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Sequences

Sequence NM_001002879

GGGCAGTGGGGCTGGGAGATGGGGCGGGGCTGGGCGCTCCGGGACTGAGGTGCTGATCGCCCAAAGGGCGCCCCTGGGCTGGGGTCTGACCCGGCTTCTC
CTCCCTCACTACCCGCGGTCCCCGGCCTCTCGGCGCTGCGCTGTTACCTCATCCGAAAAGTGTTCGCTGCAGCATCCTCAGGCTTCACGTTGGAGCCTCT
TGATTGTGGAGGAACAACCATGTCATCAGAATCGAGCAAAAAACGGAAGCCCAAAGTGATCCGAAGCGATGGAGCCCCAGCTGAAGGAAAGCGGAATCGA
TCTGACACCGAGCAGGAAGGTAAATACTACAGTGAGGAGGCCGAGGTGGATCTGCGGGACCCTGGCAGAGACTATGAGTTATACAAGTACACCTGCCAGG
AGCTACAGAGGCTGATGGCTGAGATCCAAGACCTGAAGAGCAGGGGTGGCAAGGATGTGGCAATAGAAATAGAAGAACGGAGGATCCAGAGCTGTGTGCA
TTTCATGACTCTAAAGAAGCTTAACCGATTAGCCCACATCAGGTTGAAGAAAGGAAGAGATCAGACCCACGAGGCTAAGCAGAAAGTAGATGCCTATCAT
CTGCAGCTCCAGAACCTGTTGTATGAGGTGATGCACCTACAGAAGGAGATCACCAAATGTTTGGAGTTTAAGTCAAAGCATGAAGAAATTGATCTGGTCA
GTTTAGAGGAGTTTTATAAGGAGGCTCCACCAGATATCAGCAAGGCCGAAGTCACCATGGGAGACCCTCACCAGCAAACACTGGCACGTCTGGACTGGGA
GCTGGAGCAGCGGAAAAGGCTGGCAGAGAAGTACCGAGAGTGCCTATCTAACAAGGAGAAGATTCTCAAGGAGATTGAGGTGAAGAAGGAGTACCTGAGC
AGCCTCCAGCCCCGCCTCAACAGCATCATGCAGGCTTCCCTTCCGGTGCAGGAGTACCTGTTTATGCCATTCGACCAGGCTCACAAGCAGTATGAGACAG
CCAGACACCTGCCGCCTCCCCTCTATGTCCTCTTTGTTCAGGCCACTGCGTATGGGCAGGCCTGTGATAAGACGTTATCTGTGGCAATCGAAGGCAGTGT
GGATGAAGCCAAGGCTCTGTTCAAACCTCCAGAGGACTCCCAAGATGACGAGAGTGACTCAGATGCCGAGGAGGAGCAGACTACGAAGCGCCGGAGACCC
ACACTGGGGGTTCAGTTGGACGACAAACGCAAGGAGATGCTGAAGAGGCACCCACTGTCTGTCATGCTCGACCTGAAGTGCAAAGATGACAGTGTGCTTC
ACCTGACTTTCTACTACCTCATGAACCTCAACATCATGACAGTAAAAGCCAAAGTGACAACTGCCATGGAGCTGATCACCCCCATCAGTGCAGGTGACTT
GCTGTCTCCTGACTCAGTCCTGAGTTGCTTGTATCCTGGGGATCATGGAAAGAAAACTCCGAATCCAGCCAATCAGTATCAGTTTGATAAAGTTGGCATC
CTGACTTTGAGCGACTATGTACTTGAGCTAGGTCACCCCTATTTGTGGGTGCAGAAGCTGGGTGGCCTCCACTTCCCCAAAGAGCAGCCCCAGCAAACAG
TGATTGCTGACCACTCGCTGAGCGCCAGCCACATGGAGACCACCATGAAACTTCTGAAGACCAGGGTGCAGTCCCGCCTGGCCCTCCACAAACAGTTTGC
ATCCCTAGAACATGGCATTGTGCCAGTTACCAGTGATTGCCAGTACCTCTTCCCTGCCAAGGTTGTCTCTCGCCTGGTGAAATGGGTGACAGTTGCCCAT
GAGGATTACATGGAGCTGCACTTCACCAAAGACATTGTGGATGCGGGACTGGCTGGGGACACCAATCTCTACTACATGGCGCTCATCGAAAGGGGCACAG
CCAAACTGCAGGCCGCTGTGGTGTTGAACCCTGGCTACTCCTCCATCCCACCTGTTTTCCAGCTCTGTTTGAACTGGAAAGGGGAGAAAACCAACAGCAA
CGATGACAACATTCGGGCCATGGAGGGCGAAGTCAATGTGTGCTACAAGGAGCTGTGTGGCCCTTGGCCCAGCCACCAGCTGTTGACCAACCAGCTGCAG
CGGCTGTGTGTGCTGCTGGATGTTTACCTGGAGACCGAGAGCCATGACGACAGTGTGGAGGGGCCCAAGGAATTTCCCCAGGAGAAGATGTGTCTGCGGC
TCTTCAGGGGTCCTAGCAGGATGAAGCCATTTAAATACAACCATCCTCAGGGATTCTTCAGCCATCGCTGATCTCCCGCGCAGACCGTTGTTTCCCCCAA
GGCCTCACCCTGAGCACTGGGCTTCTGCTTTCTGCTCTGGCCCACATGTGACTCTTGATATTCTCCAAAGACACCAGCCAATTAAAAAGCGTCACCTGAC
CAGTGGCCTTTGTCTGTGGTTCCTGGCAAGGTGGCTTTGCAGTCTGGAAGGGCAGGTGGGAGCTGTGACACAGTGTGAAAAAGCATTTGTAGAGAGACTT
TTTCTCAGCAGCCAATAAAAGCAGAGTGGAAAAAGATTCCAATTCTGCAGAGAGATGCTCA
  • refseq_THOC5.F4 (forward primer)
  • refseq_THOC5.R3 (reverse primer)
  • Single exon skipping

Frame 0

GQWGWEMGRGWALRD*GADRPKGAPGLGSDPAS
PPSLPAVPGLSALRCYLIRKVFAAASSGFTLEPL
DCGGTTMSSESSKKRKPKVIRSDGAPAEGKRNR
SDTEQEGKYYSEEAEVDLRDPGRDYELYKYTCQ
ELQRLMAEIQDLKSRGGKDVAIEIEERRIQSCVH
FMTLKKLNRLAHIRLKKGRDQTHEAKQKVDAYH
LQLQNLLYEVMHLQKEITKCLEFKSKHEEIDLV
SLEEFYKEAPPDISKAEVTMGDPHQQTLARLDWE
LEQRKRLAEKYRECLSNKEKILKEIEVKKEYLS
SLQPRLNSIMQASLPVQEYLFMPFDQAHKQYET
ARHLPPPLYVLFVQATAYGQACDKTLSVAIEGSV
DEAKALFKPPEDSQDDESDSDAEEEQTTKRRRP
TLGVQLDDKRKEMLKRHPLSVMLDLKCKDDSVL
HLTFYYLMNLNIMTVKAKVTTAMELITPISAGDL
LSPDSVLSCLYPGDHGKKTPNPANQYQFDKVGI
LTLSDYVLELGHPYLWVQKLGGLHFPKEQPQQT
VIADHSLSASHMETTMKLLKTRVQSRLALHKQFA
SLEHGIVPVTSDCQYLFPAKVVSRLVKWVTVAH
EDYMELHFTKDIVDAGLAGDTNLYYMALIERGT
AKLQAAVVLNPGYSSIPPVFQLCLNWKGEKTNSN
DDNIRAMEGEVNVCYKELCGPWPSHQLLTNQLQ
RLCVLLDVYLETESHDDSVEGPKEFPQEKMCLR
LFRGPSRMKPFKYNHPQGFFSHR*SPAQTVVSPK
ASP*ALGFCFLLWPTCDS*YSPKTPAN*KASPD
QWPLSVVPGKVALQSGRAGGSCDTV*KSICRET
FSQQPIKAEWKKIPILQRDA

Frame 1

GSGAGRWGGAGRSGTEVLIAQRAPLGWGLTRLL
LPHYPRSPASRRCAVTSSEKCSLQHPQASRWSL
LIVEEQPCHQNRAKNGSPK*SEAMEPQLKESGID
LTPSRKVNTTVRRPRWICGTLAETMSYTSTPAR
SYRG*WLRSKT*RAGVARMWQ*K*KNGGSRAVC
IS*L*RSLTD*PTSG*RKEEIRPTRLSRK*MPII
CSSRTCCMR*CTYRRRSPNVWSLSQSMKKLIWS
V*RSFIRRLHQISARPKSPWETLTSKHWHVWTG
SWSSGKGWQRSTESAYLTRRRFSRRLR*RRST*A
ASSPASTASCRLPFRCRSTCLCHSTRLTSSMRQ
PDTCRLPSMSSLFRPLRMGRPVIRRYLWQSKAV
WMKPRLCSNLQRTPKMTRVTQMPRRSRLRSAGDP
HWGFSWTTNARRC*RGTHCLSCST*SAKMTVCF
T*LSTTS*TSTS*Q*KPK*QLPWS*SPPSVQVT
CCLLTQS*VACILGIMERKLRIQPISISLIKLAS
*L*ATMYLS*VTPICGCRSWVASTSPKSSPSKQ
*LLTTR*APATWRPP*NF*RPGCSPAWPSTNSL
HP*NMALCQLPVIASTSSLPRLSLAW*NG*QLPM
RITWSCTSPKTLWMRDWLGTPISTTWRSSKGAQ
PNCRPLWC*TLATPPSHLFSSSV*TGKGRKPTA
TMTTFGPWRAKSMCATRSCVALGPATSC*PTSCS
GCVCCWMFTWRPRAMTTVWRGPRNFPRRRCVCG
SSGVLAG*SHLNTTILRDSSAIADLPRRPLFPP
RPHPEHWASAFCSGPHVTLDILQRHQPIKKRHLT
SGLCLWFLARWLCSLEGQVGAVTQCEKAFVERL
FLSSQ*KQSGKRFQFCREML

Frame 2

AVGLGDGAGLGAPGLRC*SPKGRPWAGV*PGFS
SLTTRGPRPLGAALLPHPKSVRCSILRLHVGAS
*LWRNNHVIRIEQKTEAQSDPKRWSPS*RKAES
I*HRAGR*ILQ*GGRGGSAGPWQRL*VIQVHLPG
ATEADG*DPRPEEQGWQGCGNRNRRTEDPELCA
FHDSKEA*PISPHQVEERKRSDPRG*AESRCLS
SAAPEPVV*GDAPTEGDHQMFGV*VKA*RN*SGQ
FRGVL*GGSTRYQQGRSHHGRPSPANTGTSGLG
AGAAEKAGREVPRVPI*QGEDSQGD*GEEGVPE
QPPAPPQQHHAGFPSGAGVPVYAIRPGSQAV*DS
QTPAASPLCPLCSGHCVWAGL**DVICGNRRQC
G*SQGSVQTSRGLPR*RE*LRCRGGADYEAPET
HTGGSVGRQTQGDAEEAPTVCHARPEVQR*QCAS
PDFLLPHEPQHHDSKSQSDNCHGADHPHQCR*L
AVS*LSPELLVSWGSWKENSESSQSVSV**SWH
PDFERLCT*ARSPLFVGAEAGWPPLPQRAAPANS
DC*PLAERQPHGDHHETSEDQGAVPPGPPQTVC
IPRTWHCASYQ*LPVPLPCQGCLSPGEMGDSCP
*GLHGAALHQRHCGCGTGWGHQSLLHGAHRKGHS
QTAGRCGVEPWLLLHPTCFPALFELERGENQQQ
R*QHSGHGGRSQCVLQGAVWPLAQPPAVDQPAA
AAVCAAGCLPGDREP*RQCGGAQGISPGEDVSAA
LQGS*QDEAI*IQPSSGILQPSLISRADRCFPQ
GLTLSTGLLLSALAHM*LLIFSKDTSQLKSVT*
PVAFVCGSWQGGFAVWKGRWEL*HSVKKHL*RDF
FSAANKSRVEKDSNSAERCS

Sequence NM_001002878

GGGCAGTGGGGCTGGGAGATGGGGCGGGGCTGGGCGCTCCGGGACTGAGGTGCTGATCGCCCAAAGGGCGCCCCTGGGCTGGGGTCTGACCCGGCTTCTC
CTCCCTCACTACCCGCGGTCCCCGGCCTCTCGGCGCTGCGCTGTTACCTCATCCGAAAAGTGTTCGCTGCAGCATCCTCAGGCTTCACGTTGGAGCCTCT
TGATTGTGGTGTTCAGCATTATTTAAGATCACCTATGCATCATCTGAAGCCACAGTCCCAGGTCATCTTCATCACCTTCCCCAGTGGAATGCCTCCTGTG
TTAAAGCCCACAACAACCCCTGTGGAGGAACAACCATGTCATCAGAATCGAGCAAAAAACGGAAGCCCAAAGTGATCCGAAGCGATGGAGCCCCAGCTGA
AGGAAAGCGGAATCGATCTGACACCGAGCAGGAAGGTAAATACTACAGTGAGGAGGCCGAGGTGGATCTGCGGGACCCTGGCAGAGACTATGAGTTATAC
AAGTACACCTGCCAGGAGCTACAGAGGCTGATGGCTGAGATCCAAGACCTGAAGAGCAGGGGTGGCAAGGATGTGGCAATAGAAATAGAAGAACGGAGGA
TCCAGAGCTGTGTGCATTTCATGACTCTAAAGAAGCTTAACCGATTAGCCCACATCAGGTTGAAGAAAGGAAGAGATCAGACCCACGAGGCTAAGCAGAA
AGTAGATGCCTATCATCTGCAGCTCCAGAACCTGTTGTATGAGGTGATGCACCTACAGAAGGAGATCACCAAATGTTTGGAGTTTAAGTCAAAGCATGAA
GAAATTGATCTGGTCAGTTTAGAGGAGTTTTATAAGGAGGCTCCACCAGATATCAGCAAGGCCGAAGTCACCATGGGAGACCCTCACCAGCAAACACTGG
CACGTCTGGACTGGGAGCTGGAGCAGCGGAAAAGGCTGGCAGAGAAGTACCGAGAGTGCCTATCTAACAAGGAGAAGATTCTCAAGGAGATTGAGGTGAA
GAAGGAGTACCTGAGCAGCCTCCAGCCCCGCCTCAACAGCATCATGCAGGCTTCCCTTCCGGTGCAGGAGTACCTGTTTATGCCATTCGACCAGGCTCAC
AAGCAGTATGAGACAGCCAGACACCTGCCGCCTCCCCTCTATGTCCTCTTTGTTCAGGCCACTGCGTATGGGCAGGCCTGTGATAAGACGTTATCTGTGG
CAATCGAAGGCAGTGTGGATGAAGCCAAGGCTCTGTTCAAACCTCCAGAGGACTCCCAAGATGACGAGAGTGACTCAGATGCCGAGGAGGAGCAGACTAC
GAAGCGCCGGAGACCCACACTGGGGGTTCAGTTGGACGACAAACGCAAGGAGATGCTGAAGAGGCACCCACTGTCTGTCATGCTCGACCTGAAGTGCAAA
GATGACAGTGTGCTTCACCTGACTTTCTACTACCTCATGAACCTCAACATCATGACAGTAAAAGCCAAAGTGACAACTGCCATGGAGCTGATCACCCCCA
TCAGTGCAGGTGACTTGCTGTCTCCTGACTCAGTCCTGAGTTGCTTGTATCCTGGGGATCATGGAAAGAAAACTCCGAATCCAGCCAATCAGTATCAGTT
TGATAAAGTTGGCATCCTGACTTTGAGCGACTATGTACTTGAGCTAGGTCACCCCTATTTGTGGGTGCAGAAGCTGGGTGGCCTCCACTTCCCCAAAGAG
CAGCCCCAGCAAACAGTGATTGCTGACCACTCGCTGAGCGCCAGCCACATGGAGACCACCATGAAACTTCTGAAGACCAGGGTGCAGTCCCGCCTGGCCC
TCCACAAACAGTTTGCATCCCTAGAACATGGCATTGTGCCAGTTACCAGTGATTGCCAGTACCTCTTCCCTGCCAAGGTTGTCTCTCGCCTGGTGAAATG
GGTGACAGTTGCCCATGAGGATTACATGGAGCTGCACTTCACCAAAGACATTGTGGATGCGGGACTGGCTGGGGACACCAATCTCTACTACATGGCGCTC
ATCGAAAGGGGCACAGCCAAACTGCAGGCCGCTGTGGTGTTGAACCCTGGCTACTCCTCCATCCCACCTGTTTTCCAGCTCTGTTTGAACTGGAAAGGGG
AGAAAACCAACAGCAACGATGACAACATTCGGGCCATGGAGGGCGAAGTCAATGTGTGCTACAAGGAGCTGTGTGGCCCTTGGCCCAGCCACCAGCTGTT
GACCAACCAGCTGCAGCGGCTGTGTGTGCTGCTGGATGTTTACCTGGAGACCGAGAGCCATGACGACAGTGTGGAGGGGCCCAAGGAATTTCCCCAGGAG
AAGATGTGTCTGCGGCTCTTCAGGGGTCCTAGCAGGATGAAGCCATTTAAATACAACCATCCTCAGGGATTCTTCAGCCATCGCTGATCTCCCGCGCAGA
CCGTTGTTTCCCCCAAGGCCTCACCCTGAGCACTGGGCTTCTGCTTTCTGCTCTGGCCCACATGTGACTCTTGATATTCTCCAAAGACACCAGCCAATTA
AAAAGCGTCACCTGACCAGTGGCCTTTGTCTGTGGTTCCTGGCAAGGTGGCTTTGCAGTCTGGAAGGGCAGGTGGGAGCTGTGACACAGTGTGAAAAAGC
ATTTGTAGAGAGACTTTTTCTCAGCAGCCAATAAAAGCAGAGTGGAAAAAGATTCCAATTCTGCAGAGAGATGCTCA
  • refseq_THOC5.F4 (forward primer)
  • refseq_THOC5.R3 (reverse primer)
  • Single exon skipping

Frame 0

GQWGWEMGRGWALRD*GADRPKGAPGLGSDPAS
PPSLPAVPGLSALRCYLIRKVFAAASSGFTLEPL
DCGVQHYLRSPMHHLKPQSQVIFITFPSGMPPV
LKPTTTPVEEQPCHQNRAKNGSPK*SEAMEPQL
KESGIDLTPSRKVNTTVRRPRWICGTLAETMSYT
STPARSYRG*WLRSKT*RAGVARMWQ*K*KNGG
SRAVCIS*L*RSLTD*PTSG*RKEEIRPTRLSR
K*MPIICSSRTCCMR*CTYRRRSPNVWSLSQSMK
KLIWSV*RSFIRRLHQISARPKSPWETLTSKHW
HVWTGSWSSGKGWQRSTESAYLTRRRFSRRLR*
RRST*AASSPASTASCRLPFRCRSTCLCHSTRLT
SSMRQPDTCRLPSMSSLFRPLRMGRPVIRRYLW
QSKAVWMKPRLCSNLQRTPKMTRVTQMPRRSRL
RSAGDPHWGFSWTTNARRC*RGTHCLSCST*SAK
MTVCFT*LSTTS*TSTS*Q*KPK*QLPWS*SPP
SVQVTCCLLTQS*VACILGIMERKLRIQPISIS
LIKLAS*L*ATMYLS*VTPICGCRSWVASTSPKS
SPSKQ*LLTTR*APATWRPP*NF*RPGCSPAWP
STNSLHP*NMALCQLPVIASTSSLPRLSLAW*N
G*QLPMRITWSCTSPKTLWMRDWLGTPISTTWRS
SKGAQPNCRPLWC*TLATPPSHLFSSSV*TGKG
RKPTATMTTFGPWRAKSMCATRSCVALGPATSC
*PTSCSGCVCCWMFTWRPRAMTTVWRGPRNFPRR
RCVCGSSGVLAG*SHLNTTILRDSSAIADLPRR
PLFPPRPHPEHWASAFCSGPHVTLDILQRHQPI
KKRHLTSGLCLWFLARWLCSLEGQVGAVTQCEKA
FVERLFLSSQ*KQSGKRFQFCREML

Frame 1

GSGAGRWGGAGRSGTEVLIAQRAPLGWGLTRLL
LPHYPRSPASRRCAVTSSEKCSLQHPQASRWSL
LIVVFSII*DHLCII*SHSPRSSSSPSPVECLLC
*SPQQPLWRNNHVIRIEQKTEAQSDPKRWSPS*
RKAESI*HRAGR*ILQ*GGRGGSAGPWQRL*VI
QVHLPGATEADG*DPRPEEQGWQGCGNRNRRTED
PELCAFHDSKEA*PISPHQVEERKRSDPRG*AE
SRCLSSAAPEPVV*GDAPTEGDHQMFGV*VKA*
RN*SGQFRGVL*GGSTRYQQGRSHHGRPSPANTG
TSGLGAGAAEKAGREVPRVPI*QGEDSQGD*GE
EGVPEQPPAPPQQHHAGFPSGAGVPVYAIRPGS
QAV*DSQTPAASPLCPLCSGHCVWAGL**DVICG
NRRQCG*SQGSVQTSRGLPR*RE*LRCRGGADY
EAPETHTGGSVGRQTQGDAEEAPTVCHARPEVQ
R*QCASPDFLLPHEPQHHDSKSQSDNCHGADHPH
QCR*LAVS*LSPELLVSWGSWKENSESSQSVSV
**SWHPDFERLCT*ARSPLFVGAEAGWPPLPQR
AAPANSDC*PLAERQPHGDHHETSEDQGAVPPGP
PQTVCIPRTWHCASYQ*LPVPLPCQGCLSPGEM
GDSCP*GLHGAALHQRHCGCGTGWGHQSLLHGA
HRKGHSQTAGRCGVEPWLLLHPTCFPALFELERG
ENQQQR*QHSGHGGRSQCVLQGAVWPLAQPPAV
DQPAAAAVCAAGCLPGDREP*RQCGGAQGISPG
EDVSAALQGS*QDEAI*IQPSSGILQPSLISRAD
RCFPQGLTLSTGLLLSALAHM*LLIFSKDTSQL
KSVT*PVAFVCGSWQGGFAVWKGRWEL*HSVKK
HL*RDFFSAANKSRVEKDSNSAERCS

Frame 2

AVGLGDGAGLGAPGLRC*SPKGRPWAGV*PGFS
SLTTRGPRPLGAALLPHPKSVRCSILRLHVGAS
*LWCSALFKITYASSEATVPGHLHHLPQWNASC
VKAHNNPCGGTTMSSESSKKRKPKVIRSDGAPAE
GKRNRSDTEQEGKYYSEEAEVDLRDPGRDYELY
KYTCQELQRLMAEIQDLKSRGGKDVAIEIEERR
IQSCVHFMTLKKLNRLAHIRLKKGRDQTHEAKQK
VDAYHLQLQNLLYEVMHLQKEITKCLEFKSKHE
EIDLVSLEEFYKEAPPDISKAEVTMGDPHQQTL
ARLDWELEQRKRLAEKYRECLSNKEKILKEIEVK
KEYLSSLQPRLNSIMQASLPVQEYLFMPFDQAH
KQYETARHLPPPLYVLFVQATAYGQACDKTLSV
AIEGSVDEAKALFKPPEDSQDDESDSDAEEEQTT
KRRRPTLGVQLDDKRKEMLKRHPLSVMLDLKCK
DDSVLHLTFYYLMNLNIMTVKAKVTTAMELITP
ISAGDLLSPDSVLSCLYPGDHGKKTPNPANQYQF
DKVGILTLSDYVLELGHPYLWVQKLGGLHFPKE
QPQQTVIADHSLSASHMETTMKLLKTRVQSRLA
LHKQFASLEHGIVPVTSDCQYLFPAKVVSRLVKW
VTVAHEDYMELHFTKDIVDAGLAGDTNLYYMAL
IERGTAKLQAAVVLNPGYSSIPPVFQLCLNWKG
EKTNSNDDNIRAMEGEVNVCYKELCGPWPSHQLL
TNQLQRLCVLLDVYLETESHDDSVEGPKEFPQE
KMCLRLFRGPSRMKPFKYNHPQGFFSHR*SPAQ
TVVSPKASP*ALGFCFLLWPTCDS*YSPKTPAN*
KASPDQWPLSVVPGKVALQSGRAGGSCDTV*KS
ICRETFSQQPIKAEWKKIPILQRDA

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