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Sequences

Sequence NM_014914

GCTCGGAGCGGGCTCCGCGGCTTGCAAGGCGCCTGCGACTCGGTCCCAGGTCGGCGGGCGGCGCACGGCGGGCTCGCGCGGGGGCCCCGGCGCGCCGGGC
GGCGCAGTACGCAGCGCGCGGACCCACGCCACGGCCAGGAGCCCAGAGCAGCGCGGCCACACTGCCCAGGGGTCGGCCCTCGGCCCCGGCGCTCGGAGCG
CGGCGGCTGCCTGGGCTTTAATGGCTGCTCCGCGGAGCAGCGCCTAGGGCTGGAAGGCGGCTGCGGCTCAGGAAGTCACCCGAGCAAGCCTCCTTCGGGG
CCGGCCGCACCCGCCGCGGCGCGCTCCATGGGGGCGCGCTCCCCCCGGGCGGCCCGCTGACCCGGGACGCCGGGGCCCGCTCGCTCGCCGGCCGCGCGTC
CCGGCCATGAACTGAGCCCGCGGGCCAGCCCCGCGCCTGCTCCGCCCGCGCCTTTCTTCTCGCGCCTCCTCCGCCCGCCGCCGGCGGGCCCGGCTCCCCG
GGGGCTGCGGCGCCCCGGGCTCGGCGGCCCGCGGGCCCCGGGGCGCGGGGCGGCGGCGGCGGGGGGCGCGCGGCTCCGGGCGCGGCGCCTGCACCATGAA
CTACCAGCAGCAGCTGGCCAACTCGGCTGCCATCCGGGCCGAGATCCAGCGCTTCGAGTCGGTCCACCCCAACATCTACTCCATCTACGAGCTGCTGGAG
CGCGTGGAGGAGCCGGTGCTGCAGAACCAGATCCGGGAGCACGTCATCGCCATCGAAGATGCCTTCGTGAACAGCCAGGAATGGACGCTGAGTCGATCTG
TCCCGGAGCTCAAAGTGGGAATTGTGGGTAACTTGGCCAGCGGCAAGTCTGCCCTGGTGCACCGGTACCTGACGGGCACATATGTCCAGGAGGAGTCTCC
GGAAGGTGGCAGGTTCAAGAAAGAGATTGTCGTTGATGGACAGAGCTATCTGCTGCTGATCAGAGATGAAGGGGGCCCCCCGGAGGCGCAGTTTGCCATG
TGGGTGGACGCTGTTATATTTGTCTTCAGCTTGGAGGATGAAATAAGTTTCCAGACCGTTTACCACTACTACAGTCGAATGGCCAACTATCGGAACACGA
GCGAGATTCCTCTGGTTCTGGTGGGAACCCAGGATGCCATAAGTTCTGCTAACCCGAGGGTCATCGATGACGCCAGGGCGAGGAAGCTCTCCAACGACCT
GAAACGGTGCACGTACTACGAGACGTGTGCTACATACGGGCTGAATGTGGAGAGGGTCTTCCAGGACGTTGCCCAGAAGATTGTTGCCACAAGGAAGAAG
CAGCAGCTGTCCATAGGACCCTGCAAGTCGCTACCTAATTCTCCCAGCCATTCCTCCGTCTGTTCCGCGCAGGTGTCTGCCGTGCACATCAGCCAGACAA
GTAATGGAGGTGGGAGTTTAAGCGACTATTCCTCCTCCGTTCCATCGACTCCCAGCACCAGCCAGAAGGAACTTCGGATCGATGTTCCTCCCACTGCCAA
CACGCCCACGCCCGTTCGCAAGCAGTCTAAGCGCCGGTCCAACCTGTTCACCTCTCGGAAAGGGAGCGACCCAGACAAAGAGAAGAAAGGCCTGGAGAGT
CGTGCGGACAGCATTGGGAGCGGCCGAGCCATCCCAATTAAACAGGGCATGCTGTTGAAGCGAAGTGGCAAATCGTTGAATAAAGAGTGGAAAAAGAAAT
ATGTCACCCTGTGTGACAATGGCGTGCTGACCTATCATCCCAGTTTACATGATTACATGCAGAATGTTCATGGTAAGGAGATTGACCTTCTGAGAACCAC
TGTGAAAGTCCCAGGGAAGAGGCCACCCCGAGCCACGTCAGCCTGCGCACCCATCTCCAGCCCTAAAACCAATGGCCTATCCAAGGACATGAGCAGTTTA
CACATCTCACCCAA
TTCAGACACAGGGCTGGGTGACTCCGTATGCTCCAGCCCCAGTATCTCCAGCACCACCAGCCCCAAGCTCGACCCGCCCCCCTCCC
CTCACGCCAACAGAAAGAAGCACCGAAGGAAGAAAAGCACTAGCAACTTCAAAGCCGACGGCCTGTCCGGCACTGCTGAAGAACAAGAAGAAAATTTTGA
GTTTATCATTGTGTCCCTCACTGGCCAAACATGGCACTTTGAAGCCACGACGTATGAGGAGCGGGACGCCTGGGTCCAAGCCATCGAGAGCCAGATCCTG
GCCAGCCTGCAGTCGTGCGAGAGCAGCAAGAACAAGTCCCGGCTGACGAGCCAGAGCGAGGCCATGGCCCTGCAGTCGATCCGGAACATGCGCGGGAACT
CCCACTGTGTGGACTGCGAGACCCAGAATCCCAACTGGGCCAGTTTGAACTTGGGAGCCCTCATGTGCATCGAATGCTCAGGGATCCACCGGAATCTTGG
CACCCACCTTTCCCGAGTCCGATCTCTGGACCTGGATGACTGGCCAGTCGAGCTCATCAAGGTGATGTCATCCATCGGGAACGAGCTAGCCAACAGCGTC
TGGGAAGAGAGCAGCCAGGGGCGGACGAAACCATCGGTAGACTCCACAAGGGAAGAGAAGGAACGGTGGATCCGTGCCAAGTACGAGCAGAAGCTCTTCC
TGGCCCCGCTGCCCTGCACGGAGCTGTCCCTGGGCCAGCACCTGCTGCGGGCCACCGCCGACGAGGACCTGCGGACGGCCATCCTGCTGCTGGCACACGG
CTCCCGGGACGAGGTGAACGAGACCTGCGGGGAGGGAGACGGCCGCACGGCGCTGCATCTGGCCTGCCGCAAGGGGAATGTGGTCCTGGCGCAGCTCCTG
ATCTGGTACGGAGTGGACGTCACGGCCCGAGATGCCCACGGGAACACAGCTCTGGCCTACGCCCGGCAGGCCTCCAGCCAGGAGTGCATCGACGTGCTGC
TGCAGTACGGCTGCCCCGACGAGCGCTTCGTGCTCATGGCCACCCCTAACCTGTCCAGGAGAAACAATAACCGGAACAACAGCAGTGGGAGGGTGCCCAC
CATCATCTGAGGAACAGCCGTGCCCGCCTGCTCGCCGCACCTGGGACGCGGCAGCCTCGCCGCATTCTCGCTCAGAAGTCGCAGCACGTGAGTCCCGTCG
CATCCCCTCCCTCTTCCTGGTGGCCACCTCCCTCCCGCCCACCCACTCTCACCCCAAACAAAATCACAAAACCTGGACATCCCTCAAGGGGCGAAGAGGC
GGCCGGGAGACTGCAGAAGTGGCTCCTTTTCATAAACTCCCCTAAACCACACACAGGAGAGAGCGACGGGCCTCGGCCCTTTGATGATAGCACATGGCGC
AGGACCCTTGTCCTGGTGGCACAAGGGATGGGGACGCGAGGGGGAGGGGAGGCGAGGAACAAGGAGAAGGGGCAACTTTCCTTAACTGGCAGTTGAGCAC
ATAGTACATTTCCCCTCTACCAAACGGAACACTTGGATTCCATCTCTTCTCTGAGGAGCTCGACGGCATAAATCAGAAGCAAGCACAGAGTTTGTCAGGT
TTGAAGCCCCTATGATGGTGTGTGTCAAATCAGTTGTAGCTAATCTGTCCAGGGAGAATACTGGCTTCATTACACTTGTACAGCCGAGTTCTTCCCGCAT
TACTGCTGTTTAATAGAACGTGATTAGTCATCGCCGAGAAGAAAGCATATTAGCCGAGGAGGTAGTCACGCGGCACGCGCCGGTGATTGCCACGATGTGA
TTGCAATACTCTTAGAAGCACCATATTATCCCAGACATGTTCTTTCAAGCCCTTGGAGCCCTCTCTAAATTCACTGTCATCATTTAGTATCTGTTTAATT
TTTCAGTCCAAAGAGAGGAAATCAGTCGCTGAGTATTATTTGACTCCGGTCTCCTTGGTGCAAAAACAAAATGGGAAAAATAAATAAGAATAACTCAGAA
ACTCAAAAGGAAACCACAAATTCAGCTAATAATAGCATTTCGAGTATATTTCGTAAACTAAGGAAATACACAAAAGGCTGTTTTTTCCGACTGTAAGAGA
TATTTGATGTCCTTTTGCCGAGGTGGATGTGTTAGTCTCAGGCCCTCCTGGACCACGTTGCCCAAGTCACACAGGCTTCTGTGTTATGTATTTAGATAAG
ATGTGTGAAAATATATTTGAATAAAAGAAGTTCATAAATATGCATTGATTTTTGTACAGACAAATGGCACCTCTCTTAATTTATAAATTGAACTGGATGT
GAACTAATAATGTGCAACTAGTTGAGATAAGAGGGTTACAGATCATTGTACATGGAAAATATTCCCAGCAGTAAACACTTCCATTAATGTGATCTACGGC
TTTTGAAAAGGAGCATCTCAGAATAAGATGGTGGTACAATTTGCTTATTAAAATTGAGAAAGAAAAAAAAA
  • refseq_CENTG2.F2 (forward primer)
  • refseq_CENTG2.R2 (reverse primer)
  • Single exon skipping

Frame 0

ARSGLRGLQGACDSVPGRRAAHGGLARGPRRAG
RRSTQRADPRHGQEPRAARPHCPGVGPRPRRSER
GGCLGFNGCSAEQRLGLEGGCGSGSHPSKPPSG
PAAPAAARSMGARSPRAAR*PGTPGPARSPAAR
PGHELSPRASPAPAPPAPFFSRLLRPPPAGPAPR
GLRRPGLGGPRAPGRGAAAAGGARLRARRLHHE
LPAAAGQLGCHPGRDPALRVGPPQHLLHLRAAG
ARGGAGAAEPDPGARHRHRRCLREQPGMDAESIC
PGAQSGNCG*LGQRQVCPGAPVPDGHICPGGVS
GRWQVQERDCR*WTELSAADQR*RGPPGGAVCH
VGGRCYICLQLGG*NKFPDRLPLLQSNGQLSEHE
RDSSGSGGNPGCHKFC*PEGHR*RQGEEALQRP
ETVHVLRDVCYIRAECGEGLPGRCPEDCCHKEE
AAAVHRTLQVAT*FSQPFLRLFRAGVCRAHQPDK
*WRWEFKRLFLLRSIDSQHQPEGTSDRCSSHCQ
HAHARSQAV*APVQPVHLSERERPRQREERPGE
SCGQHWERPSHPN*TGHAVEAKWQIVE*RVEKEI
CHPV*QWRADLSSQFT*LHAECSW*GD*PSENH
CESPREEATPSHVSLRTHLQP*NQWPIQGHEQF
THLTQ
FRHRAG*LRMLQPQYLQHHQPQARPAPLP
SRQQKEAPKEEKH*QLQSRRPVRHC*RTRRKF*
VYHCVPHWPNMAL*SHDV*GAGRLGPSHREPDP
GQPAVVREQQEQVPADEPERGHGPAVDPEHAREL
PLCGLRDPESQLGQFELGSPHVHRMLRDPPESW
HPPFPSPISGPG*LASRAHQGDVIHRERASQQR
LGREQPGADETIGRLHKGREGTVDPCQVRAEALP
GPAALHGAVPGPAPAAGHRRRGPADGHPAAGTR
LPGRGERDLRGGRRPHGAASGLPQGECGPGAAP
DLVRSGRHGPRCPREHSSGLRPAGLQPGVHRRAA
AVRLPRRALRAHGHP*PVQEKQ*PEQQQWEGAH
HHLRNSRARLLAAPGTRQPRRILAQKSQHVSPV
ASPPSSWWPPPSRPPTLTPNKITKPGHPSRGEEA
AGRLQKWLLFINSPKPHTGESDGPRPFDDSTWR
RTLVLVAQGMGTRGGGEARNKEKGQLSLTGS*A
HSTFPLYQTEHLDSISSLRSSTA*IRSKHRVCQV
*SPYDGVCQISCS*SVQGEYWLHYTCTAEFFPH
YCCLIERD*SSPRRKHISRGGSHAARAGDCHDV
IAILLEAPYYPRHVLSSPWSPL*IHCHHLVSV*F
FSPKRGNQSLSII*LRSPWCKNKMGKINKNNSE
TQKETTNSANNSISSIFRKLRKYTKGCFFRL*E
IFDVLLPRWMC*SQALLDHVAQVTQASVLCI*IR
CVKIYLNKRSS*ICIDFCTDKWHLS*FIN*TGC
ELIMCN*LR*EGYRSLYMENIPSSKHFH*CDLR
LLKRSISE*DGGTICLLKLRKKKK

Frame 1

LGAGSAACKAPATRSQVGGRRTAGSRGGPGAPG
GAVRSARTHATARSPEQRGHTAQGSALGPGARS
AAAAWALMAAPRSSA*GWKAAAAQEVTRASLLRG
RPHPPRRAPWGRAPPGRPADPGRRGPLARRPRV
PAMN*ARGPAPRLLRPRLSSRASSARRRRARLP
GGCGAPGSAARGPRGAGRRRRGARGSGRGACTMN
YQQQLANSAAIRAEIQRFESVHPNIYSIYELLE
RVEEPVLQNQIREHVIAIEDAFVNSQEWTLSRS
VPELKVGIVGNLASGKSALVHRYLTGTYVQEESP
EGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAM
WVDAVIFVFSLEDEISFQTVYHYYSRMANYRNT
SEIPLVLVGTQDAISSANPRVIDDARARKLSNDL
KRCTYYETCATYGLNVERVFQDVAQKIVATRKK
QQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQT
SNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTAN
TPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLES
RADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKK
YVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTT
VKVPGKRPPRATSACAPISSPKTNGLSKDMSSL
HISPN
SDTGLGDSVCSSPSISSTTSPKLDPPPS
PHANRKKHRRKKSTSNFKADGLSGTAEEQEENFE
FIIVSLTGQTWHFEATTYEERDAWVQAIESQIL
ASLQSCESSKNKSRLTSQSEAMALQSIRNMRGN
SHCVDCETQNPNWASLNLGALMCIECSGIHRNLG
THLSRVRSLDLDDWPVELIKVMSSIGNELANSV
WEESSQGRTKPSVDSTREEKERWIRAKYEQKLF
LAPLPCTELSLGQHLLRATADEDLRTAILLLAHG
SRDEVNETCGEGDGRTALHLACRKGNVVLAQLL
IWYGVDVTARDAHGNTALAYARQASSQECIDVL
LQYGCPDERFVLMATPNLSRRNNNRNNSSGRVPT
II*GTAVPACSPHLGRGSLAAFSLRSRST*VPS
HPLPLPGGHLPPAHPLSPQTKSQNLDIPQGAKR
RPGDCRSGSFS*TPLNHTQERATGLGPLMIAHGA
GPLSWWHKGWGREGEGRRGTRRRGNFP*LAVEH
IVHFPSTKRNTWIPSLL*GARRHKSEASTEFVR
FEAPMMVCVKSVVANLSRENTGFITLVQPSSSRI
TAV**NVISHRREESILAEEVVTRHAPVIATM*
LQYS*KHHIIPDMFFQALGALSKFTVII*YLFN
FSVQREEISR*VLFDSGLLGAKTKWEK*IRITQK
LKRKPQIQLIIAFRVYFVN*GNTQKAVFSDCKR
YLMSFCRGGCVSLRPSWTTLPKSHRLLCYVFR*
DV*KYI*IKEVHKYALIFVQTNGTSLNL*IELDV
N**CATS*DKRVTDHCTWKIFPAVNTSINVIYG
F*KGASQNKMVVQFAY*N*ERKK

Frame 2

SERAPRLARRLRLGPRSAGGARRARAGAPARRA
AQYAARGPTPRPGAQSSAATLPRGRPSAPALGA
RRLPGL*WLLRGAAPRAGRRLRLRKSPEQASFG
AGRTRRGALHGGALPPGGPLTRDAGARSLAGRAS
RP*TEPAGQPRACSARAFLLAPPPPAAGGPGSP
GAAAPRARRPAGPGARGGGGGGRAAPGAAPAP*
TTSSSWPTRLPSGPRSSASSRSTPTSTPSTSCWS
AWRSRCCRTRSGSTSSPSKMPS*TARNGR*VDL
SRSSKWELWVTWPAASLPWCTGT*RAHMSRRSL
RKVAGSRKRLSLMDRAICC*SEMKGAPRRRSLPC
GWTLLYLSSAWRMK*VSRPFTTTTVEWPTIGTR
ARFLWFWWEPRMP*VLLTRGSSMTPGRGSSPTT
*NGARTTRRVLHTG*MWRGSSRTLPRRLLPQGRS
SSCP*DPASRYLILPAIPPSVPRRCLPCTSARQ
VMEVGV*ATIPPPFHRLPAPARRNFGSMFLPLP
TRPRPFASSLSAGPTCSPLGKGATQTKRRKAWRV
VRTALGAAEPSQLNRACC*SEVANR*IKSGKRN
MSPCVTMAC*PIIPVYMITCRMFMVRRLTF*EP
L*KSQGRGHPEPRQPAHPSPALKPMAYPRT*AVY
TSHP
IQTQGWVTPYAPAPVSPAPPAPSSTRPPP
LTPTERSTEGRKALATSKPTACPALLKNKKKIL
SLSLCPSLAKHGTLKPRRMRSGTPGSKPSRARSW
PACSRARAARTSPG*RARARPWPCSRSGTCAGT
PTVWTARPRIPTGPV*TWEPSCASNAQGSTGIL
APTFPESDLWTWMTGQSSSSR*CHPSGTS*PTAS
GKRAARGGRNHR*TPQGKRRNGGSVPSTSRSSS
WPRCPARSCPWASTCCGPPPTRTCGRPSCCWHT
APGTR*TRPAGRETAARRCIWPAARGMWSWRSS*
SGTEWTSRPEMPTGTQLWPTPGRPPARSASTCC
CSTAAPTSASCSWPPLTCPGETITGTTAVGGCP
PSSEEQPCPPARRTWDAAASPHSRSEVAARESRR
IPSLFLVATSLPPTHSHPKQNHKTWTSLKGRRG
GRETAEVAPFHKLP*TTHRRERRASAL***HMA
QDPCPGGTRDGDARGRGGEEQGEGATFLNWQLST
*YISPLPNGTLGFHLFSEELDGINQKQAQSLSG
LKPL*WCVSNQL*LICPGRILASLHLYSRVLPA
LLLFNRT*LVIAEKKAY*PRR*SRGTRR*LPRCD
CNTLRSTILSQTCSFKPLEPSLNSLSSFSICLI
FQSKERKSVAEYYLTPVSLVQKQNGKNK*E*LR
NSKGNHKFS***HFEYIS*TKEIHKRLFFPTVRD
I*CPFAEVDVLVSGPPGPRCPSHTGFCVMYLDK
MCENIFE*KKFINMH*FLYRQMAPLLIYKLNWM
*TNNVQLVEIRGLQIIVHGKYSQQ*TLPLM*STA
FEKEHLRIRWWYNLLIKIEKEKK

Sequence NM_001037131

GCTCGGAGCGGGCTCCGCGGCTTGCAAGGCGCCTGCGACTCGGTCCCAGGTCGGCGGGCGGCGCACGGCGGGCTCGCGCGGGGGCCCCGGCGCGCCGGGC
GGCGCAGTACGCAGCGCGCGGACCCACGCCACGGCCAGGAGCCCAGAGCAGCGCGGCCACACTGCCCAGGGGTCGGCCCTCGGCCCCGGCGCTCGGAGCG
CGGCGGCTGCCTGGGCTTTAATGGCTGCTCCGCGGAGCAGCGCCTAGGGCTGGAAGGCGGCTGCGGCTCAGGAAGTCACCCGAGCAAGCCTCCTTCGGGG
CCGGCCGCACCCGCCGCGGCGCGCTCCATGGGGGCGCGCTCCCCCCGGGCGGCCCGCTGACCCGGGACGCCGGGGCCCGCTCGCTCGCCGGCCGCGCGTC
CCGGCCATGAACTGAGCCCGCGGGCCAGCCCCGCGCCTGCTCCGCCCGCGCCTTTCTTCTCGCGCCTCCTCCGCCCGCCGCCGGCGGGCCCGGCTCCCCG
GGGGCTGCGGCGCCCCGGGCTCGGCGGCCCGCGGGCCCCGGGGCGCGGGGCGGCGGCGGCGGGGGGCGCGCGGCTCCGGGCGCGGCGCCTGCACCATGAA
CTACCAGCAGCAGCTGGCCAACTCGGCTGCCATCCGGGCCGAGATCCAGCGCTTCGAGTCGGTCCACCCCAACATCTACTCCATCTACGAGCTGCTGGAG
CGCGTGGAGGAGCCGGTGCTGCAGAACCAGATCCGGGAGCACGTCATCGCCATCGAAGATGCCTTCGTGAACAGCCAGGAATGGACGCTGAGTCGATCTG
TCCCGGAGCTCAAAGTGGGAATTGTGGGTAACTTGGCCAGCGGCAAGTCTGCCCTGGTGCACCGGTACCTGACGGGCACATATGTCCAGGAGGAGTCTCC
GGAAGGTGGCAGGTTCAAGAAAGAGATTGTCGTTGATGGACAGAGCTATCTGCTGCTGATCAGAGATGAAGGGGGCCCCCCGGAGGCGCAGTTTGCCATG
TGGGTGGACGCTGTTATATTTGTCTTCAGCTTGGAGGATGAAATAAGTTTCCAGACCGTTTACCACTACTACAGTCGAATGGCCAACTATCGGAACACGA
GCGAGATTCCTCTGGTTCTGGTGGGAACCCAGGATGCCATAAGTTCTGCTAACCCGAGGGTCATCGATGACGCCAGGGCGAGGAAGCTCTCCAACGACCT
GAAACGGTGCACGTACTACGAGACGTGTGCTACATACGGGCTGAATGTGGAGAGGGTCTTCCAGGACGTTGCCCAGAAGATTGTTGCCACAAGGAAGAAG
CAGCAGCTGTCCATAGGACCCTGCAAGTCGCTACCTAATTCTCCCAGCCATTCCTCCGTCTGTTCCGCGCAGGTGTCTGCCGTGCACATCAGCCAGACAA
GTAATGGAGGTGGGAGTTTAAGCGACTATTCCTCCTCCGTTCCATCGACTCCCAGCACCAGCCAGAAGGAACTTCGGATCGATGTTCCTCCCACTGCCAA
CACGCCCACGCCCGTTCGCAAGCAGTCTAAGCGCCGGTCCAACCTGTTCACCTCTCGGAAAGGGAGCGACCCAGACAAAGAGAAGAAAGGCCTGGAGAGT
CGTGCGGACAGCATTGGGAGCGGCCGAGCCATCCCAATTAAACAGGGCATGCTGTTGAAGCGAAGTGGCAAATCGTTGAATAAAGAGTGGAAAAAGAAAT
ATGTCACCCTGTGTGACAATGGCGTGCTGACCTATCATCCCAGTTTACATGATTACATGCAGAATGTTCATGGTAAGGAGATTGACCTTCTGAGAACCAC
TGTGAAAGTCCCAGGGAAGAGGCCACCCCGAGCCACGTCAGCCTGCGCACCCATCTCCAGCCCTAAAACCAATGGCCTATCCAAGGACATGAGCAGTTTA
CACATCTCACCCAA
TTCAGGGAATGTCACTAGTGCATCTGGGTCTCAGATGGCAAGCGGCATCAGCCTGGTCTCCTTCAACAGCCGACCCGACGGCATGC
ACCAGCGCTCCTACTCAGTCTCCAGTGCCGACCAGTGGAGTGAGGCTACGGTCATTGCAAACTCGGCCATCAGCAGTG
ACACAGGGCTGGGTGACTCCGT
ATGCTCCAGCCCCAGTATCTCCAGCACCACCAGCCCCAAGCTCGACCCGCCCCCCTCCCCTCACGCCAACAGAAAGAAGCACCGAAGGAAGAAAAGCACT
AGCAACTTCAAAGCCGA
CGGCCTGTCCGGCACTGCTGAAGAACAAGAAGAAAATTTTGAGTTTATCATTGTGTCCCTCACTGGCCAAACATGGCACTTTG
AAGCCACGACGTATGAGGAGCGGGACGCCTGGGTCCAAGCCATCGAGAGCCAGATCCTGGCCAGCCTGCAGTCGTGCGAGAGCAGCAAGAACAAGTCCCG
GCTGACGAGCCAGAGCGAGGCCATGGCCCTGCAGTCGATCCGGAACATGCGCGGGAACTCCCACTGTGTGGACTGCGAGACCCAGAATCCCAACTGGGCC
AGTTTGAACTTGGGAGCCCTCATGTGCATCGAATGCTCAGGGATCCACCGGAATCTTGGCACCCACCTTTCCCGAGTCCGATCTCTGGACCTGGATGACT
GGCCAGTCGAGCTCATCAAGGTGATGTCATCCATCGGGAACGAGCTAGCCAACAGCGTCTGGGAAGAGAGCAGCCAGGGGCGGACGAAACCATCGGTAGA
CTCCACAAGGGAAGAGAAGGAACGGTGGATCCGTGCCAAGTACGAGCAGAAGCTCTTCCTGGCCCCGCTGCCCTGCACGGAGCTGTCCCTGGGCCAGCAC
CTGCTGCGGGCCACCGCCGACGAGGACCTGCGGACGGCCATCCTGCTGCTGGCACACGGCTCCCGGGACGAGGTGAACGAGACCTGCGGGGAGGGAGACG
GCCGCACGGCGCTGCATCTGGCCTGCCGCAAGGGGAATGTGGTCCTGGCGCAGCTCCTGATCTGGTACGGAGTGGACGTCACGGCCCGAGATGCCCACGG
GAACACAGCTCTGGCCTACGCCCGGCAGGCCTCCAGCCAGGAGTGCATCGACGTGCTGCTGCAGTACGGCTGCCCCGACGAGCGCTTCGTGCTCATGGCC
ACCCCTAACCTGTCCAGGAGAAACAATAACCGGAACAACAGCAGTGGGAGGGTGCCCACCATCATCTGAGGAACAGCCGTGCCCGCCTGCTCGCCGCACC
TGGGACGCGGCAGCCTCGCCGCATTCTCGCTCAGAAGTCGCAGCACGTGAGTCCCGTCGCATCCCCTCCCTCTTCCTGGTGGCCACCTCCCTCCCGCCCA
CCCACTCTCACCCCAAACAAAATCACAAAACCTGGACATCCCTCAAGGGGCGAAGAGGCGGCCGGGAGACTGCAGAAGTGGCTCCTTTTCATAAACTCCC
CTAAACCACACACAGGAGAGAGCGACGGGCCTCGGCCCTTTGATGATAGCACATGGCGCAGGACCCTTGTCCTGGTGGCACAAGGGATGGGGACGCGAGG
GGGAGGGGAGGCGAGGAACAAGGAGAAGGGGCAACTTTCCTTAACTGGCAGTTGAGCACATAGTACATTTCCCCTCTACCAAACGGAACACTTGGATTCC
ATCTCTTCTCTGAGGAGCTCGACGGCATAAATCAGAAGCAAGCACAGAGTTTGTCAGGTTTGAAGCCCCTATGATGGTGTGTGTCAAATCAGTTGTAGCT
AATCTGTCCAGGGAGAATACTGGCTTCATTACACTTGTACAGCCGAGTTCTTCCCGCATTACTGCTGTTTAATAGAACGTGATTAGTCATCGCCGAGAAG
AAAGCATATTAGCCGAGGAGGTAGTCACGCGGCACGCGCCGGTGATTGCCACGATGTGATTGCAATACTCTTAGAAGCACCATATTATCCCAGACATGTT
CTTTCAAGCCCTTGGAGCCCTCTCTAAATTCACTGTCATCATTTAGTATCTGTTTAATTTTTCAGTCCAAAGAGAGGAAATCAGTCGCTGAGTATTATTT
GACTCCGGTCTCCTTGGTGCAAAAACAAAATGGGAAAAATAAATAAGAATAACTCAGAAACTCAAAAGGAAACCACAAATTCAGCTAATAATAGCATTTC
GAGTATATTTCGTAAACTAAGGAAATACACAAAAGGCTGTTTTTTCCGACTGTAAGAGATATTTGATGTCCTTTTGCCGAGGTGGATGTGTTAGTCTCAG
GCCCTCCTGGACCACGTTGCCCAAGTCACACAGGCTTCTGTGTTATGTATTTAGATAAGATGTGTGAAAATATATTTGAATAAAAGAAGTTCATAAATAT
GCATTGATTTTTGTACAGACAAATGGCACCTCTCTTAATTTATAAATTGAACTGGATGTGAACTAATAATGTGCAACTAGTTGAGATAAGAGGGTTACAG
ATCATTGTACATGGAAAATATTCCCAGCAGTAAACACTTCCATTAATGTGATCTACGGCTTTTGAAAAGGAGCATCTCAGAATAAGATGGTGGTACAATT
TGCTTATTAAAATTGAGAAAGAAAAAAAAA
  • refseq_CENTG2.F2 (forward primer)
  • refseq_CENTG2.R2 (reverse primer)
  • Single exon skipping

Frame 0

ARSGLRGLQGACDSVPGRRAAHGGLARGPRRAG
RRSTQRADPRHGQEPRAARPHCPGVGPRPRRSER
GGCLGFNGCSAEQRLGLEGGCGSGSHPSKPPSG
PAAPAAARSMGARSPRAAR*PGTPGPARSPAAR
PGHELSPRASPAPAPPAPFFSRLLRPPPAGPAPR
GLRRPGLGGPRAPGRGAAAAGGARLRARRLHHE
LPAAAGQLGCHPGRDPALRVGPPQHLLHLRAAG
ARGGAGAAEPDPGARHRHRRCLREQPGMDAESIC
PGAQSGNCG*LGQRQVCPGAPVPDGHICPGGVS
GRWQVQERDCR*WTELSAADQR*RGPPGGAVCH
VGGRCYICLQLGG*NKFPDRLPLLQSNGQLSEHE
RDSSGSGGNPGCHKFC*PEGHR*RQGEEALQRP
ETVHVLRDVCYIRAECGEGLPGRCPEDCCHKEE
AAAVHRTLQVAT*FSQPFLRLFRAGVCRAHQPDK
*WRWEFKRLFLLRSIDSQHQPEGTSDRCSSHCQ
HAHARSQAV*APVQPVHLSERERPRQREERPGE
SCGQHWERPSHPN*TGHAVEAKWQIVE*RVEKEI
CHPV*QWRADLSSQFT*LHAECSW*GD*PSENH
CESPREEATPSHVSLRTHLQP*NQWPIQGHEQF
THLTQ
FRECH*CIWVSDGKRHQPGLLQQPTRRHA
PALLLSLQCRPVE*GYGHCKLGHQQ*
HRAG*LR
MLQPQYLQHHQPQARPAPLPSRQQKEAPKEEKH
*QLQSR
RPVRHC*RTRRKF*VYHCVPHWPNMAL*
SHDV*GAGRLGPSHREPDPGQPAVVREQQEQVP
ADEPERGHGPAVDPEHARELPLCGLRDPESQLG
QFELGSPHVHRMLRDPPESWHPPFPSPISGPG*L
ASRAHQGDVIHRERASQQRLGREQPGADETIGR
LHKGREGTVDPCQVRAEALPGPAALHGAVPGPA
PAAGHRRRGPADGHPAAGTRLPGRGERDLRGGRR
PHGAASGLPQGECGPGAAPDLVRSGRHGPRCPR
EHSSGLRPAGLQPGVHRRAAAVRLPRRALRAHG
HP*PVQEKQ*PEQQQWEGAHHHLRNSRARLLAAP
GTRQPRRILAQKSQHVSPVASPPSSWWPPPSRP
PTLTPNKITKPGHPSRGEEAAGRLQKWLLFINS
PKPHTGESDGPRPFDDSTWRRTLVLVAQGMGTRG
GGEARNKEKGQLSLTGS*AHSTFPLYQTEHLDS
ISSLRSSTA*IRSKHRVCQV*SPYDGVCQISCS
*SVQGEYWLHYTCTAEFFPHYCCLIERD*SSPRR
KHISRGGSHAARAGDCHDVIAILLEAPYYPRHV
LSSPWSPL*IHCHHLVSV*FFSPKRGNQSLSII
*LRSPWCKNKMGKINKNNSETQKETTNSANNSIS
SIFRKLRKYTKGCFFRL*EIFDVLLPRWMC*SQ
ALLDHVAQVTQASVLCI*IRCVKIYLNKRSS*I
CIDFCTDKWHLS*FIN*TGCELIMCN*LR*EGYR
SLYMENIPSSKHFH*CDLRLLKRSISE*DGGTI
CLLKLRKKKK

Frame 1

LGAGSAACKAPATRSQVGGRRTAGSRGGPGAPG
GAVRSARTHATARSPEQRGHTAQGSALGPGARS
AAAAWALMAAPRSSA*GWKAAAAQEVTRASLLRG
RPHPPRRAPWGRAPPGRPADPGRRGPLARRPRV
PAMN*ARGPAPRLLRPRLSSRASSARRRRARLP
GGCGAPGSAARGPRGAGRRRRGARGSGRGACTMN
YQQQLANSAAIRAEIQRFESVHPNIYSIYELLE
RVEEPVLQNQIREHVIAIEDAFVNSQEWTLSRS
VPELKVGIVGNLASGKSALVHRYLTGTYVQEESP
EGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAM
WVDAVIFVFSLEDEISFQTVYHYYSRMANYRNT
SEIPLVLVGTQDAISSANPRVIDDARARKLSNDL
KRCTYYETCATYGLNVERVFQDVAQKIVATRKK
QQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQT
SNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTAN
TPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLES
RADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKK
YVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTT
VKVPGKRPPRATSACAPISSPKTNGLSKDMSSL
HISPN
SGNVTSASGSQMASGISLVSFNSRPDGM
HQRSYSVSSADQWSEATVIANSAISS
DTGLGDSV
CSSPSISSTTSPKLDPPPSPHANRKKHRRKKST
SNFKAD
GLSGTAEEQEENFEFIIVSLTGQTWHF
EATTYEERDAWVQAIESQILASLQSCESSKNKSR
LTSQSEAMALQSIRNMRGNSHCVDCETQNPNWA
SLNLGALMCIECSGIHRNLGTHLSRVRSLDLDD
WPVELIKVMSSIGNELANSVWEESSQGRTKPSVD
STREEKERWIRAKYEQKLFLAPLPCTELSLGQH
LLRATADEDLRTAILLLAHGSRDEVNETCGEGD
GRTALHLACRKGNVVLAQLLIWYGVDVTARDAHG
NTALAYARQASSQECIDVLLQYGCPDERFVLMA
TPNLSRRNNNRNNSSGRVPTII*GTAVPACSPH
LGRGSLAAFSLRSRST*VPSHPLPLPGGHLPPAH
PLSPQTKSQNLDIPQGAKRRPGDCRSGSFS*TP
LNHTQERATGLGPLMIAHGAGPLSWWHKGWGRE
GEGRRGTRRRGNFP*LAVEHIVHFPSTKRNTWIP
SLL*GARRHKSEASTEFVRFEAPMMVCVKSVVA
NLSRENTGFITLVQPSSSRITAV**NVISHRRE
ESILAEEVVTRHAPVIATM*LQYS*KHHIIPDMF
FQALGALSKFTVII*YLFNFSVQREEISR*VLF
DSGLLGAKTKWEK*IRITQKLKRKPQIQLIIAF
RVYFVN*GNTQKAVFSDCKRYLMSFCRGGCVSLR
PSWTTLPKSHRLLCYVFR*DV*KYI*IKEVHKY
ALIFVQTNGTSLNL*IELDVN**CATS*DKRVT
DHCTWKIFPAVNTSINVIYGF*KGASQNKMVVQF
AY*N*ERKK

Frame 2

SERAPRLARRLRLGPRSAGGARRARAGAPARRA
AQYAARGPTPRPGAQSSAATLPRGRPSAPALGA
RRLPGL*WLLRGAAPRAGRRLRLRKSPEQASFG
AGRTRRGALHGGALPPGGPLTRDAGARSLAGRAS
RP*TEPAGQPRACSARAFLLAPPPPAAGGPGSP
GAAAPRARRPAGPGARGGGGGGRAAPGAAPAP*
TTSSSWPTRLPSGPRSSASSRSTPTSTPSTSCWS
AWRSRCCRTRSGSTSSPSKMPS*TARNGR*VDL
SRSSKWELWVTWPAASLPWCTGT*RAHMSRRSL
RKVAGSRKRLSLMDRAICC*SEMKGAPRRRSLPC
GWTLLYLSSAWRMK*VSRPFTTTTVEWPTIGTR
ARFLWFWWEPRMP*VLLTRGSSMTPGRGSSPTT
*NGARTTRRVLHTG*MWRGSSRTLPRRLLPQGRS
SSCP*DPASRYLILPAIPPSVPRRCLPCTSARQ
VMEVGV*ATIPPPFHRLPAPARRNFGSMFLPLP
TRPRPFASSLSAGPTCSPLGKGATQTKRRKAWRV
VRTALGAAEPSQLNRACC*SEVANR*IKSGKRN
MSPCVTMAC*PIIPVYMITCRMFMVRRLTF*EP
L*KSQGRGHPEPRQPAHPSPALKPMAYPRT*AVY
TSHP
IQGMSLVHLGLRWQAASAWSPSTADPTAC
TSAPTQSPVPTSGVRLRSLQTRPSAV
TQGWVTP
YAPAPVSPAPPAPSSTRPPPLTPTERSTEGRKAL
ATSKP
TACPALLKNKKKILSLSLCPSLAKHGTL
KPRRMRSGTPGSKPSRARSWPACSRARAARTSP
G*RARARPWPCSRSGTCAGTPTVWTARPRIPTGP
V*TWEPSCASNAQGSTGILAPTFPESDLWTWMT
GQSSSSR*CHPSGTS*PTASGKRAARGGRNHR*
TPQGKRRNGGSVPSTSRSSSWPRCPARSCPWAST
CCGPPPTRTCGRPSCCWHTAPGTR*TRPAGRET
AARRCIWPAARGMWSWRSS*SGTEWTSRPEMPT
GTQLWPTPGRPPARSASTCCCSTAAPTSASCSWP
PLTCPGETITGTTAVGGCPPSSEEQPCPPARRT
WDAAASPHSRSEVAARESRRIPSLFLVATSLPP
THSHPKQNHKTWTSLKGRRGGRETAEVAPFHKLP
*TTHRRERRASAL***HMAQDPCPGGTRDGDAR
GRGGEEQGEGATFLNWQLST*YISPLPNGTLGF
HLFSEELDGINQKQAQSLSGLKPL*WCVSNQL*L
ICPGRILASLHLYSRVLPALLLFNRT*LVIAEK
KAY*PRR*SRGTRR*LPRCDCNTLRSTILSQTC
SFKPLEPSLNSLSSFSICLIFQSKERKSVAEYYL
TPVSLVQKQNGKNK*E*LRNSKGNHKFS***HF
EYIS*TKEIHKRLFFPTVRDI*CPFAEVDVLVS
GPPGPRCPSHTGFCVMYLDKMCENIFE*KKFINM
H*FLYRQMAPLLIYKLNWM*TNNVQLVEIRGLQ
IIVHGKYSQQ*TLPLM*STAFEKEHLRIRWWYN
LLIKIEKEKK

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