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Sequence NM_198708
CCTGCTGGGAGGCGAAGACCAGGCGGGCACTGTTGCCCGGCGACGCTCCGGCGCTGGGTCCCCGAGGCCCGGCCCCTCCCCGGGAGGAGGTGGGCTTCGAGTCACGTGACCCGTGCCCTACGGGAGGGGGTGCGGTCGGGGACCCGGCAGGAGGCGGCCGAGAAGAGAGGACCGTGGGGGCGTTCGCGTGGCTCCCAGCC
CGGGACCCCACCCCCGCTGGACAGTGGGGGAAACTGAGGCCTGAGCGGGCCCACACAGGACCATGAAGGTGCTTCTCCTCACAGGGCTGGGGGCCCTGTT
CTTCGCCTATTATTGGGATGACAACTTCGACCCAGCCAGCCTCCAGGGAGCGCGAGTGCTGCTGACAGGGGCCAACGCTGGTGTTGGTGAGGAGCTGGCC
TATCACTACGCGCGTCTGGGCTCCCACCTGGTGCTCACTGCCCACACTGAGGCTCTCCTGCAGAAGGCGGGCTGGACTACCTCGTGCTGAACCACATCGG
CGGCGCCCCGGCCGGCACGCGAGCCCGCAGCCCCCAGGCAACTCGCTGGCTCATGCAGGTAAACTTTGTGAGCTACGTGCAACTGACGTCGCGGGCGCTG
CCCAGCCTGACGGACAGCAAGGGCTCCCTGGTGGTGGTGTCCTCGCTGCTCGGCCGCGTGCCCACGTCGTTCTCCACTCCCTACTCGGCGGCCAAGTTTG
CGCTGGACGGCTTCTTCGGCTCCCTGCGGCGGGAGCTGGACGTGCAGGACGTGAACGTGGCCATCACCATGTGCGTCCTGGGCCTCCGAGATCGCGCCTC
CGCCGCCGAGGCAGTCAGGGGAGTCACGAGGGTCAAGGCGGCCCCGGGGCCCAAGGCAGCCCTGGCCGTGATCCGCGGCGGCGCCACGCGCGCGGCCGGC
GTCTTCTACCCGTGGCGTTTCCGCCTGCTGTGCTTGCTCCGGCGCTGGCTACCGCGCCCGCGGGCCTGGTTTATCCGCCAGGAGCTCAACGTCACGGCCG
CGGCAGCCTGAGCACCGGGGGGTGCCCCTCCAGTCCCAGACGGCAATGTTCCTCCCTCCAACTGTCCCTGGAGCCAGAACACTCACAGAGACACCCCTGA
GAGGGTGGCCACAGCCCAAGATGAAGTCATCAAGACAGAAAAGCAAAACCGAGAAAAACGACGGGCACCTGGAACCAGTCACGGCTTGGGAGGTGCAGGT
GCCCCGTGTTAGGCGCCTTTGTCGGGGACTTGCAAGGCCTCACCTGTTTGGCCATGATTGATGACGTGACTGCTTCCATTTTGCAGATGAGGAAACTAAG
GCTCAGAGAGGCCACGCCACCCTTGAGCCACCCATGGACCCCTCTCCATCTCCTGCCTGCGCCTTTAAGTCCCTGATTTATTCTTTCCATTCATTCCATC
TGGGAGGAACCCCCCCAACTCCTGCCAGCTTCCCCTAGCTGGGGTCTCTGGTACTCTTCACACCTGCAGGGGCGTCTACACTGTTCGTCTACCTGGTGGC
AGGGTCTGAGCGGGAGGAGGAGGGAAAGAGTGTGTTCTGAGCTGGACCCAGCCTCTTGTTCGAGAATAAAAACTCTTCTTCTCTTGCA
- refseq_HSD11B1L.F3 (forward primer)
- refseq_HSD11B1L.R3 (reverse primer)
- Single exon skipping
Frame 0
PAGRRRPGGHCCPATLRRWVPEARPLPGRRWASSHVTRALREGVRSGTRQEAAEKRGPWGRSRGSQP
GTPPPLDSGGN*GLSGPTQDHEGASPHRAGGPV
LRLLLG*QLRPSQPPGSASAADRGQRWCW*GAG
LSLRASGLPPGAHCPH*GSPAEGGLDYLVLNHIG
GAPAGTRARSPQATRWLMQVNFVSYVQLTSRAL
PSLTDSKGSLVVVSSLLGRVPTSFSTPYSAAKF
ALDGFFGSLRRELDVQDVNVAITMCVLGLRDRAS
AAEAVRGVTRVKAAPGPKAALAVIRGGATRAAG
VFYPWRFRLLCLLRRWLPRPRAWFIRQELNVTA
AAA*APGGAPPVPDGNVPPSNCPWSQNTHRDTPE
RVATAQDEVIKTEKQNREKRRAPGTSHGLGGAG
APC*APLSGTCKASPVWP*LMT*LLPFCR*GN*
GSERPRHP*ATHGPLSISCLRL*VPDLFFPFIPS
GRNPPNSCQLPLAGVSGTLHTCRGVYTVRLPGG
RV*AGGGGKECVLSWTQPLVRE*KLFFSC
Frame 1
LLGGEDQAGTVARRRSGAGSPRPGPSPGGGGLRVT*PVPYGRGCGRGPGRRRPRREDRGGVRVAPS
PGPHPRWTVGETEA*AGPHRTMKVLLLTGLGALF
FAYYWDDNFDPASLQGARVLLTGANAGVGEELA
YHYARLGSHLVLTAHTEALLQKAGWTTSC*TTS
AAPRPAREPAAPRQLAGSCR*TL*ATCN*RRGRC
PA*RTARAPWWWCPRCSAACPRRSPLPTRRPSL
RWTASSAPCGGSWTCRT*TWPSPCASWASEIAP
PPPRQSGESRGSRRPRGPRQPWP*SAAAPRARPA
SSTRGVSACCACSGAGYRARGPGLSARSSTSRP
RQPEHRGVPLQSQTAMFLPPTVPGARTLTETPL
RGWPQPKMKSSRQKSKTEKNDGHLEPVTAWEVQV
PRVRRLCRGLARPHLFGHD**RDCFHFADEETK
AQRGHATLEPPMDPSPSPACAFKSLIYSFHSFH
LGGTPPTPASFP*LGSLVLFTPAGASTLFVYLVA
GSEREEEGKSVF*AGPSLLFENKNSSSLA
Frame 2
CWEAKTRRALLPGDAPALGPRGPAPPREEVGFESRDPCPTGGGAVGDPAGGGREERTVGAFAWLPA
RDPTPAGQWGKLRPERAHTGP*RCFSSQGWGPC
SSPIIGMTTSTQPASRERECC*QGPTLVLVRSWP
ITTRVWAPTWCSLPTLRLSCRRRAGLPRAEPHR
RRPGRHASPQPPGNSLAHAGKLCELRATDVAGA
AQPDGQQGLPGGGVLAARPRAHVVLHSLLGGQVC
AGRLLRLPAAGAGRAGRERGHHHVRPGPPRSRL
RRRGSQGSHEGQGGPGAQGSPGRDPRRRHARGR
RLLPVAFPPAVLAPALATAPAGLVYPPGAQRHGR
GSLSTGGCPSSPRRQCSSLQLSLEPEHSQRHP*
EGGHSPR*SHQDRKAKPRKTTGTWNQSRLGRCR
CPVLGAFVGDLQGLTCLAMIDDVTASILQMRKLR
LREATPPLSHPWTPLHLLPAPLSP*FILSIHSI
WEEPPQLLPASPSWGLWYSSHLQGRLHCSSTWW
QGLSGRRRERVCSELDPASCSRIKTLLLL
Sequence NM_198704
CCTGCTGGGAGGCGAAGACCAGGCGGGCACTGTTGCCCGGCGACGCTCCGGCGCTGGGTCCCCGAGGCCCGGCCCCTCCCCGGGAGGAGGTGGGCTTCGAGTCACGTGACCCGTGCCCTACGGGAGGGGGTGCGGTCGGGGACCCGGCAGGAGGCGGCCGAGAAGAGAGGACCGTGGGGGCGTTCGCGTGGCTCCCAGCC
CGGGACCCCACCCCCGCTGGACAGTGGGGGAAACTGAGGCCTGAGCGGAGTGACCACCCCGGCTATGGCCAGCCAGCCTCCAGGGAGCGCGAGTGCTGCT
GACAGGGGCCAACGCTGGTGTTGGTGAGGAGCTGGCCTATCACTACGCGCGTCTGGGCTCCCACCTGGTGCTCACTGCCCACACTGAGGCTCTCCTGCAG
AAGGCGGGCTGGACTACCTCGTGCTGAACCACATCGGCGGCGCCCCGGCCGGCACGCGAGCCCGCAGCCCCCAGGCAACTCGCTGGCTCATGCAGGTAAA
CTTTGTGAGCTACGTGCAACTGACGTCGCGGGCGCTGCCCAGCCTGACGGACAGCAAGGGCTCCCTGGTGGTGGTGTCCTCGCTGCTCGGCCGCGTGCCC
ACGTCGTTCTCCACTCCCTACTCGGCGGCCAAGTTTGCGCTGGACGGCTTCTTCGGCTCCCTGCGGCGGGAGCTGGACGTGCAGGACGTGAACGTGGCCA
TCACCATGTGCGTCCTGGGCCTCCGAGATCGCGCCTCCGCCGCCGAGGCAGTCAGGAGCTCAACGTCACGGCCGCGGCAGCCTGAGCACCGGGGGGTGCC
CCTCCAGTCCCAGACGGCAATGTTCCTCCCTCCAACTGTCCCTGGAGCCAGAACACTCACAGAGACACCCCTGAGAGGGTGGCCACAGCCCAAGATGAAG
TCATCAAGACAGAAAAGCAAAACCGAGAAAAACGACGGGCACCTGGAACCAGTCACGGCTTGGGAGGTGCAGGTGCCCCGTGTTAGGCGCCTTTGTCGGG
GACTTGCAAGGCCTCACCTGTTTGGCCATGATTGATGACGTGACTGCTTCCATTTTGCAGATGAGGAAACTAAGGCTCAGAGAGGCCACGCCACCCTTGA
GCCACCCATGGACCCCTCTCCATCTCCTGCCTGCGCCTTTAAGTCCCTGATTTATTCTTTCCATTCATTCCATCTGGGAGGAACCCCCCCAACTCCTGCC
AGCTTCCCCTAGCTGGGGTCTCTGGTACTCTTCACACCTGCAGGGGCGTCTACACTGTTCGTCTACCTGGTGGCAGGGTCTGAGCGGGAGGAGGAGGGAA
AGAGTGTGTTCTGAGCTGGACCCAGCCTCTTGTTCGAGAATAAAAACTCTTCTTCTCTTGCA
- refseq_HSD11B1L.F3 (forward primer)
- refseq_HSD11B1L.R3 (reverse primer)
- Single exon skipping
Frame 0
PAGRRRPGGHCCPATLRRWVPEARPLPGRRWASSHVTRALREGVRSGTRQEAAEKRGPWGRSRGSQP
GTPPPLDSGGN*GLSGVTTPAMASQPPGSASAA
DRGQRWCW*GAGLSLRASGLPPGAHCPH*GSPA
EGGLDYLVLNHIGGAPAGTRARSPQATRWLMQVN
FVSYVQLTSRALPSLTDSKGSLVVVSSLLGRVP
TSFSTPYSAAKFALDGFFGSLRRELDVQDVNVA
ITMCVLGLRDRASAAEAVRSSTSRPRQPEHRGVP
LQSQTAMFLPPTVPGARTLTETPLRGWPQPKMK
SSRQKSKTEKNDGHLEPVTAWEVQVPRVRRLCR
GLARPHLFGHD**RDCFHFADEETKAQRGHATLE
PPMDPSPSPACAFKSLIYSFHSFHLGGTPPTPA
SFP*LGSLVLFTPAGASTLFVYLVAGSEREEEG
KSVF*AGPSLLFENKNSSSLA
Frame 1
LLGGEDQAGTVARRRSGAGSPRPGPSPGGGGLRVT*PVPYGRGCGRGPGRRRPRREDRGGVRVAPS
PGPHPRWTVGETEA*AE*PPRLWPASLQGARVLL
TGANAGVGEELAYHYARLGSHLVLTAHTEALLQ
KAGWTTSC*TTSAAPRPAREPAAPRQLAGSCR*
TL*ATCN*RRGRCPA*RTARAPWWWCPRCSAACP
RRSPLPTRRPSLRWTASSAPCGGSWTCRT*TWP
SPCASWASEIAPPPPRQSGAQRHGRGSLSTGGC
PSSPRRQCSSLQLSLEPEHSQRHP*EGGHSPR*S
HQDRKAKPRKTTGTWNQSRLGRCRCPVLGAFVG
DLQGLTCLAMIDDVTASILQMRKLRLREATPPL
SHPWTPLHLLPAPLSP*FILSIHSIWEEPPQLLP
ASPSWGLWYSSHLQGRLHCSSTWWQGLSGRRRE
RVCSELDPASCSRIKTLLLL
Frame 2
CWEAKTRRALLPGDAPALGPRGPAPPREEVGFESRDPCPTGGGAVGDPAGGGREERTVGAFAWLPA
RDPTPAGQWGKLRPERSDHPGYGQPASRERECC
*QGPTLVLVRSWPITTRVWAPTWCSLPTLRLSCR
RRAGLPRAEPHRRRPGRHASPQPPGNSLAHAGK
LCELRATDVAGAAQPDGQQGLPGGGVLAARPRA
HVVLHSLLGGQVCAGRLLRLPAAGAGRAGRERGH
HHVRPGPPRSRLRRRGSQELNVTAAAA*APGGA
PPVPDGNVPPSNCPWSQNTHRDTPERVATAQDE
VIKTEKQNREKRRAPGTSHGLGGAGAPC*APLSG
TCKASPVWP*LMT*LLPFCR*GN*GSERPRHP*
ATHGPLSISCLRL*VPDLFFPFIPSGRNPPNSC
QLPLAGVSGTLHTCRGVYTVRLPGGRV*AGGGGK
ECVLSWTQPLVRE*KLFFSC
Sequence NM_198707
CCTGCTGGGAGGCGAAGACCAGGCGGGCACTGTTGCCCGGCGACGCTCCGGCGCTGGGTCCCCGAGGCCCGGCCCCTCCCCGGGAGGAGGTGGGCTTCGAGTCACGTGACCCGTGCCCTACGGGAGGGGGTGCGGTCGGGGACCCGGCAGGAGGCGGCCGAGAAGAGAGGACCGTGGGGGCGTTCGCGTGGCTCCCAGCC
CGGGACCCCACCCCCGCTGGACAGTGGGGGAAACTGAGGCCTGAGCGGAGTGACCACCCCGGCTATGGCCAGCCAGCCTCCAGGGAGCGCGAGTGCTGCT
GACAGGGGCCAACGCTGGTGTTGGTGAGGAGCTGGCCTATCACTACGCGCGTCTGGGCTCCCACCTGGTGCTCACTGCCCACACTGAGGCTCTCCTGCAG
AAGGTGGTAGGGAACTGCCGGAAGCTGGGCGCCCCCAAGGTCTTCTACATCGCGGCGGACATGGCCTCCCCTGAGGCGCCCGAGAGCGTGGTGCAGTTTG
CGCTGGACAAGCTGGGCGGGCTGGACTACCTCGTGCTGAACCACATCGGCGGCGCCCCGGCCGGCACGCGAGCCCGCAGCCCCCAGGCAACTCGCTGGCT
CATGCAGGTAAACTTTGTGAGCTACGTGCAACTGACGTCGCGGGCGCTGCCCAGCCTGACGGACAGCAAGGGCTCCCTGGTGGTGGTGTCCTCGCTGCTC
GGCCGCGTGCCCACGTCGTTCTCCACTCCCTACTCGGCGGCCAAGTTTGCGCTGGACGGCTTCTTCGGCTCCCTGCGGCGGGAGCTGGACGTGCAGGACG
TGAACGTGGCCATCACCATGTGCGTCCTGGGCCTCCGAGATCGCGCCTCCGCCGCCGAGGCAGTCAGGGGAGTCACGAGGGTCAAGGCGGCCCCGGGGCC
CAAGGCAGCCCTGGCCGTGATCCGCGGCGGCGCCACGCGCGCGGCCGGCGTCTTCTACCCGTGGCGTTTCCGCCTGCTGTGCTTGCTCCGGCGCTGGCTA
CCGCGCCCGCGGGCCTGGTTTATCCGCCAGGAGCTCAACGTCACGGCCGCGGCAGCCTGAGCACCGGGGGGTGCCCCTCCAGTCCCAGACGGCAATGTTC
CTCCCTCCAACTGTCCCTGGAGCCAGAACACTCACAGAGACACCCCTGAGAGGGTGGCCACAGCCCAAGATGAAGTCATCAAGACAGAAAAGCAAAACCG
AGAAAAACGACGGGCACCTGGAACCAGTCACGGCTTGGGAGGTGCAGGTGCCCCGTGTTAGGCGCCTTTGTCGGGGACTTGCAAGGCCTCACCTGTTTGG
CCATGATTGATGACGTGACTGCTTCCATTTTGCAGATGAGGAAACTAAGGCTCAGAGAGGCCACGCCACCCTTGAGCCACCCATGGACCCCTCTCCATCT
CCTGCCTGCGCCTTTAAGTCCCTGATTTATTCTTTCCATTCATTCCATCTGGGAGGAACCCCCCCAACTCCTGCCAGCTTCCCCTAGCTGGGGTCTCTGG
TACTCTTCACACCTGCAGGGGCGTCTACACTGTTCGTCTACCTGGTGGCAGGGTCTGAGCGGGAGGAGGAGGGAAAGAGTGTGTTCTGAGCTGGACCCAG
CCTCTTGTTCGAGAATAAAAACTCTTCTTCTCTTGCA
- refseq_HSD11B1L.F3 (forward primer)
- refseq_HSD11B1L.R3 (reverse primer)
- Single exon skipping
Frame 0
PAGRRRPGGHCCPATLRRWVPEARPLPGRRWASSHVTRALREGVRSGTRQEAAEKRGPWGRSRGSQP
GTPPPLDSGGN*GLSGVTTPAMASQPPGSASAA
DRGQRWCW*GAGLSLRASGLPPGAHCPH*GSPA
EGGRELPEAGRPQGLLHRGGHGLP*GARERGAVC
AGQAGRAGLPRAEPHRRRPGRHASPQPPGNSLA
HAGKLCELRATDVAGAAQPDGQQGLPGGGVLAA
RPRAHVVLHSLLGGQVCAGRLLRLPAAGAGRAGR
ERGHHHVRPGPPRSRLRRRGSQGSHEGQGGPGA
QGSPGRDPRRRHARGRRLLPVAFPPAVLAPALA
TAPAGLVYPPGAQRHGRGSLSTGGCPSSPRRQCS
SLQLSLEPEHSQRHP*EGGHSPR*SHQDRKAKP
RKTTGTWNQSRLGRCRCPVLGAFVGDLQGLTCL
AMIDDVTASILQMRKLRLREATPPLSHPWTPLHL
LPAPLSP*FILSIHSIWEEPPQLLPASPSWGLW
YSSHLQGRLHCSSTWWQGLSGRRRERVCSELDP
ASCSRIKTLLLL
Frame 1
LLGGEDQAGTVARRRSGAGSPRPGPSPGGGGLRVT*PVPYGRGCGRGPGRRRPRREDRGGVRVAPS
PGPHPRWTVGETEA*AE*PPRLWPASLQGARVLL
TGANAGVGEELAYHYARLGSHLVLTAHTEALLQ
KVVGNCRKLGAPKVFYIAADMASPEAPESVVQF
ALDKLGGLDYLVLNHIGGAPAGTRARSPQATRWL
MQVNFVSYVQLTSRALPSLTDSKGSLVVVSSLL
GRVPTSFSTPYSAAKFALDGFFGSLRRELDVQD
VNVAITMCVLGLRDRASAAEAVRGVTRVKAAPGP
KAALAVIRGGATRAAGVFYPWRFRLLCLLRRWL
PRPRAWFIRQELNVTAAAA*APGGAPPVPDGNV
PPSNCPWSQNTHRDTPERVATAQDEVIKTEKQNR
EKRRAPGTSHGLGGAGAPC*APLSGTCKASPVW
P*LMT*LLPFCR*GN*GSERPRHP*ATHGPLSI
SCLRL*VPDLFFPFIPSGRNPPNSCQLPLAGVSG
TLHTCRGVYTVRLPGGRV*AGGGGKECVLSWTQ
PLVRE*KLFFSC
Frame 2
CWEAKTRRALLPGDAPALGPRGPAPPREEVGFESRDPCPTGGGAVGDPAGGGREERTVGAFAWLPA
RDPTPAGQWGKLRPERSDHPGYGQPASRERECC
*QGPTLVLVRSWPITTRVWAPTWCSLPTLRLSCR
RW*GTAGSWAPPRSSTSRRTWPPLRRPRAWCSL
RWTSWAGWTTSC*TTSAAPRPAREPAAPRQLAG
SCR*TL*ATCN*RRGRCPA*RTARAPWWWCPRCS
AACPRRSPLPTRRPSLRWTASSAPCGGSWTCRT
*TWPSPCASWASEIAPPPPRQSGESRGSRRPRG
PRQPWP*SAAAPRARPASSTRGVSACCACSGAGY
RARGPGLSARSSTSRPRQPEHRGVPLQSQTAMF
LPPTVPGARTLTETPLRGWPQPKMKSSRQKSKT
EKNDGHLEPVTAWEVQVPRVRRLCRGLARPHLFG
HD**RDCFHFADEETKAQRGHATLEPPMDPSPS
PACAFKSLIYSFHSFHLGGTPPTPASFP*LGSL
VLFTPAGASTLFVYLVAGSEREEEGKSVF*AGPS
LLFENKNSSSLA
Sequence NM_198533
CCTGCTGGGAGGCGAAGACCAGGCGGGCACTGTTGCCCGGCGACGCTCCGGCGCTGGGTCCCCGAGGCCCGGCCCCTCCCCGGGAGGAGGTGGGCTTCGAGTCACGTGACCCGTGCCCTACGGGAGGGGGTGCGGTCGGGGACCCGGCAGGAGGCGGCCGAGAAGAGAGGACCGTGGGGGCGTTCGCGTGGCTCCCAGCC
CGGGACCCCACCCCCGCTGGACAGTGGGGGAAACTGAGGCCTGAGCGGGCCCACACAGGACCATGAAGGTGCTTCTCCTCACAGGGCTGGGGGCCCTGTT
CTTCGCCTATTATTGGGATGACAACTTCGACCCAGCCAGCCTCCAGGGAGCGCGAGTGCTGCTGACAGGGGCCAACGCTGGTGTTGGTGAGGAGCTGGCC
TATCACTACGCGCGTCTGGGCTCCCACCTGGTGCTCACTGCCCACACTGAGGCTCTCCTGCAGAAGGTGGTAGGGAACTGCCGGAAGCTGGGCGCCCCCA
AGGTCTTCTACATCGCGGCGGACATGGCCTCCCCTGAGGCGCCCGAGAGCGTGGTGCAGTTTGCGCTGGACAAGCTGGGCGGGCTGGACTACCTCGTGCT
GAACCACATCGGCGGCGCCCCGGCCGGCACGCGAGCCCGCAGCCCCCAGGCAACTCGCTGGCTCATGCAGGTAAACTTTGTGAGCTACGTGCAACTGACG
TCGCGGGCGCTGCCCAGCCTGACGGACAGCAAGGGCTCCCTGGTGGTGGTGTCCTCGCTGCTCGGCCGCGTGCCCACGTCGTTCTCCACTCCCTACTCGG
CGGCCAAGTTTGCGCTGGACGGCTTCTTCGGCTCCCTGCGGCGGGAGCTGGACGTGCAGGACGTGAACGTGGCCATCACCATGTGCGTCCTGGGCCTCCG
AGATCGCGCCTCCGCCGCCGAGGCAGTCAGGAGCTCAACGTCACGGCCGCGGCAGCCTGAGCACCGGGGGGTGCCCCTCCAGTCCCAGACGGCAATGTTC
CTCCCTCCAACTGTCCCTGGAGCCAGAACACTCACAGAGACACCCCTGAGAGGGTGGCCACAGCCCAAGATGAAGTCATCAAGACAGAAAAGCAAAACCG
AGAAAAACGACGGGCACCTGGAACCAGTCACGGCTTGGGAGGTGCAGGTGCCCCGTGTTAGGCGCCTTTGTCGGGGACTTGCAAGGCCTCACCTGTTTGG
CCATGATTGATGACGTGACTGCTTCCATTTTGCAGATGAGGAAACTAAGGCTCAGAGAGGCCACGCCACCCTTGAGCCACCCATGGACCCCTCTCCATCT
CCTGCCTGCGCCTTTAAGTCCCTGATTTATTCTTTCCATTCATTCCATCTGGGAGGAACCCCCCCAACTCCTGCCAGCTTCCCCTAGCTGGGGTCTCTGG
TACTCTTCACACCTGCAGGGGCGTCTACACTGTTCGTCTACCTGGTGGCAGGGTCTGAGCGGGAGGAGGAGGGAAAGAGTGTGTTCTGAGCTGGACCCAG
CCTCTTGTTCGAGAATAAAAACTCTTCTTCTCTTGCA
- refseq_HSD11B1L.F3 (forward primer)
- refseq_HSD11B1L.R3 (reverse primer)
- Single exon skipping
Frame 0
PAGRRRPGGHCCPATLRRWVPEARPLPGRRWASSHVTRALREGVRSGTRQEAAEKRGPWGRSRGSQP
GTPPPLDSGGN*GLSGPTQDHEGASPHRAGGPV
LRLLLG*QLRPSQPPGSASAADRGQRWCW*GAG
LSLRASGLPPGAHCPH*GSPAEGGRELPEAGRPQ
GLLHRGGHGLP*GARERGAVCAGQAGRAGLPRA
EPHRRRPGRHASPQPPGNSLAHAGKLCELRATD
VAGAAQPDGQQGLPGGGVLAARPRAHVVLHSLLG
GQVCAGRLLRLPAAGAGRAGRERGHHHVRPGPP
RSRLRRRGSQELNVTAAAA*APGGAPPVPDGNV
PPSNCPWSQNTHRDTPERVATAQDEVIKTEKQNR
EKRRAPGTSHGLGGAGAPC*APLSGTCKASPVW
P*LMT*LLPFCR*GN*GSERPRHP*ATHGPLSI
SCLRL*VPDLFFPFIPSGRNPPNSCQLPLAGVSG
TLHTCRGVYTVRLPGGRV*AGGGGKECVLSWTQ
PLVRE*KLFFSC
Frame 1
LLGGEDQAGTVARRRSGAGSPRPGPSPGGGGLRVT*PVPYGRGCGRGPGRRRPRREDRGGVRVAPS
PGPHPRWTVGETEA*AGPHRTMKVLLLTGLGALF
FAYYWDDNFDPASLQGARVLLTGANAGVGEELA
YHYARLGSHLVLTAHTEALLQKVVGNCRKLGAP
KVFYIAADMASPEAPESVVQFALDKLGGLDYLVL
NHIGGAPAGTRARSPQATRWLMQVNFVSYVQLT
SRALPSLTDSKGSLVVVSSLLGRVPTSFSTPYS
AAKFALDGFFGSLRRELDVQDVNVAITMCVLGLR
DRASAAEAVRSSTSRPRQPEHRGVPLQSQTAMF
LPPTVPGARTLTETPLRGWPQPKMKSSRQKSKT
EKNDGHLEPVTAWEVQVPRVRRLCRGLARPHLFG
HD**RDCFHFADEETKAQRGHATLEPPMDPSPS
PACAFKSLIYSFHSFHLGGTPPTPASFP*LGSL
VLFTPAGASTLFVYLVAGSEREEEGKSVF*AGPS
LLFENKNSSSLA
Frame 2
CWEAKTRRALLPGDAPALGPRGPAPPREEVGFESRDPCPTGGGAVGDPAGGGREERTVGAFAWLPA
RDPTPAGQWGKLRPERAHTGP*RCFSSQGWGPC
SSPIIGMTTSTQPASRERECC*QGPTLVLVRSWP
ITTRVWAPTWCSLPTLRLSCRRW*GTAGSWAPP
RSSTSRRTWPPLRRPRAWCSLRWTSWAGWTTSC
*TTSAAPRPAREPAAPRQLAGSCR*TL*ATCN*R
RGRCPA*RTARAPWWWCPRCSAACPRRSPLPTR
RPSLRWTASSAPCGGSWTCRT*TWPSPCASWAS
EIAPPPPRQSGAQRHGRGSLSTGGCPSSPRRQCS
SLQLSLEPEHSQRHP*EGGHSPR*SHQDRKAKP
RKTTGTWNQSRLGRCRCPVLGAFVGDLQGLTCL
AMIDDVTASILQMRKLRLREATPPLSHPWTPLHL
LPAPLSP*FILSIHSIWEEPPQLLPASPSWGLW
YSSHLQGRLHCSSTWWQGLSGRRRERVCSELDP
ASCSRIKTLLLL
Sequence NM_198706
CCTGCTGGGAGGCGAAGACCAGGCGGGCACTGTTGCCCGGCGACGCTCCGGCGCTGGGTCCCCGAGGCCCGGCCCCTCCCCGGGAGGAGGTGGGCTTCGAGTCACGTGACCCGTGCCCTACGGGAGGGGGTGCGGTCGGGGACCCGGCAGGAGGCGGCCGAGAAGAGAGGACCGTGGGGGCGTTCGCGTGGCTCCCAGCC
CGGGACCCCACCCCCGCTGGACAGTGGGGGAAACTGAGGCCTGAGCGGGCCCACACAGGACCATGAAGGTGCTTCTCCTCACAGGGCTGGGGGCCCTGTT
CTTCGCCTATTATTGGGATGACAACTTCGACCCAGCCAGCCTCCAGGGAGCGCGAGTGCTGCTGACAGGGGCCAACGCTGGTGTTGGTGAGGAGCTGGCC
TATCACTACGCGCGTCTGGGCTCCCACCTGGTGCTCACTGCCCACACTGAGGCTCTCCTGCAGAAGGTGGTAGGGAACTGCCGGAAGCTGGGCGCCCCCA
AGGTCTTCTACATCGCGGCGGACATGGCCTCCCCTGAGGCGCCCGAGAGCGTGGTGCAGTTTGCGCTGGACAAGCTGGGCGGGCTGGACTACCTCGTGCT
GAACCACATCGGCGGCGCCCCGGCCGGCACGCGAGCCCGCAGCCCCCAGGCAACTCGCTGGCTCATGCAGGTAAACTTTGTGAGCTACGTGCAACTGACG
TCGCGGGCGCTGCCCAGCCTGACGGACAGCAAGGGCTCCCTGGTGGTGGTGTCCTCGCTGCTCGGCCGCGTGCCCACGTCGTTCTCCACTCCCTACTCGG
CGGCCAAGTTTGCGCTGGACGGCTTCTTCGGCTCCCTGCGGCGGGAGCTGGACGTGCAGGACGTGAACGTGGCCATCACCATGTGCGTCCTGGGCCTCCG
AGATCGCGCCTCCGCCGCCGAGGCAGTCAGGGGAGTCACGAGGGTCAAGGCGGCCCCGGGGCCCAAGGCAGCCCTGGCCGTGATCCGCGGCGGCGCCACG
CGCGCGGCCGGCGTCTTCTACCCGTGGCGTTTCCGCCTGCTGTGCTTGCTCCGGCGCTGGCTACCGCGCCCGCGGGCCTGGTTTATCCGCCAGGAGCTCA
ACGTCACGGCCGCGGCAGCCTGAGCACCGGGGGGTGCCCCTCCAGTCCCAGACGGCAATGTTCCTCCCTCCAACTGTCCCTGGAGCCAGAACACTCACAG
AGACACCCCTGAGAGGGTGGCCACAGCCCAAGATGAAGTCATCAAGACAGAAAAGCAAAACCGAGAAAAACGACGGGCACCTGGAACCAGTCACGGCTTG
GGAGGTGCAGGTGCCCCGTGTTAGGCGCCTTTGTCGGGGACTTGCAAGGCCTCACCTGTTTGGCCATGATTGATGACGTGACTGCTTCCATTTTGCAGAT
GAGGAAACTAAGGCTCAGAGAGGCCACGCCACCCTTGAGCCACCCATGGACCCCTCTCCATCTCCTGCCTGCGCCTTTAAGTCCCTGATTTATTCTTTCC
ATTCATTCCATCTGGGAGGAACCCCCCCAACTCCTGCCAGCTTCCCCTAGCTGGGGTCTCTGGTACTCTTCACACCTGCAGGGGCGTCTACACTGTTCGT
CTACCTGGTGGCAGGGTCTGAGCGGGAGGAGGAGGGAAAGAGTGTGTTCTGAGCTGGACCCAGCCTCTTGTTCGAGAATAAAAACTCTTCTTCTCTTGCA
- refseq_HSD11B1L.F3 (forward primer)
- refseq_HSD11B1L.R3 (reverse primer)
- Single exon skipping
Frame 0
PAGRRRPGGHCCPATLRRWVPEARPLPGRRWASSHVTRALREGVRSGTRQEAAEKRGPWGRSRGSQP
GTPPPLDSGGN*GLSGPTQDHEGASPHRAGGPV
LRLLLG*QLRPSQPPGSASAADRGQRWCW*GAG
LSLRASGLPPGAHCPH*GSPAEGGRELPEAGRPQ
GLLHRGGHGLP*GARERGAVCAGQAGRAGLPRA
EPHRRRPGRHASPQPPGNSLAHAGKLCELRATD
VAGAAQPDGQQGLPGGGVLAARPRAHVVLHSLLG
GQVCAGRLLRLPAAGAGRAGRERGHHHVRPGPP
RSRLRRRGSQGSHEGQGGPGAQGSPGRDPRRRH
ARGRRLLPVAFPPAVLAPALATAPAGLVYPPGAQ
RHGRGSLSTGGCPSSPRRQCSSLQLSLEPEHSQ
RHP*EGGHSPR*SHQDRKAKPRKTTGTWNQSRL
GRCRCPVLGAFVGDLQGLTCLAMIDDVTASILQM
RKLRLREATPPLSHPWTPLHLLPAPLSP*FILS
IHSIWEEPPQLLPASPSWGLWYSSHLQGRLHCS
STWWQGLSGRRRERVCSELDPASCSRIKTLLLL
Frame 1
LLGGEDQAGTVARRRSGAGSPRPGPSPGGGGLRVT*PVPYGRGCGRGPGRRRPRREDRGGVRVAPS
PGPHPRWTVGETEA*AGPHRTMKVLLLTGLGALF
FAYYWDDNFDPASLQGARVLLTGANAGVGEELA
YHYARLGSHLVLTAHTEALLQKVVGNCRKLGAP
KVFYIAADMASPEAPESVVQFALDKLGGLDYLVL
NHIGGAPAGTRARSPQATRWLMQVNFVSYVQLT
SRALPSLTDSKGSLVVVSSLLGRVPTSFSTPYS
AAKFALDGFFGSLRRELDVQDVNVAITMCVLGLR
DRASAAEAVRGVTRVKAAPGPKAALAVIRGGAT
RAAGVFYPWRFRLLCLLRRWLPRPRAWFIRQEL
NVTAAAA*APGGAPPVPDGNVPPSNCPWSQNTHR
DTPERVATAQDEVIKTEKQNREKRRAPGTSHGL
GGAGAPC*APLSGTCKASPVWP*LMT*LLPFCR
*GN*GSERPRHP*ATHGPLSISCLRL*VPDLFFP
FIPSGRNPPNSCQLPLAGVSGTLHTCRGVYTVR
LPGGRV*AGGGGKECVLSWTQPLVRE*KLFFSC
Frame 2
CWEAKTRRALLPGDAPALGPRGPAPPREEVGFESRDPCPTGGGAVGDPAGGGREERTVGAFAWLPA
RDPTPAGQWGKLRPERAHTGP*RCFSSQGWGPC
SSPIIGMTTSTQPASRERECC*QGPTLVLVRSWP
ITTRVWAPTWCSLPTLRLSCRRW*GTAGSWAPP
RSSTSRRTWPPLRRPRAWCSLRWTSWAGWTTSC
*TTSAAPRPAREPAAPRQLAGSCR*TL*ATCN*R
RGRCPA*RTARAPWWWCPRCSAACPRRSPLPTR
RPSLRWTASSAPCGGSWTCRT*TWPSPCASWAS
EIAPPPPRQSGESRGSRRPRGPRQPWP*SAAAPR
ARPASSTRGVSACCACSGAGYRARGPGLSARSS
TSRPRQPEHRGVPLQSQTAMFLPPTVPGARTLT
ETPLRGWPQPKMKSSRQKSKTEKNDGHLEPVTAW
EVQVPRVRRLCRGLARPHLFGHD**RDCFHFAD
EETKAQRGHATLEPPMDPSPSPACAFKSLIYSF
HSFHLGGTPPTPASFP*LGSLVLFTPAGASTLFV
YLVAGSEREEEGKSVF*AGPSLLFENKNSSSLA
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