTTTGACTGCAAGTTC
ATCGAGACCTCTGCAGCTGTCCAGCACAACGTGAAGGAGCTGTTTGAGGGCATTGTGCGACAGGTGCGCCTTCGGCGGGACAGCAAGGAGAAGAATGAAC
GGCGGCTGGCCTACCAGAAAAGGAAGGAGAGCATGCCCAGGAAAGCCAGGCGCTTCTGGGGCAAGATCGTGGCCAAAAACAACAAGAATATGGCCTTCAA
GCTCAAGTCCAAATCCTGCCATGACCTCTCTGTACTCTAGGAACCCAGGGTCACCCAGATGTCCCTTTGATGGCCGTTGTTGAAGGCCATTGGGACCAAT
AATCTATATTAGATTGAATACTTAAGTTAGATGTGGTTTCCCCCATTGTAGCAGGGAGCTAGCGTATTAGCCTTGTGGGCAACATGATGCATGGGAAATG
AAAGATTTTTGTAAAAAGTCAGTATTTATTTCCAGGAAAAGCCTGACCTTGCTATTTGAACACCCAAGACTCTTTAGAGGATGTGTTTGGTGTTCACATG
TGTTTCTTCTATTTTGGATAGTAGAGAAGTAAAGCTTACAAAGAATGCCTAGAACAAGAACTTTTCATCATTAAAAATTTTTCCCAGTGTTCTGATATGT
GACTTTGAGGCCAATGAGTCATAAACAAATATAAGAAAGCTGTCAATGAGTTTCTTCAAAGGAGGGAAAACTTTCTACGAATCTAAGATCCATGGAGCTA
GAATTGTAGAACTAGGCTCATCAGAATCGTGACTATTATTGCTCCATCAAACTGTGAAAAGAAATGATGTGGACCTTGCTGGAAACAAAGGCTTAGCAAA
CAATTTTTGTTCAATGCCCACCAAGACATATAGAATTGGGAACTGATACATGTGTCCCTTATAGGCTCAAAAATTATATCTTACAATTTCTTATTTAGGG
GGAAATTATTTGAATCAGATTCTATTTAGTCAAACCACCTTTTATGTTTTATTATTTTTGAATTCATGGAGCCATCATAAAAATATTTTTAAAATCAGAA
TTATTGATACCCTGTAGTGCAAAATGTCAATTTTTAATGTATAATCAGAAGTCTGAATTTTTATAAAACATATAGCATAAAAACTTCCAGTACTTTGGTT
GACCCTTGTATGTCACAGCTCTGCTCTATTTATTATTATTTTGCAAAATAACCATTTTAACATTTGATAAAGCATATTTATGAACATATTTCTTAATAAG
AAAAATATCCATTTTATTACCATTTTCTATCTTTTTCAAAATATGCAAGTTTTTACCTATATGTCTTATAATAAAAGAAATAAAATATTTGAAAAAA
- refseq_GEM.F2 (forward primer)
- refseq_GEM.R2 (reverse primer)
- Alternative donor
Frame 0
RRLQAAGKDGGHSAALPARWPGYPSADPRDTLTPRRRSGRAK*GGRPETEKGESGSFPARRTFEVEK
RTPGTCAQHWDFLDSTMTLNNVTMRQGTVGMQP
QQQRWSIPADGRHLMVQKEPHQYSHRNRHSATP
EDHCRRSWSSDSTDSVISSESGNTYYRVVLIGEQ
GVGKSTLANIFAGVHDSMDSDCEVLGEDTYERT
LMVDGESATIILLDMWENKGENEWLHDHCMQVG
DAYLIVYSITDRASFEKASELRIQLRRARQTEDI
PIILVGNKSDLVRCREVSVSEGRACAVVFDCKF
IETSAAVQHNVKELFEGIVRQVRLRRDSKEKNE
RRLAYQKRKESMPRKARRFWGKIVAKNNKNMAFK
LKSKSCHDLSVL*EPRVTQMSL*WPLLKAIGTN
NLY*IEYLS*MWFPPL*QGASVLALWAT*CMGN
ERFL*KVSIYFQEKPDLAI*TPKTL*RMCLVFTC
VSSILDSREVKLTKNA*NKNFSSLKIFPSVLIC
DFEANES*TNIRKLSMSFFKGGKTFYESKIHGA
RIVELGSSES*LLLLHQTVKRNDVDLAGNKGLAN
NFCSMPTKTYRIGN*YMCPL*AQKLYLTISYLG
GNYLNQILFSQTTFYVLLFLNSWSHHKNIFKIR
IIDTL*CKMSIFNV*SEV*IFIKHIA*KLPVLWL
TLVCHSSALFIIILQNNHFNI**SIFMNIFLNK
KNIHFITIFYLFQNMQVFTYMSYNKRNKIFEK
Frame 1
GGCRRRARTAGTAQHSPLVGPGIPARTHAIR*RPDADPAEPSKGDGPRRRRERVGVSQPAELSKLR
KEPLERALSTGIFWTQR*L*IMSPCARALWACSH
SSSAGASQLMAGI*WSRKSPTSTATATAILLPL
RTTAAEAGPLTPQTQSSPLSQGTPTTEWCS*GS
RGWASPLWPTSLQVCMTAWTATARCWEKIHMNEP
*WLMGKVQRLYSWICGKIRGKMNGSMTTACRSG
THT*LSTQSQTERASRRHLSCESSSAGPGRQRT
FP*FWLATKVT*CGAEKCLYQKGEPVQWCLTASS
SRPLQLSSTT*RSCLRALCDRCAFGGTARRRMN
GGWPTRKGRRACPGKPGASGARSWPKTTRIWPS
SSSPNPAMTSLYSRNPGSPRCPFDGRC*RPLGPI
IYIRLNT*VRCGFPHCSRELAY*PCGQHDAWEM
KDFCKKSVFISRKSLTLLFEHPRLFRGCVWCSH
VFLLFWIVEK*SLQRMPRTRTFHH*KFFPVF*YV
TLRPMSHKQI*ESCQ*VSSKEGKLSTNLRSMEL
EL*N*AHQNRDYYCSIKL*KEMMWTLLETKA*Q
TIFVQCPPRHIELGTDTCVPYRLKNYILQFLI*G
EII*IRFYLVKPPFMFYYF*IHGAIIKIFLKSE
LLIPCSAKCQFLMYNQKSEFL*NI*HKNFQYFG
*PLYVTALLYLLLFCKITILTFDKAYL*TYFLIR
KISILLPFSIFFKICKFLPICLIIKEIKYLKK
Frame 2
AAAGGGQGRRAQRSTPRSLARVSQRGPTRYADAPTPIRPSQVRGTARDGEGREWEFPSPQNFRS*E
KNPWNVRSALGFSGLNDDSE*CHHAPGHCGHAA
TAAALEHPS*WQASDGPERAPPVQPPQPPFCYP*
GPLPPKLVL*LHRLSHLL*VREHLLPSGAHRGA
GGGQVHSGQHLCRCA*QHGQRLRGAGRRYI*TN
PDG*WGKCNDYTPGYVGK*GGK*MAP*PLHAGRG
RIPDCLLNHRPSELREGI*AANPAPQGPADRGH
SHNFGWQQK*LSAVPRSVCIRRESLCSGV*LQV
HRDLCSCPAQREGAV*GHCATGAPSAGQQGEE*T
AAGLPEKEGEHAQESQALLGQDRGQKQQEYGLQ
AQVQILP*PLCTLGTQGHPDVPLMAVVEGHWDQ
*SILD*ILKLDVVSPIVAGS*RISLVGNMMHGK*
KIFVKSQYLFPGKA*PCYLNTQDSLEDVFGVHM
CFFYFG**RSKAYKECLEQELFIIKNFSQCSDM
*L*GQ*VINKYKKAVNEFLQRRENFLRI*DPWS*
NCRTRLIRIVTIIAPSNCEKK*CGPCWKQRLSK
QFLFNAHQDI*NWELIHVSLIGSKIISYNFLFR
GKLFESDSI*SNHLLCFIIFEFMEPS*KYF*NQN
Y*YPVVQNVNF*CIIRSLNFYKTYSIKTSSTLV
DPCMSQLCSIYYYFAK*PF*HLIKHIYEHIS**
EKYPFYYHFLSFSKYASFYLYVL**KK*NI*K
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