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Sequences

Sequence NM_206956

AATGTAGGGAAAGCAGGGCGGAGTCCTCTGCAGGCTCGGGGGAGGGGAGGGGCGTGAATGCGTGGATTTCTGTGGAGAGTGGAAACACGGGGAGTCGAGG
GGAGCATGCGCGGGCCTCAGAAAGTTTTGGGAAACCGACTCCTGGGAGCAGGGAGGAACGCGCGCTCCAGAGACAACTTCGCGGTGTGGTGAACTCTCTG
AGGAAAAACACGTGCGTGGCAACAAGTGACTGAGACCTAGAAATCCAAGCGTTGGAGGTCCTGAGGCCAGCCTAAGTCGCTTCAAAATGGAACGAAGGCG
TTTGTGGGGTTCCATTCAGAGCCGATACATCAGCATGAGTGTGTGGACAAGCCCACGGAGACTTGTGGAGCTGGCAGGGCAGAGCCTGCTGAAGGATGAG
GCCCTGGCCATTGCCGCCCTGGAGTTGCTGCCCAGGGAGCTCTTCCCGCCACTCTTCATGGCAGCCTTTGACGGGAGACACAGCCAGACCCTGAAGGCAA
TGGTGCAGGCCTGGCCCTTCACCTGCCTCCCTCTGGGAGTGCTGATGAAGGGACAACATCTTCACCTGGAGACCTTCAAAGCTGTGCTTGATGGACTTGA
TGTGCTCCTTGCCCAGGAGGTTCGCCCCAGGAGGTGGAAACTTCAAGTGCTGGATTTACGGAAGAACTCTCATCAGGACTTCTGGACTGTATGGTCTGGA
AACAGGGCCAGTCTGTACTCATTTCCAGAGCCAGAAGCAGCTCAGCCCATGACAAAGAAGCGAAAAGTAGATGGTTTGAGCACAGAGGCAGAGCAGCCCT
TCATTCCAGTAGAGGTGCTCGTAGACCTGTTCCTCAAGGAAGGTGCCTGTGATGAATTGTTCTCCTACCTCATTGAGAAAGTGAAGCGAAAGAAAAATGT
ACTACGCCTGTGCTGTAAGAAGCTGAAGATTTTTGCAATGCCCATGCAGGATATCAAGATGATCCTGAAAATGGTGCAGCTGGACTCTATTGAAGATTTG
GAAGTGACTTGTACCTGGAAGCTACCCACCTTGGCGAAATTTTCTCCTTACCTGGGCCAGATGATTAATCTGCGTAGACTCCTCCTCTCCCACATCCATG
CATCTTCCTACATTTCCCCGGAGAAGGAAGAGCAGTATATCGCCCAGTTCACCTCTCAGTTCCTCAGTCTGCAGTGCCTGCAGGCTCTCTATGTGGACTC
TTTATTTTTCCTTAGAGGCCGCCTGGATCAGTTGCTCAGGCACGTGATGAACCCCTTGGAAACCCTCTCAATAACTAACTGCCGGCTTTCGGAAGGGGAT
GTGATGCATCTGTCCCAGAGTCCCAGCGTCAGTCAGCTAAGTGTCCTGAGTCTAAGTGGGGTCATGCTGACCGATGTAAGTCCCGAGCCCCTCCAAGCTC
TGCTGGAGAGAGCCTCTGCCACCCTCCAGGACCTGGTCTTTGATGAGTGTGGGATCACGGATGATCAGCTCCTTGCCCTCCTGCCTTCCCTGAGCCACTG
CTCCCAGCTTACAACCTTAAGCTTCTACGGGAATTCCATCTCCATATCTGCCTTGCAGAGTCTCCTGCAGCACCTCATCGGGCTGAGCAATCTGACCCAC
GTGCTGTATCCTGTCCCCCTGGAGAGTTATGAGGACATCCATGGTACCCTCCACCTGGAGAGGCTTGCCTATCTGCATGCCAGGCTCAGGGAGTTGCTGT
GTGAGTTGGGGCGGCCCAGCATGGTCTGGCTTAGTGCCAACCCCTGTCCTCACTGTGGGGACAGAACCTTCTATGACCCGGAGCCCATCCTGTGCCCCTG
TTTCATGCCTAACTAGCTGGGTGCACATATCAAATGCTTCATTCTGCATACTTGGACACTAAAGCCAGGATGTGCATGCATCTTGAAGCAACAAAGCAGC
CACAGTTTCAGACAAATGTTCAGTGTGAGTGAGGAAAACATGTTCAGTGAGGAAAAAACATTCAGACAAATGTTCAGTGAGGAAAAAAAGGGGAAGTTGG
GGATAGGCAGATGTTGACTTGAGGAGTTAATGTGATCTTTGGGGAGATACATCTTATAGAGTTAGAAATAGAATCTGAATTTCTAAAGGGAGATTCTGGC
TTGGGAAGTACATGTAGGAGTTAATCCCTGTGTAGACTGTTGTAAAGAAACTGTTGAAAATAAAGAGAAGCAATGTGAAGCA
  • refseq_PRAME.F1 (forward primer)
  • refseq_PRAME.R1 (reverse primer)
  • Alternative acceptor

Frame 0

NVGKAGRSPLQARGRGGA*MRGFLWRVETRGVE
GSMRGPQKVLGNRLLGAGRNARSRDNFAVW*TL*
GKTRAWQQVTET*KSKRWRS*GQPKSLQNGTKA
FVGFHSEPIHQHECVDKPTETCGAGRAEPAEG*
GPGHCRPGVAAQGALPATLHGSL*RETQPDPEGN
GAGLALHLPPSGSADEGTTSSPGDLQSCA*WT*
CAPCPGGSPQEVETSSAGFTEELSSGLLDCMVW
KQGQSVLISRARSSSAHDKEAKSRWFEHRGRAAL
HSSRGARRPVPQGRCL**IVLLPH*ESEAKEKC
TTPVL*EAEDFCNAHAGYQDDPENGAAGLY*RF
GSDLYLEATHLGEIFSLPGPDD*SA*TPPLPHPC
IFLHFPGEGRAVYRPVHLSVPQSAVPAGSLCGL
FIFP*RPPGSVAQARDEPLGNPLNN*LPAFGRG
CDASVPESQRQSAKCPESKWGHADRCKSRAPPSS
AGESLCHPPGPGL**VWDHG*SAPCPPAFPEPL
LPAYNLKLLREFHLHICLAESPAAPHRAEQSDP
RAVSCPPGEL*GHPWYPPPGEACLSACQAQGVAV
*VGAAQHGLA*CQPLSSLWGQNLL*PGAHPVPL
FHA*LAGCTYQMLHSAYLDTKARMCMHLEATKQ
PQFQTNVQCE*GKHVQ*GKNIQTNVQ*GKKGEVG
DRQMLT*GVNVIFGEIHLIELEIESEFLKGDSG
LGSTCRS*SLCRLL*RNC*K*REAM*S

Frame 1

M*GKQGGVLCRLGGGEGRECVDFCGEWKHGESR
GACAGLRKFWETDSWEQGGTRAPETTSRCGELS
EEKHVRGNK*LRPRNPSVGGPEASLSRFKMERRR
LWGSIQSRYISMSVWTSPRRLVELAGQSLLKDE
ALAIAALELLPRELFPPLFMAAFDGRHSQTLKA
MVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLD
VLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSG
NRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQP
FIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNV
LRLCCKKLKIFAMPMQDIKMILKMVQLDSIEDL
EVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIH
ASSYISPEKEEQYIAQFTSQFLSLQCLQALYVDS
LFFLRGRLDQLLRHVMNPLETLSITNCRLSEGD
VMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQA
LLERASATLQDLVFDECGITDDQLLALLPSLSHC
SQLTTLSFYGNSISISALQSLLQHLIGLSNLTH
VLYPVPLESYEDIHGTLHLERLAYLHARLRELL
CELGRPSMVWLSANPCPHCGDRTFYDPEPILCPC
FMPN*LGAHIKCFILHTWTLKPGCACILKQQSS
HSFRQMFSVSEENMFSEEKTFRQMFSEEKKGKL
GIGRC*LEELM*SLGRYIL*S*K*NLNF*REILA
WEVHVGVNPCVDCCKETVENKEKQCEA

Frame 2

CRESRAESSAGSGEGRGVNAWISVESGNTGSRG
EHARASESFGKPTPGSREERALQRQLRGVVNSL
RKNTCVATSD*DLEIQALEVLRPA*VASKWNEG
VCGVPFRADTSA*VCGQAHGDLWSWQGRAC*RMR
PWPLPPWSCCPGSSSRHSSWQPLTGDTARP*RQ
WCRPGPSPASLWEC**RDNIFTWRPSKLCLMDL
MCSLPRRFAPGGGNFKCWIYGRTLIRTSGLYGLE
TGPVCTHFQSQKQLSP*QRSEK*MV*AQRQSSP
SFQ*RCS*TCSSRKVPVMNCSPTSLRK*SERKM
YYACAVRS*RFLQCPCRISR*S*KWCSWTLLKIW
K*LVPGSYPPWRNFLLTWAR*LICVDSSSPTSM
HLPTFPRRRKSSISPSSPLSSSVCSACRLSMWT
LYFSLEAAWISCSGT**TPWKPSQ*LTAGFRKGM
*CICPRVPASVS*VS*V*VGSC*PM*VPSPSKL
CWREPLPPSRTWSLMSVGSRMISSLPSCLP*AT
APSLQP*ASTGIPSPYLPCRVSCSTSSG*AI*PT
CCILSPWRVMRTSMVPSTWRGLPICMPGSGSCC
VSWGGPAWSGLVPTPVLTVGTEPSMTRSPSCAP
VSCLTSWVHISNASFCILGH*SQDVHAS*SNKAA
TVSDKCSV*VRKTCSVRKKHSDKCSVRKKRGSW
G*ADVDLRS*CDLWGDTSYRVRNRI*ISKGRFW
LGKYM*ELIPV*TVVKKLLKIKRSNVK

Sequence NM_206955

AATGTAGGGAAAGCAGGGCGGAGTCCTCTGCAGGCTCGGGGGAGGGGAGGGGCGTGAATGCGTGGATTTCTGTGGAGAGTGGAAACACGGGGAGTCGAGG
GGAGCATGCGCGGGCCTCAGAAAGTTTTGGGAAACCGACTCCTGGGAGCAGGGAGGAACGCGCGCTCCAGAGACAACTTCGCGGTGTGGTGAACTCTCTG
AGGAAAAACCATTTTGATTATTACTCTCAGACGTGCGTGGCAACAAGTGACTGAGACCTAGAAATCCAAGCGTTGGAGGTCCTGAGGCCAGCCTAAGTCG
CTTCAAAATGGAACGAAGGCGTTTGTGGGGTTCCATTCAGAGCCGATACATCAGCATGAGTGTGTGGACAAGCCCACGGAGACTTGTGGAGCTGGCAGGG
CAGAGCCTGCTGAAGGATGAGGCCCTGGCCATTGCCGCCCTGGAGTTGCTGCCCAGGGAGCTCTTCCCGCCACTCTTCATGGCAGCCTTTGACGGGAGAC
ACAGCCAGACCCTGAAGGCAATGGTGCAGGCCTGGCCCTTCACCTGCCTCCCTCTGGGAGTGCTGATGAAGGGACAACATCTTCACCTGGAGACCTTCAA
AGCTGTGCTTGATGGACTTGATGTGCTCCTTGCCCAGGAGGTTCGCCCCAGGAGGTGGAAACTTCAAGTGCTGGATTTACGGAAGAACTCTCATCAGGAC
TTCTGGACTGTATGGTCTGGAAACAGGGCCAGTCTGTACTCATTTCCAGAGCCAGAAGCAGCTCAGCCCATGACAAAGAAGCGAAAAGTAGATGGTTTGA
GCACAGAGGCAGAGCAGCCCTTCATTCCAGTAGAGGTGCTCGTAGACCTGTTCCTCAAGGAAGGTGCCTGTGATGAATTGTTCTCCTACCTCATTGAGAA
AGTGAAGCGAAAGAAAAATGTACTACGCCTGTGCTGTAAGAAGCTGAAGATTTTTGCAATGCCCATGCAGGATATCAAGATGATCCTGAAAATGGTGCAG
CTGGACTCTATTGAAGATTTGGAAGTGACTTGTACCTGGAAGCTACCCACCTTGGCGAAATTTTCTCCTTACCTGGGCCAGATGATTAATCTGCGTAGAC
TCCTCCTCTCCCACATCCATGCATCTTCCTACATTTCCCCGGAGAAGGAAGAGCAGTATATCGCCCAGTTCACCTCTCAGTTCCTCAGTCTGCAGTGCCT
GCAGGCTCTCTATGTGGACTCTTTATTTTTCCTTAGAGGCCGCCTGGATCAGTTGCTCAGGCACGTGATGAACCCCTTGGAAACCCTCTCAATAACTAAC
TGCCGGCTTTCGGAAGGGGATGTGATGCATCTGTCCCAGAGTCCCAGCGTCAGTCAGCTAAGTGTCCTGAGTCTAAGTGGGGTCATGCTGACCGATGTAA
GTCCCGAGCCCCTCCAAGCTCTGCTGGAGAGAGCCTCTGCCACCCTCCAGGACCTGGTCTTTGATGAGTGTGGGATCACGGATGATCAGCTCCTTGCCCT
CCTGCCTTCCCTGAGCCACTGCTCCCAGCTTACAACCTTAAGCTTCTACGGGAATTCCATCTCCATATCTGCCTTGCAGAGTCTCCTGCAGCACCTCATC
GGGCTGAGCAATCTGACCCACGTGCTGTATCCTGTCCCCCTGGAGAGTTATGAGGACATCCATGGTACCCTCCACCTGGAGAGGCTTGCCTATCTGCATG
CCAGGCTCAGGGAGTTGCTGTGTGAGTTGGGGCGGCCCAGCATGGTCTGGCTTAGTGCCAACCCCTGTCCTCACTGTGGGGACAGAACCTTCTATGACCC
GGAGCCCATCCTGTGCCCCTGTTTCATGCCTAACTAGCTGGGTGCACATATCAAATGCTTCATTCTGCATACTTGGACACTAAAGCCAGGATGTGCATGC
ATCTTGAAGCAACAAAGCAGCCACAGTTTCAGACAAATGTTCAGTGTGAGTGAGGAAAACATGTTCAGTGAGGAAAAAACATTCAGACAAATGTTCAGTG
AGGAAAAAAAGGGGAAGTTGGGGATAGGCAGATGTTGACTTGAGGAGTTAATGTGATCTTTGGGGAGATACATCTTATAGAGTTAGAAATAGAATCTGAA
TTTCTAAAGGGAGATTCTGGCTTGGGAAGTACATGTAGGAGTTAATCCCTGTGTAGACTGTTGTAAAGAAACTGTTGAAAATAAAGAGAAGCAATGTGAA
GCA
  • refseq_PRAME.F1 (forward primer)
  • refseq_PRAME.R1 (reverse primer)
  • Alternative acceptor

Frame 0

NVGKAGRSPLQARGRGGA*MRGFLWRVETRGVE
GSMRGPQKVLGNRLLGAGRNARSRDNFAVW*TL*
GKTILIITLRRAWQQVTET*KSKRWRS*GQPKS
LQNGTKAFVGFHSEPIHQHECVDKPTETCGAGR
AEPAEG*GPGHCRPGVAAQGALPATLHGSL*RET
QPDPEGNGAGLALHLPPSGSADEGTTSSPGDLQ
SCA*WT*CAPCPGGSPQEVETSSAGFTEELSSG
LLDCMVWKQGQSVLISRARSSSAHDKEAKSRWFE
HRGRAALHSSRGARRPVPQGRCL**IVLLPH*E
SEAKEKCTTPVL*EAEDFCNAHAGYQDDPENGA
AGLY*RFGSDLYLEATHLGEIFSLPGPDD*SA*T
PPLPHPCIFLHFPGEGRAVYRPVHLSVPQSAVP
AGSLCGLFIFP*RPPGSVAQARDEPLGNPLNN*
LPAFGRGCDASVPESQRQSAKCPESKWGHADRCK
SRAPPSSAGESLCHPPGPGL**VWDHG*SAPCP
PAFPEPLLPAYNLKLLREFHLHICLAESPAAPH
RAEQSDPRAVSCPPGEL*GHPWYPPPGEACLSAC
QAQGVAV*VGAAQHGLA*CQPLSSLWGQNLL*P
GAHPVPLFHA*LAGCTYQMLHSAYLDTKARMCM
HLEATKQPQFQTNVQCE*GKHVQ*GKNIQTNVQ*
GKKGEVGDRQMLT*GVNVIFGEIHLIELEIESE
FLKGDSGLGSTCRS*SLCRLL*RNC*K*REAM*
S

Frame 1

M*GKQGGVLCRLGGGEGRECVDFCGEWKHGESR
GACAGLRKFWETDSWEQGGTRAPETTSRCGELS
EEKPF*LLLSDVRGNK*LRPRNPSVGGPEASLSR
FKMERRRLWGSIQSRYISMSVWTSPRRLVELAG
QSLLKDEALAIAALELLPRELFPPLFMAAFDGR
HSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFK
AVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQD
FWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGL
STEAEQPFIPVEVLVDLFLKEGACDELFSYLIEK
VKRKKNVLRLCCKKLKIFAMPMQDIKMILKMVQ
LDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRR
LLLSHIHASSYISPEKEEQYIAQFTSQFLSLQCL
QALYVDSLFFLRGRLDQLLRHVMNPLETLSITN
CRLSEGDVMHLSQSPSVSQLSVLSLSGVMLTDV
SPEPLQALLERASATLQDLVFDECGITDDQLLAL
LPSLSHCSQLTTLSFYGNSISISALQSLLQHLI
GLSNLTHVLYPVPLESYEDIHGTLHLERLAYLH
ARLRELLCELGRPSMVWLSANPCPHCGDRTFYDP
EPILCPCFMPN*LGAHIKCFILHTWTLKPGCAC
ILKQQSSHSFRQMFSVSEENMFSEEKTFRQMFS
EEKKGKLGIGRC*LEELM*SLGRYIL*S*K*NLN
F*REILAWEVHVGVNPCVDCCKETVENKEKQCE
A

Frame 2

CRESRAESSAGSGEGRGVNAWISVESGNTGSRG
EHARASESFGKPTPGSREERALQRQLRGVVNSL
RKNHFDYYSQTCVATSD*DLEIQALEVLRPA*V
ASKWNEGVCGVPFRADTSA*VCGQAHGDLWSWQG
RAC*RMRPWPLPPWSCCPGSSSRHSSWQPLTGD
TARP*RQWCRPGPSPASLWEC**RDNIFTWRPS
KLCLMDLMCSLPRRFAPGGGNFKCWIYGRTLIRT
SGLYGLETGPVCTHFQSQKQLSP*QRSEK*MV*
AQRQSSPSFQ*RCS*TCSSRKVPVMNCSPTSLR
K*SERKMYYACAVRS*RFLQCPCRISR*S*KWCS
WTLLKIWK*LVPGSYPPWRNFLLTWAR*LICVD
SSSPTSMHLPTFPRRRKSSISPSSPLSSSVCSA
CRLSMWTLYFSLEAAWISCSGT**TPWKPSQ*LT
AGFRKGM*CICPRVPASVS*VS*V*VGSC*PM*
VPSPSKLCWREPLPPSRTWSLMSVGSRMISSLP
SCLP*ATAPSLQP*ASTGIPSPYLPCRVSCSTSS
G*AI*PTCCILSPWRVMRTSMVPSTWRGLPICM
PGSGSCCVSWGGPAWSGLVPTPVLTVGTEPSMT
RSPSCAPVSCLTSWVHISNASFCILGH*SQDVHA
S*SNKAATVSDKCSV*VRKTCSVRKKHSDKCSV
RKKRGSWG*ADVDLRS*CDLWGDTSYRVRNRI*
ISKGRFWLGKYM*ELIPV*TVVKKLLKIKRSNVK

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