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Sequences

Sequence NM_201539

GGGTCTGCGGGAAGCCCGAGCCGGGGCGGGCCCGAGTCAAAGGCAAGTGAAGGTGGAAGCGGCCGCGGCGGCAGCAGGCCACCATGGCGGAGCTGCAGGA
GGTGCAGATCACAGAGGAGAAGCCACTGTTGCCAGGACAGACGCCTGAGGCGGCCAAGGAGGCTGAGTTAGCTGCCCGAATCCTCCTGGACCAGGGACAG
ACTCACTCTGTGGAGACACCATACGGCTCTGTCACTTTCACTGTCTATGGCACCCCCAAACCCAAACGCCCAGCGATCCTTACCTACCACGATGTGGGAC
TCAACTATAAATCTTGCTTCCAGCCACTGTTTCAGTTCGAGGACATGCAGGAAATCATTCAGAACTTTGTGCGGGTTCATGTGGATGCCCCTGGAATGGA
AGAGGGAGCCCCTGTGTTCCCTTTGGGATATCAGTACCCATCTCTGGACCAGCTTGCAGACATGATCCCTTGCGTCCTGCAGTACCTAAATTTCTCTACA
ATAATTGGAGTTGGTGTTGGAGCTGGAGCCTACATCCTGGCGAGATATGCTCTTAACCACCCGGACACTGTTGAAGGTCTTGTCCTCATCAACATTGATC
CCAATGCCAAGGGTTGGATGGATTGGGCAGCCCACAAGCTAACAGGCCTCACCTCTTCCATTCCGGAGATGATCCTTGGACATCTTTTCAGCCAGGAAGA
GCTCTCTGGAAATTCTGAGTTGATACAAAAGTACAGAAATATCATTACACATGCACCCAACCTGGATAACATTGAATTGTACTGGAACAGCTACAACAAC
CGCCGAGACCTGAACTTTGAGCGTGGAGGTGATATCACCCTCAGGTGTCCTGTGATGCTGGTGGTAGGAGACCAAGCACCTCATGAAGATGCAGTGGTGG
AATGTAACTCAAAACTGGACCCCACCCAGACCTCGTTCCTCAAGATGGCTGACTCCGGAGGTCAGCCCCAGCTGACTCAGCCAGGCAAGCTGACCGAGGC
CTTCAAGTACTTCCTGCAAGGCATGGGCTACATGGCCTCATCCTGCATGACTCGCCTGTCCCGGTCTCGTACAGCCTCTCTGACCAGTGCAGCATCCGTT
GATGGCAACCGGTCCCGCTCTCGCACCCTGTCCCAGAGCAGCGAGTCTGGAACTCTTTCTTCGGGGCCCCCGGGGCACACCATGGAGGTCTCCTGTTGAA
TGGCCCTTGTTGCCCTAGAGTGGGACCCAGCCCTCACCTCCCCCAGAGCTAACCTGGGAGGTGCTGAAGGGGCATTGGGCCACCGTAAGCAAGGGAAAAA
GGGCAGATCATGCGGGGAGATGACCTTGATCTTTGATTGCTACCCTAACCTTGACCTTTAACCCGTGATTCCCCCCAGCTCCTGGAAGAGATGTCCTAAT
ATCTCTTAGGGACCCAGACCCCTAAATTCTCCTCCTCCCCCATTTTGATGTTAAGGTGGAGAGGGCATATGCATCCTCTGTCCTGATCTAGGTGTCTATA
GCTGAGGGGTAAGAGGTTGTTGTAGTTGTCCTGGTGCCTCCATCAGACTCTCCCTACTTGTCCCATATTTGCAAGGGGAGGGGATTTGGGGCTGGGGCTC
CATTCACCAAAGCTGAGGTGGCTTCTCATTAACCCTTTAGGACTCTGAAGGGTATGGACCTACGTGAATGTGTGTCAGGGGGAGACTTGCTGGTGGGTTA
GTGGTCCTCAGGATGTGATAGAAACATCCAGTGTAAAAAGGAAGTTGGAATGGGAGTTGGCGGGCAGTGAACGAGTGTGGGGAAGGATTGGTGCTGGGGC
AACAGGAAGGGGCCTGGGGCCGTTTGGCTGCACTAACTTTGGTAGCTCAGTGTGCATCTAGAGTGGGACTGGGGAGGGAGCTAAGCTTGGGCTGGGCTGC
TTGGGGCTTGGCATAGGGTGGAAAGGGCTACCCTGGGGCTCTGACCACACTGTAGTATGTGTGGAGGGTGCCCTCCCGTCTCCCACAACTTCTGCTATAA
CAATAAACTGTAGAGGAATCTGAGTACCGTTA
  • refseq_NDRG2.F3 (forward primer)
  • refseq_NDRG2.R3 (reverse primer)
  • Single exon skipping

Frame 0

GSAGSPSRGGPESKASEGGSGRGGSRPPWRSCR
RCRSQRRSHCCQDRRLRRPRRLS*LPESSWTRDR
LTLWRHHTALSLSLSMAPPNPNAQRSLPTTMWD
STINLASSHCFSSRTCRKSFRTLCGFMWMPLEW
KREPLCSLWDISTHLWTSLQT*SLASCST*ISLQ
*LELVLELEPTSWRDMLLTTRTLLKVLSSSTLI
PMPRVGWIGQPTS*QASPLPFRR*SLDIFSARK
SSLEILS*YKSTEISLHMHPTWITLNCTGTATTT
AET*TLSVEVISPSGVL*CWW*ETKHLMKMQWW
NVTQNWTPPRPRSSRWLTPEVSPS*LSQAS*PR
PSSTSCKAWATWPHPA*LACPGLVQPL*PVQHPL
MATGPALAPCPRAASLELFLRGPRGTPWRSPVE
WPLLP*SGTQPSPPPELTWEVLKGHWATVSKGK
RADHAGR*P*SLIATLTLTFNP*FPPAPGRDVLI
SLRDPDP*ILLLPHFDVKVERAYASSVLI*VSI
AEG*EVVVVVLVPPSDSPYLSHICKGRGFGAGA
PFTKAEVASH*PFRTLKGMDLRECVSGGDLLVG*
WSSGCDRNIQCKKEVGMGVGGQ*TSVGKDWCWG
NRKGPGAVWLH*LW*LSVHLEWDWGGS*AWAGL
LGAWHRVERATLGL*PHCSMCGGCPPVSHNFCYN
NKL*RNLSTV

Frame 1

GLREARAGAGPSQRQVKVEAAAAAAGHHGGAAG
GADHRGEATVARTDA*GGQGG*VSCPNPPGPGT
DSLCGDTIRLCHFHCLWHPQTQTPSDPYLPRCGT
QL*ILLPATVSVRGHAGNHSELCAGSCGCPWNG
RGSPCVPFGISVPISGPACRHDPLRPAVPKFLY
NNWSWCWSWSLHPGEICS*PPGHC*RSCPHQH*S
QCQGLDGLGSPQANRPHLFHSGDDPWTSFQPGR
ALWKF*VDTKVQKYHYTCTQPG*H*IVLEQLQQ
PPRPEL*AWR*YHPQVSCDAGGRRPSTS*RCSGG
M*LKTGPHPDLVPQDG*LRRSAPADSARQADRG
LQVLPARHGLHGLILHDSPVPVSYSLSDQCSIR
*WQPVPLSHPVPEQRVWNSFFGAPGAHHGGLLLN
GPCCPRVGPSPHLPQS*PGRC*RGIGPP*AREK
GQIMRGDDLDL*LLP*P*PLTRDSPQLLEEMS*
YLLGTQTPKFSSSPILMLRWRGHMHPLS*SRCL*
LRGKRLL*LSWCLHQTLPTCPIFARGGDLGLGL
HSPKLRWLLINPLGL*RVWTYVNVCQGETCWWV
SGPQDVIETSSVKRKLEWELAGSERVWGRIGAGA
TGRGLGPFGCTNFGSSVCI*SGTGEGAKLGLGC
LGLGIGWKGLPWGSDHTVVCVEGALPSPTTSAI
TINCRGI*VPL

Frame 2

VCGKPEPGRARVKGK*RWKRPRRQQATMAELQE
VQITEEKPLLPGQTPEAAKEAELAARILLDQGQ
THSVETPYGSVTFTVYGTPKPKRPAILTYHDVG
LNYKSCFQPLFQFEDMQEIIQNFVRVHVDAPGME
EGAPVFPLGYQYPSLDQLADMIPCVLQYLNFST
IIGVGVGAGAYILARYALNHPDTVEGLVLINID
PNAKGWMDWAAHKLTGLTSSIPEMILGHLFSQEE
LSGNSELIQKYRNIITHAPNLDNIELYWNSYNN
RRDLNFERGGDITLRCPVMLVVGDQAPHEDAVV
ECNSKLDPTQTSFLKMADSGGQPQLTQPGKLTEA
FKYFLQGMGYMASSCMTRLSRSRTASLTSAASV
DGNRSRSRTLSQSSESGTLSSGPPGHTMEVSC*
MALVALEWDPALTSPRANLGGAEGALGHRKQGKK
GRSCGEMTLIFDCYPNLDL*PVIPPSSWKRCPN
IS*GPRPLNSPPPPF*C*GGEGICILCPDLGVY
S*GVRGCCSCPGASIRLSLLVPYLQGEGIWGWGS
IHQS*GGFSLTL*DSEGYGPT*MCVRGRLAGGL
VVLRM**KHPV*KGSWNGSWRAVNECGEGLVLG
QQEGAWGRLAALTLVAQCASRVGLGRELSLGWAA
WGLA*GGKGYPGALTTL*YVWRVPSRLPQLLL*
Q*TVEESEYR

Sequence NM_201538

GGGTCTGCGGGAAGCCCGAGCCGGGGCGGGCCCGAGTCAAAGGCAAGTGAAGGTGGAAGCGGCCGCGGCGGCAGCAGGCCACCATGGCGGAGCTGCAGGA
GGTGCAGATCACAGAGGAGAAGCCACTGTTGCCAGGACAGACGCCTGAGGCGGCCAAGACTCACTCTGTGGAGACACCATACGGCTCTGTCACTTTCACT
GTCTATGGCACCCCCAAACCCAAACGCCCAGCGATCCTTACCTACCACGATGTGGGACTCAACTATAAATCTTGCTTCCAGCCACTGTTTCAGTTCGAGG
ACATGCAGGAAATCATTCAGAACTTTGTGCGGGTTCATGTGGATGCCCCTGGAATGGAAGAGGGAGCCCCTGTGTTCCCTTTGGGATATCAGTACCCATC
TCTGGACCAGCTTGCAGACATGATCCCTTGCGTCCTGCAGTACCTAAATTTCTCTACAATAATTGGAGTTGGTGTTGGAGCTGGAGCCTACATCCTGGCG
AGATATGCTCTTAACCACCCGGACACTGTTGAAGGTCTTGTCCTCATCAACATTGATCCCAATGCCAAGGGTTGGATGGATTGGGCAGCCCACAAGCTAA
CAGGCCTCACCTCTTCCATTCCGGAGATGATCCTTGGACATCTTTTCAGCCAGGAAGAGCTCTCTGGAAATTCTGAGTTGATACAAAAGTACAGAAATAT
CATTACACATGCACCCAACCTGGATAACATTGAATTGTACTGGAACAGCTACAACAACCGCCGAGACCTGAACTTTGAGCGTGGAGGTGATATCACCCTC
AGGTGTCCTGTGATGCTGGTGGTAGGAGACCAAGCACCTCATGAAGATGCAGTGGTGGAATGTAACTCAAAACTGGACCCCACCCAGACCTCGTTCCTCA
AGATGGCTGACTCCGGAGGTCAGCCCCAGCTGACTCAGCCAGGCAAGCTGACCGAGGCCTTCAAGTACTTCCTGCAAGGCATGGGCTACATGGCCTCATC
CTGCATGACTCGCCTGTCCCGGTCTCGTACAGCCTCTCTGACCAGTGCAGCATCCGTTGATGGCAACCGGTCCCGCTCTCGCACCCTGTCCCAGAGCAGC
GAGTCTGGAACTCTTTCTTCGGGGCCCCCGGGGCACACCATGGAGGTCTCCTGTTGAATGGCCCTTGTTGCCCTAGAGTGGGACCCAGCCCTCACCTCCC
CCAGAGCTAACCTGGGAGGTGCTGAAGGGGCATTGGGCCACCGTAAGCAAGGGAAAAAGGGCAGATCATGCGGGGAGATGACCTTGATCTTTGATTGCTA
CCCTAACCTTGACCTTTAACCCGTGATTCCCCCCAGCTCCTGGAAGAGATGTCCTAATATCTCTTAGGGACCCAGACCCCTAAATTCTCCTCCTCCCCCA
TTTTGATGTTAAGGTGGAGAGGGCATATGCATCCTCTGTCCTGATCTAGGTGTCTATAGCTGAGGGGTAAGAGGTTGTTGTAGTTGTCCTGGTGCCTCCA
TCAGACTCTCCCTACTTGTCCCATATTTGCAAGGGGAGGGGATTTGGGGCTGGGGCTCCATTCACCAAAGCTGAGGTGGCTTCTCATTAACCCTTTAGGA
CTCTGAAGGGTATGGACCTACGTGAATGTGTGTCAGGGGGAGACTTGCTGGTGGGTTAGTGGTCCTCAGGATGTGATAGAAACATCCAGTGTAAAAAGGA
AGTTGGAATGGGAGTTGGCGGGCAGTGAACGAGTGTGGGGAAGGATTGGTGCTGGGGCAACAGGAAGGGGCCTGGGGCCGTTTGGCTGCACTAACTTTGG
TAGCTCAGTGTGCATCTAGAGTGGGACTGGGGAGGGAGCTAAGCTTGGGCTGGGCTGCTTGGGGCTTGGCATAGGGTGGAAAGGGCTACCCTGGGGCTCT
GACCACACTGTAGTATGTGTGGAGGGTGCCCTCCCGTCTCCCACAACTTCTGCTATAACAATAAACTGTAGAGGAATCTGAGTACCGTTA
  • refseq_NDRG2.F3 (forward primer)
  • refseq_NDRG2.R3 (reverse primer)
  • Single exon skipping

Frame 0

GSAGSPSRGGPESKASEGGSGRGGSRPPWRSCR
RCRSQRRSHCCQDRRLRRPRLTLWRHHTALSLSL
SMAPPNPNAQRSLPTTMWDSTINLASSHCFSSR
TCRKSFRTLCGFMWMPLEWKREPLCSLWDISTH
LWTSLQT*SLASCST*ISLQ*LELVLELEPTSWR
DMLLTTRTLLKVLSSSTLIPMPRVGWIGQPTS*
QASPLPFRR*SLDIFSARKSSLEILS*YKSTEI
SLHMHPTWITLNCTGTATTTAET*TLSVEVISPS
GVL*CWW*ETKHLMKMQWWNVTQNWTPPRPRSS
RWLTPEVSPS*LSQAS*PRPSSTSCKAWATWPH
PA*LACPGLVQPL*PVQHPLMATGPALAPCPRAA
SLELFLRGPRGTPWRSPVEWPLLP*SGTQPSPP
PELTWEVLKGHWATVSKGKRADHAGR*P*SLIA
TLTLTFNP*FPPAPGRDVLISLRDPDP*ILLLPH
FDVKVERAYASSVLI*VSIAEG*EVVVVVLVPP
SDSPYLSHICKGRGFGAGAPFTKAEVASH*PFR
TLKGMDLRECVSGGDLLVG*WSSGCDRNIQCKKE
VGMGVGGQ*TSVGKDWCWGNRKGPGAVWLH*LW
*LSVHLEWDWGGS*AWAGLLGAWHRVERATLGL
*PHCSMCGGCPPVSHNFCYNNKL*RNLSTV

Frame 1

GLREARAGAGPSQRQVKVEAAAAAAGHHGGAAG
GADHRGEATVARTDA*GGQDSLCGDTIRLCHFH
CLWHPQTQTPSDPYLPRCGTQL*ILLPATVSVRG
HAGNHSELCAGSCGCPWNGRGSPCVPFGISVPI
SGPACRHDPLRPAVPKFLYNNWSWCWSWSLHPG
EICS*PPGHC*RSCPHQH*SQCQGLDGLGSPQAN
RPHLFHSGDDPWTSFQPGRALWKF*VDTKVQKY
HYTCTQPG*H*IVLEQLQQPPRPEL*AWR*YHP
QVSCDAGGRRPSTS*RCSGGM*LKTGPHPDLVPQ
DG*LRRSAPADSARQADRGLQVLPARHGLHGLI
LHDSPVPVSYSLSDQCSIR*WQPVPLSHPVPEQ
RVWNSFFGAPGAHHGGLLLNGPCCPRVGPSPHLP
QS*PGRC*RGIGPP*AREKGQIMRGDDLDL*LL
P*P*PLTRDSPQLLEEMS*YLLGTQTPKFSSSP
ILMLRWRGHMHPLS*SRCL*LRGKRLL*LSWCLH
QTLPTCPIFARGGDLGLGLHSPKLRWLLINPLG
L*RVWTYVNVCQGETCWWVSGPQDVIETSSVKR
KLEWELAGSERVWGRIGAGATGRGLGPFGCTNFG
SSVCI*SGTGEGAKLGLGCLGLGIGWKGLPWGS
DHTVVCVEGALPSPTTSAITINCRGI*VPL

Frame 2

VCGKPEPGRARVKGK*RWKRPRRQQATMAELQE
VQITEEKPLLPGQTPEAAKTHSVETPYGSVTFT
VYGTPKPKRPAILTYHDVGLNYKSCFQPLFQFE
DMQEIIQNFVRVHVDAPGMEEGAPVFPLGYQYPS
LDQLADMIPCVLQYLNFSTIIGVGVGAGAYILA
RYALNHPDTVEGLVLINIDPNAKGWMDWAAHKL
TGLTSSIPEMILGHLFSQEELSGNSELIQKYRNI
ITHAPNLDNIELYWNSYNNRRDLNFERGGDITL
RCPVMLVVGDQAPHEDAVVECNSKLDPTQTSFL
KMADSGGQPQLTQPGKLTEAFKYFLQGMGYMASS
CMTRLSRSRTASLTSAASVDGNRSRSRTLSQSS
ESGTLSSGPPGHTMEVSC*MALVALEWDPALTS
PRANLGGAEGALGHRKQGKKGRSCGEMTLIFDCY
PNLDL*PVIPPSSWKRCPNIS*GPRPLNSPPPP
F*C*GGEGICILCPDLGVYS*GVRGCCSCPGAS
IRLSLLVPYLQGEGIWGWGSIHQS*GGFSLTL*D
SEGYGPT*MCVRGRLAGGLVVLRM**KHPV*KG
SWNGSWRAVNECGEGLVLGQQEGAWGRLAALTL
VAQCASRVGLGRELSLGWAAWGLA*GGKGYPGAL
TTL*YVWRVPSRLPQLLL*Q*TVEESEYR

Sequence NM_201540

GGGTCTGCGGGAAGCCCGAGCCGGGGCGGGCCCGAGTCAAAGGCAAGTGAAGGTGGAAGCGGCCGCGGCGGCAGCAGACCCCAGAGTCAGAAGGAGTGAG
AACCCTGACCCCTAATCCCACTGCATCCAGCCAATAGGAGCCCA
GCCACCATGGCGGAGCTGCAGGAGGTGCAGATCACAGAGGAGAAGCCACTGTTGCC
AGGACAGAC
GCCTGAGGCGGCCAAGGAGGCTGAGTTAGCTGCCCGAATCCTCCTGGACCAGGGACAGACTCACTCTGTGGAGACACCATACGGCTCTGTC
ACTTTCACTGTCTATGGCACCCCCAAACCCAAACGCCCAGCGATCCTTACCTACCACGATGTGGGACTCAACTATAAATCTTGCTTCCAGCCACTGTTTC
AGTTCGAGGACATGCAGGAAATCATTCAGAACTTTGTGCGGGTTCATGTGGATGCCCCTGGAATGGAAGAGGGAGCCCCTGTGTTCCCTTTGGGATATCA
GTACCCATCTCTGGACCAGCTTGCAGACATGATCCCTTGCGTCCTGCAGTACCTAAATTTCTCTACAATAATTGGAGTTGGTGTTGGAGCTGGAGCCTAC
ATCCTGGCGAGATATGCTCTTAACCACCCGGACACTGTTGAAGGTCTTGTCCTCATCAACATTGATCCCAATGCCAAGGGTTGGATGGATTGGGCAGCCC
ACAAGCTAACAGGCCTCACCTCTTCCATTCCGGAGATGATCCTTGGACATCTTTTCAGCCAGGAAGAGCTCTCTGGAAATTCTGAGTTGATACAAAAGTA
CAGAAATATCATTACACATGCACCCAACCTGGATAACATTGAATTGTACTGGAACAGCTACAACAACCGCCGAGACCTGAACTTTGAGCGTGGAGGTGAT
ATCACCCTCAGGTGTCCTGTGATGCTGGTGGTAGGAGACCAAGCACCTCATGAAGATGCAGTGGTGGAATGTAACTCAAAACTGGACCCCACCCAGACCT
CGTTCCTCAAGATGGCTGACTCCGGAGGTCAGCCCCAGCTGACTCAGCCAGGCAAGCTGACCGAGGCCTTCAAGTACTTCCTGCAAGGCATGGGCTACAT
GGCCTCATCCTGCATGACTCGCCTGTCCCGGTCTCGTACAGCCTCTCTGACCAGTGCAGCATCCGTTGATGGCAACCGGTCCCGCTCTCGCACCCTGTCC
CAGAGCAGCGAGTCTGGAACTCTTTCTTCGGGGCCCCCGGGGCACACCATGGAGGTCTCCTGTTGAATGGCCCTTGTTGCCCTAGAGTGGGACCCAGCCC
TCACCTCCCCCAGAGCTAACCTGGGAGGTGCTGAAGGGGCATTGGGCCACCGTAAGCAAGGGAAAAAGGGCAGATCATGCGGGGAGATGACCTTGATCTT
TGATTGCTACCCTAACCTTGACCTTTAACCCGTGATTCCCCCCAGCTCCTGGAAGAGATGTCCTAATATCTCTTAGGGACCCAGACCCCTAAATTCTCCT
CCTCCCCCATTTTGATGTTAAGGTGGAGAGGGCATATGCATCCTCTGTCCTGATCTAGGTGTCTATAGCTGAGGGGTAAGAGGTTGTTGTAGTTGTCCTG
GTGCCTCCATCAGACTCTCCCTACTTGTCCCATATTTGCAAGGGGAGGGGATTTGGGGCTGGGGCTCCATTCACCAAAGCTGAGGTGGCTTCTCATTAAC
CCTTTAGGACTCTGAAGGGTATGGACCTACGTGAATGTGTGTCAGGGGGAGACTTGCTGGTGGGTTAGTGGTCCTCAGGATGTGATAGAAACATCCAGTG
TAAAAAGGAAGTTGGAATGGGAGTTGGCGGGCAGTGAACGAGTGTGGGGAAGGATTGGTGCTGGGGCAACAGGAAGGGGCCTGGGGCCGTTTGGCTGCAC
TAACTTTGGTAGCTCAGTGTGCATCTAGAGTGGGACTGGGGAGGGAGCTAAGCTTGGGCTGGGCTGCTTGGGGCTTGGCATAGGGTGGAAAGGGCTACCC
TGGGGCTCTGACCACACTGTAGTATGTGTGGAGGGTGCCCTCCCGTCTCCCACAACTTCTGCTATAACAATAAACTGTAGAGGAATCTGAGTACCGTTA
  • refseq_NDRG2.F3 (forward primer)
  • refseq_NDRG2.R3 (reverse primer)
  • Single exon skipping

Frame 0

GSAGSPSRGGPESKASEGGSGRGGSRPQSQKE*
EP*PLIPLHPANRSP
ATMAELQEVQITEEKPLLP
GQT
PEAAKEAELAARILLDQGQTHSVETPYGSV
TFTVYGTPKPKRPAILTYHDVGLNYKSCFQPLF
QFEDMQEIIQNFVRVHVDAPGMEEGAPVFPLGYQ
YPSLDQLADMIPCVLQYLNFSTIIGVGVGAGAY
ILARYALNHPDTVEGLVLINIDPNAKGWMDWAA
HKLTGLTSSIPEMILGHLFSQEELSGNSELIQKY
RNIITHAPNLDNIELYWNSYNNRRDLNFERGGD
ITLRCPVMLVVGDQAPHEDAVVECNSKLDPTQT
SFLKMADSGGQPQLTQPGKLTEAFKYFLQGMGYM
ASSCMTRLSRSRTASLTSAASVDGNRSRSRTLS
QSSESGTLSSGPPGHTMEVSC*MALVALEWDPA
LTSPRANLGGAEGALGHRKQGKKGRSCGEMTLIF
DCYPNLDL*PVIPPSSWKRCPNIS*GPRPLNSP
PPPF*C*GGEGICILCPDLGVYS*GVRGCCSCP
GASIRLSLLVPYLQGEGIWGWGSIHQS*GGFSLT
L*DSEGYGPT*MCVRGRLAGGLVVLRM**KHPV
*KGSWNGSWRAVNECGEGLVLGQQEGAWGRLAA
LTLVAQCASRVGLGRELSLGWAAWGLA*GGKGYP
GALTTL*YVWRVPSRLPQLLL*Q*TVEESEYR

Frame 1

GLREARAGAGPSQRQVKVEAAAAAADPRVRRSE
NPDP*SHCIQPIGAQ
PPWRSCRRCRSQRRSHCC
QDR
RLRRPRRLS*LPESSWTRDRLTLWRHHTALS
LSLSMAPPNPNAQRSLPTTMWDSTINLASSHCF
SSRTCRKSFRTLCGFMWMPLEWKREPLCSLWDI
STHLWTSLQT*SLASCST*ISLQ*LELVLELEPT
SWRDMLLTTRTLLKVLSSSTLIPMPRVGWIGQP
TS*QASPLPFRR*SLDIFSARKSSLEILS*YKS
TEISLHMHPTWITLNCTGTATTTAET*TLSVEVI
SPSGVL*CWW*ETKHLMKMQWWNVTQNWTPPRP
RSSRWLTPEVSPS*LSQAS*PRPSSTSCKAWAT
WPHPA*LACPGLVQPL*PVQHPLMATGPALAPCP
RAASLELFLRGPRGTPWRSPVEWPLLP*SGTQP
SPPPELTWEVLKGHWATVSKGKRADHAGR*P*S
LIATLTLTFNP*FPPAPGRDVLISLRDPDP*ILL
LPHFDVKVERAYASSVLI*VSIAEG*EVVVVVL
VPPSDSPYLSHICKGRGFGAGAPFTKAEVASH*
PFRTLKGMDLRECVSGGDLLVG*WSSGCDRNIQC
KKEVGMGVGGQ*TSVGKDWCWGNRKGPGAVWLH
*LW*LSVHLEWDWGGS*AWAGLLGAWHRVERAT
LGL*PHCSMCGGCPPVSHNFCYNNKL*RNLSTV

Frame 2

VCGKPEPGRARVKGK*RWKRPRRQQTPESEGVR
TLTPNPTASSQ*EP
SHHGGAAGGADHRGEATVA
RTD
A*GGQGG*VSCPNPPGPGTDSLCGDTIRLC
HFHCLWHPQTQTPSDPYLPRCGTQL*ILLPATVS
VRGHAGNHSELCAGSCGCPWNGRGSPCVPFGIS
VPISGPACRHDPLRPAVPKFLYNNWSWCWSWSL
HPGEICS*PPGHC*RSCPHQH*SQCQGLDGLGSP
QANRPHLFHSGDDPWTSFQPGRALWKF*VDTKV
QKYHYTCTQPG*H*IVLEQLQQPPRPEL*AWR*
YHPQVSCDAGGRRPSTS*RCSGGM*LKTGPHPDL
VPQDG*LRRSAPADSARQADRGLQVLPARHGLH
GLILHDSPVPVSYSLSDQCSIR*WQPVPLSHPV
PEQRVWNSFFGAPGAHHGGLLLNGPCCPRVGPSP
HLPQS*PGRC*RGIGPP*AREKGQIMRGDDLDL
*LLP*P*PLTRDSPQLLEEMS*YLLGTQTPKFS
SSPILMLRWRGHMHPLS*SRCL*LRGKRLL*LSW
CLHQTLPTCPIFARGGDLGLGLHSPKLRWLLIN
PLGL*RVWTYVNVCQGETCWWVSGPQDVIETSS
VKRKLEWELAGSERVWGRIGAGATGRGLGPFGCT
NFGSSVCI*SGTGEGAKLGLGCLGLGIGWKGLP
WGSDHTVVCVEGALPSPTTSAITINCRGI*VPL

Sequence NM_201541

GGGTCTGCGGGAAGCCCGAGCCGGGGCGGGCCCGAGTCAAAGGCAAGTGAAGGTGGAAGCGGCCGCGGCGGCAGCAGACCCCAGAGTCAGAAGGAGTGAG
AACCCTGACCCCTAATCCCACTGCATCCAGCCAATAGGAGCCCA
GCCACCATGGCGGAGCTGCAGGAGGTGCAGATCACAGAGGAGAAGCCACTGTTGCC
AGGACAGAC
GCCTGAGGCGGCCAAGACTCACTCTGTGGAGACACCATACGGCTCTGTCACTTTCACTGTCTATGGCACCCCCAAACCCAAACGCCCAGCG
ATCCTTACCTACCACGATGTGGGACTCAACTATAAATCTTGCTTCCAGCCACTGTTTCAGTTCGAGGACATGCAGGAAATCATTCAGAACTTTGTGCGGG
TTCATGTGGATGCCCCTGGAATGGAAGAGGGAGCCCCTGTGTTCCCTTTGGGATATCAGTACCCATCTCTGGACCAGCTTGCAGACATGATCCCTTGCGT
CCTGCAGTACCTAAATTTCTCTACAATAATTGGAGTTGGTGTTGGAGCTGGAGCCTACATCCTGGCGAGATATGCTCTTAACCACCCGGACACTGTTGAA
GGTCTTGTCCTCATCAACATTGATCCCAATGCCAAGGGTTGGATGGATTGGGCAGCCCACAAGCTAACAGGCCTCACCTCTTCCATTCCGGAGATGATCC
TTGGACATCTTTTCAGCCAGGAAGAGCTCTCTGGAAATTCTGAGTTGATACAAAAGTACAGAAATATCATTACACATGCACCCAACCTGGATAACATTGA
ATTGTACTGGAACAGCTACAACAACCGCCGAGACCTGAACTTTGAGCGTGGAGGTGATATCACCCTCAGGTGTCCTGTGATGCTGGTGGTAGGAGACCAA
GCACCTCATGAAGATGCAGTGGTGGAATGTAACTCAAAACTGGACCCCACCCAGACCTCGTTCCTCAAGATGGCTGACTCCGGAGGTCAGCCCCAGCTGA
CTCAGCCAGGCAAGCTGACCGAGGCCTTCAAGTACTTCCTGCAAGGCATGGGCTACATGGCCTCATCCTGCATGACTCGCCTGTCCCGGTCTCGTACAGC
CTCTCTGACCAGTGCAGCATCCGTTGATGGCAACCGGTCCCGCTCTCGCACCCTGTCCCAGAGCAGCGAGTCTGGAACTCTTTCTTCGGGGCCCCCGGGG
CACACCATGGAGGTCTCCTGTTGAATGGCCCTTGTTGCCCTAGAGTGGGACCCAGCCCTCACCTCCCCCAGAGCTAACCTGGGAGGTGCTGAAGGGGCAT
TGGGCCACCGTAAGCAAGGGAAAAAGGGCAGATCATGCGGGGAGATGACCTTGATCTTTGATTGCTACCCTAACCTTGACCTTTAACCCGTGATTCCCCC
CAGCTCCTGGAAGAGATGTCCTAATATCTCTTAGGGACCCAGACCCCTAAATTCTCCTCCTCCCCCATTTTGATGTTAAGGTGGAGAGGGCATATGCATC
CTCTGTCCTGATCTAGGTGTCTATAGCTGAGGGGTAAGAGGTTGTTGTAGTTGTCCTGGTGCCTCCATCAGACTCTCCCTACTTGTCCCATATTTGCAAG
GGGAGGGGATTTGGGGCTGGGGCTCCATTCACCAAAGCTGAGGTGGCTTCTCATTAACCCTTTAGGACTCTGAAGGGTATGGACCTACGTGAATGTGTGT
CAGGGGGAGACTTGCTGGTGGGTTAGTGGTCCTCAGGATGTGATAGAAACATCCAGTGTAAAAAGGAAGTTGGAATGGGAGTTGGCGGGCAGTGAACGAG
TGTGGGGAAGGATTGGTGCTGGGGCAACAGGAAGGGGCCTGGGGCCGTTTGGCTGCACTAACTTTGGTAGCTCAGTGTGCATCTAGAGTGGGACTGGGGA
GGGAGCTAAGCTTGGGCTGGGCTGCTTGGGGCTTGGCATAGGGTGGAAAGGGCTACCCTGGGGCTCTGACCACACTGTAGTATGTGTGGAGGGTGCCCTC
CCGTCTCCCACAACTTCTGCTATAACAATAAACTGTAGAGGAATCTGAGTACCGTTA
  • refseq_NDRG2.F3 (forward primer)
  • refseq_NDRG2.R3 (reverse primer)
  • Single exon skipping

Frame 0

GSAGSPSRGGPESKASEGGSGRGGSRPQSQKE*
EP*PLIPLHPANRSP
ATMAELQEVQITEEKPLLP
GQT
PEAAKTHSVETPYGSVTFTVYGTPKPKRPA
ILTYHDVGLNYKSCFQPLFQFEDMQEIIQNFVR
VHVDAPGMEEGAPVFPLGYQYPSLDQLADMIPCV
LQYLNFSTIIGVGVGAGAYILARYALNHPDTVE
GLVLINIDPNAKGWMDWAAHKLTGLTSSIPEMI
LGHLFSQEELSGNSELIQKYRNIITHAPNLDNIE
LYWNSYNNRRDLNFERGGDITLRCPVMLVVGDQ
APHEDAVVECNSKLDPTQTSFLKMADSGGQPQL
TQPGKLTEAFKYFLQGMGYMASSCMTRLSRSRTA
SLTSAASVDGNRSRSRTLSQSSESGTLSSGPPG
HTMEVSC*MALVALEWDPALTSPRANLGGAEGA
LGHRKQGKKGRSCGEMTLIFDCYPNLDL*PVIPP
SSWKRCPNIS*GPRPLNSPPPPF*C*GGEGICI
LCPDLGVYS*GVRGCCSCPGASIRLSLLVPYLQ
GEGIWGWGSIHQS*GGFSLTL*DSEGYGPT*MCV
RGRLAGGLVVLRM**KHPV*KGSWNGSWRAVNE
CGEGLVLGQQEGAWGRLAALTLVAQCASRVGLG
RELSLGWAAWGLA*GGKGYPGALTTL*YVWRVPS
RLPQLLL*Q*TVEESEYR

Frame 1

GLREARAGAGPSQRQVKVEAAAAAADPRVRRSE
NPDP*SHCIQPIGAQ
PPWRSCRRCRSQRRSHCC
QDR
RLRRPRLTLWRHHTALSLSLSMAPPNPNAQR
SLPTTMWDSTINLASSHCFSSRTCRKSFRTLCG
FMWMPLEWKREPLCSLWDISTHLWTSLQT*SLA
SCST*ISLQ*LELVLELEPTSWRDMLLTTRTLLK
VLSSSTLIPMPRVGWIGQPTS*QASPLPFRR*S
LDIFSARKSSLEILS*YKSTEISLHMHPTWITL
NCTGTATTTAET*TLSVEVISPSGVL*CWW*ETK
HLMKMQWWNVTQNWTPPRPRSSRWLTPEVSPS*
LSQAS*PRPSSTSCKAWATWPHPA*LACPGLVQ
PL*PVQHPLMATGPALAPCPRAASLELFLRGPRG
TPWRSPVEWPLLP*SGTQPSPPPELTWEVLKGH
WATVSKGKRADHAGR*P*SLIATLTLTFNP*FP
PAPGRDVLISLRDPDP*ILLLPHFDVKVERAYAS
SVLI*VSIAEG*EVVVVVLVPPSDSPYLSHICK
GRGFGAGAPFTKAEVASH*PFRTLKGMDLRECV
SGGDLLVG*WSSGCDRNIQCKKEVGMGVGGQ*TS
VGKDWCWGNRKGPGAVWLH*LW*LSVHLEWDWG
GS*AWAGLLGAWHRVERATLGL*PHCSMCGGCP
PVSHNFCYNNKL*RNLSTV

Frame 2

VCGKPEPGRARVKGK*RWKRPRRQQTPESEGVR
TLTPNPTASSQ*EP
SHHGGAAGGADHRGEATVA
RTD
A*GGQDSLCGDTIRLCHFHCLWHPQTQTPS
DPYLPRCGTQL*ILLPATVSVRGHAGNHSELCAG
SCGCPWNGRGSPCVPFGISVPISGPACRHDPLR
PAVPKFLYNNWSWCWSWSLHPGEICS*PPGHC*
RSCPHQH*SQCQGLDGLGSPQANRPHLFHSGDDP
WTSFQPGRALWKF*VDTKVQKYHYTCTQPG*H*
IVLEQLQQPPRPEL*AWR*YHPQVSCDAGGRRP
STS*RCSGGM*LKTGPHPDLVPQDG*LRRSAPAD
SARQADRGLQVLPARHGLHGLILHDSPVPVSYS
LSDQCSIR*WQPVPLSHPVPEQRVWNSFFGAPG
AHHGGLLLNGPCCPRVGPSPHLPQS*PGRC*RGI
GPP*AREKGQIMRGDDLDL*LLP*P*PLTRDSP
QLLEEMS*YLLGTQTPKFSSSPILMLRWRGHMH
PLS*SRCL*LRGKRLL*LSWCLHQTLPTCPIFAR
GGDLGLGLHSPKLRWLLINPLGL*RVWTYVNVC
QGETCWWVSGPQDVIETSSVKRKLEWELAGSER
VWGRIGAGATGRGLGPFGCTNFGSSVCI*SGTGE
GAKLGLGCLGLGIGWKGLPWGSDHTVVCVEGAL
PSPTTSAITINCRGI*VPL

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