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Sequences

Sequence NM_201567

GAACAGCGAAGACAGCGTGAGCCTGGGCCGTTGCCTCGAGGCTCTCGCCCGGCTTCTCTTGCCGACCCGCCACGTTTGTTTGGATTTAATCTTCAGGTTG
CCGGCGCCCGCCCGCCCGCTGGCCTCGCGGTGTGAGAGGGAAGCACCCGTGCCTGTGGCTGGTGGCTGGCGCCTGGAGGGTCCGCACACCCGCCCGGCCG
CGCCGCTTGCCCGCGGCAGCCGCGTCCCTGAACCGCGGAGTCGTGTTTGTGTTTGACCCGCGGGCGCCGGTGGCGCGCGGCCGAGGCCGGTGTCGGCGGG
GCGGGGCGGTCGCGGCGGAGGCAGAGGAAGAGGGAGCGGGAGCTCTGCGAGGCCGGGCGCCGCCATGGAACTGGGCCCGGAGCCCCCGCACCGCCGCCGC
CTGCTCTTCGCCTGCAGCCCCCCTCCCGCGTCGCAGCCCGTCGTGAAGGCGCTATTTGGCGCTTCAGCCGCCGGGGGACTGTCGCCTGTCACCAACCTGA
CCGTCACTATGGACCAGCTGCAGGGTCTGGGCAGTGATTATGAGCAACCACTGGAGGTGAAGAACAACAGTAATCTGCAGAGAATGGGCTCCTCCGAGTC
AACAGATTCAGGTTTCTGTCTAGATTCTCCTGGGCCATTGGACAGTAAAGAAAACCTTGAAAATCCTATGAGAAGAATACATTCCCTACCTCAGAAGCTG
TTGGGATGTAGTCCAGCTCTGAAGAGGAGCCATTCTGATTCTCTTGACCATGACATCTTTCAGCTCATCGACCCAGATGAGAACAAGGAAAATCTTTCCT
CAAATGAAAGAGATAGCAGTGAACCAGGGAATTTCATTCCTCTTTTTACACCCCAGTCACCTGTGACAGCCACTTTGTCTGATGAGGATGATGGCTTCGT
GGACCTTCTCGAT
GGAGAGAATCTGAAGAATGAGGAGGAGACCCCCTCGTGCATGGCAAGCCTCTGGACAGCTCCTCTCGTCATGAGAACTACAAACCTT
GACAACCGATGCAAGCTGTTTGACTCCCCTTCCCTGTGTAGCTCCAGCACTCGGTCAGTGTTGAAGAGACCAGAACGATCTCAAGAGGAGTCTCCACCTG
GAAGTACAAAGAGGAGGAAGAGCATGTCTGGGGCCAGCCCCAAAGAGTCAACTAATCCAGAGAAGGCCCATGAGACTCTTCATCAGTCTTTATCCCTGGC
ATCTTCCCCCAAAGGAACCATTGAGAACATTTTGGACAATGACCCAAGGGACCTTATAGGAGACTTCTCCAAGGGTTATCTCTTTCATACAGTTGCTGGG
AAACATCAGGATTTAAAATACATCTCTCCAGAAATTATGGCATCTGTTTTGAATGGCAAGTTTGCCAACCTCATTAAAGAGTTTGTTATCATCGACTGTC
GATACCCATATGAATACGAGGGAGGCCACATCAAGGGTGCAGTGAACTTGCACATGGAAGAAGAGGTTGAAGACTTCTTATTGAAGAAGCCCATTGTACC
TACTGATGGCAAGCGTGTCATTGTTGTGTTTCACTGCGAGTTTTCTTCTGAGAGAGGTCCCCGCATGTGCCGGTATGTGAGAGAGAGAGATCGCCTGGGT
AATGAATACCCCAAACTCCACTACCCTGAGCTGTATGTCCTGAAGGGGGGATACAAGGAGTTCTTTATGAAATGCCAGTCTTACTGTGAGCCCCCTAGCT
ACCGGCCCATGCACCACGAGGACTTTAAAGAAGACCTGAAGAAGTTCCGCACCAAGAGCCGGACCTGGGCAGGGGAGAAGAGCAAGAGGGAGATGTACAG
TCGTCTGAAGAAGCTCTGAGGGCGGCAGGACCAGCCAGCAGCAGCCCAAGCTTCCCTCCATCCCCCTTTACCCTCTTTGCTGCAGAGAAACTTAAGCAAA
GGGGACAGCTGTGTGACATTTGGAGAGGGGGCCTGGGACTTCCATGCCTTAAACCTACCTCCCACACTCCCAAGGTTGGAGCCCAGGGCATCTTGCTGGC
TACGCCTCTTCTGTCCCTGTTAGACGTCCTCCGTCCATATCAGAACTGTGCCACAATGCAGTTCTGAGCACCGTGTCAAGCTGCTCTGAGCCACAGTGGG
ATGAACCAGCCGGGGCCTTATCGGGCTCCAGCCATCTCATGAGGGGAGAGGAGACGGAGGGGAGTAGAGAAGTTACACAGAAATGCTGCTGGCCAAATAG
CAAAGACAACCTGGGAAGGAAAGGTCTTTGTGGGATAATCCATATGTTTAATTTATTCAACTTCATCAATCACTTTATTTTATTTTTTTTTCTAACTCCT
GGAGACTTATTTTACTGCTTCATTAGGTTGAAATACTGCCATTCTAGGTAGGGTTTTATTATCCCAGGGACTACCTCGGCTTTTAATTTAAAAAAAAAAA
AGAAGTGGGTAAGAAAATGCAAACCTGTTATAAGTTATCGGACAGAAAGCTAGGTGCTCTGTCACCCCCAGGAGGCGCTGTGGTACTGGGGCTGCTGCTA
TTTAAGCCAAGAACTGAGGTCCTGGTGAGAGCGTTGGACCCAGGCTTGGCTGCCTGACATAAGCTAAATCTCCCAGACCCACCACTGGCTACCGATATCT
ATTTGGTGGGAGGTGTGGCCCTGTTCTTCCTCACCCCAGTTCCATGACATTGGCTGGTATAGGAGCCACAGTCAGGAAAGCACTTGAGGCAGCATCTGTT
GGGCCACCCCCGGCTCAGTGCTGGAATGTTGCAGTGTAGGTTTCCCAGGGAAGGGGGGTGGGGGTAGGTGGGCTCCACAGGATGGGGGAGGAGCATGTCC
ACTGAGTATCTTCCTTATGTTGCTGTGATATTGATAGCTTTTATTTTCTAATTTTTAAAAAATGGTCATATTATGAGTCAAAGAGTATCAAATCAGTGTT
GGATGGACCACCCAAGGGTGAGGAGAGGGGCTGGAAGCCCTGGGCATTAGGAGAAGGGAGTGGGTGCTGGCATGGACATGACTGGATAGAATTTTCTCAG
GAGGGAGCTTGGTGGATTTTGAAGGTAAAACTTTCTGGGTTTATCATGTTTTAATTTTAGAGACAGGGAGTGATGAATCATCACCGGTTGTCCCCTTATC
TAACTCCATAAAAGTGGGAATTTCAAAAGAACACCTCATCCAAGGAGCTGGGGCAGACTTCATTGATTCTAGAGAGACCTGTTTCAGTGCCTACTCATCC
CTGCCCTCTGGTGCCAGCCTCCTTACCATCACGGCTTCACTGAGGTGTAGGTGGGTTTTTCTTAAACAGGAGACAGTCTCTCCCCTCTTACCTCAACTTC
TTGGGGTGGGAATCAGTGATACTGGAGATGGCTAGTTGCTGTGTTACGGGTTTGAGTTACATTTGGCTATAAAACAATCTTGTTGGGAAAAATGTGGGGG
AGAGGACTTCTTCCTACACGCGCATTGAGACAGATTCCAACTGGTTAATGATATTGTTTGTAAGAAAGAGATTCTGTTGGTTGACTGCCTAAAGAGAAAG
GTGGGATGGCCTTCAGATTATACCAGCTTAGCTAGCATTACTAACCAACTGTTGGAAGCTCTGAAAATAAAAGATCTTGAACCCAT
  • refseq_CDC25A.F1 (forward primer)
  • refseq_CDC25A.R1 (reverse primer)
  • Single exon skipping

Frame 0

EQRRQREPGPLPRGSRPASLADPPRLFGFNLQV
AGARPPAGLAV*EGSTRACGWWLAPGGSAHPPGR
AACPRQPRP*TAESCLCLTRGRRWRAAEAGVGG
AGRSRRRQRKRERELCEAGRRHGTGPGAPAPPP
PALRLQPPSRVAARREGAIWRFSRRGTVACHQPD
RHYGPAAGSGQ*L*ATTGGEEQQ*SAENGLLRV
NRFRFLSRFSWAIGQ*RKP*KSYEKNTFPTSEA
VGM*SSSEEEPF*FS*P*HLSAHRPR*EQGKSFL
K*KR*Q*TREFHSSFYTPVTCDSHFV**G*WLR
GPSR
WRESEE*GGDPLVHGKPLDSSSRHENYKP
*QPMQAV*LPFPV*LQHSVSVEETRTISRGVSTW
KYKEEEEHVWGQPQRVN*SREGP*DSSSVFIPG
IFPQRNH*EHFGQ*PKGPYRRLLQGLSLSYSCW
ETSGFKIHLSRNYGICFEWQVCQPH*RVCYHRLS
IPI*IRGRPHQGCSELAHGRRG*RLLIEEAHCT
Y*WQACHCCVSLRVFF*ERSPHVPVCERERSPG
**IPQTPLP*AVCPEGGIQGVLYEMPVLL*AP*L
PAHAPRGL*RRPEEVPHQEPDLGRGEEQEGDVQ
SSEEALRAAGPASSSPSFPPSPFTLFAAEKLKQ
RGQLCDIWRGGLGLPCLKPTSHTPKVGAQGILLA
TPLLSLLDVLRPYQNCATMQF*APCQAALSHSG
MNQPGPYRAPAIS*GERRRRGVEKLHRNAAGQI
AKTTWEGKVFVG*SICLIYSTSSITLFYFFF*LL
ETYFTASLG*NTAILGRVLLSQGLPRLLI*KKK
RSG*ENANLL*VIGQKARCSVTPRRRCGTGAAA
I*AKN*GPGESVGPRLGCLT*AKSPRPTTGYRYL
FGGRCGPVLPHPSSMTLAGIGATVRKALEAASV
GPPPAQCWNVAV*VSQGRGVGVGGLHRMGEEHV
H*VSSLCCCDIDSFYFLIFKKWSYYESKSIKSVL
DGPPKGEERGWKPWALGEGSGCWHGHDWIEFSQ
EGAWWILKVKLSGFIMF*F*RQGVMNHHRLSPY
LTP*KWEFQKNTSSKELGQTSLILERPVSVPTHP
CPLVPASLPSRLH*GVGGFFLNRRQSLPSYLNF
LGWESVILEMASCCVTGLSYIWL*NNLVGKNVG
ERTSSYTRIETDSNWLMILFVRKRFCWLTA*RER
WDGLQIIPA*LALLTNCWKL*K*KILNP

Frame 1

NSEDSVSLGRCLEALARLLLPTRHVCLDLIFRL
PAPARPLASRCEREAPVPVAGGWRLEGPHTRPA
APLARGSRVPEPRSRVCV*PAGAGGARPRPVSAG
RGGRGGGRGRGSGSSARPGAAMELGPEPPHRRR
LLFACSPPPASQPVVKALFGASAAGGLSPVTNL
TVTMDQLQGLGSDYEQPLEVKNNSNLQRMGSSES
TDSGFCLDSPGPLDSKENLENPMRRIHSLPQKL
LGCSPALKRSHSDSLDHDIFQLIDPDENKENLS
SNERDSSEPGNFIPLFTPQSPVTATLSDEDDGFV
DLLD
GENLKNEEETPSCMASLWTAPLVMRTTNL
DNRCKLFDSPSLCSSSTRSVLKRPERSQEESPP
GSTKRRKSMSGASPKESTNPEKAHETLHQSLSLA
SSPKGTIENILDNDPRDLIGDFSKGYLFHTVAG
KHQDLKYISPEIMASVLNGKFANLIKEFVIIDC
RYPYEYEGGHIKGAVNLHMEEEVEDFLLKKPIVP
TDGKRVIVVFHCEFSSERGPRMCRYVRERDRLG
NEYPKLHYPELYVLKGGYKEFFMKCQSYCEPPS
YRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYS
RLKKL*GRQDQPAAAQASLHPPLPSLLQRNLSK
GDSCVTFGEGAWDFHALNLPPTLPRLEPRASCW
LRLFCPC*TSSVHIRTVPQCSSEHRVKLL*ATVG
*TSRGLIGLQPSHEGRGDGGE*RSYTEMLLAK*
QRQPGKERSLWDNPYV*FIQLHQSLYFIFFSNS
WRLILLLH*VEILPF*VGFYYPRDYLGF*FKKKK
EVGKKMQTCYKLSDRKLGALSPPGGAVVLGLLL
FKPRTEVLVRALDPGLAA*HKLNLPDPPLATDI
YLVGGVALFFLTPVP*HWLV*EPQSGKHLRQHLL
GHPRLSAGMLQCRFPREGGWG*VGSTGWGRSMS
TEYLPYVAVILIAFIF*FLKNGHIMSQRVSNQC
WMDHPRVRRGAGSPGH*EKGVGAGMDMTG*NFLR
RELGGF*R*NFLGLSCFNFRDRE**IITGCPLI
*LHKSGNFKRTPHPRSWGRLH*F*RDLFQCLLI
PALWCQPPYHHGFTEV*VGFS*TGDSLSPLTSTS
WGGNQ*YWRWLVAVLRV*VTFGYKTILLGKMWG
RGLLPTRALRQIPTG**YCL*ERDSVG*LPKEK
GGMAFRLYQLS*HY*PTVGSSENKRS*TH

Frame 2

TAKTA*AWAVASRLSPGFSCRPATFVWI*SSGC
RRPPARWPRGVRGKHPCLWLVAGAWRVRTPARP
RRLPAAAASLNRGVVFVFDPRAPVARGRGRCRR
GGAVAAEAEEEGAGALRGRAPPWNWARSPRTAAA
CSSPAAPLPRRSPS*RRYLALQPPGDCRLSPT*
PSLWTSCRVWAVIMSNHWR*RTTVICREWAPPS
QQIQVSV*ILLGHWTVKKTLKIL*EEYIPYLRSC
WDVVQL*RGAILILLTMTSFSSSTQMRTRKIFP
QMKEIAVNQGISFLFLHPSHL*QPLCLMRMMAS
WTFSM
ERI*RMRRRPPRAWQASGQLLSS*ELQTL
TTDASCLTPLPCVAPALGQC*RDQNDLKRSLHL
EVQRGGRACLGPAPKSQLIQRRPMRLFISLYPW
HLPPKEPLRTFWTMTQGTL*ETSPRVISFIQLLG
NIRI*NTSLQKLWHLF*MASLPTSLKSLLSSTV
DTHMNTREATSRVQ*TCTWKKRLKTSY*RSPLY
LLMASVSLLCFTASFLLREVPACAGM*EREIAWV
MNTPNSTTLSCMS*RGDTRSSL*NASLTVSPLA
TGPCTTRTLKKT*RSSAPRAGPGQGRRARGRCT
VV*RSSEGGRTSQQQPKLPSIPLYPLCCRET*AK
GTAV*HLERGPGTSMP*TYLPHSQGWSPGHLAG
YASSVPVRRPPSISELCHNAVLSTVSSCSEPQW
DEPAGALSGSSHLMRGEETEGSREVTQKCCWPNS
KDNLGRKGLCGIIHMFNLFNFINHFILFFFLTP
GDLFYCFIRLKYCHSR*GFIIPGTTSAFNLKKK
KKWVRKCKPVISYRTES*VLCHPQEALWYWGCCY
LSQELRSW*ERWTQAWLPDIS*ISQTHHWLPIS
IWWEVWPCSSSPQFHDIGWYRSHSQEST*GSIC
WATPGSVLECCSVGFPGKGGGGRWAPQDGGGACP
LSIFLMLL*Y**LLFSNF*KMVIL*VKEYQISV
GWTTQG*GEGLEALGIRRREWVLAWT*LDRIFS
GGSLVDFEGKTFWVYHVLILETGSDESSPVVPLS
NSIKVGISKEHLIQGAGADFIDSRETCFSAYSS
LPSGASLLTITASLRCRWVFLKQETVSPLLPQL
LGVGISDTGDG*LLCYGFELHLAIKQSCWEKCGG
EDFFLHAH*DRFQLVNDIVCKKEILLVDCLKRK
VGWPSDYTSLASITNQLLEALKIKDLEP

Sequence NM_001789

GAACAGCGAAGACAGCGTGAGCCTGGGCCGTTGCCTCGAGGCTCTCGCCCGGCTTCTCTTGCCGACCCGCCACGTTTGTTTGGATTTAATCTTCAGGTTG
CCGGCGCCCGCCCGCCCGCTGGCCTCGCGGTGTGAGAGGGAAGCACCCGTGCCTGTGGCTGGTGGCTGGCGCCTGGAGGGTCCGCACACCCGCCCGGCCG
CGCCGCTTGCCCGCGGCAGCCGCGTCCCTGAACCGCGGAGTCGTGTTTGTGTTTGACCCGCGGGCGCCGGTGGCGCGCGGCCGAGGCCGGTGTCGGCGGG
GCGGGGCGGTCGCGGCGGAGGCAGAGGAAGAGGGAGCGGGAGCTCTGCGAGGCCGGGCGCCGCCATGGAACTGGGCCCGGAGCCCCCGCACCGCCGCCGC
CTGCTCTTCGCCTGCAGCCCCCCTCCCGCGTCGCAGCCCGTCGTGAAGGCGCTATTTGGCGCTTCAGCCGCCGGGGGACTGTCGCCTGTCACCAACCTGA
CCGTCACTATGGACCAGCTGCAGGGTCTGGGCAGTGATTATGAGCAACCACTGGAGGTGAAGAACAACAGTAATCTGCAGAGAATGGGCTCCTCCGAGTC
AACAGATTCAGGTTTCTGTCTAGATTCTCCTGGGCCATTGGACAGTAAAGAAAACCTTGAAAATCCTATGAGAAGAATACATTCCCTACCTCAGAAGCTG
TTGGGATGTAGTCCAGCTCTGAAGAGGAGCCATTCTGATTCTCTTGACCATGACATCTTTCAGCTCATCGACCCAGATGAGAACAAGGAAAATGAAGCCT
TTGAGTTTAAGAAGCCAGTAAGACCTGTATCTCGTGGCTGCCTGCACTCTCATGGACTCCAGGAGGGTAAAGATCTCTTCACACAGAGGCAGAACTCTGC
CCCAGCTCGGATG
CTTTCCTCAAATGAAAGAGATAGCAGTGAACCAGGGAATTTCATTCCTCTTTTTACACCCCAGTCACCTGTGACAGCCACTTTGTCT
GATGAGGATGATGGCTTCGTGGACCTTCTCGATGGAGAGAATCTGAAGAATGAGGAGGAGACCCCCTCGTGCATGGCAAGCCTCTGGACAGCTCCTCTCG
TCATGAGAACTACAAACCTTGACAACCGATGCAAGCTGTTTGACTCCCCTTCCCTGTGTAGCTCCAGCACTCGGTCAGTGTTGAAGAGACCAGAACGATC
TCAAGAGGAGTCTCCACCTGGAAGTACAAAGAGGAGGAAGAGCATGTCTGGGGCCAGCCCCAAAGAGTCAACTAATCCAGAGAAGGCCCATGAGACTCTT
CATCAGTCTTTATCCCTGGCATCTTCCCCCAAAGGAACCATTGAGAACATTTTGGACAATGACCCAAGGGACCTTATAGGAGACTTCTCCAAGGGTTATC
TCTTTCATACAGTTGCTGGGAAACATCAGGATTTAAAATACATCTCTCCAGAAATTATGGCATCTGTTTTGAATGGCAAGTTTGCCAACCTCATTAAAGA
GTTTGTTATCATCGACTGTCGATACCCATATGAATACGAGGGAGGCCACATCAAGGGTGCAGTGAACTTGCACATGGAAGAAGAGGTTGAAGACTTCTTA
TTGAAGAAGCCCATTGTACCTACTGATGGCAAGCGTGTCATTGTTGTGTTTCACTGCGAGTTTTCTTCTGAGAGAGGTCCCCGCATGTGCCGGTATGTGA
GAGAGAGAGATCGCCTGGGTAATGAATACCCCAAACTCCACTACCCTGAGCTGTATGTCCTGAAGGGGGGATACAAGGAGTTCTTTATGAAATGCCAGTC
TTACTGTGAGCCCCCTAGCTACCGGCCCATGCACCACGAGGACTTTAAAGAAGACCTGAAGAAGTTCCGCACCAAGAGCCGGACCTGGGCAGGGGAGAAG
AGCAAGAGGGAGATGTACAGTCGTCTGAAGAAGCTCTGAGGGCGGCAGGACCAGCCAGCAGCAGCCCAAGCTTCCCTCCATCCCCCTTTACCCTCTTTGC
TGCAGAGAAACTTAAGCAAAGGGGACAGCTGTGTGACATTTGGAGAGGGGGCCTGGGACTTCCATGCCTTAAACCTACCTCCCACACTCCCAAGGTTGGA
GCCCAGGGCATCTTGCTGGCTACGCCTCTTCTGTCCCTGTTAGACGTCCTCCGTCCATATCAGAACTGTGCCACAATGCAGTTCTGAGCACCGTGTCAAG
CTGCTCTGAGCCACAGTGGGATGAACCAGCCGGGGCCTTATCGGGCTCCAGCCATCTCATGAGGGGAGAGGAGACGGAGGGGAGTAGAGAAGTTACACAG
AAATGCTGCTGGCCAAATAGCAAAGACAACCTGGGAAGGAAAGGTCTTTGTGGGATAATCCATATGTTTAATTTATTCAACTTCATCAATCACTTTATTT
TATTTTTTTTTCTAACTCCTGGAGACTTATTTTACTGCTTCATTAGGTTGAAATACTGCCATTCTAGGTAGGGTTTTATTATCCCAGGGACTACCTCGGC
TTTTAATTTAAAAAAAAAAAAGAAGTGGGTAAGAAAATGCAAACCTGTTATAAGTTATCGGACAGAAAGCTAGGTGCTCTGTCACCCCCAGGAGGCGCTG
TGGTACTGGGGCTGCTGCTATTTAAGCCAAGAACTGAGGTCCTGGTGAGAGCGTTGGACCCAGGCTTGGCTGCCTGACATAAGCTAAATCTCCCAGACCC
ACCACTGGCTACCGATATCTATTTGGTGGGAGGTGTGGCCCTGTTCTTCCTCACCCCAGTTCCATGACATTGGCTGGTATAGGAGCCACAGTCAGGAAAG
CACTTGAGGCAGCATCTGTTGGGCCACCCCCGGCTCAGTGCTGGAATGTTGCAGTGTAGGTTTCCCAGGGAAGGGGGGTGGGGGTAGGTGGGCTCCACAG
GATGGGGGAGGAGCATGTCCACTGAGTATCTTCCTTATGTTGCTGTGATATTGATAGCTTTTATTTTCTAATTTTTAAAAAATGGTCATATTATGAGTCA
AAGAGTATCAAATCAGTGTTGGATGGACCACCCAAGGGTGAGGAGAGGGGCTGGAAGCCCTGGGCATTAGGAGAAGGGAGTGGGTGCTGGCATGGACATG
ACTGGATAGAATTTTCTCAGGAGGGAGCTTGGTGGATTTTGAAGGTAAAACTTTCTGGGTTTATCATGTTTTAATTTTAGAGACAGGGAGTGATGAATCA
TCACCGGTTGTCCCCTTATCTAACTCCATAAAAGTGGGAATTTCAAAAGAACACCTCATCCAAGGAGCTGGGGCAGACTTCATTGATTCTAGAGAGACCT
GTTTCAGTGCCTACTCATCCCTGCCCTCTGGTGCCAGCCTCCTTACCATCACGGCTTCACTGAGGTGTAGGTGGGTTTTTCTTAAACAGGAGACAGTCTC
TCCCCTCTTACCTCAACTTCTTGGGGTGGGAATCAGTGATACTGGAGATGGCTAGTTGCTGTGTTACGGGTTTGAGTTACATTTGGCTATAAAACAATCT
TGTTGGGAAAAATGTGGGGGAGAGGACTTCTTCCTACACGCGCATTGAGACAGATTCCAACTGGTTAATGATATTGTTTGTAAGAAAGAGATTCTGTTGG
TTGACTGCCTAAAGAGAAAGGTGGGATGGCCTTCAGATTATACCAGCTTAGCTAGCATTACTAACCAACTGTTGGAAGCTCTGAAAATAAAAGATCTTGA
ACCCAT
  • refseq_CDC25A.F1 (forward primer)
  • refseq_CDC25A.R1 (reverse primer)
  • Single exon skipping

Frame 0

EQRRQREPGPLPRGSRPASLADPPRLFGFNLQV
AGARPPAGLAV*EGSTRACGWWLAPGGSAHPPGR
AACPRQPRP*TAESCLCLTRGRRWRAAEAGVGG
AGRSRRRQRKRERELCEAGRRHGTGPGAPAPPP
PALRLQPPSRVAARREGAIWRFSRRGTVACHQPD
RHYGPAAGSGQ*L*ATTGGEEQQ*SAENGLLRV
NRFRFLSRFSWAIGQ*RKP*KSYEKNTFPTSEA
VGM*SSSEEEPF*FS*P*HLSAHRPR*EQGK*SL
*V*EASKTCISWLPALSWTPGG*RSLHTEAELC
PSSD
AFLK*KR*Q*TREFHSSFYTPVTCDSHFV
**G*WLRGPSRWRESEE*GGDPLVHGKPLDSSSR
HENYKP*QPMQAV*LPFPV*LQHSVSVEETRTI
SRGVSTWKYKEEEEHVWGQPQRVN*SREGP*DS
SSVFIPGIFPQRNH*EHFGQ*PKGPYRRLLQGLS
LSYSCWETSGFKIHLSRNYGICFEWQVCQPH*R
VCYHRLSIPI*IRGRPHQGCSELAHGRRG*RLL
IEEAHCTY*WQACHCCVSLRVFF*ERSPHVPVCE
RERSPG**IPQTPLP*AVCPEGGIQGVLYEMPV
LL*AP*LPAHAPRGL*RRPEEVPHQEPDLGRGE
EQEGDVQSSEEALRAAGPASSSPSFPPSPFTLFA
AEKLKQRGQLCDIWRGGLGLPCLKPTSHTPKVG
AQGILLATPLLSLLDVLRPYQNCATMQF*APCQ
AALSHSGMNQPGPYRAPAIS*GERRRRGVEKLHR
NAAGQIAKTTWEGKVFVG*SICLIYSTSSITLF
YFFF*LLETYFTASLG*NTAILGRVLLSQGLPR
LLI*KKKRSG*ENANLL*VIGQKARCSVTPRRRC
GTGAAAI*AKN*GPGESVGPRLGCLT*AKSPRP
TTGYRYLFGGRCGPVLPHPSSMTLAGIGATVRK
ALEAASVGPPPAQCWNVAV*VSQGRGVGVGGLHR
MGEEHVH*VSSLCCCDIDSFYFLIFKKWSYYES
KSIKSVLDGPPKGEERGWKPWALGEGSGCWHGH
DWIEFSQEGAWWILKVKLSGFIMF*F*RQGVMNH
HRLSPYLTP*KWEFQKNTSSKELGQTSLILERP
VSVPTHPCPLVPASLPSRLH*GVGGFFLNRRQS
LPSYLNFLGWESVILEMASCCVTGLSYIWL*NNL
VGKNVGERTSSYTRIETDSNWLMILFVRKRFCW
LTA*RERWDGLQIIPA*LALLTNCWKL*K*KIL
NP

Frame 1

NSEDSVSLGRCLEALARLLLPTRHVCLDLIFRL
PAPARPLASRCEREAPVPVAGGWRLEGPHTRPA
APLARGSRVPEPRSRVCV*PAGAGGARPRPVSAG
RGGRGGGRGRGSGSSARPGAAMELGPEPPHRRR
LLFACSPPPASQPVVKALFGASAAGGLSPVTNL
TVTMDQLQGLGSDYEQPLEVKNNSNLQRMGSSES
TDSGFCLDSPGPLDSKENLENPMRRIHSLPQKL
LGCSPALKRSHSDSLDHDIFQLIDPDENKENEA
FEFKKPVRPVSRGCLHSHGLQEGKDLFTQRQNSA
PARM
LSSNERDSSEPGNFIPLFTPQSPVTATLS
DEDDGFVDLLDGENLKNEEETPSCMASLWTAPL
VMRTTNLDNRCKLFDSPSLCSSSTRSVLKRPERS
QEESPPGSTKRRKSMSGASPKESTNPEKAHETL
HQSLSLASSPKGTIENILDNDPRDLIGDFSKGY
LFHTVAGKHQDLKYISPEIMASVLNGKFANLIKE
FVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFL
LKKPIVPTDGKRVIVVFHCEFSSERGPRMCRYV
RERDRLGNEYPKLHYPELYVLKGGYKEFFMKCQS
YCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEK
SKREMYSRLKKL*GRQDQPAAAQASLHPPLPSL
LQRNLSKGDSCVTFGEGAWDFHALNLPPTLPRLE
PRASCWLRLFCPC*TSSVHIRTVPQCSSEHRVK
LL*ATVG*TSRGLIGLQPSHEGRGDGGE*RSYT
EMLLAK*QRQPGKERSLWDNPYV*FIQLHQSLYF
IFFSNSWRLILLLH*VEILPF*VGFYYPRDYLG
F*FKKKKEVGKKMQTCYKLSDRKLGALSPPGGA
VVLGLLLFKPRTEVLVRALDPGLAA*HKLNLPDP
PLATDIYLVGGVALFFLTPVP*HWLV*EPQSGK
HLRQHLLGHPRLSAGMLQCRFPREGGWG*VGST
GWGRSMSTEYLPYVAVILIAFIF*FLKNGHIMSQ
RVSNQCWMDHPRVRRGAGSPGH*EKGVGAGMDM
TG*NFLRRELGGF*R*NFLGLSCFNFRDRE**I
ITGCPLI*LHKSGNFKRTPHPRSWGRLH*F*RDL
FQCLLIPALWCQPPYHHGFTEV*VGFS*TGDSL
SPLTSTSWGGNQ*YWRWLVAVLRV*VTFGYKTI
LLGKMWGRGLLPTRALRQIPTG**YCL*ERDSVG
*LPKEKGGMAFRLYQLS*HY*PTVGSSENKRS*
TH

Frame 2

TAKTA*AWAVASRLSPGFSCRPATFVWI*SSGC
RRPPARWPRGVRGKHPCLWLVAGAWRVRTPARP
RRLPAAAASLNRGVVFVFDPRAPVARGRGRCRR
GGAVAAEAEEEGAGALRGRAPPWNWARSPRTAAA
CSSPAAPLPRRSPS*RRYLALQPPGDCRLSPT*
PSLWTSCRVWAVIMSNHWR*RTTVICREWAPPS
QQIQVSV*ILLGHWTVKKTLKIL*EEYIPYLRSC
WDVVQL*RGAILILLTMTSFSSSTQMRTRKMKP
LSLRSQ*DLYLVAACTLMDSRRVKISSHRGRTL
PQLGC
FPQMKEIAVNQGISFLFLHPSHL*QPLCL
MRMMASWTFSMERI*RMRRRPPRAWQASGQLLS
S*ELQTLTTDASCLTPLPCVAPALGQC*RDQND
LKRSLHLEVQRGGRACLGPAPKSQLIQRRPMRLF
ISLYPWHLPPKEPLRTFWTMTQGTL*ETSPRVI
SFIQLLGNIRI*NTSLQKLWHLF*MASLPTSLK
SLLSSTVDTHMNTREATSRVQ*TCTWKKRLKTSY
*RSPLYLLMASVSLLCFTASFLLREVPACAGM*
EREIAWVMNTPNSTTLSCMS*RGDTRSSL*NAS
LTVSPLATGPCTTRTLKKT*RSSAPRAGPGQGRR
ARGRCTVV*RSSEGGRTSQQQPKLPSIPLYPLC
CRET*AKGTAV*HLERGPGTSMP*TYLPHSQGW
SPGHLAGYASSVPVRRPPSISELCHNAVLSTVSS
CSEPQWDEPAGALSGSSHLMRGEETEGSREVTQ
KCCWPNSKDNLGRKGLCGIIHMFNLFNFINHFI
LFFFLTPGDLFYCFIRLKYCHSR*GFIIPGTTSA
FNLKKKKKWVRKCKPVISYRTES*VLCHPQEAL
WYWGCCYLSQELRSW*ERWTQAWLPDIS*ISQT
HHWLPISIWWEVWPCSSSPQFHDIGWYRSHSQES
T*GSICWATPGSVLECCSVGFPGKGGGGRWAPQ
DGGGACPLSIFLMLL*Y**LLFSNF*KMVIL*V
KEYQISVGWTTQG*GEGLEALGIRRREWVLAWT*
LDRIFSGGSLVDFEGKTFWVYHVLILETGSDES
SPVVPLSNSIKVGISKEHLIQGAGADFIDSRET
CFSAYSSLPSGASLLTITASLRCRWVFLKQETVS
PLLPQLLGVGISDTGDG*LLCYGFELHLAIKQS
CWEKCGGEDFFLHAH*DRFQLVNDIVCKKEILL
VDCLKRKVGWPSDYTSLASITNQLLEALKIKDLE
P

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