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Sequences

Sequence NM_006636

GGGGCCTGCCACGAGGCCGCAGTATAACCGCGTGGCCCGCGCGCGCGCTTCCCTCCCGGCGCAGTCACCGGCGCGGTCTATGGCTGCGACTTCTCTAATG TCTGCTTTGGCTGCCCGGCTGCTGCAGCCCGCGCACAGCTGCTCCCTTCGCCTTCGCCCTTTCCACCTCGCGGCAGTTCGAAATGAAGCTGTTGTCATTT CTGGAAGGAAACTGGCCCAGCAGATCAAGCAGGAAGTGCGGCAGGAGGTAGAAGAGTGGGTGGCCTCAGGCAACAAACGGCCACACCTGAGTGTGATCCT GGTTGGCGAGAATCCTGCAAGTCACTCCTATGTCCTCAACAAAACCAGGGCAGCTGCAGTTGTGGGAATCAACAGTGAGACAATTATGAAACCAGCTTCA ATTTCAGAGGAAGAATTGTTGAATTTAATCAATAAACTGAATAATGATGATAATGTAGATGGCCTCCTTGTTCAGTTGCCTCTTCCAGAGCATATTGATG AGAGAAGGATCTGCAATGCTGTTTCTCCAGACAAGGATGTTGATGGCTTTCATGTAATTAATGTAGGACGAATGTGTTTGGATCAGTATTCCATGTTACC GGCTACTCCATGGGGTGTGTGGGAAATAATCAAGCGAACTGGCATTCCAACCCTAGGGAAGAATGTGGTTGTGGCTGGAAGGTCAAAAAACGTTGGAATG CCCATTGCAATGTTACTGCACACAGATGGGGCGCATGAACGTCCCGGAGGTGATGCCACTGTTACAATATCTCATCGATATACTCCCAAAGAGCAGTTGA AGAAACATACAATTCTTGCAGATATTGTAATATCTGCTGCAGGTATTCCAAATCTGATCACAGCAGATATGATCAAGGAAGGAGCAGCAGTCATTGATGT GGGAATAAATAGAGTTCACGATCCTGTAACTGCCAAACCCAAGTTGGTTGGAGATGTGGATTTTGAAGGAGTCAGACAAAAAGCTGGGTATATCACTCCA GTTCCTGGAGGTGTTGGCCCCATGACAGTGGCAATGCTAATGAAGAATACCATTATTGCTGCAAAAAAGGTGCTGAGGCTTGAAGAGCGAGAAGTGCTGA AGTCTAAAGAGCTTGGGGTAGCCACTAATTAACTACTGTGTCTTCTGTGTCACAAACAGCACTCCAGGCCAGCTCAAGAAGCAAAGCAGGCCAATAGAAA TGCAATATTTTTAATTTATTCTACTGAAATGGTTTAAAATGATGCCTTGTATTTATTGAAAGCTTAAATGGGTGGGTGTTTCTGCACATACCTCTGCAGT ACCTCACCAGGGAGCATTCCAGTATCATGCAGGGTCCTGTGATCTAGCCAGGAGCAGCCATTAACCTAGTGATTAATATGGGAGACATTACCATATGGAG GATGGATGCTTCACTTTGTCAAGCACCTCAGTTACACATTCGCCTTTTCTAGGATTGCATTTCCCAAGTGCTATTGCAATAACAGTTGATACTCATTTTA GGTACCAAACCTTTTGAGTTCAACTGATCAAACCAAAGGAAAAGTGTTGCTAGAGAAAATTAGGGAAAAGGTGAAAAAGAAAAAATGGTAGTAATTGAGC AGAAAAAAATTAATTTATATATGTATTGATTGGCAACCAGATTTATCTAAGTAGAACTGAATTGGCTAGGAAAAAAGAAAAACTGCATGTTAATCATTTT CCTAAGCTGTCCTTTTGAGGCTTAGTCAGTTTATTGGGAAAATGTTTAGGATTATTCCTTGCTATTAGTACTCATTTTATGTATGTTACCCTTCAGTAAG TTCTCCCCATTTTAGTTTTCTAGGACTGAAAGGATTCTTTTCTACATTATACATGTGTGTTGTCATATTTGGCTTTTGCTATATACTTTAACTTCATTGT TAAATTTTTGTATTGTATAGTTTCTTTGGTGTATCTTAAAACCTATTTTTGAAAAACAAACTTGGCTTGATAATCATTTGGGCAGCTTGGGTAAGTACGC AACTTACTTTTCCACCAAAGAACTGTCAGCAGCTGCCTGCTTTTCTGTGATGTATGTATCCTGTTGACTTTTCCAGAAATTTTTTAAGAGTTTGAGTTAC TATTGAATTTAATCAGACTTTCTGATTAAAGGGTTTTCTTTCTTTTTTAATAAAACACATCTGTCTGGTATGGTATGAATTTCTGAAA

refseq_MTHFD2.F1 (forward primer)
refseq_MTHFD2.R1 (reverse primer)
Single exon skipping

Frame 0

GACHEAAV*PRGPRARFPPGAVTGAVYGCDFSN VCFGCPAAAARAQLLPSPSPFPPRGSSK*SCCHF WKETGPADQAGSAAGGRRVGGLRQQTATPECDP GWRESCKSLLCPQQNQGSCSCGNQQ*DNYETSF NFRGRIVEFNQ*TE****CRWPPCSVASSRAY** EKDLQCCFSRQGC*WLSCN*CRTNVFGSVFHVT GYSMGCVGNNQANWHSNPREECGCGWKVKKRWN AHCNVTAHRWGA*TSRR*CHCYNISSIYSQRAVE ETYNSCRYCNICCRYSKSDHSRYDQGRSSSH*C GNK*SSRSCNCQTQVGWRCGF*RSQTKSWVYHS SSWRCWPHDSGNANEEYHYCCKKGAEA*RARSAE V*RAWGSH*LTTVSSVSQTALQASSRSKAGQ*K CNIFNLFY*NGLK*CLVFIESLNGWVFLHIPLQ YLTREHSSIMQGPVI*PGAAINLVINMGDITIWR MDASLCQAPQLHIRLF*DCISQVLLQ*QLILIL GTKPFEFN*SNQRKSVAREN*GKGEKEKMVVIE QKKINLYMY*LATRFI*VELNWLGKKKNCMLIIF LSCPFEA*SVYWENV*DYSLLLVLILCMLPFSK FSPF*FSRTERILFYIIHVCCHIWLLLYTLTSL LNFCIV*FLWCILKPIFEKQTWLDNHLGSLGKYA TYFSTKELSAAACFSVMYVSC*LFQKFFKSLSY Y*I*SDFLIKGFSFFFNKTHLSGMV*ISE

Frame 1

GPATRPQYNRVARARASLPAQSPARSMAATSLM SALAARLLQPAHSCSLRLRPFHLAAVRNEAVVI SGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVIL VGENPASHSYVLNKTRAAAVVGINSETIMKPAS ISEEELLNLINKLNNDDNVDGLLVQLPLPEHID ERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLP ATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGM PIAMLLHTDGAHERPGGDATVTISHRYTPKEQL KKHTILADIVISAAGIPNLITADMIKEGAAVIDV GINRVHDPVTAKPKLVGDVDFEGVRQKAGYITP VPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVL KSKELGVATN*LLCLLCHKQHSRPAQEAKQANRN AIFLIYSTEMV*NDALYLLKA*MGGCFCTYLCS TSPGSIPVSCRVL*SSQEQPLT**LIWETLPYG GWMLHFVKHLSYTFAFSRIAFPKCYCNNS*YSF* VPNLLSSTDQTKGKVLLEKIREKVKKKKW**LS RKKLIYICIDWQPDLSK*N*IG*EKRKTAC*SF S*AVLLRLSQFIGKMFRIIPCY*YSFYVCYPSVS SPHFSFLGLKGFFSTLYMCVVIFGFCYIL*LHC *IFVLYSFFGVS*NLFLKNKLGLIIIWAAWVST QLTFPPKNCQQLPAFL*CMYPVDFSRNFLRV*VT IEFNQTF*LKGFLSFLIKHICLVWYEFLK

Frame 2

GLPRGRSITAWPARALPSRRSHRRGLWLRLL*C LLWLPGCCSPRTAAPFAFALSTSRQFEMKLLSF LEGNWPSRSSRKCGRR*KSGWPQATNGHT*V*S WLARILQVTPMSSTKPGQLQLWESTVRQL*NQLQ FQRKNC*I*SIN*IMMIM*MASLFSCLFQSILM REGSAMLFLQTRMLMAFM*LM*DECVWISIPCY RLLHGVCGK*SSELAFQP*GRMWLWLEGQKTLEC PLQCYCTQMGRMNVPEVMPLLQYLIDILPKSS* RNIQFLQIL*YLLQVFQI*SQQI*SRKEQQSLM WE*IEFTIL*LPNPSWLEMWILKESDKKLGISLQ FLEVLAP*QWQC**RIPLLLQKRC*GLKSEKC* SLKSLG*PLINYCVFCVTNSTPGQLKKQSRPIE MQYF*FILLKWFKMMPCIY*KLKWVGVSAHTSAV PHQGAFQYHAGSCDLARSSH*PSD*YGRHYHME DGCFTLSSTSVTHSPFLGLHFPSAIAITVDTHF RYQTF*VQLIKPKEKCC*RKLGKR*KRKNGSN*A EKN*FIYVLIGNQIYLSRTELARKKEKLHVNHF PKLSF*GLVSLLGKCLGLFLAISTHFMYVTLQ* VLPILVF*D*KDSFLHYTCVLSYLAFAIYFNFIV KFLYCIVSLVYLKTYF*KTNLA**SFGQLG*VR NLLFHQRTVSSCLLFCDVCILLTFPEIF*EFEL LLNLIRLSD*RVFFLF**NTSVWYGMNF*

Sequence NM_001040409

GGGGCCTGCCACGAGGCCGCAGTATAACCGCGTGGCCCGCGCGCGCGCTTCCCTCCCGGCGCAGTCACCGGCGCGGTCTATGGCTGCGACTTCTCTAATG TCTGCTTTGGCTGCCCGGCTGCTGCAGCCCGCGCACAGCTGCTCCCTTCGCCTTCGCCCTTTCCACCTCGCGGCAGTTCGGGGAATCTCTCCCTAGGTCA GGTTGGAGTGCAGTGCTGCAATCACGGCTTACTGCAGCCTTGACCTCCTGGGCTCAAGTGATCCTCCCACCTCAGCTTAAATGAAGCTGTTGTCATTTCT GGAAGGAAACTGGCCCAGCAGATCAAGCAGGAAGTGCGGCAGGAGGTAGAAGAGTGGGTGGCCTCAGGCAACAAACGGCCACACCTGAGTGTGATCCTGG TTGGCGAGAATCCTGCAAGTCACTCCTATGTCCTCAACAAAACCAGGGCAGCTGCAGTTGTGGGAATCAACAGTGAGACAATTATGAAACCAGCTTCAAT TTCAGAGGAAGAATTGTTGAATTTAATCAATAAACTGAATAATGATGATAATGTAGATGGCCTCCTTGTTCAGTTGCCTCTTCCAGAGCATATTGATGAG AGAAGGATCTGCAATGCTGTTTCTCCAGACAAGGATGTTGATGGCTTTCATGTAATTAATGTAGGACGAATGTGTTTGGATCAGTATTCCATGTTACCGG CTACTCCATGGGGTGTGTGGGAAATAATCAAGCGAACTGGCATTCCAACCCTAGGGAAGAATGTGGTTGTGGCTGGAAGGTCAAAAAACGTTGGAATGCC CATTGCAATGTTACTGCACACAGATGGGGCGCATGAACGTCCCGGAGGTGATGCCACTGTTACAATATCTCATCGATATACTCCCAAAGAGCAGTTGAAG AAACATACAATTCTTGCAGATATTGTAATATCTGCTGCAGGTATTCCAAATCTGATCACAGCAGATATGATCAAGGAAGGAGCAGCAGTCATTGATGTGG GAATAAATAGAGTTCACGATCCTGTAACTGCCAAACCCAAGTTGGTTGGAGATGTGGATTTTGAAGGAGTCAGACAAAAAGCTGGGTATATCACTCCAGT TCCTGGAGGTGTTGGCCCCATGACAGTGGCAATGCTAATGAAGAATACCATTATTGCTGCAAAAAAGGTGCTGAGGCTTGAAGAGCGAGAAGTGCTGAAG TCTAAAGAGCTTGGGGTAGCCACTAATTAACTACTGTGTCTTCTGTGTCACAAACAGCACTCCAGGCCAGCTCAAGAAGCAAAGCAGGCCAATAGAAATG CAATATTTTTAATTTATTCTACTGAAATGGTTTAAAATGATGCCTTGTATTTATTGAAAGCTTAAATGGGTGGGTGTTTCTGCACATACCTCTGCAGTAC CTCACCAGGGAGCATTCCAGTATCATGCAGGGTCCTGTGATCTAGCCAGGAGCAGCCATTAACCTAGTGATTAATATGGGAGACATTACCATATGGAGGA TGGATGCTTCACTTTGTCAAGCACCTCAGTTACACATTCGCCTTTTCTAGGATTGCATTTCCCAAGTGCTATTGCAATAACAGTTGATACTCATTTTAGG TACCAAACCTTTTGAGTTCAACTGATCAAACCAAAGGAAAAGTGTTGCTAGAGAAAATTAGGGAAAAGGTGAAAAAGAAAAAATGGTAGTAATTGAGCAG AAAAAAATTAATTTATATATGTATTGATTGGCAACCAGATTTATCTAAGTAGAACTGAATTGGCTAGGAAAAAAGAAAAACTGCATGTTAATCATTTTCC TAAGCTGTCCTTTTGAGGCTTAGTCAGTTTATTGGGAAAATGTTTAGGATTATTCCTTGCTATTAGTACTCATTTTATGTATGTTACCCTTCAGTAAGTT CTCCCCATTTTAGTTTTCTAGGACTGAAAGGATTCTTTTCTACATTATACATGTGTGTTGTCATATTTGGCTTTTGCTATATACTTTAACTTCATTGTTA AATTTTTGTATTGTATAGTTTCTTTGGTGTATCTTAAAACCTATTTTTGAAAAACAAACTTGGCTTGATAATCATTTGGGCAGCTTGGGTAAGTACGCAA CTTACTTTTCCACCAAAGAACTGTCAGCAGCTGCCTGCTTTTCTGTGATGTATGTATCCTGTTGACTTTTCCAGAAATTTTTTAAGAGTTTGAGTTACTA TTGAATTTAATCAGACTTTCTGATTAAAGGGTTTTCTTTCTTTTTTAATAAAACACATCTGTCTGGTATGGTATGAATTTCTGAAA

refseq_MTHFD2.F1 (forward primer)
refseq_MTHFD2.R1 (reverse primer)
Single exon skipping

Frame 0

GACHEAAV*PRGPRARFPPGAVTGAVYGCDFSN VCFGCPAAAARAQLLPSPSPFPPRGSSGNLSLGQ VGVQCCNHGLLQP*PPGLK*SSHLSLNEAVVIS GRKLAQQIKQEVRQEVEEWVASGNKRPHLSVIL VGENPASHSYVLNKTRAAAVVGINSETIMKPASI SEEELLNLINKLNNDDNVDGLLVQLPLPEHIDE RRICNAVSPDKDVDGFHVINVGRMCLDQYSMLP ATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMP IAMLLHTDGAHERPGGDATVTISHRYTPKEQLK KHTILADIVISAAGIPNLITADMIKEGAAVIDV GINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPV PGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLK SKELGVATN*LLCLLCHKQHSRPAQEAKQANRN AIFLIYSTEMV*NDALYLLKA*MGGCFCTYLCST SPGSIPVSCRVL*SSQEQPLT**LIWETLPYGG WMLHFVKHLSYTFAFSRIAFPKCYCNNS*YSF* VPNLLSSTDQTKGKVLLEKIREKVKKKKW**LSR KKLIYICIDWQPDLSK*N*IG*EKRKTAC*SFS *AVLLRLSQFIGKMFRIIPCY*YSFYVCYPSVS SPHFSFLGLKGFFSTLYMCVVIFGFCYIL*LHC* IFVLYSFFGVS*NLFLKNKLGLIIIWAAWVSTQ LTFPPKNCQQLPAFL*CMYPVDFSRNFLRV*VT IEFNQTF*LKGFLSFLIKHICLVWYEFLK

Frame 1

GPATRPQYNRVARARASLPAQSPARSMAATSLM SALAARLLQPAHSCSLRLRPFHLAAVRGISP*V RLECSAAITAYCSLDLLGSSDPPTSA*MKLLSFL EGNWPSRSSRKCGRR*KSGWPQATNGHT*V*SW LARILQVTPMSSTKPGQLQLWESTVRQL*NQLQ FQRKNC*I*SIN*IMMIM*MASLFSCLFQSILMR EGSAMLFLQTRMLMAFM*LM*DECVWISIPCYR LLHGVCGK*SSELAFQP*GRMWLWLEGQKTLEC PLQCYCTQMGRMNVPEVMPLLQYLIDILPKSS*R NIQFLQIL*YLLQVFQI*SQQI*SRKEQQSLMW E*IEFTIL*LPNPSWLEMWILKESDKKLGISLQ FLEVLAP*QWQC**RIPLLLQKRC*GLKSEKC*S LKSLG*PLINYCVFCVTNSTPGQLKKQSRPIEM QYF*FILLKWFKMMPCIY*KLKWVGVSAHTSAV PHQGAFQYHAGSCDLARSSH*PSD*YGRHYHMED GCFTLSSTSVTHSPFLGLHFPSAIAITVDTHFR YQTF*VQLIKPKEKCC*RKLGKR*KRKNGSN*A EKN*FIYVLIGNQIYLSRTELARKKEKLHVNHFP KLSF*GLVSLLGKCLGLFLAISTHFMYVTLQ*V LPILVF*D*KDSFLHYTCVLSYLAFAIYFNFIV KFLYCIVSLVYLKTYF*KTNLA**SFGQLG*VRN LLFHQRTVSSCLLFCDVCILLTFPEIF*EFELL LNLIRLSD*RVFFLF**NTSVWYGMNF*

Frame 2

GLPRGRSITAWPARALPSRRSHRRGLWLRLL*C LLWLPGCCSPRTAAPFAFALSTSRQFGESLPRS GWSAVLQSRLTAALTSWAQVILPPQLK*SCCHF WKETGPADQAGSAAGGRRVGGLRQQTATPECDPG WRESCKSLLCPQQNQGSCSCGNQQ*DNYETSFN FRGRIVEFNQ*TE****CRWPPCSVASSRAY** EKDLQCCFSRQGC*WLSCN*CRTNVFGSVFHVTG YSMGCVGNNQANWHSNPREECGCGWKVKKRWNA HCNVTAHRWGA*TSRR*CHCYNISSIYSQRAVE ETYNSCRYCNICCRYSKSDHSRYDQGRSSSH*CG NK*SSRSCNCQTQVGWRCGF*RSQTKSWVYHSS SWRCWPHDSGNANEEYHYCCKKGAEA*RARSAE V*RAWGSH*LTTVSSVSQTALQASSRSKAGQ*KC NIFNLFY*NGLK*CLVFIESLNGWVFLHIPLQY LTREHSSIMQGPVI*PGAAINLVINMGDITIWR MDASLCQAPQLHIRLF*DCISQVLLQ*QLILILG TKPFEFN*SNQRKSVAREN*GKGEKEKMVVIEQ KKINLYMY*LATRFI*VELNWLGKKKNCMLIIF LSCPFEA*SVYWENV*DYSLLLVLILCMLPFSKF SPF*FSRTERILFYIIHVCCHIWLLLYTLTSLL NFCIV*FLWCILKPIFEKQTWLDNHLGSLGKYA TYFSTKELSAAACFSVMYVSC*LFQKFFKSLSYY *I*SDFLIKGFSFFFNKTHLSGMV*ISE