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Sequences

Sequence NM_021056

GGTGCGTCCTGGTCCACCATGGCCAAACCAACAAGCAAAGATTCAGGCTTGAAGGAGAAGTTTAAGATTCTGTTGGGACTGGGAACACCGAGGCCAAATC CCAGGTCTGCAGAGGGTAAACAGACGGAGTTTATCATCACCGCGGAAATACTGAGAGAACTGAGCATGGAATGTGGCCTCAACAATCGCATCCGGATGAT AGGGCAGATTTGTGAAGTCGCAAAAACCAAGAAATTTGAAGAGCACGCAGTGGAAGCACTCTGGAAGGCGGTCGCGGATCTGTTGCAGCCGGAGCGGCCG CTGGAGGCCCGGCACGCGGTGCTGGCTCTGCTGAAGGCCATCGTGCAGGGGCAGGGCGAGCGTTTGGGGGTCCTCAGAGCCCTCTTCTTTAAGGTCATCA AGGATTACCCTTCCAACGAAGACCTTCACGAAAGGCTGGAGGTTTTCAAGGCCCTCACAGACAATGGGAGACACATCACCTACTTGGAGGAAGAGCTGGC TGACTTTGTCCTGCAGTGGATGGATGTTGGCTTGTCCTCGGAATTCCTTCTGGTGCTGGTGAACTTGGTCAAATTCAATAGCTGTTACCTCGACGAGTAC ATCGCAAGGATGGTTCAGATGATCTGTCTGCTGTGCGTCCGGACCGCGTCCTCTGTGGACATAGAGGTCTCCCTGCAGGTGCTGGACGCCGTGGTCTGCT ACAACTGCCTGCCGGCTGAGAGCCTCCCGCTGTTCATCGTTACCCTCTGTCGCACCATCAACGTCAAGGAGCTCTGCGAGCCTTGCTGGAAGCTGATGCG GAACCTCCTTGGCACCCACCTGGGCCACAGCGCCATCTACAACATGTGCCACCTCATGGAGGACAGAGCCTACATGGAGGACGCGCCCCTGCTGAGAGGA GCCGTGTTTTTTGTGGGCATGGCTCTCTGGGGAGCCCACCGGCTCTATTCTCTCAGGAACTCGCCGACATCTGTGTTGCCATCATTTTACCAGGCCATGG CATGTCCGAACGAGGTGGTGTCCTATGAGATCGTCCTGTCCATCACCAGGCTCATCAAGAAGTATAGGAAGGAGCTCCAGGTGGTGGCGTGGGACATTCT GCTGAACATCATCGAACGGCTCCTTCAGCAGCTCCAGACCTTGGACAGCCCGGAGCTCAGGACCATCGTCCATGACCTGTTGACCACGGTGGAGGAGCTG TGTGACCAGAACGAGTTCCACGGGTCTCAGGAGAGATACTTTGAACTGGTGGAGAGATGTGCGGACCAGAGGCCTGAGTCCTCCCTCCTGAACCTGATCT CCTATAGAGCGCAGTCCATCCACCCGGCCAAGGACGGCTGGATTCAGAACCTGCAGGCGCTGATGGAGAGATTCTTCAGGAGCGAGTCCCGAGGCGCCGT GCGCATCAAGGTGCTGGACGTGCTGTCCTTTGTGCTGCTCATCAACAGGCAGTTCTATGAGGAGGAGCTGATTAACTCAGTGGTCATCTCGCAGCTCTCC CACATCCCCGAGGATAAAGACCACCAGGTCCGAAAGCTGGCCACCCAGTTGCTGGTGGACCTGGCAGAGGGCTGCCACACACACCACTTCAACAGCCTGC TGGACATCATCGAGAAGGTGATGGCCCGCTCCCTCTCCCCACCCCCGGAGCTGGAAGAAAGGGATGTGGCCGCATACTCGGCCTCCTTGGAGGATGTGAA GACAGCCGTCCTGGGGCTTCTGGTCATCCTTCAGACCAAGCTGTACACCCTGCCTGCAAGCCACGCCACGCGTGTGTATGAGATGCTGGTCAGCCACATT CAGCTCCACTACAAGCACAGCTACACCCTGCCAATCGCGAGCAGCATCCGGCTGCAGGCCTTTGACTTCCTGTTGCTGCTGCGGGCCGACTCACTGCACC GCCTGGGCCTGCCCAACAAGGATGGAGTCGTGCGGTTCAGCCCCTACTGCGTCTGCGACTACATGGAGCCAGAGAGAGGCTCTGAGAAGAAGACCAGCGG CCCCCTTTCTCCTCCCACAGGGCCTCCTGGCCCGGCGCCTGCAGGCCCCGCCGTGCGGCTGGGGTCCGTGCCCTACTCCCTGCTCTTCCGCGTCCTGCTG CAGTGCTTGAAGCAGGAGTCTGACTGGAAGGTGCTGAAGCTGGTTCTGGGCAGGCTGCCTGAGTCCCTGCGCTATAAAGTGCTCATCTTTACTTCCCCTT GCAGTGTGGACCAGCTGTGCTCTGCTCTCTGCTCCATGCTTTCAGGCCCAAAGACACTGGAGCGGCTCCGAGGCGCCCCAGAAGGCTTCTCCAGAACTGA CTTGCACCTGGCCGTGGTTCCAGTGCTGACAGCATTAATCTCTTACCATAACTACCTGGACAAAACCAAACAGCGCGAGATGGTCTACTGCCTGGAGCAG GGCCTCATCCACCGCTGTGCCAGCCAGTGCGTCGTGGCCTTGTCCATCTGCAGCGTGGAGATGCCTGACATCATCATCAAGGCGCTGCCTGTTCTGGTGG TGAAGCTCACGCACATCTCAGCCACAGCCAGCATGGCCGTCCCACTGCTGGAGTTCCTGTCCACTCTGGCCAGGCTGCCGCACCTCTACAGGAACTTTGC CGCGGAGCAGTATGCCAGTGTGTTCGCCATCTCCCTGCCGTACACCAACCCCTCCAAGTTTAATCAGTACATCGTGTGTCTGGCCCATCACGTCATAGCC ATGTGGTTCATCAGGTGCCGCCTGCCCTTCCGGAAGGATTTTGTCCCTTTCATCACTAAGGGCCTGCGGTCCAATGTCCTCTTGTCTTTTGATGACACCC CCGAGAAGGACAGCTTCAGGGCCCGGAGTACTAGTCTCAACGAGAGACCCAAGAGGATACAGACGTCCCTCACCAGTGCCAGCTTGGGGTCTGCAGATGA GAACTCCGTGGCCCAGGCTGACGATAGCCTGAAAAACCTCCACCTGGAGCTCACGGAAACCTGTCTGGACATGATGGCTCGATACGTCTTCTCCAACTTC ACGGCTGTCCCGAAGAGGTCTCCTGTGGGCGAGTTCCTCCTAGCGGGTGGCAGGACCAAAACCTGGCTGGTTGGGAACAAGCTTGTCACTGTGACGACAA GCGTGGGAACCGGGACCCGGTCGTTACTAGGCCTGGACTCGGGGGAGCTGCAGTCCGGCCCGGAGTCGAGCTCCAGCCCCGGGGTGCATGTGAGACAGAC CAAGGAGGCGCCGGCCAAGCTGGAGTCCCAGGCTGGGCAGCAGGTGTCCCGTGGGGCCCGGGATCGGGTCCGTTCCATGTCGGGGGGCCATGGTCTTCGA GTTGGCGCCCTGGACGTGCCGGCCTCCCAGTTCCTGGGCAGTGCCACTTCTCCAGGACCACGGACTGCACCAGCCGCGAAACCTGAGAAGGCCTCAGCTG GCACCCGGGTTCCTGTGCAGGAGAAGACGAACCTGGCGGCCTATGTGCCCCTGCTGACCCAGGGCTGGGCGGAGATCCTGGTCCGGAGGCCCACAGGGAA CACCAGCTGGCTGATGAGCCTGGAGAACCCGCTCAGCCCTTTCTCCTCGGACATCAACAACATGCCCCTGCAGGAGCTGTCTAACGCCCTCATGGCGGCT GAGCGCTTCAAGGAGCACCGGGACACAGCCCTGTACAAGTCACTGTCGGTGCCGGCAGCCAGCACGGCCAAACCCCCTCCTCTGCCTCGCTCCAACACAG TGGCCTCTTTCTCCTCCCTGTACCAGTCCAGCTGCCAAGGACAGCTGCACAGGAGCGTTTCCTGGGCAGACTCCGCCGTGGTCATGGAGGAGGGAAGTCC GGGCGAGGTTCCTGTGCTGGTGGAGCCCCCAGGGTTGGAGGACGTTGAGGCAGCGCTAGGCATGGACAGGCGCACGGATGCCTACAGCAGGTCGTCCTCA GTCTCCAGCCAGGAGGAGAAGTCGCTCCACGCGGAGGAGCTGGTTGGCAGGGGCATCCCCATCGAGCGAGTCGTCTCCTCGGAGGGTGGCCGGCCCTCTG TGGACCTCTCCTTCCAGCCCTCGCAGCCCCTGAGCAAGTCCAGCTCCTCTCCCGAGCTGCAGACTCTGCAGGACATCCTCGGGGACCCTGGGGACAAGGC CGACGTGGGCCGGCTGAGCCCTGAGGTTAAGGCCCGGTCACAGTCAGGGACCCTGGACGGGGAAAGTGCTGCCTGGTCGGCCTCGGGCGAAGACAGTCGG GGCCAGCCCGAGGGTCCCTTGCCTTCCAGCTCCCCCCGCTCGCCCAGTGGCCTCCGGCCCCGAGGTTACACCATCTCCGACTCGGCCCCATCACGCAGGG GCAAGAGAGTAGAGAGGGACGCCTTAAAGAGCAGAGCCACAGCCTCCAATGCAGAGAAAGTGCCAGGCATCAACCCCAGTTTCGTGTTCCTGCAGCTCTA CCATTCCCCCTTCTTTGGCGACGAGTCAAACAAGCCAATCCTGCTGCCCAATGAGTCACAGTCCTTTGAGCGGTCGGTGCAGCTCCTCGACCAGATCCCA TCATACGACACCCACAAGATCGCCGTCCTGTATGTTGGAGAAGGCCAGAGCAACAGCGAGCTCGCCATCCTGTCCAATGAGCATGGCTCCTACAGGTACA CGGAGTTCCTGACGGGCCTGGGCCGGCTCATCGAGCTGAAGGACTGCCAGCCGGACAAGGTGTACCTGGGAGGCCTGGACGTGTGTGGTGAGGACGGCCA GTTCACCTACTGCTGGCACGATGACATCATGCAAGCCGTCTTCCACATCGCCACCCTGATGCCCACCAAGGACGTGGACAAGCACCGCTGCGACAAGAAG CGCCACCTGGGCAACGACTTTGTGTCCATTGTCTACAATGACTCCGGTGAGGACTTCAAGCTTGGCACCATCAAGGGCCAGTTCAACTTTGTCCACGTGA TCGTCACCCCGCTGGACTACGAGTGCAACCTGGTGTCCCTGCAGTGCAGGAAAGACATGGAGGGCCTTGTGGACACCAGCGTGGCCAAGATCGTGTCTGA CCGCAACCTGCCCTTCGTGGCCCGCCAGATGGCCCTGCACGCAAATATGGCCTCACAGGTGCATCATAGCCGCTCCAACCCCACCGATATCTACCCCTCC AAGTGGATTGCCCGGCTCCGCCACATCAAGCGGCTCCGCCAGCGGATCTGCGAGGAAGCCGCCTACTCCAACCCCAGCCTACCTCTGGTGCACCCTCCGT CCCATAGCAAAGCCCCTGCACAGACTCCAGCCGAGCCCACACCTGGCTATGAGGTGGGCCAGCGGAAGCGCCTCATCTCCTCGGTGGAGGACTTCACCGA GTTTGTGTGAGGCCGGGGCCCTCCCTCCTGCACTGGCCTTGGACGGTATTGCCTGTCAGTGAAATAAATAAAGTCCTGACCCCAGTGCACAGACATAGAG GCACAGATTGC

refseq_TSC2.F2 (forward primer)
refseq_TSC2.R2 (reverse primer)
Alternative acceptor

Frame 0

GASWSTMAKPTSKDSGLKEKFKILLGLGTPRPN PRSAEGKQTEFIITAEILRELSMECGLNNRIRMI GQICEVAKTKKFEEHAVEALWKAVADLLQPERP LEARHAVLALLKAIVQGQGERLGVLRALFFKVI KDYPSNEDLHERLEVFKALTDNGRHITYLEEELA DFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEY IARMVQMICLLCVRTASSVDIEVSLQVLDAVVC YNCLPAESLPLFIVTLCRTINVKELCEPCWKLMR NLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRG AVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAM ACPNEVVSYEIVLSITRLIKKYRKELQVVAWDIL LNIIERLLQQLQTLDSPELRTIVHDLLTTVEEL CDQNEFHGSQERYFELVERCADQRPESSLLNLI SYRAQSIHPAKDGWIQNLQALMERFFRSESRGAV RIKVLDVLSFVLLINRQFYEEELINSVVISQLS HIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSL LDIIEKVMARSLSPPPELEERDVAAYSASLEDVK TAVLGLLVILQTKLYTLPASHATRVYEMLVSHI QLHYKHSYTLPIASSIRLQAFDFLLLLRADSLH RLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSG PLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLL QCLKQESDWKVLKLVLGRLPESLRYKVLIFTSP CSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTD LHLAVVPVLTALISYHNYLDKTKQREMVYCLEQ GLIHRCASQCVVALSICSVEMPDIIIKALPVLV VKLTHISATASMAVPLLEFLSTLARLPHLYRNFA AEQYASVFAISLPYTNPSKFNQYIVCLAHHVIA MWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDT PEKDSFRARSTSLNERPKRIQTSLTSASLGSADE NSVAQADDSLKNLHLELTETCLDMMARYVFSNF TAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTT SVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQT KEAPAKLESQAGQQVSRGARDRVRSMSGGHGLR VGALDVPASQFLGSATSPGPRTAPAAKPEKASA GTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGN TSWLMSLENPLSPFSSDINNMPLQELSNALMAA ERFKEHRDTALYKSLSVPAASTAKPPPLPRSNT VASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSP GEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSS VSSQEEKSLHAEELVGRGIPIERVVSSEGGRPS VDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKA DVGRLSPEVKARSQSGTLDGESAAWSASGEDSR GQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRR GKRVERDALKSRATASNAEKVPGINPSFVFLQLY HSPFFGDESNKPILLPNESQSFERSVQLLDQIP SYDTHKIAVLYVGEGQSNSELAILSNEHGSYRY TEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQ FTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKK RHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHV IVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSD RNLPFVARQMALHANMASQVHHSRSNPTDIYPS KWIARLRHIKRLRQRICEEAAYSNPSLPLVHPP SHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTE FV*GRGPPSCTGLGRYCLSVK*IKS*PQCTDIE AQI

Frame 1

VRPGPPWPNQQAKIQA*RRSLRFCWDWEHRGQI PGLQRVNRRSLSSPRKY*EN*AWNVASTIASG* *GRFVKSQKPRNLKSTQWKHSGRRSRICCSRSGR WRPGTRCWLC*RPSCRGRASVWGSSEPSSLRSS RITLPTKTFTKGWRFSRPSQTMGDTSPTWRKSW LTLSCSGWMLACPRNSFWCW*TWSNSIAVTSTST SQGWFR*SVCCASGPRPLWT*RSPCRCWTPWSA TTACRLRASRCSSLPSVAPSTSRSSASLAGS*C GTSLAPTWATAPSTTCATSWRTEPTWRTRPC*EE PCFLWAWLSGEPTGSILSGTRRHLCCHHFTRPW HVRTRWCPMRSSCPSPGSSRSIGRSSRWWRGTF C*TSSNGSFSSSRPWTARSSGPSSMTC*PRWRSC VTRTSSTGLRRDTLNWWRDVRTRGLSPPS*T*S PIERSPSTRPRTAGFRTCRR*WRDSSGASPEAP CASRCWTCCPLCCSSTGSSMRRS*LTQWSSRSSP TSPRIKTTRSESWPPSCWWTWQRAATHTTSTAC WTSSRR*WPAPSPHPRSWKKGMWPHTRPPWRM* RQPSWGFWSSFRPSCTPCLQATPRVCMRCWSATF SSTTSTATPCQSRAASGCRPLTSCCCCGPTHCT AWACPTRMESCGSAPTASATTWSQREALRRRPA APFLLPQGLLARRLQAPPCGWGPCPTPCSSASCC SA*SRSLTGRC*SWFWAGCLSPCAIKCSSLLPL AVWTSCALLSAPCFQAQRHWSGSEAPQKASPEL TCTWPWFQC*QH*SLTITTWTKPNSARWSTAWSR ASSTAVPASASWPCPSAAWRCLTSSSRRCLFWW *SSRTSQPQPAWPSHCWSSCPLWPGCRTSTGTL PRSSMPVCSPSPCRTPTPPSLISTSCVWPITS*P CGSSGAACPSGRILSLSSLRACGPMSSCLLMTP PRRTASGPGVLVSTRDPRGYRRPSPVPAWGLQM RTPWPRLTIA*KTSTWSSRKPVWT*WLDTSSPTS RLSRRGLLWASSS*RVAGPKPGWLGTSLSL*RQ AWEPGPGRY*AWTRGSCSPARSRAPAPGCM*DR PRRRRPSWSPRLGSRCPVGPGIGSVPCRGAMVFE LAPWTCRPPSSWAVPLLQDHGLHQPRNLRRPQL APGFLCRRRRTWRPMCPC*PRAGRRSWSGGPQG TPAG**AWRTRSALSPRTSTTCPCRSCLTPSWRL SASRSTGTQPCTSHCRCRQPARPNPLLCLAPTQ WPLSPPCTSPAAKDSCTGAFPGQTPPWSWRREV RARFLCWWSPQGWRTLRQR*AWTGARMPTAGRPQ SPARRRSRSTRRSWLAGASPSSESSPRRVAGPL WTSPSSPRSP*ASPAPLPSCRLCRTSSGTLGTR PTWAG*ALRLRPGHSQGPWTGKVLPGRPRAKTVG ASPRVPCLPAPPARPVASGPEVTPSPTRPHHAG ARE*RGTP*RAEPQPPMQRKCQASTPVSCSCSS TIPPSLATSQTSQSCCPMSHSPLSGRCSSSTRSH HTTPTRSPSCMLEKARATASSPSCPMSMAPTGT RSS*RAWAGSSS*RTASRTRCTWEAWTCVVRTA SSPTAGTMTSCKPSSTSPP*CPPRTWTSTAATRS ATWATTLCPLSTMTPVRTSSLAPSRASSTLST* SSPRWTTSATWCPCSAGKTWRALWTPAWPRSCL TATCPSWPARWPCTQIWPHRCIIAAPTPPISTPP SGLPGSATSSGSASGSARKPPTPTPAYLWCTLR PIAKPLHRLQPSPHLAMRWASGSASSPRWRTSP SLCEAGALPPALALDGIACQ*NK*SPDPSAQT*R HRL

Frame 2

CVLVHHGQTNKQRFRLEGEV*DSVGTGNTEAKS QVCRG*TDGVYHHRGNTERTEHGMWPQQSHPDD RADL*SRKNQEI*RARSGSTLEGGRGSVAAGAA AGGPARGAGSAEGHRAGAGRAFGGPQSPLL*GHQ GLPFQRRPSRKAGGFQGPHRQWETHHLLGGRAG *LCPAVDGCWLVLGIPSGAGELGQIQ*LLPRRV HRKDGSDDLSAVRPDRVLCGHRGLPAGAGRRGLL QLPAG*EPPAVHRYPLSHHQRQGALRALLEADA EPPWHPPGPQRHLQHVPPHGGQSLHGGRAPAER SRVFCGHGSLGSPPALFSQELADICVAIILPGHG MSERGGVL*DRPVHHQAHQEV*EGAPGGGVGHS AEHHRTAPSAAPDLGQPGAQDHRP*PVDHGGGA V*PERVPRVSGEIL*TGGEMCGPEA*VLPPEPDL L*SAVHPPGQGRLDSEPAGADGEILQERVPRRR AHQGAGRAVLCAAHQQAVL*GGAD*LSGHLAAL PHPRG*RPPGPKAGHPVAGGPGRGLPHTPLQQPA GHHREGDGPLPLPTPGAGRKGCGRILGLLGGCE DSRPGASGHPSDQAVHPACKPRHACV*DAGQPH SAPLQAQLHPANREQHPAAGL*LPVAAAGRLTAP PGPAQQGWSRAVQPLLRLRLHGARERL*EEDQR PPFSSHRASWPGACRPRRAAGVRALLPALPRPA AVLEAGV*LEGAEAGSGQAA*VPAL*SAHLYFPL QCGPAVLCSLLHAFRPKDTGAAPRRPRRLLQN* LAPGRGSSADSINLLP*LPGQNQTARDGLLPGA GPHPPLCQPVRRGLVHLQRGDA*HHHQGAACSGG EAHAHLSHSQHGRPTAGVPVHSGQAAAPLQELC RGAVCQCVRHLPAVHQPLQV*SVHRVSGPSRHS HVVHQVPPALPEGFCPFHH*GPAVQCPLVF**HP REGQLQGPEY*SQRETQEDTDVPHQCQLGVCR* ELRGPG*R*PEKPPPGAHGNLSGHDGSIRLLQL HGCPEEVSCGRVPPSGWQDQNLAGWEQACHCDDK RGNRDPVVTRPGLGGAAVRPGVELQPRGACETD QGGAGQAGVPGWAAGVPWGPGSGPFHVGGPWSS SWRPGRAGLPVPGQCHFSRTTDCTSRET*EGLSW HPGSCAGEDEPGGLCAPADPGLGGDPGPEAHRE HQLADEPGEPAQPFLLGHQQHAPAGAV*RPHGG *ALQGAPGHSPVQVTVGAGSQHGQTPSSASLQHS GLFLLPVPVQLPRTAAQERFLGRLRRGHGGGKS GRGSCAGGAPRVGGR*GSARHGQAHGCLQQVVL SLQPGGEVAPRGGAGWQGHPHRASRLLGGWPALC GPLLPALAAPEQVQLLSRAADSAGHPRGPWGQG RRGPAEP*G*GPVTVRDPGRGKCCLVGLGRRQS GPARGSLAFQLPPLAQWPPAPRLHHLRLGPITQG QESREGRLKEQSHSLQCRESARHQPQFRVPAAL PFPLLWRRVKQANPAAQ*VTVL*AVGAAPRPDP IIRHPQDRRPVCWRRPEQQRARHPVQ*AWLLQVH GVPDGPGPAHRAEGLPAGQGVPGRPGRVW*GRP VHLLLAR*HHASRLPHRHPDAHQGRGQAPLRQE APPGQRLCVHCLQ*LR*GLQAWHHQGPVQLCPRD RHPAGLRVQPGVPAVQERHGGPCGHQRGQDRV* PQPALRGPPDGPARKYGLTGAS*PLQPHRYLPL QVDCPAPPHQAAPPADLRGSRLLQPQPTSGAPSV P*QSPCTDSSRAHTWL*GGPAEAPHLLGGGLHR VCVRPGPSLLHWPWTVLPVSEINKVLTPVHRHR GTDC

Sequence NM_021055

GGTGCGTCCTGGTCCACCATGGCCAAACCAACAAGCAAAGATTCAGGCTTGAAGGAGAAGTTTAAGATTCTGTTGGGACTGGGAACACCGAGGCCAAATC CCAGGTCTGCAGAGGGTAAACAGACGGAGTTTATCATCACCGCGGAAATACTGAGAGAACTGAGCATGGAATGTGGCCTCAACAATCGCATCCGGATGAT AGGGCAGATTTGTGAAGTCGCAAAAACCAAGAAATTTGAAGAGCACGCAGTGGAAGCACTCTGGAAGGCGGTCGCGGATCTGTTGCAGCCGGAGCGGCCG CTGGAGGCCCGGCACGCGGTGCTGGCTCTGCTGAAGGCCATCGTGCAGGGGCAGGGCGAGCGTTTGGGGGTCCTCAGAGCCCTCTTCTTTAAGGTCATCA AGGATTACCCTTCCAACGAAGACCTTCACGAAAGGCTGGAGGTTTTCAAGGCCCTCACAGACAATGGGAGACACATCACCTACTTGGAGGAAGAGCTGGC TGACTTTGTCCTGCAGTGGATGGATGTTGGCTTGTCCTCGGAATTCCTTCTGGTGCTGGTGAACTTGGTCAAATTCAATAGCTGTTACCTCGACGAGTAC ATCGCAAGGATGGTTCAGATGATCTGTCTGCTGTGCGTCCGGACCGCGTCCTCTGTGGACATAGAGGTCTCCCTGCAGGTGCTGGACGCCGTGGTCTGCT ACAACTGCCTGCCGGCTGAGAGCCTCCCGCTGTTCATCGTTACCCTCTGTCGCACCATCAACGTCAAGGAGCTCTGCGAGCCTTGCTGGAAGCTGATGCG GAACCTCCTTGGCACCCACCTGGGCCACAGCGCCATCTACAACATGTGCCACCTCATGGAGGACAGAGCCTACATGGAGGACGCGCCCCTGCTGAGAGGA GCCGTGTTTTTTGTGGGCATGGCTCTCTGGGGAGCCCACCGGCTCTATTCTCTCAGGAACTCGCCGACATCTGTGTTGCCATCATTTTACCAGGCCATGG CATGTCCGAACGAGGTGGTGTCCTATGAGATCGTCCTGTCCATCACCAGGCTCATCAAGAAGTATAGGAAGGAGCTCCAGGTGGTGGCGTGGGACATTCT GCTGAACATCATCGAACGGCTCCTTCAGCAGCTCCAGACCTTGGACAGCCCGGAGCTCAGGACCATCGTCCATGACCTGTTGACCACGGTGGAGGAGCTG TGTGACCAGAACGAGTTCCACGGGTCTCAGGAGAGATACTTTGAACTGGTGGAGAGATGTGCGGACCAGAGGCCTGAGTCCTCCCTCCTGAACCTGATCT CCTATAGAGCGCAGTCCATCCACCCGGCCAAGGACGGCTGGATTCAGAACCTGCAGGCGCTGATGGAGAGATTCTTCAGGAGCGAGTCCCGAGGCGCCGT GCGCATCAAGGTGCTGGACGTGCTGTCCTTTGTGCTGCTCATCAACAGGCAGTTCTATGAGGAGGAGCTGATTAACTCAGTGGTCATCTCGCAGCTCTCC CACATCCCCGAGGATAAAGACCACCAGGTCCGAAAGCTGGCCACCCAGTTGCTGGTGGACCTGGCAGAGGGCTGCCACACACACCACTTCAACAGCCTGC TGGACATCATCGAGAAGGTGATGGCCCGCTCCCTCTCCCCACCCCCGGAGCTGGAAGAAAGGGATGTGGCCGCATACTCGGCCTCCTTGGAGGATGTGAA GACAGCCGTCCTGGGGCTTCTGGTCATCCTTCAGACCAAGCTGTACACCCTGCCTGCAAGCCACGCCACGCGTGTGTATGAGATGCTGGTCAGCCACATT CAGCTCCACTACAAGCACAGCTACACCCTGCCAATCGCGAGCAGCATCCGGCTGCAGGCCTTTGACTTCCTGTTGCTGCTGCGGGCCGACTCACTGCACC GCCTGGGCCTGCCCAACAAGGATGGAGTCGTGCGGTTCAGCCCCTACTGCGTCTGCGACTACATGGAGCCAGAGAGAGGCTCTGAGAAGAAGACCAGCGG CCCCCTTTCTCCTCCCACAGGGCCTCCTGGCCCGGCGCCTGCAGGCCCCGCCGTGCGGCTGGGGTCCGTGCCCTACTCCCTGCTCTTCCGCGTCCTGCTG CAGTGCTTGAAGCAGGAGTCTGACTGGAAGGTGCTGAAGCTGGTTCTGGGCAGGCTGCCTGAGTCCCTGCGCTATAAAGTGCTCATCTTTACTTCCCCTT GCAGTGTGGACCAGCTGTGCTCTGCTCTCTGCTCCATGCTTTCAGGCCCAAAGACACTGGAGCGGCTCCGAGGCGCCCCAGAAGGCTTCTCCAGAACTGA CTTGCACCTGGCCGTGGTTCCAGTGCTGACAGCATTAATCTCTTACCATAACTACCTGGACAAAACCAAACAGCGCGAGATGGTCTACTGCCTGGAGCAG GGCCTCATCCACCGCTGTGCCAGCCAGTGCGTCGTGGCCTTGTCCATCTGCAGCGTGGAGATGCCTGACATCATCATCAAGGCGCTGCCTGTTCTGGTGG TGAAGCTCACGCACATCTCAGCCACAGCCAGCATGGCCGTCCCACTGCTGGAGTTCCTGTCCACTCTGGCCAGGCTGCCGCACCTCTACAGGAACTTTGC CGCGGAGCAGTATGCCAGTGTGTTCGCCATCTCCCTGCCGTACACCAACCCCTCCAAGTTTAATCAGTACATCGTGTGTCTGGCCCATCACGTCATAGCC ATGTGGTTCATCAGGTGCCGCCTGCCCTTCCGGAAGGATTTTGTCCCTTTCATCACTAAGGGCCTGCGGTCCAATGTCCTCTTGTCTTTTGATGACACCC CCGAGAAGGACAGCTTCAGGGCCCGGAGTACTAGTCTCAACGAGAGACCCAAGAGCAGGATACAGACGTCCCTCACCAGTGCCAGCTTGGGGTCTGCAGA TGAGAACTCCGTGGCCCAGGCTGACGATAGCCTGAAAAACCTCCACCTGGAGCTCACGGAAACCTGTCTGGACATGATGGCTCGATACGTCTTCTCCAAC TTCACGGCTGTCCCGAAGAGGTCTCCTGTGGGCGAGTTCCTCCTAGCGGGTGGCAGGACCAAAACCTGGCTGGTTGGGAACAAGCTTGTCACTGTGACGA CAAGCGTGGGAACCGGGACCCGGTCGTTACTAGGCCTGGACTCGGGGGAGCTGCAGTCCGGCCCGGAGTCGAGCTCCAGCCCCGGGGTGCATGTGAGACA GACCAAGGAGGCGCCGGCCAAGCTGGAGTCCCAGGCTGGGCAGCAGGTGTCCCGTGGGGCCCGGGATCGGGTCCGTTCCATGTCGGGGGGCCATGGTCTT CGAGTTGGCGCCCTGGACGTGCCGGCCTCCCAGTTCCTGGGCAGTGCCACTTCTCCAGGACCACGGACTGCACCAGCCGCGAAACCTGAGAAGGCCTCAG CTGGCACCCGGGTTCCTGTGCAGGAGAAGACGAACCTGGCGGCCTATGTGCCCCTGCTGACCCAGGGCTGGGCGGAGATCCTGGTCCGGAGGCCCACAGG GAACACCAGCTGGCTGATGAGCCTGGAGAACCCGCTCAGCCCTTTCTCCTCGGACATCAACAACATGCCCCTGCAGGAGCTGTCTAACGCCCTCATGGCG GCTGAGCGCTTCAAGGAGCACCGGGACACAGCCCTGTACAAGTCACTGTCGGTGCCGGCAGCCAGCACGGCCAAACCCCCTCCTCTGCCTCGCTCCAACA CAGTGGCCTCTTTCTCCTCCCTGTACCAGTCCAGCTGCCAAGGACAGCTGCACAGGAGCGTTTCCTGGGCAGACTCCGCCGTGGTCATGGAGGAGGGAAG TCCGGGCGAGGTTCCTGTGCTGGTGGAGCCCCCAGGGTTGGAGGACGTTGAGGCAGCGCTAGGCATGGACAGGCGCACGGATGCCTACAGCAGGTCGTCC TCAGTCTCCAGCCAGGAGGAGAAGTCGCTCCACGCGGAGGAGCTGGTTGGCAGGGGCATCCCCATCGAGCGAGTCGTCTCCTCGGAGGGTGGCCGGCCCT CTGTGGACCTCTCCTTCCAGCCCTCGCAGCCCCTGAGCAAGTCCAGCTCCTCTCCCGAGCTGCAGACTCTGCAGGACATCCTCGGGGACCCTGGGGACAA GGCCGACGTGGGCCGGCTGAGCCCTGAGGTTAAGGCCCGGTCACAGTCAGGGACCCTGGACGGGGAAAGTGCTGCCTGGTCGGCCTCGGGCGAAGACAGT CGGGGCCAGCCCGAGGGTCCCTTGCCTTCCAGCTCCCCCCGCTCGCCCAGTGGCCTCCGGCCCCGAGGTTACACCATCTCCGACTCGGCCCCATCACGCA GGGGCAAGAGAGTAGAGAGGGACGCCTTAAAGAGCAGAGCCACAGCCTCCAATGCAGAGAAAGTGCCAGGCATCAACCCCAGTTTCGTGTTCCTGCAGCT CTACCATTCCCCCTTCTTTGGCGACGAGTCAAACAAGCCAATCCTGCTGCCCAATGAGTCACAGTCCTTTGAGCGGTCGGTGCAGCTCCTCGACCAGATC CCATCATACGACACCCACAAGATCGCCGTCCTGTATGTTGGAGAAGGCCAGAGCAACAGCGAGCTCGCCATCCTGTCCAATGAGCATGGCTCCTACAGGT ACACGGAGTTCCTGACGGGCCTGGGCCGGCTCATCGAGCTGAAGGACTGCCAGCCGGACAAGGTGTACCTGGGAGGCCTGGACGTGTGTGGTGAGGACGG CCAGTTCACCTACTGCTGGCACGATGACATCATGCAAGCCGTCTTCCACATCGCCACCCTGATGCCCACCAAGGACGTGGACAAGCACCGCTGCGACAAG AAGCGCCACCTGGGCAACGACTTTGTGTCCATTGTCTACAATGACTCCGGTGAGGACTTCAAGCTTGGCACCATCAAGGGCCAGTTCAACTTTGTCCACG TGATCGTCACCCCGCTGGACTACGAGTGCAACCTGGTGTCCCTGCAGTGCAGGAAAGACATGGAGGGCCTTGTGGACACCAGCGTGGCCAAGATCGTGTC TGACCGCAACCTGCCCTTCGTGGCCCGCCAGATGGCCCTGCACGCAAATATGGCCTCACAGGTGCATCATAGCCGCTCCAACCCCACCGATATCTACCCC TCCAAGTGGATTGCCCGGCTCCGCCACATCAAGCGGCTCCGCCAGCGGATCTGCGAGGAAGCCGCCTACTCCAACCCCAGCCTACCTCTGGTGCACCCTC CGTCCCATAGCAAAGCCCCTGCACAGACTCCAGCCGAGCCCACACCTGGCTATGAGGTGGGCCAGCGGAAGCGCCTCATCTCCTCGGTGGAGGACTTCAC CGAGTTTGTGTGAGGCCGGGGCCCTCCCTCCTGCACTGGCCTTGGACGGTATTGCCTGTCAGTGAAATAAATAAAGTCCTGACCCCAGTGCACAGACATA GAGGCACAGATTGC

refseq_TSC2.F2 (forward primer)
refseq_TSC2.R2 (reverse primer)
Alternative acceptor

Frame 0

GASWSTMAKPTSKDSGLKEKFKILLGLGTPRPN PRSAEGKQTEFIITAEILRELSMECGLNNRIRMI GQICEVAKTKKFEEHAVEALWKAVADLLQPERP LEARHAVLALLKAIVQGQGERLGVLRALFFKVI KDYPSNEDLHERLEVFKALTDNGRHITYLEEELA DFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEY IARMVQMICLLCVRTASSVDIEVSLQVLDAVVC YNCLPAESLPLFIVTLCRTINVKELCEPCWKLMR NLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRG AVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAM ACPNEVVSYEIVLSITRLIKKYRKELQVVAWDIL LNIIERLLQQLQTLDSPELRTIVHDLLTTVEEL CDQNEFHGSQERYFELVERCADQRPESSLLNLI SYRAQSIHPAKDGWIQNLQALMERFFRSESRGAV RIKVLDVLSFVLLINRQFYEEELINSVVISQLS HIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSL LDIIEKVMARSLSPPPELEERDVAAYSASLEDVK TAVLGLLVILQTKLYTLPASHATRVYEMLVSHI QLHYKHSYTLPIASSIRLQAFDFLLLLRADSLH RLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSG PLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLL QCLKQESDWKVLKLVLGRLPESLRYKVLIFTSP CSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTD LHLAVVPVLTALISYHNYLDKTKQREMVYCLEQ GLIHRCASQCVVALSICSVEMPDIIIKALPVLV VKLTHISATASMAVPLLEFLSTLARLPHLYRNFA AEQYASVFAISLPYTNPSKFNQYIVCLAHHVIA MWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDT PEKDSFRARSTSLNERPKSRIQTSLTSASLGSAD ENSVAQADDSLKNLHLELTETCLDMMARYVFSN FTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVT TSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQ TKEAPAKLESQAGQQVSRGARDRVRSMSGGHGL RVGALDVPASQFLGSATSPGPRTAPAAKPEKAS AGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTG NTSWLMSLENPLSPFSSDINNMPLQELSNALMA AERFKEHRDTALYKSLSVPAASTAKPPPLPRSN TVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGS PGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSS SVSSQEEKSLHAEELVGRGIPIERVVSSEGGRP SVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDK ADVGRLSPEVKARSQSGTLDGESAAWSASGEDS RGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSR RGKRVERDALKSRATASNAEKVPGINPSFVFLQL YHSPFFGDESNKPILLPNESQSFERSVQLLDQI PSYDTHKIAVLYVGEGQSNSELAILSNEHGSYR YTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDG QFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDK KRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVH VIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVS DRNLPFVARQMALHANMASQVHHSRSNPTDIYP SKWIARLRHIKRLRQRICEEAAYSNPSLPLVHP PSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFT EFV*GRGPPSCTGLGRYCLSVK*IKS*PQCTDI EAQI

Frame 1

VRPGPPWPNQQAKIQA*RRSLRFCWDWEHRGQI PGLQRVNRRSLSSPRKY*EN*AWNVASTIASG* *GRFVKSQKPRNLKSTQWKHSGRRSRICCSRSGR WRPGTRCWLC*RPSCRGRASVWGSSEPSSLRSS RITLPTKTFTKGWRFSRPSQTMGDTSPTWRKSW LTLSCSGWMLACPRNSFWCW*TWSNSIAVTSTST SQGWFR*SVCCASGPRPLWT*RSPCRCWTPWSA TTACRLRASRCSSLPSVAPSTSRSSASLAGS*C GTSLAPTWATAPSTTCATSWRTEPTWRTRPC*EE PCFLWAWLSGEPTGSILSGTRRHLCCHHFTRPW HVRTRWCPMRSSCPSPGSSRSIGRSSRWWRGTF C*TSSNGSFSSSRPWTARSSGPSSMTC*PRWRSC VTRTSSTGLRRDTLNWWRDVRTRGLSPPS*T*S PIERSPSTRPRTAGFRTCRR*WRDSSGASPEAP CASRCWTCCPLCCSSTGSSMRRS*LTQWSSRSSP TSPRIKTTRSESWPPSCWWTWQRAATHTTSTAC WTSSRR*WPAPSPHPRSWKKGMWPHTRPPWRM* RQPSWGFWSSFRPSCTPCLQATPRVCMRCWSATF SSTTSTATPCQSRAASGCRPLTSCCCCGPTHCT AWACPTRMESCGSAPTASATTWSQREALRRRPA APFLLPQGLLARRLQAPPCGWGPCPTPCSSASCC SA*SRSLTGRC*SWFWAGCLSPCAIKCSSLLPL AVWTSCALLSAPCFQAQRHWSGSEAPQKASPEL TCTWPWFQC*QH*SLTITTWTKPNSARWSTAWSR ASSTAVPASASWPCPSAAWRCLTSSSRRCLFWW *SSRTSQPQPAWPSHCWSSCPLWPGCRTSTGTL PRSSMPVCSPSPCRTPTPPSLISTSCVWPITS*P CGSSGAACPSGRILSLSSLRACGPMSSCLLMTP PRRTASGPGVLVSTRDPRAGYRRPSPVPAWGLQ MRTPWPRLTIA*KTSTWSSRKPVWT*WLDTSSPT SRLSRRGLLWASSS*RVAGPKPGWLGTSLSL*R QAWEPGPGRY*AWTRGSCSPARSRAPAPGCM*D RPRRRRPSWSPRLGSRCPVGPGIGSVPCRGAMVF ELAPWTCRPPSSWAVPLLQDHGLHQPRNLRRPQ LAPGFLCRRRRTWRPMCPC*PRAGRRSWSGGPQ GTPAG**AWRTRSALSPRTSTTCPCRSCLTPSWR LSASRSTGTQPCTSHCRCRQPARPNPLLCLAPT QWPLSPPCTSPAAKDSCTGAFPGQTPPWSWRRE VRARFLCWWSPQGWRTLRQR*AWTGARMPTAGRP QSPARRRSRSTRRSWLAGASPSSESSPRRVAGP LWTSPSSPRSP*ASPAPLPSCRLCRTSSGTLGT RPTWAG*ALRLRPGHSQGPWTGKVLPGRPRAKTV GASPRVPCLPAPPARPVASGPEVTPSPTRPHHA GARE*RGTP*RAEPQPPMQRKCQASTPVSCSCS STIPPSLATSQTSQSCCPMSHSPLSGRCSSSTRS HHTTPTRSPSCMLEKARATASSPSCPMSMAPTG TRSS*RAWAGSSS*RTASRTRCTWEAWTCVVRT ASSPTAGTMTSCKPSSTSPP*CPPRTWTSTAATR SATWATTLCPLSTMTPVRTSSLAPSRASSTLST *SSPRWTTSATWCPCSAGKTWRALWTPAWPRSC LTATCPSWPARWPCTQIWPHRCIIAAPTPPISTP PSGLPGSATSSGSASGSARKPPTPTPAYLWCTL RPIAKPLHRLQPSPHLAMRWASGSASSPRWRTS PSLCEAGALPPALALDGIACQ*NK*SPDPSAQT* RHRL

Frame 2

CVLVHHGQTNKQRFRLEGEV*DSVGTGNTEAKS QVCRG*TDGVYHHRGNTERTEHGMWPQQSHPDD RADL*SRKNQEI*RARSGSTLEGGRGSVAAGAA AGGPARGAGSAEGHRAGAGRAFGGPQSPLL*GHQ GLPFQRRPSRKAGGFQGPHRQWETHHLLGGRAG *LCPAVDGCWLVLGIPSGAGELGQIQ*LLPRRV HRKDGSDDLSAVRPDRVLCGHRGLPAGAGRRGLL QLPAG*EPPAVHRYPLSHHQRQGALRALLEADA EPPWHPPGPQRHLQHVPPHGGQSLHGGRAPAER SRVFCGHGSLGSPPALFSQELADICVAIILPGHG MSERGGVL*DRPVHHQAHQEV*EGAPGGGVGHS AEHHRTAPSAAPDLGQPGAQDHRP*PVDHGGGA V*PERVPRVSGEIL*TGGEMCGPEA*VLPPEPDL L*SAVHPPGQGRLDSEPAGADGEILQERVPRRR AHQGAGRAVLCAAHQQAVL*GGAD*LSGHLAAL PHPRG*RPPGPKAGHPVAGGPGRGLPHTPLQQPA GHHREGDGPLPLPTPGAGRKGCGRILGLLGGCE DSRPGASGHPSDQAVHPACKPRHACV*DAGQPH SAPLQAQLHPANREQHPAAGL*LPVAAAGRLTAP PGPAQQGWSRAVQPLLRLRLHGARERL*EEDQR PPFSSHRASWPGACRPRRAAGVRALLPALPRPA AVLEAGV*LEGAEAGSGQAA*VPAL*SAHLYFPL QCGPAVLCSLLHAFRPKDTGAAPRRPRRLLQN* LAPGRGSSADSINLLP*LPGQNQTARDGLLPGA GPHPPLCQPVRRGLVHLQRGDA*HHHQGAACSGG EAHAHLSHSQHGRPTAGVPVHSGQAAAPLQELC RGAVCQCVRHLPAVHQPLQV*SVHRVSGPSRHS HVVHQVPPALPEGFCPFHH*GPAVQCPLVF**HP REGQLQGPEY*SQRETQEQDTDVPHQCQLGVCR *ELRGPG*R*PEKPPPGAHGNLSGHDGSIRLLQ LHGCPEEVSCGRVPPSGWQDQNLAGWEQACHCDD KRGNRDPVVTRPGLGGAAVRPGVELQPRGACET DQGGAGQAGVPGWAAGVPWGPGSGPFHVGGPWS SSWRPGRAGLPVPGQCHFSRTTDCTSRET*EGLS WHPGSCAGEDEPGGLCAPADPGLGGDPGPEAHR EHQLADEPGEPAQPFLLGHQQHAPAGAV*RPHG G*ALQGAPGHSPVQVTVGAGSQHGQTPSSASLQH SGLFLLPVPVQLPRTAAQERFLGRLRRGHGGGK SGRGSCAGGAPRVGGR*GSARHGQAHGCLQQVV LSLQPGGEVAPRGGAGWQGHPHRASRLLGGWPAL CGPLLPALAAPEQVQLLSRAADSAGHPRGPWGQ GRRGPAEP*G*GPVTVRDPGRGKCCLVGLGRRQ SGPARGSLAFQLPPLAQWPPAPRLHHLRLGPITQ GQESREGRLKEQSHSLQCRESARHQPQFRVPAA LPFPLLWRRVKQANPAAQ*VTVL*AVGAAPRPD PIIRHPQDRRPVCWRRPEQQRARHPVQ*AWLLQV HGVPDGPGPAHRAEGLPAGQGVPGRPGRVW*GR PVHLLLAR*HHASRLPHRHPDAHQGRGQAPLRQ EAPPGQRLCVHCLQ*LR*GLQAWHHQGPVQLCPR DRHPAGLRVQPGVPAVQERHGGPCGHQRGQDRV *PQPALRGPPDGPARKYGLTGAS*PLQPHRYLP LQVDCPAPPHQAAPPADLRGSRLLQPQPTSGAPS VP*QSPCTDSSRAHTWL*GGPAEAPHLLGGGLH RVCVRPGPSLLHWPWTVLPVSEINKVLTPVHRH RGTDC