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Sequences

Sequence NM_183078

GAGCGGAGCCTGCTTTCGCAGCGATCGCGAGCGTGTGGCGATTGCTTCTGTCTGTTATTTAGATATGGAAGCTGAGGGGATGCACAGAGGCAGCCAGAAC CTAGGTCAGGGTCTCGCTCGGTGCTGACCGCCCCCGGGGTCGAGTAGGCGATGGGGGAGCCCGGCTTCTTCGTCACAGGAGACCGCGCCGGTGGCCGGAG CTGGTGCCTGCGGCGGGTGGGGATGAGCGCCGGGTGGCTGCTGCTGGAAGATGGGTGCGAGGTGACTGTAGGACGAGGATTTGGTGTCACATACCAACTG GTATCAAAAATCTGCCCCCTGATGATTTCTCGAAACCACTGTGTTTTGAAGCAGAATCCTGAGGGCCAATGGACAATTATGGACAACAAGAGTCTAAATG GTGTTTGGCTGAACAGAGCGCGTCTGGAACCTTTAAGGGTCTATTCCATTCATCAGGGAGACTACATCCAACTTGGAGTGCCTCTGGAAAATAAGGAGAA TGCGGAGTATGAATATGAAGTTACTGAAGAAGACTGGGAGACAATATATCCTTGTCTTTCCCCAAAGAATGACCAAATGATAGAAAAAAATAAGGAATTG AGAACTAAAAGGAAATTCAGTTTGGATGAATTAGCAGGTCCTGGAGCTGAAGGCCCCTCAAATTTGAAATCCAAAATAAATAAAGTGTCTTGTGAATCTG GTCAGCCAGTGAAATCACAGGGGAAAGGTGAAGTGGCCAGTACACCCTCTGACAATTTGGATCCTAAGTTGACTGCCCTTGAGCCAAGTAAGACCACAGG GGCTCCCATTTACCCTGGCTTCCCCAAAGTCACAGAGGTTCATCATGAGCAGAAAGCCTCAAACTCTTCAGCATCTCAGAGAAGCTTACAGATGTTTAAG GTGACCATGTCCAGGATTCTGAGGCTCAAAATACAGATGCAGGAAAAACATGAAGCCGTTATGAATGTGAAAAAGCAGACCCAAAAGGGGAACTCAAAGA AAGTTGTGCAAATGGAGCAGGAACTTCAGGACTTACAGTCCCAGCTGTGTGCAGAGCAGGCTCAGCAGCAGGCAAGAGTGGAGCAACTAGAGAAGACTTT CCAGGAAGAGGAACAGCATCTTCAGGGTTTGGAGATAGCCCAAGGAGAAAAGGACCTGAAGCAACAGCTGGCCCAGGCTCTGCAGGAGCATTGGGCTCTA ATGGAAGAGCTAAATCGCAGCAAGAAGGACTTTGAAGCAATCATTCAAGCCAAGAACAAAGAATTAGAGCAGACCAAGGAAGAGAAGGAGAAGATGCAAG CACAGAAGGAAGAAGTTCTTAGCCACATGAATGATGTGCTAGAGAATGAGCTCCAATGTATTATTTGTTCAGAATACTTCATTGAGCAAAGAGATTGTTC TGAAGACCGTGCTCTAAGGGCATTTGAAAGACTGCCAGGTAGTGCGAGCCTGAGATGGTCTGGAGGATTCTCTCTAGCCGTGACTCCGCTGCTCTGAAGG TCAACTGAGAAGTCTTGTGGGACAGAGACTTGAGTTAGGAAGCCCTCAGTCACTTGCCTTCCACGGTGGCCAGCCCTGCTGCCATCATTGGCTGAAGCAC CACCAGGATTCACGGCACCCAACTGCTTCAGGGTACTTCGTAGACTCTGCCTCACTACATGTCGAAAGAGTTATTTGAGTTCTCTTCTGTTTTTTTTTAA TTTGTTGTTGTTGTTACTGTTTTGATACCTCGGAAACACCTCCGTTGACAGTTGTTTTGGATAGGTTGGGTGTACCCCATGGCTGCCTCTGAAGGCAGTG TCTATTTTGAGAGGATGGCTTACCTCTTCTTTGTGAAAATACTATCTCATTTCCTGGAAATAAAATGTAAAACCTGTCAGTTGCTCAGCTGGGCTTTGGT GTATTTACCTTCCTTCCCTTCCTGCTCCCAGACAGTCTCACAAGTAAACACCAGCAGCTCATAGATTACAACCAAGAAAGTGACTGTATCAGATGATAGA CTTCAAGTGAATGTCAGCCTAAGAGGCCAAGCTGCAGATCGTGGCAACAGATGAGCTCTGTTAACCTCCTGACTGTCGTGTTTCCTTTTTATTACAGAGG TGGGTCACTGTTTACCTTGTTTCAGGGGTGGGAGAAACTCCTTTCCTTCAGCTGGCATTTGAATGTTTCCAAATCTATTTTATCTGACGTCATGAACACA CAGGCAATGATTTTATGACAACTTGATGTGCTTTTTTCTTTATTTTTCTTTTACATTAGAAGTTTTCTCTCTTTTCTCCACCTCTCCTTTATTTTTAATT TTAATTTTAATTTTTGAAACAAGATCTGACTCTGTTGCCCAAGCTGGAGTACAGTAGTGCAATCTCAGCTCACTGCAACCTCCACCTCCCAGACTCAAGC CATCCTCCCACCTCAACCTCCCAAGTAGCTGGGACTACAGGCATATGCCATCACACCCAGCTAATTTTTGTTTTTCTGTAGAGACAGGGTTTCACCATGT TGGCCAGGCTGGTCTCAAATTCCTGACCTTAAGTGATCCACCTGCCTAGGCCTCTAAAATGCTGGGATTACAGGCGTGAGCCACCACGCCCAGCCTATAC CCCATGTATTTTCATAAAGGGTTGGAAACTATTGATTGCCAACTTTTTAAGGATGGTAGGGATTTTTTCTTTGTTGAATATACTGTGAATTTTGGGATTC TGGAGTCCTTAGTCTTCATTTCCATTCTTCTGCCCATGATTACTGATCAAAATACTGGTAGAGTCATGGAGGAAGTAAGTTAAATGTGGAAAAAATGCTT TAAAAGTACTTACTATAACCACATCATAAGAATTCTGGAAAAATAGAGAACAAAAGGAAAAGATTGTTACCACCTTGATTTAATAACTTCTTGTTTCCCC ATACATGTGTTTTGTTTATAGATTGCATGGGTATATGTCAATTTTTATATGTTCTGTGTTTAGTTTACATATTGTAATTCATTTTTAAGAGAGTACAGAC ATACACTTTTTGAGTAGGCAATATGTTCACATAGTTTGAAATTAAAGGTACAAAATGGCGTAGAATGAAATGCCTCTCACCCTTTCCCCACAGCGACTAC TTCCCACTCAGAGGCAACAAGTATTACAGTTGCTTGTATATTCTTCCAGAGATCTCTGTCTATACAAGCAAAAACACATGTGGTCTTTTTCCTTGCTTTG CACAAATGGTAAACTGTACGCTATTCTGAACCTTGCTTTTTTCAGATATATGTTGGCAACAGTTCCATGTCAGTACATAGTTTCCTTTCTTTATTACAGC TGCCTGTTTTTTCATTGTGTATTTGCACCATCATTTACTTTTCTGGCTTACTTTTGATAGAAATCTAGGTTGGTTCAAACCTTTTTACTCTTAAAACAAT GCTGCAGTTAACACCCTTGTACATATATCTTTAGAGAGAAGATATTTTAAAAAGAAATACCTTTTTAAAAATTATTTTATTATTTTTTAATAGAGACTAT GTTGCCTAGGCTGGTCTTGAAATTCTGGGCTAAAGCAATCCTACCACCTTGGCCTCCCAAAGTGCTGAGATTACAGGCATGAGCCATTGCACCTGGCCAA GAAGAGACATCTTGACTTGAGCCTGAAGACTATGTACAGAGACTGACCTCACAGACTGACCATTCCATCCCACAGCTGCTGGACATAGAGTGATTTGCAG CCCCTCCTTTCAGAGTACCACATCCCTCTCAAGTGTTCCACAACATCTAGGAAAGTGAGCTCTTAAAGACAGACTAAAAAGAGTAATAAATCAATACAAC AACATTAAAAGCCAGACTAGCGGAGTTTGAATCTTGGCCCTGCTGTTAGTAACTGTGTGAGCCTTGGGCAAGTTACTCAGCCTCCTTGTGTCTTGGTTTT GGTTTCTTCATCTCTAAGTAATTATATCTGTCATTATTGCCCTGATCACAAACAAAAGCCTGAATGTACTATGTTTAAAAGACAAACCAAAAATTAACCC AAACTTGCATTATTTGTCTGAGCTACAGAATGTTCTTTCCTTGGAGAGATATCTGATATTAAACATCATCTGCATTTTACTTGCCTAGAAAATACACGGT AACTTTTCTGCCTTGCAGCATCAAACTATAGTACAGCTGAGCCCCAGTGCTGTGCAGTCTGACTCTAATTAAAGGCACCTTCTTTACAGCAGGGCTCTGG GGAACTGGAAAAGGGGGTTTGTTTCATTATCTGGTTTTATTAAGCAGATGAATGCAGCCAGCTATATGAAGCACTTTGCAGTGAATGGCAGGTGTCCCAT ATCTGGTTATGTTAACCTAGAAAGGGCTCACTCTACCTCTAGGCATGTTTCATCCCAACAATCAGACTGTGCCAAAGCAGGGGACTTTGTCCTTTGTGGA TTGCATAGCTGGATACCCATCATCTGTTTCTCTGATTGGAAGCTGCTGTTGTACAGAAAGACCTGCATTTCCCCCTTGTCTCCAGTTCTCTCACTACTTT TTCCTCCTCTGTGAGTGACCATCCAGGCAGTCACCATAACTGCTGGAGTGTCTGGGATTGGTAGCTCTCTCCAACTGCCTGCTTGCTCTTTACAGCCTCT CTCTGTGACTGGAATCTCTCCACCTCATCGTATCTAAGGATAACCCAGAAACATGGGGTGTCCTAGGTATGTTTATCTCGACACTGAACCCCCTAGGCTT CTGATGAATCCAGTGATTAGCTAAATTTGACATAGAAAGTAAGAAGGAATGTCTACTTTGTATTGTGGTCCTAATCTAAGATCAGGAGAATCCTGGAATT GTTATCTGTCTCTTGCTCTGAGATACAGACTTGTTCATAGGTGTGGGGCCTGATTGGAACAGGATCTGCCATTGGTCACAATAGGTCAAGGGCTTGTTCT GAACCCTAGTTAGCTTCATTCAGAGAAAGAAACTTCCTACCTGGTCAGCTTTTTCAGCTTCTCCAACAAATGGGAGTTGAGGCAGTAGGAGTGTGGGGTT CTTGGGAAGACGATGGGGCCTTGTATTAAGGAGAAATAATGAACTATCTTCTTGATTCTTCCATTGAGAAAAGCATATGAATCCTAGGAAAGGGCTCAGC CTGTGATAGGCATTCAATAAATACTCCAATGCTGTCATTCCTTTGTCTACAGCATCCCCAAACAATGTACCAGTGATGGGCTACCTGAGCTCATCTAGTC GCCAAGCAGTATCTCCTGCTTGCTGCTGCTTTACTACATTCCAGATGCCCACCTCATCCAATTTCCAGAGCCACTATCTCTGCTGTCCACTTTCCTTCAG GCTCTGTGAATACTTCAACCTGCTGTGATTTGGGGCCGTTTGTACTCTGCATGTATTCAATAAATTCAATTCAGCAATAGTTA

refseq_RNF8.F2 (forward primer)
refseq_RNF8.R2 (reverse primer)
Single exon skipping

Frame 0

ERSLLSQRSRACGDCFCLLFRYGS*GDAQRQPE PRSGSRSVLTAPGVE*AMGEPGFFVTGDRAGGRS WCLRRVGMSAGWLLLEDGCEVTVGRGFGVTYQL VSKICPLMISRNHCVLKQNPEGQWTIMDNKSLN GVWLNRARLEPLRVYSIHQGDYIQLGVPLENKEN AEYEYEVTEEDWETIYPCLSPKNDQMIEKNKEL RTKRKFSLDELAGPGAEGPSNLKSKINKVSCES GQPVKSQGKGEVASTPSDNLDPKLTALEPSKTTG APIYPGFPKVTEVHHEQKASNSSASQRSLQMFK VTMSRILRLKIQMQEKHEAVMNVKKQTQKGNSK KVVQMEQELQDLQSQLCAEQAQQQARVEQLEKTF QEEEQHLQGLEIAQGEKDLKQQLAQALQEHWAL MEELNRSKKDFEAIIQAKNKELEQTKEEKEKMQ AQKEEVLSHMNDVLENELQCIICSEYFIEQRDCS EDRALRAFERLPGSASLRWSGGFSLAVTPLL*R STEKSCGTET*VRKPSVTCLPRWPALLPSLAEA PPGFTAPNCFRVLRRLCLTTCRKSYLSSLLFFFN LLLLLLF*YLGNTSVDSCFG*VGCTPWLPLKAV SILRGWLTSSL*KYYLISWK*NVKPVSCSAGLW CIYLPSLPAPRQSHK*TPAAHRLQPRK*LYQMID FK*MSA*EAKLQIVATDELC*PPDCRVSFLLQR WVTVYLVSGVGETPFLQLAFECFQIYFI*RHEH TGNDFMTT*CAFFFIFLLH*KFSLFSPPLLYF*F *F*FLKQDLTLLPKLEYSSAISAHCNLHLPDSS HPPTSTSQVAGTTGICHHTQLIFVFL*RQGFTM LARLVSNS*P*VIHLPRPLKCWDYRREPPRPAYT PCIFIKGWKLLIANFLRMVGIFSLLNIL*ILGF WSP*SSFPFFCP*LLIKILVESWRK*VKCGKNA LKVLTITTS*EFWKNREQKEKIVTTLI**LLVSP YMCFVYRLHGYMSIFICSVFSLHIVIHF*ESTD IHFLSRQYVHIV*N*RYKMA*NEMPLTLSPQRL LPTQRQQVLQLLVYSSRDLCLYKQKHMWSFSLLC TNGKLYAILNLAFFRYMLATVPCQYIVSFLYYS CLFFHCVFAPSFTFLAYF**KSRLVQTFLLLKQ CCS*HPCTYIFREKIF*KEIPF*KLFYYFLIETM LPRLVLKFWAKAILPPWPPKVLRLQA*AIAPGQ EETS*LEPEDYVQRLTSQTDHSIPQLLDIE*FA APPFRVPHPSQVFHNI*ESELLKTD*KE**INTT TLKARLAEFESWPCC**LCEPWASYSASLCLGF GFFISK*LYLSLLP*SQTKA*MYYV*KTNQKLT QTCIICLSYRMFFPWRDI*Y*TSSAFYLPRKYTV TFLPCSIKL*YS*APVLCSLTLIKGTFFTAGLW GTGKGGLFHYLVLLSR*MQPAI*STLQ*MAGVP YLVMLT*KGLTLPLGMFHPNNQTVPKQGTLSFVD CIAGYPSSVSLIGSCCCTERPAFPPCLQFSHYF FLLCE*PSRQSP*LLECLGLVALSNCLLALYSL SL*LESLHLIVSKDNPETWGVLGMFISTLNPLGF **IQ*LAKFDIESKKECLLCIVVLI*DQENPGI VICLLL*DTDLFIGVGPDWNRICHWSQ*VKGLF *TLVSFIQRKKLPTWSAFSASPTNGS*GSRSVGF LGRRWGLVLRRNNELSS*FFH*EKHMNPRKGLS L**AFNKYSNAVIPLSTASPNNVPVMGYLSSSS RQAVSPACCCFTTFQMPTSSNFQSHYLCCPLSFR LCEYFNLL*FGAVCTLHVFNKFNSAIV

Frame 1

SGACFRSDRERVAIASVCYLDMEAEGMHRGSQN LGQGLARC*PPPGSSRRWGSPASSSQETAPVAG AGACGGWG*APGGCCWKMGAR*L*DEDLVSHTNW YQKSAP**FLETTVF*SRILRANGQLWTTRV*M VFG*TERVWNL*GSIPFIRETTSNLECLWKIRR MRSMNMKLLKKTGRQYILVFPQRMTK**KKIRN* ELKGNSVWMN*QVLELKAPQI*NPK*IKCLVNL VSQ*NHRGKVKWPVHPLTIWILS*LPLSQVRPQ GLPFTLASPKSQRFIMSRKPQTLQHLREAYRCLR *PCPGF*GSKYRCRKNMKPL*M*KSRPKRGTQR KLCKWSRNFRTYSPSCVQSRLSSRQEWSN*RRL SRKRNSIFRVWR*PKEKRT*SNSWPRLCRSIGL* WKS*IAARRTLKQSFKPRTKN*SRPRKRRRRCK HRRKKFLAT*MMC*RMSSNVLFVQNTSLSKEIV LKTVL*GHLKDCQVVRA*DGLEDSL*P*LRCSEG QLRSLVGQRLELGSPQSLAFHGGQPCCHHWLKH HQDSRHPTASGYFVDSASLHVERVI*VLFCFFL ICCCCYCFDTSETPPLTVVLDRLGVPHGCL*RQC LF*EDGLPLLCENTISFPGNKM*NLSVAQLGFG VFTFLPFLLPDSLTSKHQQLIDYNQESDCIR** TSSECQPKRPSCRSWQQMSSVNLLTVVFPFYYRG GSLFTLFQGWEKLLSFSWHLNVSKSILSDVMNT QAMIL*QLDVLFSLFFFYIRSFLSFLHLSFIFN FNFNF*NKI*LCCPSWSTVVQSQLTATSTSQTQA ILPPQPPK*LGLQAYAITPS*FLFFCRDRVSPC WPGWSQIPDLK*STCLGL*NAGITGVSHHAQPI PHVFS*RVGNY*LPTF*GW*GFFLC*IYCEFWDS GVLSLHFHSSAHDY*SKYW*SHGGSKLNVEKML *KYLL*PHHKNSGKIENKRKRLLPP*FNNFLFP HTCVLFIDCMGICQFLYVLCLVYIL*FIFKRVQT YTF*VGNMFT*FEIKGTKWRRMKCLSPFPHSDY FPLRGNKYYSCLYILPEISVYTSKNTCGLFPCF AQMVNCTLF*TLLFSDICWQQFHVST*FPFFITA ACFFIVYLHHHLLFWLTFDRNLGWFKPFYS*NN AAVNTLVHISLERRYFKKKYLFKNYFIIF**RL CCLGWS*NSGLKQSYHLGLPKC*DYRHEPLHLAK KRHLDLSLKTMYRD*PHRLTIPSHSCWT*SDLQ PLLSEYHIPLKCSTTSRKVSS*RQTKKSNKSIQ QH*KPD*RSLNLGPAVSNCVSLGQVTQPPCVLVL VSSSLSNYICHYCPDHKQKPECTMFKRQTKN*P KLALFV*ATECSFLGEISDIKHHLHFTCLENTR *LFCLAASNYSTAEPQCCAV*L*LKAPSLQQGSG ELEKGVCFIIWFY*ADECSQLYEALCSEWQVSH IWLC*PRKGSLYL*ACFIPTIRLCQSRGLCPLW IA*LDTHHLFL*LEAAVVQKDLHFPLVSSSLTTF SSSVSDHPGSHHNCWSVWDW*LSPTACLLFTAS LCDWNLSTSSYLRITQKHGVS*VCLSRH*TP*A SDESSD*LNLT*KVRRNVYFVLWS*SKIRRILEL LSVSCSEIQTCS*VWGLIGTGSAIGHNRSRACS EP*LASFRERNFLPGQLFQLLQQMGVEAVGVWG SWEDDGALY*GEIMNYLLDSSIEKSI*ILGKGSA CDRHSINTPMLSFLCLQHPQTMYQ*WAT*AHLV AKQYLLLAAALLHSRCPPHPISRATISAVHFPS GSVNTSTCCDLGPFVLCMYSINSIQQ*L

Frame 2

AEPAFAAIASVWRLLLSVI*IWKLRGCTEAART *VRVSLGADRPRGRVGDGGARLLRHRRPRRWPE LVPAAGGDERRVAAAGRWVRGDCRTRIWCHIPT GIKNLPPDDFSKPLCFEAES*GPMDNYGQQESKW CLAEQSASGTFKGLFHSSGRLHPTWSASGK*GE CGV*I*SY*RRLGDNISLSFPKE*PNDRKK*GI EN*KEIQFG*ISRSWS*RPLKFEIQNK*SVL*IW SASEITGER*SGQYTL*QFGS*VDCP*AK*DHR GSHLPWLPQSHRGSS*AESLKLFSISEKLTDV* GDHVQDSEAQNTDAGKT*SRYECEKADPKGELKE SCANGAGTSGLTVPAVCRAGSAAGKSGATREDF PGRGTASSGFGDSPRRKGPEATAGPGSAGALGS NGRAKSQQEGL*SNHSSQEQRIRADQGREGEDAS TEGRSS*PHE*CARE*APMYYLFRILH*AKRLF *RPCSKGI*KTAR*CEPEMVWRILSSRDSAALK VN*EVLWDRDLS*EALSHLPSTVASPAAIIG*ST TRIHGTQLLQGTS*TLPHYMSKELFEFSSVFF* FVVVVTVLIPRKHLR*QLFWIGWVYPMAASEGS VYFERMAYLFFVKILSHFLEIKCKTCQLLSWALV YLPSFPSCSQTVSQVNTSSS*ITTKKVTVSDDR LQVNVSLRGQAADRGNR*ALLTS*LSCFLFITE VGHCLPCFRGGRNSFPSAGI*MFPNLFYLTS*TH RQ*FYDNLMCFFLYFSFTLEVFSLFSTSPLFLI LILIFETRSDSVAQAGVQ*CNLSSLQPPPPRLK PSSHLNLPSSWDYRHMPSHPANFCFSVETGFHHV GQAGLKFLTLSDPPA*ASKMLGLQA*ATTPSLY PMYFHKGLETIDCQLFKDGRDFFFVEYTVNFGI LESLVFISILLPMITDQNTGRVMEEVS*MWKKCF KSTYYNHIIRILEK*RTKGKDCYHLDLITSCFP IHVFCL*IAWVYVNFYMFCV*FTYCNSFLREYR HTLFE*AICSHSLKLKVQNGVE*NASHPFPTATT SHSEATSITVACIFFQRSLSIQAKTHVVFFLAL HKW*TVRYSEPCFFQIYVGNSSMSVHSFLSLLQ LPVFSLCICTIIYFSGLLLIEI*VGSNLFTLKTM LQLTPLYIYL*REDILKRNTFLKIILLFFNRDY VA*AGLEILG*SNPTTLASQSAEITGMSHCTWP RRDILT*A*RLCTETDLTD*PFHPTAAGHRVICS PSFQSTTSLSSVPQHLGK*ALKDRLKRVINQYN NIKSQTSGV*ILALLLVTV*ALGKLLSLLVSWF WFLHL*VIISVIIALITNKSLNVLCLKDKPKINP NLHYLSELQNVLSLERYLILNIICILLA*KIHG NFSALQHQTIVQLSPSAVQSDSN*RHLLYSRAL GNWKRGFVSLSGFIKQMNAASYMKHFAVNGRCPI SGYVNLERAHSTSRHVSSQQSDCAKAGDFVLCG LHSWIPIICFSDWKLLLYRKTCISPLSPVLSLL FPPL*VTIQAVTITAGVSGIGSSLQLPACSLQPL SVTGISPPHRI*G*PRNMGCPRYVYLDTEPPRL LMNPVIS*I*HRK*EGMSTLYCGPNLRSGESWN CYLSLALRYRLVHRCGA*LEQDLPLVTIGQGLVL NPS*LHSEKETSYLVSFFSFSNKWELRQ*ECGV LGKTMGPCIKEK**TIFLILPLRKAYES*ERAQ PVIGIQ*ILQCCHSFVYSIPKQCTSDGLPELI*S PSSISCLLLLYYIPDAHLIQFPEPLSLLSTFLQ AL*ILQPAVIWGRLYSACIQ*IQFSNS

Sequence NM_003958

GAGCGGAGCCTGCTTTCGCAGCGATCGCGAGCGTGTGGCGATTGCTTCTGTCTGTTATTTAGATATGGAAGCTGAGGGGATGCACAGAGGCAGCCAGAAC CTAGGTCAGGGTCTCGCTCGGTGCTGACCGCCCCCGGGGTCGAGTAGGCGATGGGGGAGCCCGGCTTCTTCGTCACAGGAGACCGCGCCGGTGGCCGGAG CTGGTGCCTGCGGCGGGTGGGGATGAGCGCCGGGTGGCTGCTGCTGGAAGATGGGTGCGAGGTGACTGTAGGACGAGGATTTGGTGTCACATACCAACTG GTATCAAAAATCTGCCCCCTGATGATTTCTCGAAACCACTGTGTTTTGAAGCAGAATCCTGAGGGCCAATGGACAATTATGGACAACAAGAGTCTAAATG GTGTTTGGCTGAACAGAGCGCGTCTGGAACCTTTAAGGGTCTATTCCATTCATCAGGGAGACTACATCCAACTTGGAGTGCCTCTGGAAAATAAGGAGAA TGCGGAGTATGAATATGAAGTTACTGAAGAAGACTGGGAGACAATATATCCTTGTCTTTCCCCAAAGAATGACCAAATGATAGAAAAAAATAAGGAATTG AGAACTAAAAGGAAATTCAGTTTGGATGAATTAGCAGGTCCTGGAGCTGAAGGCCCCTCAAATTTGAAATCCAAAATAAATAAAGTGTCTTGTGAATCTG GTCAGCCAGTGAAATCACAGGGGAAAGGTGAAGTGGCCAGTACACCCTCTGACAATTTGGATCCTAAGTTGACTGCCCTTGAGCCAAGTAAGACCACAGG GGCTCCCATTTACCCTGGCTTCCCCAAAGTCACAGAGGTTCATCATGAGCAGAAAGCCTCAAACTCTTCAGCATCTCAGAGAAGCTTACAGATGTTTAAG GTGACCATGTCCAGGATTCTGAGGCTCAAAATACAGATGCAGGAAAAACATGAAGCCGTTATGAATGTGAAAAAGCAGACCCAAAAGGGGAACTCAAAGA AAGTTGTGCAAATGGAGCAGGAACTTCAGGACTTACAGTCCCAGCTGTGTGCAGAGCAGGCTCAGCAGCAGGCAAGAGTGGAGCAACTAGAGAAGACTTT CCAGGAAGAGGAACAGCATCTTCAGGGTTTGGAGATAGCCCAAGGAGAAAAGGACCTGAAGCAACAGCTGGCCCAGGCTCTGCAGGAGCATTGGGCTCTA ATGGAAGAGCTAAATCGCAGCAAGAAGGACTTTGAAGCAATCATTCAAGCCAAGAACAAAGAATTAGAGCAGACCAAGGAAGAGAAGGAGAAGATGCAAG CACAGAAGGAAGAAGTTCTTAGCCACATGAATGATGTGCTAGAGAATGAGCTCCAATGTATTATTTGTTCAGAATACTTCATTGAGGCTGTCACCTTGAA CTGTGCCCACAGTTTCTGCTCCTACTGTATCAATGAATGGATGAAGCGGAAGATAGAATGCCCCATTTGTCGGAAGGACATTAAGTCCAAAACGTACTCT TTGGTTCTGGACAATTGCATTAATAAGATGGTAAATAATCTGAGCTCAGAAGTGAAAGAACGACGAATTGTTCTCATTAGGGAACGAAAAGCAAAGAGAT TGTTCTGAAGACCGTGCTCTAAGGGCATTTGAAAGACTGCCAGGTAGTGCGAGCCTGAGATGGTCTGGAGGATTCTCTCTAGCCGTGACTCCGCTGCTCT GAAGGTCAACTGAGAAGTCTTGTGGGACAGAGACTTGAGTTAGGAAGCCCTCAGTCACTTGCCTTCCACGGTGGCCAGCCCTGCTGCCATCATTGGCTGA AGCACCACCAGGATTCACGGCACCCAACTGCTTCAGGGTACTTCGTAGACTCTGCCTCACTACATGTCGAAAGAGTTATTTGAGTTCTCTTCTGTTTTTT TTTAATTTGTTGTTGTTGTTACTGTTTTGATACCTCGGAAACACCTCCGTTGACAGTTGTTTTGGATAGGTTGGGTGTACCCCATGGCTGCCTCTGAAGG CAGTGTCTATTTTGAGAGGATGGCTTACCTCTTCTTTGTGAAAATACTATCTCATTTCCTGGAAATAAAATGTAAAACCTGTCAGTTGCTCAGCTGGGCT TTGGTGTATTTACCTTCCTTCCCTTCCTGCTCCCAGACAGTCTCACAAGTAAACACCAGCAGCTCATAGATTACAACCAAGAAAGTGACTGTATCAGATG ATAGACTTCAAGTGAATGTCAGCCTAAGAGGCCAAGCTGCAGATCGTGGCAACAGATGAGCTCTGTTAACCTCCTGACTGTCGTGTTTCCTTTTTATTAC AGAGGTGGGTCACTGTTTACCTTGTTTCAGGGGTGGGAGAAACTCCTTTCCTTCAGCTGGCATTTGAATGTTTCCAAATCTATTTTATCTGACGTCATGA ACACACAGGCAATGATTTTATGACAACTTGATGTGCTTTTTTCTTTATTTTTCTTTTACATTAGAAGTTTTCTCTCTTTTCTCCACCTCTCCTTTATTTT TAATTTTAATTTTAATTTTTGAAACAAGATCTGACTCTGTTGCCCAAGCTGGAGTACAGTAGTGCAATCTCAGCTCACTGCAACCTCCACCTCCCAGACT CAAGCCATCCTCCCACCTCAACCTCCCAAGTAGCTGGGACTACAGGCATATGCCATCACACCCAGCTAATTTTTGTTTTTCTGTAGAGACAGGGTTTCAC CATGTTGGCCAGGCTGGTCTCAAATTCCTGACCTTAAGTGATCCACCTGCCTAGGCCTCTAAAATGCTGGGATTACAGGCGTGAGCCACCACGCCCAGCC TATACCCCATGTATTTTCATAAAGGGTTGGAAACTATTGATTGCCAACTTTTTAAGGATGGTAGGGATTTTTTCTTTGTTGAATATACTGTGAATTTTGG GATTCTGGAGTCCTTAGTCTTCATTTCCATTCTTCTGCCCATGATTACTGATCAAAATACTGGTAGAGTCATGGAGGAAGTAAGTTAAATGTGGAAAAAA TGCTTTAAAAGTACTTACTATAACCACATCATAAGAATTCTGGAAAAATAGAGAACAAAAGGAAAAGATTGTTACCACCTTGATTTAATAACTTCTTGTT TCCCCATACATGTGTTTTGTTTATAGATTGCATGGGTATATGTCAATTTTTATATGTTCTGTGTTTAGTTTACATATTGTAATTCATTTTTAAGAGAGTA CAGACATACACTTTTTGAGTAGGCAATATGTTCACATAGTTTGAAATTAAAGGTACAAAATGGCGTAGAATGAAATGCCTCTCACCCTTTCCCCACAGCG ACTACTTCCCACTCAGAGGCAACAAGTATTACAGTTGCTTGTATATTCTTCCAGAGATCTCTGTCTATACAAGCAAAAACACATGTGGTCTTTTTCCTTG CTTTGCACAAATGGTAAACTGTACGCTATTCTGAACCTTGCTTTTTTCAGATATATGTTGGCAACAGTTCCATGTCAGTACATAGTTTCCTTTCTTTATT ACAGCTGCCTGTTTTTTCATTGTGTATTTGCACCATCATTTACTTTTCTGGCTTACTTTTGATAGAAATCTAGGTTGGTTCAAACCTTTTTACTCTTAAA ACAATGCTGCAGTTAACACCCTTGTACATATATCTTTAGAGAGAAGATATTTTAAAAAGAAATACCTTTTTAAAAATTATTTTATTATTTTTTAATAGAG ACTATGTTGCCTAGGCTGGTCTTGAAATTCTGGGCTAAAGCAATCCTACCACCTTGGCCTCCCAAAGTGCTGAGATTACAGGCATGAGCCATTGCACCTG GCCAAGAAGAGACATCTTGACTTGAGCCTGAAGACTATGTACAGAGACTGACCTCACAGACTGACCATTCCATCCCACAGCTGCTGGACATAGAGTGATT TGCAGCCCCTCCTTTCAGAGTACCACATCCCTCTCAAGTGTTCCACAACATCTAGGAAAGTGAGCTCTTAAAGACAGACTAAAAAGAGTAATAAATCAAT ACAACAACATTAAAAGCCAGACTAGCGGAGTTTGAATCTTGGCCCTGCTGTTAGTAACTGTGTGAGCCTTGGGCAAGTTACTCAGCCTCCTTGTGTCTTG GTTTTGGTTTCTTCATCTCTAAGTAATTATATCTGTCATTATTGCCCTGATCACAAACAAAAGCCTGAATGTACTATGTTTAAAAGACAAACCAAAAATT AACCCAAACTTGCATTATTTGTCTGAGCTACAGAATGTTCTTTCCTTGGAGAGATATCTGATATTAAACATCATCTGCATTTTACTTGCCTAGAAAATAC ACGGTAACTTTTCTGCCTTGCAGCATCAAACTATAGTACAGCTGAGCCCCAGTGCTGTGCAGTCTGACTCTAATTAAAGGCACCTTCTTTACAGCAGGGC TCTGGGGAACTGGAAAAGGGGGTTTGTTTCATTATCTGGTTTTATTAAGCAGATGAATGCAGCCAGCTATATGAAGCACTTTGCAGTGAATGGCAGGTGT CCCATATCTGGTTATGTTAACCTAGAAAGGGCTCACTCTACCTCTAGGCATGTTTCATCCCAACAATCAGACTGTGCCAAAGCAGGGGACTTTGTCCTTT GTGGATTGCATAGCTGGATACCCATCATCTGTTTCTCTGATTGGAAGCTGCTGTTGTACAGAAAGACCTGCATTTCCCCCTTGTCTCCAGTTCTCTCACT ACTTTTTCCTCCTCTGTGAGTGACCATCCAGGCAGTCACCATAACTGCTGGAGTGTCTGGGATTGGTAGCTCTCTCCAACTGCCTGCTTGCTCTTTACAG CCTCTCTCTGTGACTGGAATCTCTCCACCTCATCGTATCTAAGGATAACCCAGAAACATGGGGTGTCCTAGGTATGTTTATCTCGACACTGAACCCCCTA GGCTTCTGATGAATCCAGTGATTAGCTAAATTTGACATAGAAAGTAAGAAGGAATGTCTACTTTGTATTGTGGTCCTAATCTAAGATCAGGAGAATCCTG GAATTGTTATCTGTCTCTTGCTCTGAGATACAGACTTGTTCATAGGTGTGGGGCCTGATTGGAACAGGATCTGCCATTGGTCACAATAGGTCAAGGGCTT GTTCTGAACCCTAGTTAGCTTCATTCAGAGAAAGAAACTTCCTACCTGGTCAGCTTTTTCAGCTTCTCCAACAAATGGGAGTTGAGGCAGTAGGAGTGTG GGGTTCTTGGGAAGACGATGGGGCCTTGTATTAAGGAGAAATAATGAACTATCTTCTTGATTCTTCCATTGAGAAAAGCATATGAATCCTAGGAAAGGGC TCAGCCTGTGATAGGCATTCAATAAATACTCCAATGCTGTCATTCCTTTGTCTACAGCATCCCCAAACAATGTACCAGTGATGGGCTACCTGAGCTCATC TAGTCGCCAAGCAGTATCTCCTGCTTGCTGCTGCTTTACTACATTCCAGATGCCCACCTCATCCAATTTCCAGAGCCACTATCTCTGCTGTCCACTTTCC TTCAGGCTCTGTGAATACTTCAACCTGCTGTGATTTGGGGCCGTTTGTACTCTGCATGTATTCAATAAATTCAATTCAGCAATAGTTA

refseq_RNF8.F2 (forward primer)
refseq_RNF8.R2 (reverse primer)
Single exon skipping

Frame 0

ERSLLSQRSRACGDCFCLLFRYGS*GDAQRQPE PRSGSRSVLTAPGVE*AMGEPGFFVTGDRAGGRS WCLRRVGMSAGWLLLEDGCEVTVGRGFGVTYQL VSKICPLMISRNHCVLKQNPEGQWTIMDNKSLN GVWLNRARLEPLRVYSIHQGDYIQLGVPLENKEN AEYEYEVTEEDWETIYPCLSPKNDQMIEKNKEL RTKRKFSLDELAGPGAEGPSNLKSKINKVSCES GQPVKSQGKGEVASTPSDNLDPKLTALEPSKTTG APIYPGFPKVTEVHHEQKASNSSASQRSLQMFK VTMSRILRLKIQMQEKHEAVMNVKKQTQKGNSK KVVQMEQELQDLQSQLCAEQAQQQARVEQLEKTF QEEEQHLQGLEIAQGEKDLKQQLAQALQEHWAL MEELNRSKKDFEAIIQAKNKELEQTKEEKEKMQ AQKEEVLSHMNDVLENELQCIICSEYFIEAVTLN CAHSFCSYCINEWMKRKIECPICRKDIKSKTYS LVLDNCINKMVNNLSSEVKERRIVLIRERKAKR LF*RPCSKGI*KTAR*CEPEMVWRILSSRDSAAL KVN*EVLWDRDLS*EALSHLPSTVASPAAIIG* STTRIHGTQLLQGTS*TLPHYMSKELFEFSSVF F*FVVVVTVLIPRKHLR*QLFWIGWVYPMAASEG SVYFERMAYLFFVKILSHFLEIKCKTCQLLSWA LVYLPSFPSCSQTVSQVNTSSS*ITTKKVTVSD DRLQVNVSLRGQAADRGNR*ALLTS*LSCFLFIT EVGHCLPCFRGGRNSFPSAGI*MFPNLFYLTS* THRQ*FYDNLMCFFLYFSFTLEVFSLFSTSPLF LILILIFETRSDSVAQAGVQ*CNLSSLQPPPPRL KPSSHLNLPSSWDYRHMPSHPANFCFSVETGFH HVGQAGLKFLTLSDPPA*ASKMLGLQA*ATTPS LYPMYFHKGLETIDCQLFKDGRDFFFVEYTVNFG ILESLVFISILLPMITDQNTGRVMEEVS*MWKK CFKSTYYNHIIRILEK*RTKGKDCYHLDLITSC FPIHVFCL*IAWVYVNFYMFCV*FTYCNSFLREY RHTLFE*AICSHSLKLKVQNGVE*NASHPFPTA TTSHSEATSITVACIFFQRSLSIQAKTHVVFFL ALHKW*TVRYSEPCFFQIYVGNSSMSVHSFLSLL QLPVFSLCICTIIYFSGLLLIEI*VGSNLFTLK TMLQLTPLYIYL*REDILKRNTFLKIILLFFNR DYVA*AGLEILG*SNPTTLASQSAEITGMSHCTW PRRDILT*A*RLCTETDLTD*PFHPTAAGHRVI CSPSFQSTTSLSSVPQHLGK*ALKDRLKRVINQ YNNIKSQTSGV*ILALLLVTV*ALGKLLSLLVSW FWFLHL*VIISVIIALITNKSLNVLCLKDKPKI NPNLHYLSELQNVLSLERYLILNIICILLA*KI HGNFSALQHQTIVQLSPSAVQSDSN*RHLLYSRA LGNWKRGFVSLSGFIKQMNAASYMKHFAVNGRC PISGYVNLERAHSTSRHVSSQQSDCAKAGDFVL CGLHSWIPIICFSDWKLLLYRKTCISPLSPVLSL LFPPL*VTIQAVTITAGVSGIGSSLQLPACSLQ PLSVTGISPPHRI*G*PRNMGCPRYVYLDTEPP RLLMNPVIS*I*HRK*EGMSTLYCGPNLRSGESW NCYLSLALRYRLVHRCGA*LEQDLPLVTIGQGL VLNPS*LHSEKETSYLVSFFSFSNKWELRQ*EC GVLGKTMGPCIKEK**TIFLILPLRKAYES*ERA QPVIGIQ*ILQCCHSFVYSIPKQCTSDGLPELI *SPSSISCLLLLYYIPDAHLIQFPEPLSLLSTF LQAL*ILQPAVIWGRLYSACIQ*IQFSNS

Frame 1

SGACFRSDRERVAIASVCYLDMEAEGMHRGSQN LGQGLARC*PPPGSSRRWGSPASSSQETAPVAG AGACGGWG*APGGCCWKMGAR*L*DEDLVSHTNW YQKSAP**FLETTVF*SRILRANGQLWTTRV*M VFG*TERVWNL*GSIPFIRETTSNLECLWKIRR MRSMNMKLLKKTGRQYILVFPQRMTK**KKIRN* ELKGNSVWMN*QVLELKAPQI*NPK*IKCLVNL VSQ*NHRGKVKWPVHPLTIWILS*LPLSQVRPQ GLPFTLASPKSQRFIMSRKPQTLQHLREAYRCLR *PCPGF*GSKYRCRKNMKPL*M*KSRPKRGTQR KLCKWSRNFRTYSPSCVQSRLSSRQEWSN*RRL SRKRNSIFRVWR*PKEKRT*SNSWPRLCRSIGL* WKS*IAARRTLKQSFKPRTKN*SRPRKRRRRCK HRRKKFLAT*MMC*RMSSNVLFVQNTSLRLSP* TVPTVSAPTVSMNG*SGR*NAPFVGRTLSPKRTL WFWTIALIRW*II*AQK*KNDELFSLGNEKQRD CSEDRALRAFERLPGSASLRWSGGFSLAVTPLL *RSTEKSCGTET*VRKPSVTCLPRWPALLPSLAE APPGFTAPNCFRVLRRLCLTTCRKSYLSSLLFF FNLLLLLLF*YLGNTSVDSCFG*VGCTPWLPLK AVSILRGWLTSSL*KYYLISWK*NVKPVSCSAGL WCIYLPSLPAPRQSHK*TPAAHRLQPRK*LYQM IDFK*MSA*EAKLQIVATDELC*PPDCRVSFLL QRWVTVYLVSGVGETPFLQLAFECFQIYFI*RHE HTGNDFMTT*CAFFFIFLLH*KFSLFSPPLLYF *F*F*FLKQDLTLLPKLEYSSAISAHCNLHLPD SSHPPTSTSQVAGTTGICHHTQLIFVFL*RQGFT MLARLVSNS*P*VIHLPRPLKCWDYRREPPRPA YTPCIFIKGWKLLIANFLRMVGIFSLLNIL*IL GFWSP*SSFPFFCP*LLIKILVESWRK*VKCGKN ALKVLTITTS*EFWKNREQKEKIVTTLI**LLV SPYMCFVYRLHGYMSIFICSVFSLHIVIHF*ES TDIHFLSRQYVHIV*N*RYKMA*NEMPLTLSPQR LLPTQRQQVLQLLVYSSRDLCLYKQKHMWSFSL LCTNGKLYAILNLAFFRYMLATVPCQYIVSFLY YSCLFFHCVFAPSFTFLAYF**KSRLVQTFLLLK QCCS*HPCTYIFREKIF*KEIPF*KLFYYFLIE TMLPRLVLKFWAKAILPPWPPKVLRLQA*AIAP GQEETS*LEPEDYVQRLTSQTDHSIPQLLDIE*F AAPPFRVPHPSQVFHNI*ESELLKTD*KE**IN TTTLKARLAEFESWPCC**LCEPWASYSASLCL GFGFFISK*LYLSLLP*SQTKA*MYYV*KTNQKL TQTCIICLSYRMFFPWRDI*Y*TSSAFYLPRKY TVTFLPCSIKL*YS*APVLCSLTLIKGTFFTAG LWGTGKGGLFHYLVLLSR*MQPAI*STLQ*MAGV PYLVMLT*KGLTLPLGMFHPNNQTVPKQGTLSF VDCIAGYPSSVSLIGSCCCTERPAFPPCLQFSH YFFLLCE*PSRQSP*LLECLGLVALSNCLLALYS LSL*LESLHLIVSKDNPETWGVLGMFISTLNPL GF**IQ*LAKFDIESKKECLLCIVVLI*DQENP GIVICLLL*DTDLFIGVGPDWNRICHWSQ*VKGL F*TLVSFIQRKKLPTWSAFSASPTNGS*GSRSV GFLGRRWGLVLRRNNELSS*FFH*EKHMNPRKG LSL**AFNKYSNAVIPLSTASPNNVPVMGYLSSS SRQAVSPACCCFTTFQMPTSSNFQSHYLCCPLS FRLCEYFNLL*FGAVCTLHVFNKFNSAIV

Frame 2

AEPAFAAIASVWRLLLSVI*IWKLRGCTEAART *VRVSLGADRPRGRVGDGGARLLRHRRPRRWPE LVPAAGGDERRVAAAGRWVRGDCRTRIWCHIPT GIKNLPPDDFSKPLCFEAES*GPMDNYGQQESKW CLAEQSASGTFKGLFHSSGRLHPTWSASGK*GE CGV*I*SY*RRLGDNISLSFPKE*PNDRKK*GI EN*KEIQFG*ISRSWS*RPLKFEIQNK*SVL*IW SASEITGER*SGQYTL*QFGS*VDCP*AK*DHR GSHLPWLPQSHRGSS*AESLKLFSISEKLTDV* GDHVQDSEAQNTDAGKT*SRYECEKADPKGELKE SCANGAGTSGLTVPAVCRAGSAAGKSGATREDF PGRGTASSGFGDSPRRKGPEATAGPGSAGALGS NGRAKSQQEGL*SNHSSQEQRIRADQGREGEDAS TEGRSS*PHE*CARE*APMYYLFRILH*GCHLE LCPQFLLLLYQ*MDEAEDRMPHLSEGH*VQNVL FGSGQLH**DGK*SELRSERTTNCSH*GTKSKEI VLKTVL*GHLKDCQVVRA*DGLEDSL*P*LRCS EGQLRSLVGQRLELGSPQSLAFHGGQPCCHHWL KHHQDSRHPTASGYFVDSASLHVERVI*VLFCFF LICCCCYCFDTSETPPLTVVLDRLGVPHGCL*R QCLF*EDGLPLLCENTISFPGNKM*NLSVAQLG FGVFTFLPFLLPDSLTSKHQQLIDYNQESDCIR* *TSSECQPKRPSCRSWQQMSSVNLLTVVFPFYY RGGSLFTLFQGWEKLLSFSWHLNVSKSILSDVM NTQAMIL*QLDVLFSLFFFYIRSFLSFLHLSFIF NFNFNF*NKI*LCCPSWSTVVQSQLTATSTSQT QAILPPQPPK*LGLQAYAITPS*FLFFCRDRVS PCWPGWSQIPDLK*STCLGL*NAGITGVSHHAQP IPHVFS*RVGNY*LPTF*GW*GFFLC*IYCEFW DSGVLSLHFHSSAHDY*SKYW*SHGGSKLNVEK ML*KYLL*PHHKNSGKIENKRKRLLPP*FNNFLF PHTCVLFIDCMGICQFLYVLCLVYIL*FIFKRV QTYTF*VGNMFT*FEIKGTKWRRMKCLSPFPHS DYFPLRGNKYYSCLYILPEISVYTSKNTCGLFPC FAQMVNCTLF*TLLFSDICWQQFHVST*FPFFI TAACFFIVYLHHHLLFWLTFDRNLGWFKPFYS* NNAAVNTLVHISLERRYFKKKYLFKNYFIIF**R LCCLGWS*NSGLKQSYHLGLPKC*DYRHEPLHL AKKRHLDLSLKTMYRD*PHRLTIPSHSCWT*SD LQPLLSEYHIPLKCSTTSRKVSS*RQTKKSNKSI QQH*KPD*RSLNLGPAVSNCVSLGQVTQPPCVL VLVSSSLSNYICHYCPDHKQKPECTMFKRQTKN *PKLALFV*ATECSFLGEISDIKHHLHFTCLENT R*LFCLAASNYSTAEPQCCAV*L*LKAPSLQQG SGELEKGVCFIIWFY*ADECSQLYEALCSEWQV SHIWLC*PRKGSLYL*ACFIPTIRLCQSRGLCPL WIA*LDTHHLFL*LEAAVVQKDLHFPLVSSSLT TFSSSVSDHPGSHHNCWSVWDW*LSPTACLLFT ASLCDWNLSTSSYLRITQKHGVS*VCLSRH*TP* ASDESSD*LNLT*KVRRNVYFVLWS*SKIRRIL ELLSVSCSEIQTCS*VWGLIGTGSAIGHNRSRA CSEP*LASFRERNFLPGQLFQLLQQMGVEAVGVW GSWEDDGALY*GEIMNYLLDSSIEKSI*ILGKG SACDRHSINTPMLSFLCLQHPQTMYQ*WAT*AH LVAKQYLLLAAALLHSRCPPHPISRATISAVHFP SGSVNTSTCCDLGPFVLCMYSINSIQQ*L