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Sequences

Sequence NM_172226

GCTGAGCCGAGCCGAGCCGAGCTGGGGGCGCAGAGCGCGGGAGGCGGCGGCGGCGCGGAGCCCAGGTGGCTCCGCTGCCGGATGGGAGTGCCCCAGTGTG CTGGATGAAGCTGGCGCATGCACCATGTCATCATGTGTCTCTAGCCAGCCCAGCAGCAACCGGGCCGCCCCCCAGGATGAGCTGGGGGGCAGGGGCAGCA GCAGCAGCGAAAGCCAGAAGCCCTGTGAGGCCCTGCGGGGCCTCTCATCCTTGAGCATCCACCTGGGCATGGAGTCCTTCATTGTGGTCACCGAGTGTGA GCCGGGCTGTGCTGTGGACCTCGGCTTGGCGCGGGACCGGCCCCTGGAGGCCGATGGCCAAGAGGTCCCCCTTGACACCTCCGGGTCCCAGGCCCGGCCC CACCTCTCCGGTCGCAAGCTGTCTCTGCAAGAGCGGTCCCAGGGTGGGCTGGCAGCCGGTGGCAGCCTGGACATGAACGGACGCTGCATCTGCCCGTCCC TGCCCTACTCACCCGTCAGCTCCCCGCAGTCCTCGCCTCGGCTGCCCCGGCGGCCGACAGTGGAGTCTCACCACGTCTCCATCACGGGTATGCAGGACTG TGTGCAGCTGAATCAGTATACCCTGAAGGATGAAATTGGAAAGGGCTCCTATGGTGTCGTCAAGTTGGCCTACAATGAAAATGACAATACCTACTATGCA ATGAAGGTGCTGTCCAAAAAGAAGCTGATCCGGCAGGCCGGCTTTCCACGTCGCCCTCCACCCCGAGGCACCCGGCCAGCTCCTGGAGGCTGCATCCAGC CCAGGGGCCCCATTGAGCAGGTGTACCAGGAAATTGCCATCCTCAAGAAGCTGGACCACCCCAATGTGGTGAAGCTGGTGGAGGTCCTGGATGACCCCAA TGAGGACCATCTGTACATGGTGTTCGAACTGGTCAACCAAGGGCCCGTGATGGAAGTGCCCACCCTCAAACCACTCTCTGAAGACCAGGCCCGTTTCTAC TTCCAGGATCTGATCAAAGGCATCGAGTACTTACACTACCAGAAGATCATCCACCGTGACATCAAACCTTCCAACCTCCTGGTCGGAGAAGATGGGCACA TCAAGATCGCTGACTTTGGTGTGAGCAATGAATTCAAGGGCAGTGACGCGCTCCTCTCCAACACCGTGGGCACGCCCGCCTTCATGGCACCCGAGTCGCT CTCTGAGACCCGCAAGATCTTCTCTGGGAAGGCCTTGGATGTTTGGGCCATGGGTGTGACACTATACTGCTTTGTCTTTGGCCAGTGCCCATTCATGGAC GAGCGGATCATGTGTTTACACAGTAAGATCAAGAGTCAGGCCCTGGAATTTCCAGACCAGCCCGACATAGCTGAGGACTTGAAGGACCTGATCACCCGTA TGCTGGACAAGAACCCCGAGTCGAGGATCGTGGTGCCGGAAATCAAGCTGCACCCCTGGGTCACGAGGCATGGGGCGGAGCCGTTGCCGTCGGAGGATGA GAACTGCACGCTGGTCGAAGTGACTGAAGAGGAGGTCGAGAACTCAGTCAAACACATTCCCAGCTTGGCAACCGTGATCCTGGTGAAGACCATGATACGT AAACGCTCCTTTGGGAACCCATTCGAGGGCAGCCGGCGGGAGGAACGCTCACTGTCAGCGCCTGGAAACTTGCTCACGAAGCAAGGCAGCGAAGACAACC TCCAGGGCACCGACCCGCCCCCCGTGGGGGAGGAGGAAGTGCTCTTGTGAGAGGCAGTCCCTGCGTGGAAAGTTGCTGGGCCCCCGCCCCCGGCTCCCCC GCACGCATGCATCCACTGCGGCCGGAGGAGGCCATGGAGCCCGAGTAGCTGCCTGGATCGCTCGACCTCGCATGCGCGCCGCGTCGCCTCTGGGGGGCTG CTGCACCGCGTTTCCATAGCAGCATGTCCTACGGAAACCCAGCACGTGTGTAGAGCCTCGATCGTCATCTCTGGTTATTTGTTTTTTCCTTTGTTGTTTT AAAGGGGACAAAAAAAAAAAAAGGACTTGACTCCATGACGTCGACCGTGGCCGCTGGCTGGCTGGACAGGCGGGTGTGAGGAGTTGCAGACCCAAACCCA CGTGCATTTTGGGACAATTGCTTTTTAAAACGTTTTTATGCCAAAAATCCTTCATTGTGATTTTCAGAACCACGTCAGATATACCAAGTGACTGTGTGTG GGGTTTGACAACTGTGGAAAGGCGAGCAGAAAACTCCGGCGGTCTGAGGCCATGGAGGTGGTTGCTGCATTTGAGAGGGAGTAGGGGGCTAGATGTGGCT CCTAGTGCAAACCGGAAACCATGGCACCTTCCAGAGCCGTGGTCTCAAGGAGTCAGAGCAGGGCTGGCCCTCAGTAGCTGCAGGGAGCTTTGATGCAACT TATTTGTAAGAAGGATTTTTAAATTTTTTATGGGTAGAATTGTAGTCAGGAAAACAGAAAGGGCTTGAAATTTAATAAGTGCTGCTGGAAGGGGATTTTC CAAGCCTGGAAGGGTATTCAGCAGCTGTGGTGGGGAAACATTTCTCCTGAAAGACTGAACGTGTTTCTTCATGACAGCTGCTCAAAGCAGGTTTCTGAGA TAGCTGACCGAGCTCTGGTAAATCTCTTTGTCAAATTACGAAAACTTCAGGGTGAAATCCTATGCTTCCATGTACATTACATGGCTTAAGATTAAACAAA AACATTTTTCAAGTCTCTAACTAGAGTGAACTCTAGAGCACAGTAGTTCAGAAACTATTTAGAGCTTCCAGGATATATTTCACAGCTTCAGGCATGTGAT CAGTTAGAGCCGATGAAACCTATGCCCGCCTGTATATATATTAGCAGCTTAGCTAGTTCATAACCTGTATATTCTAAAGACTGCTAAGGTTTTGTTTTCA TTTTAAATCCTAGCTGATTGTTGTGGTCAATGAAATACCCAGTTTCTGGAGGGCCAGGTGGGAAATGCTTTCACTGGACCAACACACAAATGATCATCCT GAGGATCTGAGCTTCCCTAGACTCCACACAATAACCTTGGGGCACCCTTTTAGAGAAGACTGTTGAAACCCACAGCACTCGTTGGGGTATGAGGAAACCA GGGCTTGGCACAGGAAGTTCCCCTTTGTAGCTAAAAGTCCAGAAAGAAAGGGTTCATCTTTTTGACTTCCAACTGATATTGGGAAGTTTGGTTGAGGTTC AAGTGTGACTCCTTCCAGAGCCACAGGTAGGGGAGTGTGAAGTTGAGGGGGAGGAAAGCTGGAAGGACTCTGCCTTGGGAGATTCCCAGCTCTGCTTTCC AGCGCTTGGTGGAATCTGGGCTGGGGAAAGACGGCACCGGGAAACTCTGCTTCCCCATTGTTTCCATCTGATCAGCTGTGGTGTGAGGACTTCTCAGACA AAGGCAAGGCCTCGTGCCCCTGCCCAGCCCATTCATGGAGCCCTGGGCCTTCTTGGCTTCCATAGATCCTAAGCTCTTGACTGTAGTTTAGCCAGACTTG TTTTGCTATCTTATAAGCAGTTCAGAATTAGGGAATGCTGGTTTTGAAGAGCAAAGGACAGGTAGTCTAGAGAGGGTCGTCTGGCCTGCTTGCTGGGTCT TTGTAACCCAGCACTTCCTCTTGCCCTCCTGGCTTTATGTTTATGGGGAGAGGACTCAATAGCTCCACCCCTTCTGGCACCAGATGGGGCTTGGTTAGTT TGCAATAAGCACCTTGCAGAGGTTAAAGCCAGCGGGTCCCTAGTCTTAGGCCCAGCCTGCTTGTGTGGGCTCTGGCCTGGCCTGGTGGCTGGCCCAGGGG GCAGCAGTGCTTAGAGCTTCTGCAGGGCTTCTCTTGTTTACACAGCTGCATCAGACAATGCCATTTCTCCCCACCACGGAACCTTCCATCTAAGATTTCT TCCAGGGAATGCCAGCAATCAGGCAGCACCCAGCTGTGGGGGCAGTGGGGTGGGGGAGACCCACATTGATGACTTTTTTTTTTTCTTTTAATGAAGAAAC ACCAAAGAAAGCTGTGGAAAGGACCTGCCCCACATGAAAAGGATAAGCCAAGATGGCTGTAAACACAGAGCATTTGAGCTGCCACTCTTGGAGCACATTG ATTTTTCAAAAGCCAGCTCTGTCAGGAAAGGAGGTGCTGTTATGAGCAGCTCTTCCAGTGGGCAAAGAGGACGCCCATAATTTCTTCCATTGCTAGCTCA TCTGTGGGACCAATTTGGTGTAAGCAACCTGTGGCCTGCACTTGTGGCCTCGAAGGAAGCACAAACCCTCCATCCACTTCCCATTTCCTCTGCCCTTTTC CACCTCCCCCTTCCATCCCACCAGCTGCCAGTGGCTCCCAGAAAGCCTTATTGAGCCCCTTGTTGACACTTGGGGCTGCGGAGGCCTCTCCCTACTGGTC TGGCCTTTCCTGAGAGGCAGGTCTTCCGTCCTCAGAGCCTTTCTGGAACAAGGAGAATGCCTGTGCAGGTGGACACACAGGCCTGGCCTGTCGCTCTCAC TTGTCTTCCAGCGGGGAGCTTCACGTTGCCGAGTGGAAGAACCATGACCTCCACTTGCTTCCAAGGTGCTAGGGAAGTTTCAGGGTACGCTGGTTCCCCT CTCCAGCTGGAGGCCGAGTTTCTGGGGACTGCAGATTTTTCTACTCTGTGATCGATTCAATGCCCGATGCTTCTGTTTCATTCCCGACCCTTTCTACTAT GCATTTTCCTTTTATCAGGTGTATAAAGTTAAATACTGTGTATTTATCACTAAAAAGTACATGAACTTAAGAGACAACTAAGCCTTTCGTGTTTTTCCAC AGGTGTTTAAGCTTCTCTGTACAGTTGAAATAAACAGACAGCAAAATGGTGCCAA

refseq_CAMKK2.F2 (forward primer)
refseq_CAMKK2.R2 (reverse primer)
Single exon skipping

Frame 0

AEPSRAELGAQSAGGGGGAEPRWLRCRMGVPQC AG*SWRMHHVIMCL*PAQQQPGRPPG*AGGQGQQ QQRKPEAL*GPAGPLILEHPPGHGVLHCGHRV* AGLCCGPRLGAGPAPGGRWPRGPP*HLRVPGPA PPLRSQAVSARAVPGWAGSRWQPGHERTLHLPVP ALLTRQLPAVLASAAPAADSGVSPRLHHGYAGL CAAESVYPEG*NWKGLLWCRQVGLQ*K*QYLLC NEGAVQKEADPAGRLSTSPSTPRHPASSWRLHPA QGPH*AGVPGNCHPQEAGPPQCGEAGGGPG*PQ *GPSVHGVRTGQPRARDGSAHPQTTL*RPGPFL LPGSDQRHRVLTLPEDHPP*HQTFQPPGRRRWAH QDR*LWCEQ*IQGQ*RAPLQHRGHARLHGTRVA L*DPQDLLWEGLGCLGHGCDTILLCLWPVPIHG RADHVFTQ*DQESGPGISRPARHS*GLEGPDHPY AGQEPRVEDRGAGNQAAPLGHEAWGGAVAVGG* ELHAGRSD*RGGRELSQTHSQLGNRDPGEDHDT *TLLWEPIRGQPAGGTLTVSAWKLAHEARQRRQP PGHRPAPRGGGGSALVRGSPCVESCWAPAPGSP ARMHPLRPEEAMEPE*LPGSLDLACAPRRLWGA AAPRFHSSMSYGNPARV*SLDRHLWLFVFSFVVL KGTKKKKGLDSMTSTVAAGWLDRRV*GVADPNP RAFWDNCFLKRFYAKNPSL*FSEPRQIYQVTVC GV*QLWKGEQKTPAV*GHGGGCCI*EGVGG*MWL LVQTGNHGTFQSRGLKESEQGWPSVAAGSFDAT YL*EGFLNFLWVEL*SGKQKGLEI**VLLEGDF PSLEGYSAAVVGKHFS*KTERVSS*QLLKAGF*D S*PSSGKSLCQITKTSG*NPMLPCTLHGLRLNK NIFQVSN*SEL*STVVQKLFRASRIYFTASGM* SVRADETYARLYIY*QLS*FITCIF*RLLRFCFH FKS*LIVVVNEIPSFWRARWEMLSLDQHTNDHP EDLSFPRLHTITLGHPFREDC*NPQHSLGYEET RAWHRKFPFVAKSPERKGSSF*LPTDIGKFG*GS SVTPSRATGRGV*S*GGGKLEGLCLGRFPALLS SAWWNLGWGKTAPGNSASPLFPSDQLWCEDFSD KGKASCPCPAHSWSPGPSWLP*ILSS*L*FSQTC FAIL*AVQN*GMLVLKSKGQVV*RGSSGLLAGS L*PSTSSCPPGFMFMGRGLNSSTPSGTRWGLVS LQ*APCRG*SQRVPSLRPSLLVWALAWPGGWPRG QQCLELLQGFSCLHSCIRQCHFSPPRNLPSKIS SRECQQSGSTQLWGQWGGGDPH**LFFFLLMKK HQRKLWKGPAPHEKDKPRWL*TQSI*AATLGAH* FFKSQLCQERRCCYEQLFQWAKRTPIISSIASS SVGPIWCKQPVACTCGLEGSTNPPSTSHFLCPF PPPPSIPPAASGSQKALLSPLLTLGAAEASPYWS GLS*EAGLPSSEPFWNKENACAGGHTGLACRSH LSSSGELHVAEWKNHDLHLLPRC*GSFRVRWFP SPAGGRVSGDCRFFYSVIDSMPDASVSFPTLSTM HFPFIRCIKLNTVYLSLKST*T*ETTKPFVFFH RCLSFSVQLK*TDSKMVP

Frame 1

LSRAEPSWGRRAREAAAARSPGGSAAGWECPSV LDEAGACTMSSCVSSQPSSNRAAPQDELGGRGS SSSESQKPCEALRGLSSLSIHLGMESFIVVTECE PGCAVDLGLARDRPLEADGQEVPLDTSGSQARP HLSGRKLSLQERSQGGLAAGGSLDMNGRCICPS LPYSPVSSPQSSPRLPRRPTVESHHVSITGMQDC VQLNQYTLKDEIGKGSYGVVKLAYNENDNTYYA MKVLSKKKLIRQAGFPRRPPPRGTRPAPGGCIQ PRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPN EDHLYMVFELVNQGPVMEVPTLKPLSEDQARFY FQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDGH IKIADFGVSNEFKGSDALLSNTVGTPAFMAPESL SETRKIFSGKALDVWAMGVTLYCFVFGQCPFMD ERIMCLHSKIKSQALEFPDQPDIAEDLKDLITR MLDKNPESRIVVPEIKLHPWVTRHGAEPLPSEDE NCTLVEVTEEEVENSVKHIPSLATVILVKTMIR KRSFGNPFEGSRREERSLSAPGNLLTKQGSEDN LQGTDPPPVGEEEVLL*EAVPAWKVAGPPPPAPP HACIHCGRRRPWSPSSCLDRSTSHARRVASGGL LHRVSIAACPTETQHVCRASIVISGYLFFPLLF *RGQKKKKDLTP*RRPWPLAGWTGGCEELQTQTH VHFGTIAF*NVFMPKILHCDFQNHVRYTK*LCV GFDNCGKASRKLRRSEAMEVVAAFERE*GARCG S*CKPETMAPSRAVVSRSQSRAGPQ*LQGALMQL ICKKDF*IFYG*NCSQENRKGLKFNKCCWKGIF QAWKGIQQLWWGNISPERLNVFLHDSCSKQVSE IADRALVNLFVKLRKLQGEILCFHVHYMA*D*TK TFFKSLTRVNSRAQ*FRNYLELPGYISQLQACD QLEPMKPMPACIYISSLASS*PVYSKDC*GFVF ILNPS*LLWSMKYPVSGGPGGKCFHWTNTQMIIL RI*ASLDSTQ*PWGTLLEKTVETHSTRWGMRKP GLGTGSSPL*LKVQKERVHLFDFQLILGSLVEV QV*LLPEPQVGECEVEGEESWKDSALGDSQLCFP ALGGIWAGERRHRETLLPHCFHLISCGVRTSQT KARPRAPAQPIHGALGLLGFHRS*ALDCSLARL VLLSYKQFRIRECWF*RAKDR*SREGRLACLLGL CNPALPLALLALCLWGEDSIAPPLLAPDGAWLV CNKHLAEVKASGSLVLGPACLCGLWPGLVAGPG GSSA*SFCRASLVYTAASDNAISPHHGTFHLRFL PGNASNQAAPSCGGSGVGETHIDDFFFFF**RN TKESCGKDLPHMKRISQDGCKHRAFELPLLEHI DFSKASSVRKGGAVMSSSSSGQRGRP*FLPLLAH LWDQFGVSNLWPALVASKEAQTLHPLPISSALF HLPLPSHQLPVAPRKPY*APC*HLGLRRPLPTG LAFPERQVFRPQSLSGTRRMPVQVDTQAWPVALT CLPAGSFTLPSGRTMTSTCFQGAREVSGYAGSP LQLEAEFLGTADFSTL*SIQCPMLLFHSRPFLL CIFLLSGV*S*ILCIYH*KVHELKRQLSLSCFST GV*ASLYS*NKQTAKWCQ

Frame 2

*AEPSRAGGAERGRRRRRGAQVAPLPDGSAPVC WMKLAHAPCHHVSLASPAATGPPPRMSWGAGAA AAAKARSPVRPCGASHP*ASTWAWSPSLWSPSV SRAVLWTSAWRGTGPWRPMAKRSPLTPPGPRPGP TSPVASCLCKSGPRVGWQPVAAWT*TDAASARP CPTHPSAPRSPRLGCPGGRQWSLTTSPSRVCRT VCS*ISIP*RMKLERAPMVSSSWPTMKMTIPTMQ *RCCPKRS*SGRPAFHVALHPEAPGQLLEAASS PGAPLSRCTRKLPSSRSWTTPMW*SWWRSWMTP MRTICTWCSNWSTKGP*WKCPPSNHSLKTRPVST SRI*SKASSTYTTRRSSTVTSNLPTSWSEKMGT SRSLTLV*AMNSRAVTRSSPTPWARPPSWHPSR SLRPARSSLGRPWMFGPWV*HYTALSLASAHSWT SGSCVYTVRSRVRPWNFQTSPT*LRT*RT*SPV CWTRTPSRGSWCRKSSCTPGSRGMGRSRCRRRM RTARWSK*LKRRSRTQSNTFPAWQP*SW*RP*YV NAPLGTHSRAAGGRNAHCQRLETCSRSKAAKTT SRAPTRPPWGRRKCSCERQSLRGKLLGPRPRLP RTHASTAAGGGHGARVAAWIARPRMRAASPLGGC CTAFP*QHVLRKPSTCVEPRSSSLVICFFLCCF KGDKKKKRT*LHDVDRGRWLAGQAGVRSCRPKP TCILGQLLFKTFLCQKSFIVIFRTTSDIPSDCVW GLTTVERRAENSGGLRPWRWLLHLRGSRGLDVA PSANRKPWHLPEPWSQGVRAGLALSSCREL*CN LFVRRIFKFFMGRIVVRKTERA*NLISAAGRGFS KPGRVFSSCGGETFLLKD*TCFFMTAAQSRFLR *LTELW*ISLSNYENFRVKSYASMYITWLKIKQ KHFSSL*LE*TLEHSSSETI*SFQDIFHSFRHVI S*SR*NLCPPVYILAA*LVHNLYILKTAKVLFS F*ILADCCGQ*NTQFLEGQVGNAFTGPTHK*SS *GSELP*TPHNNLGAPF*RRLLKPTALVGV*GNQ GLAQEVPLCS*KSRKKGFIFLTSN*YWEVWLRF KCDSFQSHR*GSVKLRGRKAGRTLPWEIPSSAF QRLVESGLGKDGTGKLCFPIVSI*SAVV*GLLRQ RQGLVPLPSPFMEPWAFLASIDPKLLTVV*PDL FCYLISSSELGNAGFEEQRTGSLERVVWPACWV FVTQHFLLPSWLYVYGERTQ*LHPFWHQMGLG*F AISTLQRLKPAGP*S*AQPACVGSGLAWWLAQG AAVLRASAGLLLFTQLHQTMPFLPTTEPSI*DF FQGMPAIRQHPAVGAVGWGRPTLMTFFFSFNEET PKKAVERTCPT*KG*AKMAVNTEHLSCHSWSTL IFQKPALSGKEVLL*AALPVGKEDAHNFFHC*L ICGTNLV*ATCGLHLWPRRKHKPSIHFPFPLPFS TSPFHPTSCQWLPESLIEPLVDTWGCGGLSLLV WPFLRGRSSVLRAFLEQGECLCRWTHRPGLSLS LVFQRGASRCRVEEP*PPLASKVLGKFQGTLVPL SSWRPSFWGLQIFLLCDRFNARCFCFIPDPFYY AFSFYQVYKVKYCVFITKKYMNLRDN*AFRVFP QVFKLLCTVEINRQQNGA

Sequence NM_153499

GAGCCTGGGGAGGTCGAGGGTGCAGCGAGCCGTGATCGTGCTACTGCACTCCAGCCTGGGCAACACAGAGAGACCCTGTCTCAAAACAAACAAACAAACA AACAAACAAACAAACAAAAAAAACAAAGAAAAAAAAATGGGAGTGGGCCGGGCGCGGTGACTCACACCTGTAATCCCAGCACTTTCGGAGGCCAAGGCGG GTGGATCACGAGGTCAGGAATTCAAGATTAGCCTGGACAACATGGTGAAACCCCATCTCTACGAAAAATACAAAAATTAGCCAAGTATGGTGGCCGGCGC CTGTAATCCCAGCTACTCGGGAGACTGAGGCAGAGAACTGCTTGAACCTGGGAGGCAGAGGTTGCAGTGATCCGAGATCGCGTCACTGCACTCCAGCGTG GGCGACAGAGCGAGACTCCGTTTCAGAAAAGAAAAAAAAAAAAAAAAAAAAGGGAGTCGGGGTGGAGCTCTCATTGGCTCGTTGCATGTGAGTGTCCCTA CGGCCTAGAAATACAAGAGAAGCACATCGGAACGGGCTGGAAATCCACCCAGTTAACTAGAGGGCTTTGAACCTTTTATTAACTTGGAGGTTGACTCTCC TGTCAACTCGATTCCCTTTTGGCTGTTTGGCAGGGTCAGTGAGACATCCCCTGGGTCGCTCGACCCCGTAGGACGGTTCAGGGAGCCCTCCAGGTCTTCG TTTCTCCTCTTCCCCGCACAGTGCTGTTATCCAGCTGGGGGATCCAACGCACACTTAAGGCTCCAGCAAAGTGGCTCCGCTGCCGGATGGGAGTGCCCCA GTGTGCTGGATGAAGCTGGCGCATGCACCATGTCATCATGTGTCTCTAGCCAGCCCAGCAGCAACCGGGCCGCCCCCCAGGATGAGCTGGGGGGCAGGGG CAGCAGCAGCAGCGAAAGCCAGAAGCCCTGTGAGGCCCTGCGGGGCCTCTCATCCTTGAGCATCCACCTGGGCATGGAGTCCTTCATTGTGGTCACCGAG TGTGAGCCGGGCTGTGCTGTGGACCTCGGCTTGGCGCGGGACCGGCCCCTGGAGGCCGATGGCCAAGAGGTCCCCCTTGACACCTCCGGGTCCCAGGCCC GGCCCCACCTCTCCGGTCGCAAGCTGTCTCTGCAAGAGCGGTCCCAGGGTGGGCTGGCAGCCGGTGGCAGCCTGGACATGAACGGACGCTGCATCTGCCC GTCCCTGCCCTACTCACCCGTCAGCTCCCCGCAGTCCTCGCCTCGGCTGCCCCGGCGGCCGACAGTGGAGTCTCACCACGTCTCCATCACGGGTATGCAG GACTGTGTGCAGCTGAATCAGTATACCCTGAAGGATGAAATTGGAAAGGGCTCCTATGGTGTCGTCAAGTTGGCCTACAATGAAAATGACAATACCTACT ATGCAATGAAGGTGCTGTCCAAAAAGAAGCTGATCCGGCAGGCCGGCTTTCCACGTCGCCCTCCACCCCGAGGCACCCGGCCAGCTCCTGGAGGCTGCAT CCAGCCCAGGGGCCCCATTGAGCAGGTGTACCAGGAAATTGCCATCCTCAAGAAGCTGGACCACCCCAATGTGGTGAAGCTGGTGGAGGTCCTGGATGAC CCCAATGAGGACCATCTGTACATGGTGTTCGAACTGGTCAACCAAGGGCCCGTGATGGAAGTGCCCACCCTCAAACCACTCTCTGAAGACCAGGCCCGTT TCTACTTCCAGGATCTGATCAAAGGCATCGAGTACTTACACTACCAGAAGATCATCCACCGTGACATCAAACCTTCCAACCTCCTGGTCGGAGAAGATGG GCACATCAAGATCGCTGACTTTGGTGTGAGCAATGAATTCAAGGGCAGTGACGCGCTCCTCTCCAACACCGTGGGCACGCCCGCCTTCATGGCACCCGAG TCGCTCTCTGAGACCCGCAAGATCTTCTCTGGGAAGGCCTTGGATGTTTGGGCCATGGGTGTGACACTATACTGCTTTGTCTTTGGCCAGTGCCCATTCA TGGACGAGCGGATCATGTGTTTACACAGTAAGATCAAGAGTCAGGCCCTGGAATTTCCAGACCAGCCCGACATAGCTGAGGACTTGAAGGACCTGATCAC CCGTATGCTGGACAAGAACCCCGAGTCGAGGATCGTGGTGCCGGAAATCAAGCTGCACCCCTGGGTCACGAGGCATGGGGCGGAGCCGTTGCCGTCGGAG GATGAGAACTGCACGCTGGTCGAAGTGACTGAAGAGGAGGTCGAGAACTCAGTCAAACACATTCCCAGCTTGGCAACCGTGATCCTGGTGAAGACCATGA TACGTAAACGCTCCTTTGGGAACCCATTCGAGGGCAGCCGGCGGGAGGAACGCTCACTGTCAGCGCCTGGAAACTTGCTCACGAAGCAAGGCAGCGAAGA CAACCTCCAGGGCACCGACCCGCCCCCCGTGGGGGAGGAGGAAGTGCTCTTGTGAGAGGCAGTCCCTGCGTGGAAAGTTGCTGGGCCCCCGCCCCCGGCT CCCCCGCACGCATGCATCCACTGCGGCCGGAGGAGGCCATGGAGCCCGAGTAGCTGCCTGGATCGCTCGACCTCGCATGCGCGCCGCGTCGCCTCTGGGG GGCTGCTGCACCGCGTTTCCATAGCAGCATGTCCTACGGAAACCCAGCACGTGTGTAGAGCCTCGATCGTCATCTCTGGTTATTTGTTTTTTCCTTTGTT GTTTTAAAGGGGACAAAAAAAAAAAAAGGACTTGACTCCATGACGTCGACCGTGGCCGCTGGCTGGCTGGACAGGCGGGTGTGAGGAGTTGCAGACCCAA ACCCACGTGCATTTTGGGACAATTGCTTTTTAAAACGTTTTTATGCCAAAAATCCTTCATTGTGATTTTCAGAACCACGTCAGATATACCAAGTGACTGT GTGTGGGGTTTGACAACTGTGGAAAGGCGAGCAGAAAACTCCGGCGGTCTGAGGCCATGGAGGTGGTTGCTGCATTTGAGAGGGAGTAGGGGGCTAGATG TGGCTCCTAGTGCAAACCGGAAACCATGGCACCTTCCAGAGCCGTGGTCTCAAGGAGTCAGAGCAGGGCTGGCCCTCAGTAGCTGCAGGGAGCTTTGATG CAACTTATTTGTAAGAAGGATTTTTAAATTTTTTATGGGTAGAATTGTAGTCAGGAAAACAGAAAGGGCTTGAAATTTAATAAGTGCTGCTGGAAGGGGA TTTTCCAAGCCTGGAAGGGTATTCAGCAGCTGTGGTGGGGAAACATTTCTCCTGAAAGACTGAACGTGTTTCTTCATGACAGCTGCTCAAAGCAGGTTTC TGAGATAGCTGACCGAGCTCTGGTAAATCTCTTTGTCAAATTACGAAAACTTCAGGGTGAAATCCTATGCTTCCATGTACATTACATGGCTTAAGATTAA ACAAAAACATTTTTCAAGTCTCTAACTAGAGTGAACTCTAGAGCACAGTAGTTCAGAAACTATTTAGAGCTTCCAGGATATATTTCACAGCTTCAGGCAT GTGATCAGTTAGAGCCGATGAAACCTATGCCCGCCTGTATATATATTAGCAGCTTAGCTAGTTCATAACCTGTATATTCTAAAGACTGCTAAGGTTTTGT TTTCATTTTAAATCCTAGCTGATTGTTGTGGTCAATGAAATACCCAGTTTCTGGAGGGCCAGGTGGGAAATGCTTTCACTGGACCAACACACAAATGATC ATCCTGAGGATCTGAGCTTCCCTAGACTCCACACAATAACCTTGGGGCACCCTTTTAGAGAAGACTGTTGAAACCCACAGCACTCGTTGGGGTATGAGGA AACCAGGGCTTGGCACAGGAAGTTCCCCTTTGTAGCTAAAAGTCCAGAAAGAAAGGGTTCATCTTTTTGACTTCCAACTGATATTGGGAAGTTTGGTTGA GGTTCAAGTGTGACTCCTTCCAGAGCCACAGGTAGGGGAGTGTGAAGTTGAGGGGGAGGAAAGCTGGAAGGACTCTGCCTTGGGAGATTCCCAGCTCTGC TTTCCAGCGCTTGGTGGAATCTGGGCTGGGGAAAGACGGCACCGGGAAACTCTGCTTCCCCATTGTTTCCATCTGATCAGCTGTGGTGTGAGGACTTCTC AGACAAAGGCAAGGCCTCGTGCCCCTGCCCAGCCCATTCATGGAGCCCTGGGCCTTCTTGGCTTCCATAGATCCTAAGCTCTTGACTGTAGTTTAGCCAG ACTTGTTTTGCTATCTTATAAGCAGTTCAGAATTAGGGAATGCTGGTTTTGAAGAGCAAAGGACAGGTAGTCTAGAGAGGGTCGTCTGGCCTGCTTGCTG GGTCTTTGTAACCCAGCACTTCCTCTTGCCCTCCTGGCTTTATGTTTATGGGGAGAGGACTCAATAGCTCCACCCCTTCTGGCACCAGATGGGGCTTGGT TAGTTTGCAATAAGCACCTTGCAGAGGTTAAAGCCAGCGGGTCCCTAGTCTTAGGCCCAGCCTGCTTGTGTGGGCTCTGGCCTGGCCTGGTGGCTGGCCC AGGGGGCAGCAGTGCTTAGAGCTTCTGCAGGGCTTCTCTTGTTTACACAGCTGCATCAGACAATGCCATTTCTCCCCACCACGGAACCTTCCATCTAAGA TTTCTTCCAGGGAATGCCAGCAATCAGGCAGCACCCAGCTGTGGGGGCAGTGGGGTGGGGGAGACCCACATTGATGACTTTTTTTTTTTCTTTTAATGAA GAAACACCAAAGAAAGCTGTGGAAAGGACCTGCCCCACATGAAAAGGATAAGCCAAGATGGCTGTAAACACAGAGCATTTGAGCTGCCACTCTTGGAGCA CATTGATTTTTCAAAAGCCAGCTCTGTCAGGAAAGGAGGTGCTGTTATGAGCAGCTCTTCCAGTGGGCAAAGAGGACGCCCATAATTTCTTCCATTGCTA GCTCATCTGTGGGACCAATTTGGTGTAAGCAACCTGTGGCCTGCACTTGTGGCCTCGAAGGAAGCACAAACCCTCCATCCACTTCCCATTTCCTCTGCCC TTTTCCACCTCCCCCTTCCATCCCACCAGCTGCCAGTGGCTCCCAGAAAGCCTTATTGAGCCCCTTGTTGACACTTGGGGCTGCGGAGGCCTCTCCCTAC TGGTCTGGCCTTTCCTGAGAGGCAGGTCTTCCGTCCTCAGAGCCTTTCTGGAACAAGGAGAATGCCTGTGCAGGTGGACACACAGGCCTGGCCTGTCGCT CTCACTTGTCTTCCAGCGGGGAGCTTCACGTTGCCGAGTGGAAGAACCATGACCTCCACTTGCTTCCAAGGTGCTAGGGAAGTTTCAGGGTACGCTGGTT CCCCTCTCCAGCTGGAGGCCGAGTTTCTGGGGACTGCAGATTTTTCTACTCTGTGATCGATTCAATGCCCGATGCTTCTGTTTCATTCCCGACCCTTTCT ACTATGCATTTTCCTTTTATCAGGTGTATAAAGTTAAATACTGTGTATTTATCACTAAAAAGTACATGAACTTAAGAGACAACTAAGCCTTTCGTGTTTT TCCACAGGTGTTTAAGCTTCTCTGTACAGTTGAAATAAACAGACAGCAAAATGGTGCCAA

refseq_CAMKK2.F2 (forward primer)
refseq_CAMKK2.R2 (reverse primer)
Single exon skipping

Frame 0

EPGEVEGAASRDRATALQPGQHRETLSQNKQTN KQTNKQKKQRKKNGSGPGAVTHTCNPSTFGGQGG WITRSGIQD*PGQHGETPSLRKIQKLAKYGGRR L*SQLLGRLRQRTA*TWEAEVAVIRDRVTALQR GRQSETPFQKRKKKKKKRESGWSSHWLVACECPY GLEIQEKHIGTGWKSTQLTRGL*TFY*LGG*LS CQLDSLLAVWQGQ*DIPWVARPRRTVQGALQVF VSPLPRTVLLSSWGIQRTLKAPAKWLRCRMGVPQ CAG*SWRMHHVIMCL*PAQQQPGRPPG*AGGQG QQQQRKPEAL*GPAGPLILEHPPGHGVLHCGHR V*AGLCCGPRLGAGPAPGGRWPRGPP*HLRVPGP APPLRSQAVSARAVPGWAGSRWQPGHERTLHLP VPALLTRQLPAVLASAAPAADSGVSPRLHHGYA GLCAAESVYPEG*NWKGLLWCRQVGLQ*K*QYLL CNEGAVQKEADPAGRLSTSPSTPRHPASSWRLH PAQGPH*AGVPGNCHPQEAGPPQCGEAGGGPG* PQ*GPSVHGVRTGQPRARDGSAHPQTTL*RPGPF LLPGSDQRHRVLTLPEDHPP*HQTFQPPGRRRW AHQDR*LWCEQ*IQGQ*RAPLQHRGHARLHGTR VAL*DPQDLLWEGLGCLGHGCDTILLCLWPVPIH GRADHVFTQ*DQESGPGISRPARHS*GLEGPDH PYAGQEPRVEDRGAGNQAAPLGHEAWGGAVAVG G*ELHAGRSD*RGGRELSQTHSQLGNRDPGEDHD T*TLLWEPIRGQPAGGTLTVSAWKLAHEARQRR QPPGHRPAPRGGGGSALVRGSPCVESCWAPAPG SPARMHPLRPEEAMEPE*LPGSLDLACAPRRLWG AAAPRFHSSMSYGNPARV*SLDRHLWLFVFSFV VLKGTKKKKGLDSMTSTVAAGWLDRRV*GVADP NPRAFWDNCFLKRFYAKNPSL*FSEPRQIYQVTV CGV*QLWKGEQKTPAV*GHGGGCCI*EGVGG*M WLLVQTGNHGTFQSRGLKESEQGWPSVAAGSFD ATYL*EGFLNFLWVEL*SGKQKGLEI**VLLEGD FPSLEGYSAAVVGKHFS*KTERVSS*QLLKAGF *DS*PSSGKSLCQITKTSG*NPMLPCTLHGLRL NKNIFQVSN*SEL*STVVQKLFRASRIYFTASGM *SVRADETYARLYIY*QLS*FITCIF*RLLRFC FHFKS*LIVVVNEIPSFWRARWEMLSLDQHTND HPEDLSFPRLHTITLGHPFREDC*NPQHSLGYEE TRAWHRKFPFVAKSPERKGSSF*LPTDIGKFG* GSSVTPSRATGRGV*S*GGGKLEGLCLGRFPAL LSSAWWNLGWGKTAPGNSASPLFPSDQLWCEDFS DKGKASCPCPAHSWSPGPSWLP*ILSS*L*FSQ TCFAIL*AVQN*GMLVLKSKGQVV*RGSSGLLA GSL*PSTSSCPPGFMFMGRGLNSSTPSGTRWGLV SLQ*APCRG*SQRVPSLRPSLLVWALAWPGGWP RGQQCLELLQGFSCLHSCIRQCHFSPPRNLPSK ISSRECQQSGSTQLWGQWGGGDPH**LFFFLLMK KHQRKLWKGPAPHEKDKPRWL*TQSI*AATLGA H*FFKSQLCQERRCCYEQLFQWAKRTPIISSIA SSSVGPIWCKQPVACTCGLEGSTNPPSTSHFLCP FPPPPSIPPAASGSQKALLSPLLTLGAAEASPY WSGLS*EAGLPSSEPFWNKENACAGGHTGLACR SHLSSSGELHVAEWKNHDLHLLPRC*GSFRVRWF PSPAGGRVSGDCRFFYSVIDSMPDASVSFPTLS TMHFPFIRCIKLNTVYLSLKST*T*ETTKPFVF FHRCLSFSVQLK*TDSKMVP

Frame 1

SLGRSRVQRAVIVLLHSSLGNTERPCLKTNKQT NKQTNKKNKEKKMGVGRAR*LTPVIPALSEAKA GGSRGQEFKISLDNMVKPHLYEKYKN*PSMVAGA CNPSYSGD*GRELLEPGRQRLQ*SEIASLHSSV GDRARLRFRKEKKKKKKGSRGGALIGSLHVSVP TA*KYKRSTSERAGNPPS*LEGFEPFINLEVDSP VNSIPFWLFGRVSETSPGSLDPVGRFREPSRSS FLLFPAQCCYPAGGSNAHLRLQQSGSAAGWECP SVLDEAGACTMSSCVSSQPSSNRAAPQDELGGRG SSSSESQKPCEALRGLSSLSIHLGMESFIVVTE CEPGCAVDLGLARDRPLEADGQEVPLDTSGSQA RPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICP SLPYSPVSSPQSSPRLPRRPTVESHHVSITGMQ DCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTY YAMKVLSKKKLIRQAGFPRRPPPRGTRPAPGGCI QPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDD PNEDHLYMVFELVNQGPVMEVPTLKPLSEDQAR FYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDG HIKIADFGVSNEFKGSDALLSNTVGTPAFMAPE SLSETRKIFSGKALDVWAMGVTLYCFVFGQCPF MDERIMCLHSKIKSQALEFPDQPDIAEDLKDLIT RMLDKNPESRIVVPEIKLHPWVTRHGAEPLPSE DENCTLVEVTEEEVENSVKHIPSLATVILVKTM IRKRSFGNPFEGSRREERSLSAPGNLLTKQGSED NLQGTDPPPVGEEEVLL*EAVPAWKVAGPPPPA PPHACIHCGRRRPWSPSSCLDRSTSHARRVASG GLLHRVSIAACPTETQHVCRASIVISGYLFFPLL F*RGQKKKKDLTP*RRPWPLAGWTGGCEELQTQ THVHFGTIAF*NVFMPKILHCDFQNHVRYTK*L CVGFDNCGKASRKLRRSEAMEVVAAFERE*GARC GS*CKPETMAPSRAVVSRSQSRAGPQ*LQGALM QLICKKDF*IFYG*NCSQENRKGLKFNKCCWKG IFQAWKGIQQLWWGNISPERLNVFLHDSCSKQVS EIADRALVNLFVKLRKLQGEILCFHVHYMA*D* TKTFFKSLTRVNSRAQ*FRNYLELPGYISQLQA CDQLEPMKPMPACIYISSLASS*PVYSKDC*GFV FILNPS*LLWSMKYPVSGGPGGKCFHWTNTQMI ILRI*ASLDSTQ*PWGTLLEKTVETHSTRWGMR KPGLGTGSSPL*LKVQKERVHLFDFQLILGSLVE VQV*LLPEPQVGECEVEGEESWKDSALGDSQLC FPALGGIWAGERRHRETLLPHCFHLISCGVRTS QTKARPRAPAQPIHGALGLLGFHRS*ALDCSLAR LVLLSYKQFRIRECWF*RAKDR*SREGRLACLL GLCNPALPLALLALCLWGEDSIAPPLLAPDGAW LVCNKHLAEVKASGSLVLGPACLCGLWPGLVAGP GGSSA*SFCRASLVYTAASDNAISPHHGTFHLR FLPGNASNQAAPSCGGSGVGETHIDDFFFFF** RNTKESCGKDLPHMKRISQDGCKHRAFELPLLEH IDFSKASSVRKGGAVMSSSSSGQRGRP*FLPLL AHLWDQFGVSNLWPALVASKEAQTLHPLPISSA LFHLPLPSHQLPVAPRKPY*APC*HLGLRRPLPT GLAFPERQVFRPQSLSGTRRMPVQVDTQAWPVA LTCLPAGSFTLPSGRTMTSTCFQGAREVSGYAG SPLQLEAEFLGTADFSTL*SIQCPMLLFHSRPFL LCIFLLSGV*S*ILCIYH*KVHELKRQLSLSCF STGV*ASLYS*NKQTAKWCQ

Frame 2

AWGGRGCSEP*SCYCTPAWATQRDPVSKQTNKQ TNKQTKKTKKKKWEWAGRGDSHL*SQHFRRPRR VDHEVRNSRLAWTTW*NPISTKNTKISQVWWPA PVIPATRETEAENCLNLGGRGCSDPRSRHCTPAW ATERDSVSEKKKKKKKKGVGVELSLARCM*VSL RPRNTREAHRNGLEIHPVN*RALNLLLTWRLTL LSTRFPFGCLAGSVRHPLGRSTP*DGSGSPPGLR FSSSPHSAVIQLGDPTHT*GSSKVAPLPDGSAP VCWMKLAHAPCHHVSLASPAATGPPPRMSWGAG AAAAAKARSPVRPCGASHP*ASTWAWSPSLWSPS VSRAVLWTSAWRGTGPWRPMAKRSPLTPPGPRP GPTSPVASCLCKSGPRVGWQPVAAWT*TDAASA RPCPTHPSAPRSPRLGCPGGRQWSLTTSPSRVCR TVCS*ISIP*RMKLERAPMVSSSWPTMKMTIPT MQ*RCCPKRS*SGRPAFHVALHPEAPGQLLEAA SSPGAPLSRCTRKLPSSRSWTTPMW*SWWRSWMT PMRTICTWCSNWSTKGP*WKCPPSNHSLKTRPV STSRI*SKASSTYTTRRSSTVTSNLPTSWSEKM GTSRSLTLV*AMNSRAVTRSSPTPWARPPSWHPS RSLRPARSSLGRPWMFGPWV*HYTALSLASAHS WTSGSCVYTVRSRVRPWNFQTSPT*LRT*RT*S PVCWTRTPSRGSWCRKSSCTPGSRGMGRSRCRRR MRTARWSK*LKRRSRTQSNTFPAWQP*SW*RP* YVNAPLGTHSRAAGGRNAHCQRLETCSRSKAAK TTSRAPTRPPWGRRKCSCERQSLRGKLLGPRPRL PRTHASTAAGGGHGARVAAWIARPRMRAASPLG GCCTAFP*QHVLRKPSTCVEPRSSSLVICFFLC CFKGDKKKKRT*LHDVDRGRWLAGQAGVRSCRPK PTCILGQLLFKTFLCQKSFIVIFRTTSDIPSDC VWGLTTVERRAENSGGLRPWRWLLHLRGSRGLD VAPSANRKPWHLPEPWSQGVRAGLALSSCREL*C NLFVRRIFKFFMGRIVVRKTERA*NLISAAGRG FSKPGRVFSSCGGETFLLKD*TCFFMTAAQSRF LR*LTELW*ISLSNYENFRVKSYASMYITWLKIK QKHFSSL*LE*TLEHSSSETI*SFQDIFHSFRH VIS*SR*NLCPPVYILAA*LVHNLYILKTAKVL FSF*ILADCCGQ*NTQFLEGQVGNAFTGPTHK*S S*GSELP*TPHNNLGAPF*RRLLKPTALVGV*G NQGLAQEVPLCS*KSRKKGFIFLTSN*YWEVWL RFKCDSFQSHR*GSVKLRGRKAGRTLPWEIPSSA FQRLVESGLGKDGTGKLCFPIVSI*SAVV*GLL RQRQGLVPLPSPFMEPWAFLASIDPKLLTVV*P DLFCYLISSSELGNAGFEEQRTGSLERVVWPACW VFVTQHFLLPSWLYVYGERTQ*LHPFWHQMGLG *FAISTLQRLKPAGP*S*AQPACVGSGLAWWLA QGAAVLRASAGLLLFTQLHQTMPFLPTTEPSI*D FFQGMPAIRQHPAVGAVGWGRPTLMTFFFSFNE ETPKKAVERTCPT*KG*AKMAVNTEHLSCHSWS TLIFQKPALSGKEVLL*AALPVGKEDAHNFFHC* LICGTNLV*ATCGLHLWPRRKHKPSIHFPFPLP FSTSPFHPTSCQWLPESLIEPLVDTWGCGGLSL LVWPFLRGRSSVLRAFLEQGECLCRWTHRPGLSL SLVFQRGASRCRVEEP*PPLASKVLGKFQGTLV PLSSWRPSFWGLQIFLLCDRFNARCFCFIPDPF YYAFSFYQVYKVKYCVFITKKYMNLRDN*AFRVF PQVFKLLCTVEINRQQNGA

Sequence NM_153500

GAGCCTGGGGAGGTCGAGGGTGCAGCGAGCCGTGATCGTGCTACTGCACTCCAGCCTGGGCAACACAGAGAGACCCTGTCTCAAAACAAACAAACAAACA AACAAACAAACAAACAAAAAAAACAAAGAAAAAAAAATGGGAGTGGGCCGGGCGCGGTGACTCACACCTGTAATCCCAGCACTTTCGGAGGCCAAGGCGG GTGGATCACGAGGTCAGGAATTCAAGATTAGCCTGGACAACATGGTGAAACCCCATCTCTACGAAAAATACAAAAATTAGCCAAGTATGGTGGCCGGCGC CTGTAATCCCAGCTACTCGGGAGACTGAGGCAGAGAACTGCTTGAACCTGGGAGGCAGAGGTTGCAGTGATCCGAGATCGCGTCACTGCACTCCAGCGTG GGCGACAGAGCGAGACTCCGTTTCAGAAAAGAAAAAAAAAAAAAAAAAAAAGGGAGTCGGGGTGGAGCTCTCATTGGCTCGTTGCATGTGAGTGTCCCTA CGGCCTAGAAATACAAGAGAAGCACATCGGAACGGGCTGGAAATCCACCCAGTTAACTAGAGGGCTTTGAACCTTTTATTAACTTGGAGGTTGACTCTCC TGTCAACTCGATTCCCTTTTGGCTGTTTGGCAGGGTCAGTGAGACATCCCCTGGGTCGCTCGACCCCGTAGGACGGTTCAGGGAGCCCTCCAGGTCTTCG TTTCTCCTCTTCCCCGCACAGTGCTGTTATCCAGCTGGGGGATCCAACGCACACTTAAGGCTCCAGCAAAGTGGCTCCGCTGCCGGATGGGAGTGCCCCA GTGTGCTGGATGAAGCTGGCGCATGCACCATGTCATCATGTGTCTCTAGCCAGCCCAGCAGCAACCGGGCCGCCCCCCAGGATGAGCTGGGGGGCAGGGG CAGCAGCAGCAGCGAAAGCCAGAAGCCCTGTGAGGCCCTGCGGGGCCTCTCATCCTTGAGCATCCACCTGGGCATGGAGTCCTTCATTGTGGTCACCGAG TGTGAGCCGGGCTGTGCTGTGGACCTCGGCTTGGCGCGGGACCGGCCCCTGGAGGCCGATGGCCAAGAGGTCCCCCTTGACACCTCCGGGTCCCAGGCCC GGCCCCACCTCTCCGGTCGCAAGCTGTCTCTGCAAGAGCGGTCCCAGGGTGGGCTGGCAGCCGGTGGCAGCCTGGACATGAACGGACGCTGCATCTGCCC GTCCCTGCCCTACTCACCCGTCAGCTCCCCGCAGTCCTCGCCTCGGCTGCCCCGGCGGCCGACAGTGGAGTCTCACCACGTCTCCATCACGGGTATGCAG GACTGTGTGCAGCTGAATCAGTATACCCTGAAGGATGAAATTGGAAAGGGCTCCTATGGTGTCGTCAAGTTGGCCTACAATGAAAATGACAATACCTACT ATGCAATGAAGGTGCTGTCCAAAAAGAAGCTGATCCGGCAGGCCGGCTTTCCACGTCGCCCTCCACCCCGAGGCACCCGGCCAGCTCCTGGAGGCTGCAT CCAGCCCAGGGGCCCCATTGAGCAGGTGTACCAGGAAATTGCCATCCTCAAGAAGCTGGACCACCCCAATGTGGTGAAGCTGGTGGAGGTCCTGGATGAC CCCAATGAGGACCATCTGTACATGGTGTTCGAACTGGTCAACCAAGGGCCCGTGATGGAAGTGCCCACCCTCAAACCACTCTCTGAAGACCAGGCCCGTT TCTACTTCCAGGATCTGATCAAAGGCATCGAGTACTTACACTACCAGAAGATCATCCACCGTGACATCAAACCTTCCAACCTCCTGGTCGGAGAAGATGG GCACATCAAGATCGCTGACTTTGGTGTGAGCAATGAATTCAAGGGCAGTGACGCGCTCCTCTCCAACACCGTGGGCACGCCCGCCTTCATGGCACCCGAG TCGCTCTCTGAGACCCGCAAGATCTTCTCTGGGAAGGCCTTGGATGTTTGGGCCATGGGTGTGACACTATACTGCTTTGTCTTTGGCCAGTGCCCATTCA TGGACGAGCGGATCATGTGTTTACACAGTAAGATCAAGAGTCAGGCCCTGGAATTTCCAGACCAGCCCGACATAGCTGAGGACTTGAAGGACCTGATCAC CCGTATGCTGGACAAGAACCCCGAGTCGAGGATCGTGGTGCCGGAAATCAAGATCCTGGTGAAGACCATGATACGTAAACGCTCCTTTGGGAACCCATTC GAGGGCAGCCGGCGGGAGGAACGCTCACTGTCAGCGCCTGGAAACTTGCTCACGAAGCAAGGCAGCGAAGACAACCTCCAGGGCACCGACCCGCCCCCCG TGGGGGAGGAGGAAGTGCTCTTGTGAGAGGCAGTCCCTGCGTGGAAAGTTGCTGGGCCCCCGCCCCCGGCTCCCCCGCACGCATGCATCCACTGCGGCCG GAGGAGGCCATGGAGCCCGAGTAGCTGCCTGGATCGCTCGACCTCGCATGCGCGCCGCGTCGCCTCTGGGGGGCTGCTGCACCGCGTTTCCATAGCAGCA TGTCCTACGGAAACCCAGCACGTGTGTAGAGCCTCGATCGTCATCTCTGGTTATTTGTTTTTTCCTTTGTTGTTTTAAAGGGGACAAAAAAAAAAAAAGG ACTTGACTCCATGACGTCGACCGTGGCCGCTGGCTGGCTGGACAGGCGGGTGTGAGGAGTTGCAGACCCAAACCCACGTGCATTTTGGGACAATTGCTTT TTAAAACGTTTTTATGCCAAAAATCCTTCATTGTGATTTTCAGAACCACGTCAGATATACCAAGTGACTGTGTGTGGGGTTTGACAACTGTGGAAAGGCG AGCAGAAAACTCCGGCGGTCTGAGGCCATGGAGGTGGTTGCTGCATTTGAGAGGGAGTAGGGGGCTAGATGTGGCTCCTAGTGCAAACCGGAAACCATGG CACCTTCCAGAGCCGTGGTCTCAAGGAGTCAGAGCAGGGCTGGCCCTCAGTAGCTGCAGGGAGCTTTGATGCAACTTATTTGTAAGAAGGATTTTTAAAT TTTTTATGGGTAGAATTGTAGTCAGGAAAACAGAAAGGGCTTGAAATTTAATAAGTGCTGCTGGAAGGGGATTTTCCAAGCCTGGAAGGGTATTCAGCAG CTGTGGTGGGGAAACATTTCTCCTGAAAGACTGAACGTGTTTCTTCATGACAGCTGCTCAAAGCAGGTTTCTGAGATAGCTGACCGAGCTCTGGTAAATC TCTTTGTCAAATTACGAAAACTTCAGGGTGAAATCCTATGCTTCCATGTACATTACATGGCTTAAGATTAAACAAAAACATTTTTCAAGTCTCTAACTAG AGTGAACTCTAGAGCACAGTAGTTCAGAAACTATTTAGAGCTTCCAGGATATATTTCACAGCTTCAGGCATGTGATCAGTTAGAGCCGATGAAACCTATG CCCGCCTGTATATATATTAGCAGCTTAGCTAGTTCATAACCTGTATATTCTAAAGACTGCTAAGGTTTTGTTTTCATTTTAAATCCTAGCTGATTGTTGT GGTCAATGAAATACCCAGTTTCTGGAGGGCCAGGTGGGAAATGCTTTCACTGGACCAACACACAAATGATCATCCTGAGGATCTGAGCTTCCCTAGACTC CACACAATAACCTTGGGGCACCCTTTTAGAGAAGACTGTTGAAACCCACAGCACTCGTTGGGGTATGAGGAAACCAGGGCTTGGCACAGGAAGTTCCCCT TTGTAGCTAAAAGTCCAGAAAGAAAGGGTTCATCTTTTTGACTTCCAACTGATATTGGGAAGTTTGGTTGAGGTTCAAGTGTGACTCCTTCCAGAGCCAC AGGTAGGGGAGTGTGAAGTTGAGGGGGAGGAAAGCTGGAAGGACTCTGCCTTGGGAGATTCCCAGCTCTGCTTTCCAGCGCTTGGTGGAATCTGGGCTGG GGAAAGACGGCACCGGGAAACTCTGCTTCCCCATTGTTTCCATCTGATCAGCTGTGGTGTGAGGACTTCTCAGACAAAGGCAAGGCCTCGTGCCCCTGCC CAGCCCATTCATGGAGCCCTGGGCCTTCTTGGCTTCCATAGATCCTAAGCTCTTGACTGTAGTTTAGCCAGACTTGTTTTGCTATCTTATAAGCAGTTCA GAATTAGGGAATGCTGGTTTTGAAGAGCAAAGGACAGGTAGTCTAGAGAGGGTCGTCTGGCCTGCTTGCTGGGTCTTTGTAACCCAGCACTTCCTCTTGC CCTCCTGGCTTTATGTTTATGGGGAGAGGACTCAATAGCTCCACCCCTTCTGGCACCAGATGGGGCTTGGTTAGTTTGCAATAAGCACCTTGCAGAGGTT AAAGCCAGCGGGTCCCTAGTCTTAGGCCCAGCCTGCTTGTGTGGGCTCTGGCCTGGCCTGGTGGCTGGCCCAGGGGGCAGCAGTGCTTAGAGCTTCTGCA GGGCTTCTCTTGTTTACACAGCTGCATCAGACAATGCCATTTCTCCCCACCACGGAACCTTCCATCTAAGATTTCTTCCAGGGAATGCCAGCAATCAGGC AGCACCCAGCTGTGGGGGCAGTGGGGTGGGGGAGACCCACATTGATGACTTTTTTTTTTTCTTTTAATGAAGAAACACCAAAGAAAGCTGTGGAAAGGAC CTGCCCCACATGAAAAGGATAAGCCAAGATGGCTGTAAACACAGAGCATTTGAGCTGCCACTCTTGGAGCACATTGATTTTTCAAAAGCCAGCTCTGTCA GGAAAGGAGGTGCTGTTATGAGCAGCTCTTCCAGTGGGCAAAGAGGACGCCCATAATTTCTTCCATTGCTAGCTCATCTGTGGGACCAATTTGGTGTAAG CAACCTGTGGCCTGCACTTGTGGCCTCGAAGGAAGCACAAACCCTCCATCCACTTCCCATTTCCTCTGCCCTTTTCCACCTCCCCCTTCCATCCCACCAG CTGCCAGTGGCTCCCAGAAAGCCTTATTGAGCCCCTTGTTGACACTTGGGGCTGCGGAGGCCTCTCCCTACTGGTCTGGCCTTTCCTGAGAGGCAGGTCT TCCGTCCTCAGAGCCTTTCTGGAACAAGGAGAATGCCTGTGCAGGTGGACACACAGGCCTGGCCTGTCGCTCTCACTTGTCTTCCAGCGGGGAGCTTCAC GTTGCCGAGTGGAAGAACCATGACCTCCACTTGCTTCCAAGGTGCTAGGGAAGTTTCAGGGTACGCTGGTTCCCCTCTCCAGCTGGAGGCCGAGTTTCTG GGGACTGCAGATTTTTCTACTCTGTGATCGATTCAATGCCCGATGCTTCTGTTTCATTCCCGACCCTTTCTACTATGCATTTTCCTTTTATCAGGTGTAT AAAGTTAAATACTGTGTATTTATCACTAAAAAGTACATGAACTTAAGAGACAACTAAGCCTTTCGTGTTTTTCCACAGGTGTTTAAGCTTCTCTGTACAG TTGAAATAAACAGACAGCAAAATGGTGCCAA

refseq_CAMKK2.F2 (forward primer)
refseq_CAMKK2.R2 (reverse primer)
Single exon skipping

Frame 0

EPGEVEGAASRDRATALQPGQHRETLSQNKQTN KQTNKQKKQRKKNGSGPGAVTHTCNPSTFGGQGG WITRSGIQD*PGQHGETPSLRKIQKLAKYGGRR L*SQLLGRLRQRTA*TWEAEVAVIRDRVTALQR GRQSETPFQKRKKKKKKRESGWSSHWLVACECPY GLEIQEKHIGTGWKSTQLTRGL*TFY*LGG*LS CQLDSLLAVWQGQ*DIPWVARPRRTVQGALQVF VSPLPRTVLLSSWGIQRTLKAPAKWLRCRMGVPQ CAG*SWRMHHVIMCL*PAQQQPGRPPG*AGGQG QQQQRKPEAL*GPAGPLILEHPPGHGVLHCGHR V*AGLCCGPRLGAGPAPGGRWPRGPP*HLRVPGP APPLRSQAVSARAVPGWAGSRWQPGHERTLHLP VPALLTRQLPAVLASAAPAADSGVSPRLHHGYA GLCAAESVYPEG*NWKGLLWCRQVGLQ*K*QYLL CNEGAVQKEADPAGRLSTSPSTPRHPASSWRLH PAQGPH*AGVPGNCHPQEAGPPQCGEAGGGPG* PQ*GPSVHGVRTGQPRARDGSAHPQTTL*RPGPF LLPGSDQRHRVLTLPEDHPP*HQTFQPPGRRRW AHQDR*LWCEQ*IQGQ*RAPLQHRGHARLHGTR VAL*DPQDLLWEGLGCLGHGCDTILLCLWPVPIH GRADHVFTQ*DQESGPGISRPARHS*GLEGPDH PYAGQEPRVEDRGAGNQDPGEDHDT*TLLWEPI RGQPAGGTLTVSAWKLAHEARQRRQPPGHRPAPR GGGGSALVRGSPCVESCWAPAPGSPARMHPLRP EEAMEPE*LPGSLDLACAPRRLWGAAAPRFHSS MSYGNPARV*SLDRHLWLFVFSFVVLKGTKKKKG LDSMTSTVAAGWLDRRV*GVADPNPRAFWDNCF LKRFYAKNPSL*FSEPRQIYQVTVCGV*QLWKG EQKTPAV*GHGGGCCI*EGVGG*MWLLVQTGNHG TFQSRGLKESEQGWPSVAAGSFDATYL*EGFLN FLWVEL*SGKQKGLEI**VLLEGDFPSLEGYSA AVVGKHFS*KTERVSS*QLLKAGF*DS*PSSGKS LCQITKTSG*NPMLPCTLHGLRLNKNIFQVSN* SEL*STVVQKLFRASRIYFTASGM*SVRADETY ARLYIY*QLS*FITCIF*RLLRFCFHFKS*LIVV VNEIPSFWRARWEMLSLDQHTNDHPEDLSFPRL HTITLGHPFREDC*NPQHSLGYEETRAWHRKFP FVAKSPERKGSSF*LPTDIGKFG*GSSVTPSRAT GRGV*S*GGGKLEGLCLGRFPALLSSAWWNLGW GKTAPGNSASPLFPSDQLWCEDFSDKGKASCPC PAHSWSPGPSWLP*ILSS*L*FSQTCFAIL*AVQ N*GMLVLKSKGQVV*RGSSGLLAGSL*PSTSSC PPGFMFMGRGLNSSTPSGTRWGLVSLQ*APCRG *SQRVPSLRPSLLVWALAWPGGWPRGQQCLELLQ GFSCLHSCIRQCHFSPPRNLPSKISSRECQQSG STQLWGQWGGGDPH**LFFFLLMKKHQRKLWKG PAPHEKDKPRWL*TQSI*AATLGAH*FFKSQLCQ ERRCCYEQLFQWAKRTPIISSIASSSVGPIWCK QPVACTCGLEGSTNPPSTSHFLCPFPPPPSIPP AASGSQKALLSPLLTLGAAEASPYWSGLS*EAGL PSSEPFWNKENACAGGHTGLACRSHLSSSGELH VAEWKNHDLHLLPRC*GSFRVRWFPSPAGGRVS GDCRFFYSVIDSMPDASVSFPTLSTMHFPFIRCI KLNTVYLSLKST*T*ETTKPFVFFHRCLSFSVQ LK*TDSKMVP

Frame 1

SLGRSRVQRAVIVLLHSSLGNTERPCLKTNKQT NKQTNKKNKEKKMGVGRAR*LTPVIPALSEAKA GGSRGQEFKISLDNMVKPHLYEKYKN*PSMVAGA CNPSYSGD*GRELLEPGRQRLQ*SEIASLHSSV GDRARLRFRKEKKKKKKGSRGGALIGSLHVSVP TA*KYKRSTSERAGNPPS*LEGFEPFINLEVDSP VNSIPFWLFGRVSETSPGSLDPVGRFREPSRSS FLLFPAQCCYPAGGSNAHLRLQQSGSAAGWECP SVLDEAGACTMSSCVSSQPSSNRAAPQDELGGRG SSSSESQKPCEALRGLSSLSIHLGMESFIVVTE CEPGCAVDLGLARDRPLEADGQEVPLDTSGSQA RPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICP SLPYSPVSSPQSSPRLPRRPTVESHHVSITGMQ DCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTY YAMKVLSKKKLIRQAGFPRRPPPRGTRPAPGGCI QPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDD PNEDHLYMVFELVNQGPVMEVPTLKPLSEDQAR FYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDG HIKIADFGVSNEFKGSDALLSNTVGTPAFMAPE SLSETRKIFSGKALDVWAMGVTLYCFVFGQCPF MDERIMCLHSKIKSQALEFPDQPDIAEDLKDLIT RMLDKNPESRIVVPEIKILVKTMIRKRSFGNPF EGSRREERSLSAPGNLLTKQGSEDNLQGTDPPP VGEEEVLL*EAVPAWKVAGPPPPAPPHACIHCGR RRPWSPSSCLDRSTSHARRVASGGLLHRVSIAA CPTETQHVCRASIVISGYLFFPLLF*RGQKKKK DLTP*RRPWPLAGWTGGCEELQTQTHVHFGTIAF *NVFMPKILHCDFQNHVRYTK*LCVGFDNCGKA SRKLRRSEAMEVVAAFERE*GARCGS*CKPETM APSRAVVSRSQSRAGPQ*LQGALMQLICKKDF*I FYG*NCSQENRKGLKFNKCCWKGIFQAWKGIQQ LWWGNISPERLNVFLHDSCSKQVSEIADRALVN LFVKLRKLQGEILCFHVHYMA*D*TKTFFKSLTR VNSRAQ*FRNYLELPGYISQLQACDQLEPMKPM PACIYISSLASS*PVYSKDC*GFVFILNPS*LL WSMKYPVSGGPGGKCFHWTNTQMIILRI*ASLDS TQ*PWGTLLEKTVETHSTRWGMRKPGLGTGSSP L*LKVQKERVHLFDFQLILGSLVEVQV*LLPEP QVGECEVEGEESWKDSALGDSQLCFPALGGIWAG ERRHRETLLPHCFHLISCGVRTSQTKARPRAPA QPIHGALGLLGFHRS*ALDCSLARLVLLSYKQF RIRECWF*RAKDR*SREGRLACLLGLCNPALPLA LLALCLWGEDSIAPPLLAPDGAWLVCNKHLAEV KASGSLVLGPACLCGLWPGLVAGPGGSSA*SFC RASLVYTAASDNAISPHHGTFHLRFLPGNASNQA APSCGGSGVGETHIDDFFFFF**RNTKESCGKD LPHMKRISQDGCKHRAFELPLLEHIDFSKASSV RKGGAVMSSSSSGQRGRP*FLPLLAHLWDQFGVS NLWPALVASKEAQTLHPLPISSALFHLPLPSHQ LPVAPRKPY*APC*HLGLRRPLPTGLAFPERQV FRPQSLSGTRRMPVQVDTQAWPVALTCLPAGSFT LPSGRTMTSTCFQGAREVSGYAGSPLQLEAEFL GTADFSTL*SIQCPMLLFHSRPFLLCIFLLSGV *S*ILCIYH*KVHELKRQLSLSCFSTGV*ASLYS *NKQTAKWCQ

Frame 2

AWGGRGCSEP*SCYCTPAWATQRDPVSKQTNKQ TNKQTKKTKKKKWEWAGRGDSHL*SQHFRRPRR VDHEVRNSRLAWTTW*NPISTKNTKISQVWWPA PVIPATRETEAENCLNLGGRGCSDPRSRHCTPAW ATERDSVSEKKKKKKKKGVGVELSLARCM*VSL RPRNTREAHRNGLEIHPVN*RALNLLLTWRLTL LSTRFPFGCLAGSVRHPLGRSTP*DGSGSPPGLR FSSSPHSAVIQLGDPTHT*GSSKVAPLPDGSAP VCWMKLAHAPCHHVSLASPAATGPPPRMSWGAG AAAAAKARSPVRPCGASHP*ASTWAWSPSLWSPS VSRAVLWTSAWRGTGPWRPMAKRSPLTPPGPRP GPTSPVASCLCKSGPRVGWQPVAAWT*TDAASA RPCPTHPSAPRSPRLGCPGGRQWSLTTSPSRVCR TVCS*ISIP*RMKLERAPMVSSSWPTMKMTIPT MQ*RCCPKRS*SGRPAFHVALHPEAPGQLLEAA SSPGAPLSRCTRKLPSSRSWTTPMW*SWWRSWMT PMRTICTWCSNWSTKGP*WKCPPSNHSLKTRPV STSRI*SKASSTYTTRRSSTVTSNLPTSWSEKM GTSRSLTLV*AMNSRAVTRSSPTPWARPPSWHPS RSLRPARSSLGRPWMFGPWV*HYTALSLASAHS WTSGSCVYTVRSRVRPWNFQTSPT*LRT*RT*S PVCWTRTPSRGSWCRKSRSW*RP*YVNAPLGTHS RAAGGRNAHCQRLETCSRSKAAKTTSRAPTRPP WGRRKCSCERQSLRGKLLGPRPRLPRTHASTAA GGGHGARVAAWIARPRMRAASPLGGCCTAFP*QH VLRKPSTCVEPRSSSLVICFFLCCFKGDKKKKR T*LHDVDRGRWLAGQAGVRSCRPKPTCILGQLL FKTFLCQKSFIVIFRTTSDIPSDCVWGLTTVERR AENSGGLRPWRWLLHLRGSRGLDVAPSANRKPW HLPEPWSQGVRAGLALSSCREL*CNLFVRRIFK FFMGRIVVRKTERA*NLISAAGRGFSKPGRVFSS CGGETFLLKD*TCFFMTAAQSRFLR*LTELW*I SLSNYENFRVKSYASMYITWLKIKQKHFSSL*L E*TLEHSSSETI*SFQDIFHSFRHVIS*SR*NLC PPVYILAA*LVHNLYILKTAKVLFSF*ILADCC GQ*NTQFLEGQVGNAFTGPTHK*SS*GSELP*T PHNNLGAPF*RRLLKPTALVGV*GNQGLAQEVPL CS*KSRKKGFIFLTSN*YWEVWLRFKCDSFQSH R*GSVKLRGRKAGRTLPWEIPSSAFQRLVESGL GKDGTGKLCFPIVSI*SAVV*GLLRQRQGLVPLP SPFMEPWAFLASIDPKLLTVV*PDLFCYLISSS ELGNAGFEEQRTGSLERVVWPACWVFVTQHFLL PSWLYVYGERTQ*LHPFWHQMGLG*FAISTLQRL KPAGP*S*AQPACVGSGLAWWLAQGAAVLRASA GLLLFTQLHQTMPFLPTTEPSI*DFFQGMPAIR QHPAVGAVGWGRPTLMTFFFSFNEETPKKAVERT CPT*KG*AKMAVNTEHLSCHSWSTLIFQKPALS GKEVLL*AALPVGKEDAHNFFHC*LICGTNLV* ATCGLHLWPRRKHKPSIHFPFPLPFSTSPFHPTS CQWLPESLIEPLVDTWGCGGLSLLVWPFLRGRS SVLRAFLEQGECLCRWTHRPGLSLSLVFQRGAS RCRVEEP*PPLASKVLGKFQGTLVPLSSWRPSFW GLQIFLLCDRFNARCFCFIPDPFYYAFSFYQVY KVKYCVFITKKYMNLRDN*AFRVFPQVFKLLCT VEINRQQNGA

Sequence NM_006549

GAGCCTGGGGAGGTCGAGGGTGCAGCGAGCCGTGATCGTGCTACTGCACTCCAGCCTGGGCAACACAGAGAGACCCTGTCTCAAAACAAACAAACAAACA AACAAACAAACAAACAAAAAAAACAAAGAAAAAAAAATGGGAGTGGGCCGGGCGCGGTGACTCACACCTGTAATCCCAGCACTTTCGGAGGCCAAGGCGG GTGGATCACGAGGTCAGGAATTCAAGATTAGCCTGGACAACATGGTGAAACCCCATCTCTACGAAAAATACAAAAATTAGCCAAGTATGGTGGCCGGCGC CTGTAATCCCAGCTACTCGGGAGACTGAGGCAGAGAACTGCTTGAACCTGGGAGGCAGAGGTTGCAGTGATCCGAGATCGCGTCACTGCACTCCAGCGTG GGCGACAGAGCGAGACTCCGTTTCAGAAAAGAAAAAAAAAAAAAAAAAAAAGGGAGTCGGGGTGGAGCTCTCATTGGCTCGTTGCATGTGAGTGTCCCTA CGGCCTAGAAATACAAGAGAAGCACATCGGAACGGGCTGGAAATCCACCCAGTTAACTAGAGGGCTTTGAACCTTTTATTAACTTGGAGGTTGACTCTCC TGTCAACTCGATTCCCTTTTGGCTGTTTGGCAGGGTCAGTGAGACATCCCCTGGGTCGCTCGACCCCGTAGGACGGTTCAGGGAGCCCTCCAGGTCTTCG TTTCTCCTCTTCCCCGCACAGTGCTGTTATCCAGCTGGGGGATCCAACGCACACTTAAGGCTCCAGCAAAGTGGCTCCGCTGCCGGATGGGAGTGCCCCA GTGTGCTGGATGAAGCTGGCGCATGCACCATGTCATCATGTGTCTCTAGCCAGCCCAGCAGCAACCGGGCCGCCCCCCAGGATGAGCTGGGGGGCAGGGG CAGCAGCAGCAGCGAAAGCCAGAAGCCCTGTGAGGCCCTGCGGGGCCTCTCATCCTTGAGCATCCACCTGGGCATGGAGTCCTTCATTGTGGTCACCGAG TGTGAGCCGGGCTGTGCTGTGGACCTCGGCTTGGCGCGGGACCGGCCCCTGGAGGCCGATGGCCAAGAGGTCCCCCTTGACACCTCCGGGTCCCAGGCCC GGCCCCACCTCTCCGGTCGCAAGCTGTCTCTGCAAGAGCGGTCCCAGGGTGGGCTGGCAGCCGGTGGCAGCCTGGACATGAACGGACGCTGCATCTGCCC GTCCCTGCCCTACTCACCCGTCAGCTCCCCGCAGTCCTCGCCTCGGCTGCCCCGGCGGCCGACAGTGGAGTCTCACCACGTCTCCATCACGGGTATGCAG GACTGTGTGCAGCTGAATCAGTATACCCTGAAGGATGAAATTGGAAAGGGCTCCTATGGTGTCGTCAAGTTGGCCTACAATGAAAATGACAATACCTACT ATGCAATGAAGGTGCTGTCCAAAAAGAAGCTGATCCGGCAGGCCGGCTTTCCACGTCGCCCTCCACCCCGAGGCACCCGGCCAGCTCCTGGAGGCTGCAT CCAGCCCAGGGGCCCCATTGAGCAGGTGTACCAGGAAATTGCCATCCTCAAGAAGCTGGACCACCCCAATGTGGTGAAGCTGGTGGAGGTCCTGGATGAC CCCAATGAGGACCATCTGTACATGGTGTTCGAACTGGTCAACCAAGGGCCCGTGATGGAAGTGCCCACCCTCAAACCACTCTCTGAAGACCAGGCCCGTT TCTACTTCCAGGATCTGATCAAAGGCATCGAGTACTTACACTACCAGAAGATCATCCACCGTGACATCAAACCTTCCAACCTCCTGGTCGGAGAAGATGG GCACATCAAGATCGCTGACTTTGGTGTGAGCAATGAATTCAAGGGCAGTGACGCGCTCCTCTCCAACACCGTGGGCACGCCCGCCTTCATGGCACCCGAG TCGCTCTCTGAGACCCGCAAGATCTTCTCTGGGAAGGCCTTGGATGTTTGGGCCATGGGTGTGACACTATACTGCTTTGTCTTTGGCCAGTGCCCATTCA TGGACGAGCGGATCATGTGTTTACACAGTAAGATCAAGAGTCAGGCCCTGGAATTTCCAGACCAGCCCGACATAGCTGAGGACTTGAAGGACCTGATCAC CCGTATGCTGGACAAGAACCCCGAGTCGAGGATCGTGGTGCCGGAAATCAAGCTGCACCCCTGGGTCACGAGGCATGGGGCGGAGCCGTTGCCGTCGGAG GATGAGAACTGCACGCTGGTCGAAGTGACTGAAGAGGAGGTCGAGAACTCAGTCAAACACATTCCCAGCTTGGCAACCGTGATCCTGGTGAAGACCATGA TACGTAAACGCTCCTTTGGGAACCCATTCGAGGGCAGCCGGCGGGAGGAACGCTCACTGTCAGCGCCTGGAAACTTGCTCACCAAAAAACCAACCAGGGA ATGTGAGTCCCTGTCTGAGCTCAAGGAAGCAAGGCAGCGAAGACAACCTCCAGGGCACCGACCCGCCCCCCGTGGGGGAGGAGGAAGTGCTCTTGTGAGA GGCAGTCCCTGCGTGGAAAGTTGCTGGGCCCCCGCCCCCGGCTCCCCCGCACGCATGCATCCACTGCGGCCGGAGGAGGCCATGGAGCCCGAGTAGCTGC CTGGATCGCTCGACCTCGCATGCGCGCCGCGTCGCCTCTGGGGGGCTGCTGCACCGCGTTTCCATAGCAGCATGTCCTACGGAAACCCAGCACGTGTGTA GAGCCTCGATCGTCATCTCTGGTTATTTGTTTTTTCCTTTGTTGTTTTAAAGGGGACAAAAAAAAAAAAAGGACTTGACTCCATGACGTCGACCGTGGCC GCTGGCTGGCTGGACAGGCGGGTGTGAGGAGTTGCAGACCCAAACCCACGTGCATTTTGGGACAATTGCTTTTTAAAACGTTTTTATGCCAAAAATCCTT CATTGTGATTTTCAGAACCACGTCAGATATACCAAGTGACTGTGTGTGGGGTTTGACAACTGTGGAAAGGCGAGCAGAAAACTCCGGCGGTCTGAGGCCA TGGAGGTGGTTGCTGCATTTGAGAGGGAGTAGGGGGCTAGATGTGGCTCCTAGTGCAAACCGGAAACCATGGCACCTTCCAGAGCCGTGGTCTCAAGGAG TCAGAGCAGGGCTGGCCCTCAGTAGCTGCAGGGAGCTTTGATGCAACTTATTTGTAAGAAGGATTTTTAAATTTTTTATGGGTAGAATTGTAGTCAGGAA AACAGAAAGGGCTTGAAATTTAATAAGTGCTGCTGGAAGGGGATTTTCCAAGCCTGGAAGGGTATTCAGCAGCTGTGGTGGGGAAACATTTCTCCTGAAA GACTGAACGTGTTTCTTCATGACAGCTGCTCAAAGCAGGTTTCTGAGATAGCTGACCGAGCTCTGGTAAATCTCTTTGTCAAATTACGAAAACTTCAGGG TGAAATCCTATGCTTCCATGTACATTACATGGCTTAAGATTAAACAAAAACATTTTTCAAGTCTCTAACTAGAGTGAACTCTAGAGCACAGTAGTTCAGA AACTATTTAGAGCTTCCAGGATATATTTCACAGCTTCAGGCATGTGATCAGTTAGAGCCGATGAAACCTATGCCCGCCTGTATATATATTAGCAGCTTAG CTAGTTCATAACCTGTATATTCTAAAGACTGCTAAGGTTTTGTTTTCATTTTAAATCCTAGCTGATTGTTGTGGTCAATGAAATACCCAGTTTCTGGAGG GCCAGGTGGGAAATGCTTTCACTGGACCAACACACAAATGATCATCCTGAGGATCTGAGCTTCCCTAGACTCCACACAATAACCTTGGGGCACCCTTTTA GAGAAGACTGTTGAAACCCACAGCACTCGTTGGGGTATGAGGAAACCAGGGCTTGGCACAGGAAGTTCCCCTTTGTAGCTAAAAGTCCAGAAAGAAAGGG TTCATCTTTTTGACTTCCAACTGATATTGGGAAGTTTGGTTGAGGTTCAAGTGTGACTCCTTCCAGAGCCACAGGTAGGGGAGTGTGAAGTTGAGGGGGA GGAAAGCTGGAAGGACTCTGCCTTGGGAGATTCCCAGCTCTGCTTTCCAGCGCTTGGTGGAATCTGGGCTGGGGAAAGACGGCACCGGGAAACTCTGCTT CCCCATTGTTTCCATCTGATCAGCTGTGGTGTGAGGACTTCTCAGACAAAGGCAAGGCCTCGTGCCCCTGCCCAGCCCATTCATGGAGCCCTGGGCCTTC TTGGCTTCCATAGATCCTAAGCTCTTGACTGTAGTTTAGCCAGACTTGTTTTGCTATCTTATAAGCAGTTCAGAATTAGGGAATGCTGGTTTTGAAGAGC AAAGGACAGGTAGTCTAGAGAGGGTCGTCTGGCCTGCTTGCTGGGTCTTTGTAACCCAGCACTTCCTCTTGCCCTCCTGGCTTTATGTTTATGGGGAGAG GACTCAATAGCTCCACCCCTTCTGGCACCAGATGGGGCTTGGTTAGTTTGCAATAAGCACCTTGCAGAGGTTAAAGCCAGCGGGTCCCTAGTCTTAGGCC CAGCCTGCTTGTGTGGGCTCTGGCCTGGCCTGGTGGCTGGCCCAGGGGGCAGCAGTGCTTAGAGCTTCTGCAGGGCTTCTCTTGTTTACACAGCTGCATC AGACAATGCCATTTCTCCCCACCACGGAACCTTCCATCTAAGATTTCTTCCAGGGAATGCCAGCAATCAGGCAGCACCCAGCTGTGGGGGCAGTGGGGTG GGGGAGACCCACATTGATGACTTTTTTTTTTTCTTTTAATGAAGAAACACCAAAGAAAGCTGTGGAAAGGACCTGCCCCACATGAAAAGGATAAGCCAAG ATGGCTGTAAACACAGAGCATTTGAGCTGCCACTCTTGGAGCACATTGATTTTTCAAAAGCCAGCTCTGTCAGGAAAGGAGGTGCTGTTATGAGCAGCTC TTCCAGTGGGCAAAGAGGACGCCCATAATTTCTTCCATTGCTAGCTCATCTGTGGGACCAATTTGGTGTAAGCAACCTGTGGCCTGCACTTGTGGCCTCG AAGGAAGCACAAACCCTCCATCCACTTCCCATTTCCTCTGCCCTTTTCCACCTCCCCCTTCCATCCCACCAGCTGCCAGTGGCTCCCAGAAAGCCTTATT GAGCCCCTTGTTGACACTTGGGGCTGCGGAGGCCTCTCCCTACTGGTCTGGCCTTTCCTGAGAGGCAGGTCTTCCGTCCTCAGAGCCTTTCTGGAACAAG GAGAATGCCTGTGCAGGTGGACACACAGGCCTGGCCTGTCGCTCTCACTTGTCTTCCAGCGGGGAGCTTCACGTTGCCGAGTGGAAGAACCATGACCTCC ACTTGCTTCCAAGGTGCTAGGGAAGTTTCAGGGTACGCTGGTTCCCCTCTCCAGCTGGAGGCCGAGTTTCTGGGGACTGCAGATTTTTCTACTCTGTGAT CGATTCAATGCCCGATGCTTCTGTTTCATTCCCGACCCTTTCTACTATGCATTTTCCTTTTATCAGGTGTATAAAGTTAAATACTGTGTATTTATCACTA AAAAGTACATGAACTTAAGAGACAACTAAGCCTTTCGTGTTTTTCCACAGGTGTTTAAGCTTCTCTGTACAGTTGAAATAAACAGACAGCAAAATGGTGC CAA

refseq_CAMKK2.F2 (forward primer)
refseq_CAMKK2.R2 (reverse primer)
Single exon skipping

Frame 0

EPGEVEGAASRDRATALQPGQHRETLSQNKQTN KQTNKQKKQRKKNGSGPGAVTHTCNPSTFGGQGG WITRSGIQD*PGQHGETPSLRKIQKLAKYGGRR L*SQLLGRLRQRTA*TWEAEVAVIRDRVTALQR GRQSETPFQKRKKKKKKRESGWSSHWLVACECPY GLEIQEKHIGTGWKSTQLTRGL*TFY*LGG*LS CQLDSLLAVWQGQ*DIPWVARPRRTVQGALQVF VSPLPRTVLLSSWGIQRTLKAPAKWLRCRMGVPQ CAG*SWRMHHVIMCL*PAQQQPGRPPG*AGGQG QQQQRKPEAL*GPAGPLILEHPPGHGVLHCGHR V*AGLCCGPRLGAGPAPGGRWPRGPP*HLRVPGP APPLRSQAVSARAVPGWAGSRWQPGHERTLHLP VPALLTRQLPAVLASAAPAADSGVSPRLHHGYA GLCAAESVYPEG*NWKGLLWCRQVGLQ*K*QYLL CNEGAVQKEADPAGRLSTSPSTPRHPASSWRLH PAQGPH*AGVPGNCHPQEAGPPQCGEAGGGPG* PQ*GPSVHGVRTGQPRARDGSAHPQTTL*RPGPF LLPGSDQRHRVLTLPEDHPP*HQTFQPPGRRRW AHQDR*LWCEQ*IQGQ*RAPLQHRGHARLHGTR VAL*DPQDLLWEGLGCLGHGCDTILLCLWPVPIH GRADHVFTQ*DQESGPGISRPARHS*GLEGPDH PYAGQEPRVEDRGAGNQAAPLGHEAWGGAVAVG G*ELHAGRSD*RGGRELSQTHSQLGNRDPGEDHD T*TLLWEPIRGQPAGGTLTVSAWKLAHQKTNQG M*VPV*AQGSKAAKTTSRAPTRPPWGRRKCSCE RQSLRGKLLGPRPRLPRTHASTAAGGGHGARVAA WIARPRMRAASPLGGCCTAFP*QHVLRKPSTCV EPRSSSLVICFFLCCFKGDKKKKRT*LHDVDRG RWLAGQAGVRSCRPKPTCILGQLLFKTFLCQKSF IVIFRTTSDIPSDCVWGLTTVERRAENSGGLRP WRWLLHLRGSRGLDVAPSANRKPWHLPEPWSQG VRAGLALSSCREL*CNLFVRRIFKFFMGRIVVRK TERA*NLISAAGRGFSKPGRVFSSCGGETFLLK D*TCFFMTAAQSRFLR*LTELW*ISLSNYENFR VKSYASMYITWLKIKQKHFSSL*LE*TLEHSSSE TI*SFQDIFHSFRHVIS*SR*NLCPPVYILAA* LVHNLYILKTAKVLFSF*ILADCCGQ*NTQFLE GQVGNAFTGPTHK*SS*GSELP*TPHNNLGAPF* RRLLKPTALVGV*GNQGLAQEVPLCS*KSRKKG FIFLTSN*YWEVWLRFKCDSFQSHR*GSVKLRG RKAGRTLPWEIPSSAFQRLVESGLGKDGTGKLCF PIVSI*SAVV*GLLRQRQGLVPLPSPFMEPWAF LASIDPKLLTVV*PDLFCYLISSSELGNAGFEE QRTGSLERVVWPACWVFVTQHFLLPSWLYVYGER TQ*LHPFWHQMGLG*FAISTLQRLKPAGP*S*A QPACVGSGLAWWLAQGAAVLRASAGLLLFTQLH QTMPFLPTTEPSI*DFFQGMPAIRQHPAVGAVGW GRPTLMTFFFSFNEETPKKAVERTCPT*KG*AK MAVNTEHLSCHSWSTLIFQKPALSGKEVLL*AA LPVGKEDAHNFFHC*LICGTNLV*ATCGLHLWPR RKHKPSIHFPFPLPFSTSPFHPTSCQWLPESLI EPLVDTWGCGGLSLLVWPFLRGRSSVLRAFLEQ GECLCRWTHRPGLSLSLVFQRGASRCRVEEP*PP LASKVLGKFQGTLVPLSSWRPSFWGLQIFLLCD RFNARCFCFIPDPFYYAFSFYQVYKVKYCVFIT KKYMNLRDN*AFRVFPQVFKLLCTVEINRQQNGA

Frame 1

SLGRSRVQRAVIVLLHSSLGNTERPCLKTNKQT NKQTNKKNKEKKMGVGRAR*LTPVIPALSEAKA GGSRGQEFKISLDNMVKPHLYEKYKN*PSMVAGA CNPSYSGD*GRELLEPGRQRLQ*SEIASLHSSV GDRARLRFRKEKKKKKKGSRGGALIGSLHVSVP TA*KYKRSTSERAGNPPS*LEGFEPFINLEVDSP VNSIPFWLFGRVSETSPGSLDPVGRFREPSRSS FLLFPAQCCYPAGGSNAHLRLQQSGSAAGWECP SVLDEAGACTMSSCVSSQPSSNRAAPQDELGGRG SSSSESQKPCEALRGLSSLSIHLGMESFIVVTE CEPGCAVDLGLARDRPLEADGQEVPLDTSGSQA RPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICP SLPYSPVSSPQSSPRLPRRPTVESHHVSITGMQ DCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTY YAMKVLSKKKLIRQAGFPRRPPPRGTRPAPGGCI QPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDD PNEDHLYMVFELVNQGPVMEVPTLKPLSEDQAR FYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDG HIKIADFGVSNEFKGSDALLSNTVGTPAFMAPE SLSETRKIFSGKALDVWAMGVTLYCFVFGQCPF MDERIMCLHSKIKSQALEFPDQPDIAEDLKDLIT RMLDKNPESRIVVPEIKLHPWVTRHGAEPLPSE DENCTLVEVTEEEVENSVKHIPSLATVILVKTM IRKRSFGNPFEGSRREERSLSAPGNLLTKKPTRE CESLSELKEARQRRQPPGHRPAPRGGGGSALVR GSPCVESCWAPAPGSPARMHPLRPEEAMEPE*L PGSLDLACAPRRLWGAAAPRFHSSMSYGNPARV* SLDRHLWLFVFSFVVLKGTKKKKGLDSMTSTVA AGWLDRRV*GVADPNPRAFWDNCFLKRFYAKNP SL*FSEPRQIYQVTVCGV*QLWKGEQKTPAV*GH GGGCCI*EGVGG*MWLLVQTGNHGTFQSRGLKE SEQGWPSVAAGSFDATYL*EGFLNFLWVEL*SG KQKGLEI**VLLEGDFPSLEGYSAAVVGKHFS*K TERVSS*QLLKAGF*DS*PSSGKSLCQITKTSG *NPMLPCTLHGLRLNKNIFQVSN*SEL*STVVQ KLFRASRIYFTASGM*SVRADETYARLYIY*QLS *FITCIF*RLLRFCFHFKS*LIVVVNEIPSFWR ARWEMLSLDQHTNDHPEDLSFPRLHTITLGHPF REDC*NPQHSLGYEETRAWHRKFPFVAKSPERKG SSF*LPTDIGKFG*GSSVTPSRATGRGV*S*GG GKLEGLCLGRFPALLSSAWWNLGWGKTAPGNSA SPLFPSDQLWCEDFSDKGKASCPCPAHSWSPGPS WLP*ILSS*L*FSQTCFAIL*AVQN*GMLVLKS KGQVV*RGSSGLLAGSL*PSTSSCPPGFMFMGR GLNSSTPSGTRWGLVSLQ*APCRG*SQRVPSLRP SLLVWALAWPGGWPRGQQCLELLQGFSCLHSCI RQCHFSPPRNLPSKISSRECQQSGSTQLWGQWG GGDPH**LFFFLLMKKHQRKLWKGPAPHEKDKPR WL*TQSI*AATLGAH*FFKSQLCQERRCCYEQL FQWAKRTPIISSIASSSVGPIWCKQPVACTCGL EGSTNPPSTSHFLCPFPPPPSIPPAASGSQKALL SPLLTLGAAEASPYWSGLS*EAGLPSSEPFWNK ENACAGGHTGLACRSHLSSSGELHVAEWKNHDL HLLPRC*GSFRVRWFPSPAGGRVSGDCRFFYSVI DSMPDASVSFPTLSTMHFPFIRCIKLNTVYLSL KST*T*ETTKPFVFFHRCLSFSVQLK*TDSKMV P

Frame 2

AWGGRGCSEP*SCYCTPAWATQRDPVSKQTNKQ TNKQTKKTKKKKWEWAGRGDSHL*SQHFRRPRR VDHEVRNSRLAWTTW*NPISTKNTKISQVWWPA PVIPATRETEAENCLNLGGRGCSDPRSRHCTPAW ATERDSVSEKKKKKKKKGVGVELSLARCM*VSL RPRNTREAHRNGLEIHPVN*RALNLLLTWRLTL LSTRFPFGCLAGSVRHPLGRSTP*DGSGSPPGLR FSSSPHSAVIQLGDPTHT*GSSKVAPLPDGSAP VCWMKLAHAPCHHVSLASPAATGPPPRMSWGAG AAAAAKARSPVRPCGASHP*ASTWAWSPSLWSPS VSRAVLWTSAWRGTGPWRPMAKRSPLTPPGPRP GPTSPVASCLCKSGPRVGWQPVAAWT*TDAASA RPCPTHPSAPRSPRLGCPGGRQWSLTTSPSRVCR TVCS*ISIP*RMKLERAPMVSSSWPTMKMTIPT MQ*RCCPKRS*SGRPAFHVALHPEAPGQLLEAA SSPGAPLSRCTRKLPSSRSWTTPMW*SWWRSWMT PMRTICTWCSNWSTKGP*WKCPPSNHSLKTRPV STSRI*SKASSTYTTRRSSTVTSNLPTSWSEKM GTSRSLTLV*AMNSRAVTRSSPTPWARPPSWHPS RSLRPARSSLGRPWMFGPWV*HYTALSLASAHS WTSGSCVYTVRSRVRPWNFQTSPT*LRT*RT*S PVCWTRTPSRGSWCRKSSCTPGSRGMGRSRCRRR MRTARWSK*LKRRSRTQSNTFPAWQP*SW*RP* YVNAPLGTHSRAAGGRNAHCQRLETCSPKNQPG NVSPCLSSRKQGSEDNLQGTDPPPVGEEEVLL*E AVPAWKVAGPPPPAPPHACIHCGRRRPWSPSSC LDRSTSHARRVASGGLLHRVSIAACPTETQHVC RASIVISGYLFFPLLF*RGQKKKKDLTP*RRPWP LAGWTGGCEELQTQTHVHFGTIAF*NVFMPKIL HCDFQNHVRYTK*LCVGFDNCGKASRKLRRSEA MEVVAAFERE*GARCGS*CKPETMAPSRAVVSRS QSRAGPQ*LQGALMQLICKKDF*IFYG*NCSQE NRKGLKFNKCCWKGIFQAWKGIQQLWWGNISPE RLNVFLHDSCSKQVSEIADRALVNLFVKLRKLQG EILCFHVHYMA*D*TKTFFKSLTRVNSRAQ*FR NYLELPGYISQLQACDQLEPMKPMPACIYISSL ASS*PVYSKDC*GFVFILNPS*LLWSMKYPVSGG PGGKCFHWTNTQMIILRI*ASLDSTQ*PWGTLL EKTVETHSTRWGMRKPGLGTGSSPL*LKVQKER VHLFDFQLILGSLVEVQV*LLPEPQVGECEVEGE ESWKDSALGDSQLCFPALGGIWAGERRHRETLL PHCFHLISCGVRTSQTKARPRAPAQPIHGALGL LGFHRS*ALDCSLARLVLLSYKQFRIRECWF*RA KDR*SREGRLACLLGLCNPALPLALLALCLWGE DSIAPPLLAPDGAWLVCNKHLAEVKASGSLVLG PACLCGLWPGLVAGPGGSSA*SFCRASLVYTAAS DNAISPHHGTFHLRFLPGNASNQAAPSCGGSGV GETHIDDFFFFF**RNTKESCGKDLPHMKRISQ DGCKHRAFELPLLEHIDFSKASSVRKGGAVMSSS SSGQRGRP*FLPLLAHLWDQFGVSNLWPALVAS KEAQTLHPLPISSALFHLPLPSHQLPVAPRKPY *APC*HLGLRRPLPTGLAFPERQVFRPQSLSGTR RMPVQVDTQAWPVALTCLPAGSFTLPSGRTMTS TCFQGAREVSGYAGSPLQLEAEFLGTADFSTL* SIQCPMLLFHSRPFLLCIFLLSGV*S*ILCIYH* KVHELKRQLSLSCFSTGV*ASLYS*NKQTAKWC Q

Sequence NM_172216

GAGCCTGGGGAGGTCGAGGGTGCAGCGAGCCGTGATCGTGCTACTGCACTCCAGCCTGGGCAACACAGAGAGACCCTGTCTCAAAACAAACAAACAAACA AACAAACAAACAAACAAAAAAAACAAAGAAAAAAAAATGGGAGTGGGCCGGGCGCGGTGACTCACACCTGTAATCCCAGCACTTTCGGAGGCCAAGGCGG GTGGATCACGAGGTCAGGAATTCAAGATTAGCCTGGACAACATGGTGAAACCCCATCTCTACGAAAAATACAAAAATTAGCCAAGTATGGTGGCCGGCGC CTGTAATCCCAGCTACTCGGGAGACTGAGGCAGAGAACTGCTTGAACCTGGGAGGCAGAGGTTGCAGTGATCCGAGATCGCGTCACTGCACTCCAGCGTG GGCGACAGAGCGAGACTCCGTTTCAGAAAAGAAAAAAAAAAAAAAAAAAAAGGGAGTCGGGGTGGAGCTCTCATTGGCTCGTTGCATGTGAGTGTCCCTA CGGCCTAGAAATACAAGAGAAGCACATCGGAACGGGCTGGAAATCCACCCAGTTAACTAGAGGGCTTTGAACCTTTTATTAACTTGGAGGTTGACTCTCC TGTCAACTCGATTCCCTTTTGGCTGTTTGGCAGGGTCAGTGAGACATCCCCTGGGTCGCTCGACCCCGTAGGACGGTTCAGGGAGCCCTCCAGGTCTTCG TTTCTCCTCTTCCCCGCACAGTGCTGTTATCCAGCTGGGGGATCCAACGCACACTTAAGGCTCCAGCAAAGTGGCTCCGCTGCCGGATGGGAGTGCCCCA GTGTGCTGGATGAAGCTGGCGCATGCACCATGTCATCATGTGTCTCTAGCCAGCCCAGCAGCAACCGGGCCGCCCCCCAGGATGAGCTGGGGGGCAGGGG CAGCAGCAGCAGCGAAAGCCAGAAGCCCTGTGAGGCCCTGCGGGGCCTCTCATCCTTGAGCATCCACCTGGGCATGGAGTCCTTCATTGTGGTCACCGAG TGTGAGCCGGGCTGTGCTGTGGACCTCGGCTTGGCGCGGGACCGGCCCCTGGAGGCCGATGGCCAAGAGGTCCCCCTTGACACCTCCGGGTCCCAGGCCC GGCCCCACCTCTCCGGTCGCAAGCTGTCTCTGCAAGAGCGGTCCCAGGGTGGGCTGGCAGCCGGTGGCAGCCTGGACATGAACGGACGCTGCATCTGCCC GTCCCTGCCCTACTCACCCGTCAGCTCCCCGCAGTCCTCGCCTCGGCTGCCCCGGCGGCCGACAGTGGAGTCTCACCACGTCTCCATCACGGGTATGCAG GACTGTGTGCAGCTGAATCAGTATACCCTGAAGGATGAAATTGGAAAGGGCTCCTATGGTGTCGTCAAGTTGGCCTACAATGAAAATGACAATACCTACT ATGCAATGAAGGTGCTGTCCAAAAAGAAGCTGATCCGGCAGGCCGGCTTTCCACGTCGCCCTCCACCCCGAGGCACCCGGCCAGCTCCTGGAGGCTGCAT CCAGCCCAGGGGCCCCATTGAGCAGGTGTACCAGGAAATTGCCATCCTCAAGAAGCTGGACCACCCCAATGTGGTGAAGCTGGTGGAGGTCCTGGATGAC CCCAATGAGGACCATCTGTACATGGTGTTCGAACTGGTCAACCAAGGGCCCGTGATGGAAGTGCCCACCCTCAAACCACTCTCTGAAGACCAGGCCCGTT TCTACTTCCAGGATCTGATCAAAGGCATCGAGTACTTACACTACCAGAAGATCATCCACCGTGACATCAAACCTTCCAACCTCCTGGTCGGAGAAGATGG GCACATCAAGATCGCTGACTTTGGTGTGAGCAATGAATTCAAGGGCAGTGACGCGCTCCTCTCCAACACCGTGGGCACGCCCGCCTTCATGGCACCCGAG TCGCTCTCTGAGACCCGCAAGATCTTCTCTGGGAAGGCCTTGGATGTTTGGGCCATGGGTGTGACACTATACTGCTTTGTCTTTGGCCAGTGCCCATTCA TGGACGAGCGGATCATGTGTTTACACAGTAAGATCAAGAGTCAGGCCCTGGAATTTCCAGACCAGCCCGACATAGCTGAGGACTTGAAGGACCTGATCAC CCGTATGCTGGACAAGAACCCCGAGTCGAGGATCGTGGTGCCGGAAATCAAGATCCTGGTGAAGACCATGATACGTAAACGCTCCTTTGGGAACCCATTC GAGGGCAGCCGGCGGGAGGAACGCTCACTGTCAGCGCCTGGAAACTTGCTCACCAAAAAACCAACCAGGGAATGTGAGTCCCTGTCTGAGCTCAAGGAAG CAAGGCAGCGAAGACAACCTCCAGGGCACCGACCCGCCCCCCGTGGGGGAGGAGGAAGTGCTCTTGTGAGAGGCAGTCCCTGCGTGGAAAGTTGCTGGGC CCCCGCCCCCGGCTCCCCCGCACGCATGCATCCACTGCGGCCGGAGGAGGCCATGGAGCCCGAGTAGCTGCCTGGATCGCTCGACCTCGCATGCGCGCCG CGTCGCCTCTGGGGGGCTGCTGCACCGCGTTTCCATAGCAGCATGTCCTACGGAAACCCAGCACGTGTGTAGAGCCTCGATCGTCATCTCTGGTTATTTG TTTTTTCCTTTGTTGTTTTAAAGGGGACAAAAAAAAAAAAAGGACTTGACTCCATGACGTCGACCGTGGCCGCTGGCTGGCTGGACAGGCGGGTGTGAGG AGTTGCAGACCCAAACCCACGTGCATTTTGGGACAATTGCTTTTTAAAACGTTTTTATGCCAAAAATCCTTCATTGTGATTTTCAGAACCACGTCAGATA TACCAAGTGACTGTGTGTGGGGTTTGACAACTGTGGAAAGGCGAGCAGAAAACTCCGGCGGTCTGAGGCCATGGAGGTGGTTGCTGCATTTGAGAGGGAG TAGGGGGCTAGATGTGGCTCCTAGTGCAAACCGGAAACCATGGCACCTTCCAGAGCCGTGGTCTCAAGGAGTCAGAGCAGGGCTGGCCCTCAGTAGCTGC AGGGAGCTTTGATGCAACTTATTTGTAAGAAGGATTTTTAAATTTTTTATGGGTAGAATTGTAGTCAGGAAAACAGAAAGGGCTTGAAATTTAATAAGTG CTGCTGGAAGGGGATTTTCCAAGCCTGGAAGGGTATTCAGCAGCTGTGGTGGGGAAACATTTCTCCTGAAAGACTGAACGTGTTTCTTCATGACAGCTGC TCAAAGCAGGTTTCTGAGATAGCTGACCGAGCTCTGGTAAATCTCTTTGTCAAATTACGAAAACTTCAGGGTGAAATCCTATGCTTCCATGTACATTACA TGGCTTAAGATTAAACAAAAACATTTTTCAAGTCTCTAACTAGAGTGAACTCTAGAGCACAGTAGTTCAGAAACTATTTAGAGCTTCCAGGATATATTTC ACAGCTTCAGGCATGTGATCAGTTAGAGCCGATGAAACCTATGCCCGCCTGTATATATATTAGCAGCTTAGCTAGTTCATAACCTGTATATTCTAAAGAC TGCTAAGGTTTTGTTTTCATTTTAAATCCTAGCTGATTGTTGTGGTCAATGAAATACCCAGTTTCTGGAGGGCCAGGTGGGAAATGCTTTCACTGGACCA ACACACAAATGATCATCCTGAGGATCTGAGCTTCCCTAGACTCCACACAATAACCTTGGGGCACCCTTTTAGAGAAGACTGTTGAAACCCACAGCACTCG TTGGGGTATGAGGAAACCAGGGCTTGGCACAGGAAGTTCCCCTTTGTAGCTAAAAGTCCAGAAAGAAAGGGTTCATCTTTTTGACTTCCAACTGATATTG GGAAGTTTGGTTGAGGTTCAAGTGTGACTCCTTCCAGAGCCACAGGTAGGGGAGTGTGAAGTTGAGGGGGAGGAAAGCTGGAAGGACTCTGCCTTGGGAG ATTCCCAGCTCTGCTTTCCAGCGCTTGGTGGAATCTGGGCTGGGGAAAGACGGCACCGGGAAACTCTGCTTCCCCATTGTTTCCATCTGATCAGCTGTGG TGTGAGGACTTCTCAGACAAAGGCAAGGCCTCGTGCCCCTGCCCAGCCCATTCATGGAGCCCTGGGCCTTCTTGGCTTCCATAGATCCTAAGCTCTTGAC TGTAGTTTAGCCAGACTTGTTTTGCTATCTTATAAGCAGTTCAGAATTAGGGAATGCTGGTTTTGAAGAGCAAAGGACAGGTAGTCTAGAGAGGGTCGTC TGGCCTGCTTGCTGGGTCTTTGTAACCCAGCACTTCCTCTTGCCCTCCTGGCTTTATGTTTATGGGGAGAGGACTCAATAGCTCCACCCCTTCTGGCACC AGATGGGGCTTGGTTAGTTTGCAATAAGCACCTTGCAGAGGTTAAAGCCAGCGGGTCCCTAGTCTTAGGCCCAGCCTGCTTGTGTGGGCTCTGGCCTGGC CTGGTGGCTGGCCCAGGGGGCAGCAGTGCTTAGAGCTTCTGCAGGGCTTCTCTTGTTTACACAGCTGCATCAGACAATGCCATTTCTCCCCACCACGGAA CCTTCCATCTAAGATTTCTTCCAGGGAATGCCAGCAATCAGGCAGCACCCAGCTGTGGGGGCAGTGGGGTGGGGGAGACCCACATTGATGACTTTTTTTT TTTCTTTTAATGAAGAAACACCAAAGAAAGCTGTGGAAAGGACCTGCCCCACATGAAAAGGATAAGCCAAGATGGCTGTAAACACAGAGCATTTGAGCTG CCACTCTTGGAGCACATTGATTTTTCAAAAGCCAGCTCTGTCAGGAAAGGAGGTGCTGTTATGAGCAGCTCTTCCAGTGGGCAAAGAGGACGCCCATAAT TTCTTCCATTGCTAGCTCATCTGTGGGACCAATTTGGTGTAAGCAACCTGTGGCCTGCACTTGTGGCCTCGAAGGAAGCACAAACCCTCCATCCACTTCC CATTTCCTCTGCCCTTTTCCACCTCCCCCTTCCATCCCACCAGCTGCCAGTGGCTCCCAGAAAGCCTTATTGAGCCCCTTGTTGACACTTGGGGCTGCGG AGGCCTCTCCCTACTGGTCTGGCCTTTCCTGAGAGGCAGGTCTTCCGTCCTCAGAGCCTTTCTGGAACAAGGAGAATGCCTGTGCAGGTGGACACACAGG CCTGGCCTGTCGCTCTCACTTGTCTTCCAGCGGGGAGCTTCACGTTGCCGAGTGGAAGAACCATGACCTCCACTTGCTTCCAAGGTGCTAGGGAAGTTTC AGGGTACGCTGGTTCCCCTCTCCAGCTGGAGGCCGAGTTTCTGGGGACTGCAGATTTTTCTACTCTGTGATCGATTCAATGCCCGATGCTTCTGTTTCAT TCCCGACCCTTTCTACTATGCATTTTCCTTTTATCAGGTGTATAAAGTTAAATACTGTGTATTTATCACTAAAAAGTACATGAACTTAAGAGACAACTAA GCCTTTCGTGTTTTTCCACAGGTGTTTAAGCTTCTCTGTACAGTTGAAATAAACAGACAGCAAAATGGTGCCAA

refseq_CAMKK2.F2 (forward primer)
refseq_CAMKK2.R2 (reverse primer)
Single exon skipping

Frame 0

EPGEVEGAASRDRATALQPGQHRETLSQNKQTN KQTNKQKKQRKKNGSGPGAVTHTCNPSTFGGQGG WITRSGIQD*PGQHGETPSLRKIQKLAKYGGRR L*SQLLGRLRQRTA*TWEAEVAVIRDRVTALQR GRQSETPFQKRKKKKKKRESGWSSHWLVACECPY GLEIQEKHIGTGWKSTQLTRGL*TFY*LGG*LS CQLDSLLAVWQGQ*DIPWVARPRRTVQGALQVF VSPLPRTVLLSSWGIQRTLKAPAKWLRCRMGVPQ CAG*SWRMHHVIMCL*PAQQQPGRPPG*AGGQG QQQQRKPEAL*GPAGPLILEHPPGHGVLHCGHR V*AGLCCGPRLGAGPAPGGRWPRGPP*HLRVPGP APPLRSQAVSARAVPGWAGSRWQPGHERTLHLP VPALLTRQLPAVLASAAPAADSGVSPRLHHGYA GLCAAESVYPEG*NWKGLLWCRQVGLQ*K*QYLL CNEGAVQKEADPAGRLSTSPSTPRHPASSWRLH PAQGPH*AGVPGNCHPQEAGPPQCGEAGGGPG* PQ*GPSVHGVRTGQPRARDGSAHPQTTL*RPGPF LLPGSDQRHRVLTLPEDHPP*HQTFQPPGRRRW AHQDR*LWCEQ*IQGQ*RAPLQHRGHARLHGTR VAL*DPQDLLWEGLGCLGHGCDTILLCLWPVPIH GRADHVFTQ*DQESGPGISRPARHS*GLEGPDH PYAGQEPRVEDRGAGNQDPGEDHDT*TLLWEPI RGQPAGGTLTVSAWKLAHQKTNQGM*VPV*AQGS KAAKTTSRAPTRPPWGRRKCSCERQSLRGKLLG PRPRLPRTHASTAAGGGHGARVAAWIARPRMRA ASPLGGCCTAFP*QHVLRKPSTCVEPRSSSLVIC FFLCCFKGDKKKKRT*LHDVDRGRWLAGQAGVR SCRPKPTCILGQLLFKTFLCQKSFIVIFRTTSD IPSDCVWGLTTVERRAENSGGLRPWRWLLHLRGS RGLDVAPSANRKPWHLPEPWSQGVRAGLALSSC REL*CNLFVRRIFKFFMGRIVVRKTERA*NLIS AAGRGFSKPGRVFSSCGGETFLLKD*TCFFMTAA QSRFLR*LTELW*ISLSNYENFRVKSYASMYIT WLKIKQKHFSSL*LE*TLEHSSSETI*SFQDIF HSFRHVIS*SR*NLCPPVYILAA*LVHNLYILKT AKVLFSF*ILADCCGQ*NTQFLEGQVGNAFTGP THK*SS*GSELP*TPHNNLGAPF*RRLLKPTAL VGV*GNQGLAQEVPLCS*KSRKKGFIFLTSN*YW EVWLRFKCDSFQSHR*GSVKLRGRKAGRTLPWE IPSSAFQRLVESGLGKDGTGKLCFPIVSI*SAV V*GLLRQRQGLVPLPSPFMEPWAFLASIDPKLLT VV*PDLFCYLISSSELGNAGFEEQRTGSLERVV WPACWVFVTQHFLLPSWLYVYGERTQ*LHPFWH QMGLG*FAISTLQRLKPAGP*S*AQPACVGSGLA WWLAQGAAVLRASAGLLLFTQLHQTMPFLPTTE PSI*DFFQGMPAIRQHPAVGAVGWGRPTLMTFF FSFNEETPKKAVERTCPT*KG*AKMAVNTEHLSC HSWSTLIFQKPALSGKEVLL*AALPVGKEDAHN FFHC*LICGTNLV*ATCGLHLWPRRKHKPSIHF PFPLPFSTSPFHPTSCQWLPESLIEPLVDTWGCG GLSLLVWPFLRGRSSVLRAFLEQGECLCRWTHR PGLSLSLVFQRGASRCRVEEP*PPLASKVLGKF QGTLVPLSSWRPSFWGLQIFLLCDRFNARCFCFI PDPFYYAFSFYQVYKVKYCVFITKKYMNLRDN* AFRVFPQVFKLLCTVEINRQQNGA

Frame 1

SLGRSRVQRAVIVLLHSSLGNTERPCLKTNKQT NKQTNKKNKEKKMGVGRAR*LTPVIPALSEAKA GGSRGQEFKISLDNMVKPHLYEKYKN*PSMVAGA CNPSYSGD*GRELLEPGRQRLQ*SEIASLHSSV GDRARLRFRKEKKKKKKGSRGGALIGSLHVSVP TA*KYKRSTSERAGNPPS*LEGFEPFINLEVDSP VNSIPFWLFGRVSETSPGSLDPVGRFREPSRSS FLLFPAQCCYPAGGSNAHLRLQQSGSAAGWECP SVLDEAGACTMSSCVSSQPSSNRAAPQDELGGRG SSSSESQKPCEALRGLSSLSIHLGMESFIVVTE CEPGCAVDLGLARDRPLEADGQEVPLDTSGSQA RPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICP SLPYSPVSSPQSSPRLPRRPTVESHHVSITGMQ DCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTY YAMKVLSKKKLIRQAGFPRRPPPRGTRPAPGGCI QPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDD PNEDHLYMVFELVNQGPVMEVPTLKPLSEDQAR FYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDG HIKIADFGVSNEFKGSDALLSNTVGTPAFMAPE SLSETRKIFSGKALDVWAMGVTLYCFVFGQCPF MDERIMCLHSKIKSQALEFPDQPDIAEDLKDLIT RMLDKNPESRIVVPEIKILVKTMIRKRSFGNPF EGSRREERSLSAPGNLLTKKPTRECESLSELKE ARQRRQPPGHRPAPRGGGGSALVRGSPCVESCWA PAPGSPARMHPLRPEEAMEPE*LPGSLDLACAP RRLWGAAAPRFHSSMSYGNPARV*SLDRHLWLF VFSFVVLKGTKKKKGLDSMTSTVAAGWLDRRV*G VADPNPRAFWDNCFLKRFYAKNPSL*FSEPRQI YQVTVCGV*QLWKGEQKTPAV*GHGGGCCI*EG VGG*MWLLVQTGNHGTFQSRGLKESEQGWPSVAA GSFDATYL*EGFLNFLWVEL*SGKQKGLEI**V LLEGDFPSLEGYSAAVVGKHFS*KTERVSS*QL LKAGF*DS*PSSGKSLCQITKTSG*NPMLPCTLH GLRLNKNIFQVSN*SEL*STVVQKLFRASRIYF TASGM*SVRADETYARLYIY*QLS*FITCIF*R LLRFCFHFKS*LIVVVNEIPSFWRARWEMLSLDQ HTNDHPEDLSFPRLHTITLGHPFREDC*NPQHS LGYEETRAWHRKFPFVAKSPERKGSSF*LPTDI GKFG*GSSVTPSRATGRGV*S*GGGKLEGLCLGR FPALLSSAWWNLGWGKTAPGNSASPLFPSDQLW CEDFSDKGKASCPCPAHSWSPGPSWLP*ILSS* L*FSQTCFAIL*AVQN*GMLVLKSKGQVV*RGSS GLLAGSL*PSTSSCPPGFMFMGRGLNSSTPSGT RWGLVSLQ*APCRG*SQRVPSLRPSLLVWALAW PGGWPRGQQCLELLQGFSCLHSCIRQCHFSPPRN LPSKISSRECQQSGSTQLWGQWGGGDPH**LFF FLLMKKHQRKLWKGPAPHEKDKPRWL*TQSI*A ATLGAH*FFKSQLCQERRCCYEQLFQWAKRTPII SSIASSSVGPIWCKQPVACTCGLEGSTNPPSTS HFLCPFPPPPSIPPAASGSQKALLSPLLTLGAA EASPYWSGLS*EAGLPSSEPFWNKENACAGGHTG LACRSHLSSSGELHVAEWKNHDLHLLPRC*GSF RVRWFPSPAGGRVSGDCRFFYSVIDSMPDASVS FPTLSTMHFPFIRCIKLNTVYLSLKST*T*ETTK PFVFFHRCLSFSVQLK*TDSKMVP

Frame 2

AWGGRGCSEP*SCYCTPAWATQRDPVSKQTNKQ TNKQTKKTKKKKWEWAGRGDSHL*SQHFRRPRR VDHEVRNSRLAWTTW*NPISTKNTKISQVWWPA PVIPATRETEAENCLNLGGRGCSDPRSRHCTPAW ATERDSVSEKKKKKKKKGVGVELSLARCM*VSL RPRNTREAHRNGLEIHPVN*RALNLLLTWRLTL LSTRFPFGCLAGSVRHPLGRSTP*DGSGSPPGLR FSSSPHSAVIQLGDPTHT*GSSKVAPLPDGSAP VCWMKLAHAPCHHVSLASPAATGPPPRMSWGAG AAAAAKARSPVRPCGASHP*ASTWAWSPSLWSPS VSRAVLWTSAWRGTGPWRPMAKRSPLTPPGPRP GPTSPVASCLCKSGPRVGWQPVAAWT*TDAASA RPCPTHPSAPRSPRLGCPGGRQWSLTTSPSRVCR TVCS*ISIP*RMKLERAPMVSSSWPTMKMTIPT MQ*RCCPKRS*SGRPAFHVALHPEAPGQLLEAA SSPGAPLSRCTRKLPSSRSWTTPMW*SWWRSWMT PMRTICTWCSNWSTKGP*WKCPPSNHSLKTRPV STSRI*SKASSTYTTRRSSTVTSNLPTSWSEKM GTSRSLTLV*AMNSRAVTRSSPTPWARPPSWHPS RSLRPARSSLGRPWMFGPWV*HYTALSLASAHS WTSGSCVYTVRSRVRPWNFQTSPT*LRT*RT*S PVCWTRTPSRGSWCRKSRSW*RP*YVNAPLGTHS RAAGGRNAHCQRLETCSPKNQPGNVSPCLSSRK QGSEDNLQGTDPPPVGEEEVLL*EAVPAWKVAG PPPPAPPHACIHCGRRRPWSPSSCLDRSTSHARR VASGGLLHRVSIAACPTETQHVCRASIVISGYL FFPLLF*RGQKKKKDLTP*RRPWPLAGWTGGCE ELQTQTHVHFGTIAF*NVFMPKILHCDFQNHVRY TK*LCVGFDNCGKASRKLRRSEAMEVVAAFERE *GARCGS*CKPETMAPSRAVVSRSQSRAGPQ*L QGALMQLICKKDF*IFYG*NCSQENRKGLKFNKC CWKGIFQAWKGIQQLWWGNISPERLNVFLHDSC SKQVSEIADRALVNLFVKLRKLQGEILCFHVHY MA*D*TKTFFKSLTRVNSRAQ*FRNYLELPGYIS QLQACDQLEPMKPMPACIYISSLASS*PVYSKD C*GFVFILNPS*LLWSMKYPVSGGPGGKCFHWT NTQMIILRI*ASLDSTQ*PWGTLLEKTVETHSTR WGMRKPGLGTGSSPL*LKVQKERVHLFDFQLIL GSLVEVQV*LLPEPQVGECEVEGEESWKDSALG DSQLCFPALGGIWAGERRHRETLLPHCFHLISCG VRTSQTKARPRAPAQPIHGALGLLGFHRS*ALD CSLARLVLLSYKQFRIRECWF*RAKDR*SREGR LACLLGLCNPALPLALLALCLWGEDSIAPPLLAP DGAWLVCNKHLAEVKASGSLVLGPACLCGLWPG LVAGPGGSSA*SFCRASLVYTAASDNAISPHHG TFHLRFLPGNASNQAAPSCGGSGVGETHIDDFFF FF**RNTKESCGKDLPHMKRISQDGCKHRAFEL PLLEHIDFSKASSVRKGGAVMSSSSSGQRGRP* FLPLLAHLWDQFGVSNLWPALVASKEAQTLHPLP ISSALFHLPLPSHQLPVAPRKPY*APC*HLGLR RPLPTGLAFPERQVFRPQSLSGTRRMPVQVDTQ AWPVALTCLPAGSFTLPSGRTMTSTCFQGAREVS GYAGSPLQLEAEFLGTADFSTL*SIQCPMLLFH SRPFLLCIFLLSGV*S*ILCIYH*KVHELKRQL SLSCFSTGV*ASLYS*NKQTAKWCQ