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Sequence NM_004018
ACTTTCGGGGAGCCCGGCGGCTCTGGGAAGCTCACTCCTCCACTCGTACCCACACTCGACCGCGGAGCCCTTGCAGCCATGAGGGAACAGCTCAAAGGCCACGAGACTCAAACAACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGC
CATGAAACTCCGAAGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGAC
CAGCCCATGGATATCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGG
ATATGTGTCTGAACTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGC
ACATTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATC
CAAATTCCAAGACAGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGA
TCGAAGCGGCCCTCTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCA
TCAGGCCAAATGTAACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTT
TCTGGTCGAGTTGCAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTAC
TAAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACGCC
TGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATCTA
AATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTTTGAACCAGGACTCCCCCCTGAGCCAGCCTC
GTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAAACAGGAATCTGCAAGC
AGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATGATGCCCACCTCTCCCCAGAGTCCCCGGGAT
GCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCCTGGAAGACCACAATAAACAGCTGGAGTCAC
AGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTCCTCTCCTTCTACCTCTCTACAGAGGTCCGA
CAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTTCTCAGTCCTCCCCAGGACACAAGCACAGGG
TTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGACACAATGTAGGAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGA
TGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGAGCAGAATAAATGTTTTACAACTCCTGATTCCCGCATGGTTTTTATAATATTCATACAACAAA
GAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTATATTTTTGTGAAGGGTAGTGGTATTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTATTG
TTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTTATAAGAAAACTACATGTAAAATCTTGATAGCTAAATAACTTGCCATTT
CTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAGAAAGATTGTAAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAA
AACCCCTAAAAACAAAACAAACACACACACACACACATACACACACACACACAAAACTTTGAGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCC
ATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAAAGATAAGACTTCCTCTACCACCACACCAAATGACTACTACACACTGCTCATTTGAGAACTGT
CAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATAAGTATATAAATACTATAGTTATATAGATAAAGAGATACGAATTTCTAT
AGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGAAATTACTTCTAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGG
ATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACCACACTAAAACATTGTCTACAGCTCCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAAC
GAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAACATGTGAATGAATACACAGGACTTATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATTGA
TTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGATTTAATGATGCTTCAGTGGAAATCAATCAGAAGGTATTCTGACCTTGT
GAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGGGCTGGAGGGCTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCA
CTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACATAAAGTTCTCTGTAATTACAACTAAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGG
GTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCCCATGTGGGATGAGTTTTTAAATGCCACAAGACATAATTTAAAATAAATAAACTTTGGGAAAA
GGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGGTATCAACCCAGAAGCCCATGAACTGTGTTTCCATCCTTTGCATTTCTCTGCGAGTAGT
TCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTTACAAAAAAACCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAA
ACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCAAGAGGAAAAGCTAAGGACTGGTAGGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTT
TGATTTTTAAATCATTCATTCAATAGATACCACCGTGTGACCTATAATTTTGCAAATCTGTTACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGG
GCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAATAATTAATTAATTAACATCAAACACGGCTTCTCATGCTATTTCTACCT
CACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTC
TTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTCTAAGGTAACAAATTACCAAATTACATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTA
TGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAAAACTCAGATTTAAAACAAGGGGTTACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGTTT
CATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAGACACATTAGCTCTGGAGTGAGTCTGTCATAAT
ATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTTGGACATTATTTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTG
TAAATCATAATGTAACTGAAGCATAAACATCACATGGCATGTTTTGTCATTGTTTTCAGGTACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTT
AACACCAACACTGTAACATTTACGAATTATTTTTTTAAACTTCAGTTTTACTGCATTTTCACAACATATCAGACTTCACCAAATATATGCCTTACTATTG
TATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
TFGEPGGSGKLTPPLVPTLDRGALAAMREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTA
MKLRRLQKALCLDLLSLSAACDALDQHNLKQND
QPMDILQIINCLTTIYDRLEQEHNNLVNVPLCV
DMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKA
HLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSI
QIPRQLGEVASFGGSNIEPSVRSCFQFANNKPE
IEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKH
QAKCNICKECPIIGFRYRSLKHFNYDICQSCFF
SGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKV
LKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETP
ASSPQLSHDDTHSRIEHYASRLAEMENSNGSYL
NDSISPNESIDDEHLLIQHYCQSLNQDSPLSQP
RSPAQILISLESEERGELERILADLEEENRNLQA
EYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRD
AELIAEAKLLRQHKGRLEARMQILEDHNKQLES
QLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSD
SSQPMLLRVVGSQTSDSMGEEDLLSPPQDTSTG
LEEVMEQLNNSFPSSRGHNVGSLFHMADDLGRA
MESLVSVMTDEEGAE*MFYNS*FPHGFYNIHTTK
RIRQ*EFTRNKSIFL*RVVVLYCRFQ*FLSLLL
FC*QWQVLHVYAIVQKSYKKTTCKILIAK*LAI
SLYGTHFGLFKNL*QL*RKIVN*SVLYKKKLFIK
TPKNKTNTHTHTYTHTHKTLRQRIVLHPFGVIS
I*NSWLFLFLHIKDKTSSTTTPNDYYTLLI*EL
SAEWGRLEFSFHISICL*VYKYYSYIDKEIRISI
D*LFPFFKCSCHILIERNYF*SVIQAYLLGLEW
IFPGAGSQEETTPH*NIVYSSRCFSF*TTFH*Q
RK*SKVLDFFKGNM*MNTQDLLYQSE*SVGWLID
*LIDTFSFLLLAMPRFRFNDASVEINQKVF*PC
EHQKVFFNSQAVAGR**GWRAMDSQPIPVKE*A
TL*VKDWMIVHNT*SSL*LQLNYYALFSQSKGTG
WFGFCCFFRFIVPCGMSF*MPQDII*NK*TLGK
GVKQ*PHHICDTDRYQPRSP*TVFPSFAFLCE*
FHTGL*VSKKEGKLIQMLQKNPSWWIRQVKYINK
QTKIAQKRGEKLKRKS*GLVGKSFTLSCHFISF
*FLNHSFNRYHRVTYNFANLLPLTSSVISFWRV
G*HQV*LAFGEWVLSIINN*LINIKHGFSCYFYL
TLVLGCS**LCTPEFTASPLVHCVIFFFLYNSF
FFLHNFQKKTQSSKVTNYQIT*RFGFCLAFFSF
M*RWTFSLPKDF*NSDLKQGVTLHPTKKFK*VSF
ILKSEVNRVHK*FCFNLFVFLLDTLALE*VCHN
I*TKIESFIAAF*A*LIWTLFRVVFFITTKY*T
VNHNVTEA*TSHGMFCHCFQVLSSYLSIIIYCVL
TPTL*HLRIIFLNFSFTAFSQHIRLHQIYALLL
YYSTALLCISIKHAVML
Frame 1
LSGSPAALGSSLLHSYPHSTAEPLQP*GNSSKATRLKQLAGTIPK*QSSTSL*LT*IMSDSQLIGL
P*NSEDCRRPFAWIS*ACQLHVMPWTSTTSSKMT
SPWISCRLLIV*PLFMTAWSKSTTIWSTSLSAW
ICV*TGC*MFMIRDEQGGSVSCLLKLASFPCVK
HIWKTSTDTFSSKWQVQQDFVTSAGWASFCMILS
KFQDSWVKLHPLGAVTLSQVSGAASNLLIISQR
SKRPSS*TG*DWNPSPWCGCPSCTEWLLQKLPS
IRPNVTSAKSVQSLDSGTGV*STLIMTSAKAAFF
LVELQKAIKCTIPWWNIALRLHQEKMFETLPRY
*KTNFEPKGILRSIPEWATCQCRLS*RGTTWKR
LPRPLSFHTMILIHALNIMLAG*QKWKTAMDLI*
MIASLLMRA*MMNIC*SSITAKV*TRTPP*ASL
VVLPRS*FP*RVRKEGS*RES*QILRKKTGICK
QNMTV*SSSTNIKACPHCRPLLK*CPPLPRVPGM
LSSLLRPSYCVNTKAAWKPGCKSWKTTINSWSH
SYTG*GSCWSNPRQRPK*MAQRCPLLLPLYRGP
TAVSLCCSEWLAVKLRTPWVRKIFSVLPRTQAQG
*RR*WSNSTTPSLVQEDTM*EVFSTWQMIWAER
WSP*YQS*QMKKEQNKCFTTPDSRMVFIIFIQQ
RGLDSKSLQEINLYFCEG*WYYTVDFSSF*VCYC
FVNNGRFYTSMQLYKKVIRKLHVKS**LNNLPF
LYMERILGCLKIYNSYKERL*TKVCFIKKSCL*
KPLKTKQTHTHTHTHTHTKL*GSALFCILLA*YP
YEIHGFFFFCILKIRLPLPPHQMTTTHCSFENC
QLSGAGLSFHFIYLYVYKYINTIVI*IKRYEFL
*TDFFHFLNVHVTS**KEITSSQSSRLTCLV*NG
FFPEPEARRKLHHTKTLSTAPDVSHFKQLSTDN
ESKVKYWIFLKGTCE*IHRTYYIRVSNRLVG*L
ID*LIHSASCC*QCHDLDLMMLQWKSIRRYSDLV
NIRRYFLTPKQ*QDDDRAGGLWIPSPSL*RSRP
LFK*RIG*LFIIHKVLCNYN*IIMPSSHSQKEL
GGLVFVAFLDLLSHVG*VFKCHKT*FKINKLWEK
V*NSSPITFVILTGINPEAHELCFHPLHFSASS
STQVCK*VRKKAN*FKCYKKTLLGGLDRLNI*T
NKQKLLKKEEKSSRGKAKDW*EKALLFHAILFLF
DF*IIHSIDTTV*PIILQICYL*HQV*LAFGEW
ADIKCN*LLESGFCPLLIIN*LTSNTASHAIST
SLWFWGVPDNCAHLSSQLHHLSIALFSFSFIILS
FSFIIFKRKPKALR*QITKLHEDLVFVLHFFPL
CDAGPFLYPRIFKTQI*NKGLLYILLRSLSK*V
SF*NQR*IECINNFVLIFLFFF*TH*LWSESVII
FEQKLRALLLHFKHN*FGHYFVLCSL*PPSIKL
*IIM*LKHKHHMACFVIVFRY*VLT*VS*YIVF
*HQHCNIYELFF*TSVLLHFHNISDFTKYMPYYC
IIVLLYCVSQ*STQLCY
Frame 2
FRGARRLWEAHSSTRTHTRPRSPCSHEGTAQRPRDSNNLLGPSQNDRALPVFS*PE*CQILSL*DC
HETPKTAEGPLLGSLEPVSCM*CLGPAQPQAK*
PAHGYPADY*LFDHYL*PPGARAQQFGQRPSLRG
YVSELAAECL*YGTNREDPCPVF*NWHHFPV*S
TFGRQVQIPFQASGKFNRIL*PAQAGPPSA*FY
PNSKTVG*SCILWGQ*H*AKCPELLPIC***ARD
RSGPLPRLDETGTPVHGVAARPAQSGCCRNCQA
SGQM*HLQRVSNHWIQVQESKAL*L*HLPKLLF
FWSSCKRP*NALSHGGILHSDYIRRRCSRLCQGT
KKQISNQKVFCEASPNGLPASADCLRGGQHGNA
CLVPSAFTR*YSFTH*TLC*QASRNGKQQWILS
K**HLS**EHR**TFVNPALLPKFEPGLPPEPAS
*SCPDLDFLRE*GKRGARENPSRS*GRKQESAS
RI*PSKAAART*RPVPTAVPS*NDAHLSPESPG
C*AHC*GQATASTQRPPGSQDANPGRPQ*TAGVT
VTQAKAAAGATPGRGQSEWHNGVLSFYLSTEVR
QQSAYAAPSGWQSNFGLHG*GRSSQSSPGHKHR
VRGGDGATQQLLP*FKRTQCRKSFPHGR*FGQSD
GVLSISHDR*RRSRINVLQLLIPAWFL*YSYNK
ED*TVRVYKK*IYIFVKGSGIIL*ISVVSKSVI
VLLTMAGFTRLCNCTKKL*ENYM*NLDS*ITCHF
FIWNAFWVV*KFITVIKKDCKLKCAL*KKVVYK
NP*KQNKHTHTHIHTHTQNFEAAHCFASFWRDI
HMKFMAFSFFAY*R*DFLYHHTK*LLHTAHLRTV
S*VGQA*VFISYIYMSISI*IL*LYR*RDTNFY
RLTFSIF*MFMSHPNRKKLLLVSHPGLPAWSRM
DFSRSRKPGGNYTTLKHCLQLQMFLILNNFPLTT
KVK*SIGFF*REHVNEYTGLIISE*VIGWLVD*
LID*YIQLPAASNATI*I**CFSGNQSEGILTL
*TSEGIF*LPSSSRTMIGLEGYGFPAHPCEGVGH
SLSEGLDDCS*YIKFSVITTKLLCPLLTVKRNW
VVWFLLLF*IYCPMWDEFLNATRHNLK*INFGK
RCKTVAPSHL*Y*QVSTQKPMNCVSILCISLRVV
PHRFVSK*ERRQIDSNVTKKPFLVD*TG*IYKQ
TNKNCSKKRRKAQEEKLRTGRKKLYSFMPFYFF
LIFKSFIQ*IPPCDL*FCKSVTSDIKCN*LLESG
LTSSVISFWRVGFVHY**LIN*HQTRLLMLFLP
HFGFGVFLIIVHT*VHSFTTCPLRYFLFPL*FF
LFPS*FSKENPKL*GNKLPNYMKIWFLSCIFFLY
VTLDLFFTQGFLKLRFKTRGYFTSY*EV*VSKF
HSKIRGK*SA*IILF*SFCFSFRHISSGVSLS*
YLNKN*ELYCCILSIINLDIISCCVLYNHQVLNC
KS*CN*SINITWHVLSLFSGTEFLLEYHNILCF
NTNTVTFTNYFFKLQFYCIFTTYQTSPNICLTI
VL*YCFTVYLNKARSYV
Sequence NM_004017
ACTTTCGGGGAGCCCGGCGGCTCTGGGAAGCTCACTCCTCCACTCGTACCCACACTCGACCGCGGAGCCCTTGCAGCCATGAGGGAACAGCTCAAAGGCCACGAGACTCAAACAACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGC
CATGAAACTCCGAAGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGAC
CAGCCCATGGATATCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGG
ATATGTGTCTGAACTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGC
ACATTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATC
CAAATTCCAAGACAGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGA
TCGAAGCGGCCCTCTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCA
TCAGGCCAAATGTAACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTT
TCTGGTCGAGTTGCAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTAC
TAAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACGCC
TGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATCTA
AATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTTTGAACCAGGACTCCCCCCTGAGCCAGCCTC
GTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAAACAGGAATCTGCAAGC
AGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATGATGCCCACCTCTCCCCAGAGTCCCCGGGAT
GCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCCTGGAAGACCACAATAAACAGCTGGAGTCAC
AGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTCCTCTCCTTCTACCTCTCTACAGAGGTCCGA
CAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTTCTCAGTCCTCCCCAGGACACAAGCACAGGG
TTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCCCTGGAAAGCCAATGAGAGAGGACACAATGTAGGAAGTCT
TTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGAGCAGAATAAATGTTTTACAACTCCTGATTC
CCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTATATTTTTGTGAAGGGTAGTGGTATTATACT
GTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTTATAAGAAAACTACATG
TAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAGAAAGATTGTAAACTAA
AGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACATACACACACACACACAAAACTTTGAGGCAGC
GCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAAAGATAAGACTTCCTCTACCACCACACCAAA
TGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATAAGTATATAAATACTAT
AGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGAAATTACTTCTAGTCAG
TCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACCACACTAAAACATTGTCTACAGCTCCAGATG
TTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAACATGTGAATGAATACACAGGACTTATTATAT
CAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGATTTAATGATGCTTCAG
TGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGGGCTGGAGGGCTATGGA
TTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACATAAAGTTCTCTGTAATTACAACTAAATTAT
TATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCCCATGTGGGATGAGTTTTTAAATGCCACAAG
ACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGGTATCAACCCAGAAGCCCATGAACTGT
GTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTTACAAAAAAACCCTTCT
TGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCAAGAGGAAAAGCTAAGGACTGGTAGGAAAAA
GCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACCGTGTGACCTATAATTTTGCAAATCTGTTACCTCT
GACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAATAATTAATTAATTAAC
ATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCTTCACCACTTGTCCATT
GCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTCTAAGGTAACAAATTACCAAATTACATGAAG
ATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAAAACTCAGATTTAAAACAAGGGGTTACTTTA
CATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAG
ACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTTGGACATTATTTCGTGT
TGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGCATGTTTTGTCATTGTTTTCAGGTACTGAGT
TCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTTTTAAACTTCAGTTTTACTGCATTTTCACAACATA
TCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
TFGEPGGSGKLTPPLVPTLDRGALAAMREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTA
MKLRRLQKALCLDLLSLSAACDALDQHNLKQND
QPMDILQIINCLTTIYDRLEQEHNNLVNVPLCV
DMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKA
HLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSI
QIPRQLGEVASFGGSNIEPSVRSCFQFANNKPE
IEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKH
QAKCNICKECPIIGFRYRSLKHFNYDICQSCFF
SGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKV
LKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETP
ASSPQLSHDDTHSRIEHYASRLAEMENSNGSYL
NDSISPNESIDDEHLLIQHYCQSLNQDSPLSQP
RSPAQILISLESEERGELERILADLEEENRNLQA
EYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRD
AELIAEAKLLRQHKGRLEARMQILEDHNKQLES
QLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSD
SSQPMLLRVVGSQTSDSMGEEDLLSPPQDTSTG
LEEVMEQLNNSFPSSRGRNTPGKPMREDTM*EV
FSTWQMIWAERWSP*YQS*QMKKEQNKCFTTPDS
RMVFIIFIQQRGLDSKSLQEINLYFCEG*WYYT
VDFSSF*VCYCFVNNGRFYTSMQLYKKVIRKLH
VKS**LNNLPFLYMERILGCLKIYNSYKERL*TK
VCFIKKSCL*KPLKTKQTHTHTHTHTHTKL*GS
ALFCILLA*YPYEIHGFFFFCILKIRLPLPPHQ
MTTTHCSFENCQLSGAGLSFHFIYLYVYKYINTI
VI*IKRYEFL*TDFFHFLNVHVTS**KEITSSQ
SSRLTCLV*NGFFPEPEARRKLHHTKTLSTAPD
VSHFKQLSTDNESKVKYWIFLKGTCE*IHRTYYI
RVSNRLVG*LID*LIHSASCC*QCHDLDLMMLQ
WKSIRRYSDLVNIRRYFLTPKQ*QDDDRAGGLW
IPSPSL*RSRPLFK*RIG*LFIIHKVLCNYN*II
MPSSHSQKELGGLVFVAFLDLLSHVG*VFKCHK
T*FKINKLWEKV*NSSPITFVILTGINPEAHEL
CFHPLHFSASSSTQVCK*VRKKAN*FKCYKKTLL
GGLDRLNI*TNKQKLLKKEEKSSRGKAKDW*EK
ALLFHAILFLFDF*IIHSIDTTV*PIILQICYL
*HQV*LAFGEWADIKCN*LLESGFCPLLIIN*LT
SNTASHAISTSLWFWGVPDNCAHLSSQLHHLSI
ALFSFSFIILSFSFIIFKRKPKALR*QITKLHE
DLVFVLHFFPLCDAGPFLYPRIFKTQI*NKGLLY
ILLRSLSK*VSF*NQR*IECINNFVLIFLFFF*
TH*LWSESVIIFEQKLRALLLHFKHN*FGHYFV
LCSL*PPSIKL*IIM*LKHKHHMACFVIVFRY*V
LT*VS*YIVF*HQHCNIYELFF*TSVLLHFHNI
SDFTKYMPYYCIIVLLYCVSQ*STQLCY
Frame 1
LSGSPAALGSSLLHSYPHSTAEPLQP*GNSSKATRLKQLAGTIPK*QSSTSL*LT*IMSDSQLIGL
P*NSEDCRRPFAWIS*ACQLHVMPWTSTTSSKMT
SPWISCRLLIV*PLFMTAWSKSTTIWSTSLSAW
ICV*TGC*MFMIRDEQGGSVSCLLKLASFPCVK
HIWKTSTDTFSSKWQVQQDFVTSAGWASFCMILS
KFQDSWVKLHPLGAVTLSQVSGAASNLLIISQR
SKRPSS*TG*DWNPSPWCGCPSCTEWLLQKLPS
IRPNVTSAKSVQSLDSGTGV*STLIMTSAKAAFF
LVELQKAIKCTIPWWNIALRLHQEKMFETLPRY
*KTNFEPKGILRSIPEWATCQCRLS*RGTTWKR
LPRPLSFHTMILIHALNIMLAG*QKWKTAMDLI*
MIASLLMRA*MMNIC*SSITAKV*TRTPP*ASL
VVLPRS*FP*RVRKEGS*RES*QILRKKTGICK
QNMTV*SSSTNIKACPHCRPLLK*CPPLPRVPGM
LSSLLRPSYCVNTKAAWKPGCKSWKTTINSWSH
SYTG*GSCWSNPRQRPK*MAQRCPLLLPLYRGP
TAVSLCCSEWLAVKLRTPWVRKIFSVLPRTQAQG
*RR*WSNSTTPSLVQEEEIPLESQ*ERTQCRKS
FPHGR*FGQSDGVLSISHDR*RRSRINVLQLLI
PAWFL*YSYNKED*TVRVYKK*IYIFVKGSGIIL
*ISVVSKSVIVLLTMAGFTRLCNCTKKL*ENYM
*NLDS*ITCHFFIWNAFWVV*KFITVIKKDCKL
KCAL*KKVVYKNP*KQNKHTHTHIHTHTQNFEAA
HCFASFWRDIHMKFMAFSFFAY*R*DFLYHHTK
*LLHTAHLRTVS*VGQA*VFISYIYMSISI*IL
*LYR*RDTNFYRLTFSIF*MFMSHPNRKKLLLVS
HPGLPAWSRMDFSRSRKPGGNYTTLKHCLQLQM
FLILNNFPLTTKVK*SIGFF*REHVNEYTGLII
SE*VIGWLVD*LID*YIQLPAASNATI*I**CFS
GNQSEGILTL*TSEGIF*LPSSSRTMIGLEGYG
FPAHPCEGVGHSLSEGLDDCS*YIKFSVITTKL
LCPLLTVKRNWVVWFLLLF*IYCPMWDEFLNATR
HNLK*INFGKRCKTVAPSHL*Y*QVSTQKPMNC
VSILCISLRVVPHRFVSK*ERRQIDSNVTKKPF
LVD*TG*IYKQTNKNCSKKRRKAQEEKLRTGRKK
LYSFMPFYFFLIFKSFIQ*IPPCDL*FCKSVTS
DIKCN*LLESGLTSSVISFWRVGFVHY**LIN*
HQTRLLMLFLPHFGFGVFLIIVHT*VHSFTTCPL
RYFLFPL*FFLFPS*FSKENPKL*GNKLPNYMK
IWFLSCIFFLYVTLDLFFTQGFLKLRFKTRGYF
TSY*EV*VSKFHSKIRGK*SA*IILF*SFCFSFR
HISSGVSLS*YLNKN*ELYCCILSIINLDIISC
CVLYNHQVLNCKS*CN*SINITWHVLSLFSGTE
FLLEYHNILCFNTNTVTFTNYFFKLQFYCIFTTY
QTSPNICLTIVL*YCFTVYLNKARSYV
Frame 2
FRGARRLWEAHSSTRTHTRPRSPCSHEGTAQRPRDSNNLLGPSQNDRALPVFS*PE*CQILSL*DC
HETPKTAEGPLLGSLEPVSCM*CLGPAQPQAK*
PAHGYPADY*LFDHYL*PPGARAQQFGQRPSLRG
YVSELAAECL*YGTNREDPCPVF*NWHHFPV*S
TFGRQVQIPFQASGKFNRIL*PAQAGPPSA*FY
PNSKTVG*SCILWGQ*H*AKCPELLPIC***ARD
RSGPLPRLDETGTPVHGVAARPAQSGCCRNCQA
SGQM*HLQRVSNHWIQVQESKAL*L*HLPKLLF
FWSSCKRP*NALSHGGILHSDYIRRRCSRLCQGT
KKQISNQKVFCEASPNGLPASADCLRGGQHGNA
CLVPSAFTR*YSFTH*TLC*QASRNGKQQWILS
K**HLS**EHR**TFVNPALLPKFEPGLPPEPAS
*SCPDLDFLRE*GKRGARENPSRS*GRKQESAS
RI*PSKAAART*RPVPTAVPS*NDAHLSPESPG
C*AHC*GQATASTQRPPGSQDANPGRPQ*TAGVT
VTQAKAAAGATPGRGQSEWHNGVLSFYLSTEVR
QQSAYAAPSGWQSNFGLHG*GRSSQSSPGHKHR
VRGGDGATQQLLP*FKRKKYPWKANERGHNVGSL
FHMADDLGRAMESLVSVMTDEEGAE*MFYNS*F
PHGFYNIHTTKRIRQ*EFTRNKSIFL*RVVVLY
CRFQ*FLSLLLFC*QWQVLHVYAIVQKSYKKTTC
KILIAK*LAISLYGTHFGLFKNL*QL*RKIVN*
SVLYKKKLFIKTPKNKTNTHTHTYTHTHKTLRQ
RIVLHPFGVISI*NSWLFLFLHIKDKTSSTTTPN
DYYTLLI*ELSAEWGRLEFSFHISICL*VYKYY
SYIDKEIRISID*LFPFFKCSCHILIERNYF*S
VIQAYLLGLEWIFPGAGSQEETTPH*NIVYSSRC
FSF*TTFH*QRK*SKVLDFFKGNM*MNTQDLLY
QSE*SVGWLID*LIDTFSFLLLAMPRFRFNDAS
VEINQKVF*PCEHQKVFFNSQAVAGR**GWRAMD
SQPIPVKE*ATL*VKDWMIVHNT*SSL*LQLNY
YALFSQSKGTGWFGFCCFFRFIVPCGMSF*MPQ
DII*NK*TLGKGVKQ*PHHICDTDRYQPRSP*TV
FPSFAFLCE*FHTGL*VSKKEGKLIQMLQKNPS
WWIRQVKYINKQTKIAQKRGEKLKRKS*GLVGK
SFTLSCHFISF*FLNHSFNRYHRVTYNFANLLPL
TSSVISFWRVG*HQV*LAFGEWVLSIINN*LIN
IKHGFSCYFYLTLVLGCS**LCTPEFTASPLVH
CVIFFFLYNSFFFLHNFQKKTQSSKVTNYQIT*R
FGFCLAFFSFM*RWTFSLPKDF*NSDLKQGVTL
HPTKKFK*VSFILKSEVNRVHK*FCFNLFVFLL
DTLALE*VCHNI*TKIESFIAAF*A*LIWTLFRV
VFFITTKY*TVNHNVTEA*TSHGMFCHCFQVLS
SYLSIIIYCVLTPTL*HLRIIFLNFSFTAFSQH
IRLHQIYALLLYYSTALLCISIKHAVML
Sequence NM_004022
ACACTAGCAATGGCAAAGCTTTGTGCGGAGGCATTGCTGGCTGCTCTGAACTAAAAGCATCCGTGGGGACCGAAAGAGGTTTTTGCACACCTTATTAAGGAACTCCAGGATGGCATTGGGCAGCGGCAAACTGTTGTCAGAACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGATGCCAGTAT
TCTACAGGAAAAATTGGGAAGCCTGAATCTGCGGTGGCAGGAGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGGCTAGAAGAACAAAAGAATATCTTG
TCAGAATTTCAAAGAGATTTAAATGAATTTGTTTTATGGTTGGAGGAAGCAGATAACATTGCTAGTATCCCACTTGAACCTGGAAAAGAGCAGCAACTAA
AAGAAAAGCTTGAGCAAGTCAAGTTACTGGTGGAAGAGTTGCCCCTGCGCCAGGGAATTCTCAAACAATTAAATGAAACTGGAGGACCCGTGCTTGTAAG
TGCTCCCATAAGCCCAGAAGAGCAAGATAAACTTGAAAATAAGCTCAAGCAGACAAATCTCCAGTGGATAAAGGTTTCCAGAGCTTTACCTGAGAAACAA
GGAGAAATTGAAGCTCAAATAAAAGACCTTGGGCAGCTTGAAAAAAAGCTTGAAGACCTTGAAGAGCAGTTAAATCATCTGCTGCTGTGGTTATCTCCTA
TTAGGAATCAGTTGGAAATTTATAACCAACCAAACCAAGAAGGACCATTTGACGTTAAGGAAACTGAAATAGCAGTTCAAGCTAAACAACCGGATGTGGA
AGAGATTTTGTCTAAAGGGCAGCATTTGTACAAGGAAAAACCAGCCACTCAGCCAGTGAAGAGGAAGTTAGAAGATCTGAGCTCTGAGTGGAAGGCGGTA
AACCGTTTACTTCAAGAGCTGAGGGCAAAGCAGCCTGACCTAGCTCCTGGACTGACCACTATTGGAGCCTCTCCTACTCAGACTGTTACTCTGGTGACAC
AACCTGTGGTTACTAAGGAAACTGCCATCTCCAAACTAGAAATGCCATCTTCCTTGATGTTGGAGGTACCTGCTCTGGCAGATTTCAACCGGGCTTGGAC
AGAACTTACCGACTGGCTTTCTCTGCTTGATCAAGTTATAAAATCACAGAGGGTGATGGTGGGTGACCTTGAGGATATCAACGAGATGATCATCAAGCAG
AAGGCAACAATGCAGGATTTGGAACAGAGGCGTCCCCAGTTGGAAGAACTCATTACCGCTGCCCAAAATTTGAAAAACAAGACCAGCAATCAAGAGGCTA
GAACAATCATTACGGATCGAATTGAAAGAATTCAGAATCAGTGGGATGAAGTACAAGAACACCTTCAGAACCGGAGGCAACAGTTGAATGAAATGTTAAA
GGATTCAACACAATGGCTGGAAGCTAAGGAAGAAGCTGAGCAGGTCTTAGGACAGGCCAGAGCCAAGCTTGAGTCATGGAAGGAGGGTCCCTATACAGTA
GATGCAATCCAAAAGAAAATCACAGAAACCAAGCAGTTGGCCAAAGACCTCCGCCAGTGGCAGACAAATGTAGATGTGGCAAATGACTTGGCCCTGAAAC
TTCTCCGGGATTATTCTGCAGATGATACCAGAAAAGTCCACATGATAACAGAGAATATCAATGCCTCTTGGAGAAGCATTCATAAAAGGGTGAGTGAGCG
AGAGGCTGCTTTGGAAGAAACTCATAGATTACTGCAACAGTTCCCCCTGGACCTGGAAAAGTTTCTTGCCTGGCTTACAGAAGCTGAAACAACTGCCAAT
GTCCTACAGGATGCTACCCGTAAGGAAAGGCTCCTAGAAGACTCCAAGGGAGTAAAAGAGCTGATGAAACAATGGCAAGACCTCCAAGGTGAAATTGAAG
CTCACACAGATGTTTATCACAACCTGGATGAAAACAGCCAAAAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTACAAAGACGTTTGGA
TAACATGAACTTCAAGTGGAGTGAACTTCGGAAAAAGTCTCTCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCGTCTGCACCTTTCT
CTGCAGGAACTTCTGGTGTGGCTACAGCTGAAAGATGATGAATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAGAAGCAGAACGATG
TACATAGGGCCTTCAAGAGGGAATTGAAAACTAAAGAACCTGTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGCAGCCTTTGGAAGG
ACTAGAGAAACTCTACCAGGAGCCCAGAGAGCTGCCTCCTGAGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGAGGAGGTCAATACT
GAGTGGGAAAAATTGAACCTGCACTCCGCTGACTGGCAGAGAAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCCACGGATGAGCTGG
ACCTCAAGCTGCGCCAAGCTGAGGTGATCAAGGGATCCTGGCAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCGAGAAAGTCAAGGC
ACTTCGAGGAGAAATTGCGCCTCTGAAAGAGAACGTGAGCCACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCTCTCACCGTATAAC
CTCAGCACTCTGGAAGACCTGAACACCAGATGGAAGCTTCTGCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCACAGGGACTTTGGTC
CAGCATCTCAGCACTTTCTTTCCACGTCTGTCCAGGGTCCCTGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACCACGAGACTCAAAC
AACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGCCATGAAACTCCGA
AGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGACCAGCCCATGGATA
TCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGGATATGTGTCTGAA
CTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGCACATTTGGAAGAC
AAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATCCAAATTCCAAGAC
AGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGATCGAAGCGGCCCT
CTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCATCAGGCCAAATGT
AACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGTCGAGTTG
CAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTAAAAAACAAATT
TCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACGCCTGCCTCGTCCCCT
CAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATCTAAATGATAGCATCT
CTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTTTGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCA
GATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAAACAGGAATCTGCAAGCAGAATATGACCGT
CTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATGATGCCCACCTCTCCCCAGAGTCCCCGGGATGCTGAGCTCATTG
CTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCCTGGAAGACCACAATAAACAGCTGGAGTCACAGTTACACAGGCT
AAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTCCTCTCCTTCTACCTCTCTACAGAGGTCCGACAGCAGTCAGCCT
ATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTTCTCAGTCCTCCCCAGGACACAAGCACAGGGTTAGAGGAGGTGA
TGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGACACAATGTAGGAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGT
ATCAGTCATGACAGATGAAGAAGGAGCAGAATAAATGTTTTACAACTCCTGATTCCCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAG
TAAGAGTTTACAAGAAATAAATCTATATTTTTGTGAAGGGTAGTGGTATTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAAT
GGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTTATAAGAAAACTACATGTAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAAC
GCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAGAAAGATTGTAAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAAC
AAAACAAACACACACACACACACATACACACACACACACAAAACTTTGAGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCAT
GGCTTTTTCTTTTTTTGCATATTAAAGATAAGACTTCCTCTACCACCACACCAAATGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGG
CAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATAAGTATATAAATACTATAGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTT
CCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGAAATTACTTCTAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAG
CCGGAAGCCAGGAGGAAACTACACCACACTAAAACATTGTCTACAGCTCCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAA
AGTATTGGATTTTTTTAAAGGGAACATGTGAATGAATACACAGGACTTATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGAT
ACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGATTTAATGATGCTTCAGTGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGG
TATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGGGCTGGAGGGCTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAA
GGATTGGATGATTGTTCATAATACATAAAGTTCTCTGTAATTACAACTAAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTT
TGTTGCTTTTTTAGATTTATTGTCCCATGTGGGATGAGTTTTTAAATGCCACAAGACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGT
AGCCCCATCACATTTGTGATACTGACAGGTATCAACCCAGAAGCCCATGAACTGTGTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTT
GTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTTACAAAAAAACCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATT
GCTCAAAAAAGAGGAGAAAAGCTCAAGAGGAAAAGCTAAGGACTGGTAGGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATC
ATTCATTCAATAGATACCACCGTGTGACCTATAATTTTGCAAATCTGTTACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGT
GTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAATAATTAATTAATTAACATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTG
GGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATA
ATTTTCAAAAGAAAACCCAAAGCTCTAAGGTAACAAATTACCAAATTACATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGAC
CTTTTCTTTACCCAAGGATTTTTAAAACTCAGATTTAAAACAAGGGGTTACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCA
GAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAGACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAA
TTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTTGGACATTATTTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGT
AACTGAAGCATAAACATCACATGGCATGTTTTGTCATTGTTTTCAGGTACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTG
TAACATTTACGAATTATTTTTTTAAACTTCAGTTTTACTGCATTTTCACAACATATCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTG
CTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
TLAMAKLCAEALLAALN*KHPWGPKEVFAHLIKELQDGIGQRQTVVRTLNATGEEIIQQSSKTDASI
LQEKLGSLNLRWQEVCKQLSDRKKRLEEQKNIL
SEFQRDLNEFVLWLEEADNIASIPLEPGKEQQL
KEKLEQVKLLVEELPLRQGILKQLNETGGPVLVS
APISPEEQDKLENKLKQTNLQWIKVSRALPEKQ
GEIEAQIKDLGQLEKKLEDLEEQLNHLLLWLSP
IRNQLEIYNQPNQEGPFDVKETEIAVQAKQPDVE
EILSKGQHLYKEKPATQPVKRKLEDLSSEWKAV
NRLLQELRAKQPDLAPGLTTIGASPTQTVTLVT
QPVVTKETAISKLEMPSSLMLEVPALADFNRAWT
ELTDWLSLLDQVIKSQRVMVGDLEDINEMIIKQ
KATMQDLEQRRPQLEELITAAQNLKNKTSNQEA
RTIITDRIERIQNQWDEVQEHLQNRRQQLNEMLK
DSTQWLEAKEEAEQVLGQARAKLESWKEGPYTV
DAIQKKITETKQLAKDLRQWQTNVDVANDLALK
LLRDYSADDTRKVHMITENINASWRSIHKRVSER
EAALEETHRLLQQFPLDLEKFLAWLTEAETTAN
VLQDATRKERLLEDSKGVKELMKQWQDLQGEIE
AHTDVYHNLDENSQKILRSLEGSDDAVLLQRRLD
NMNFKWSELRKKSLNIRSHLEASSDQWKRLHLS
LQELLVWLQLKDDELSRQAPIGGDFPAVQKQND
VHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEG
LEKLYQEPRELPPEERAQNVTRLLRKQAEEVNT
EWEKLNLHSADWQRKIDETLERLRELQEATDEL
DLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKA
LRGEIAPLKENVSHVNDLARQLTTLGIQLSPYN
LSTLEDLNTRWKLLQVAVEDRVRQLHEAHRDFG
PASQHFLSTSVQGPWERAISPNKVPYYINHETQT
TCWDHPKMTELYQSLADLNNVRFSAYRTAMKLR
RLQKALCLDLLSLSAACDALDQHNLKQNDQPMD
ILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLN
WLLNVYDTGRTGRIRVLSFKTGIISLCKAHLED
KYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPR
QLGEVASFGGSNIEPSVRSCFQFANNKPEIEAAL
FLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKC
NICKECPIIGFRYRSLKHFNYDICQSCFFSGRV
AKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKF
RTKRYFAKHPRMGYLPVQTVLEGDNMETPASSP
QLSHDDTHSRIEHYASRLAEMENSNGSYLNDSI
SPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQ
ILISLESEERGELERILADLEEENRNLQAEYDR
LKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELI
AEAKLLRQHKGRLEARMQILEDHNKQLESQLHRL
RQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQP
MLLRVVGSQTSDSMGEEDLLSPPQDTSTGLEEV
MEQLNNSFPSSRGHNVGSLFHMADDLGRAMESLV
SVMTDEEGAE*MFYNS*FPHGFYNIHTTKRIRQ
*EFTRNKSIFL*RVVVLYCRFQ*FLSLLLFC*Q
WQVLHVYAIVQKSYKKTTCKILIAK*LAISLYGT
HFGLFKNL*QL*RKIVN*SVLYKKKLFIKTPKN
KTNTHTHTYTHTHKTLRQRIVLHPFGVISI*NS
WLFLFLHIKDKTSSTTTPNDYYTLLI*ELSAEWG
RLEFSFHISICL*VYKYYSYIDKEIRISID*LF
PFFKCSCHILIERNYF*SVIQAYLLGLEWIFPG
AGSQEETTPH*NIVYSSRCFSF*TTFH*QRK*SK
VLDFFKGNM*MNTQDLLYQSE*SVGWLID*LID
TFSFLLLAMPRFRFNDASVEINQKVF*PCEHQK
VFFNSQAVAGR**GWRAMDSQPIPVKE*ATL*VK
DWMIVHNT*SSL*LQLNYYALFSQSKGTGWFGF
CCFFRFIVPCGMSF*MPQDII*NK*TLGKGVKQ
*PHHICDTDRYQPRSP*TVFPSFAFLCE*FHTGL
*VSKKEGKLIQMLQKNPSWWIRQVKYINKQTKI
AQKRGEKLKRKS*GLVGKSFTLSCHFISF*FLN
HSFNRYHRVTYNFANLLPLTSSVISFWRVG*HQV
*LAFGEWVLSIINN*LINIKHGFSCYFYLTLVL
GCS**LCTPEFTASPLVHCVIFFFLYNSFFFLH
NFQKKTQSSKVTNYQIT*RFGFCLAFFSFM*RWT
FSLPKDF*NSDLKQGVTLHPTKKFK*VSFILKS
EVNRVHK*FCFNLFVFLLDTLALE*VCHNI*TK
IESFIAAF*A*LIWTLFRVVFFITTKY*TVNHNV
TEA*TSHGMFCHCFQVLSSYLSIIIYCVLTPTL
*HLRIIFLNFSFTAFSQHIRLHQIYALLLYYST
ALLCISIKHAVML
Frame 1
H*QWQSFVRRHCWLL*TKSIRGDRKRFLHTLLRNSRMALGSGKLLSEH*MQLGKK*FSNPQKQMPV
FYRKNWEA*ICGGRRSANSCQTEKRG*KNKRISC
QNFKEI*MNLFYGWRKQITLLVSHLNLEKSSN*
KKSLSKSSYWWKSCPCAREFSNN*MKLEDPCL*
VLP*AQKSKINLKISSSRQISSG*RFPELYLRNK
EKLKLK*KTLGSLKKSLKTLKSS*IICCCGYLL
LGISWKFITNQTKKDHLTLRKLK*QFKLNNRMW
KRFCLKGSICTRKNQPLSQ*RGS*KI*ALSGRR*
TVYFKS*GQSSLT*LLD*PLLEPLLLRLLLW*H
NLWLLRKLPSPN*KCHLP*CWRYLLWQISTGLG
QNLPTGFLCLIKL*NHRG*WWVTLRISTR*SSSR
RQQCRIWNRGVPSWKNSLPLPKI*KTRPAIKRL
EQSLRIELKEFRISGMKYKNTFRTGGNS*MKC*
RIQHNGWKLRKKLSRS*DRPEPSLSHGRRVPIQ*
MQSKRKSQKPSSWPKTSASGRQM*MWQMTWP*N
FSGIILQMIPEKST**QRISMPLGEAFIKG*VS
ERLLWKKLIDYCNSSPWTWKSFLPGLQKLKQLPM
SYRMLPVRKGS*KTPRE*KS**NNGKTSKVKLK
LTQMFITTWMKTAKKS*DPWKVPMMQSCYKDVW
IT*TSSGVNFGKSLSTLGPIWKPVLTSGSVCTFL
CRNFWCGYS*KMMN*AGRHLLEATFQQFRSRTM
YIGPSRGN*KLKNL*S*VLLRLYEYF*QSSLWK
D*RNSTRSPESCLLRREPRMSLGFYESRLRRSIL
SGKN*TCTPLTGREK*MRPLKDSGNFKRPRMSW
TSSCAKLR*SRDPGSPWAISSLTLSKITSRKSR
HFEEKLRL*KRT*ATSMTLLASLPLWAFSSHRIT
SALWKT*TPDGSFCRWPSRTESGSCMKPTGTLV
QHLSTFFPRLSRVPGREPSRQTKCPTISTTRLK
QLAGTIPK*QSSTSL*LT*IMSDSQLIGLP*NSE
DCRRPFAWIS*ACQLHVMPWTSTTSSKMTSPWI
SCRLLIV*PLFMTAWSKSTTIWSTSLSAWICV*
TGC*MFMIRDEQGGSVSCLLKLASFPCVKHIWKT
STDTFSSKWQVQQDFVTSAGWASFCMILSKFQD
SWVKLHPLGAVTLSQVSGAASNLLIISQRSKRP
SS*TG*DWNPSPWCGCPSCTEWLLQKLPSIRPNV
TSAKSVQSLDSGTGV*STLIMTSAKAAFFLVEL
QKAIKCTIPWWNIALRLHQEKMFETLPRY*KTN
FEPKGILRSIPEWATCQCRLS*RGTTWKRLPRPL
SFHTMILIHALNIMLAG*QKWKTAMDLI*MIAS
LLMRA*MMNIC*SSITAKV*TRTPP*ASLVVLP
RS*FP*RVRKEGS*RES*QILRKKTGICKQNMTV
*SSSTNIKACPHCRPLLK*CPPLPRVPGMLSSL
LRPSYCVNTKAAWKPGCKSWKTTINSWSHSYTG
*GSCWSNPRQRPK*MAQRCPLLLPLYRGPTAVSL
CCSEWLAVKLRTPWVRKIFSVLPRTQAQG*RR*
WSNSTTPSLVQEDTM*EVFSTWQMIWAERWSP*
YQS*QMKKEQNKCFTTPDSRMVFIIFIQQRGLDS
KSLQEINLYFCEG*WYYTVDFSSF*VCYCFVNN
GRFYTSMQLYKKVIRKLHVKS**LNNLPFLYME
RILGCLKIYNSYKERL*TKVCFIKKSCL*KPLKT
KQTHTHTHTHTHTKL*GSALFCILLA*YPYEIH
GFFFFCILKIRLPLPPHQMTTTHCSFENCQLSG
AGLSFHFIYLYVYKYINTIVI*IKRYEFL*TDFF
HFLNVHVTS**KEITSSQSSRLTCLV*NGFFPE
PEARRKLHHTKTLSTAPDVSHFKQLSTDNESKV
KYWIFLKGTCE*IHRTYYIRVSNRLVG*LID*LI
HSASCC*QCHDLDLMMLQWKSIRRYSDLVNIRR
YFLTPKQ*QDDDRAGGLWIPSPSL*RSRPLFK*
RIG*LFIIHKVLCNYN*IIMPSSHSQKELGGLVF
VAFLDLLSHVG*VFKCHKT*FKINKLWEKV*NS
SPITFVILTGINPEAHELCFHPLHFSASSSTQV
CK*VRKKAN*FKCYKKTLLGGLDRLNI*TNKQKL
LKKEEKSSRGKAKDW*EKALLFHAILFLFDF*I
IHSIDTTV*PIILQICYL*HQV*LAFGEWADIK
CN*LLESGFCPLLIIN*LTSNTASHAISTSLWFW
GVPDNCAHLSSQLHHLSIALFSFSFIILSFSFI
IFKRKPKALR*QITKLHEDLVFVLHFFPLCDAG
PFLYPRIFKTQI*NKGLLYILLRSLSK*VSF*NQ
R*IECINNFVLIFLFFF*TH*LWSESVIIFEQK
LRALLLHFKHN*FGHYFVLCSL*PPSIKL*IIM
*LKHKHHMACFVIVFRY*VLT*VS*YIVF*HQHC
NIYELFF*TSVLLHFHNISDFTKYMPYYCIIVL
LYCVSQ*STQLCY
Frame 2
TSNGKALCGGIAGCSELKASVGTERGFCTPY*GTPGWHWAAANCCQNIECNWGRNNSAILKNRCQY
STGKIGKPESAVAGGLQTAVRQKKEARRTKEYL
VRISKRFK*ICFMVGGSR*HC*YPT*TWKRAATK
RKA*ASQVTGGRVAPAPGNSQTIK*NWRTRACK
CSHKPRRAR*T*K*AQADKSPVDKGFQSFT*ET
RRN*SSNKRPWAA*KKA*RP*RAVKSSAAVVISY
*ESVGNL*PTKPRRTI*R*GN*NSSSS*TTGCG
RDFV*RAAFVQGKTSHSASEEEVRRSEL*VEGG
KPFTSRAEGKAA*PSSWTDHYWSLSYSDCYSGDT
TCGY*GNCHLQTRNAIFLDVGGTCSGRFQPGLD
RTYRLAFSA*SSYKITEGDGG*P*GYQRDDHQA
EGNNAGFGTEASPVGRTHYRCPKFEKQDQQSRG*
NNHYGSN*KNSESVG*STRTPSEPEATVE*NVK
GFNTMAGS*GRS*AGLRTGQSQA*VMEGGSLYS
RCNPKENHRNQAVGQRPPPVADKCRCGK*LGPET
SPGLFCR*YQKSPHDNREYQCLLEKHS*KGE*A
RGCFGRNS*ITATVPPGPGKVSCLAYRS*NNCQ
CPTGCYP*GKAPRRLQGSKRADETMARPPR*N*S
SHRCLSQPG*KQPKNPEIPGRFR*CSPVTKTFG
*HELQVE*TSEKVSQH*VPFGSQF*PVEASAPF
SAGTSGVATAER**IKPAGTYWRRLSSSSEAERC
T*GLQEGIEN*RTCNHEYS*DCTNISDRAAFGR
TRETLPGAQRAAS*GESPECHSASTKAG*GGQY
*VGKIEPALR*LAEKNR*DP*KTPGTSRGHG*AG
PQAAPS*GDQGILAARGRSPH*LSPRSPRESQG
TSRRNCASEREREPRQ*PCSPAYHFGHSALTV*
PQHSGRPEHQMEASAGGRRGPSQAAA*SPQGLWS
SISALSFHVCPGSLGESHLAKQSALLYQPRDSN
NLLGPSQNDRALPVFS*PE*CQILSL*DCHETP
KTAEGPLLGSLEPVSCM*CLGPAQPQAK*PAHGY
PADY*LFDHYL*PPGARAQQFGQRPSLRGYVSE
LAAECL*YGTNREDPCPVF*NWHHFPV*STFGR
QVQIPFQASGKFNRIL*PAQAGPPSA*FYPNSKT
VG*SCILWGQ*H*AKCPELLPIC***ARDRSGP
LPRLDETGTPVHGVAARPAQSGCCRNCQASGQM
*HLQRVSNHWIQVQESKAL*L*HLPKLLFFWSSC
KRP*NALSHGGILHSDYIRRRCSRLCQGTKKQI
SNQKVFCEASPNGLPASADCLRGGQHGNACLVP
SAFTR*YSFTH*TLC*QASRNGKQQWILSK**HL
S**EHR**TFVNPALLPKFEPGLPPEPAS*SCP
DLDFLRE*GKRGARENPSRS*GRKQESASRI*P
SKAAART*RPVPTAVPS*NDAHLSPESPGC*AHC
*GQATASTQRPPGSQDANPGRPQ*TAGVTVTQA
KAAAGATPGRGQSEWHNGVLSFYLSTEVRQQSA
YAAPSGWQSNFGLHG*GRSSQSSPGHKHRVRGGD
GATQQLLP*FKRTQCRKSFPHGR*FGQSDGVLS
ISHDR*RRSRINVLQLLIPAWFL*YSYNKED*T
VRVYKK*IYIFVKGSGIIL*ISVVSKSVIVLLTM
AGFTRLCNCTKKL*ENYM*NLDS*ITCHFFIWN
AFWVV*KFITVIKKDCKLKCAL*KKVVYKNP*K
QNKHTHTHIHTHTQNFEAAHCFASFWRDIHMKFM
AFSFFAY*R*DFLYHHTK*LLHTAHLRTVS*VG
QA*VFISYIYMSISI*IL*LYR*RDTNFYRLTF
SIF*MFMSHPNRKKLLLVSHPGLPAWSRMDFSRS
RKPGGNYTTLKHCLQLQMFLILNNFPLTTKVK*
SIGFF*REHVNEYTGLIISE*VIGWLVD*LID*
YIQLPAASNATI*I**CFSGNQSEGILTL*TSEG
IF*LPSSSRTMIGLEGYGFPAHPCEGVGHSLSE
GLDDCS*YIKFSVITTKLLCPLLTVKRNWVVWF
LLLF*IYCPMWDEFLNATRHNLK*INFGKRCKTV
APSHL*Y*QVSTQKPMNCVSILCISLRVVPHRF
VSK*ERRQIDSNVTKKPFLVD*TG*IYKQTNKN
CSKKRRKAQEEKLRTGRKKLYSFMPFYFFLIFKS
FIQ*IPPCDL*FCKSVTSDIKCN*LLESGLTSS
VISFWRVGFVHY**LIN*HQTRLLMLFLPHFGF
GVFLIIVHT*VHSFTTCPLRYFLFPL*FFLFPS*
FSKENPKL*GNKLPNYMKIWFLSCIFFLYVTLD
LFFTQGFLKLRFKTRGYFTSY*EV*VSKFHSKI
RGK*SA*IILF*SFCFSFRHISSGVSLS*YLNKN
*ELYCCILSIINLDIISCCVLYNHQVLNCKS*C
N*SINITWHVLSLFSGTEFLLEYHNILCFNTNT
VTFTNYFFKLQFYCIFTTYQTSPNICLTIVL*YC
FTVYLNKARSYV
Sequence NM_004016
ACTTTCGGGGAGCCCGGCGGCTCTGGGAAGCTCACTCCTCCACTCGTACCCACACTCGACCGCGGAGCCCTTGCAGCCATGAGGGAACAGCTCAAAGGCCACGAGACTCAAACAACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGC
CATGAAACTCCGAAGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGAC
CAGCCCATGGATATCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGG
ATATGTGTCTGAACTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGC
ACATTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATC
CAAATTCCAAGACAGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGA
TCGAAGCGGCCCTCTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCA
TCAGGCCAAATGTAACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTT
TCTGGTCGAGTTGCAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTAC
TAAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCC
CGTTACTCTGATCAACTTCTGGCCAGTAGATTCTGCGCCTGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGC
AGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATCTAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACT
GCCAAAGTTTGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAAT
CCTAGCAGATCTTGAGGAAGAAAACAGGAATCTGCAAGCAGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCT
CCTGAAATGATGCCCACCTCTCCCCAGAGTCCCCGGGATGCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGA
TGCAAATCCTGGAAGACCACAATAAACAGCTGGAGTCACAGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCAC
AACGGTGTCCTCTCCTTCTACCTCTCTACAGAGGTCCGACAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAG
GAAGATCTTCTCAGTCCTCCCCAGGACACAAGCACAGGGTTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGACACAATGTAG
GAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGAGCAGAATAAATGTTTTACAACTC
CTGATTCCCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTATATTTTTGTGAAGGGTAGTGGTA
TTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTTATAAGAAAA
CTACATGTAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAGAAAGATTGT
AAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACATACACACACACACACAAAACTTTG
AGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAAAGATAAGACTTCCTCTACCACCA
CACCAAATGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATAAGTATATAA
ATACTATAGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGAAATTACTTC
TAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACCACACTAAAACATTGTCTACAGCT
CCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAACATGTGAATGAATACACAGGACTT
ATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGATTTAATGAT
GCTTCAGTGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGGGCTGGAGGG
CTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACATAAAGTTCTCTGTAATTACAACT
AAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCCCATGTGGGATGAGTTTTTAAATG
CCACAAGACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGGTATCAACCCAGAAGCCCAT
GAACTGTGTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTTACAAAAAAA
CCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCAAGAGGAAAAGCTAAGGACTGGTA
GGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACCGTGTGACCTATAATTTTGCAAATCTGT
TACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAATAATTAATT
AATTAACATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCTTCACCACTT
GTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTCTAAGGTAACAAATTACCAAATTA
CATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAAAACTCAGATTTAAAACAAGGGGT
TACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTTTTTGTTTTT
CTTTTAGACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTTGGACATTAT
TTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGCATGTTTTGTCATTGTTTTCAGGT
ACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTTTTAAACTTCAGTTTTACTGCATTTTCA
CAACATATCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
TFGEPGGSGKLTPPLVPTLDRGALAAMREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTA
MKLRRLQKALCLDLLSLSAACDALDQHNLKQND
QPMDILQIINCLTTIYDRLEQEHNNLVNVPLCV
DMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKA
HLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSI
QIPRQLGEVASFGGSNIEPSVRSCFQFANNKPE
IEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKH
QAKCNICKECPIIGFRYRSLKHFNYDICQSCFF
SGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKV
LKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETP
VTLINFWPVDSAPASSPQLSHDDTHSRIEHYAS
RLAEMENSNGSYLNDSISPNESIDDEHLLIQHY
CQSLNQDSPLSQPRSPAQILISLESEERGELERI
LADLEEENRNLQAEYDRLKQQHEHKGLSPLPSP
PEMMPTSPQSPRDAELIAEAKLLRQHKGRLEAR
MQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGT
TVSSPSTSLQRSDSSQPMLLRVVGSQTSDSMGE
EDLLSPPQDTSTGLEEVMEQLNNSFPSSRGHNV
GSLFHMADDLGRAMESLVSVMTDEEGAE*MFYNS
*FPHGFYNIHTTKRIRQ*EFTRNKSIFL*RVVV
LYCRFQ*FLSLLLFC*QWQVLHVYAIVQKSYKK
TTCKILIAK*LAISLYGTHFGLFKNL*QL*RKIV
N*SVLYKKKLFIKTPKNKTNTHTHTYTHTHKTL
RQRIVLHPFGVISI*NSWLFLFLHIKDKTSSTT
TPNDYYTLLI*ELSAEWGRLEFSFHISICL*VYK
YYSYIDKEIRISID*LFPFFKCSCHILIERNYF
*SVIQAYLLGLEWIFPGAGSQEETTPH*NIVYS
SRCFSF*TTFH*QRK*SKVLDFFKGNM*MNTQDL
LYQSE*SVGWLID*LIDTFSFLLLAMPRFRFND
ASVEINQKVF*PCEHQKVFFNSQAVAGR**GWR
AMDSQPIPVKE*ATL*VKDWMIVHNT*SSL*LQL
NYYALFSQSKGTGWFGFCCFFRFIVPCGMSF*M
PQDII*NK*TLGKGVKQ*PHHICDTDRYQPRSP
*TVFPSFAFLCE*FHTGL*VSKKEGKLIQMLQKN
PSWWIRQVKYINKQTKIAQKRGEKLKRKS*GLV
GKSFTLSCHFISF*FLNHSFNRYHRVTYNFANL
LPLTSSVISFWRVG*HQV*LAFGEWVLSIINN*L
INIKHGFSCYFYLTLVLGCS**LCTPEFTASPL
VHCVIFFFLYNSFFFLHNFQKKTQSSKVTNYQI
T*RFGFCLAFFSFM*RWTFSLPKDF*NSDLKQGV
TLHPTKKFK*VSFILKSEVNRVHK*FCFNLFVF
LLDTLALE*VCHNI*TKIESFIAAF*A*LIWTL
FRVVFFITTKY*TVNHNVTEA*TSHGMFCHCFQV
LSSYLSIIIYCVLTPTL*HLRIIFLNFSFTAFS
QHIRLHQIYALLLYYSTALLCISIKHAVML
Frame 1
LSGSPAALGSSLLHSYPHSTAEPLQP*GNSSKATRLKQLAGTIPK*QSSTSL*LT*IMSDSQLIGL
P*NSEDCRRPFAWIS*ACQLHVMPWTSTTSSKMT
SPWISCRLLIV*PLFMTAWSKSTTIWSTSLSAW
ICV*TGC*MFMIRDEQGGSVSCLLKLASFPCVK
HIWKTSTDTFSSKWQVQQDFVTSAGWASFCMILS
KFQDSWVKLHPLGAVTLSQVSGAASNLLIISQR
SKRPSS*TG*DWNPSPWCGCPSCTEWLLQKLPS
IRPNVTSAKSVQSLDSGTGV*STLIMTSAKAAFF
LVELQKAIKCTIPWWNIALRLHQEKMFETLPRY
*KTNFEPKGILRSIPEWATCQCRLS*RGTTWKL
PLL*STSGQ*ILRLPRPLSFHTMILIHALNIMLA
G*QKWKTAMDLI*MIASLLMRA*MMNIC*SSIT
AKV*TRTPP*ASLVVLPRS*FP*RVRKEGS*RE
S*QILRKKTGICKQNMTV*SSSTNIKACPHCRPL
LK*CPPLPRVPGMLSSLLRPSYCVNTKAAWKPG
CKSWKTTINSWSHSYTG*GSCWSNPRQRPK*MA
QRCPLLLPLYRGPTAVSLCCSEWLAVKLRTPWVR
KIFSVLPRTQAQG*RR*WSNSTTPSLVQEDTM*
EVFSTWQMIWAERWSP*YQS*QMKKEQNKCFTT
PDSRMVFIIFIQQRGLDSKSLQEINLYFCEG*WY
YTVDFSSF*VCYCFVNNGRFYTSMQLYKKVIRK
LHVKS**LNNLPFLYMERILGCLKIYNSYKERL
*TKVCFIKKSCL*KPLKTKQTHTHTHTHTHTKL*
GSALFCILLA*YPYEIHGFFFFCILKIRLPLPP
HQMTTTHCSFENCQLSGAGLSFHFIYLYVYKYI
NTIVI*IKRYEFL*TDFFHFLNVHVTS**KEITS
SQSSRLTCLV*NGFFPEPEARRKLHHTKTLSTA
PDVSHFKQLSTDNESKVKYWIFLKGTCE*IHRT
YYIRVSNRLVG*LID*LIHSASCC*QCHDLDLMM
LQWKSIRRYSDLVNIRRYFLTPKQ*QDDDRAGG
LWIPSPSL*RSRPLFK*RIG*LFIIHKVLCNYN
*IIMPSSHSQKELGGLVFVAFLDLLSHVG*VFKC
HKT*FKINKLWEKV*NSSPITFVILTGINPEAH
ELCFHPLHFSASSSTQVCK*VRKKAN*FKCYKK
TLLGGLDRLNI*TNKQKLLKKEEKSSRGKAKDW*
EKALLFHAILFLFDF*IIHSIDTTV*PIILQIC
YL*HQV*LAFGEWADIKCN*LLESGFCPLLIIN
*LTSNTASHAISTSLWFWGVPDNCAHLSSQLHHL
SIALFSFSFIILSFSFIIFKRKPKALR*QITKL
HEDLVFVLHFFPLCDAGPFLYPRIFKTQI*NKG
LLYILLRSLSK*VSF*NQR*IECINNFVLIFLFF
F*TH*LWSESVIIFEQKLRALLLHFKHN*FGHY
FVLCSL*PPSIKL*IIM*LKHKHHMACFVIVFR
Y*VLT*VS*YIVF*HQHCNIYELFF*TSVLLHFH
NISDFTKYMPYYCIIVLLYCVSQ*STQLCY
Frame 2
FRGARRLWEAHSSTRTHTRPRSPCSHEGTAQRPRDSNNLLGPSQNDRALPVFS*PE*CQILSL*DC
HETPKTAEGPLLGSLEPVSCM*CLGPAQPQAK*
PAHGYPADY*LFDHYL*PPGARAQQFGQRPSLRG
YVSELAAECL*YGTNREDPCPVF*NWHHFPV*S
TFGRQVQIPFQASGKFNRIL*PAQAGPPSA*FY
PNSKTVG*SCILWGQ*H*AKCPELLPIC***ARD
RSGPLPRLDETGTPVHGVAARPAQSGCCRNCQA
SGQM*HLQRVSNHWIQVQESKAL*L*HLPKLLF
FWSSCKRP*NALSHGGILHSDYIRRRCSRLCQGT
KKQISNQKVFCEASPNGLPASADCLRGGQHGNS
RYSDQLLASRFCACLVPSAFTR*YSFTH*TLC*
QASRNGKQQWILSK**HLS**EHR**TFVNPALL
PKFEPGLPPEPAS*SCPDLDFLRE*GKRGAREN
PSRS*GRKQESASRI*PSKAAART*RPVPTAVP
S*NDAHLSPESPGC*AHC*GQATASTQRPPGSQD
ANPGRPQ*TAGVTVTQAKAAAGATPGRGQSEWH
NGVLSFYLSTEVRQQSAYAAPSGWQSNFGLHG*
GRSSQSSPGHKHRVRGGDGATQQLLP*FKRTQCR
KSFPHGR*FGQSDGVLSISHDR*RRSRINVLQL
LIPAWFL*YSYNKED*TVRVYKK*IYIFVKGSG
IIL*ISVVSKSVIVLLTMAGFTRLCNCTKKL*EN
YM*NLDS*ITCHFFIWNAFWVV*KFITVIKKDC
KLKCAL*KKVVYKNP*KQNKHTHTHIHTHTQNF
EAAHCFASFWRDIHMKFMAFSFFAY*R*DFLYHH
TK*LLHTAHLRTVS*VGQA*VFISYIYMSISI*
IL*LYR*RDTNFYRLTFSIF*MFMSHPNRKKLL
LVSHPGLPAWSRMDFSRSRKPGGNYTTLKHCLQL
QMFLILNNFPLTTKVK*SIGFF*REHVNEYTGL
IISE*VIGWLVD*LID*YIQLPAASNATI*I**
CFSGNQSEGILTL*TSEGIF*LPSSSRTMIGLEG
YGFPAHPCEGVGHSLSEGLDDCS*YIKFSVITT
KLLCPLLTVKRNWVVWFLLLF*IYCPMWDEFLN
ATRHNLK*INFGKRCKTVAPSHL*Y*QVSTQKPM
NCVSILCISLRVVPHRFVSK*ERRQIDSNVTKK
PFLVD*TG*IYKQTNKNCSKKRRKAQEEKLRTG
RKKLYSFMPFYFFLIFKSFIQ*IPPCDL*FCKSV
TSDIKCN*LLESGLTSSVISFWRVGFVHY**LI
N*HQTRLLMLFLPHFGFGVFLIIVHT*VHSFTT
CPLRYFLFPL*FFLFPS*FSKENPKL*GNKLPNY
MKIWFLSCIFFLYVTLDLFFTQGFLKLRFKTRG
YFTSY*EV*VSKFHSKIRGK*SA*IILF*SFCF
SFRHISSGVSLS*YLNKN*ELYCCILSIINLDII
SCCVLYNHQVLNCKS*CN*SINITWHVLSLFSG
TEFLLEYHNILCFNTNTVTFTNYFFKLQFYCIF
TTYQTSPNICLTIVL*YCFTVYLNKARSYV
Sequence NM_004010
ATGCTGTCTGTGAAGCTGAATCTGTGAGAACACCTCACTATTCACGGCAACCGGAGTGGAAGAAACAGGTGCAAAAAGATTGTGTGTTTGTCTGCTTTTGTGAGGCTGGTCAGAGATTCTGTGCCTGCTTTATCTGTGCTTGGCTATGACTCTACCTCCAGGTTTACCATACCCCATAGAATGTGTAAGAGAAAAGTACC
AACAGGGAAATCAGCAAAAAGCTTTCCTATGAAGGTGTGTAGCCAGCCTCCGCAGAATTTGAAATGTCTGAGGTCTCATCTGGTGAGTAAAAGCTGCAGA
TAAATGCAACAGCCATTCAGAAGAATGATAAATTCCACAAGCATTCAGAAGCAGGCTTCCCTAAAGATGAAAGAGAAGATGTTCAAAAGAAAACATTCAC
AAAATGGGTAAATGCACAATTTTCTAAGTTTGGGAAGCAGCATATTGAGAACCTCTTCAGTGACCTACAGGATGGGAGGCGCCTCCTAGACCTCCTCGAA
GGCCTGACAGGGCAAAAACTGCCAAAAGAAAAAGGATCCACAAGAGTTCATGCCCTGAACAATGTCAACAAGGCACTGCGGGTTTTGCAGAACAATAATG
TTGATTTAGTGAATATTGGAAGTACTGACATCGTAGATGGAAATCATAAACTGACTCTTGGTTTGATTTGGAATATAATCCTCCACTGGCAGGTCAAAAA
TGTAATGAAAAATATCATGGCTGGATTGCAACAAACCAACAGTGAAAAGATTCTCCTGAGCTGGGTCCGACAATCAACTCGTAATTATCCACAGGTTAAT
GTAATCAACTTCACCACCAGCTGGTCTGATGGCCTGGCTTTGAATGCTCTCATCCATAGTCATAGGCCAGACCTATTTGACTGGAATAGTGTGGTTTGCC
AGCAGTCAGCCACACAACGACTGGAACATGCATTCAACATCGCCAGATATCAATTAGGCATAGAGAAACTACTCGATCCTGAAGATGTTGATACCACCTA
TCCAGATAAGAAGTCCATCTTAATGTACATCACATCACTCTTCCAAGTTTTGCCTCAACAAGTGAGCATTGAAGCCATCCAGGAAGTGGAAATGTTGCCA
AGGCCACCTAAAGTGACTAAAGAAGAACATTTTCAGTTACATCATCAAATGCACTATTCTCAACAGATCACGGTCAGTCTAGCACAGGGATATGAGAGAA
CTTCTTCCCCTAAGCCTCGATTCAAGAGCTATGCCTACACACAGGCTGCTTATGTCACCACCTCTGACCCTACACGGAGCCCATTTCCTTCACAGCATTT
GGAAGCTCCTGAAGACAAGTCATTTGGCAGTTCATTGATGGAGAGTGAAGTAAACCTGGACCGTTATCAAACAGCTTTAGAAGAAGTATTATCGTGGCTT
CTTTCTGCTGAGGACACATTGCAAGCACAAGGAGAGATTTCTAATGATGTGGAAGTGGTGAAAGACCAGTTTCATACTCATGAGGGGTACATGATGGATT
TGACAGCCCATCAGGGCCGGGTTGGTAATATTCTACAATTGGGAAGTAAGCTGATTGGAACAGGAAAATTATCAGAAGATGAAGAAACTGAAGTACAAGA
GCAGATGAATCTCCTAAATTCAAGATGGGAATGCCTCAGGGTAGCTAGCATGGAAAAACAAAGCAATTTACATAGAGTTTTAATGGATCTCCAGAATCAG
AAACTGAAAGAGTTGAATGACTGGCTAACAAAAACAGAAGAAAGAACAAGGAAAATGGAGGAAGAGCCTCTTGGACCTGATCTTGAAGACCTAAAACGCC
AAGTACAACAACATAAGGTGCTTCAAGAAGATCTAGAACAAGAACAAGTCAGGGTCAATTCTCTCACTCACATGGTGGTGGTAGTTGATGAATCTAGTGG
AGATCACGCAACTGCTGCTTTGGAAGAACAACTTAAGGTATTGGGAGATCGATGGGCAAACATCTGTAGATGGACAGAAGACCGCTGGGTTCTTTTACAA
GACATCCTTCTCAAATGGCAACGTCTTACTGAAGAACAGTGCCTTTTTAGTGCATGGCTTTCAGAAAAAGAAGATGCAGTGAACAAGATTCACACAACTG
GCTTTAAAGATCAAAATGAAATGTTATCAAGTCTTCAAAAACTGGCCGTTTTAAAAGCGGATCTAGAAAAGAAAAAGCAATCCATGGGCAAACTGTATTC
ACTCAAACAAGATCTTCTTTCAACACTGAAGAATAAGTCAGTGACCCAGAAGACGGAAGCATGGCTGGATAACTTTGCCCGGTGTTGGGATAATTTAGTC
CAAAAACTTGAAAAGAGTACAGCACAGATTTCACAGGCTGTCACCACCACTCAGCCATCACTAACACAGACAACTGTAATGGAAACAGTAACTACGGTGA
CCACAAGGGAACAGATCCTGGTAAAGCATGCTCAAGAGGAACTTCCACCACCACCTCCCCAAAAGAAGAGGCAGATTACTGTGGATTCTGAAATTAGGAA
AAGGTTGGATGTTGATATAACTGAACTTCACAGCTGGATTACTCGCTCAGAAGCTGTGTTGCAGAGTCCTGAATTTGCAATCTTTCGGAAGGAAGGCAAC
TTCTCAGACTTAAAAGAAAAAGTCAATGCCATAGAGCGAGAAAAAGCTGAGAAGTTCAGAAAACTGCAAGATGCCAGCAGATCAGCTCAGGCCCTGGTGG
AACAGATGGTGAATGAGGGTGTTAATGCAGATAGCATCAAACAAGCCTCAGAACAACTGAACAGCCGGTGGATCGAATTCTGCCAGTTGCTAAGTGAGAG
ACTTAACTGGCTGGAGTATCAGAACAACATCATCGCTTTCTATAATCAGCTACAACAATTGGAGCAGATGACAACTACTGCTGAAAACTGGTTGAAAATC
CAACCCACCACCCCATCAGAGCCAACAGCAATTAAAAGTCAGTTAAAAATTTGTAAGGATGAAGTCAACCGGCTATCAGATCTTCAACCTCAAATTGAAC
GATTAAAAATTCAAAGCATAGCCCTGAAAGAGAAAGGACAAGGACCCATGTTCCTGGATGCAGACTTTGTGGCCTTTACAAATCATTTTAAGCAAGTCTT
TTCTGATGTGCAGGCCAGAGAGAAAGAGCTACAGACAATTTTTGACACTTTGCCACCAATGCGCTATCAGGAGACCATGAGTGCCATCAGGACATGGGTC
CAGCAGTCAGAAACCAAACTCTCCATACCTCAACTTAGTGTCACCGACTATGAAATCATGGAGCAGAGACTCGGGGAATTGCAGGCTTTACAAAGTTCTC
TGCAAGAGCAACAAAGTGGCCTATACTATCTCAGCACCACTGTGAAAGAGATGTCGAAGAAAGCGCCCTCTGAAATTAGCCGGAAATATCAATCAGAATT
TGAAGAAATTGAGGGACGCTGGAAGAAGCTCTCCTCCCAGCTGGTTGAGCATTGTCAAAAGCTAGAGGAGCAAATGAATAAACTCCGAAAAATTCAGAAT
CACATACAAACCCTGAAGAAATGGATGGCTGAAGTTGATGTTTTTCTGAAGGAGGAATGGCCTGCCCTTGGGGATTCAGAAATTCTAAAAAAGCAGCTGA
AACAGTGCAGACTTTTAGTCAGTGATATTCAGACAATTCAGCCCAGTCTAAACAGTGTCAATGAAGGTGGGCAGAAGATAAAGAATGAAGCAGAGCCAGA
GTTTGCTTCGAGACTTGAGACAGAACTCAAAGAACTTAACACTCAGTGGGATCACATGTGCCAACAGGTCTATGCCAGAAAGGAGGCCTTGAAGGGAGGT
TTGGAGAAAACTGTAAGCCTCCAGAAAGATCTATCAGAGATGCACGAATGGATGACACAAGCTGAAGAAGAGTATCTTGAGAGAGATTTTGAATATAAAA
CTCCAGATGAATTACAGAAAGCAGTTGAAGAGATGAAGAGAGCTAAAGAAGAGGCCCAACAAAAAGAAGCGAAAGTGAAACTCCTTACTGAGTCTGTAAA
TAGTGTCATAGCTCAAGCTCCACCTGTAGCACAAGAGGCCTTAAAAAAGGAACTTGAAACTCTAACCACCAACTACCAGTGGCTCTGCACTAGGCTGAAT
GGGAAATGCAAGACTTTGGAAGAAGTTTGGGCATGTTGGCATGAGTTATTGTCATACTTGGAGAAAGCAAACAAGTGGCTAAATGAAGTAGAATTTAAAC
TTAAAACCACTGAAAACATTCCTGGCGGAGCTGAGGAAATCTCTGAGGTGCTAGATTCACTTGAAAATTTGATGCGACATTCAGAGGATAACCCAAATCA
GATTCGCATATTGGCACAGACCCTAACAGATGGCGGAGTCATGGATGAGCTAATCAATGAGGAACTTGAGACATTTAATTCTCGTTGGAGGGAACTACAT
GAAGAGGCTGTAAGGAGGCAAAAGTTGCTTGAACAGAGCATCCAGTCTGCCCAGGAGACTGAAAAATCCTTACACTTAATCCAGGAGTCCCTCACATTCA
TTGACAAGCAGTTGGCAGCTTATATTGCAGACAAGGTGGACGCAGCTCAAATGCCTCAGGAAGCCCAGAAAATCCAATCTGATTTGACAAGTCATGAGAT
CAGTTTAGAAGAAATGAAGAAACATAATCAGGGGAAGGAGGCTGCCCAAAGAGTCCTGTCTCAGATTGATGTTGCACAGAAAAAATTACAAGATGTCTCC
ATGAAGTTTCGATTATTCCAGAAACCAGCCAATTTTGAGCAGCGTCTACAAGAAAGTAAGATGATTTTAGATGAAGTGAAGATGCACTTGCCTGCATTGG
AAACAAAGAGTGTGGAACAGGAAGTAGTACAGTCACAGCTAAATCATTGTGTGAACTTGTATAAAAGTCTGAGTGAAGTGAAGTCTGAAGTGGAAATGGT
GATAAAGACTGGACGTCAGATTGTACAGAAAAAGCAGACGGAAAATCCCAAAGAACTTGATGAAAGAGTAACAGCTTTGAAATTGCATTATAATGAGCTG
GGAGCAAAGGTAACAGAAAGAAAGCAACAGTTGGAGAAATGCTTGAAATTGTCCCGTAAGATGCGAAAGGAAATGAATGTCTTGACAGAATGGCTGGCAG
CTACAGATATGGAATTGACAAAGAGATCAGCAGTTGAAGGAATGCCTAGTAATTTGGATTCTGAAGTTGCCTGGGGAAAGGCTACTCAAAAAGAGATTGA
GAAACAGAAGGTGCACCTGAAGAGTATCACAGAGGTAGGAGAGGCCTTGAAAACAGTTTTGGGCAAGAAGGAGACGTTGGTGGAAGATAAACTCAGTCTT
CTGAATAGTAACTGGATAGCTGTCACCTCCCGAGCAGAAGAGTGGTTAAATCTTTTGTTGGAATACCAGAAACACATGGAAACTTTTGACCAGAATGTGG
ACCACATCACAAAGTGGATCATTCAGGCTGACACACTTTTGGATGAATCAGAGAAAAAGAAACCCCAGCAAAAAGAAGACGTGCTTAAGCGTTTAAAGGC
AGAACTGAATGACATACGCCCAAAGGTGGACTCTACACGTGACCAAGCAGCAAACTTGATGGCAAACCGCGGTGACCACTGCAGGAAATTAGTAGAGCCC
CAAATCTCAGAGCTCAACCATCGATTTGCAGCCATTTCACACAGAATTAAGACTGGAAAGGCCTCCATTCCTTTGAAGGAATTGGAGCAGTTTAACTCAG
ATATACAAAAATTGCTTGAACCACTGGAGGCTGAAATTCAGCAGGGGGTGAATCTGAAAGAGGAAGACTTCAATAAAGATATGAATGAAGACAATGAGGG
TACTGTAAAAGAATTGTTGCAAAGAGGAGACAACTTACAACAAAGAATCACAGATGAGAGAAAGCGAGAGGAAATAAAGATAAAACAGCAGCTGTTACAG
ACAAAACATAATGCTCTCAAGGATTTGAGGTCTCAAAGAAGAAAAAAGGCTCTAGAAATTTCTCATCAGTGGTATCAGTACAAGAGGCAGGCTGATGATC
TCCTGAAATGCTTGGATGACATTGAAAAAAAATTAGCCAGCCTACCTGAGCCCAGAGATGAAAGGAAAATAAAGGAAATTGATCGGGAATTGCAGAAGAA
GAAAGAGGAGCTGAATGCAGTGCGTAGGCAAGCTGAGGGCTTGTCTGAGGATGGGGCCGCAATGGCAGTGGAGCCAACTCAGATCCAGCTCAGCAAGCGC
TGGCGGGAAATTGAGAGCAAATTTGCTCAGTTTCGAAGACTCAACTTTGCACAAATTCACACTGTCCGTGAAGAAACGATGATGGTGATGACTGAAGACA
TGCCTTTGGAAATTTCTTATGTGCCTTCTACTTATTTGACTGAAATCACTCATGTCTCACAAGCCCTATTAGAAGTGGAACAACTTCTCAATGCTCCTGA
CCTCTGTGCTAAGGACTTTGAAGATCTCTTTAAGCAAGAGGAGTCTCTGAAGAATATAAAAGATAGTCTACAACAAAGCTCAGGTCGGATTGACATTATT
CATAGCAAGAAGACAGCAGCATTGCAAAGTGCAACGCCTGTGGAAAGGGTGAAGCTACAGGAAGCTCTCTCCCAGCTTGATTTCCAATGGGAAAAAGTTA
ACAAAATGTACAAGGACCGACAAGGGCGATTTGACAGATCTGTTGAGAAATGGCGGCGTTTTCATTATGATATAAAGATATTTAATCAGTGGCTAACAGA
AGCTGAACAGTTTCTCAGAAAGACACAAATTCCTGAGAATTGGGAACATGCTAAATACAAATGGTATCTTAAGGAACTCCAGGATGGCATTGGGCAGCGG
CAAACTGTTGTCAGAACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGATGCCAGTATTCTACAGGAAAAATTGGGAAGCCTGA
ATCTGCGGTGGCAGGAGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGGCTAGAAGAACAAAAGAATATCTTGTCAGAATTTCAAAGAGATTTAAATGA
ATTTGTTTTATGGTTGGAGGAAGCAGATAACATTGCTAGTATCCCACTTGAACCTGGAAAAGAGCAGCAACTAAAAGAAAAGCTTGAGCAAGTCAAGTTA
CTGGTGGAAGAGTTGCCCCTGCGCCAGGGAATTCTCAAACAATTAAATGAAACTGGAGGACCCGTGCTTGTAAGTGCTCCCATAAGCCCAGAAGAGCAAG
ATAAACTTGAAAATAAGCTCAAGCAGACAAATCTCCAGTGGATAAAGGTTTCCAGAGCTTTACCTGAGAAACAAGGAGAAATTGAAGCTCAAATAAAAGA
CCTTGGGCAGCTTGAAAAAAAGCTTGAAGACCTTGAAGAGCAGTTAAATCATCTGCTGCTGTGGTTATCTCCTATTAGGAATCAGTTGGAAATTTATAAC
CAACCAAACCAAGAAGGACCATTTGACGTTAAGGAAACTGAAATAGCAGTTCAAGCTAAACAACCGGATGTGGAAGAGATTTTGTCTAAAGGGCAGCATT
TGTACAAGGAAAAACCAGCCACTCAGCCAGTGAAGAGGAAGTTAGAAGATCTGAGCTCTGAGTGGAAGGCGGTAAACCGTTTACTTCAAGAGCTGAGGGC
AAAGCAGCCTGACCTAGCTCCTGGACTGACCACTATTGGAGCCTCTCCTACTCAGACTGTTACTCTGGTGACACAACCTGTGGTTACTAAGGAAACTGCC
ATCTCCAAACTAGAAATGCCATCTTCCTTGATGTTGGAGGTACCTGCTCTGGCAGATTTCAACCGGGCTTGGACAGAACTTACCGACTGGCTTTCTCTGC
TTGATCAAGTTATAAAATCACAGAGGGTGATGGTGGGTGACCTTGAGGATATCAACGAGATGATCATCAAGCAGAAGGCAACAATGCAGGATTTGGAACA
GAGGCGTCCCCAGTTGGAAGAACTCATTACCGCTGCCCAAAATTTGAAAAACAAGACCAGCAATCAAGAGGCTAGAACAATCATTACGGATCGAATTGAA
AGAATTCAGAATCAGTGGGATGAAGTACAAGAACACCTTCAGAACCGGAGGCAACAGTTGAATGAAATGTTAAAGGATTCAACACAATGGCTGGAAGCTA
AGGAAGAAGCTGAGCAGGTCTTAGGACAGGCCAGAGCCAAGCTTGAGTCATGGAAGGAGGGTCCCTATACAGTAGATGCAATCCAAAAGAAAATCACAGA
AACCAAGCAGTTGGCCAAAGACCTCCGCCAGTGGCAGACAAATGTAGATGTGGCAAATGACTTGGCCCTGAAACTTCTCCGGGATTATTCTGCAGATGAT
ACCAGAAAAGTCCACATGATAACAGAGAATATCAATGCCTCTTGGAGAAGCATTCATAAAAGGGTGAGTGAGCGAGAGGCTGCTTTGGAAGAAACTCATA
GATTACTGCAACAGTTCCCCCTGGACCTGGAAAAGTTTCTTGCCTGGCTTACAGAAGCTGAAACAACTGCCAATGTCCTACAGGATGCTACCCGTAAGGA
AAGGCTCCTAGAAGACTCCAAGGGAGTAAAAGAGCTGATGAAACAATGGCAAGACCTCCAAGGTGAAATTGAAGCTCACACAGATGTTTATCACAACCTG
GATGAAAACAGCCAAAAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTACAAAGACGTTTGGATAACATGAACTTCAAGTGGAGTGAAC
TTCGGAAAAAGTCTCTCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCGTCTGCACCTTTCTCTGCAGGAACTTCTGGTGTGGCTACA
GCTGAAAGATGATGAATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAGAAGCAGAACGATGTACATAGGGCCTTCAAGAGGGAATTG
AAAACTAAAGAACCTGTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGCAGCCTTTGGAAGGACTAGAGAAACTCTACCAGGAGCCCA
GAGAGCTGCCTCCTGAGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGAGGAGGTCAATACTGAGTGGGAAAAATTGAACCTGCACTC
CGCTGACTGGCAGAGAAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCCACGGATGAGCTGGACCTCAAGCTGCGCCAAGCTGAGGTG
ATCAAGGGATCCTGGCAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCGAGAAAGTCAAGGCACTTCGAGGAGAAATTGCGCCTCTGA
AAGAGAACGTGAGCCACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCTCTCACCGTATAACCTCAGCACTCTGGAAGACCTGAACAC
CAGATGGAAGCTTCTGCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCACAGGGACTTTGGTCCAGCATCTCAGCACTTTCTTTCCACG
TCTGTCCAGGGTCCCTGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACCACGAGACTCAAACAACTTGCTGGGACCATCCCAAAATGA
CAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGCCATGAAACTCCGAAGACTGCAGAAGGCCCTTTGCTTGGA
TCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGACCAGCCCATGGATATCCTGCAGATTATTAATTGTTTGACC
ACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGGATATGTGTCTGAACTGGCTGCTGAATGTTTATGATACGG
GACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGCACATTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGT
GGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATCCAAATTCCAAGACAGTTGGGTGAAGTTGCATCCTTTGGG
GGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGATCGAAGCGGCCCTCTTCCTAGACTGGATGAGACTGGAAC
CCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCATCAGGCCAAATGTAACATCTGCAAAGAGTGTCCAATCAT
TGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGTCGAGTTGCAAAAGGCCATAAAATGCACTATCCC
ATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTAAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGC
ATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCCCGTTACTCTGATCAACTTCTGGCCAGTAGATTCTGCGCC
TGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATCTA
AATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTTTGAACCAGGACTCCCCCCTGAGCCAGCCTC
GTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAAACAGGAATCTGCAAGC
AGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATGATGCCCACCTCTCCCCAGAGTCCCCGGGAT
GCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCCTGGAAGACCACAATAAACAGCTGGAGTCAC
AGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTCCTCTCCTTCTACCTCTCTACAGAGGTCCGA
CAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTTCTCAGTCCTCCCCAGGACACAAGCACAGGG
TTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCCCTGGAAAGCCAATGAGAGAGGACACAATGTAGGAAGTCT
TTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGAGCAGAATAAATGTTTTACAACTCCTGATTC
CCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTATATTTTTGTGAAGGGTAGTGGTATTATACT
GTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTTATAAGAAAACTACATG
TAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAGAAAGATTGTAAACTAA
AGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACATACACACACACACACAAAACTTTGAGGCAGC
GCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAAAGATAAGACTTCCTCTACCACCACACCAAA
TGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATAAGTATATAAATACTAT
AGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGAAATTACTTCTAGTCAG
TCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACCACACTAAAACATTGTCTACAGCTCCAGATG
TTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAACATGTGAATGAATACACAGGACTTATTATAT
CAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGATTTAATGATGCTTCAG
TGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGGGCTGGAGGGCTATGGA
TTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACATAAAGTTCTCTGTAATTACAACTAAATTAT
TATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCCCATGTGGGATGAGTTTTTAAATGCCACAAG
ACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGGTATCAACCCAGAAGCCCATGAACTGT
GTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTTACAAAAAAACCCTTCT
TGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCAAGAGGAAAAGCTAAGGACTGGTAGGAAAAA
GCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACCGTGTGACCTATAATTTTGCAAATCTGTTACCTCT
GACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAATAATTAATTAATTAAC
ATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCTTCACCACTTGTCCATT
GCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTCTAAGGTAACAAATTACCAAATTACATGAAG
ATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAAAACTCAGATTTAAAACAAGGGGTTACTTTA
CATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAG
ACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTTGGACATTATTTCGTGT
TGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGCATGTTTTGTCATTGTTTTCAGGTACTGAGT
TCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTTTTAAACTTCAGTTTTACTGCATTTTCACAACATA
TCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
MLSVKLNL*EHLTIHGNRSGRNRCKKIVCLSAFVRLVRDSVPALSVLGYDSTSRFTIPHRMCKRKVP
TGKSAKSFPMKVCSQPPQNLKCLRSHLVSKSCR
*MQQPFRRMINSTSIQKQASLKMKEKMFKRKHS
QNG*MHNFLSLGSSILRTSSVTYRMGGAS*TSSK
A*QGKNCQKKKDPQEFMP*TMSTRHCGFCRTIM
LI**ILEVLTS*MEIIN*LLV*FGI*SSTGRSK
M**KISWLDCNKPTVKRFS*AGSDNQLVIIHRLM
*STSPPAGLMAWL*MLSSIVIGQTYLTGIVWFA
SSQPHNDWNMHSTSPDIN*A*RNYSILKMLIPP
IQIRSPS*CTSHHSSKFCLNK*ALKPSRKWKCCQ
GHLK*LKKNIFSYIIKCTILNRSRSV*HRDMRE
LLPLSLDSRAMPTHRLLMSPPLTLHGAHFLHSI
WKLLKTSHLAVH*WRVK*TWTVIKQL*KKYYRGF
FLLRTHCKHKERFLMMWKW*KTSFILMRGT*WI
*QPIRAGLVIFYNWEVS*LEQENYQKMKKLKYK
SR*IS*IQDGNASG*LAWKNKAIYIEF*WISRIR
N*KS*MTG*QKQKKEQGKWRKSLLDLILKT*NA
KYNNIRCFKKI*NKNKSGSILSLTWWW*LMNLV
EITQLLLWKNNLRYWEIDGQTSVDGQKTAGFFYK
TSFSNGNVLLKNSAFLVHGFQKKKMQ*TRFTQL
ALKIKMKCYQVFKNWPF*KRI*KRKSNPWANCI
HSNKIFFQH*RISQ*PRRRKHGWITLPGVGII*S
KNLKRVQHRFHRLSPPLSHH*HRQL*WKQ*LR*
PQGNRSW*SMLKRNFHHHLPKRRGRLLWILKLG
KGWMLI*LNFTAGLLAQKLCCRVLNLQSFGRKAT
SQT*KKKSMP*SEKKLRSSENCKMPADQLRPWW
NRW*MRVLMQIASNKPQNN*TAGGSNSASC*VR
DLTGWSIRTTSSLSIISYNNWSR*QLLLKTG*KS
NPPPHQSQQQLKVS*KFVRMKSTGYQIFNLKLN
D*KFKA*P*KRKDKDPCSWMQTLWPLQIILSKS
FLMCRPERKSYRQFLTLCHQCAIRRP*VPSGHGS
SSQKPNSPYLNLVSPTMKSWSRDSGNCRLYKVL
CKSNKVAYTISAPL*KRCRRKRPLKLAGNINQN
LKKLRDAGRSSPPSWLSIVKS*RSK*INSEKFRI
TYKP*RNGWLKLMFF*RRNGLPLGIQKF*KSS*
NSADF*SVIFRQFSPV*TVSMKVGRR*RMKQSQ
SLLRDLRQNSKNLTLSGITCANRSMPERRP*REV
WRKL*ASRKIYQRCTNG*HKLKKSILREILNIK
LQMNYRKQLKR*RELKKRPNKKKRK*NSLLSL*
IVS*LKLHL*HKRP*KRNLKL*PPTTSGSALG*M
GNARLWKKFGHVGMSYCHTWRKQTSG*MK*NLN
LKPLKTFLAELRKSLRC*IHLKI*CDIQRITQI
RFAYWHRP*QMAESWMS*SMRNLRHLILVGGNYM
KRL*GGKSCLNRASSLPRRLKNPYT*SRSPSHS
LTSSWQLILQTRWTQLKCLRKPRKSNLI*QVMR
SV*KK*RNIIRGRRLPKESCLRLMLHRKNYKMSP
*SFDYSRNQPILSSVYKKVR*F*MK*RCTCLHW
KQRVWNRK*YSHS*IIV*TCIKV*VK*SLKWKW
**RLDVRLYRKSRRKIPKNLMKE*QL*NCIIMSW
EQR*QKESNSWRNA*NCPVRCERK*MS*QNGWQ
LQIWN*QRDQQLKECLVIWILKLPGERLLKKRL
RNRRCT*RVSQR*ERP*KQFWARRRRWWKINSVF
*IVTG*LSPPEQKSG*IFCWNTRNTWKLLTRMW
TTSQSGSFRLTHFWMNQRKRNPSKKKTCLSV*R
QN*MTYAQRWTLHVTKQQT*WQTAVTTAGN**SP
KSQSSTIDLQPFHTELRLERPPFL*RNWSSLTQ
IYKNCLNHWRLKFSRG*I*KRKTSIKI*MKTMR
VL*KNCCKEETTYNKESQMRESERK*R*NSSCYR
QNIMLSRI*GLKEEKRL*KFLISGISTRGRLMI
S*NAWMTLKKN*PAYLSPEMKGK*RKLIGNCRR
RKRS*MQCVGKLRACLRMGPQWQWSQLRSSSASA
GGKLRANLLSFEDSTLHKFTLSVKKR*W**LKT
CLWKFLMCLLLI*LKSLMSHKPY*KWNNFSMLL
TSVLRTLKISLSKRSL*RI*KIVYNKAQVGLTLF
IARRQQHCKVQRLWKG*SYRKLSPSLISNGKKL
TKCTRTDKGDLTDLLRNGGVFIMI*RYLISG*Q
KLNSFSERHKFLRIGNMLNTNGILRNSRMALGSG
KLLSEH*MQLGKK*FSNPQKQMPVFYRKNWEA*
ICGGRRSANSCQTEKRG*KNKRISCQNFKEI*M
NLFYGWRKQITLLVSHLNLEKSSN*KKSLSKSSY
WWKSCPCAREFSNN*MKLEDPCL*VLP*AQKSK
INLKISSSRQISSG*RFPELYLRNKEKLKLK*K
TLGSLKKSLKTLKSS*IICCCGYLLLGISWKFIT
NQTKKDHLTLRKLK*QFKLNNRMWKRFCLKGSI
CTRKNQPLSQ*RGS*KI*ALSGRR*TVYFKS*G
QSSLT*LLD*PLLEPLLLRLLLW*HNLWLLRKLP
SPN*KCHLP*CWRYLLWQISTGLGQNLPTGFLC
LIKL*NHRG*WWVTLRISTR*SSSRRQQCRIWN
RGVPSWKNSLPLPKI*KTRPAIKRLEQSLRIELK
EFRISGMKYKNTFRTGGNS*MKC*RIQHNGWKL
RKKLSRS*DRPEPSLSHGRRVPIQ*MQSKRKSQ
KPSSWPKTSASGRQM*MWQMTWP*NFSGIILQMI
PEKST**QRISMPLGEAFIKG*VSERLLWKKLI
DYCNSSPWTWKSFLPGLQKLKQLPMSYRMLPVR
KGS*KTPRE*KS**NNGKTSKVKLKLTQMFITTW
MKTAKKS*DPWKVPMMQSCYKDVWIT*TSSGVN
FGKSLSTLGPIWKPVLTSGSVCTFLCRNFWCGY
S*KMMN*AGRHLLEATFQQFRSRTMYIGPSRGN*
KLKNL*S*VLLRLYEYF*QSSLWKD*RNSTRSP
ESCLLRREPRMSLGFYESRLRRSILSGKN*TCT
PLTGREK*MRPLKDSGNFKRPRMSWTSSCAKLR*
SRDPGSPWAISSLTLSKITSRKSRHFEEKLRL*
KRT*ATSMTLLASLPLWAFSSHRITSALWKT*T
PDGSFCRWPSRTESGSCMKPTGTLVQHLSTFFPR
LSRVPGREPSRQTKCPTISTTRLKQLAGTIPK*
QSSTSL*LT*IMSDSQLIGLP*NSEDCRRPFAW
IS*ACQLHVMPWTSTTSSKMTSPWISCRLLIV*P
LFMTAWSKSTTIWSTSLSAWICV*TGC*MFMIR
DEQGGSVSCLLKLASFPCVKHIWKTSTDTFSSK
WQVQQDFVTSAGWASFCMILSKFQDSWVKLHPLG
AVTLSQVSGAASNLLIISQRSKRPSS*TG*DWN
PSPWCGCPSCTEWLLQKLPSIRPNVTSAKSVQS
LDSGTGV*STLIMTSAKAAFFLVELQKAIKCTIP
WWNIALRLHQEKMFETLPRY*KTNFEPKGILRS
IPEWATCQCRLS*RGTTWKLPLL*STSGQ*ILR
LPRPLSFHTMILIHALNIMLAG*QKWKTAMDLI*
MIASLLMRA*MMNIC*SSITAKV*TRTPP*ASL
VVLPRS*FP*RVRKEGS*RES*QILRKKTGICK
QNMTV*SSSTNIKACPHCRPLLK*CPPLPRVPGM
LSSLLRPSYCVNTKAAWKPGCKSWKTTINSWSH
SYTG*GSCWSNPRQRPK*MAQRCPLLLPLYRGP
TAVSLCCSEWLAVKLRTPWVRKIFSVLPRTQAQG
*RR*WSNSTTPSLVQEEEIPLESQ*ERTQCRKS
FPHGR*FGQSDGVLSISHDR*RRSRINVLQLLI
PAWFL*YSYNKED*TVRVYKK*IYIFVKGSGIIL
*ISVVSKSVIVLLTMAGFTRLCNCTKKL*ENYM
*NLDS*ITCHFFIWNAFWVV*KFITVIKKDCKL
KCAL*KKVVYKNP*KQNKHTHTHIHTHTQNFEAA
HCFASFWRDIHMKFMAFSFFAY*R*DFLYHHTK
*LLHTAHLRTVS*VGQA*VFISYIYMSISI*IL
*LYR*RDTNFYRLTFSIF*MFMSHPNRKKLLLVS
HPGLPAWSRMDFSRSRKPGGNYTTLKHCLQLQM
FLILNNFPLTTKVK*SIGFF*REHVNEYTGLII
SE*VIGWLVD*LID*YIQLPAASNATI*I**CFS
GNQSEGILTL*TSEGIF*LPSSSRTMIGLEGYG
FPAHPCEGVGHSLSEGLDDCS*YIKFSVITTKL
LCPLLTVKRNWVVWFLLLF*IYCPMWDEFLNATR
HNLK*INFGKRCKTVAPSHL*Y*QVSTQKPMNC
VSILCISLRVVPHRFVSK*ERRQIDSNVTKKPF
LVD*TG*IYKQTNKNCSKKRRKAQEEKLRTGRKK
LYSFMPFYFFLIFKSFIQ*IPPCDL*FCKSVTS
DIKCN*LLESGLTSSVISFWRVGFVHY**LIN*
HQTRLLMLFLPHFGFGVFLIIVHT*VHSFTTCPL
RYFLFPL*FFLFPS*FSKENPKL*GNKLPNYMK
IWFLSCIFFLYVTLDLFFTQGFLKLRFKTRGYF
TSY*EV*VSKFHSKIRGK*SA*IILF*SFCFSFR
HISSGVSLS*YLNKN*ELYCCILSIINLDIISC
CVLYNHQVLNCKS*CN*SINITWHVLSLFSGTE
FLLEYHNILCFNTNTVTFTNYFFKLQFYCIFTTY
QTSPNICLTIVL*YCFTVYLNKARSYV
Frame 1
CCL*S*ICENTSLFTATGVEETGAKRLCVCLLL*GWSEILCLLYLCLAMTLPPGLPYPIECVREKY
QQGNQQKAFL*RCVASLRRI*NV*GLIW*VKAAD
KCNSHSEE**IPQAFRSRLP*R*KRRCSKENIH
KMGKCTIF*VWEAAY*EPLQ*PTGWEAPPRPPR
RPDRAKTAKRKRIHKSSCPEQCQQGTAGFAEQ*C
*FSEYWKY*HRRWKS*TDSWFDLEYNPPLAGQK
CNEKYHGWIATNQQ*KDSPELGPTINS*LSTG*
CNQLHHQLV*WPGFECSHP*S*ARPI*LE*CGLP
AVSHTTTGTCIQHRQISIRHRETTRS*RC*YHL
SR*EVHLNVHHITLPSFASTSEH*SHPGSGNVA
KAT*SD*RRTFSVTSSNALFSTDHGQSSTGI*EN
FFP*ASIQELCLHTGCLCHHL*PYTEPISFTAF
GSS*RQVIWQFIDGE*SKPGPLSNSFRRSIIVA
SFC*GHIASTRRDF**CGSGERPVSYS*GVHDGF
DSPSGPGW*YSTIGK*ADWNRKIIRR*RN*STR
ADESPKFKMGMPQGS*HGKTKQFT*SFNGSPES
ETERVE*LANKNRRKNKENGGRASWT*S*RPKTP
STTT*GASRRSRTRTSQGQFSHSHGGGS**I*W
RSRNCCFGRTT*GIGRSMGKHL*MDRRPLGSFT
RHPSQMATSY*RTVPF*CMAFRKRRCSEQDSHNW
L*RSK*NVIKSSKTGRFKSGSRKEKAIHGQTVF
TQTRSSFNTEE*VSDPEDGSMAG*LCPVLG*FS
PKT*KEYSTDFTGCHHHSAITNTDNCNGNSNYGD
HKGTDPGKACSRGTSTTTSPKEEADYCGF*N*E
KVGC*YN*TSQLDYSLRSCVAES*ICNLSEGRQ
LLRLKRKSQCHRARKS*EVQKTARCQQISSGPGG
TDGE*GC*CR*HQTSLRTTEQPVDRILPVAK*E
T*LAGVSEQHHRFL*SATTIGADDNYC*KLVEN
PTHHPIRANSN*KSVKNL*G*SQPAIRSSTSN*T
IKNSKHSPERERTRTHVPGCRLCGLYKSF*ASL
F*CAGQRERATDNF*HFATNALSGDHECHQDMG
PAVRNQTLHTST*CHRL*NHGAETRGIAGFTKFS
ARATKWPILSQHHCERDVEESAL*N*PEISIRI
*RN*GTLEEALLPAG*ALSKARGANE*TPKNSE
SHTNPEEMDG*S*CFSEGGMACPWGFRNSKKAAE
TVQTFSQ*YSDNSAQSKQCQ*RWAEDKE*SRAR
VCFET*DRTQRT*HSVGSHVPTGLCQKGGLEGR
FGENCKPPERSIRDARMDDTS*RRVS*ERF*I*N
SR*ITESS*RDEES*RRGPTKRSESETPY*VCK
*CHSSSSTCSTRGLKKGT*NSNHQLPVALH*AE
WEMQDFGRSLGMLA*VIVILGESKQVAK*SRI*T
*NH*KHSWRS*GNL*GARFT*KFDATFRG*PKS
DSHIGTDPNRWRSHG*ANQ*GT*DI*FSLEGTT
*RGCKEAKVA*TEHPVCPGD*KILTLNPGVPHIH
*QAVGSLYCRQGGRSSNASGSPENPI*FDKS*D
QFRRNEET*SGEGGCPKSPVSD*CCTEKITRCL
HEVSIIPETSQF*AASTRK*DDFR*SEDALACIG
NKECGTGSSTVTAKSLCELV*KSE*SEV*SGNG
DKDWTSDCTEKADGKSQRT**KSNSFEIAL**A
GSKGNRKKATVGEMLEIVP*DAKGNECLDRMAGS
YRYGIDKEISS*RNA**FGF*SCLGKGYSKRD*
ETEGAPEEYHRGRRGLENSFGQEGDVGGR*TQS
SE**LDSCHLPSRRVVKSFVGIPETHGNF*PECG
PHHKVDHSG*HTFG*IREKETPAKRRRA*AFKG
RTE*HTPKGGLYT*PSSKLDGKPR*PLQEISRA
PNLRAQPSICSHFTQN*DWKGLHSFEGIGAV*LR
YTKIA*TTGG*NSAGGESERGRLQ*RYE*RQ*G
YCKRIVAKRRQLTTKNHR*EKARGNKDKTAAVT
DKT*CSQGFEVSKKKKGSRNFSSVVSVQEAG**S
PEMLG*H*KKISQPT*AQR*KENKGN*SGIAEE
ERGAECSA*AS*GLV*GWGRNGSGANSDPAQQA
LAGN*EQICSVSKTQLCTNSHCP*RNDDGDD*RH
AFGNFLCAFYLFD*NHSCLTSPIRSGTTSQCS*
PLC*GL*RSL*ARGVSEEYKR*STTKLRSD*HY
S*QEDSSIAKCNACGKGEATGSSLPA*FPMGKS*
QNVQGPTRAI*QIC*EMAAFSL*YKDI*SVANR
S*TVSQKDTNS*ELGTC*IQMVS*GTPGWHWAA
ANCCQNIECNWGRNNSAILKNRCQYSTGKIGKPE
SAVAGGLQTAVRQKKEARRTKEYLVRISKRFK*
ICFMVGGSR*HC*YPT*TWKRAATKRKA*ASQV
TGGRVAPAPGNSQTIK*NWRTRACKCSHKPRRAR
*T*K*AQADKSPVDKGFQSFT*ETRRN*SSNKR
PWAA*KKA*RP*RAVKSSAAVVISY*ESVGNL*
PTKPRRTI*R*GN*NSSSS*TTGCGRDFV*RAAF
VQGKTSHSASEEEVRRSEL*VEGGKPFTSRAEG
KAA*PSSWTDHYWSLSYSDCYSGDTTCGY*GNC
HLQTRNAIFLDVGGTCSGRFQPGLDRTYRLAFSA
*SSYKITEGDGG*P*GYQRDDHQAEGNNAGFGT
EASPVGRTHYRCPKFEKQDQQSRG*NNHYGSN*
KNSESVG*STRTPSEPEATVE*NVKGFNTMAGS*
GRS*AGLRTGQSQA*VMEGGSLYSRCNPKENHR
NQAVGQRPPPVADKCRCGK*LGPETSPGLFCR*
YQKSPHDNREYQCLLEKHS*KGE*ARGCFGRNS*
ITATVPPGPGKVSCLAYRS*NNCQCPTGCYP*G
KAPRRLQGSKRADETMARPPR*N*SSHRCLSQP
G*KQPKNPEIPGRFR*CSPVTKTFG*HELQVE*T
SEKVSQH*VPFGSQF*PVEASAPFSAGTSGVAT
AER**IKPAGTYWRRLSSSSEAERCT*GLQEGI
EN*RTCNHEYS*DCTNISDRAAFGRTRETLPGAQ
RAAS*GESPECHSASTKAG*GGQY*VGKIEPAL
R*LAEKNR*DP*KTPGTSRGHG*AGPQAAPS*G
DQGILAARGRSPH*LSPRSPRESQGTSRRNCASE
REREPRQ*PCSPAYHFGHSALTV*PQHSGRPEH
QMEASAGGRRGPSQAAA*SPQGLWSSISALSFH
VCPGSLGESHLAKQSALLYQPRDSNNLLGPSQND
RALPVFS*PE*CQILSL*DCHETPKTAEGPLLG
SLEPVSCM*CLGPAQPQAK*PAHGYPADY*LFD
HYL*PPGARAQQFGQRPSLRGYVSELAAECL*YG
TNREDPCPVF*NWHHFPV*STFGRQVQIPFQAS
GKFNRIL*PAQAGPPSA*FYPNSKTVG*SCILW
GQ*H*AKCPELLPIC***ARDRSGPLPRLDETGT
PVHGVAARPAQSGCCRNCQASGQM*HLQRVSNH
WIQVQESKAL*L*HLPKLLFFWSSCKRP*NALS
HGGILHSDYIRRRCSRLCQGTKKQISNQKVFCEA
SPNGLPASADCLRGGQHGNSRYSDQLLASRFCA
CLVPSAFTR*YSFTH*TLC*QASRNGKQQWILS
K**HLS**EHR**TFVNPALLPKFEPGLPPEPAS
*SCPDLDFLRE*GKRGARENPSRS*GRKQESAS
RI*PSKAAART*RPVPTAVPS*NDAHLSPESPG
C*AHC*GQATASTQRPPGSQDANPGRPQ*TAGVT
VTQAKAAAGATPGRGQSEWHNGVLSFYLSTEVR
QQSAYAAPSGWQSNFGLHG*GRSSQSSPGHKHR
VRGGDGATQQLLP*FKRKKYPWKANERGHNVGSL
FHMADDLGRAMESLVSVMTDEEGAE*MFYNS*F
PHGFYNIHTTKRIRQ*EFTRNKSIFL*RVVVLY
CRFQ*FLSLLLFC*QWQVLHVYAIVQKSYKKTTC
KILIAK*LAISLYGTHFGLFKNL*QL*RKIVN*
SVLYKKKLFIKTPKNKTNTHTHTYTHTHKTLRQ
RIVLHPFGVISI*NSWLFLFLHIKDKTSSTTTPN
DYYTLLI*ELSAEWGRLEFSFHISICL*VYKYY
SYIDKEIRISID*LFPFFKCSCHILIERNYF*S
VIQAYLLGLEWIFPGAGSQEETTPH*NIVYSSRC
FSF*TTFH*QRK*SKVLDFFKGNM*MNTQDLLY
QSE*SVGWLID*LIDTFSFLLLAMPRFRFNDAS
VEINQKVF*PCEHQKVFFNSQAVAGR**GWRAMD
SQPIPVKE*ATL*VKDWMIVHNT*SSL*LQLNY
YALFSQSKGTGWFGFCCFFRFIVPCGMSF*MPQ
DII*NK*TLGKGVKQ*PHHICDTDRYQPRSP*TV
FPSFAFLCE*FHTGL*VSKKEGKLIQMLQKNPS
WWIRQVKYINKQTKIAQKRGEKLKRKS*GLVGK
SFTLSCHFISF*FLNHSFNRYHRVTYNFANLLPL
TSSVISFWRVG*HQV*LAFGEWVLSIINN*LIN
IKHGFSCYFYLTLVLGCS**LCTPEFTASPLVH
CVIFFFLYNSFFFLHNFQKKTQSSKVTNYQIT*R
FGFCLAFFSFM*RWTFSLPKDF*NSDLKQGVTL
HPTKKFK*VSFILKSEVNRVHK*FCFNLFVFLL
DTLALE*VCHNI*TKIESFIAAF*A*LIWTLFRV
VFFITTKY*TVNHNVTEA*TSHGMFCHCFQVLS
SYLSIIIYCVLTPTL*HLRIIFLNFSFTAFSQH
IRLHQIYALLLYYSTALLCISIKHAVML
Frame 2
AVCEAESVRTPHYSRQPEWKKQVQKDCVFVCFCEAGQRFCACFICAWL*LYLQVYHTP*NV*EKST
NREISKKLSYEGV*PASAEFEMSEVSSGE*KLQ
INATAIQKNDKFHKHSEAGFPKDEREDVQKKTFT
KWVNAQFSKFGKQHIENLFSDLQDGRRLLDLLE
GLTGQKLPKEKGSTRVHALNNVNKALRVLQNNN
VDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKN
VMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVN
VINFTTSWSDGLALNALIHSHRPDLFDWNSVVC
QQSATQRLEHAFNIARYQLGIEKLLDPEDVDTTY
PDKKSILMYITSLFQVLPQQVSIEAIQEVEMLP
RPPKVTKEEHFQLHHQMHYSQQITVSLAQGYER
TSSPKPRFKSYAYTQAAYVTTSDPTRSPFPSQHL
EAPEDKSFGSSLMESEVNLDRYQTALEEVLSWL
LSAEDTLQAQGEISNDVEVVKDQFHTHEGYMMD
LTAHQGRVGNILQLGSKLIGTGKLSEDEETEVQE
QMNLLNSRWECLRVASMEKQSNLHRVLMDLQNQ
KLKELNDWLTKTEERTRKMEEEPLGPDLEDLKR
QVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSG
DHATAALEEQLKVLGDRWANICRWTEDRWVLLQ
DILLKWQRLTEEQCLFSAWLSEKEDAVNKIHTT
GFKDQNEMLSSLQKLAVLKADLEKKKQSMGKLYS
LKQDLLSTLKNKSVTQKTEAWLDNFARCWDNLV
QKLEKSTAQISQAVTTTQPSLTQTTVMETVTTV
TTREQILVKHAQEELPPPPPQKKRQITVDSEIRK
RLDVDITELHSWITRSEAVLQSPEFAIFRKEGN
FSDLKEKVNAIEREKAEKFRKLQDASRSAQALV
EQMVNEGVNADSIKQASEQLNSRWIEFCQLLSER
LNWLEYQNNIIAFYNQLQQLEQMTTTAENWLKI
QPTTPSEPTAIKSQLKICKDEVNRLSDLQPQIE
RLKIQSIALKEKGQGPMFLDADFVAFTNHFKQVF
SDVQAREKELQTIFDTLPPMRYQETMSAIRTWV
QQSETKLSIPQLSVTDYEIMEQRLGELQALQSS
LQEQQSGLYYLSTTVKEMSKKAPSEISRKYQSEF
EEIEGRWKKLSSQLVEHCQKLEEQMNKLRKIQN
HIQTLKKWMAEVDVFLKEEWPALGDSEILKKQL
KQCRLLVSDIQTIQPSLNSVNEGGQKIKNEAEPE
FASRLETELKELNTQWDHMCQQVYARKEALKGG
LEKTVSLQKDLSEMHEWMTQAEEEYLERDFEYK
TPDELQKAVEEMKRAKEEAQQKEAKVKLLTESVN
SVIAQAPPVAQEALKKELETLTTNYQWLCTRLN
GKCKTLEEVWACWHELLSYLEKANKWLNEVEFK
LKTTENIPGGAEEISEVLDSLENLMRHSEDNPNQ
IRILAQTLTDGGVMDELINEELETFNSRWRELH
EEAVRRQKLLEQSIQSAQETEKSLHLIQESLTF
IDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEI
SLEEMKKHNQGKEAAQRVLSQIDVAQKKLQDVS
MKFRLFQKPANFEQRLQESKMILDEVKMHLPAL
ETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMV
IKTGRQIVQKKQTENPKELDERVTALKLHYNEL
GAKVTERKQQLEKCLKLSRKMRKEMNVLTEWLA
ATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIE
KQKVHLKSITEVGEALKTVLGKKETLVEDKLSL
LNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNV
DHITKWIIQADTLLDESEKKKPQQKEDVLKRLKA
ELNDIRPKVDSTRDQAANLMANRGDHCRKLVEP
QISELNHRFAAISHRIKTGKASIPLKELEQFNS
DIQKLLEPLEAEIQQGVNLKEEDFNKDMNEDNEG
TVKELLQRGDNLQQRITDERKREEIKIKQQLLQ
TKHNALKDLRSQRRKKALEISHQWYQYKRQADD
LLKCLDDIEKKLASLPEPRDERKIKEIDRELQKK
KEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKR
WREIESKFAQFRRLNFAQIHTVREETMMVMTED
MPLEISYVPSTYLTEITHVSQALLEVEQLLNAPD
LCAKDFEDLFKQEESLKNIKDSLQQSSGRIDII
HSKKTAALQSATPVERVKLQEALSQLDFQWEKV
NKMYKDRQGRFDRSVEKWRRFHYDIKIFNQWLTE
AEQFLRKTQIPENWEHAKYKWYLKELQDGIGQR
QTVVRTLNATGEEIIQQSSKTDASILQEKLGSL
NLRWQEVCKQLSDRKKRLEEQKNILSEFQRDLNE
FVLWLEEADNIASIPLEPGKEQQLKEKLEQVKL
LVEELPLRQGILKQLNETGGPVLVSAPISPEEQ
DKLENKLKQTNLQWIKVSRALPEKQGEIEAQIKD
LGQLEKKLEDLEEQLNHLLLWLSPIRNQLEIYN
QPNQEGPFDVKETEIAVQAKQPDVEEILSKGQH
LYKEKPATQPVKRKLEDLSSEWKAVNRLLQELRA
KQPDLAPGLTTIGASPTQTVTLVTQPVVTKETA
ISKLEMPSSLMLEVPALADFNRAWTELTDWLSL
LDQVIKSQRVMVGDLEDINEMIIKQKATMQDLEQ
RRPQLEELITAAQNLKNKTSNQEARTIITDRIE
RIQNQWDEVQEHLQNRRQQLNEMLKDSTQWLEA
KEEAEQVLGQARAKLESWKEGPYTVDAIQKKITE
TKQLAKDLRQWQTNVDVANDLALKLLRDYSADD
TRKVHMITENINASWRSIHKRVSEREAALEETH
RLLQQFPLDLEKFLAWLTEAETTANVLQDATRKE
RLLEDSKGVKELMKQWQDLQGEIEAHTDVYHNL
DENSQKILRSLEGSDDAVLLQRRLDNMNFKWSE
LRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQ
LKDDELSRQAPIGGDFPAVQKQNDVHRAFKREL
KTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEP
RELPPEERAQNVTRLLRKQAEEVNTEWEKLNLHS
ADWQRKIDETLERLRELQEATDELDLKLRQAEV
IKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPL
KENVSHVNDLARQLTTLGIQLSPYNLSTLEDLNT
RWKLLQVAVEDRVRQLHEAHRDFGPASQHFLST
SVQGPWERAISPNKVPYYINHETQTTCWDHPKM
TELYQSLADLNNVRFSAYRTAMKLRRLQKALCLD
LLSLSAACDALDQHNLKQNDQPMDILQIINCLT
TIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDT
GRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQV
ASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFG
GSNIEPSVRSCFQFANNKPEIEAALFLDWMRLE
PQSMVWLPVLHRVAAAETAKHQAKCNICKECPII
GFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYP
MVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAK
HPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAP
ASSPQLSHDDTHSRIEHYASRLAEMENSNGSYL
NDSISPNESIDDEHLLIQHYCQSLNQDSPLSQP
RSPAQILISLESEERGELERILADLEEENRNLQA
EYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRD
AELIAEAKLLRQHKGRLEARMQILEDHNKQLES
QLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSD
SSQPMLLRVVGSQTSDSMGEEDLLSPPQDTSTG
LEEVMEQLNNSFPSSRGRNTPGKPMREDTM*EV
FSTWQMIWAERWSP*YQS*QMKKEQNKCFTTPDS
RMVFIIFIQQRGLDSKSLQEINLYFCEG*WYYT
VDFSSF*VCYCFVNNGRFYTSMQLYKKVIRKLH
VKS**LNNLPFLYMERILGCLKIYNSYKERL*TK
VCFIKKSCL*KPLKTKQTHTHTHTHTHTKL*GS
ALFCILLA*YPYEIHGFFFFCILKIRLPLPPHQ
MTTTHCSFENCQLSGAGLSFHFIYLYVYKYINTI
VI*IKRYEFL*TDFFHFLNVHVTS**KEITSSQ
SSRLTCLV*NGFFPEPEARRKLHHTKTLSTAPD
VSHFKQLSTDNESKVKYWIFLKGTCE*IHRTYYI
RVSNRLVG*LID*LIHSASCC*QCHDLDLMMLQ
WKSIRRYSDLVNIRRYFLTPKQ*QDDDRAGGLW
IPSPSL*RSRPLFK*RIG*LFIIHKVLCNYN*II
MPSSHSQKELGGLVFVAFLDLLSHVG*VFKCHK
T*FKINKLWEKV*NSSPITFVILTGINPEAHEL
CFHPLHFSASSSTQVCK*VRKKAN*FKCYKKTLL
GGLDRLNI*TNKQKLLKKEEKSSRGKAKDW*EK
ALLFHAILFLFDF*IIHSIDTTV*PIILQICYL
*HQV*LAFGEWADIKCN*LLESGFCPLLIIN*LT
SNTASHAISTSLWFWGVPDNCAHLSSQLHHLSI
ALFSFSFIILSFSFIIFKRKPKALR*QITKLHE
DLVFVLHFFPLCDAGPFLYPRIFKTQI*NKGLLY
ILLRSLSK*VSF*NQR*IECINNFVLIFLFFF*
TH*LWSESVIIFEQKLRALLLHFKHN*FGHYFV
LCSL*PPSIKL*IIM*LKHKHHMACFVIVFRY*V
LT*VS*YIVF*HQHCNIYELFF*TSVLLHFHNI
SDFTKYMPYYCIIVLLYCVSQ*STQLCY
Sequence NM_000109
CGTTAAATGCAAACGCTGCTCTGGCTCATGTGTTTGCTCCGAGGTATAGGTTTTGTTCGACTGACGTATCAGATAGTCAGAGTGGTTACCACACCGACGTTGTAGCAGCTGCATAATAAATGACTGAAAGAATCATGTTAGGCATGCCCACCTAACCTAACTTGAATCATGCGAAAGGGGAGCTGTTGGAATTCAAATAG
ACTTTCTGGTTCCCAGCAGTCGGCAGTAATAGAATGCTTTCAGGAAGATGACAGAATCAGGAGAAAGATGCTGTTTTGCACTATCTTGATTTGTTACAGC
AGCCAACTTATTGGCATGATGGAGTGACAGGAAAAACAGCTGGCATGGAAGATGAAAGAGAAGATGTTCAAAAGAAAACATTCACAAAATGGGTAAATGC
ACAATTTTCTAAGTTTGGGAAGCAGCATATTGAGAACCTCTTCAGTGACCTACAGGATGGGAGGCGCCTCCTAGACCTCCTCGAAGGCCTGACAGGGCAA
AAACTGCCAAAAGAAAAAGGATCCACAAGAGTTCATGCCCTGAACAATGTCAACAAGGCACTGCGGGTTTTGCAGAACAATAATGTTGATTTAGTGAATA
TTGGAAGTACTGACATCGTAGATGGAAATCATAAACTGACTCTTGGTTTGATTTGGAATATAATCCTCCACTGGCAGGTCAAAAATGTAATGAAAAATAT
CATGGCTGGATTGCAACAAACCAACAGTGAAAAGATTCTCCTGAGCTGGGTCCGACAATCAACTCGTAATTATCCACAGGTTAATGTAATCAACTTCACC
ACCAGCTGGTCTGATGGCCTGGCTTTGAATGCTCTCATCCATAGTCATAGGCCAGACCTATTTGACTGGAATAGTGTGGTTTGCCAGCAGTCAGCCACAC
AACGACTGGAACATGCATTCAACATCGCCAGATATCAATTAGGCATAGAGAAACTACTCGATCCTGAAGATGTTGATACCACCTATCCAGATAAGAAGTC
CATCTTAATGTACATCACATCACTCTTCCAAGTTTTGCCTCAACAAGTGAGCATTGAAGCCATCCAGGAAGTGGAAATGTTGCCAAGGCCACCTAAAGTG
ACTAAAGAAGAACATTTTCAGTTACATCATCAAATGCACTATTCTCAACAGATCACGGTCAGTCTAGCACAGGGATATGAGAGAACTTCTTCCCCTAAGC
CTCGATTCAAGAGCTATGCCTACACACAGGCTGCTTATGTCACCACCTCTGACCCTACACGGAGCCCATTTCCTTCACAGCATTTGGAAGCTCCTGAAGA
CAAGTCATTTGGCAGTTCATTGATGGAGAGTGAAGTAAACCTGGACCGTTATCAAACAGCTTTAGAAGAAGTATTATCGTGGCTTCTTTCTGCTGAGGAC
ACATTGCAAGCACAAGGAGAGATTTCTAATGATGTGGAAGTGGTGAAAGACCAGTTTCATACTCATGAGGGGTACATGATGGATTTGACAGCCCATCAGG
GCCGGGTTGGTAATATTCTACAATTGGGAAGTAAGCTGATTGGAACAGGAAAATTATCAGAAGATGAAGAAACTGAAGTACAAGAGCAGATGAATCTCCT
AAATTCAAGATGGGAATGCCTCAGGGTAGCTAGCATGGAAAAACAAAGCAATTTACATAGAGTTTTAATGGATCTCCAGAATCAGAAACTGAAAGAGTTG
AATGACTGGCTAACAAAAACAGAAGAAAGAACAAGGAAAATGGAGGAAGAGCCTCTTGGACCTGATCTTGAAGACCTAAAACGCCAAGTACAACAACATA
AGGTGCTTCAAGAAGATCTAGAACAAGAACAAGTCAGGGTCAATTCTCTCACTCACATGGTGGTGGTAGTTGATGAATCTAGTGGAGATCACGCAACTGC
TGCTTTGGAAGAACAACTTAAGGTATTGGGAGATCGATGGGCAAACATCTGTAGATGGACAGAAGACCGCTGGGTTCTTTTACAAGACATCCTTCTCAAA
TGGCAACGTCTTACTGAAGAACAGTGCCTTTTTAGTGCATGGCTTTCAGAAAAAGAAGATGCAGTGAACAAGATTCACACAACTGGCTTTAAAGATCAAA
ATGAAATGTTATCAAGTCTTCAAAAACTGGCCGTTTTAAAAGCGGATCTAGAAAAGAAAAAGCAATCCATGGGCAAACTGTATTCACTCAAACAAGATCT
TCTTTCAACACTGAAGAATAAGTCAGTGACCCAGAAGACGGAAGCATGGCTGGATAACTTTGCCCGGTGTTGGGATAATTTAGTCCAAAAACTTGAAAAG
AGTACAGCACAGATTTCACAGGCTGTCACCACCACTCAGCCATCACTAACACAGACAACTGTAATGGAAACAGTAACTACGGTGACCACAAGGGAACAGA
TCCTGGTAAAGCATGCTCAAGAGGAACTTCCACCACCACCTCCCCAAAAGAAGAGGCAGATTACTGTGGATTCTGAAATTAGGAAAAGGTTGGATGTTGA
TATAACTGAACTTCACAGCTGGATTACTCGCTCAGAAGCTGTGTTGCAGAGTCCTGAATTTGCAATCTTTCGGAAGGAAGGCAACTTCTCAGACTTAAAA
GAAAAAGTCAATGCCATAGAGCGAGAAAAAGCTGAGAAGTTCAGAAAACTGCAAGATGCCAGCAGATCAGCTCAGGCCCTGGTGGAACAGATGGTGAATG
AGGGTGTTAATGCAGATAGCATCAAACAAGCCTCAGAACAACTGAACAGCCGGTGGATCGAATTCTGCCAGTTGCTAAGTGAGAGACTTAACTGGCTGGA
GTATCAGAACAACATCATCGCTTTCTATAATCAGCTACAACAATTGGAGCAGATGACAACTACTGCTGAAAACTGGTTGAAAATCCAACCCACCACCCCA
TCAGAGCCAACAGCAATTAAAAGTCAGTTAAAAATTTGTAAGGATGAAGTCAACCGGCTATCAGATCTTCAACCTCAAATTGAACGATTAAAAATTCAAA
GCATAGCCCTGAAAGAGAAAGGACAAGGACCCATGTTCCTGGATGCAGACTTTGTGGCCTTTACAAATCATTTTAAGCAAGTCTTTTCTGATGTGCAGGC
CAGAGAGAAAGAGCTACAGACAATTTTTGACACTTTGCCACCAATGCGCTATCAGGAGACCATGAGTGCCATCAGGACATGGGTCCAGCAGTCAGAAACC
AAACTCTCCATACCTCAACTTAGTGTCACCGACTATGAAATCATGGAGCAGAGACTCGGGGAATTGCAGGCTTTACAAAGTTCTCTGCAAGAGCAACAAA
GTGGCCTATACTATCTCAGCACCACTGTGAAAGAGATGTCGAAGAAAGCGCCCTCTGAAATTAGCCGGAAATATCAATCAGAATTTGAAGAAATTGAGGG
ACGCTGGAAGAAGCTCTCCTCCCAGCTGGTTGAGCATTGTCAAAAGCTAGAGGAGCAAATGAATAAACTCCGAAAAATTCAGAATCACATACAAACCCTG
AAGAAATGGATGGCTGAAGTTGATGTTTTTCTGAAGGAGGAATGGCCTGCCCTTGGGGATTCAGAAATTCTAAAAAAGCAGCTGAAACAGTGCAGACTTT
TAGTCAGTGATATTCAGACAATTCAGCCCAGTCTAAACAGTGTCAATGAAGGTGGGCAGAAGATAAAGAATGAAGCAGAGCCAGAGTTTGCTTCGAGACT
TGAGACAGAACTCAAAGAACTTAACACTCAGTGGGATCACATGTGCCAACAGGTCTATGCCAGAAAGGAGGCCTTGAAGGGAGGTTTGGAGAAAACTGTA
AGCCTCCAGAAAGATCTATCAGAGATGCACGAATGGATGACACAAGCTGAAGAAGAGTATCTTGAGAGAGATTTTGAATATAAAACTCCAGATGAATTAC
AGAAAGCAGTTGAAGAGATGAAGAGAGCTAAAGAAGAGGCCCAACAAAAAGAAGCGAAAGTGAAACTCCTTACTGAGTCTGTAAATAGTGTCATAGCTCA
AGCTCCACCTGTAGCACAAGAGGCCTTAAAAAAGGAACTTGAAACTCTAACCACCAACTACCAGTGGCTCTGCACTAGGCTGAATGGGAAATGCAAGACT
TTGGAAGAAGTTTGGGCATGTTGGCATGAGTTATTGTCATACTTGGAGAAAGCAAACAAGTGGCTAAATGAAGTAGAATTTAAACTTAAAACCACTGAAA
ACATTCCTGGCGGAGCTGAGGAAATCTCTGAGGTGCTAGATTCACTTGAAAATTTGATGCGACATTCAGAGGATAACCCAAATCAGATTCGCATATTGGC
ACAGACCCTAACAGATGGCGGAGTCATGGATGAGCTAATCAATGAGGAACTTGAGACATTTAATTCTCGTTGGAGGGAACTACATGAAGAGGCTGTAAGG
AGGCAAAAGTTGCTTGAACAGAGCATCCAGTCTGCCCAGGAGACTGAAAAATCCTTACACTTAATCCAGGAGTCCCTCACATTCATTGACAAGCAGTTGG
CAGCTTATATTGCAGACAAGGTGGACGCAGCTCAAATGCCTCAGGAAGCCCAGAAAATCCAATCTGATTTGACAAGTCATGAGATCAGTTTAGAAGAAAT
GAAGAAACATAATCAGGGGAAGGAGGCTGCCCAAAGAGTCCTGTCTCAGATTGATGTTGCACAGAAAAAATTACAAGATGTCTCCATGAAGTTTCGATTA
TTCCAGAAACCAGCCAATTTTGAGCAGCGTCTACAAGAAAGTAAGATGATTTTAGATGAAGTGAAGATGCACTTGCCTGCATTGGAAACAAAGAGTGTGG
AACAGGAAGTAGTACAGTCACAGCTAAATCATTGTGTGAACTTGTATAAAAGTCTGAGTGAAGTGAAGTCTGAAGTGGAAATGGTGATAAAGACTGGACG
TCAGATTGTACAGAAAAAGCAGACGGAAAATCCCAAAGAACTTGATGAAAGAGTAACAGCTTTGAAATTGCATTATAATGAGCTGGGAGCAAAGGTAACA
GAAAGAAAGCAACAGTTGGAGAAATGCTTGAAATTGTCCCGTAAGATGCGAAAGGAAATGAATGTCTTGACAGAATGGCTGGCAGCTACAGATATGGAAT
TGACAAAGAGATCAGCAGTTGAAGGAATGCCTAGTAATTTGGATTCTGAAGTTGCCTGGGGAAAGGCTACTCAAAAAGAGATTGAGAAACAGAAGGTGCA
CCTGAAGAGTATCACAGAGGTAGGAGAGGCCTTGAAAACAGTTTTGGGCAAGAAGGAGACGTTGGTGGAAGATAAACTCAGTCTTCTGAATAGTAACTGG
ATAGCTGTCACCTCCCGAGCAGAAGAGTGGTTAAATCTTTTGTTGGAATACCAGAAACACATGGAAACTTTTGACCAGAATGTGGACCACATCACAAAGT
GGATCATTCAGGCTGACACACTTTTGGATGAATCAGAGAAAAAGAAACCCCAGCAAAAAGAAGACGTGCTTAAGCGTTTAAAGGCAGAACTGAATGACAT
ACGCCCAAAGGTGGACTCTACACGTGACCAAGCAGCAAACTTGATGGCAAACCGCGGTGACCACTGCAGGAAATTAGTAGAGCCCCAAATCTCAGAGCTC
AACCATCGATTTGCAGCCATTTCACACAGAATTAAGACTGGAAAGGCCTCCATTCCTTTGAAGGAATTGGAGCAGTTTAACTCAGATATACAAAAATTGC
TTGAACCACTGGAGGCTGAAATTCAGCAGGGGGTGAATCTGAAAGAGGAAGACTTCAATAAAGATATGAATGAAGACAATGAGGGTACTGTAAAAGAATT
GTTGCAAAGAGGAGACAACTTACAACAAAGAATCACAGATGAGAGAAAGCGAGAGGAAATAAAGATAAAACAGCAGCTGTTACAGACAAAACATAATGCT
CTCAAGGATTTGAGGTCTCAAAGAAGAAAAAAGGCTCTAGAAATTTCTCATCAGTGGTATCAGTACAAGAGGCAGGCTGATGATCTCCTGAAATGCTTGG
ATGACATTGAAAAAAAATTAGCCAGCCTACCTGAGCCCAGAGATGAAAGGAAAATAAAGGAAATTGATCGGGAATTGCAGAAGAAGAAAGAGGAGCTGAA
TGCAGTGCGTAGGCAAGCTGAGGGCTTGTCTGAGGATGGGGCCGCAATGGCAGTGGAGCCAACTCAGATCCAGCTCAGCAAGCGCTGGCGGGAAATTGAG
AGCAAATTTGCTCAGTTTCGAAGACTCAACTTTGCACAAATTCACACTGTCCGTGAAGAAACGATGATGGTGATGACTGAAGACATGCCTTTGGAAATTT
CTTATGTGCCTTCTACTTATTTGACTGAAATCACTCATGTCTCACAAGCCCTATTAGAAGTGGAACAACTTCTCAATGCTCCTGACCTCTGTGCTAAGGA
CTTTGAAGATCTCTTTAAGCAAGAGGAGTCTCTGAAGAATATAAAAGATAGTCTACAACAAAGCTCAGGTCGGATTGACATTATTCATAGCAAGAAGACA
GCAGCATTGCAAAGTGCAACGCCTGTGGAAAGGGTGAAGCTACAGGAAGCTCTCTCCCAGCTTGATTTCCAATGGGAAAAAGTTAACAAAATGTACAAGG
ACCGACAAGGGCGATTTGACAGATCTGTTGAGAAATGGCGGCGTTTTCATTATGATATAAAGATATTTAATCAGTGGCTAACAGAAGCTGAACAGTTTCT
CAGAAAGACACAAATTCCTGAGAATTGGGAACATGCTAAATACAAATGGTATCTTAAGGAACTCCAGGATGGCATTGGGCAGCGGCAAACTGTTGTCAGA
ACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGATGCCAGTATTCTACAGGAAAAATTGGGAAGCCTGAATCTGCGGTGGCAGG
AGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGGCTAGAAGAACAAAAGAATATCTTGTCAGAATTTCAAAGAGATTTAAATGAATTTGTTTTATGGTT
GGAGGAAGCAGATAACATTGCTAGTATCCCACTTGAACCTGGAAAAGAGCAGCAACTAAAAGAAAAGCTTGAGCAAGTCAAGTTACTGGTGGAAGAGTTG
CCCCTGCGCCAGGGAATTCTCAAACAATTAAATGAAACTGGAGGACCCGTGCTTGTAAGTGCTCCCATAAGCCCAGAAGAGCAAGATAAACTTGAAAATA
AGCTCAAGCAGACAAATCTCCAGTGGATAAAGGTTTCCAGAGCTTTACCTGAGAAACAAGGAGAAATTGAAGCTCAAATAAAAGACCTTGGGCAGCTTGA
AAAAAAGCTTGAAGACCTTGAAGAGCAGTTAAATCATCTGCTGCTGTGGTTATCTCCTATTAGGAATCAGTTGGAAATTTATAACCAACCAAACCAAGAA
GGACCATTTGACGTTAAGGAAACTGAAATAGCAGTTCAAGCTAAACAACCGGATGTGGAAGAGATTTTGTCTAAAGGGCAGCATTTGTACAAGGAAAAAC
CAGCCACTCAGCCAGTGAAGAGGAAGTTAGAAGATCTGAGCTCTGAGTGGAAGGCGGTAAACCGTTTACTTCAAGAGCTGAGGGCAAAGCAGCCTGACCT
AGCTCCTGGACTGACCACTATTGGAGCCTCTCCTACTCAGACTGTTACTCTGGTGACACAACCTGTGGTTACTAAGGAAACTGCCATCTCCAAACTAGAA
ATGCCATCTTCCTTGATGTTGGAGGTACCTGCTCTGGCAGATTTCAACCGGGCTTGGACAGAACTTACCGACTGGCTTTCTCTGCTTGATCAAGTTATAA
AATCACAGAGGGTGATGGTGGGTGACCTTGAGGATATCAACGAGATGATCATCAAGCAGAAGGCAACAATGCAGGATTTGGAACAGAGGCGTCCCCAGTT
GGAAGAACTCATTACCGCTGCCCAAAATTTGAAAAACAAGACCAGCAATCAAGAGGCTAGAACAATCATTACGGATCGAATTGAAAGAATTCAGAATCAG
TGGGATGAAGTACAAGAACACCTTCAGAACCGGAGGCAACAGTTGAATGAAATGTTAAAGGATTCAACACAATGGCTGGAAGCTAAGGAAGAAGCTGAGC
AGGTCTTAGGACAGGCCAGAGCCAAGCTTGAGTCATGGAAGGAGGGTCCCTATACAGTAGATGCAATCCAAAAGAAAATCACAGAAACCAAGCAGTTGGC
CAAAGACCTCCGCCAGTGGCAGACAAATGTAGATGTGGCAAATGACTTGGCCCTGAAACTTCTCCGGGATTATTCTGCAGATGATACCAGAAAAGTCCAC
ATGATAACAGAGAATATCAATGCCTCTTGGAGAAGCATTCATAAAAGGGTGAGTGAGCGAGAGGCTGCTTTGGAAGAAACTCATAGATTACTGCAACAGT
TCCCCCTGGACCTGGAAAAGTTTCTTGCCTGGCTTACAGAAGCTGAAACAACTGCCAATGTCCTACAGGATGCTACCCGTAAGGAAAGGCTCCTAGAAGA
CTCCAAGGGAGTAAAAGAGCTGATGAAACAATGGCAAGACCTCCAAGGTGAAATTGAAGCTCACACAGATGTTTATCACAACCTGGATGAAAACAGCCAA
AAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTACAAAGACGTTTGGATAACATGAACTTCAAGTGGAGTGAACTTCGGAAAAAGTCTC
TCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCGTCTGCACCTTTCTCTGCAGGAACTTCTGGTGTGGCTACAGCTGAAAGATGATGA
ATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAGAAGCAGAACGATGTACATAGGGCCTTCAAGAGGGAATTGAAAACTAAAGAACCT
GTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGCAGCCTTTGGAAGGACTAGAGAAACTCTACCAGGAGCCCAGAGAGCTGCCTCCTG
AGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGAGGAGGTCAATACTGAGTGGGAAAAATTGAACCTGCACTCCGCTGACTGGCAGAG
AAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCCACGGATGAGCTGGACCTCAAGCTGCGCCAAGCTGAGGTGATCAAGGGATCCTGG
CAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCGAGAAAGTCAAGGCACTTCGAGGAGAAATTGCGCCTCTGAAAGAGAACGTGAGCC
ACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCTCTCACCGTATAACCTCAGCACTCTGGAAGACCTGAACACCAGATGGAAGCTTCT
GCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCACAGGGACTTTGGTCCAGCATCTCAGCACTTTCTTTCCACGTCTGTCCAGGGTCCC
TGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACCACGAGACTCAAACAACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGT
CTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGCCATGAAACTCCGAAGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTC
AGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGACCAGCCCATGGATATCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGC
CTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGGATATGTGTCTGAACTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGA
TCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGCACATTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGG
ATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATCCAAATTCCAAGACAGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAG
CCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGATCGAAGCGGCCCTCTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGT
GGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCATCAGGCCAAATGTAACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAG
GAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGTCGAGTTGCAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGC
ACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTAAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCT
ACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCCCGTTACTCTGATCAACTTCTGGCCAGTAGATTCTGCGCCTGCCTCGTCCCCTCA
GCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATCTAAATGATAGCATCTCT
CCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTTTGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGA
TCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAAACAGGAATCTGCAAGCAGAATATGACCGTCT
AAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATGATGCCCACCTCTCCCCAGAGTCCCCGGGATGCTGAGCTCATTGCT
GAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCCTGGAAGACCACAATAAACAGCTGGAGTCACAGTTACACAGGCTAA
GGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTCCTCTCCTTCTACCTCTCTACAGAGGTCCGACAGCAGTCAGCCTAT
GCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTTCTCAGTCCTCCCCAGGACACAAGCACAGGGTTAGAGGAGGTGATG
GAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCCCTGGAAAGCCAATGAGAGAGGACACAATGTAGGAAGTCTTTTCCACATGGCAGA
TGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGAGCAGAATAAATGTTTTACAACTCCTGATTCCCGCATGGTTTTTAT
AATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTATATTTTTGTGAAGGGTAGTGGTATTATACTGTAGATTTCAGTAGT
TTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTTATAAGAAAACTACATGTAAAATCTTGATAGC
TAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAGAAAGATTGTAAACTAAAGTGTGCTTTATAAA
AAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACATACACACACACACACAAAACTTTGAGGCAGCGCATTGTTTTGCATC
CTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAAAGATAAGACTTCCTCTACCACCACACCAAATGACTACTACACACT
GCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATAAGTATATAAATACTATAGTTATATAGATAAA
GAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGAAATTACTTCTAGTCAGTCATCCAGGCTTACC
TGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACCACACTAAAACATTGTCTACAGCTCCAGATGTTTCTCATTTTAAAC
AACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAACATGTGAATGAATACACAGGACTTATTATATCAGAGTGAGTAATCG
GTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGATTTAATGATGCTTCAGTGGAAATCAATCAGA
AGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGGGCTGGAGGGCTATGGATTCCCAGCCCATCCC
TGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACATAAAGTTCTCTGTAATTACAACTAAATTATTATGCCCTCTTCTCA
CAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCCCATGTGGGATGAGTTTTTAAATGCCACAAGACATAATTTAAAATA
AATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGGTATCAACCCAGAAGCCCATGAACTGTGTTTCCATCCTTTGC
ATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTTACAAAAAAACCCTTCTTGGTGGATTAGACAG
GTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCAAGAGGAAAAGCTAAGGACTGGTAGGAAAAAGCTTTACTCTTTCAT
GCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACCGTGTGACCTATAATTTTGCAAATCTGTTACCTCTGACATCAAGTGTAAT
TAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAATAATTAATTAATTAACATCAAACACGGCTTC
TCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTTTT
TCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTCTAAGGTAACAAATTACCAAATTACATGAAGATTTGGTTTTTGTCT
TGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAAAACTCAGATTTAAAACAAGGGGTTACTTTACATCCTACTAAGAAG
TTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAGACACATTAGCTCTGG
AGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTTGGACATTATTTCGTGTTGTGTTCTTTATAAC
CACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGCATGTTTTGTCATTGTTTTCAGGTACTGAGTTCTTACTTGAGTATC
ATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTTTTAAACTTCAGTTTTACTGCATTTTCACAACATATCAGACTTCACCAAA
TATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
R*MQTLLWLMCLLRGIGFVRLTYQIVRVVTTPTL*QLHNK*LKESC*ACPPNLT*IMRKGSCWNSNR
LSGSQQSAVIECFQEDDRIRRKMLFCTILICYS
SQLIGMME*QEKQLAWKMKEKMFKRKHSQNG*M
HNFLSLGSSILRTSSVTYRMGGAS*TSSKA*QGK
NCQKKKDPQEFMP*TMSTRHCGFCRTIMLI**I
LEVLTS*MEIIN*LLV*FGI*SSTGRSKM**KI
SWLDCNKPTVKRFS*AGSDNQLVIIHRLM*STSP
PAGLMAWL*MLSSIVIGQTYLTGIVWFASSQPH
NDWNMHSTSPDIN*A*RNYSILKMLIPPIQIRS
PS*CTSHHSSKFCLNK*ALKPSRKWKCCQGHLK*
LKKNIFSYIIKCTILNRSRSV*HRDMRELLPLS
LDSRAMPTHRLLMSPPLTLHGAHFLHSIWKLLK
TSHLAVH*WRVK*TWTVIKQL*KKYYRGFFLLRT
HCKHKERFLMMWKW*KTSFILMRGT*WI*QPIR
AGLVIFYNWEVS*LEQENYQKMKKLKYKSR*IS
*IQDGNASG*LAWKNKAIYIEF*WISRIRN*KS*
MTG*QKQKKEQGKWRKSLLDLILKT*NAKYNNI
RCFKKI*NKNKSGSILSLTWWW*LMNLVEITQL
LLWKNNLRYWEIDGQTSVDGQKTAGFFYKTSFSN
GNVLLKNSAFLVHGFQKKKMQ*TRFTQLALKIK
MKCYQVFKNWPF*KRI*KRKSNPWANCIHSNKI
FFQH*RISQ*PRRRKHGWITLPGVGII*SKNLKR
VQHRFHRLSPPLSHH*HRQL*WKQ*LR*PQGNR
SW*SMLKRNFHHHLPKRRGRLLWILKLGKGWML
I*LNFTAGLLAQKLCCRVLNLQSFGRKATSQT*K
KKSMP*SEKKLRSSENCKMPADQLRPWWNRW*M
RVLMQIASNKPQNN*TAGGSNSASC*VRDLTGW
SIRTTSSLSIISYNNWSR*QLLLKTG*KSNPPPH
QSQQQLKVS*KFVRMKSTGYQIFNLKLND*KFK
A*P*KRKDKDPCSWMQTLWPLQIILSKSFLMCR
PERKSYRQFLTLCHQCAIRRP*VPSGHGSSSQKP
NSPYLNLVSPTMKSWSRDSGNCRLYKVLCKSNK
VAYTISAPL*KRCRRKRPLKLAGNINQNLKKLR
DAGRSSPPSWLSIVKS*RSK*INSEKFRITYKP*
RNGWLKLMFF*RRNGLPLGIQKF*KSS*NSADF
*SVIFRQFSPV*TVSMKVGRR*RMKQSQSLLRD
LRQNSKNLTLSGITCANRSMPERRP*REVWRKL*
ASRKIYQRCTNG*HKLKKSILREILNIKLQMNY
RKQLKR*RELKKRPNKKKRK*NSLLSL*IVS*L
KLHL*HKRP*KRNLKL*PPTTSGSALG*MGNARL
WKKFGHVGMSYCHTWRKQTSG*MK*NLNLKPLK
TFLAELRKSLRC*IHLKI*CDIQRITQIRFAYW
HRP*QMAESWMS*SMRNLRHLILVGGNYMKRL*G
GKSCLNRASSLPRRLKNPYT*SRSPSHSLTSSW
QLILQTRWTQLKCLRKPRKSNLI*QVMRSV*KK
*RNIIRGRRLPKESCLRLMLHRKNYKMSP*SFDY
SRNQPILSSVYKKVR*F*MK*RCTCLHWKQRVW
NRK*YSHS*IIV*TCIKV*VK*SLKWKW**RLD
VRLYRKSRRKIPKNLMKE*QL*NCIIMSWEQR*Q
KESNSWRNA*NCPVRCERK*MS*QNGWQLQIWN
*QRDQQLKECLVIWILKLPGERLLKKRLRNRRC
T*RVSQR*ERP*KQFWARRRRWWKINSVF*IVTG
*LSPPEQKSG*IFCWNTRNTWKLLTRMWTTSQS
GSFRLTHFWMNQRKRNPSKKKTCLSV*RQN*MT
YAQRWTLHVTKQQT*WQTAVTTAGN**SPKSQSS
TIDLQPFHTELRLERPPFL*RNWSSLTQIYKNC
LNHWRLKFSRG*I*KRKTSIKI*MKTMRVL*KN
CCKEETTYNKESQMRESERK*R*NSSCYRQNIML
SRI*GLKEEKRL*KFLISGISTRGRLMIS*NAW
MTLKKN*PAYLSPEMKGK*RKLIGNCRRRKRS*
MQCVGKLRACLRMGPQWQWSQLRSSSASAGGKLR
ANLLSFEDSTLHKFTLSVKKR*W**LKTCLWKF
LMCLLLI*LKSLMSHKPY*KWNNFSMLLTSVLR
TLKISLSKRSL*RI*KIVYNKAQVGLTLFIARRQ
QHCKVQRLWKG*SYRKLSPSLISNGKKLTKCTR
TDKGDLTDLLRNGGVFIMI*RYLISG*QKLNSF
SERHKFLRIGNMLNTNGILRNSRMALGSGKLLSE
H*MQLGKK*FSNPQKQMPVFYRKNWEA*ICGGR
RSANSCQTEKRG*KNKRISCQNFKEI*MNLFYG
WRKQITLLVSHLNLEKSSN*KKSLSKSSYWWKSC
PCAREFSNN*MKLEDPCL*VLP*AQKSKINLKI
SSSRQISSG*RFPELYLRNKEKLKLK*KTLGSL
KKSLKTLKSS*IICCCGYLLLGISWKFITNQTKK
DHLTLRKLK*QFKLNNRMWKRFCLKGSICTRKN
QPLSQ*RGS*KI*ALSGRR*TVYFKS*GQSSLT
*LLD*PLLEPLLLRLLLW*HNLWLLRKLPSPN*K
CHLP*CWRYLLWQISTGLGQNLPTGFLCLIKL*
NHRG*WWVTLRISTR*SSSRRQQCRIWNRGVPS
WKNSLPLPKI*KTRPAIKRLEQSLRIELKEFRIS
GMKYKNTFRTGGNS*MKC*RIQHNGWKLRKKLS
RS*DRPEPSLSHGRRVPIQ*MQSKRKSQKPSSW
PKTSASGRQM*MWQMTWP*NFSGIILQMIPEKST
**QRISMPLGEAFIKG*VSERLLWKKLIDYCNS
SPWTWKSFLPGLQKLKQLPMSYRMLPVRKGS*K
TPRE*KS**NNGKTSKVKLKLTQMFITTWMKTAK
KS*DPWKVPMMQSCYKDVWIT*TSSGVNFGKSL
STLGPIWKPVLTSGSVCTFLCRNFWCGYS*KMM
N*AGRHLLEATFQQFRSRTMYIGPSRGN*KLKNL
*S*VLLRLYEYF*QSSLWKD*RNSTRSPESCLL
RREPRMSLGFYESRLRRSILSGKN*TCTPLTGR
EK*MRPLKDSGNFKRPRMSWTSSCAKLR*SRDPG
SPWAISSLTLSKITSRKSRHFEEKLRL*KRT*A
TSMTLLASLPLWAFSSHRITSALWKT*TPDGSF
CRWPSRTESGSCMKPTGTLVQHLSTFFPRLSRVP
GREPSRQTKCPTISTTRLKQLAGTIPK*QSSTS
L*LT*IMSDSQLIGLP*NSEDCRRPFAWIS*AC
QLHVMPWTSTTSSKMTSPWISCRLLIV*PLFMTA
WSKSTTIWSTSLSAWICV*TGC*MFMIRDEQGG
SVSCLLKLASFPCVKHIWKTSTDTFSSKWQVQQ
DFVTSAGWASFCMILSKFQDSWVKLHPLGAVTLS
QVSGAASNLLIISQRSKRPSS*TG*DWNPSPWC
GCPSCTEWLLQKLPSIRPNVTSAKSVQSLDSGT
GV*STLIMTSAKAAFFLVELQKAIKCTIPWWNIA
LRLHQEKMFETLPRY*KTNFEPKGILRSIPEWA
TCQCRLS*RGTTWKLPLL*STSGQ*ILRLPRPL
SFHTMILIHALNIMLAG*QKWKTAMDLI*MIASL
LMRA*MMNIC*SSITAKV*TRTPP*ASLVVLPR
S*FP*RVRKEGS*RES*QILRKKTGICKQNMTV
*SSSTNIKACPHCRPLLK*CPPLPRVPGMLSSLL
RPSYCVNTKAAWKPGCKSWKTTINSWSHSYTG*
GSCWSNPRQRPK*MAQRCPLLLPLYRGPTAVSL
CCSEWLAVKLRTPWVRKIFSVLPRTQAQG*RR*W
SNSTTPSLVQEEEIPLESQ*ERTQCRKSFPHGR
*FGQSDGVLSISHDR*RRSRINVLQLLIPAWFL
*YSYNKED*TVRVYKK*IYIFVKGSGIIL*ISVV
SKSVIVLLTMAGFTRLCNCTKKL*ENYM*NLDS
*ITCHFFIWNAFWVV*KFITVIKKDCKLKCAL*
KKVVYKNP*KQNKHTHTHIHTHTQNFEAAHCFAS
FWRDIHMKFMAFSFFAY*R*DFLYHHTK*LLHT
AHLRTVS*VGQA*VFISYIYMSISI*IL*LYR*
RDTNFYRLTFSIF*MFMSHPNRKKLLLVSHPGLP
AWSRMDFSRSRKPGGNYTTLKHCLQLQMFLILN
NFPLTTKVK*SIGFF*REHVNEYTGLIISE*VI
GWLVD*LID*YIQLPAASNATI*I**CFSGNQSE
GILTL*TSEGIF*LPSSSRTMIGLEGYGFPAHP
CEGVGHSLSEGLDDCS*YIKFSVITTKLLCPLL
TVKRNWVVWFLLLF*IYCPMWDEFLNATRHNLK*
INFGKRCKTVAPSHL*Y*QVSTQKPMNCVSILC
ISLRVVPHRFVSK*ERRQIDSNVTKKPFLVD*T
G*IYKQTNKNCSKKRRKAQEEKLRTGRKKLYSFM
PFYFFLIFKSFIQ*IPPCDL*FCKSVTSDIKCN
*LLESGLTSSVISFWRVGFVHY**LIN*HQTRL
LMLFLPHFGFGVFLIIVHT*VHSFTTCPLRYFLF
PL*FFLFPS*FSKENPKL*GNKLPNYMKIWFLS
CIFFLYVTLDLFFTQGFLKLRFKTRGYFTSY*E
V*VSKFHSKIRGK*SA*IILF*SFCFSFRHISSG
VSLS*YLNKN*ELYCCILSIINLDIISCCVLYN
HQVLNCKS*CN*SINITWHVLSLFSGTEFLLEY
HNILCFNTNTVTFTNYFFKLQFYCIFTTYQTSPN
ICLTIVL*YCFTVYLNKARSYV
Frame 1
VKCKRCSGSCVCSEV*VLFD*RIR*SEWLPHRRCSSCIIND*KNHVRHAHLT*LESCERGAVGIQI
DFLVPSSRQ**NAFRKMTESGERCCFALS*FVTA
ANLLA*WSDRKNSWHGR*KRRCSKENIHKMGKC
TIF*VWEAAY*EPLQ*PTGWEAPPRPPRRPDRA
KTAKRKRIHKSSCPEQCQQGTAGFAEQ*C*FSEY
WKY*HRRWKS*TDSWFDLEYNPPLAGQKCNEKY
HGWIATNQQ*KDSPELGPTINS*LSTG*CNQLH
HQLV*WPGFECSHP*S*ARPI*LE*CGLPAVSHT
TTGTCIQHRQISIRHRETTRS*RC*YHLSR*EV
HLNVHHITLPSFASTSEH*SHPGSGNVAKAT*S
D*RRTFSVTSSNALFSTDHGQSSTGI*ENFFP*A
SIQELCLHTGCLCHHL*PYTEPISFTAFGSS*R
QVIWQFIDGE*SKPGPLSNSFRRSIIVASFC*G
HIASTRRDF**CGSGERPVSYS*GVHDGFDSPSG
PGW*YSTIGK*ADWNRKIIRR*RN*STRADESP
KFKMGMPQGS*HGKTKQFT*SFNGSPESETERV
E*LANKNRRKNKENGGRASWT*S*RPKTPSTTT*
GASRRSRTRTSQGQFSHSHGGGS**I*WRSRNC
CFGRTT*GIGRSMGKHL*MDRRPLGSFTRHPSQ
MATSY*RTVPF*CMAFRKRRCSEQDSHNWL*RSK
*NVIKSSKTGRFKSGSRKEKAIHGQTVFTQTRS
SFNTEE*VSDPEDGSMAG*LCPVLG*FSPKT*K
EYSTDFTGCHHHSAITNTDNCNGNSNYGDHKGTD
PGKACSRGTSTTTSPKEEADYCGF*N*EKVGC*
YN*TSQLDYSLRSCVAES*ICNLSEGRQLLRLK
RKSQCHRARKS*EVQKTARCQQISSGPGGTDGE*
GC*CR*HQTSLRTTEQPVDRILPVAK*ET*LAG
VSEQHHRFL*SATTIGADDNYC*KLVENPTHHP
IRANSN*KSVKNL*G*SQPAIRSSTSN*TIKNSK
HSPERERTRTHVPGCRLCGLYKSF*ASLF*CAG
QRERATDNF*HFATNALSGDHECHQDMGPAVRN
QTLHTST*CHRL*NHGAETRGIAGFTKFSARATK
WPILSQHHCERDVEESAL*N*PEISIRI*RN*G
TLEEALLPAG*ALSKARGANE*TPKNSESHTNP
EEMDG*S*CFSEGGMACPWGFRNSKKAAETVQTF
SQ*YSDNSAQSKQCQ*RWAEDKE*SRARVCFET
*DRTQRT*HSVGSHVPTGLCQKGGLEGRFGENC
KPPERSIRDARMDDTS*RRVS*ERF*I*NSR*IT
ESS*RDEES*RRGPTKRSESETPY*VCK*CHSS
SSTCSTRGLKKGT*NSNHQLPVALH*AEWEMQD
FGRSLGMLA*VIVILGESKQVAK*SRI*T*NH*K
HSWRS*GNL*GARFT*KFDATFRG*PKSDSHIG
TDPNRWRSHG*ANQ*GT*DI*FSLEGTT*RGCK
EAKVA*TEHPVCPGD*KILTLNPGVPHIH*QAVG
SLYCRQGGRSSNASGSPENPI*FDKS*DQFRRN
EET*SGEGGCPKSPVSD*CCTEKITRCLHEVSI
IPETSQF*AASTRK*DDFR*SEDALACIGNKECG
TGSSTVTAKSLCELV*KSE*SEV*SGNGDKDWT
SDCTEKADGKSQRT**KSNSFEIAL**AGSKGN
RKKATVGEMLEIVP*DAKGNECLDRMAGSYRYGI
DKEISS*RNA**FGF*SCLGKGYSKRD*ETEGA
PEEYHRGRRGLENSFGQEGDVGGR*TQSSE**L
DSCHLPSRRVVKSFVGIPETHGNF*PECGPHHKV
DHSG*HTFG*IREKETPAKRRRA*AFKGRTE*H
TPKGGLYT*PSSKLDGKPR*PLQEISRAPNLRA
QPSICSHFTQN*DWKGLHSFEGIGAV*LRYTKIA
*TTGG*NSAGGESERGRLQ*RYE*RQ*GYCKRI
VAKRRQLTTKNHR*EKARGNKDKTAAVTDKT*C
SQGFEVSKKKKGSRNFSSVVSVQEAG**SPEMLG
*H*KKISQPT*AQR*KENKGN*SGIAEEERGAE
CSA*AS*GLV*GWGRNGSGANSDPAQQALAGN*
EQICSVSKTQLCTNSHCP*RNDDGDD*RHAFGNF
LCAFYLFD*NHSCLTSPIRSGTTSQCS*PLC*G
L*RSL*ARGVSEEYKR*STTKLRSD*HYS*QED
SSIAKCNACGKGEATGSSLPA*FPMGKS*QNVQG
PTRAI*QIC*EMAAFSL*YKDI*SVANRS*TVS
QKDTNS*ELGTC*IQMVS*GTPGWHWAAANCCQ
NIECNWGRNNSAILKNRCQYSTGKIGKPESAVAG
GLQTAVRQKKEARRTKEYLVRISKRFK*ICFMV
GGSR*HC*YPT*TWKRAATKRKA*ASQVTGGRV
APAPGNSQTIK*NWRTRACKCSHKPRRAR*T*K*
AQADKSPVDKGFQSFT*ETRRN*SSNKRPWAA*
KKA*RP*RAVKSSAAVVISY*ESVGNL*PTKPR
RTI*R*GN*NSSSS*TTGCGRDFV*RAAFVQGKT
SHSASEEEVRRSEL*VEGGKPFTSRAEGKAA*P
SSWTDHYWSLSYSDCYSGDTTCGY*GNCHLQTR
NAIFLDVGGTCSGRFQPGLDRTYRLAFSA*SSYK
ITEGDGG*P*GYQRDDHQAEGNNAGFGTEASPV
GRTHYRCPKFEKQDQQSRG*NNHYGSN*KNSES
VG*STRTPSEPEATVE*NVKGFNTMAGS*GRS*A
GLRTGQSQA*VMEGGSLYSRCNPKENHRNQAVG
QRPPPVADKCRCGK*LGPETSPGLFCR*YQKSP
HDNREYQCLLEKHS*KGE*ARGCFGRNS*ITATV
PPGPGKVSCLAYRS*NNCQCPTGCYP*GKAPRR
LQGSKRADETMARPPR*N*SSHRCLSQPG*KQP
KNPEIPGRFR*CSPVTKTFG*HELQVE*TSEKVS
QH*VPFGSQF*PVEASAPFSAGTSGVATAER**
IKPAGTYWRRLSSSSEAERCT*GLQEGIEN*RT
CNHEYS*DCTNISDRAAFGRTRETLPGAQRAAS*
GESPECHSASTKAG*GGQY*VGKIEPALR*LAE
KNR*DP*KTPGTSRGHG*AGPQAAPS*GDQGIL
AARGRSPH*LSPRSPRESQGTSRRNCASEREREP
RQ*PCSPAYHFGHSALTV*PQHSGRPEHQMEAS
AGGRRGPSQAAA*SPQGLWSSISALSFHVCPGS
LGESHLAKQSALLYQPRDSNNLLGPSQNDRALPV
FS*PE*CQILSL*DCHETPKTAEGPLLGSLEPV
SCM*CLGPAQPQAK*PAHGYPADY*LFDHYL*P
PGARAQQFGQRPSLRGYVSELAAECL*YGTNRED
PCPVF*NWHHFPV*STFGRQVQIPFQASGKFNR
IL*PAQAGPPSA*FYPNSKTVG*SCILWGQ*H*
AKCPELLPIC***ARDRSGPLPRLDETGTPVHGV
AARPAQSGCCRNCQASGQM*HLQRVSNHWIQVQ
ESKAL*L*HLPKLLFFWSSCKRP*NALSHGGIL
HSDYIRRRCSRLCQGTKKQISNQKVFCEASPNGL
PASADCLRGGQHGNSRYSDQLLASRFCACLVPS
AFTR*YSFTH*TLC*QASRNGKQQWILSK**HL
S**EHR**TFVNPALLPKFEPGLPPEPAS*SCPD
LDFLRE*GKRGARENPSRS*GRKQESASRI*PS
KAAART*RPVPTAVPS*NDAHLSPESPGC*AHC
*GQATASTQRPPGSQDANPGRPQ*TAGVTVTQAK
AAAGATPGRGQSEWHNGVLSFYLSTEVRQQSAY
AAPSGWQSNFGLHG*GRSSQSSPGHKHRVRGGD
GATQQLLP*FKRKKYPWKANERGHNVGSLFHMAD
DLGRAMESLVSVMTDEEGAE*MFYNS*FPHGFY
NIHTTKRIRQ*EFTRNKSIFL*RVVVLYCRFQ*
FLSLLLFC*QWQVLHVYAIVQKSYKKTTCKILIA
K*LAISLYGTHFGLFKNL*QL*RKIVN*SVLYK
KKLFIKTPKNKTNTHTHTYTHTHKTLRQRIVLH
PFGVISI*NSWLFLFLHIKDKTSSTTTPNDYYTL
LI*ELSAEWGRLEFSFHISICL*VYKYYSYIDK
EIRISID*LFPFFKCSCHILIERNYF*SVIQAY
LLGLEWIFPGAGSQEETTPH*NIVYSSRCFSF*T
TFH*QRK*SKVLDFFKGNM*MNTQDLLYQSE*S
VGWLID*LIDTFSFLLLAMPRFRFNDASVEINQ
KVF*PCEHQKVFFNSQAVAGR**GWRAMDSQPIP
VKE*ATL*VKDWMIVHNT*SSL*LQLNYYALFS
QSKGTGWFGFCCFFRFIVPCGMSF*MPQDII*N
K*TLGKGVKQ*PHHICDTDRYQPRSP*TVFPSFA
FLCE*FHTGL*VSKKEGKLIQMLQKNPSWWIRQ
VKYINKQTKIAQKRGEKLKRKS*GLVGKSFTLS
CHFISF*FLNHSFNRYHRVTYNFANLLPLTSSVI
SFWRVG*HQV*LAFGEWVLSIINN*LINIKHGF
SCYFYLTLVLGCS**LCTPEFTASPLVHCVIFF
FLYNSFFFLHNFQKKTQSSKVTNYQIT*RFGFCL
AFFSFM*RWTFSLPKDF*NSDLKQGVTLHPTKK
FK*VSFILKSEVNRVHK*FCFNLFVFLLDTLAL
E*VCHNI*TKIESFIAAF*A*LIWTLFRVVFFIT
TKY*TVNHNVTEA*TSHGMFCHCFQVLSSYLSI
IIYCVLTPTL*HLRIIFLNFSFTAFSQHIRLHQ
IYALLLYYSTALLCISIKHAVML
Frame 2
LNANAALAHVFAPRYRFCSTDVSDSQSGYHTDVVAAA**MTERIMLGMPT*PNLNHAKGELLEFK*
TFWFPAVGSNRMLSGR*QNQEKDAVLHYLDLLQ
QPTYWHDGVTGKTAGMEDEREDVQKKTFTKWVNA
QFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQ
KLPKEKGSTRVHALNNVNKALRVLQNNNVDLVN
IGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNI
MAGLQQTNSEKILLSWVRQSTRNYPQVNVINFT
TSWSDGLALNALIHSHRPDLFDWNSVVCQQSAT
QRLEHAFNIARYQLGIEKLLDPEDVDTTYPDKKS
ILMYITSLFQVLPQQVSIEAIQEVEMLPRPPKV
TKEEHFQLHHQMHYSQQITVSLAQGYERTSSPK
PRFKSYAYTQAAYVTTSDPTRSPFPSQHLEAPED
KSFGSSLMESEVNLDRYQTALEEVLSWLLSAED
TLQAQGEISNDVEVVKDQFHTHEGYMMDLTAHQ
GRVGNILQLGSKLIGTGKLSEDEETEVQEQMNLL
NSRWECLRVASMEKQSNLHRVLMDLQNQKLKEL
NDWLTKTEERTRKMEEEPLGPDLEDLKRQVQQH
KVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATA
ALEEQLKVLGDRWANICRWTEDRWVLLQDILLK
WQRLTEEQCLFSAWLSEKEDAVNKIHTTGFKDQ
NEMLSSLQKLAVLKADLEKKKQSMGKLYSLKQDL
LSTLKNKSVTQKTEAWLDNFARCWDNLVQKLEK
STAQISQAVTTTQPSLTQTTVMETVTTVTTREQ
ILVKHAQEELPPPPPQKKRQITVDSEIRKRLDVD
ITELHSWITRSEAVLQSPEFAIFRKEGNFSDLK
EKVNAIEREKAEKFRKLQDASRSAQALVEQMVN
EGVNADSIKQASEQLNSRWIEFCQLLSERLNWLE
YQNNIIAFYNQLQQLEQMTTTAENWLKIQPTTP
SEPTAIKSQLKICKDEVNRLSDLQPQIERLKIQ
SIALKEKGQGPMFLDADFVAFTNHFKQVFSDVQA
REKELQTIFDTLPPMRYQETMSAIRTWVQQSET
KLSIPQLSVTDYEIMEQRLGELQALQSSLQEQQ
SGLYYLSTTVKEMSKKAPSEISRKYQSEFEEIEG
RWKKLSSQLVEHCQKLEEQMNKLRKIQNHIQTL
KKWMAEVDVFLKEEWPALGDSEILKKQLKQCRL
LVSDIQTIQPSLNSVNEGGQKIKNEAEPEFASRL
ETELKELNTQWDHMCQQVYARKEALKGGLEKTV
SLQKDLSEMHEWMTQAEEEYLERDFEYKTPDEL
QKAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQ
APPVAQEALKKELETLTTNYQWLCTRLNGKCKT
LEEVWACWHELLSYLEKANKWLNEVEFKLKTTE
NIPGGAEEISEVLDSLENLMRHSEDNPNQIRILA
QTLTDGGVMDELINEELETFNSRWRELHEEAVR
RQKLLEQSIQSAQETEKSLHLIQESLTFIDKQL
AAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEM
KKHNQGKEAAQRVLSQIDVAQKKLQDVSMKFRL
FQKPANFEQRLQESKMILDEVKMHLPALETKSV
EQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGR
QIVQKKQTENPKELDERVTALKLHYNELGAKVT
ERKQQLEKCLKLSRKMRKEMNVLTEWLAATDME
LTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVH
LKSITEVGEALKTVLGKKETLVEDKLSLLNSNW
IAVTSRAEEWLNLLLEYQKHMETFDQNVDHITK
WIIQADTLLDESEKKKPQQKEDVLKRLKAELNDI
RPKVDSTRDQAANLMANRGDHCRKLVEPQISEL
NHRFAAISHRIKTGKASIPLKELEQFNSDIQKL
LEPLEAEIQQGVNLKEEDFNKDMNEDNEGTVKEL
LQRGDNLQQRITDERKREEIKIKQQLLQTKHNA
LKDLRSQRRKKALEISHQWYQYKRQADDLLKCL
DDIEKKLASLPEPRDERKIKEIDRELQKKKEELN
AVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIE
SKFAQFRRLNFAQIHTVREETMMVMTEDMPLEI
SYVPSTYLTEITHVSQALLEVEQLLNAPDLCAKD
FEDLFKQEESLKNIKDSLQQSSGRIDIIHSKKT
AALQSATPVERVKLQEALSQLDFQWEKVNKMYK
DRQGRFDRSVEKWRRFHYDIKIFNQWLTEAEQFL
RKTQIPENWEHAKYKWYLKELQDGIGQRQTVVR
TLNATGEEIIQQSSKTDASILQEKLGSLNLRWQ
EVCKQLSDRKKRLEEQKNILSEFQRDLNEFVLWL
EEADNIASIPLEPGKEQQLKEKLEQVKLLVEEL
PLRQGILKQLNETGGPVLVSAPISPEEQDKLEN
KLKQTNLQWIKVSRALPEKQGEIEAQIKDLGQLE
KKLEDLEEQLNHLLLWLSPIRNQLEIYNQPNQE
GPFDVKETEIAVQAKQPDVEEILSKGQHLYKEK
PATQPVKRKLEDLSSEWKAVNRLLQELRAKQPDL
APGLTTIGASPTQTVTLVTQPVVTKETAISKLE
MPSSLMLEVPALADFNRAWTELTDWLSLLDQVI
KSQRVMVGDLEDINEMIIKQKATMQDLEQRRPQL
EELITAAQNLKNKTSNQEARTIITDRIERIQNQ
WDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAE
QVLGQARAKLESWKEGPYTVDAIQKKITETKQLA
KDLRQWQTNVDVANDLALKLLRDYSADDTRKVH
MITENINASWRSIHKRVSEREAALEETHRLLQQ
FPLDLEKFLAWLTEAETTANVLQDATRKERLLED
SKGVKELMKQWQDLQGEIEAHTDVYHNLDENSQ
KILRSLEGSDDAVLLQRRLDNMNFKWSELRKKS
LNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDE
LSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEP
VIMSTLETVRIFLTEQPLEGLEKLYQEPRELPP
EERAQNVTRLLRKQAEEVNTEWEKLNLHSADWQR
KIDETLERLRELQEATDELDLKLRQAEVIKGSW
QPVGDLLIDSLQDHLEKVKALRGEIAPLKENVS
HVNDLARQLTTLGIQLSPYNLSTLEDLNTRWKLL
QVAVEDRVRQLHEAHRDFGPASQHFLSTSVQGP
WERAISPNKVPYYINHETQTTCWDHPKMTELYQ
SLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLS
AACDALDQHNLKQNDQPMDILQIINCLTTIYDR
LEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGR
IRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTG
FCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIE
PSVRSCFQFANNKPEIEAALFLDWMRLEPQSMV
WLPVLHRVAAAETAKHQAKCNICKECPIIGFRYR
SLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYC
TPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMG
YLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQ
LSHDDTHSRIEHYASRLAEMENSNGSYLNDSIS
PNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQ
ILISLESEERGELERILADLEEENRNLQAEYDRL
KQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIA
EAKLLRQHKGRLEARMQILEDHNKQLESQLHRL
RQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPM
LLRVVGSQTSDSMGEEDLLSPPQDTSTGLEEVM
EQLNNSFPSSRGRNTPGKPMREDTM*EVFSTWQ
MIWAERWSP*YQS*QMKKEQNKCFTTPDSRMVFI
IFIQQRGLDSKSLQEINLYFCEG*WYYTVDFSS
F*VCYCFVNNGRFYTSMQLYKKVIRKLHVKS**
LNNLPFLYMERILGCLKIYNSYKERL*TKVCFIK
KSCL*KPLKTKQTHTHTHTHTHTKL*GSALFCI
LLA*YPYEIHGFFFFCILKIRLPLPPHQMTTTH
CSFENCQLSGAGLSFHFIYLYVYKYINTIVI*IK
RYEFL*TDFFHFLNVHVTS**KEITSSQSSRLT
CLV*NGFFPEPEARRKLHHTKTLSTAPDVSHFK
QLSTDNESKVKYWIFLKGTCE*IHRTYYIRVSNR
LVG*LID*LIHSASCC*QCHDLDLMMLQWKSIR
RYSDLVNIRRYFLTPKQ*QDDDRAGGLWIPSPS
L*RSRPLFK*RIG*LFIIHKVLCNYN*IIMPSSH
SQKELGGLVFVAFLDLLSHVG*VFKCHKT*FKI
NKLWEKV*NSSPITFVILTGINPEAHELCFHPL
HFSASSSTQVCK*VRKKAN*FKCYKKTLLGGLDR
LNI*TNKQKLLKKEEKSSRGKAKDW*EKALLFH
AILFLFDF*IIHSIDTTV*PIILQICYL*HQV*
LAFGEWADIKCN*LLESGFCPLLIIN*LTSNTAS
HAISTSLWFWGVPDNCAHLSSQLHHLSIALFSF
SFIILSFSFIIFKRKPKALR*QITKLHEDLVFV
LHFFPLCDAGPFLYPRIFKTQI*NKGLLYILLRS
LSK*VSF*NQR*IECINNFVLIFLFFF*TH*LW
SESVIIFEQKLRALLLHFKHN*FGHYFVLCSL*
PPSIKL*IIM*LKHKHHMACFVIVFRY*VLT*VS
*YIVF*HQHCNIYELFF*TSVLLHFHNISDFTK
YMPYYCIIVLLYCVSQ*STQLCY
Sequence NM_004011
TAATGAGATCAGGAGGAACATTCGACCTGAGAAAGACAGATTGCAATGACTGAGATGATTTTGCTAATTTTTTTTCCAGCCTATTTCCTTAATGCTGTAAGGAGGCAAAAGTTGCTTGAACAGAGCATCCAGTCTGCCCAGGAGACTGAAAAATCCTTACACTTAATCCAGGAGTCCCTCACATTCATTGACAAGCAGTT
GGCAGCTTATATTGCAGACAAGGTGGACGCAGCTCAAATGCCTCAGGAAGCCCAGAAAATCCAATCTGATTTGACAAGTCATGAGATCAGTTTAGAAGAA
ATGAAGAAACATAATCAGGGGAAGGAGGCTGCCCAAAGAGTCCTGTCTCAGATTGATGTTGCACAGAAAAAATTACAAGATGTCTCCATGAAGTTTCGAT
TATTCCAGAAACCAGCCAATTTTGAGCAGCGTCTACAAGAAAGTAAGATGATTTTAGATGAAGTGAAGATGCACTTGCCTGCATTGGAAACAAAGAGTGT
GGAACAGGAAGTAGTACAGTCACAGCTAAATCATTGTGTGAACTTGTATAAAAGTCTGAGTGAAGTGAAGTCTGAAGTGGAAATGGTGATAAAGACTGGA
CGTCAGATTGTACAGAAAAAGCAGACGGAAAATCCCAAAGAACTTGATGAAAGAGTAACAGCTTTGAAATTGCATTATAATGAGCTGGGAGCAAAGGTAA
CAGAAAGAAAGCAACAGTTGGAGAAATGCTTGAAATTGTCCCGTAAGATGCGAAAGGAAATGAATGTCTTGACAGAATGGCTGGCAGCTACAGATATGGA
ATTGACAAAGAGATCAGCAGTTGAAGGAATGCCTAGTAATTTGGATTCTGAAGTTGCCTGGGGAAAGGCTACTCAAAAAGAGATTGAGAAACAGAAGGTG
CACCTGAAGAGTATCACAGAGGTAGGAGAGGCCTTGAAAACAGTTTTGGGCAAGAAGGAGACGTTGGTGGAAGATAAACTCAGTCTTCTGAATAGTAACT
GGATAGCTGTCACCTCCCGAGCAGAAGAGTGGTTAAATCTTTTGTTGGAATACCAGAAACACATGGAAACTTTTGACCAGAATGTGGACCACATCACAAA
GTGGATCATTCAGGCTGACACACTTTTGGATGAATCAGAGAAAAAGAAACCCCAGCAAAAAGAAGACGTGCTTAAGCGTTTAAAGGCAGAACTGAATGAC
ATACGCCCAAAGGTGGACTCTACACGTGACCAAGCAGCAAACTTGATGGCAAACCGCGGTGACCACTGCAGGAAATTAGTAGAGCCCCAAATCTCAGAGC
TCAACCATCGATTTGCAGCCATTTCACACAGAATTAAGACTGGAAAGGCCTCCATTCCTTTGAAGGAATTGGAGCAGTTTAACTCAGATATACAAAAATT
GCTTGAACCACTGGAGGCTGAAATTCAGCAGGGGGTGAATCTGAAAGAGGAAGACTTCAATAAAGATATGAATGAAGACAATGAGGGTACTGTAAAAGAA
TTGTTGCAAAGAGGAGACAACTTACAACAAAGAATCACAGATGAGAGAAAGCGAGAGGAAATAAAGATAAAACAGCAGCTGTTACAGACAAAACATAATG
CTCTCAAGGATTTGAGGTCTCAAAGAAGAAAAAAGGCTCTAGAAATTTCTCATCAGTGGTATCAGTACAAGAGGCAGGCTGATGATCTCCTGAAATGCTT
GGATGACATTGAAAAAAAATTAGCCAGCCTACCTGAGCCCAGAGATGAAAGGAAAATAAAGGAAATTGATCGGGAATTGCAGAAGAAGAAAGAGGAGCTG
AATGCAGTGCGTAGGCAAGCTGAGGGCTTGTCTGAGGATGGGGCCGCAATGGCAGTGGAGCCAACTCAGATCCAGCTCAGCAAGCGCTGGCGGGAAATTG
AGAGCAAATTTGCTCAGTTTCGAAGACTCAACTTTGCACAAATTCACACTGTCCGTGAAGAAACGATGATGGTGATGACTGAAGACATGCCTTTGGAAAT
TTCTTATGTGCCTTCTACTTATTTGACTGAAATCACTCATGTCTCACAAGCCCTATTAGAAGTGGAACAACTTCTCAATGCTCCTGACCTCTGTGCTAAG
GACTTTGAAGATCTCTTTAAGCAAGAGGAGTCTCTGAAGAATATAAAAGATAGTCTACAACAAAGCTCAGGTCGGATTGACATTATTCATAGCAAGAAGA
CAGCAGCATTGCAAAGTGCAACGCCTGTGGAAAGGGTGAAGCTACAGGAAGCTCTCTCCCAGCTTGATTTCCAATGGGAAAAAGTTAACAAAATGTACAA
GGACCGACAAGGGCGATTTGACAGATCTGTTGAGAAATGGCGGCGTTTTCATTATGATATAAAGATATTTAATCAGTGGCTAACAGAAGCTGAACAGTTT
CTCAGAAAGACACAAATTCCTGAGAATTGGGAACATGCTAAATACAAATGGTATCTTAAGGAACTCCAGGATGGCATTGGGCAGCGGCAAACTGTTGTCA
GAACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGATGCCAGTATTCTACAGGAAAAATTGGGAAGCCTGAATCTGCGGTGGCA
GGAGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGGCTAGAAGAACAAAAGAATATCTTGTCAGAATTTCAAAGAGATTTAAATGAATTTGTTTTATGG
TTGGAGGAAGCAGATAACATTGCTAGTATCCCACTTGAACCTGGAAAAGAGCAGCAACTAAAAGAAAAGCTTGAGCAAGTCAAGTTACTGGTGGAAGAGT
TGCCCCTGCGCCAGGGAATTCTCAAACAATTAAATGAAACTGGAGGACCCGTGCTTGTAAGTGCTCCCATAAGCCCAGAAGAGCAAGATAAACTTGAAAA
TAAGCTCAAGCAGACAAATCTCCAGTGGATAAAGGTTTCCAGAGCTTTACCTGAGAAACAAGGAGAAATTGAAGCTCAAATAAAAGACCTTGGGCAGCTT
GAAAAAAAGCTTGAAGACCTTGAAGAGCAGTTAAATCATCTGCTGCTGTGGTTATCTCCTATTAGGAATCAGTTGGAAATTTATAACCAACCAAACCAAG
AAGGACCATTTGACGTTAAGGAAACTGAAATAGCAGTTCAAGCTAAACAACCGGATGTGGAAGAGATTTTGTCTAAAGGGCAGCATTTGTACAAGGAAAA
ACCAGCCACTCAGCCAGTGAAGAGGAAGTTAGAAGATCTGAGCTCTGAGTGGAAGGCGGTAAACCGTTTACTTCAAGAGCTGAGGGCAAAGCAGCCTGAC
CTAGCTCCTGGACTGACCACTATTGGAGCCTCTCCTACTCAGACTGTTACTCTGGTGACACAACCTGTGGTTACTAAGGAAACTGCCATCTCCAAACTAG
AAATGCCATCTTCCTTGATGTTGGAGGTACCTGCTCTGGCAGATTTCAACCGGGCTTGGACAGAACTTACCGACTGGCTTTCTCTGCTTGATCAAGTTAT
AAAATCACAGAGGGTGATGGTGGGTGACCTTGAGGATATCAACGAGATGATCATCAAGCAGAAGGCAACAATGCAGGATTTGGAACAGAGGCGTCCCCAG
TTGGAAGAACTCATTACCGCTGCCCAAAATTTGAAAAACAAGACCAGCAATCAAGAGGCTAGAACAATCATTACGGATCGAATTGAAAGAATTCAGAATC
AGTGGGATGAAGTACAAGAACACCTTCAGAACCGGAGGCAACAGTTGAATGAAATGTTAAAGGATTCAACACAATGGCTGGAAGCTAAGGAAGAAGCTGA
GCAGGTCTTAGGACAGGCCAGAGCCAAGCTTGAGTCATGGAAGGAGGGTCCCTATACAGTAGATGCAATCCAAAAGAAAATCACAGAAACCAAGCAGTTG
GCCAAAGACCTCCGCCAGTGGCAGACAAATGTAGATGTGGCAAATGACTTGGCCCTGAAACTTCTCCGGGATTATTCTGCAGATGATACCAGAAAAGTCC
ACATGATAACAGAGAATATCAATGCCTCTTGGAGAAGCATTCATAAAAGGGTGAGTGAGCGAGAGGCTGCTTTGGAAGAAACTCATAGATTACTGCAACA
GTTCCCCCTGGACCTGGAAAAGTTTCTTGCCTGGCTTACAGAAGCTGAAACAACTGCCAATGTCCTACAGGATGCTACCCGTAAGGAAAGGCTCCTAGAA
GACTCCAAGGGAGTAAAAGAGCTGATGAAACAATGGCAAGACCTCCAAGGTGAAATTGAAGCTCACACAGATGTTTATCACAACCTGGATGAAAACAGCC
AAAAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTACAAAGACGTTTGGATAACATGAACTTCAAGTGGAGTGAACTTCGGAAAAAGTC
TCTCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCGTCTGCACCTTTCTCTGCAGGAACTTCTGGTGTGGCTACAGCTGAAAGATGAT
GAATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAGAAGCAGAACGATGTACATAGGGCCTTCAAGAGGGAATTGAAAACTAAAGAAC
CTGTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGCAGCCTTTGGAAGGACTAGAGAAACTCTACCAGGAGCCCAGAGAGCTGCCTCC
TGAGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGAGGAGGTCAATACTGAGTGGGAAAAATTGAACCTGCACTCCGCTGACTGGCAG
AGAAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCCACGGATGAGCTGGACCTCAAGCTGCGCCAAGCTGAGGTGATCAAGGGATCCT
GGCAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCGAGAAAGTCAAGGCACTTCGAGGAGAAATTGCGCCTCTGAAAGAGAACGTGAG
CCACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCTCTCACCGTATAACCTCAGCACTCTGGAAGACCTGAACACCAGATGGAAGCTT
CTGCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCACAGGGACTTTGGTCCAGCATCTCAGCACTTTCTTTCCACGTCTGTCCAGGGTC
CCTGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACCACGAGACTCAAACAACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCA
GTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGCCATGAAACTCCGAAGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTG
TCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGACCAGCCCATGGATATCCTGCAGATTATTAATTGTTTGACCACTATTTATGACC
GCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGGATATGTGTCTGAACTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAG
GATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGCACATTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACA
GGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATCCAAATTCCAAGACAGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTG
AGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGATCGAAGCGGCCCTCTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGT
GTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCATCAGGCCAAATGTAACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTAC
AGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGTCGAGTTGCAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATT
GCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTAAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGG
CTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCCCGTTACTCTGATCAACTTCTGGCCAGTAGATTCTGCGCCTGCCTCGTCCCCT
CAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATCTAAATGATAGCATCT
CTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTTTGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCA
GATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAAACAGGAATCTGCAAGCAGAATATGACCGT
CTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATGATGCCCACCTCTCCCCAGAGTCCCCGGGATGCTGAGCTCATTG
CTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCCTGGAAGACCACAATAAACAGCTGGAGTCACAGTTACACAGGCT
AAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTCCTCTCCTTCTACCTCTCTACAGAGGTCCGACAGCAGTCAGCCT
ATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTTCTCAGTCCTCCCCAGGACACAAGCACAGGGTTAGAGGAGGTGA
TGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCCCTGGAAAGCCAATGAGAGAGGACACAATGTAGGAAGTCTTTTCCACATGGCA
GATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGAGCAGAATAAATGTTTTACAACTCCTGATTCCCGCATGGTTTTT
ATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTATATTTTTGTGAAGGGTAGTGGTATTATACTGTAGATTTCAGTA
GTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTTATAAGAAAACTACATGTAAAATCTTGATA
GCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAGAAAGATTGTAAACTAAAGTGTGCTTTATA
AAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACATACACACACACACACAAAACTTTGAGGCAGCGCATTGTTTTGCA
TCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAAAGATAAGACTTCCTCTACCACCACACCAAATGACTACTACACA
CTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATAAGTATATAAATACTATAGTTATATAGATA
AAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGAAATTACTTCTAGTCAGTCATCCAGGCTTA
CCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACCACACTAAAACATTGTCTACAGCTCCAGATGTTTCTCATTTTAA
ACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAACATGTGAATGAATACACAGGACTTATTATATCAGAGTGAGTAAT
CGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGATTTAATGATGCTTCAGTGGAAATCAATCA
GAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGGGCTGGAGGGCTATGGATTCCCAGCCCATC
CCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACATAAAGTTCTCTGTAATTACAACTAAATTATTATGCCCTCTTCT
CACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCCCATGTGGGATGAGTTTTTAAATGCCACAAGACATAATTTAAAA
TAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGGTATCAACCCAGAAGCCCATGAACTGTGTTTCCATCCTTT
GCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTTACAAAAAAACCCTTCTTGGTGGATTAGAC
AGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCAAGAGGAAAAGCTAAGGACTGGTAGGAAAAAGCTTTACTCTTTC
ATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACCGTGTGACCTATAATTTTGCAAATCTGTTACCTCTGACATCAAGTGTA
ATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAATAATTAATTAATTAACATCAAACACGGCT
TCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTT
TTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTCTAAGGTAACAAATTACCAAATTACATGAAGATTTGGTTTTTGT
CTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAAAACTCAGATTTAAAACAAGGGGTTACTTTACATCCTACTAAGA
AGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAGACACATTAGCTCT
GGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTTGGACATTATTTCGTGTTGTGTTCTTTATA
ACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGCATGTTTTGTCATTGTTTTCAGGTACTGAGTTCTTACTTGAGTA
TCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTTTTAAACTTCAGTTTTACTGCATTTTCACAACATATCAGACTTCACCA
AATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
**DQEEHST*ERQIAMTEMILLIFFPAYFLNAVRRQKLLEQSIQSAQETEKSLHLIQESLTFIDKQL
AAYIADKVDAAQMPQEAQKIQSDLTSHEISLEE
MKKHNQGKEAAQRVLSQIDVAQKKLQDVSMKFR
LFQKPANFEQRLQESKMILDEVKMHLPALETKSV
EQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTG
RQIVQKKQTENPKELDERVTALKLHYNELGAKV
TERKQQLEKCLKLSRKMRKEMNVLTEWLAATDME
LTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKV
HLKSITEVGEALKTVLGKKETLVEDKLSLLNSN
WIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITK
WIIQADTLLDESEKKKPQQKEDVLKRLKAELND
IRPKVDSTRDQAANLMANRGDHCRKLVEPQISE
LNHRFAAISHRIKTGKASIPLKELEQFNSDIQKL
LEPLEAEIQQGVNLKEEDFNKDMNEDNEGTVKE
LLQRGDNLQQRITDERKREEIKIKQQLLQTKHN
ALKDLRSQRRKKALEISHQWYQYKRQADDLLKCL
DDIEKKLASLPEPRDERKIKEIDRELQKKKEEL
NAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREI
ESKFAQFRRLNFAQIHTVREETMMVMTEDMPLEI
SYVPSTYLTEITHVSQALLEVEQLLNAPDLCAK
DFEDLFKQEESLKNIKDSLQQSSGRIDIIHSKK
TAALQSATPVERVKLQEALSQLDFQWEKVNKMYK
DRQGRFDRSVEKWRRFHYDIKIFNQWLTEAEQF
LRKTQIPENWEHAKYKWYLKELQDGIGQRQTVV
RTLNATGEEIIQQSSKTDASILQEKLGSLNLRWQ
EVCKQLSDRKKRLEEQKNILSEFQRDLNEFVLW
LEEADNIASIPLEPGKEQQLKEKLEQVKLLVEE
LPLRQGILKQLNETGGPVLVSAPISPEEQDKLEN
KLKQTNLQWIKVSRALPEKQGEIEAQIKDLGQL
EKKLEDLEEQLNHLLLWLSPIRNQLEIYNQPNQ
EGPFDVKETEIAVQAKQPDVEEILSKGQHLYKEK
PATQPVKRKLEDLSSEWKAVNRLLQELRAKQPD
LAPGLTTIGASPTQTVTLVTQPVVTKETAISKL
EMPSSLMLEVPALADFNRAWTELTDWLSLLDQVI
KSQRVMVGDLEDINEMIIKQKATMQDLEQRRPQ
LEELITAAQNLKNKTSNQEARTIITDRIERIQN
QWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAE
QVLGQARAKLESWKEGPYTVDAIQKKITETKQL
AKDLRQWQTNVDVANDLALKLLRDYSADDTRKV
HMITENINASWRSIHKRVSEREAALEETHRLLQQ
FPLDLEKFLAWLTEAETTANVLQDATRKERLLE
DSKGVKELMKQWQDLQGEIEAHTDVYHNLDENS
QKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKS
LNIRSHLEASSDQWKRLHLSLQELLVWLQLKDD
ELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKE
PVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPP
EERAQNVTRLLRKQAEEVNTEWEKLNLHSADWQ
RKIDETLERLRELQEATDELDLKLRQAEVIKGS
WQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVS
HVNDLARQLTTLGIQLSPYNLSTLEDLNTRWKL
LQVAVEDRVRQLHEAHRDFGPASQHFLSTSVQG
PWERAISPNKVPYYINHETQTTCWDHPKMTELYQ
SLADLNNVRFSAYRTAMKLRRLQKALCLDLLSL
SAACDALDQHNLKQNDQPMDILQIINCLTTIYD
RLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGR
IRVLSFKTGIISLCKAHLEDKYRYLFKQVASST
GFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNI
EPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMV
WLPVLHRVAAAETAKHQAKCNICKECPIIGFRY
RSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEY
CTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMG
YLPVQTVLEGDNMETPVTLINFWPVDSAPASSP
QLSHDDTHSRIEHYASRLAEMENSNGSYLNDSI
SPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQ
ILISLESEERGELERILADLEEENRNLQAEYDR
LKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELI
AEAKLLRQHKGRLEARMQILEDHNKQLESQLHRL
RQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQP
MLLRVVGSQTSDSMGEEDLLSPPQDTSTGLEEV
MEQLNNSFPSSRGRNTPGKPMREDTM*EVFSTWQ
MIWAERWSP*YQS*QMKKEQNKCFTTPDSRMVF
IIFIQQRGLDSKSLQEINLYFCEG*WYYTVDFS
SF*VCYCFVNNGRFYTSMQLYKKVIRKLHVKS**
LNNLPFLYMERILGCLKIYNSYKERL*TKVCFI
KKSCL*KPLKTKQTHTHTHTHTHTKL*GSALFC
ILLA*YPYEIHGFFFFCILKIRLPLPPHQMTTTH
CSFENCQLSGAGLSFHFIYLYVYKYINTIVI*I
KRYEFL*TDFFHFLNVHVTS**KEITSSQSSRL
TCLV*NGFFPEPEARRKLHHTKTLSTAPDVSHFK
QLSTDNESKVKYWIFLKGTCE*IHRTYYIRVSN
RLVG*LID*LIHSASCC*QCHDLDLMMLQWKSI
RRYSDLVNIRRYFLTPKQ*QDDDRAGGLWIPSPS
L*RSRPLFK*RIG*LFIIHKVLCNYN*IIMPSS
HSQKELGGLVFVAFLDLLSHVG*VFKCHKT*FK
INKLWEKV*NSSPITFVILTGINPEAHELCFHPL
HFSASSSTQVCK*VRKKAN*FKCYKKTLLGGLD
RLNI*TNKQKLLKKEEKSSRGKAKDW*EKALLF
HAILFLFDF*IIHSIDTTV*PIILQICYL*HQV*
LAFGEWADIKCN*LLESGFCPLLIIN*LTSNTA
SHAISTSLWFWGVPDNCAHLSSQLHHLSIALFS
FSFIILSFSFIIFKRKPKALR*QITKLHEDLVFV
LHFFPLCDAGPFLYPRIFKTQI*NKGLLYILLR
SLSK*VSF*NQR*IECINNFVLIFLFFF*TH*L
WSESVIIFEQKLRALLLHFKHN*FGHYFVLCSL*
PPSIKL*IIM*LKHKHHMACFVIVFRY*VLT*V
S*YIVF*HQHCNIYELFF*TSVLLHFHNISDFT
KYMPYYCIIVLLYCVSQ*STQLCY
Frame 1
NEIRRNIRPEKDRLQ*LR*FC*FFFQPISLML*GGKSCLNRASSLPRRLKNPYT*SRSPSHSLTSS
WQLILQTRWTQLKCLRKPRKSNLI*QVMRSV*KK
*RNIIRGRRLPKESCLRLMLHRKNYKMSP*SFD
YSRNQPILSSVYKKVR*F*MK*RCTCLHWKQRV
WNRK*YSHS*IIV*TCIKV*VK*SLKWKW**RLD
VRLYRKSRRKIPKNLMKE*QL*NCIIMSWEQR*
QKESNSWRNA*NCPVRCERK*MS*QNGWQLQIW
N*QRDQQLKECLVIWILKLPGERLLKKRLRNRRC
T*RVSQR*ERP*KQFWARRRRWWKINSVF*IVT
G*LSPPEQKSG*IFCWNTRNTWKLLTRMWTTSQ
SGSFRLTHFWMNQRKRNPSKKKTCLSV*RQN*MT
YAQRWTLHVTKQQT*WQTAVTTAGN**SPKSQS
STIDLQPFHTELRLERPPFL*RNWSSLTQIYKN
CLNHWRLKFSRG*I*KRKTSIKI*MKTMRVL*KN
CCKEETTYNKESQMRESERK*R*NSSCYRQNIM
LSRI*GLKEEKRL*KFLISGISTRGRLMIS*NA
WMTLKKN*PAYLSPEMKGK*RKLIGNCRRRKRS*
MQCVGKLRACLRMGPQWQWSQLRSSSASAGGKL
RANLLSFEDSTLHKFTLSVKKR*W**LKTCLWK
FLMCLLLI*LKSLMSHKPY*KWNNFSMLLTSVLR
TLKISLSKRSL*RI*KIVYNKAQVGLTLFIARR
QQHCKVQRLWKG*SYRKLSPSLISNGKKLTKCT
RTDKGDLTDLLRNGGVFIMI*RYLISG*QKLNSF
SERHKFLRIGNMLNTNGILRNSRMALGSGKLLS
EH*MQLGKK*FSNPQKQMPVFYRKNWEA*ICGG
RRSANSCQTEKRG*KNKRISCQNFKEI*MNLFYG
WRKQITLLVSHLNLEKSSN*KKSLSKSSYWWKS
CPCAREFSNN*MKLEDPCL*VLP*AQKSKINLK
ISSSRQISSG*RFPELYLRNKEKLKLK*KTLGSL
KKSLKTLKSS*IICCCGYLLLGISWKFITNQTK
KDHLTLRKLK*QFKLNNRMWKRFCLKGSICTRK
NQPLSQ*RGS*KI*ALSGRR*TVYFKS*GQSSLT
*LLD*PLLEPLLLRLLLW*HNLWLLRKLPSPN*
KCHLP*CWRYLLWQISTGLGQNLPTGFLCLIKL
*NHRG*WWVTLRISTR*SSSRRQQCRIWNRGVPS
WKNSLPLPKI*KTRPAIKRLEQSLRIELKEFRI
SGMKYKNTFRTGGNS*MKC*RIQHNGWKLRKKL
SRS*DRPEPSLSHGRRVPIQ*MQSKRKSQKPSSW
PKTSASGRQM*MWQMTWP*NFSGIILQMIPEKS
T**QRISMPLGEAFIKG*VSERLLWKKLIDYCN
SSPWTWKSFLPGLQKLKQLPMSYRMLPVRKGS*K
TPRE*KS**NNGKTSKVKLKLTQMFITTWMKTA
KKS*DPWKVPMMQSCYKDVWIT*TSSGVNFGKS
LSTLGPIWKPVLTSGSVCTFLCRNFWCGYS*KMM
N*AGRHLLEATFQQFRSRTMYIGPSRGN*KLKN
L*S*VLLRLYEYF*QSSLWKD*RNSTRSPESCL
LRREPRMSLGFYESRLRRSILSGKN*TCTPLTGR
EK*MRPLKDSGNFKRPRMSWTSSCAKLR*SRDP
GSPWAISSLTLSKITSRKSRHFEEKLRL*KRT*
ATSMTLLASLPLWAFSSHRITSALWKT*TPDGSF
CRWPSRTESGSCMKPTGTLVQHLSTFFPRLSRV
PGREPSRQTKCPTISTTRLKQLAGTIPK*QSST
SL*LT*IMSDSQLIGLP*NSEDCRRPFAWIS*AC
QLHVMPWTSTTSSKMTSPWISCRLLIV*PLFMT
AWSKSTTIWSTSLSAWICV*TGC*MFMIRDEQG
GSVSCLLKLASFPCVKHIWKTSTDTFSSKWQVQQ
DFVTSAGWASFCMILSKFQDSWVKLHPLGAVTL
SQVSGAASNLLIISQRSKRPSS*TG*DWNPSPW
CGCPSCTEWLLQKLPSIRPNVTSAKSVQSLDSGT
GV*STLIMTSAKAAFFLVELQKAIKCTIPWWNI
ALRLHQEKMFETLPRY*KTNFEPKGILRSIPEW
ATCQCRLS*RGTTWKLPLL*STSGQ*ILRLPRPL
SFHTMILIHALNIMLAG*QKWKTAMDLI*MIAS
LLMRA*MMNIC*SSITAKV*TRTPP*ASLVVLP
RS*FP*RVRKEGS*RES*QILRKKTGICKQNMTV
*SSSTNIKACPHCRPLLK*CPPLPRVPGMLSSL
LRPSYCVNTKAAWKPGCKSWKTTINSWSHSYTG
*GSCWSNPRQRPK*MAQRCPLLLPLYRGPTAVSL
CCSEWLAVKLRTPWVRKIFSVLPRTQAQG*RR*
WSNSTTPSLVQEEEIPLESQ*ERTQCRKSFPHG
R*FGQSDGVLSISHDR*RRSRINVLQLLIPAWFL
*YSYNKED*TVRVYKK*IYIFVKGSGIIL*ISV
VSKSVIVLLTMAGFTRLCNCTKKL*ENYM*NLD
S*ITCHFFIWNAFWVV*KFITVIKKDCKLKCAL*
KKVVYKNP*KQNKHTHTHIHTHTQNFEAAHCFA
SFWRDIHMKFMAFSFFAY*R*DFLYHHTK*LLH
TAHLRTVS*VGQA*VFISYIYMSISI*IL*LYR*
RDTNFYRLTFSIF*MFMSHPNRKKLLLVSHPGL
PAWSRMDFSRSRKPGGNYTTLKHCLQLQMFLIL
NNFPLTTKVK*SIGFF*REHVNEYTGLIISE*VI
GWLVD*LID*YIQLPAASNATI*I**CFSGNQS
EGILTL*TSEGIF*LPSSSRTMIGLEGYGFPAH
PCEGVGHSLSEGLDDCS*YIKFSVITTKLLCPLL
TVKRNWVVWFLLLF*IYCPMWDEFLNATRHNLK
*INFGKRCKTVAPSHL*Y*QVSTQKPMNCVSIL
CISLRVVPHRFVSK*ERRQIDSNVTKKPFLVD*T
G*IYKQTNKNCSKKRRKAQEEKLRTGRKKLYSF
MPFYFFLIFKSFIQ*IPPCDL*FCKSVTSDIKC
N*LLESGLTSSVISFWRVGFVHY**LIN*HQTRL
LMLFLPHFGFGVFLIIVHT*VHSFTTCPLRYFL
FPL*FFLFPS*FSKENPKL*GNKLPNYMKIWFL
SCIFFLYVTLDLFFTQGFLKLRFKTRGYFTSY*E
V*VSKFHSKIRGK*SA*IILF*SFCFSFRHISS
GVSLS*YLNKN*ELYCCILSIINLDIISCCVLY
NHQVLNCKS*CN*SINITWHVLSLFSGTEFLLEY
HNILCFNTNTVTFTNYFFKLQFYCIFTTYQTSP
NICLTIVL*YCFTVYLNKARSYV
Frame 2
MRSGGTFDLRKTDCND*DDFANFFSSLFP*CCKEAKVA*TEHPVCPGD*KILTLNPGVPHIH*QAV
GSLYCRQGGRSSNASGSPENPI*FDKS*DQFRR
NEET*SGEGGCPKSPVSD*CCTEKITRCLHEVSI
IPETSQF*AASTRK*DDFR*SEDALACIGNKEC
GTGSSTVTAKSLCELV*KSE*SEV*SGNGDKDW
TSDCTEKADGKSQRT**KSNSFEIAL**AGSKGN
RKKATVGEMLEIVP*DAKGNECLDRMAGSYRYG
IDKEISS*RNA**FGF*SCLGKGYSKRD*ETEG
APEEYHRGRRGLENSFGQEGDVGGR*TQSSE**L
DSCHLPSRRVVKSFVGIPETHGNF*PECGPHHK
VDHSG*HTFG*IREKETPAKRRRA*AFKGRTE*
HTPKGGLYT*PSSKLDGKPR*PLQEISRAPNLRA
QPSICSHFTQN*DWKGLHSFEGIGAV*LRYTKI
A*TTGG*NSAGGESERGRLQ*RYE*RQ*GYCKR
IVAKRRQLTTKNHR*EKARGNKDKTAAVTDKT*C
SQGFEVSKKKKGSRNFSSVVSVQEAG**SPEML
G*H*KKISQPT*AQR*KENKGN*SGIAEEERGA
ECSA*AS*GLV*GWGRNGSGANSDPAQQALAGN*
EQICSVSKTQLCTNSHCP*RNDDGDD*RHAFGN
FLCAFYLFD*NHSCLTSPIRSGTTSQCS*PLC*
GL*RSL*ARGVSEEYKR*STTKLRSD*HYS*QED
SSIAKCNACGKGEATGSSLPA*FPMGKS*QNVQ
GPTRAI*QIC*EMAAFSL*YKDI*SVANRS*TV
SQKDTNS*ELGTC*IQMVS*GTPGWHWAAANCCQ
NIECNWGRNNSAILKNRCQYSTGKIGKPESAVA
GGLQTAVRQKKEARRTKEYLVRISKRFK*ICFM
VGGSR*HC*YPT*TWKRAATKRKA*ASQVTGGRV
APAPGNSQTIK*NWRTRACKCSHKPRRAR*T*K
*AQADKSPVDKGFQSFT*ETRRN*SSNKRPWAA
*KKA*RP*RAVKSSAAVVISY*ESVGNL*PTKPR
RTI*R*GN*NSSSS*TTGCGRDFV*RAAFVQGK
TSHSASEEEVRRSEL*VEGGKPFTSRAEGKAA*
PSSWTDHYWSLSYSDCYSGDTTCGY*GNCHLQTR
NAIFLDVGGTCSGRFQPGLDRTYRLAFSA*SSY
KITEGDGG*P*GYQRDDHQAEGNNAGFGTEASP
VGRTHYRCPKFEKQDQQSRG*NNHYGSN*KNSES
VG*STRTPSEPEATVE*NVKGFNTMAGS*GRS*
AGLRTGQSQA*VMEGGSLYSRCNPKENHRNQAV
GQRPPPVADKCRCGK*LGPETSPGLFCR*YQKSP
HDNREYQCLLEKHS*KGE*ARGCFGRNS*ITAT
VPPGPGKVSCLAYRS*NNCQCPTGCYP*GKAPR
RLQGSKRADETMARPPR*N*SSHRCLSQPG*KQP
KNPEIPGRFR*CSPVTKTFG*HELQVE*TSEKV
SQH*VPFGSQF*PVEASAPFSAGTSGVATAER*
*IKPAGTYWRRLSSSSEAERCT*GLQEGIEN*RT
CNHEYS*DCTNISDRAAFGRTRETLPGAQRAAS
*GESPECHSASTKAG*GGQY*VGKIEPALR*LA
EKNR*DP*KTPGTSRGHG*AGPQAAPS*GDQGIL
AARGRSPH*LSPRSPRESQGTSRRNCASERERE
PRQ*PCSPAYHFGHSALTV*PQHSGRPEHQMEA
SAGGRRGPSQAAA*SPQGLWSSISALSFHVCPGS
LGESHLAKQSALLYQPRDSNNLLGPSQNDRALP
VFS*PE*CQILSL*DCHETPKTAEGPLLGSLEP
VSCM*CLGPAQPQAK*PAHGYPADY*LFDHYL*P
PGARAQQFGQRPSLRGYVSELAAECL*YGTNRE
DPCPVF*NWHHFPV*STFGRQVQIPFQASGKFN
RIL*PAQAGPPSA*FYPNSKTVG*SCILWGQ*H*
AKCPELLPIC***ARDRSGPLPRLDETGTPVHG
VAARPAQSGCCRNCQASGQM*HLQRVSNHWIQV
QESKAL*L*HLPKLLFFWSSCKRP*NALSHGGIL
HSDYIRRRCSRLCQGTKKQISNQKVFCEASPNG
LPASADCLRGGQHGNSRYSDQLLASRFCACLVP
SAFTR*YSFTH*TLC*QASRNGKQQWILSK**HL
S**EHR**TFVNPALLPKFEPGLPPEPAS*SCP
DLDFLRE*GKRGARENPSRS*GRKQESASRI*P
SKAAART*RPVPTAVPS*NDAHLSPESPGC*AHC
*GQATASTQRPPGSQDANPGRPQ*TAGVTVTQA
KAAAGATPGRGQSEWHNGVLSFYLSTEVRQQSA
YAAPSGWQSNFGLHG*GRSSQSSPGHKHRVRGGD
GATQQLLP*FKRKKYPWKANERGHNVGSLFHMA
DDLGRAMESLVSVMTDEEGAE*MFYNS*FPHGF
YNIHTTKRIRQ*EFTRNKSIFL*RVVVLYCRFQ*
FLSLLLFC*QWQVLHVYAIVQKSYKKTTCKILI
AK*LAISLYGTHFGLFKNL*QL*RKIVN*SVLY
KKKLFIKTPKNKTNTHTHTYTHTHKTLRQRIVLH
PFGVISI*NSWLFLFLHIKDKTSSTTTPNDYYT
LLI*ELSAEWGRLEFSFHISICL*VYKYYSYID
KEIRISID*LFPFFKCSCHILIERNYF*SVIQAY
LLGLEWIFPGAGSQEETTPH*NIVYSSRCFSF*
TTFH*QRK*SKVLDFFKGNM*MNTQDLLYQSE*
SVGWLID*LIDTFSFLLLAMPRFRFNDASVEINQ
KVF*PCEHQKVFFNSQAVAGR**GWRAMDSQPI
PVKE*ATL*VKDWMIVHNT*SSL*LQLNYYALF
SQSKGTGWFGFCCFFRFIVPCGMSF*MPQDII*N
K*TLGKGVKQ*PHHICDTDRYQPRSP*TVFPSF
AFLCE*FHTGL*VSKKEGKLIQMLQKNPSWWIR
QVKYINKQTKIAQKRGEKLKRKS*GLVGKSFTLS
CHFISF*FLNHSFNRYHRVTYNFANLLPLTSSV
ISFWRVG*HQV*LAFGEWVLSIINN*LINIKHG
FSCYFYLTLVLGCS**LCTPEFTASPLVHCVIFF
FLYNSFFFLHNFQKKTQSSKVTNYQIT*RFGFC
LAFFSFM*RWTFSLPKDF*NSDLKQGVTLHPTK
KFK*VSFILKSEVNRVHK*FCFNLFVFLLDTLAL
E*VCHNI*TKIESFIAAF*A*LIWTLFRVVFFI
TTKY*TVNHNVTEA*TSHGMFCHCFQVLSSYLS
IIIYCVLTPTL*HLRIIFLNFSFTAFSQHIRLHQ
IYALLLYYSTALLCISIKHAVML
Sequence NM_004009
ATGCTGTCTGTGAAGCTGAATCTGTGAGAACACCTCACTATTCACGGCAACCGGAGTGGAAGAAACAGGTGCAAAAAGATTGTGTGTTTGTCTGCTTTTGTGAGGCTGGTCAGAGATTCTGTGCCTGCTTTATCTGTGCTTGGCTATGACTCTACCTCCAGGTTTACCATACCCCATAGAATGTGTAAGAGAAAAGTACC
AACAGGGAAATCAGCAAAAAGCTTTCCTATGAAGGTGTGTAGCCAGCCTCCGCAGAATTTGAAATGTCTGAGGTCTCATCTGATGAAAGAGAAGATGTTC
AAAAGAAAACATTCACAAAATGGGTAAATGCACAATTTTCTAAGTTTGGGAAGCAGCATATTGAGAACCTCTTCAGTGACCTACAGGATGGGAGGCGCCT
CCTAGACCTCCTCGAAGGCCTGACAGGGCAAAAACTGCCAAAAGAAAAAGGATCCACAAGAGTTCATGCCCTGAACAATGTCAACAAGGCACTGCGGGTT
TTGCAGAACAATAATGTTGATTTAGTGAATATTGGAAGTACTGACATCGTAGATGGAAATCATAAACTGACTCTTGGTTTGATTTGGAATATAATCCTCC
ACTGGCAGGTCAAAAATGTAATGAAAAATATCATGGCTGGATTGCAACAAACCAACAGTGAAAAGATTCTCCTGAGCTGGGTCCGACAATCAACTCGTAA
TTATCCACAGGTTAATGTAATCAACTTCACCACCAGCTGGTCTGATGGCCTGGCTTTGAATGCTCTCATCCATAGTCATAGGCCAGACCTATTTGACTGG
AATAGTGTGGTTTGCCAGCAGTCAGCCACACAACGACTGGAACATGCATTCAACATCGCCAGATATCAATTAGGCATAGAGAAACTACTCGATCCTGAAG
ATGTTGATACCACCTATCCAGATAAGAAGTCCATCTTAATGTACATCACATCACTCTTCCAAGTTTTGCCTCAACAAGTGAGCATTGAAGCCATCCAGGA
AGTGGAAATGTTGCCAAGGCCACCTAAAGTGACTAAAGAAGAACATTTTCAGTTACATCATCAAATGCACTATTCTCAACAGATCACGGTCAGTCTAGCA
CAGGGATATGAGAGAACTTCTTCCCCTAAGCCTCGATTCAAGAGCTATGCCTACACACAGGCTGCTTATGTCACCACCTCTGACCCTACACGGAGCCCAT
TTCCTTCACAGCATTTGGAAGCTCCTGAAGACAAGTCATTTGGCAGTTCATTGATGGAGAGTGAAGTAAACCTGGACCGTTATCAAACAGCTTTAGAAGA
AGTATTATCGTGGCTTCTTTCTGCTGAGGACACATTGCAAGCACAAGGAGAGATTTCTAATGATGTGGAAGTGGTGAAAGACCAGTTTCATACTCATGAG
GGGTACATGATGGATTTGACAGCCCATCAGGGCCGGGTTGGTAATATTCTACAATTGGGAAGTAAGCTGATTGGAACAGGAAAATTATCAGAAGATGAAG
AAACTGAAGTACAAGAGCAGATGAATCTCCTAAATTCAAGATGGGAATGCCTCAGGGTAGCTAGCATGGAAAAACAAAGCAATTTACATAGAGTTTTAAT
GGATCTCCAGAATCAGAAACTGAAAGAGTTGAATGACTGGCTAACAAAAACAGAAGAAAGAACAAGGAAAATGGAGGAAGAGCCTCTTGGACCTGATCTT
GAAGACCTAAAACGCCAAGTACAACAACATAAGGTGCTTCAAGAAGATCTAGAACAAGAACAAGTCAGGGTCAATTCTCTCACTCACATGGTGGTGGTAG
TTGATGAATCTAGTGGAGATCACGCAACTGCTGCTTTGGAAGAACAACTTAAGGTATTGGGAGATCGATGGGCAAACATCTGTAGATGGACAGAAGACCG
CTGGGTTCTTTTACAAGACATCCTTCTCAAATGGCAACGTCTTACTGAAGAACAGTGCCTTTTTAGTGCATGGCTTTCAGAAAAAGAAGATGCAGTGAAC
AAGATTCACACAACTGGCTTTAAAGATCAAAATGAAATGTTATCAAGTCTTCAAAAACTGGCCGTTTTAAAAGCGGATCTAGAAAAGAAAAAGCAATCCA
TGGGCAAACTGTATTCACTCAAACAAGATCTTCTTTCAACACTGAAGAATAAGTCAGTGACCCAGAAGACGGAAGCATGGCTGGATAACTTTGCCCGGTG
TTGGGATAATTTAGTCCAAAAACTTGAAAAGAGTACAGCACAGATTTCACAGGCTGTCACCACCACTCAGCCATCACTAACACAGACAACTGTAATGGAA
ACAGTAACTACGGTGACCACAAGGGAACAGATCCTGGTAAAGCATGCTCAAGAGGAACTTCCACCACCACCTCCCCAAAAGAAGAGGCAGATTACTGTGG
ATTCTGAAATTAGGAAAAGGTTGGATGTTGATATAACTGAACTTCACAGCTGGATTACTCGCTCAGAAGCTGTGTTGCAGAGTCCTGAATTTGCAATCTT
TCGGAAGGAAGGCAACTTCTCAGACTTAAAAGAAAAAGTCAATGCCATAGAGCGAGAAAAAGCTGAGAAGTTCAGAAAACTGCAAGATGCCAGCAGATCA
GCTCAGGCCCTGGTGGAACAGATGGTGAATGAGGGTGTTAATGCAGATAGCATCAAACAAGCCTCAGAACAACTGAACAGCCGGTGGATCGAATTCTGCC
AGTTGCTAAGTGAGAGACTTAACTGGCTGGAGTATCAGAACAACATCATCGCTTTCTATAATCAGCTACAACAATTGGAGCAGATGACAACTACTGCTGA
AAACTGGTTGAAAATCCAACCCACCACCCCATCAGAGCCAACAGCAATTAAAAGTCAGTTAAAAATTTGTAAGGATGAAGTCAACCGGCTATCAGATCTT
CAACCTCAAATTGAACGATTAAAAATTCAAAGCATAGCCCTGAAAGAGAAAGGACAAGGACCCATGTTCCTGGATGCAGACTTTGTGGCCTTTACAAATC
ATTTTAAGCAAGTCTTTTCTGATGTGCAGGCCAGAGAGAAAGAGCTACAGACAATTTTTGACACTTTGCCACCAATGCGCTATCAGGAGACCATGAGTGC
CATCAGGACATGGGTCCAGCAGTCAGAAACCAAACTCTCCATACCTCAACTTAGTGTCACCGACTATGAAATCATGGAGCAGAGACTCGGGGAATTGCAG
GCTTTACAAAGTTCTCTGCAAGAGCAACAAAGTGGCCTATACTATCTCAGCACCACTGTGAAAGAGATGTCGAAGAAAGCGCCCTCTGAAATTAGCCGGA
AATATCAATCAGAATTTGAAGAAATTGAGGGACGCTGGAAGAAGCTCTCCTCCCAGCTGGTTGAGCATTGTCAAAAGCTAGAGGAGCAAATGAATAAACT
CCGAAAAATTCAGAATCACATACAAACCCTGAAGAAATGGATGGCTGAAGTTGATGTTTTTCTGAAGGAGGAATGGCCTGCCCTTGGGGATTCAGAAATT
CTAAAAAAGCAGCTGAAACAGTGCAGACTTTTAGTCAGTGATATTCAGACAATTCAGCCCAGTCTAAACAGTGTCAATGAAGGTGGGCAGAAGATAAAGA
ATGAAGCAGAGCCAGAGTTTGCTTCGAGACTTGAGACAGAACTCAAAGAACTTAACACTCAGTGGGATCACATGTGCCAACAGGTCTATGCCAGAAAGGA
GGCCTTGAAGGGAGGTTTGGAGAAAACTGTAAGCCTCCAGAAAGATCTATCAGAGATGCACGAATGGATGACACAAGCTGAAGAAGAGTATCTTGAGAGA
GATTTTGAATATAAAACTCCAGATGAATTACAGAAAGCAGTTGAAGAGATGAAGAGAGCTAAAGAAGAGGCCCAACAAAAAGAAGCGAAAGTGAAACTCC
TTACTGAGTCTGTAAATAGTGTCATAGCTCAAGCTCCACCTGTAGCACAAGAGGCCTTAAAAAAGGAACTTGAAACTCTAACCACCAACTACCAGTGGCT
CTGCACTAGGCTGAATGGGAAATGCAAGACTTTGGAAGAAGTTTGGGCATGTTGGCATGAGTTATTGTCATACTTGGAGAAAGCAAACAAGTGGCTAAAT
GAAGTAGAATTTAAACTTAAAACCACTGAAAACATTCCTGGCGGAGCTGAGGAAATCTCTGAGGTGCTAGATTCACTTGAAAATTTGATGCGACATTCAG
AGGATAACCCAAATCAGATTCGCATATTGGCACAGACCCTAACAGATGGCGGAGTCATGGATGAGCTAATCAATGAGGAACTTGAGACATTTAATTCTCG
TTGGAGGGAACTACATGAAGAGGCTGTAAGGAGGCAAAAGTTGCTTGAACAGAGCATCCAGTCTGCCCAGGAGACTGAAAAATCCTTACACTTAATCCAG
GAGTCCCTCACATTCATTGACAAGCAGTTGGCAGCTTATATTGCAGACAAGGTGGACGCAGCTCAAATGCCTCAGGAAGCCCAGAAAATCCAATCTGATT
TGACAAGTCATGAGATCAGTTTAGAAGAAATGAAGAAACATAATCAGGGGAAGGAGGCTGCCCAAAGAGTCCTGTCTCAGATTGATGTTGCACAGAAAAA
ATTACAAGATGTCTCCATGAAGTTTCGATTATTCCAGAAACCAGCCAATTTTGAGCAGCGTCTACAAGAAAGTAAGATGATTTTAGATGAAGTGAAGATG
CACTTGCCTGCATTGGAAACAAAGAGTGTGGAACAGGAAGTAGTACAGTCACAGCTAAATCATTGTGTGAACTTGTATAAAAGTCTGAGTGAAGTGAAGT
CTGAAGTGGAAATGGTGATAAAGACTGGACGTCAGATTGTACAGAAAAAGCAGACGGAAAATCCCAAAGAACTTGATGAAAGAGTAACAGCTTTGAAATT
GCATTATAATGAGCTGGGAGCAAAGGTAACAGAAAGAAAGCAACAGTTGGAGAAATGCTTGAAATTGTCCCGTAAGATGCGAAAGGAAATGAATGTCTTG
ACAGAATGGCTGGCAGCTACAGATATGGAATTGACAAAGAGATCAGCAGTTGAAGGAATGCCTAGTAATTTGGATTCTGAAGTTGCCTGGGGAAAGGCTA
CTCAAAAAGAGATTGAGAAACAGAAGGTGCACCTGAAGAGTATCACAGAGGTAGGAGAGGCCTTGAAAACAGTTTTGGGCAAGAAGGAGACGTTGGTGGA
AGATAAACTCAGTCTTCTGAATAGTAACTGGATAGCTGTCACCTCCCGAGCAGAAGAGTGGTTAAATCTTTTGTTGGAATACCAGAAACACATGGAAACT
TTTGACCAGAATGTGGACCACATCACAAAGTGGATCATTCAGGCTGACACACTTTTGGATGAATCAGAGAAAAAGAAACCCCAGCAAAAAGAAGACGTGC
TTAAGCGTTTAAAGGCAGAACTGAATGACATACGCCCAAAGGTGGACTCTACACGTGACCAAGCAGCAAACTTGATGGCAAACCGCGGTGACCACTGCAG
GAAATTAGTAGAGCCCCAAATCTCAGAGCTCAACCATCGATTTGCAGCCATTTCACACAGAATTAAGACTGGAAAGGCCTCCATTCCTTTGAAGGAATTG
GAGCAGTTTAACTCAGATATACAAAAATTGCTTGAACCACTGGAGGCTGAAATTCAGCAGGGGGTGAATCTGAAAGAGGAAGACTTCAATAAAGATATGA
ATGAAGACAATGAGGGTACTGTAAAAGAATTGTTGCAAAGAGGAGACAACTTACAACAAAGAATCACAGATGAGAGAAAGCGAGAGGAAATAAAGATAAA
ACAGCAGCTGTTACAGACAAAACATAATGCTCTCAAGGATTTGAGGTCTCAAAGAAGAAAAAAGGCTCTAGAAATTTCTCATCAGTGGTATCAGTACAAG
AGGCAGGCTGATGATCTCCTGAAATGCTTGGATGACATTGAAAAAAAATTAGCCAGCCTACCTGAGCCCAGAGATGAAAGGAAAATAAAGGAAATTGATC
GGGAATTGCAGAAGAAGAAAGAGGAGCTGAATGCAGTGCGTAGGCAAGCTGAGGGCTTGTCTGAGGATGGGGCCGCAATGGCAGTGGAGCCAACTCAGAT
CCAGCTCAGCAAGCGCTGGCGGGAAATTGAGAGCAAATTTGCTCAGTTTCGAAGACTCAACTTTGCACAAATTCACACTGTCCGTGAAGAAACGATGATG
GTGATGACTGAAGACATGCCTTTGGAAATTTCTTATGTGCCTTCTACTTATTTGACTGAAATCACTCATGTCTCACAAGCCCTATTAGAAGTGGAACAAC
TTCTCAATGCTCCTGACCTCTGTGCTAAGGACTTTGAAGATCTCTTTAAGCAAGAGGAGTCTCTGAAGAATATAAAAGATAGTCTACAACAAAGCTCAGG
TCGGATTGACATTATTCATAGCAAGAAGACAGCAGCATTGCAAAGTGCAACGCCTGTGGAAAGGGTGAAGCTACAGGAAGCTCTCTCCCAGCTTGATTTC
CAATGGGAAAAAGTTAACAAAATGTACAAGGACCGACAAGGGCGATTTGACAGATCTGTTGAGAAATGGCGGCGTTTTCATTATGATATAAAGATATTTA
ATCAGTGGCTAACAGAAGCTGAACAGTTTCTCAGAAAGACACAAATTCCTGAGAATTGGGAACATGCTAAATACAAATGGTATCTTAAGGAACTCCAGGA
TGGCATTGGGCAGCGGCAAACTGTTGTCAGAACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGATGCCAGTATTCTACAGGAA
AAATTGGGAAGCCTGAATCTGCGGTGGCAGGAGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGGCTAGAAGAACAAAAGAATATCTTGTCAGAATTTC
AAAGAGATTTAAATGAATTTGTTTTATGGTTGGAGGAAGCAGATAACATTGCTAGTATCCCACTTGAACCTGGAAAAGAGCAGCAACTAAAAGAAAAGCT
TGAGCAAGTCAAGTTACTGGTGGAAGAGTTGCCCCTGCGCCAGGGAATTCTCAAACAATTAAATGAAACTGGAGGACCCGTGCTTGTAAGTGCTCCCATA
AGCCCAGAAGAGCAAGATAAACTTGAAAATAAGCTCAAGCAGACAAATCTCCAGTGGATAAAGGTTTCCAGAGCTTTACCTGAGAAACAAGGAGAAATTG
AAGCTCAAATAAAAGACCTTGGGCAGCTTGAAAAAAAGCTTGAAGACCTTGAAGAGCAGTTAAATCATCTGCTGCTGTGGTTATCTCCTATTAGGAATCA
GTTGGAAATTTATAACCAACCAAACCAAGAAGGACCATTTGACGTTAAGGAAACTGAAATAGCAGTTCAAGCTAAACAACCGGATGTGGAAGAGATTTTG
TCTAAAGGGCAGCATTTGTACAAGGAAAAACCAGCCACTCAGCCAGTGAAGAGGAAGTTAGAAGATCTGAGCTCTGAGTGGAAGGCGGTAAACCGTTTAC
TTCAAGAGCTGAGGGCAAAGCAGCCTGACCTAGCTCCTGGACTGACCACTATTGGAGCCTCTCCTACTCAGACTGTTACTCTGGTGACACAACCTGTGGT
TACTAAGGAAACTGCCATCTCCAAACTAGAAATGCCATCTTCCTTGATGTTGGAGGTACCTGCTCTGGCAGATTTCAACCGGGCTTGGACAGAACTTACC
GACTGGCTTTCTCTGCTTGATCAAGTTATAAAATCACAGAGGGTGATGGTGGGTGACCTTGAGGATATCAACGAGATGATCATCAAGCAGAAGGCAACAA
TGCAGGATTTGGAACAGAGGCGTCCCCAGTTGGAAGAACTCATTACCGCTGCCCAAAATTTGAAAAACAAGACCAGCAATCAAGAGGCTAGAACAATCAT
TACGGATCGAATTGAAAGAATTCAGAATCAGTGGGATGAAGTACAAGAACACCTTCAGAACCGGAGGCAACAGTTGAATGAAATGTTAAAGGATTCAACA
CAATGGCTGGAAGCTAAGGAAGAAGCTGAGCAGGTCTTAGGACAGGCCAGAGCCAAGCTTGAGTCATGGAAGGAGGGTCCCTATACAGTAGATGCAATCC
AAAAGAAAATCACAGAAACCAAGCAGTTGGCCAAAGACCTCCGCCAGTGGCAGACAAATGTAGATGTGGCAAATGACTTGGCCCTGAAACTTCTCCGGGA
TTATTCTGCAGATGATACCAGAAAAGTCCACATGATAACAGAGAATATCAATGCCTCTTGGAGAAGCATTCATAAAAGGGTGAGTGAGCGAGAGGCTGCT
TTGGAAGAAACTCATAGATTACTGCAACAGTTCCCCCTGGACCTGGAAAAGTTTCTTGCCTGGCTTACAGAAGCTGAAACAACTGCCAATGTCCTACAGG
ATGCTACCCGTAAGGAAAGGCTCCTAGAAGACTCCAAGGGAGTAAAAGAGCTGATGAAACAATGGCAAGACCTCCAAGGTGAAATTGAAGCTCACACAGA
TGTTTATCACAACCTGGATGAAAACAGCCAAAAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTACAAAGACGTTTGGATAACATGAAC
TTCAAGTGGAGTGAACTTCGGAAAAAGTCTCTCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCGTCTGCACCTTTCTCTGCAGGAAC
TTCTGGTGTGGCTACAGCTGAAAGATGATGAATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAGAAGCAGAACGATGTACATAGGGC
CTTCAAGAGGGAATTGAAAACTAAAGAACCTGTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGCAGCCTTTGGAAGGACTAGAGAAA
CTCTACCAGGAGCCCAGAGAGCTGCCTCCTGAGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGAGGAGGTCAATACTGAGTGGGAAA
AATTGAACCTGCACTCCGCTGACTGGCAGAGAAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCCACGGATGAGCTGGACCTCAAGCT
GCGCCAAGCTGAGGTGATCAAGGGATCCTGGCAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCGAGAAAGTCAAGGCACTTCGAGGA
GAAATTGCGCCTCTGAAAGAGAACGTGAGCCACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCTCTCACCGTATAACCTCAGCACTC
TGGAAGACCTGAACACCAGATGGAAGCTTCTGCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCACAGGGACTTTGGTCCAGCATCTCA
GCACTTTCTTTCCACGTCTGTCCAGGGTCCCTGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACCACGAGACTCAAACAACTTGCTGG
GACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGCCATGAAACTCCGAAGACTGCAGA
AGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGACCAGCCCATGGATATCCTGCAGAT
TATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGGATATGTGTCTGAACTGGCTGCTG
AATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGCACATTTGGAAGACAAGTACAGAT
ACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATCCAAATTCCAAGACAGTTGGGTGA
AGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGATCGAAGCGGCCCTCTTCCTAGAC
TGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCATCAGGCCAAATGTAACATCTGCA
AAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGTCGAGTTGCAAAAGGCCA
TAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTAAAAAACAAATTTCGAACCAAA
AGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCCCGTTACTCTGATCAACTTCTGGC
CAGTAGATTCTGCGCCTGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCAGAAATGGAAAACAG
CAATGGATCTTATCTAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTTTGAACCAGGACTCC
CCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAA
ACAGGAATCTGCAAGCAGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATGATGCCCACCTCTCC
CCAGAGTCCCCGGGATGCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCCTGGAAGACCACAAT
AAACAGCTGGAGTCACAGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTCCTCTCCTTCTACCT
CTCTACAGAGGTCCGACAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTTCTCAGTCCTCCCCA
GGACACAAGCACAGGGTTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCCCTGGAAAGCCAATGAGAGAGGAC
ACAATGTAGGAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGAGCAGAATAAATGTT
TTACAACTCCTGATTCCCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTATATTTTTGTGAAGG
GTAGTGGTATTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTT
ATAAGAAAACTACATGTAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAG
AAAGATTGTAAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACATACACACACACACAC
AAAACTTTGAGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAAAGATAAGACTTCCT
CTACCACCACACCAAATGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATA
AGTATATAAATACTATAGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGA
AATTACTTCTAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACCACACTAAAACATTG
TCTACAGCTCCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAACATGTGAATGAATAC
ACAGGACTTATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGA
TTTAATGATGCTTCAGTGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGG
GCTGGAGGGCTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACATAAAGTTCTCTGTA
ATTACAACTAAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCCCATGTGGGATGAGT
TTTTAAATGCCACAAGACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGGTATCAACCCA
GAAGCCCATGAACTGTGTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTT
ACAAAAAAACCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCAAGAGGAAAAGCTAA
GGACTGGTAGGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACCGTGTGACCTATAATTTTG
CAAATCTGTTACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAA
TAATTAATTAATTAACATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCT
TCACCACTTGTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTCTAAGGTAACAAATT
ACCAAATTACATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAAAACTCAGATTTAAA
ACAAGGGGTTACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTT
TTTGTTTTTCTTTTAGACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTT
GGACATTATTTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGCATGTTTTGTCATTG
TTTTCAGGTACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTTTTAAACTTCAGTTTTACT
GCATTTTCACAACATATCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
MLSVKLNL*EHLTIHGNRSGRNRCKKIVCLSAFVRLVRDSVPALSVLGYDSTSRFTIPHRMCKRKVP
TGKSAKSFPMKVCSQPPQNLKCLRSHLMKEKMF
KRKHSQNG*MHNFLSLGSSILRTSSVTYRMGGA
S*TSSKA*QGKNCQKKKDPQEFMP*TMSTRHCGF
CRTIMLI**ILEVLTS*MEIIN*LLV*FGI*SS
TGRSKM**KISWLDCNKPTVKRFS*AGSDNQLV
IIHRLM*STSPPAGLMAWL*MLSSIVIGQTYLTG
IVWFASSQPHNDWNMHSTSPDIN*A*RNYSILK
MLIPPIQIRSPS*CTSHHSSKFCLNK*ALKPSR
KWKCCQGHLK*LKKNIFSYIIKCTILNRSRSV*H
RDMRELLPLSLDSRAMPTHRLLMSPPLTLHGAH
FLHSIWKLLKTSHLAVH*WRVK*TWTVIKQL*K
KYYRGFFLLRTHCKHKERFLMMWKW*KTSFILMR
GT*WI*QPIRAGLVIFYNWEVS*LEQENYQKMK
KLKYKSR*IS*IQDGNASG*LAWKNKAIYIEF*
WISRIRN*KS*MTG*QKQKKEQGKWRKSLLDLIL
KT*NAKYNNIRCFKKI*NKNKSGSILSLTWWW*
LMNLVEITQLLLWKNNLRYWEIDGQTSVDGQKT
AGFFYKTSFSNGNVLLKNSAFLVHGFQKKKMQ*T
RFTQLALKIKMKCYQVFKNWPF*KRI*KRKSNP
WANCIHSNKIFFQH*RISQ*PRRRKHGWITLPG
VGII*SKNLKRVQHRFHRLSPPLSHH*HRQL*WK
Q*LR*PQGNRSW*SMLKRNFHHHLPKRRGRLLW
ILKLGKGWMLI*LNFTAGLLAQKLCCRVLNLQS
FGRKATSQT*KKKSMP*SEKKLRSSENCKMPADQ
LRPWWNRW*MRVLMQIASNKPQNN*TAGGSNSA
SC*VRDLTGWSIRTTSSLSIISYNNWSR*QLLL
KTG*KSNPPPHQSQQQLKVS*KFVRMKSTGYQIF
NLKLND*KFKA*P*KRKDKDPCSWMQTLWPLQI
ILSKSFLMCRPERKSYRQFLTLCHQCAIRRP*V
PSGHGSSSQKPNSPYLNLVSPTMKSWSRDSGNCR
LYKVLCKSNKVAYTISAPL*KRCRRKRPLKLAG
NINQNLKKLRDAGRSSPPSWLSIVKS*RSK*IN
SEKFRITYKP*RNGWLKLMFF*RRNGLPLGIQKF
*KSS*NSADF*SVIFRQFSPV*TVSMKVGRR*R
MKQSQSLLRDLRQNSKNLTLSGITCANRSMPER
RP*REVWRKL*ASRKIYQRCTNG*HKLKKSILRE
ILNIKLQMNYRKQLKR*RELKKRPNKKKRK*NS
LLSL*IVS*LKLHL*HKRP*KRNLKL*PPTTSG
SALG*MGNARLWKKFGHVGMSYCHTWRKQTSG*M
K*NLNLKPLKTFLAELRKSLRC*IHLKI*CDIQ
RITQIRFAYWHRP*QMAESWMS*SMRNLRHLIL
VGGNYMKRL*GGKSCLNRASSLPRRLKNPYT*SR
SPSHSLTSSWQLILQTRWTQLKCLRKPRKSNLI
*QVMRSV*KK*RNIIRGRRLPKESCLRLMLHRK
NYKMSP*SFDYSRNQPILSSVYKKVR*F*MK*RC
TCLHWKQRVWNRK*YSHS*IIV*TCIKV*VK*S
LKWKW**RLDVRLYRKSRRKIPKNLMKE*QL*N
CIIMSWEQR*QKESNSWRNA*NCPVRCERK*MS*
QNGWQLQIWN*QRDQQLKECLVIWILKLPGERL
LKKRLRNRRCT*RVSQR*ERP*KQFWARRRRWW
KINSVF*IVTG*LSPPEQKSG*IFCWNTRNTWKL
LTRMWTTSQSGSFRLTHFWMNQRKRNPSKKKTC
LSV*RQN*MTYAQRWTLHVTKQQT*WQTAVTTA
GN**SPKSQSSTIDLQPFHTELRLERPPFL*RNW
SSLTQIYKNCLNHWRLKFSRG*I*KRKTSIKI*
MKTMRVL*KNCCKEETTYNKESQMRESERK*R*
NSSCYRQNIMLSRI*GLKEEKRL*KFLISGISTR
GRLMIS*NAWMTLKKN*PAYLSPEMKGK*RKLI
GNCRRRKRS*MQCVGKLRACLRMGPQWQWSQLR
SSSASAGGKLRANLLSFEDSTLHKFTLSVKKR*W
**LKTCLWKFLMCLLLI*LKSLMSHKPY*KWNN
FSMLLTSVLRTLKISLSKRSL*RI*KIVYNKAQ
VGLTLFIARRQQHCKVQRLWKG*SYRKLSPSLIS
NGKKLTKCTRTDKGDLTDLLRNGGVFIMI*RYL
ISG*QKLNSFSERHKFLRIGNMLNTNGILRNSR
MALGSGKLLSEH*MQLGKK*FSNPQKQMPVFYRK
NWEA*ICGGRRSANSCQTEKRG*KNKRISCQNF
KEI*MNLFYGWRKQITLLVSHLNLEKSSN*KKS
LSKSSYWWKSCPCAREFSNN*MKLEDPCL*VLP*
AQKSKINLKISSSRQISSG*RFPELYLRNKEKL
KLK*KTLGSLKKSLKTLKSS*IICCCGYLLLGI
SWKFITNQTKKDHLTLRKLK*QFKLNNRMWKRFC
LKGSICTRKNQPLSQ*RGS*KI*ALSGRR*TVY
FKS*GQSSLT*LLD*PLLEPLLLRLLLW*HNLW
LLRKLPSPN*KCHLP*CWRYLLWQISTGLGQNLP
TGFLCLIKL*NHRG*WWVTLRISTR*SSSRRQQ
CRIWNRGVPSWKNSLPLPKI*KTRPAIKRLEQS
LRIELKEFRISGMKYKNTFRTGGNS*MKC*RIQH
NGWKLRKKLSRS*DRPEPSLSHGRRVPIQ*MQS
KRKSQKPSSWPKTSASGRQM*MWQMTWP*NFSG
IILQMIPEKST**QRISMPLGEAFIKG*VSERLL
WKKLIDYCNSSPWTWKSFLPGLQKLKQLPMSYR
MLPVRKGS*KTPRE*KS**NNGKTSKVKLKLTQ
MFITTWMKTAKKS*DPWKVPMMQSCYKDVWIT*T
SSGVNFGKSLSTLGPIWKPVLTSGSVCTFLCRN
FWCGYS*KMMN*AGRHLLEATFQQFRSRTMYIG
PSRGN*KLKNL*S*VLLRLYEYF*QSSLWKD*RN
STRSPESCLLRREPRMSLGFYESRLRRSILSGK
N*TCTPLTGREK*MRPLKDSGNFKRPRMSWTSS
CAKLR*SRDPGSPWAISSLTLSKITSRKSRHFEE
KLRL*KRT*ATSMTLLASLPLWAFSSHRITSAL
WKT*TPDGSFCRWPSRTESGSCMKPTGTLVQHL
STFFPRLSRVPGREPSRQTKCPTISTTRLKQLAG
TIPK*QSSTSL*LT*IMSDSQLIGLP*NSEDCR
RPFAWIS*ACQLHVMPWTSTTSSKMTSPWISCR
LLIV*PLFMTAWSKSTTIWSTSLSAWICV*TGC*
MFMIRDEQGGSVSCLLKLASFPCVKHIWKTSTD
TFSSKWQVQQDFVTSAGWASFCMILSKFQDSWV
KLHPLGAVTLSQVSGAASNLLIISQRSKRPSS*T
G*DWNPSPWCGCPSCTEWLLQKLPSIRPNVTSA
KSVQSLDSGTGV*STLIMTSAKAAFFLVELQKA
IKCTIPWWNIALRLHQEKMFETLPRY*KTNFEPK
GILRSIPEWATCQCRLS*RGTTWKLPLL*STSG
Q*ILRLPRPLSFHTMILIHALNIMLAG*QKWKT
AMDLI*MIASLLMRA*MMNIC*SSITAKV*TRTP
P*ASLVVLPRS*FP*RVRKEGS*RES*QILRKK
TGICKQNMTV*SSSTNIKACPHCRPLLK*CPPL
PRVPGMLSSLLRPSYCVNTKAAWKPGCKSWKTTI
NSWSHSYTG*GSCWSNPRQRPK*MAQRCPLLLP
LYRGPTAVSLCCSEWLAVKLRTPWVRKIFSVLP
RTQAQG*RR*WSNSTTPSLVQEEEIPLESQ*ERT
QCRKSFPHGR*FGQSDGVLSISHDR*RRSRINV
LQLLIPAWFL*YSYNKED*TVRVYKK*IYIFVK
GSGIIL*ISVVSKSVIVLLTMAGFTRLCNCTKKL
*ENYM*NLDS*ITCHFFIWNAFWVV*KFITVIK
KDCKLKCAL*KKVVYKNP*KQNKHTHTHIHTHT
QNFEAAHCFASFWRDIHMKFMAFSFFAY*R*DFL
YHHTK*LLHTAHLRTVS*VGQA*VFISYIYMSI
SI*IL*LYR*RDTNFYRLTFSIF*MFMSHPNRK
KLLLVSHPGLPAWSRMDFSRSRKPGGNYTTLKHC
LQLQMFLILNNFPLTTKVK*SIGFF*REHVNEY
TGLIISE*VIGWLVD*LID*YIQLPAASNATI*
I**CFSGNQSEGILTL*TSEGIF*LPSSSRTMIG
LEGYGFPAHPCEGVGHSLSEGLDDCS*YIKFSV
ITTKLLCPLLTVKRNWVVWFLLLF*IYCPMWDE
FLNATRHNLK*INFGKRCKTVAPSHL*Y*QVSTQ
KPMNCVSILCISLRVVPHRFVSK*ERRQIDSNV
TKKPFLVD*TG*IYKQTNKNCSKKRRKAQEEKL
RTGRKKLYSFMPFYFFLIFKSFIQ*IPPCDL*FC
KSVTSDIKCN*LLESGLTSSVISFWRVGFVHY*
*LIN*HQTRLLMLFLPHFGFGVFLIIVHT*VHS
FTTCPLRYFLFPL*FFLFPS*FSKENPKL*GNKL
PNYMKIWFLSCIFFLYVTLDLFFTQGFLKLRFK
TRGYFTSY*EV*VSKFHSKIRGK*SA*IILF*S
FCFSFRHISSGVSLS*YLNKN*ELYCCILSIINL
DIISCCVLYNHQVLNCKS*CN*SINITWHVLSL
FSGTEFLLEYHNILCFNTNTVTFTNYFFKLQFY
CIFTTYQTSPNICLTIVL*YCFTVYLNKARSYV
Frame 1
CCL*S*ICENTSLFTATGVEETGAKRLCVCLLL*GWSEILCLLYLCLAMTLPPGLPYPIECVREKY
QQGNQQKAFL*RCVASLRRI*NV*GLI**KRRCS
KENIHKMGKCTIF*VWEAAY*EPLQ*PTGWEAP
PRPPRRPDRAKTAKRKRIHKSSCPEQCQQGTAG
FAEQ*C*FSEYWKY*HRRWKS*TDSWFDLEYNPP
LAGQKCNEKYHGWIATNQQ*KDSPELGPTINS*
LSTG*CNQLHHQLV*WPGFECSHP*S*ARPI*L
E*CGLPAVSHTTTGTCIQHRQISIRHRETTRS*R
C*YHLSR*EVHLNVHHITLPSFASTSEH*SHPG
SGNVAKAT*SD*RRTFSVTSSNALFSTDHGQSS
TGI*ENFFP*ASIQELCLHTGCLCHHL*PYTEPI
SFTAFGSS*RQVIWQFIDGE*SKPGPLSNSFRR
SIIVASFC*GHIASTRRDF**CGSGERPVSYS*
GVHDGFDSPSGPGW*YSTIGK*ADWNRKIIRR*R
N*STRADESPKFKMGMPQGS*HGKTKQFT*SFN
GSPESETERVE*LANKNRRKNKENGGRASWT*S
*RPKTPSTTT*GASRRSRTRTSQGQFSHSHGGGS
**I*WRSRNCCFGRTT*GIGRSMGKHL*MDRRP
LGSFTRHPSQMATSY*RTVPF*CMAFRKRRCSE
QDSHNWL*RSK*NVIKSSKTGRFKSGSRKEKAIH
GQTVFTQTRSSFNTEE*VSDPEDGSMAG*LCPV
LG*FSPKT*KEYSTDFTGCHHHSAITNTDNCNG
NSNYGDHKGTDPGKACSRGTSTTTSPKEEADYCG
F*N*EKVGC*YN*TSQLDYSLRSCVAES*ICNL
SEGRQLLRLKRKSQCHRARKS*EVQKTARCQQI
SSGPGGTDGE*GC*CR*HQTSLRTTEQPVDRILP
VAK*ET*LAGVSEQHHRFL*SATTIGADDNYC*
KLVENPTHHPIRANSN*KSVKNL*G*SQPAIRS
STSN*TIKNSKHSPERERTRTHVPGCRLCGLYKS
F*ASLF*CAGQRERATDNF*HFATNALSGDHEC
HQDMGPAVRNQTLHTST*CHRL*NHGAETRGIA
GFTKFSARATKWPILSQHHCERDVEESAL*N*PE
ISIRI*RN*GTLEEALLPAG*ALSKARGANE*T
PKNSESHTNPEEMDG*S*CFSEGGMACPWGFRN
SKKAAETVQTFSQ*YSDNSAQSKQCQ*RWAEDKE
*SRARVCFET*DRTQRT*HSVGSHVPTGLCQKG
GLEGRFGENCKPPERSIRDARMDDTS*RRVS*E
RF*I*NSR*ITESS*RDEES*RRGPTKRSESETP
Y*VCK*CHSSSSTCSTRGLKKGT*NSNHQLPVA
LH*AEWEMQDFGRSLGMLA*VIVILGESKQVAK
*SRI*T*NH*KHSWRS*GNL*GARFT*KFDATFR
G*PKSDSHIGTDPNRWRSHG*ANQ*GT*DI*FS
LEGTT*RGCKEAKVA*TEHPVCPGD*KILTLNP
GVPHIH*QAVGSLYCRQGGRSSNASGSPENPI*F
DKS*DQFRRNEET*SGEGGCPKSPVSD*CCTEK
ITRCLHEVSIIPETSQF*AASTRK*DDFR*SED
ALACIGNKECGTGSSTVTAKSLCELV*KSE*SEV
*SGNGDKDWTSDCTEKADGKSQRT**KSNSFEI
AL**AGSKGNRKKATVGEMLEIVP*DAKGNECL
DRMAGSYRYGIDKEISS*RNA**FGF*SCLGKGY
SKRD*ETEGAPEEYHRGRRGLENSFGQEGDVGG
R*TQSSE**LDSCHLPSRRVVKSFVGIPETHGN
F*PECGPHHKVDHSG*HTFG*IREKETPAKRRRA
*AFKGRTE*HTPKGGLYT*PSSKLDGKPR*PLQ
EISRAPNLRAQPSICSHFTQN*DWKGLHSFEGI
GAV*LRYTKIA*TTGG*NSAGGESERGRLQ*RYE
*RQ*GYCKRIVAKRRQLTTKNHR*EKARGNKDK
TAAVTDKT*CSQGFEVSKKKKGSRNFSSVVSVQ
EAG**SPEMLG*H*KKISQPT*AQR*KENKGN*S
GIAEEERGAECSA*AS*GLV*GWGRNGSGANSD
PAQQALAGN*EQICSVSKTQLCTNSHCP*RNDD
GDD*RHAFGNFLCAFYLFD*NHSCLTSPIRSGTT
SQCS*PLC*GL*RSL*ARGVSEEYKR*STTKLR
SD*HYS*QEDSSIAKCNACGKGEATGSSLPA*F
PMGKS*QNVQGPTRAI*QIC*EMAAFSL*YKDI*
SVANRS*TVSQKDTNS*ELGTC*IQMVS*GTPG
WHWAAANCCQNIECNWGRNNSAILKNRCQYSTG
KIGKPESAVAGGLQTAVRQKKEARRTKEYLVRIS
KRFK*ICFMVGGSR*HC*YPT*TWKRAATKRKA
*ASQVTGGRVAPAPGNSQTIK*NWRTRACKCSH
KPRRAR*T*K*AQADKSPVDKGFQSFT*ETRRN*
SSNKRPWAA*KKA*RP*RAVKSSAAVVISY*ES
VGNL*PTKPRRTI*R*GN*NSSSS*TTGCGRDF
V*RAAFVQGKTSHSASEEEVRRSEL*VEGGKPFT
SRAEGKAA*PSSWTDHYWSLSYSDCYSGDTTCG
Y*GNCHLQTRNAIFLDVGGTCSGRFQPGLDRTY
RLAFSA*SSYKITEGDGG*P*GYQRDDHQAEGNN
AGFGTEASPVGRTHYRCPKFEKQDQQSRG*NNH
YGSN*KNSESVG*STRTPSEPEATVE*NVKGFN
TMAGS*GRS*AGLRTGQSQA*VMEGGSLYSRCNP
KENHRNQAVGQRPPPVADKCRCGK*LGPETSPG
LFCR*YQKSPHDNREYQCLLEKHS*KGE*ARGC
FGRNS*ITATVPPGPGKVSCLAYRS*NNCQCPTG
CYP*GKAPRRLQGSKRADETMARPPR*N*SSHR
CLSQPG*KQPKNPEIPGRFR*CSPVTKTFG*HE
LQVE*TSEKVSQH*VPFGSQF*PVEASAPFSAGT
SGVATAER**IKPAGTYWRRLSSSSEAERCT*G
LQEGIEN*RTCNHEYS*DCTNISDRAAFGRTRE
TLPGAQRAAS*GESPECHSASTKAG*GGQY*VGK
IEPALR*LAEKNR*DP*KTPGTSRGHG*AGPQA
APS*GDQGILAARGRSPH*LSPRSPRESQGTSR
RNCASEREREPRQ*PCSPAYHFGHSALTV*PQHS
GRPEHQMEASAGGRRGPSQAAA*SPQGLWSSIS
ALSFHVCPGSLGESHLAKQSALLYQPRDSNNLL
GPSQNDRALPVFS*PE*CQILSL*DCHETPKTAE
GPLLGSLEPVSCM*CLGPAQPQAK*PAHGYPAD
Y*LFDHYL*PPGARAQQFGQRPSLRGYVSELAA
ECL*YGTNREDPCPVF*NWHHFPV*STFGRQVQI
PFQASGKFNRIL*PAQAGPPSA*FYPNSKTVG*
SCILWGQ*H*AKCPELLPIC***ARDRSGPLPR
LDETGTPVHGVAARPAQSGCCRNCQASGQM*HLQ
RVSNHWIQVQESKAL*L*HLPKLLFFWSSCKRP
*NALSHGGILHSDYIRRRCSRLCQGTKKQISNQ
KVFCEASPNGLPASADCLRGGQHGNSRYSDQLLA
SRFCACLVPSAFTR*YSFTH*TLC*QASRNGKQ
QWILSK**HLS**EHR**TFVNPALLPKFEPGL
PPEPAS*SCPDLDFLRE*GKRGARENPSRS*GRK
QESASRI*PSKAAART*RPVPTAVPS*NDAHLS
PESPGC*AHC*GQATASTQRPPGSQDANPGRPQ
*TAGVTVTQAKAAAGATPGRGQSEWHNGVLSFYL
STEVRQQSAYAAPSGWQSNFGLHG*GRSSQSSP
GHKHRVRGGDGATQQLLP*FKRKKYPWKANERG
HNVGSLFHMADDLGRAMESLVSVMTDEEGAE*MF
YNS*FPHGFYNIHTTKRIRQ*EFTRNKSIFL*R
VVVLYCRFQ*FLSLLLFC*QWQVLHVYAIVQKS
YKKTTCKILIAK*LAISLYGTHFGLFKNL*QL*R
KIVN*SVLYKKKLFIKTPKNKTNTHTHTYTHTH
KTLRQRIVLHPFGVISI*NSWLFLFLHIKDKTS
STTTPNDYYTLLI*ELSAEWGRLEFSFHISICL*
VYKYYSYIDKEIRISID*LFPFFKCSCHILIER
NYF*SVIQAYLLGLEWIFPGAGSQEETTPH*NI
VYSSRCFSF*TTFH*QRK*SKVLDFFKGNM*MNT
QDLLYQSE*SVGWLID*LIDTFSFLLLAMPRFR
FNDASVEINQKVF*PCEHQKVFFNSQAVAGR**
GWRAMDSQPIPVKE*ATL*VKDWMIVHNT*SSL*
LQLNYYALFSQSKGTGWFGFCCFFRFIVPCGMS
F*MPQDII*NK*TLGKGVKQ*PHHICDTDRYQP
RSP*TVFPSFAFLCE*FHTGL*VSKKEGKLIQML
QKNPSWWIRQVKYINKQTKIAQKRGEKLKRKS*
GLVGKSFTLSCHFISF*FLNHSFNRYHRVTYNF
ANLLPLTSSVISFWRVG*HQV*LAFGEWVLSIIN
N*LINIKHGFSCYFYLTLVLGCS**LCTPEFTA
SPLVHCVIFFFLYNSFFFLHNFQKKTQSSKVTN
YQIT*RFGFCLAFFSFM*RWTFSLPKDF*NSDLK
QGVTLHPTKKFK*VSFILKSEVNRVHK*FCFNL
FVFLLDTLALE*VCHNI*TKIESFIAAF*A*LI
WTLFRVVFFITTKY*TVNHNVTEA*TSHGMFCHC
FQVLSSYLSIIIYCVLTPTL*HLRIIFLNFSFT
AFSQHIRLHQIYALLLYYSTALLCISIKHAVML
Frame 2
AVCEAESVRTPHYSRQPEWKKQVQKDCVFVCFCEAGQRFCACFICAWL*LYLQVYHTP*NV*EKST
NREISKKLSYEGV*PASAEFEMSEVSSDEREDV
QKKTFTKWVNAQFSKFGKQHIENLFSDLQDGRRL
LDLLEGLTGQKLPKEKGSTRVHALNNVNKALRV
LQNNNVDLVNIGSTDIVDGNHKLTLGLIWNIIL
HWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRN
YPQVNVINFTTSWSDGLALNALIHSHRPDLFDW
NSVVCQQSATQRLEHAFNIARYQLGIEKLLDPE
DVDTTYPDKKSILMYITSLFQVLPQQVSIEAIQE
VEMLPRPPKVTKEEHFQLHHQMHYSQQITVSLA
QGYERTSSPKPRFKSYAYTQAAYVTTSDPTRSP
FPSQHLEAPEDKSFGSSLMESEVNLDRYQTALEE
VLSWLLSAEDTLQAQGEISNDVEVVKDQFHTHE
GYMMDLTAHQGRVGNILQLGSKLIGTGKLSEDE
ETEVQEQMNLLNSRWECLRVASMEKQSNLHRVLM
DLQNQKLKELNDWLTKTEERTRKMEEEPLGPDL
EDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVV
VDESSGDHATAALEEQLKVLGDRWANICRWTEDR
WVLLQDILLKWQRLTEEQCLFSAWLSEKEDAVN
KIHTTGFKDQNEMLSSLQKLAVLKADLEKKKQS
MGKLYSLKQDLLSTLKNKSVTQKTEAWLDNFARC
WDNLVQKLEKSTAQISQAVTTTQPSLTQTTVME
TVTTVTTREQILVKHAQEELPPPPPQKKRQITV
DSEIRKRLDVDITELHSWITRSEAVLQSPEFAIF
RKEGNFSDLKEKVNAIEREKAEKFRKLQDASRS
AQALVEQMVNEGVNADSIKQASEQLNSRWIEFC
QLLSERLNWLEYQNNIIAFYNQLQQLEQMTTTAE
NWLKIQPTTPSEPTAIKSQLKICKDEVNRLSDL
QPQIERLKIQSIALKEKGQGPMFLDADFVAFTN
HFKQVFSDVQAREKELQTIFDTLPPMRYQETMSA
IRTWVQQSETKLSIPQLSVTDYEIMEQRLGELQ
ALQSSLQEQQSGLYYLSTTVKEMSKKAPSEISR
KYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMNKL
RKIQNHIQTLKKWMAEVDVFLKEEWPALGDSEI
LKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIK
NEAEPEFASRLETELKELNTQWDHMCQQVYARKE
ALKGGLEKTVSLQKDLSEMHEWMTQAEEEYLER
DFEYKTPDELQKAVEEMKRAKEEAQQKEAKVKL
LTESVNSVIAQAPPVAQEALKKELETLTTNYQWL
CTRLNGKCKTLEEVWACWHELLSYLEKANKWLN
EVEFKLKTTENIPGGAEEISEVLDSLENLMRHS
EDNPNQIRILAQTLTDGGVMDELINEELETFNSR
WRELHEEAVRRQKLLEQSIQSAQETEKSLHLIQ
ESLTFIDKQLAAYIADKVDAAQMPQEAQKIQSD
LTSHEISLEEMKKHNQGKEAAQRVLSQIDVAQKK
LQDVSMKFRLFQKPANFEQRLQESKMILDEVKM
HLPALETKSVEQEVVQSQLNHCVNLYKSLSEVK
SEVEMVIKTGRQIVQKKQTENPKELDERVTALKL
HYNELGAKVTERKQQLEKCLKLSRKMRKEMNVL
TEWLAATDMELTKRSAVEGMPSNLDSEVAWGKA
TQKEIEKQKVHLKSITEVGEALKTVLGKKETLVE
DKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMET
FDQNVDHITKWIIQADTLLDESEKKKPQQKEDV
LKRLKAELNDIRPKVDSTRDQAANLMANRGDHCR
KLVEPQISELNHRFAAISHRIKTGKASIPLKEL
EQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDM
NEDNEGTVKELLQRGDNLQQRITDERKREEIKIK
QQLLQTKHNALKDLRSQRRKKALEISHQWYQYK
RQADDLLKCLDDIEKKLASLPEPRDERKIKEID
RELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQI
QLSKRWREIESKFAQFRRLNFAQIHTVREETMM
VMTEDMPLEISYVPSTYLTEITHVSQALLEVEQ
LLNAPDLCAKDFEDLFKQEESLKNIKDSLQQSSG
RIDIIHSKKTAALQSATPVERVKLQEALSQLDF
QWEKVNKMYKDRQGRFDRSVEKWRRFHYDIKIF
NQWLTEAEQFLRKTQIPENWEHAKYKWYLKELQD
GIGQRQTVVRTLNATGEEIIQQSSKTDASILQE
KLGSLNLRWQEVCKQLSDRKKRLEEQKNILSEF
QRDLNEFVLWLEEADNIASIPLEPGKEQQLKEKL
EQVKLLVEELPLRQGILKQLNETGGPVLVSAPI
SPEEQDKLENKLKQTNLQWIKVSRALPEKQGEI
EAQIKDLGQLEKKLEDLEEQLNHLLLWLSPIRNQ
LEIYNQPNQEGPFDVKETEIAVQAKQPDVEEIL
SKGQHLYKEKPATQPVKRKLEDLSSEWKAVNRL
LQELRAKQPDLAPGLTTIGASPTQTVTLVTQPVV
TKETAISKLEMPSSLMLEVPALADFNRAWTELT
DWLSLLDQVIKSQRVMVGDLEDINEMIIKQKAT
MQDLEQRRPQLEELITAAQNLKNKTSNQEARTII
TDRIERIQNQWDEVQEHLQNRRQQLNEMLKDST
QWLEAKEEAEQVLGQARAKLESWKEGPYTVDAI
QKKITETKQLAKDLRQWQTNVDVANDLALKLLRD
YSADDTRKVHMITENINASWRSIHKRVSEREAA
LEETHRLLQQFPLDLEKFLAWLTEAETTANVLQ
DATRKERLLEDSKGVKELMKQWQDLQGEIEAHTD
VYHNLDENSQKILRSLEGSDDAVLLQRRLDNMN
FKWSELRKKSLNIRSHLEASSDQWKRLHLSLQE
LLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRA
FKRELKTKEPVIMSTLETVRIFLTEQPLEGLEK
LYQEPRELPPEERAQNVTRLLRKQAEEVNTEWE
KLNLHSADWQRKIDETLERLRELQEATDELDLKL
RQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRG
EIAPLKENVSHVNDLARQLTTLGIQLSPYNLST
LEDLNTRWKLLQVAVEDRVRQLHEAHRDFGPASQ
HFLSTSVQGPWERAISPNKVPYYINHETQTTCW
DHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQ
KALCLDLLSLSAACDALDQHNLKQNDQPMDILQI
INCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLL
NVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYR
YLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGE
VASFGGSNIEPSVRSCFQFANNKPEIEAALFLD
WMRLEPQSMVWLPVLHRVAAAETAKHQAKCNIC
KECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGH
KMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTK
RYFAKHPRMGYLPVQTVLEGDNMETPVTLINFW
PVDSAPASSPQLSHDDTHSRIEHYASRLAEMENS
NGSYLNDSISPNESIDDEHLLIQHYCQSLNQDS
PLSQPRSPAQILISLESEERGELERILADLEEE
NRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSP
QSPRDAELIAEAKLLRQHKGRLEARMQILEDHN
KQLESQLHRLRQLLEQPQAEAKVNGTTVSSPST
SLQRSDSSQPMLLRVVGSQTSDSMGEEDLLSPPQ
DTSTGLEEVMEQLNNSFPSSRGRNTPGKPMRED
TM*EVFSTWQMIWAERWSP*YQS*QMKKEQNKC
FTTPDSRMVFIIFIQQRGLDSKSLQEINLYFCEG
*WYYTVDFSSF*VCYCFVNNGRFYTSMQLYKKV
IRKLHVKS**LNNLPFLYMERILGCLKIYNSYK
ERL*TKVCFIKKSCL*KPLKTKQTHTHTHTHTHT
KL*GSALFCILLA*YPYEIHGFFFFCILKIRLP
LPPHQMTTTHCSFENCQLSGAGLSFHFIYLYVY
KYINTIVI*IKRYEFL*TDFFHFLNVHVTS**KE
ITSSQSSRLTCLV*NGFFPEPEARRKLHHTKTL
STAPDVSHFKQLSTDNESKVKYWIFLKGTCE*I
HRTYYIRVSNRLVG*LID*LIHSASCC*QCHDLD
LMMLQWKSIRRYSDLVNIRRYFLTPKQ*QDDDR
AGGLWIPSPSL*RSRPLFK*RIG*LFIIHKVLC
NYN*IIMPSSHSQKELGGLVFVAFLDLLSHVG*V
FKCHKT*FKINKLWEKV*NSSPITFVILTGINP
EAHELCFHPLHFSASSSTQVCK*VRKKAN*FKC
YKKTLLGGLDRLNI*TNKQKLLKKEEKSSRGKAK
DW*EKALLFHAILFLFDF*IIHSIDTTV*PIIL
QICYL*HQV*LAFGEWADIKCN*LLESGFCPLL
IIN*LTSNTASHAISTSLWFWGVPDNCAHLSSQL
HHLSIALFSFSFIILSFSFIIFKRKPKALR*QI
TKLHEDLVFVLHFFPLCDAGPFLYPRIFKTQI*
NKGLLYILLRSLSK*VSF*NQR*IECINNFVLIF
LFFF*TH*LWSESVIIFEQKLRALLLHFKHN*F
GHYFVLCSL*PPSIKL*IIM*LKHKHHMACFVI
VFRY*VLT*VS*YIVF*HQHCNIYELFF*TSVLL
HFHNISDFTKYMPYYCIIVLLYCVSQ*STQLCY
Sequence NM_004007
ATGAAAGAGAAGATGTTCAAAAGAAAACATTCACAAAATGGGTAAATGCACAATTTTCTAAGTTTGGGAAGCAGCATATTGAGAACCTCTTCAGTGACCTACAGGATGGGAGGCGCCTCCTAGACCTCCTCGAAGGCCTGACAGGGCAAAAACTGCCAAAAGAAAAAGGATCCACAAGAGTTCATGCCCTGAACAATGTC
AACAAGGCACTGCGGGTTTTGCAGAACAATAATGTTGATTTAGTGAATATTGGAAGTACTGACATCGTAGATGGAAATCATAAACTGACTCTTGGTTTGA
TTTGGAATATAATCCTCCACTGGCAGGTCAAAAATGTAATGAAAAATATCATGGCTGGATTGCAACAAACCAACAGTGAAAAGATTCTCCTGAGCTGGGT
CCGACAATCAACTCGTAATTATCCACAGGTTAATGTAATCAACTTCACCACCAGCTGGTCTGATGGCCTGGCTTTGAATGCTCTCATCCATAGTCATAGG
CCAGACCTATTTGACTGGAATAGTGTGGTTTGCCAGCAGTCAGCCACACAACGACTGGAACATGCATTCAACATCGCCAGATATCAATTAGGCATAGAGA
AACTACTCGATCCTGAAGATGTTGATACCACCTATCCAGATAAGAAGTCCATCTTAATGTACATCACATCACTCTTCCAAGTTTTGCCTCAACAAGTGAG
CATTGAAGCCATCCAGGAAGTGGAAATGTTGCCAAGGCCACCTAAAGTGACTAAAGAAGAACATTTTCAGTTACATCATCAAATGCACTATTCTCAACAG
ATCACGGTCAGTCTAGCACAGGGATATGAGAGAACTTCTTCCCCTAAGCCTCGATTCAAGAGCTATGCCTACACACAGGCTGCTTATGTCACCACCTCTG
ACCCTACACGGAGCCCATTTCCTTCACAGCATTTGGAAGCTCCTGAAGACAAGTCATTTGGCAGTTCATTGATGGAGAGTGAAGTAAACCTGGACCGTTA
TCAAACAGCTTTAGAAGAAGTATTATCGTGGCTTCTTTCTGCTGAGGACACATTGCAAGCACAAGGAGAGATTTCTAATGATGTGGAAGTGGTGAAAGAC
CAGTTTCATACTCATGAGGGGTACATGATGGATTTGACAGCCCATCAGGGCCGGGTTGGTAATATTCTACAATTGGGAAGTAAGCTGATTGGAACAGGAA
AATTATCAGAAGATGAAGAAACTGAAGTACAAGAGCAGATGAATCTCCTAAATTCAAGATGGGAATGCCTCAGGGTAGCTAGCATGGAAAAACAAAGCAA
TTTACATAGAGTTTTAATGGATCTCCAGAATCAGAAACTGAAAGAGTTGAATGACTGGCTAACAAAAACAGAAGAAAGAACAAGGAAAATGGAGGAAGAG
CCTCTTGGACCTGATCTTGAAGACCTAAAACGCCAAGTACAACAACATAAGGTGCTTCAAGAAGATCTAGAACAAGAACAAGTCAGGGTCAATTCTCTCA
CTCACATGGTGGTGGTAGTTGATGAATCTAGTGGAGATCACGCAACTGCTGCTTTGGAAGAACAACTTAAGGTATTGGGAGATCGATGGGCAAACATCTG
TAGATGGACAGAAGACCGCTGGGTTCTTTTACAAGACATCCTTCTCAAATGGCAACGTCTTACTGAAGAACAGTGCCTTTTTAGTGCATGGCTTTCAGAA
AAAGAAGATGCAGTGAACAAGATTCACACAACTGGCTTTAAAGATCAAAATGAAATGTTATCAAGTCTTCAAAAACTGGCCGTTTTAAAAGCGGATCTAG
AAAAGAAAAAGCAATCCATGGGCAAACTGTATTCACTCAAACAAGATCTTCTTTCAACACTGAAGAATAAGTCAGTGACCCAGAAGACGGAAGCATGGCT
GGATAACTTTGCCCGGTGTTGGGATAATTTAGTCCAAAAACTTGAAAAGAGTACAGCACAGATTTCACAGGCTGTCACCACCACTCAGCCATCACTAACA
CAGACAACTGTAATGGAAACAGTAACTACGGTGACCACAAGGGAACAGATCCTGGTAAAGCATGCTCAAGAGGAACTTCCACCACCACCTCCCCAAAAGA
AGAGGCAGATTACTGTGGATTCTGAAATTAGGAAAAGGTTGGATGTTGATATAACTGAACTTCACAGCTGGATTACTCGCTCAGAAGCTGTGTTGCAGAG
TCCTGAATTTGCAATCTTTCGGAAGGAAGGCAACTTCTCAGACTTAAAAGAAAAAGTCAATGCCATAGAGCGAGAAAAAGCTGAGAAGTTCAGAAAACTG
CAAGATGCCAGCAGATCAGCTCAGGCCCTGGTGGAACAGATGGTGAATGAGGGTGTTAATGCAGATAGCATCAAACAAGCCTCAGAACAACTGAACAGCC
GGTGGATCGAATTCTGCCAGTTGCTAAGTGAGAGACTTAACTGGCTGGAGTATCAGAACAACATCATCGCTTTCTATAATCAGCTACAACAATTGGAGCA
GATGACAACTACTGCTGAAAACTGGTTGAAAATCCAACCCACCACCCCATCAGAGCCAACAGCAATTAAAAGTCAGTTAAAAATTTGTAAGGATGAAGTC
AACCGGCTATCAGATCTTCAACCTCAAATTGAACGATTAAAAATTCAAAGCATAGCCCTGAAAGAGAAAGGACAAGGACCCATGTTCCTGGATGCAGACT
TTGTGGCCTTTACAAATCATTTTAAGCAAGTCTTTTCTGATGTGCAGGCCAGAGAGAAAGAGCTACAGACAATTTTTGACACTTTGCCACCAATGCGCTA
TCAGGAGACCATGAGTGCCATCAGGACATGGGTCCAGCAGTCAGAAACCAAACTCTCCATACCTCAACTTAGTGTCACCGACTATGAAATCATGGAGCAG
AGACTCGGGGAATTGCAGGCTTTACAAAGTTCTCTGCAAGAGCAACAAAGTGGCCTATACTATCTCAGCACCACTGTGAAAGAGATGTCGAAGAAAGCGC
CCTCTGAAATTAGCCGGAAATATCAATCAGAATTTGAAGAAATTGAGGGACGCTGGAAGAAGCTCTCCTCCCAGCTGGTTGAGCATTGTCAAAAGCTAGA
GGAGCAAATGAATAAACTCCGAAAAATTCAGAATCACATACAAACCCTGAAGAAATGGATGGCTGAAGTTGATGTTTTTCTGAAGGAGGAATGGCCTGCC
CTTGGGGATTCAGAAATTCTAAAAAAGCAGCTGAAACAGTGCAGACTTTTAGTCAGTGATATTCAGACAATTCAGCCCAGTCTAAACAGTGTCAATGAAG
GTGGGCAGAAGATAAAGAATGAAGCAGAGCCAGAGTTTGCTTCGAGACTTGAGACAGAACTCAAAGAACTTAACACTCAGTGGGATCACATGTGCCAACA
GGTCTATGCCAGAAAGGAGGCCTTGAAGGGAGGTTTGGAGAAAACTGTAAGCCTCCAGAAAGATCTATCAGAGATGCACGAATGGATGACACAAGCTGAA
GAAGAGTATCTTGAGAGAGATTTTGAATATAAAACTCCAGATGAATTACAGAAAGCAGTTGAAGAGATGAAGAGAGCTAAAGAAGAGGCCCAACAAAAAG
AAGCGAAAGTGAAACTCCTTACTGAGTCTGTAAATAGTGTCATAGCTCAAGCTCCACCTGTAGCACAAGAGGCCTTAAAAAAGGAACTTGAAACTCTAAC
CACCAACTACCAGTGGCTCTGCACTAGGCTGAATGGGAAATGCAAGACTTTGGAAGAAGTTTGGGCATGTTGGCATGAGTTATTGTCATACTTGGAGAAA
GCAAACAAGTGGCTAAATGAAGTAGAATTTAAACTTAAAACCACTGAAAACATTCCTGGCGGAGCTGAGGAAATCTCTGAGGTGCTAGATTCACTTGAAA
ATTTGATGCGACATTCAGAGGATAACCCAAATCAGATTCGCATATTGGCACAGACCCTAACAGATGGCGGAGTCATGGATGAGCTAATCAATGAGGAACT
TGAGACATTTAATTCTCGTTGGAGGGAACTACATGAAGAGGCTGTAAGGAGGCAAAAGTTGCTTGAACAGAGCATCCAGTCTGCCCAGGAGACTGAAAAA
TCCTTACACTTAATCCAGGAGTCCCTCACATTCATTGACAAGCAGTTGGCAGCTTATATTGCAGACAAGGTGGACGCAGCTCAAATGCCTCAGGAAGCCC
AGAAAATCCAATCTGATTTGACAAGTCATGAGATCAGTTTAGAAGAAATGAAGAAACATAATCAGGGGAAGGAGGCTGCCCAAAGAGTCCTGTCTCAGAT
TGATGTTGCACAGAAAAAATTACAAGATGTCTCCATGAAGTTTCGATTATTCCAGAAACCAGCCAATTTTGAGCAGCGTCTACAAGAAAGTAAGATGATT
TTAGATGAAGTGAAGATGCACTTGCCTGCATTGGAAACAAAGAGTGTGGAACAGGAAGTAGTACAGTCACAGCTAAATCATTGTGTGAACTTGTATAAAA
GTCTGAGTGAAGTGAAGTCTGAAGTGGAAATGGTGATAAAGACTGGACGTCAGATTGTACAGAAAAAGCAGACGGAAAATCCCAAAGAACTTGATGAAAG
AGTAACAGCTTTGAAATTGCATTATAATGAGCTGGGAGCAAAGGTAACAGAAAGAAAGCAACAGTTGGAGAAATGCTTGAAATTGTCCCGTAAGATGCGA
AAGGAAATGAATGTCTTGACAGAATGGCTGGCAGCTACAGATATGGAATTGACAAAGAGATCAGCAGTTGAAGGAATGCCTAGTAATTTGGATTCTGAAG
TTGCCTGGGGAAAGGCTACTCAAAAAGAGATTGAGAAACAGAAGGTGCACCTGAAGAGTATCACAGAGGTAGGAGAGGCCTTGAAAACAGTTTTGGGCAA
GAAGGAGACGTTGGTGGAAGATAAACTCAGTCTTCTGAATAGTAACTGGATAGCTGTCACCTCCCGAGCAGAAGAGTGGTTAAATCTTTTGTTGGAATAC
CAGAAACACATGGAAACTTTTGACCAGAATGTGGACCACATCACAAAGTGGATCATTCAGGCTGACACACTTTTGGATGAATCAGAGAAAAAGAAACCCC
AGCAAAAAGAAGACGTGCTTAAGCGTTTAAAGGCAGAACTGAATGACATACGCCCAAAGGTGGACTCTACACGTGACCAAGCAGCAAACTTGATGGCAAA
CCGCGGTGACCACTGCAGGAAATTAGTAGAGCCCCAAATCTCAGAGCTCAACCATCGATTTGCAGCCATTTCACACAGAATTAAGACTGGAAAGGCCTCC
ATTCCTTTGAAGGAATTGGAGCAGTTTAACTCAGATATACAAAAATTGCTTGAACCACTGGAGGCTGAAATTCAGCAGGGGGTGAATCTGAAAGAGGAAG
ACTTCAATAAAGATATGAATGAAGACAATGAGGGTACTGTAAAAGAATTGTTGCAAAGAGGAGACAACTTACAACAAAGAATCACAGATGAGAGAAAGCG
AGAGGAAATAAAGATAAAACAGCAGCTGTTACAGACAAAACATAATGCTCTCAAGGATTTGAGGTCTCAAAGAAGAAAAAAGGCTCTAGAAATTTCTCAT
CAGTGGTATCAGTACAAGAGGCAGGCTGATGATCTCCTGAAATGCTTGGATGACATTGAAAAAAAATTAGCCAGCCTACCTGAGCCCAGAGATGAAAGGA
AAATAAAGGAAATTGATCGGGAATTGCAGAAGAAGAAAGAGGAGCTGAATGCAGTGCGTAGGCAAGCTGAGGGCTTGTCTGAGGATGGGGCCGCAATGGC
AGTGGAGCCAACTCAGATCCAGCTCAGCAAGCGCTGGCGGGAAATTGAGAGCAAATTTGCTCAGTTTCGAAGACTCAACTTTGCACAAATTCACACTGTC
CGTGAAGAAACGATGATGGTGATGACTGAAGACATGCCTTTGGAAATTTCTTATGTGCCTTCTACTTATTTGACTGAAATCACTCATGTCTCACAAGCCC
TATTAGAAGTGGAACAACTTCTCAATGCTCCTGACCTCTGTGCTAAGGACTTTGAAGATCTCTTTAAGCAAGAGGAGTCTCTGAAGAATATAAAAGATAG
TCTACAACAAAGCTCAGGTCGGATTGACATTATTCATAGCAAGAAGACAGCAGCATTGCAAAGTGCAACGCCTGTGGAAAGGGTGAAGCTACAGGAAGCT
CTCTCCCAGCTTGATTTCCAATGGGAAAAAGTTAACAAAATGTACAAGGACCGACAAGGGCGATTTGACAGATCTGTTGAGAAATGGCGGCGTTTTCATT
ATGATATAAAGATATTTAATCAGTGGCTAACAGAAGCTGAACAGTTTCTCAGAAAGACACAAATTCCTGAGAATTGGGAACATGCTAAATACAAATGGTA
TCTTAAGGAACTCCAGGATGGCATTGGGCAGCGGCAAACTGTTGTCAGAACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGAT
GCCAGTATTCTACAGGAAAAATTGGGAAGCCTGAATCTGCGGTGGCAGGAGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGGCTAGAAGAACAAAAGA
ATATCTTGTCAGAATTTCAAAGAGATTTAAATGAATTTGTTTTATGGTTGGAGGAAGCAGATAACATTGCTAGTATCCCACTTGAACCTGGAAAAGAGCA
GCAACTAAAAGAAAAGCTTGAGCAAGTCAAGTTACTGGTGGAAGAGTTGCCCCTGCGCCAGGGAATTCTCAAACAATTAAATGAAACTGGAGGACCCGTG
CTTGTAAGTGCTCCCATAAGCCCAGAAGAGCAAGATAAACTTGAAAATAAGCTCAAGCAGACAAATCTCCAGTGGATAAAGGTTTCCAGAGCTTTACCTG
AGAAACAAGGAGAAATTGAAGCTCAAATAAAAGACCTTGGGCAGCTTGAAAAAAAGCTTGAAGACCTTGAAGAGCAGTTAAATCATCTGCTGCTGTGGTT
ATCTCCTATTAGGAATCAGTTGGAAATTTATAACCAACCAAACCAAGAAGGACCATTTGACGTTAAGGAAACTGAAATAGCAGTTCAAGCTAAACAACCG
GATGTGGAAGAGATTTTGTCTAAAGGGCAGCATTTGTACAAGGAAAAACCAGCCACTCAGCCAGTGAAGAGGAAGTTAGAAGATCTGAGCTCTGAGTGGA
AGGCGGTAAACCGTTTACTTCAAGAGCTGAGGGCAAAGCAGCCTGACCTAGCTCCTGGACTGACCACTATTGGAGCCTCTCCTACTCAGACTGTTACTCT
GGTGACACAACCTGTGGTTACTAAGGAAACTGCCATCTCCAAACTAGAAATGCCATCTTCCTTGATGTTGGAGGTACCTGCTCTGGCAGATTTCAACCGG
GCTTGGACAGAACTTACCGACTGGCTTTCTCTGCTTGATCAAGTTATAAAATCACAGAGGGTGATGGTGGGTGACCTTGAGGATATCAACGAGATGATCA
TCAAGCAGAAGGCAACAATGCAGGATTTGGAACAGAGGCGTCCCCAGTTGGAAGAACTCATTACCGCTGCCCAAAATTTGAAAAACAAGACCAGCAATCA
AGAGGCTAGAACAATCATTACGGATCGAATTGAAAGAATTCAGAATCAGTGGGATGAAGTACAAGAACACCTTCAGAACCGGAGGCAACAGTTGAATGAA
ATGTTAAAGGATTCAACACAATGGCTGGAAGCTAAGGAAGAAGCTGAGCAGGTCTTAGGACAGGCCAGAGCCAAGCTTGAGTCATGGAAGGAGGGTCCCT
ATACAGTAGATGCAATCCAAAAGAAAATCACAGAAACCAAGCAGTTGGCCAAAGACCTCCGCCAGTGGCAGACAAATGTAGATGTGGCAAATGACTTGGC
CCTGAAACTTCTCCGGGATTATTCTGCAGATGATACCAGAAAAGTCCACATGATAACAGAGAATATCAATGCCTCTTGGAGAAGCATTCATAAAAGGGTG
AGTGAGCGAGAGGCTGCTTTGGAAGAAACTCATAGATTACTGCAACAGTTCCCCCTGGACCTGGAAAAGTTTCTTGCCTGGCTTACAGAAGCTGAAACAA
CTGCCAATGTCCTACAGGATGCTACCCGTAAGGAAAGGCTCCTAGAAGACTCCAAGGGAGTAAAAGAGCTGATGAAACAATGGCAAGACCTCCAAGGTGA
AATTGAAGCTCACACAGATGTTTATCACAACCTGGATGAAAACAGCCAAAAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTACAAAGA
CGTTTGGATAACATGAACTTCAAGTGGAGTGAACTTCGGAAAAAGTCTCTCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCGTCTGC
ACCTTTCTCTGCAGGAACTTCTGGTGTGGCTACAGCTGAAAGATGATGAATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAGAAGCA
GAACGATGTACATAGGGCCTTCAAGAGGGAATTGAAAACTAAAGAACCTGTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGCAGCCT
TTGGAAGGACTAGAGAAACTCTACCAGGAGCCCAGAGAGCTGCCTCCTGAGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGAGGAGG
TCAATACTGAGTGGGAAAAATTGAACCTGCACTCCGCTGACTGGCAGAGAAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCCACGGA
TGAGCTGGACCTCAAGCTGCGCCAAGCTGAGGTGATCAAGGGATCCTGGCAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCGAGAAA
GTCAAGGCACTTCGAGGAGAAATTGCGCCTCTGAAAGAGAACGTGAGCCACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCTCTCAC
CGTATAACCTCAGCACTCTGGAAGACCTGAACACCAGATGGAAGCTTCTGCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCACAGGGA
CTTTGGTCCAGCATCTCAGCACTTTCTTTCCACGTCTGTCCAGGGTCCCTGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACCACGAG
ACTCAAACAACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGCCATGA
AACTCCGAAGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGACCAGCC
CATGGATATCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGGATATG
TGTCTGAACTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGCACATT
TGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATCCAAAT
TCCAAGACAGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGATCGAA
GCGGCCCTCTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCATCAGG
CCAAATGTAACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGG
TCGAGTTGCAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTAAAA
AACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCCCGTTA
CTCTGATCAACTTCTGGCCAGTAGATTCTGCGCCTGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCT
AGCAGAAATGGAAAACAGCAATGGATCTTATCTAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAA
AGTTTGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAG
CAGATCTTGAGGAAGAAAACAGGAATCTGCAAGCAGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGA
AATGATGCCCACCTCTCCCCAGAGTCCCCGGGATGCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAA
ATCCTGGAAGACCACAATAAACAGCTGGAGTCACAGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGG
TGTCCTCTCCTTCTACCTCTCTACAGAGGTCCGACAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGA
TCTTCTCAGTCCTCCCCAGGACACAAGCACAGGGTTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCCCTGGA
AAGCCAATGAGAGAGGACACAATGTAGGAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGA
AGGAGCAGAATAAATGTTTTACAACTCCTGATTCCCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAA
TCTATATTTTTGTGAAGGGTAGTGGTATTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATG
CAATTGTACAAAAAAGTTATAAGAAAACTACATGTAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAA
TTTATAACAGTTATAAAGAAAGATTGTAAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACAC
ACATACACACACACACACAAAACTTTGAGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATA
TTAAAGATAAGACTTCCTCTACCACCACACCAAATGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCA
TATATCTATATGTCTATAAGTATATAAATACTATAGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGT
CACATCCTAATAGAAAGAAATTACTTCTAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTA
CACCACACTAAAACATTGTCTACAGCTCCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGG
GAACATGTGAATGAATACACAGGACTTATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTA
GCAATGCCACGATTTAGATTTAATGATGCTTCAGTGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAG
TAGCAGGACGATGATAGGGCTGGAGGGCTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAA
TACATAAAGTTCTCTGTAATTACAACTAAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATT
GTCCCATGTGGGATGAGTTTTTAAATGCCACAAGACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATA
CTGACAGGTATCAACCCAGAAGCCCATGAACTGTGTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGG
CAAATTGATTCAAATGTTACAAAAAAACCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAG
CTCAAGAGGAAAAGCTAAGGACTGGTAGGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACC
GTGTGACCTATAATTTTGCAAATCTGTTACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTG
GGTTTTGTCCATTATTAATAATTAATTAATTAACATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGC
ACACCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAA
GCTCTAAGGTAACAAATTACCAAATTACATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTT
TTAAAACTCAGATTTAAAACAAGGGGTTACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAA
TAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAGACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCAT
TTTAAGCATAATTAATTTGGACATTATTTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACA
TGGCATGTTTTGTCATTGTTTTCAGGTACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTT
TTAAACTTCAGTTTTACTGCATTTTCACAACATATCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAA
AGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
MKEKMFKRKHSQNG*MHNFLSLGSSILRTSSVTYRMGGAS*TSSKA*QGKNCQKKKDPQEFMP*TMS
TRHCGFCRTIMLI**ILEVLTS*MEIIN*LLV*
FGI*SSTGRSKM**KISWLDCNKPTVKRFS*AG
SDNQLVIIHRLM*STSPPAGLMAWL*MLSSIVIG
QTYLTGIVWFASSQPHNDWNMHSTSPDIN*A*R
NYSILKMLIPPIQIRSPS*CTSHHSSKFCLNK*
ALKPSRKWKCCQGHLK*LKKNIFSYIIKCTILNR
SRSV*HRDMRELLPLSLDSRAMPTHRLLMSPPL
TLHGAHFLHSIWKLLKTSHLAVH*WRVK*TWTV
IKQL*KKYYRGFFLLRTHCKHKERFLMMWKW*KT
SFILMRGT*WI*QPIRAGLVIFYNWEVS*LEQE
NYQKMKKLKYKSR*IS*IQDGNASG*LAWKNKA
IYIEF*WISRIRN*KS*MTG*QKQKKEQGKWRKS
LLDLILKT*NAKYNNIRCFKKI*NKNKSGSILS
LTWWW*LMNLVEITQLLLWKNNLRYWEIDGQTS
VDGQKTAGFFYKTSFSNGNVLLKNSAFLVHGFQK
KKMQ*TRFTQLALKIKMKCYQVFKNWPF*KRI*
KRKSNPWANCIHSNKIFFQH*RISQ*PRRRKHG
WITLPGVGII*SKNLKRVQHRFHRLSPPLSHH*H
RQL*WKQ*LR*PQGNRSW*SMLKRNFHHHLPKR
RGRLLWILKLGKGWMLI*LNFTAGLLAQKLCCR
VLNLQSFGRKATSQT*KKKSMP*SEKKLRSSENC
KMPADQLRPWWNRW*MRVLMQIASNKPQNN*TA
GGSNSASC*VRDLTGWSIRTTSSLSIISYNNWS
R*QLLLKTG*KSNPPPHQSQQQLKVS*KFVRMKS
TGYQIFNLKLND*KFKA*P*KRKDKDPCSWMQT
LWPLQIILSKSFLMCRPERKSYRQFLTLCHQCA
IRRP*VPSGHGSSSQKPNSPYLNLVSPTMKSWSR
DSGNCRLYKVLCKSNKVAYTISAPL*KRCRRKR
PLKLAGNINQNLKKLRDAGRSSPPSWLSIVKS*
RSK*INSEKFRITYKP*RNGWLKLMFF*RRNGLP
LGIQKF*KSS*NSADF*SVIFRQFSPV*TVSMK
VGRR*RMKQSQSLLRDLRQNSKNLTLSGITCAN
RSMPERRP*REVWRKL*ASRKIYQRCTNG*HKLK
KSILREILNIKLQMNYRKQLKR*RELKKRPNKK
KRK*NSLLSL*IVS*LKLHL*HKRP*KRNLKL*
PPTTSGSALG*MGNARLWKKFGHVGMSYCHTWRK
QTSG*MK*NLNLKPLKTFLAELRKSLRC*IHLK
I*CDIQRITQIRFAYWHRP*QMAESWMS*SMRN
LRHLILVGGNYMKRL*GGKSCLNRASSLPRRLKN
PYT*SRSPSHSLTSSWQLILQTRWTQLKCLRKP
RKSNLI*QVMRSV*KK*RNIIRGRRLPKESCLR
LMLHRKNYKMSP*SFDYSRNQPILSSVYKKVR*F
*MK*RCTCLHWKQRVWNRK*YSHS*IIV*TCIK
V*VK*SLKWKW**RLDVRLYRKSRRKIPKNLMK
E*QL*NCIIMSWEQR*QKESNSWRNA*NCPVRCE
RK*MS*QNGWQLQIWN*QRDQQLKECLVIWILK
LPGERLLKKRLRNRRCT*RVSQR*ERP*KQFWA
RRRRWWKINSVF*IVTG*LSPPEQKSG*IFCWNT
RNTWKLLTRMWTTSQSGSFRLTHFWMNQRKRNP
SKKKTCLSV*RQN*MTYAQRWTLHVTKQQT*WQ
TAVTTAGN**SPKSQSSTIDLQPFHTELRLERPP
FL*RNWSSLTQIYKNCLNHWRLKFSRG*I*KRK
TSIKI*MKTMRVL*KNCCKEETTYNKESQMRES
ERK*R*NSSCYRQNIMLSRI*GLKEEKRL*KFLI
SGISTRGRLMIS*NAWMTLKKN*PAYLSPEMKG
K*RKLIGNCRRRKRS*MQCVGKLRACLRMGPQW
QWSQLRSSSASAGGKLRANLLSFEDSTLHKFTLS
VKKR*W**LKTCLWKFLMCLLLI*LKSLMSHKP
Y*KWNNFSMLLTSVLRTLKISLSKRSL*RI*KI
VYNKAQVGLTLFIARRQQHCKVQRLWKG*SYRKL
SPSLISNGKKLTKCTRTDKGDLTDLLRNGGVFI
MI*RYLISG*QKLNSFSERHKFLRIGNMLNTNG
ILRNSRMALGSGKLLSEH*MQLGKK*FSNPQKQM
PVFYRKNWEA*ICGGRRSANSCQTEKRG*KNKR
ISCQNFKEI*MNLFYGWRKQITLLVSHLNLEKS
SN*KKSLSKSSYWWKSCPCAREFSNN*MKLEDPC
L*VLP*AQKSKINLKISSSRQISSG*RFPELYL
RNKEKLKLK*KTLGSLKKSLKTLKSS*IICCCG
YLLLGISWKFITNQTKKDHLTLRKLK*QFKLNNR
MWKRFCLKGSICTRKNQPLSQ*RGS*KI*ALSG
RR*TVYFKS*GQSSLT*LLD*PLLEPLLLRLLL
W*HNLWLLRKLPSPN*KCHLP*CWRYLLWQISTG
LGQNLPTGFLCLIKL*NHRG*WWVTLRISTR*S
SSRRQQCRIWNRGVPSWKNSLPLPKI*KTRPAI
KRLEQSLRIELKEFRISGMKYKNTFRTGGNS*MK
C*RIQHNGWKLRKKLSRS*DRPEPSLSHGRRVP
IQ*MQSKRKSQKPSSWPKTSASGRQM*MWQMTW
P*NFSGIILQMIPEKST**QRISMPLGEAFIKG*
VSERLLWKKLIDYCNSSPWTWKSFLPGLQKLKQ
LPMSYRMLPVRKGS*KTPRE*KS**NNGKTSKV
KLKLTQMFITTWMKTAKKS*DPWKVPMMQSCYKD
VWIT*TSSGVNFGKSLSTLGPIWKPVLTSGSVC
TFLCRNFWCGYS*KMMN*AGRHLLEATFQQFRS
RTMYIGPSRGN*KLKNL*S*VLLRLYEYF*QSSL
WKD*RNSTRSPESCLLRREPRMSLGFYESRLRR
SILSGKN*TCTPLTGREK*MRPLKDSGNFKRPR
MSWTSSCAKLR*SRDPGSPWAISSLTLSKITSRK
SRHFEEKLRL*KRT*ATSMTLLASLPLWAFSSH
RITSALWKT*TPDGSFCRWPSRTESGSCMKPTG
TLVQHLSTFFPRLSRVPGREPSRQTKCPTISTTR
LKQLAGTIPK*QSSTSL*LT*IMSDSQLIGLP*
NSEDCRRPFAWIS*ACQLHVMPWTSTTSSKMTS
PWISCRLLIV*PLFMTAWSKSTTIWSTSLSAWIC
V*TGC*MFMIRDEQGGSVSCLLKLASFPCVKHI
WKTSTDTFSSKWQVQQDFVTSAGWASFCMILSK
FQDSWVKLHPLGAVTLSQVSGAASNLLIISQRSK
RPSS*TG*DWNPSPWCGCPSCTEWLLQKLPSIR
PNVTSAKSVQSLDSGTGV*STLIMTSAKAAFFL
VELQKAIKCTIPWWNIALRLHQEKMFETLPRY*K
TNFEPKGILRSIPEWATCQCRLS*RGTTWKLPL
L*STSGQ*ILRLPRPLSFHTMILIHALNIMLAG
*QKWKTAMDLI*MIASLLMRA*MMNIC*SSITAK
V*TRTPP*ASLVVLPRS*FP*RVRKEGS*RES*
QILRKKTGICKQNMTV*SSSTNIKACPHCRPLL
K*CPPLPRVPGMLSSLLRPSYCVNTKAAWKPGCK
SWKTTINSWSHSYTG*GSCWSNPRQRPK*MAQR
CPLLLPLYRGPTAVSLCCSEWLAVKLRTPWVRK
IFSVLPRTQAQG*RR*WSNSTTPSLVQEEEIPLE
SQ*ERTQCRKSFPHGR*FGQSDGVLSISHDR*R
RSRINVLQLLIPAWFL*YSYNKED*TVRVYKK*
IYIFVKGSGIIL*ISVVSKSVIVLLTMAGFTRLC
NCTKKL*ENYM*NLDS*ITCHFFIWNAFWVV*K
FITVIKKDCKLKCAL*KKVVYKNP*KQNKHTHT
HIHTHTQNFEAAHCFASFWRDIHMKFMAFSFFAY
*R*DFLYHHTK*LLHTAHLRTVS*VGQA*VFIS
YIYMSISI*IL*LYR*RDTNFYRLTFSIF*MFM
SHPNRKKLLLVSHPGLPAWSRMDFSRSRKPGGNY
TTLKHCLQLQMFLILNNFPLTTKVK*SIGFF*R
EHVNEYTGLIISE*VIGWLVD*LID*YIQLPAA
SNATI*I**CFSGNQSEGILTL*TSEGIF*LPSS
SRTMIGLEGYGFPAHPCEGVGHSLSEGLDDCS*
YIKFSVITTKLLCPLLTVKRNWVVWFLLLF*IY
CPMWDEFLNATRHNLK*INFGKRCKTVAPSHL*Y
*QVSTQKPMNCVSILCISLRVVPHRFVSK*ERR
QIDSNVTKKPFLVD*TG*IYKQTNKNCSKKRRK
AQEEKLRTGRKKLYSFMPFYFFLIFKSFIQ*IPP
CDL*FCKSVTSDIKCN*LLESGLTSSVISFWRV
GFVHY**LIN*HQTRLLMLFLPHFGFGVFLIIV
HT*VHSFTTCPLRYFLFPL*FFLFPS*FSKENPK
L*GNKLPNYMKIWFLSCIFFLYVTLDLFFTQGF
LKLRFKTRGYFTSY*EV*VSKFHSKIRGK*SA*
IILF*SFCFSFRHISSGVSLS*YLNKN*ELYCCI
LSIINLDIISCCVLYNHQVLNCKS*CN*SINIT
WHVLSLFSGTEFLLEYHNILCFNTNTVTFTNYF
FKLQFYCIFTTYQTSPNICLTIVL*YCFTVYLNK
ARSYV
Frame 1
*KRRCSKENIHKMGKCTIF*VWEAAY*EPLQ*PTGWEAPPRPPRRPDRAKTAKRKRIHKSSCPEQC
QQGTAGFAEQ*C*FSEYWKY*HRRWKS*TDSWFD
LEYNPPLAGQKCNEKYHGWIATNQQ*KDSPELG
PTINS*LSTG*CNQLHHQLV*WPGFECSHP*S*
ARPI*LE*CGLPAVSHTTTGTCIQHRQISIRHRE
TTRS*RC*YHLSR*EVHLNVHHITLPSFASTSE
H*SHPGSGNVAKAT*SD*RRTFSVTSSNALFST
DHGQSSTGI*ENFFP*ASIQELCLHTGCLCHHL*
PYTEPISFTAFGSS*RQVIWQFIDGE*SKPGPL
SNSFRRSIIVASFC*GHIASTRRDF**CGSGER
PVSYS*GVHDGFDSPSGPGW*YSTIGK*ADWNRK
IIRR*RN*STRADESPKFKMGMPQGS*HGKTKQ
FT*SFNGSPESETERVE*LANKNRRKNKENGGR
ASWT*S*RPKTPSTTT*GASRRSRTRTSQGQFSH
SHGGGS**I*WRSRNCCFGRTT*GIGRSMGKHL
*MDRRPLGSFTRHPSQMATSY*RTVPF*CMAFR
KRRCSEQDSHNWL*RSK*NVIKSSKTGRFKSGSR
KEKAIHGQTVFTQTRSSFNTEE*VSDPEDGSMA
G*LCPVLG*FSPKT*KEYSTDFTGCHHHSAITN
TDNCNGNSNYGDHKGTDPGKACSRGTSTTTSPKE
EADYCGF*N*EKVGC*YN*TSQLDYSLRSCVAE
S*ICNLSEGRQLLRLKRKSQCHRARKS*EVQKT
ARCQQISSGPGGTDGE*GC*CR*HQTSLRTTEQP
VDRILPVAK*ET*LAGVSEQHHRFL*SATTIGA
DDNYC*KLVENPTHHPIRANSN*KSVKNL*G*S
QPAIRSSTSN*TIKNSKHSPERERTRTHVPGCRL
CGLYKSF*ASLF*CAGQRERATDNF*HFATNAL
SGDHECHQDMGPAVRNQTLHTST*CHRL*NHGA
ETRGIAGFTKFSARATKWPILSQHHCERDVEESA
L*N*PEISIRI*RN*GTLEEALLPAG*ALSKAR
GANE*TPKNSESHTNPEEMDG*S*CFSEGGMAC
PWGFRNSKKAAETVQTFSQ*YSDNSAQSKQCQ*R
WAEDKE*SRARVCFET*DRTQRT*HSVGSHVPT
GLCQKGGLEGRFGENCKPPERSIRDARMDDTS*
RRVS*ERF*I*NSR*ITESS*RDEES*RRGPTKR
SESETPY*VCK*CHSSSSTCSTRGLKKGT*NSN
HQLPVALH*AEWEMQDFGRSLGMLA*VIVILGE
SKQVAK*SRI*T*NH*KHSWRS*GNL*GARFT*K
FDATFRG*PKSDSHIGTDPNRWRSHG*ANQ*GT
*DI*FSLEGTT*RGCKEAKVA*TEHPVCPGD*K
ILTLNPGVPHIH*QAVGSLYCRQGGRSSNASGSP
ENPI*FDKS*DQFRRNEET*SGEGGCPKSPVSD
*CCTEKITRCLHEVSIIPETSQF*AASTRK*DD
FR*SEDALACIGNKECGTGSSTVTAKSLCELV*K
SE*SEV*SGNGDKDWTSDCTEKADGKSQRT**K
SNSFEIAL**AGSKGNRKKATVGEMLEIVP*DA
KGNECLDRMAGSYRYGIDKEISS*RNA**FGF*S
CLGKGYSKRD*ETEGAPEEYHRGRRGLENSFGQ
EGDVGGR*TQSSE**LDSCHLPSRRVVKSFVGI
PETHGNF*PECGPHHKVDHSG*HTFG*IREKETP
AKRRRA*AFKGRTE*HTPKGGLYT*PSSKLDGK
PR*PLQEISRAPNLRAQPSICSHFTQN*DWKGL
HSFEGIGAV*LRYTKIA*TTGG*NSAGGESERGR
LQ*RYE*RQ*GYCKRIVAKRRQLTTKNHR*EKA
RGNKDKTAAVTDKT*CSQGFEVSKKKKGSRNFS
SVVSVQEAG**SPEMLG*H*KKISQPT*AQR*KE
NKGN*SGIAEEERGAECSA*AS*GLV*GWGRNG
SGANSDPAQQALAGN*EQICSVSKTQLCTNSHC
P*RNDDGDD*RHAFGNFLCAFYLFD*NHSCLTSP
IRSGTTSQCS*PLC*GL*RSL*ARGVSEEYKR*
STTKLRSD*HYS*QEDSSIAKCNACGKGEATGS
SLPA*FPMGKS*QNVQGPTRAI*QIC*EMAAFSL
*YKDI*SVANRS*TVSQKDTNS*ELGTC*IQMV
S*GTPGWHWAAANCCQNIECNWGRNNSAILKNR
CQYSTGKIGKPESAVAGGLQTAVRQKKEARRTKE
YLVRISKRFK*ICFMVGGSR*HC*YPT*TWKRA
ATKRKA*ASQVTGGRVAPAPGNSQTIK*NWRTR
ACKCSHKPRRAR*T*K*AQADKSPVDKGFQSFT*
ETRRN*SSNKRPWAA*KKA*RP*RAVKSSAAVV
ISY*ESVGNL*PTKPRRTI*R*GN*NSSSS*TT
GCGRDFV*RAAFVQGKTSHSASEEEVRRSEL*VE
GGKPFTSRAEGKAA*PSSWTDHYWSLSYSDCYS
GDTTCGY*GNCHLQTRNAIFLDVGGTCSGRFQP
GLDRTYRLAFSA*SSYKITEGDGG*P*GYQRDDH
QAEGNNAGFGTEASPVGRTHYRCPKFEKQDQQS
RG*NNHYGSN*KNSESVG*STRTPSEPEATVE*
NVKGFNTMAGS*GRS*AGLRTGQSQA*VMEGGSL
YSRCNPKENHRNQAVGQRPPPVADKCRCGK*LG
PETSPGLFCR*YQKSPHDNREYQCLLEKHS*KG
E*ARGCFGRNS*ITATVPPGPGKVSCLAYRS*NN
CQCPTGCYP*GKAPRRLQGSKRADETMARPPR*
N*SSHRCLSQPG*KQPKNPEIPGRFR*CSPVTK
TFG*HELQVE*TSEKVSQH*VPFGSQF*PVEASA
PFSAGTSGVATAER**IKPAGTYWRRLSSSSEA
ERCT*GLQEGIEN*RTCNHEYS*DCTNISDRAA
FGRTRETLPGAQRAAS*GESPECHSASTKAG*GG
QY*VGKIEPALR*LAEKNR*DP*KTPGTSRGHG
*AGPQAAPS*GDQGILAARGRSPH*LSPRSPRE
SQGTSRRNCASEREREPRQ*PCSPAYHFGHSALT
V*PQHSGRPEHQMEASAGGRRGPSQAAA*SPQG
LWSSISALSFHVCPGSLGESHLAKQSALLYQPR
DSNNLLGPSQNDRALPVFS*PE*CQILSL*DCHE
TPKTAEGPLLGSLEPVSCM*CLGPAQPQAK*PA
HGYPADY*LFDHYL*PPGARAQQFGQRPSLRGY
VSELAAECL*YGTNREDPCPVF*NWHHFPV*STF
GRQVQIPFQASGKFNRIL*PAQAGPPSA*FYPN
SKTVG*SCILWGQ*H*AKCPELLPIC***ARDR
SGPLPRLDETGTPVHGVAARPAQSGCCRNCQASG
QM*HLQRVSNHWIQVQESKAL*L*HLPKLLFFW
SSCKRP*NALSHGGILHSDYIRRRCSRLCQGTK
KQISNQKVFCEASPNGLPASADCLRGGQHGNSRY
SDQLLASRFCACLVPSAFTR*YSFTH*TLC*QA
SRNGKQQWILSK**HLS**EHR**TFVNPALLP
KFEPGLPPEPAS*SCPDLDFLRE*GKRGARENPS
RS*GRKQESASRI*PSKAAART*RPVPTAVPS*
NDAHLSPESPGC*AHC*GQATASTQRPPGSQDA
NPGRPQ*TAGVTVTQAKAAAGATPGRGQSEWHNG
VLSFYLSTEVRQQSAYAAPSGWQSNFGLHG*GR
SSQSSPGHKHRVRGGDGATQQLLP*FKRKKYPW
KANERGHNVGSLFHMADDLGRAMESLVSVMTDEE
GAE*MFYNS*FPHGFYNIHTTKRIRQ*EFTRNK
SIFL*RVVVLYCRFQ*FLSLLLFC*QWQVLHVY
AIVQKSYKKTTCKILIAK*LAISLYGTHFGLFKN
L*QL*RKIVN*SVLYKKKLFIKTPKNKTNTHTH
TYTHTHKTLRQRIVLHPFGVISI*NSWLFLFLH
IKDKTSSTTTPNDYYTLLI*ELSAEWGRLEFSFH
ISICL*VYKYYSYIDKEIRISID*LFPFFKCSC
HILIERNYF*SVIQAYLLGLEWIFPGAGSQEET
TPH*NIVYSSRCFSF*TTFH*QRK*SKVLDFFKG
NM*MNTQDLLYQSE*SVGWLID*LIDTFSFLLL
AMPRFRFNDASVEINQKVF*PCEHQKVFFNSQA
VAGR**GWRAMDSQPIPVKE*ATL*VKDWMIVHN
T*SSL*LQLNYYALFSQSKGTGWFGFCCFFRFI
VPCGMSF*MPQDII*NK*TLGKGVKQ*PHHICD
TDRYQPRSP*TVFPSFAFLCE*FHTGL*VSKKEG
KLIQMLQKNPSWWIRQVKYINKQTKIAQKRGEK
LKRKS*GLVGKSFTLSCHFISF*FLNHSFNRYH
RVTYNFANLLPLTSSVISFWRVG*HQV*LAFGEW
VLSIINN*LINIKHGFSCYFYLTLVLGCS**LC
TPEFTASPLVHCVIFFFLYNSFFFLHNFQKKTQ
SSKVTNYQIT*RFGFCLAFFSFM*RWTFSLPKDF
*NSDLKQGVTLHPTKKFK*VSFILKSEVNRVHK
*FCFNLFVFLLDTLALE*VCHNI*TKIESFIAA
F*A*LIWTLFRVVFFITTKY*TVNHNVTEA*TSH
GMFCHCFQVLSSYLSIIIYCVLTPTL*HLRIIF
LNFSFTAFSQHIRLHQIYALLLYYSTALLCISI
KHAVML
Frame 2
EREDVQKKTFTKWVNAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNV
NKALRVLQNNNVDLVNIGSTDIVDGNHKLTLGL
IWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWV
RQSTRNYPQVNVINFTTSWSDGLALNALIHSHR
PDLFDWNSVVCQQSATQRLEHAFNIARYQLGIE
KLLDPEDVDTTYPDKKSILMYITSLFQVLPQQVS
IEAIQEVEMLPRPPKVTKEEHFQLHHQMHYSQQ
ITVSLAQGYERTSSPKPRFKSYAYTQAAYVTTS
DPTRSPFPSQHLEAPEDKSFGSSLMESEVNLDRY
QTALEEVLSWLLSAEDTLQAQGEISNDVEVVKD
QFHTHEGYMMDLTAHQGRVGNILQLGSKLIGTG
KLSEDEETEVQEQMNLLNSRWECLRVASMEKQSN
LHRVLMDLQNQKLKELNDWLTKTEERTRKMEEE
PLGPDLEDLKRQVQQHKVLQEDLEQEQVRVNSL
THMVVVVDESSGDHATAALEEQLKVLGDRWANIC
RWTEDRWVLLQDILLKWQRLTEEQCLFSAWLSE
KEDAVNKIHTTGFKDQNEMLSSLQKLAVLKADL
EKKKQSMGKLYSLKQDLLSTLKNKSVTQKTEAWL
DNFARCWDNLVQKLEKSTAQISQAVTTTQPSLT
QTTVMETVTTVTTREQILVKHAQEELPPPPPQK
KRQITVDSEIRKRLDVDITELHSWITRSEAVLQS
PEFAIFRKEGNFSDLKEKVNAIEREKAEKFRKL
QDASRSAQALVEQMVNEGVNADSIKQASEQLNS
RWIEFCQLLSERLNWLEYQNNIIAFYNQLQQLEQ
MTTTAENWLKIQPTTPSEPTAIKSQLKICKDEV
NRLSDLQPQIERLKIQSIALKEKGQGPMFLDAD
FVAFTNHFKQVFSDVQAREKELQTIFDTLPPMRY
QETMSAIRTWVQQSETKLSIPQLSVTDYEIMEQ
RLGELQALQSSLQEQQSGLYYLSTTVKEMSKKA
PSEISRKYQSEFEEIEGRWKKLSSQLVEHCQKLE
EQMNKLRKIQNHIQTLKKWMAEVDVFLKEEWPA
LGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNE
GGQKIKNEAEPEFASRLETELKELNTQWDHMCQQ
VYARKEALKGGLEKTVSLQKDLSEMHEWMTQAE
EEYLERDFEYKTPDELQKAVEEMKRAKEEAQQK
EAKVKLLTESVNSVIAQAPPVAQEALKKELETLT
TNYQWLCTRLNGKCKTLEEVWACWHELLSYLEK
ANKWLNEVEFKLKTTENIPGGAEEISEVLDSLE
NLMRHSEDNPNQIRILAQTLTDGGVMDELINEEL
ETFNSRWRELHEEAVRRQKLLEQSIQSAQETEK
SLHLIQESLTFIDKQLAAYIADKVDAAQMPQEA
QKIQSDLTSHEISLEEMKKHNQGKEAAQRVLSQI
DVAQKKLQDVSMKFRLFQKPANFEQRLQESKMI
LDEVKMHLPALETKSVEQEVVQSQLNHCVNLYK
SLSEVKSEVEMVIKTGRQIVQKKQTENPKELDER
VTALKLHYNELGAKVTERKQQLEKCLKLSRKMR
KEMNVLTEWLAATDMELTKRSAVEGMPSNLDSE
VAWGKATQKEIEKQKVHLKSITEVGEALKTVLGK
KETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEY
QKHMETFDQNVDHITKWIIQADTLLDESEKKKP
QQKEDVLKRLKAELNDIRPKVDSTRDQAANLMAN
RGDHCRKLVEPQISELNHRFAAISHRIKTGKAS
IPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEE
DFNKDMNEDNEGTVKELLQRGDNLQQRITDERKR
EEIKIKQQLLQTKHNALKDLRSQRRKKALEISH
QWYQYKRQADDLLKCLDDIEKKLASLPEPRDER
KIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMA
VEPTQIQLSKRWREIESKFAQFRRLNFAQIHTV
REETMMVMTEDMPLEISYVPSTYLTEITHVSQA
LLEVEQLLNAPDLCAKDFEDLFKQEESLKNIKDS
LQQSSGRIDIIHSKKTAALQSATPVERVKLQEA
LSQLDFQWEKVNKMYKDRQGRFDRSVEKWRRFH
YDIKIFNQWLTEAEQFLRKTQIPENWEHAKYKWY
LKELQDGIGQRQTVVRTLNATGEEIIQQSSKTD
ASILQEKLGSLNLRWQEVCKQLSDRKKRLEEQK
NILSEFQRDLNEFVLWLEEADNIASIPLEPGKEQ
QLKEKLEQVKLLVEELPLRQGILKQLNETGGPV
LVSAPISPEEQDKLENKLKQTNLQWIKVSRALP
EKQGEIEAQIKDLGQLEKKLEDLEEQLNHLLLWL
SPIRNQLEIYNQPNQEGPFDVKETEIAVQAKQP
DVEEILSKGQHLYKEKPATQPVKRKLEDLSSEW
KAVNRLLQELRAKQPDLAPGLTTIGASPTQTVTL
VTQPVVTKETAISKLEMPSSLMLEVPALADFNR
AWTELTDWLSLLDQVIKSQRVMVGDLEDINEMI
IKQKATMQDLEQRRPQLEELITAAQNLKNKTSNQ
EARTIITDRIERIQNQWDEVQEHLQNRRQQLNE
MLKDSTQWLEAKEEAEQVLGQARAKLESWKEGP
YTVDAIQKKITETKQLAKDLRQWQTNVDVANDLA
LKLLRDYSADDTRKVHMITENINASWRSIHKRV
SEREAALEETHRLLQQFPLDLEKFLAWLTEAET
TANVLQDATRKERLLEDSKGVKELMKQWQDLQGE
IEAHTDVYHNLDENSQKILRSLEGSDDAVLLQR
RLDNMNFKWSELRKKSLNIRSHLEASSDQWKRL
HLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQ
NDVHRAFKRELKTKEPVIMSTLETVRIFLTEQP
LEGLEKLYQEPRELPPEERAQNVTRLLRKQAEE
VNTEWEKLNLHSADWQRKIDETLERLRELQEATD
ELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEK
VKALRGEIAPLKENVSHVNDLARQLTTLGIQLS
PYNLSTLEDLNTRWKLLQVAVEDRVRQLHEAHRD
FGPASQHFLSTSVQGPWERAISPNKVPYYINHE
TQTTCWDHPKMTELYQSLADLNNVRFSAYRTAM
KLRRLQKALCLDLLSLSAACDALDQHNLKQNDQP
MDILQIINCLTTIYDRLEQEHNNLVNVPLCVDM
CLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAH
LEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQI
PRQLGEVASFGGSNIEPSVRSCFQFANNKPEIE
AALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQ
AKCNICKECPIIGFRYRSLKHFNYDICQSCFFSG
RVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLK
NKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPV
TLINFWPVDSAPASSPQLSHDDTHSRIEHYASRL
AEMENSNGSYLNDSISPNESIDDEHLLIQHYCQ
SLNQDSPLSQPRSPAQILISLESEERGELERIL
ADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPE
MMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQ
ILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTT
VSSPSTSLQRSDSSQPMLLRVVGSQTSDSMGEED
LLSPPQDTSTGLEEVMEQLNNSFPSSRGRNTPG
KPMREDTM*EVFSTWQMIWAERWSP*YQS*QMK
KEQNKCFTTPDSRMVFIIFIQQRGLDSKSLQEIN
LYFCEG*WYYTVDFSSF*VCYCFVNNGRFYTSM
QLYKKVIRKLHVKS**LNNLPFLYMERILGCLK
IYNSYKERL*TKVCFIKKSCL*KPLKTKQTHTHT
HTHTHTKL*GSALFCILLA*YPYEIHGFFFFCI
LKIRLPLPPHQMTTTHCSFENCQLSGAGLSFHF
IYLYVYKYINTIVI*IKRYEFL*TDFFHFLNVHV
TS**KEITSSQSSRLTCLV*NGFFPEPEARRKL
HHTKTLSTAPDVSHFKQLSTDNESKVKYWIFLK
GTCE*IHRTYYIRVSNRLVG*LID*LIHSASCC*
QCHDLDLMMLQWKSIRRYSDLVNIRRYFLTPKQ
*QDDDRAGGLWIPSPSL*RSRPLFK*RIG*LFI
IHKVLCNYN*IIMPSSHSQKELGGLVFVAFLDLL
SHVG*VFKCHKT*FKINKLWEKV*NSSPITFVI
LTGINPEAHELCFHPLHFSASSSTQVCK*VRKK
AN*FKCYKKTLLGGLDRLNI*TNKQKLLKKEEKS
SRGKAKDW*EKALLFHAILFLFDF*IIHSIDTT
V*PIILQICYL*HQV*LAFGEWADIKCN*LLES
GFCPLLIIN*LTSNTASHAISTSLWFWGVPDNCA
HLSSQLHHLSIALFSFSFIILSFSFIIFKRKPK
ALR*QITKLHEDLVFVLHFFPLCDAGPFLYPRI
FKTQI*NKGLLYILLRSLSK*VSF*NQR*IECIN
NFVLIFLFFF*TH*LWSESVIIFEQKLRALLLH
FKHN*FGHYFVLCSL*PPSIKL*IIM*LKHKHH
MACFVIVFRY*VLT*VS*YIVF*HQHCNIYELFF
*TSVLLHFHNISDFTKYMPYYCIIVLLYCVSQ*
STQLCY
Sequence NM_004006
TCCTGGCATCAGTTACTGTGTTGACTCACTCAGTGTTGGGATCACTCACTTTCCCCCTACAGGACTCAGATCTGGGAGGCAATTACCTTCGGAGAAAAACGAATAGGAAAAACTGAAGTGTTACTTTTTTTAAAGCTGCTGAAGTTTGTTGGTTTCTCATTGTTTTTAAGCCTACTGGAGCAATAAAGTTTGAAGAACTT
TTACCAGGTTTTTTTTATCGCTGCCTTGATATACACTTTTCAAAATGCTTTGGTGGGAAGAAGTAGAGGACTGTTATGAAAGAGAAGATGTTCAAAAGAA
AACATTCACAAAATGGGTAAATGCACAATTTTCTAAGTTTGGGAAGCAGCATATTGAGAACCTCTTCAGTGACCTACAGGATGGGAGGCGCCTCCTAGAC
CTCCTCGAAGGCCTGACAGGGCAAAAACTGCCAAAAGAAAAAGGATCCACAAGAGTTCATGCCCTGAACAATGTCAACAAGGCACTGCGGGTTTTGCAGA
ACAATAATGTTGATTTAGTGAATATTGGAAGTACTGACATCGTAGATGGAAATCATAAACTGACTCTTGGTTTGATTTGGAATATAATCCTCCACTGGCA
GGTCAAAAATGTAATGAAAAATATCATGGCTGGATTGCAACAAACCAACAGTGAAAAGATTCTCCTGAGCTGGGTCCGACAATCAACTCGTAATTATCCA
CAGGTTAATGTAATCAACTTCACCACCAGCTGGTCTGATGGCCTGGCTTTGAATGCTCTCATCCATAGTCATAGGCCAGACCTATTTGACTGGAATAGTG
TGGTTTGCCAGCAGTCAGCCACACAACGACTGGAACATGCATTCAACATCGCCAGATATCAATTAGGCATAGAGAAACTACTCGATCCTGAAGATGTTGA
TACCACCTATCCAGATAAGAAGTCCATCTTAATGTACATCACATCACTCTTCCAAGTTTTGCCTCAACAAGTGAGCATTGAAGCCATCCAGGAAGTGGAA
ATGTTGCCAAGGCCACCTAAAGTGACTAAAGAAGAACATTTTCAGTTACATCATCAAATGCACTATTCTCAACAGATCACGGTCAGTCTAGCACAGGGAT
ATGAGAGAACTTCTTCCCCTAAGCCTCGATTCAAGAGCTATGCCTACACACAGGCTGCTTATGTCACCACCTCTGACCCTACACGGAGCCCATTTCCTTC
ACAGCATTTGGAAGCTCCTGAAGACAAGTCATTTGGCAGTTCATTGATGGAGAGTGAAGTAAACCTGGACCGTTATCAAACAGCTTTAGAAGAAGTATTA
TCGTGGCTTCTTTCTGCTGAGGACACATTGCAAGCACAAGGAGAGATTTCTAATGATGTGGAAGTGGTGAAAGACCAGTTTCATACTCATGAGGGGTACA
TGATGGATTTGACAGCCCATCAGGGCCGGGTTGGTAATATTCTACAATTGGGAAGTAAGCTGATTGGAACAGGAAAATTATCAGAAGATGAAGAAACTGA
AGTACAAGAGCAGATGAATCTCCTAAATTCAAGATGGGAATGCCTCAGGGTAGCTAGCATGGAAAAACAAAGCAATTTACATAGAGTTTTAATGGATCTC
CAGAATCAGAAACTGAAAGAGTTGAATGACTGGCTAACAAAAACAGAAGAAAGAACAAGGAAAATGGAGGAAGAGCCTCTTGGACCTGATCTTGAAGACC
TAAAACGCCAAGTACAACAACATAAGGTGCTTCAAGAAGATCTAGAACAAGAACAAGTCAGGGTCAATTCTCTCACTCACATGGTGGTGGTAGTTGATGA
ATCTAGTGGAGATCACGCAACTGCTGCTTTGGAAGAACAACTTAAGGTATTGGGAGATCGATGGGCAAACATCTGTAGATGGACAGAAGACCGCTGGGTT
CTTTTACAAGACATCCTTCTCAAATGGCAACGTCTTACTGAAGAACAGTGCCTTTTTAGTGCATGGCTTTCAGAAAAAGAAGATGCAGTGAACAAGATTC
ACACAACTGGCTTTAAAGATCAAAATGAAATGTTATCAAGTCTTCAAAAACTGGCCGTTTTAAAAGCGGATCTAGAAAAGAAAAAGCAATCCATGGGCAA
ACTGTATTCACTCAAACAAGATCTTCTTTCAACACTGAAGAATAAGTCAGTGACCCAGAAGACGGAAGCATGGCTGGATAACTTTGCCCGGTGTTGGGAT
AATTTAGTCCAAAAACTTGAAAAGAGTACAGCACAGATTTCACAGGCTGTCACCACCACTCAGCCATCACTAACACAGACAACTGTAATGGAAACAGTAA
CTACGGTGACCACAAGGGAACAGATCCTGGTAAAGCATGCTCAAGAGGAACTTCCACCACCACCTCCCCAAAAGAAGAGGCAGATTACTGTGGATTCTGA
AATTAGGAAAAGGTTGGATGTTGATATAACTGAACTTCACAGCTGGATTACTCGCTCAGAAGCTGTGTTGCAGAGTCCTGAATTTGCAATCTTTCGGAAG
GAAGGCAACTTCTCAGACTTAAAAGAAAAAGTCAATGCCATAGAGCGAGAAAAAGCTGAGAAGTTCAGAAAACTGCAAGATGCCAGCAGATCAGCTCAGG
CCCTGGTGGAACAGATGGTGAATGAGGGTGTTAATGCAGATAGCATCAAACAAGCCTCAGAACAACTGAACAGCCGGTGGATCGAATTCTGCCAGTTGCT
AAGTGAGAGACTTAACTGGCTGGAGTATCAGAACAACATCATCGCTTTCTATAATCAGCTACAACAATTGGAGCAGATGACAACTACTGCTGAAAACTGG
TTGAAAATCCAACCCACCACCCCATCAGAGCCAACAGCAATTAAAAGTCAGTTAAAAATTTGTAAGGATGAAGTCAACCGGCTATCAGATCTTCAACCTC
AAATTGAACGATTAAAAATTCAAAGCATAGCCCTGAAAGAGAAAGGACAAGGACCCATGTTCCTGGATGCAGACTTTGTGGCCTTTACAAATCATTTTAA
GCAAGTCTTTTCTGATGTGCAGGCCAGAGAGAAAGAGCTACAGACAATTTTTGACACTTTGCCACCAATGCGCTATCAGGAGACCATGAGTGCCATCAGG
ACATGGGTCCAGCAGTCAGAAACCAAACTCTCCATACCTCAACTTAGTGTCACCGACTATGAAATCATGGAGCAGAGACTCGGGGAATTGCAGGCTTTAC
AAAGTTCTCTGCAAGAGCAACAAAGTGGCCTATACTATCTCAGCACCACTGTGAAAGAGATGTCGAAGAAAGCGCCCTCTGAAATTAGCCGGAAATATCA
ATCAGAATTTGAAGAAATTGAGGGACGCTGGAAGAAGCTCTCCTCCCAGCTGGTTGAGCATTGTCAAAAGCTAGAGGAGCAAATGAATAAACTCCGAAAA
ATTCAGAATCACATACAAACCCTGAAGAAATGGATGGCTGAAGTTGATGTTTTTCTGAAGGAGGAATGGCCTGCCCTTGGGGATTCAGAAATTCTAAAAA
AGCAGCTGAAACAGTGCAGACTTTTAGTCAGTGATATTCAGACAATTCAGCCCAGTCTAAACAGTGTCAATGAAGGTGGGCAGAAGATAAAGAATGAAGC
AGAGCCAGAGTTTGCTTCGAGACTTGAGACAGAACTCAAAGAACTTAACACTCAGTGGGATCACATGTGCCAACAGGTCTATGCCAGAAAGGAGGCCTTG
AAGGGAGGTTTGGAGAAAACTGTAAGCCTCCAGAAAGATCTATCAGAGATGCACGAATGGATGACACAAGCTGAAGAAGAGTATCTTGAGAGAGATTTTG
AATATAAAACTCCAGATGAATTACAGAAAGCAGTTGAAGAGATGAAGAGAGCTAAAGAAGAGGCCCAACAAAAAGAAGCGAAAGTGAAACTCCTTACTGA
GTCTGTAAATAGTGTCATAGCTCAAGCTCCACCTGTAGCACAAGAGGCCTTAAAAAAGGAACTTGAAACTCTAACCACCAACTACCAGTGGCTCTGCACT
AGGCTGAATGGGAAATGCAAGACTTTGGAAGAAGTTTGGGCATGTTGGCATGAGTTATTGTCATACTTGGAGAAAGCAAACAAGTGGCTAAATGAAGTAG
AATTTAAACTTAAAACCACTGAAAACATTCCTGGCGGAGCTGAGGAAATCTCTGAGGTGCTAGATTCACTTGAAAATTTGATGCGACATTCAGAGGATAA
CCCAAATCAGATTCGCATATTGGCACAGACCCTAACAGATGGCGGAGTCATGGATGAGCTAATCAATGAGGAACTTGAGACATTTAATTCTCGTTGGAGG
GAACTACATGAAGAGGCTGTAAGGAGGCAAAAGTTGCTTGAACAGAGCATCCAGTCTGCCCAGGAGACTGAAAAATCCTTACACTTAATCCAGGAGTCCC
TCACATTCATTGACAAGCAGTTGGCAGCTTATATTGCAGACAAGGTGGACGCAGCTCAAATGCCTCAGGAAGCCCAGAAAATCCAATCTGATTTGACAAG
TCATGAGATCAGTTTAGAAGAAATGAAGAAACATAATCAGGGGAAGGAGGCTGCCCAAAGAGTCCTGTCTCAGATTGATGTTGCACAGAAAAAATTACAA
GATGTCTCCATGAAGTTTCGATTATTCCAGAAACCAGCCAATTTTGAGCAGCGTCTACAAGAAAGTAAGATGATTTTAGATGAAGTGAAGATGCACTTGC
CTGCATTGGAAACAAAGAGTGTGGAACAGGAAGTAGTACAGTCACAGCTAAATCATTGTGTGAACTTGTATAAAAGTCTGAGTGAAGTGAAGTCTGAAGT
GGAAATGGTGATAAAGACTGGACGTCAGATTGTACAGAAAAAGCAGACGGAAAATCCCAAAGAACTTGATGAAAGAGTAACAGCTTTGAAATTGCATTAT
AATGAGCTGGGAGCAAAGGTAACAGAAAGAAAGCAACAGTTGGAGAAATGCTTGAAATTGTCCCGTAAGATGCGAAAGGAAATGAATGTCTTGACAGAAT
GGCTGGCAGCTACAGATATGGAATTGACAAAGAGATCAGCAGTTGAAGGAATGCCTAGTAATTTGGATTCTGAAGTTGCCTGGGGAAAGGCTACTCAAAA
AGAGATTGAGAAACAGAAGGTGCACCTGAAGAGTATCACAGAGGTAGGAGAGGCCTTGAAAACAGTTTTGGGCAAGAAGGAGACGTTGGTGGAAGATAAA
CTCAGTCTTCTGAATAGTAACTGGATAGCTGTCACCTCCCGAGCAGAAGAGTGGTTAAATCTTTTGTTGGAATACCAGAAACACATGGAAACTTTTGACC
AGAATGTGGACCACATCACAAAGTGGATCATTCAGGCTGACACACTTTTGGATGAATCAGAGAAAAAGAAACCCCAGCAAAAAGAAGACGTGCTTAAGCG
TTTAAAGGCAGAACTGAATGACATACGCCCAAAGGTGGACTCTACACGTGACCAAGCAGCAAACTTGATGGCAAACCGCGGTGACCACTGCAGGAAATTA
GTAGAGCCCCAAATCTCAGAGCTCAACCATCGATTTGCAGCCATTTCACACAGAATTAAGACTGGAAAGGCCTCCATTCCTTTGAAGGAATTGGAGCAGT
TTAACTCAGATATACAAAAATTGCTTGAACCACTGGAGGCTGAAATTCAGCAGGGGGTGAATCTGAAAGAGGAAGACTTCAATAAAGATATGAATGAAGA
CAATGAGGGTACTGTAAAAGAATTGTTGCAAAGAGGAGACAACTTACAACAAAGAATCACAGATGAGAGAAAGCGAGAGGAAATAAAGATAAAACAGCAG
CTGTTACAGACAAAACATAATGCTCTCAAGGATTTGAGGTCTCAAAGAAGAAAAAAGGCTCTAGAAATTTCTCATCAGTGGTATCAGTACAAGAGGCAGG
CTGATGATCTCCTGAAATGCTTGGATGACATTGAAAAAAAATTAGCCAGCCTACCTGAGCCCAGAGATGAAAGGAAAATAAAGGAAATTGATCGGGAATT
GCAGAAGAAGAAAGAGGAGCTGAATGCAGTGCGTAGGCAAGCTGAGGGCTTGTCTGAGGATGGGGCCGCAATGGCAGTGGAGCCAACTCAGATCCAGCTC
AGCAAGCGCTGGCGGGAAATTGAGAGCAAATTTGCTCAGTTTCGAAGACTCAACTTTGCACAAATTCACACTGTCCGTGAAGAAACGATGATGGTGATGA
CTGAAGACATGCCTTTGGAAATTTCTTATGTGCCTTCTACTTATTTGACTGAAATCACTCATGTCTCACAAGCCCTATTAGAAGTGGAACAACTTCTCAA
TGCTCCTGACCTCTGTGCTAAGGACTTTGAAGATCTCTTTAAGCAAGAGGAGTCTCTGAAGAATATAAAAGATAGTCTACAACAAAGCTCAGGTCGGATT
GACATTATTCATAGCAAGAAGACAGCAGCATTGCAAAGTGCAACGCCTGTGGAAAGGGTGAAGCTACAGGAAGCTCTCTCCCAGCTTGATTTCCAATGGG
AAAAAGTTAACAAAATGTACAAGGACCGACAAGGGCGATTTGACAGATCTGTTGAGAAATGGCGGCGTTTTCATTATGATATAAAGATATTTAATCAGTG
GCTAACAGAAGCTGAACAGTTTCTCAGAAAGACACAAATTCCTGAGAATTGGGAACATGCTAAATACAAATGGTATCTTAAGGAACTCCAGGATGGCATT
GGGCAGCGGCAAACTGTTGTCAGAACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGATGCCAGTATTCTACAGGAAAAATTGG
GAAGCCTGAATCTGCGGTGGCAGGAGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGGCTAGAAGAACAAAAGAATATCTTGTCAGAATTTCAAAGAGA
TTTAAATGAATTTGTTTTATGGTTGGAGGAAGCAGATAACATTGCTAGTATCCCACTTGAACCTGGAAAAGAGCAGCAACTAAAAGAAAAGCTTGAGCAA
GTCAAGTTACTGGTGGAAGAGTTGCCCCTGCGCCAGGGAATTCTCAAACAATTAAATGAAACTGGAGGACCCGTGCTTGTAAGTGCTCCCATAAGCCCAG
AAGAGCAAGATAAACTTGAAAATAAGCTCAAGCAGACAAATCTCCAGTGGATAAAGGTTTCCAGAGCTTTACCTGAGAAACAAGGAGAAATTGAAGCTCA
AATAAAAGACCTTGGGCAGCTTGAAAAAAAGCTTGAAGACCTTGAAGAGCAGTTAAATCATCTGCTGCTGTGGTTATCTCCTATTAGGAATCAGTTGGAA
ATTTATAACCAACCAAACCAAGAAGGACCATTTGACGTTAAGGAAACTGAAATAGCAGTTCAAGCTAAACAACCGGATGTGGAAGAGATTTTGTCTAAAG
GGCAGCATTTGTACAAGGAAAAACCAGCCACTCAGCCAGTGAAGAGGAAGTTAGAAGATCTGAGCTCTGAGTGGAAGGCGGTAAACCGTTTACTTCAAGA
GCTGAGGGCAAAGCAGCCTGACCTAGCTCCTGGACTGACCACTATTGGAGCCTCTCCTACTCAGACTGTTACTCTGGTGACACAACCTGTGGTTACTAAG
GAAACTGCCATCTCCAAACTAGAAATGCCATCTTCCTTGATGTTGGAGGTACCTGCTCTGGCAGATTTCAACCGGGCTTGGACAGAACTTACCGACTGGC
TTTCTCTGCTTGATCAAGTTATAAAATCACAGAGGGTGATGGTGGGTGACCTTGAGGATATCAACGAGATGATCATCAAGCAGAAGGCAACAATGCAGGA
TTTGGAACAGAGGCGTCCCCAGTTGGAAGAACTCATTACCGCTGCCCAAAATTTGAAAAACAAGACCAGCAATCAAGAGGCTAGAACAATCATTACGGAT
CGAATTGAAAGAATTCAGAATCAGTGGGATGAAGTACAAGAACACCTTCAGAACCGGAGGCAACAGTTGAATGAAATGTTAAAGGATTCAACACAATGGC
TGGAAGCTAAGGAAGAAGCTGAGCAGGTCTTAGGACAGGCCAGAGCCAAGCTTGAGTCATGGAAGGAGGGTCCCTATACAGTAGATGCAATCCAAAAGAA
AATCACAGAAACCAAGCAGTTGGCCAAAGACCTCCGCCAGTGGCAGACAAATGTAGATGTGGCAAATGACTTGGCCCTGAAACTTCTCCGGGATTATTCT
GCAGATGATACCAGAAAAGTCCACATGATAACAGAGAATATCAATGCCTCTTGGAGAAGCATTCATAAAAGGGTGAGTGAGCGAGAGGCTGCTTTGGAAG
AAACTCATAGATTACTGCAACAGTTCCCCCTGGACCTGGAAAAGTTTCTTGCCTGGCTTACAGAAGCTGAAACAACTGCCAATGTCCTACAGGATGCTAC
CCGTAAGGAAAGGCTCCTAGAAGACTCCAAGGGAGTAAAAGAGCTGATGAAACAATGGCAAGACCTCCAAGGTGAAATTGAAGCTCACACAGATGTTTAT
CACAACCTGGATGAAAACAGCCAAAAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTACAAAGACGTTTGGATAACATGAACTTCAAGT
GGAGTGAACTTCGGAAAAAGTCTCTCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCGTCTGCACCTTTCTCTGCAGGAACTTCTGGT
GTGGCTACAGCTGAAAGATGATGAATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAGAAGCAGAACGATGTACATAGGGCCTTCAAG
AGGGAATTGAAAACTAAAGAACCTGTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGCAGCCTTTGGAAGGACTAGAGAAACTCTACC
AGGAGCCCAGAGAGCTGCCTCCTGAGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGAGGAGGTCAATACTGAGTGGGAAAAATTGAA
CCTGCACTCCGCTGACTGGCAGAGAAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCCACGGATGAGCTGGACCTCAAGCTGCGCCAA
GCTGAGGTGATCAAGGGATCCTGGCAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCGAGAAAGTCAAGGCACTTCGAGGAGAAATTG
CGCCTCTGAAAGAGAACGTGAGCCACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCTCTCACCGTATAACCTCAGCACTCTGGAAGA
CCTGAACACCAGATGGAAGCTTCTGCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCACAGGGACTTTGGTCCAGCATCTCAGCACTTT
CTTTCCACGTCTGTCCAGGGTCCCTGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACCACGAGACTCAAACAACTTGCTGGGACCATC
CCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGCCATGAAACTCCGAAGACTGCAGAAGGCCCT
TTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGACCAGCCCATGGATATCCTGCAGATTATTAAT
TGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGGATATGTGTCTGAACTGGCTGCTGAATGTTT
ATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGCACATTTGGAAGACAAGTACAGATACCTTTT
CAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATCCAAATTCCAAGACAGTTGGGTGAAGTTGCA
TCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGATCGAAGCGGCCCTCTTCCTAGACTGGATGA
GACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCATCAGGCCAAATGTAACATCTGCAAAGAGTG
TCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGTCGAGTTGCAAAAGGCCATAAAATG
CACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTAAAAAACAAATTTCGAACCAAAAGGTATT
TTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCCCGTTACTCTGATCAACTTCTGGCCAGTAGA
TTCTGCGCCTGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCAGAAATGGAAAACAGCAATGGA
TCTTATCTAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTTTGAACCAGGACTCCCCCCTGA
GCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAAACAGGAA
TCTGCAAGCAGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATGATGCCCACCTCTCCCCAGAGT
CCCCGGGATGCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCCTGGAAGACCACAATAAACAGC
TGGAGTCACAGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTCCTCTCCTTCTACCTCTCTACA
GAGGTCCGACAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTTCTCAGTCCTCCCCAGGACACA
AGCACAGGGTTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCCCTGGAAAGCCAATGAGAGAGGACACAATGT
AGGAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGAGCAGAATAAATGTTTTACAAC
TCCTGATTCCCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTATATTTTTGTGAAGGGTAGTGG
TATTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTTATAAGAA
AACTACATGTAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAGAAAGATT
GTAAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACATACACACACACACACAAAACTT
TGAGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAAAGATAAGACTTCCTCTACCAC
CACACCAAATGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATAAGTATAT
AAATACTATAGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGAAATTACT
TCTAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACCACACTAAAACATTGTCTACAG
CTCCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAACATGTGAATGAATACACAGGAC
TTATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGATTTAATG
ATGCTTCAGTGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGGGCTGGAG
GGCTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACATAAAGTTCTCTGTAATTACAA
CTAAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCCCATGTGGGATGAGTTTTTAAA
TGCCACAAGACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGGTATCAACCCAGAAGCCC
ATGAACTGTGTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTTACAAAAA
AACCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCAAGAGGAAAAGCTAAGGACTGG
TAGGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACCGTGTGACCTATAATTTTGCAAATCT
GTTACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAATAATTAA
TTAATTAACATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCTTCACCAC
TTGTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTCTAAGGTAACAAATTACCAAAT
TACATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAAAACTCAGATTTAAAACAAGGG
GTTACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTTTTTGTTT
TTCTTTTAGACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTTGGACATT
ATTTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGCATGTTTTGTCATTGTTTTCAG
GTACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTTTTAAACTTCAGTTTTACTGCATTTT
CACAACATATCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
SWHQLLC*LTQCWDHSLSPYRTQIWEAITFGEKRIGKTEVLLFLKLLKFVGFSLFLSLLEQ*SLKNF
YQVFFIAALIYTFQNALVGRSRGLL*KRRCSKE
NIHKMGKCTIF*VWEAAY*EPLQ*PTGWEAPPR
PPRRPDRAKTAKRKRIHKSSCPEQCQQGTAGFAE
Q*C*FSEYWKY*HRRWKS*TDSWFDLEYNPPLA
GQKCNEKYHGWIATNQQ*KDSPELGPTINS*LS
TG*CNQLHHQLV*WPGFECSHP*S*ARPI*LE*C
GLPAVSHTTTGTCIQHRQISIRHRETTRS*RC*
YHLSR*EVHLNVHHITLPSFASTSEH*SHPGSG
NVAKAT*SD*RRTFSVTSSNALFSTDHGQSSTGI
*ENFFP*ASIQELCLHTGCLCHHL*PYTEPISF
TAFGSS*RQVIWQFIDGE*SKPGPLSNSFRRSI
IVASFC*GHIASTRRDF**CGSGERPVSYS*GVH
DGFDSPSGPGW*YSTIGK*ADWNRKIIRR*RN*
STRADESPKFKMGMPQGS*HGKTKQFT*SFNGS
PESETERVE*LANKNRRKNKENGGRASWT*S*RP
KTPSTTT*GASRRSRTRTSQGQFSHSHGGGS**
I*WRSRNCCFGRTT*GIGRSMGKHL*MDRRPLG
SFTRHPSQMATSY*RTVPF*CMAFRKRRCSEQDS
HNWL*RSK*NVIKSSKTGRFKSGSRKEKAIHGQ
TVFTQTRSSFNTEE*VSDPEDGSMAG*LCPVLG
*FSPKT*KEYSTDFTGCHHHSAITNTDNCNGNSN
YGDHKGTDPGKACSRGTSTTTSPKEEADYCGF*
N*EKVGC*YN*TSQLDYSLRSCVAES*ICNLSE
GRQLLRLKRKSQCHRARKS*EVQKTARCQQISSG
PGGTDGE*GC*CR*HQTSLRTTEQPVDRILPVA
K*ET*LAGVSEQHHRFL*SATTIGADDNYC*KL
VENPTHHPIRANSN*KSVKNL*G*SQPAIRSSTS
N*TIKNSKHSPERERTRTHVPGCRLCGLYKSF*
ASLF*CAGQRERATDNF*HFATNALSGDHECHQ
DMGPAVRNQTLHTST*CHRL*NHGAETRGIAGFT
KFSARATKWPILSQHHCERDVEESAL*N*PEIS
IRI*RN*GTLEEALLPAG*ALSKARGANE*TPK
NSESHTNPEEMDG*S*CFSEGGMACPWGFRNSKK
AAETVQTFSQ*YSDNSAQSKQCQ*RWAEDKE*S
RARVCFET*DRTQRT*HSVGSHVPTGLCQKGGL
EGRFGENCKPPERSIRDARMDDTS*RRVS*ERF*
I*NSR*ITESS*RDEES*RRGPTKRSESETPY*
VCK*CHSSSSTCSTRGLKKGT*NSNHQLPVALH
*AEWEMQDFGRSLGMLA*VIVILGESKQVAK*SR
I*T*NH*KHSWRS*GNL*GARFT*KFDATFRG*
PKSDSHIGTDPNRWRSHG*ANQ*GT*DI*FSLE
GTT*RGCKEAKVA*TEHPVCPGD*KILTLNPGVP
HIH*QAVGSLYCRQGGRSSNASGSPENPI*FDK
S*DQFRRNEET*SGEGGCPKSPVSD*CCTEKIT
RCLHEVSIIPETSQF*AASTRK*DDFR*SEDALA
CIGNKECGTGSSTVTAKSLCELV*KSE*SEV*S
GNGDKDWTSDCTEKADGKSQRT**KSNSFEIAL
**AGSKGNRKKATVGEMLEIVP*DAKGNECLDRM
AGSYRYGIDKEISS*RNA**FGF*SCLGKGYSK
RD*ETEGAPEEYHRGRRGLENSFGQEGDVGGR*
TQSSE**LDSCHLPSRRVVKSFVGIPETHGNF*P
ECGPHHKVDHSG*HTFG*IREKETPAKRRRA*A
FKGRTE*HTPKGGLYT*PSSKLDGKPR*PLQEI
SRAPNLRAQPSICSHFTQN*DWKGLHSFEGIGAV
*LRYTKIA*TTGG*NSAGGESERGRLQ*RYE*R
Q*GYCKRIVAKRRQLTTKNHR*EKARGNKDKTA
AVTDKT*CSQGFEVSKKKKGSRNFSSVVSVQEAG
**SPEMLG*H*KKISQPT*AQR*KENKGN*SGI
AEEERGAECSA*AS*GLV*GWGRNGSGANSDPA
QQALAGN*EQICSVSKTQLCTNSHCP*RNDDGDD
*RHAFGNFLCAFYLFD*NHSCLTSPIRSGTTSQ
CS*PLC*GL*RSL*ARGVSEEYKR*STTKLRSD
*HYS*QEDSSIAKCNACGKGEATGSSLPA*FPMG
KS*QNVQGPTRAI*QIC*EMAAFSL*YKDI*SV
ANRS*TVSQKDTNS*ELGTC*IQMVS*GTPGWH
WAAANCCQNIECNWGRNNSAILKNRCQYSTGKIG
KPESAVAGGLQTAVRQKKEARRTKEYLVRISKR
FK*ICFMVGGSR*HC*YPT*TWKRAATKRKA*A
SQVTGGRVAPAPGNSQTIK*NWRTRACKCSHKPR
RAR*T*K*AQADKSPVDKGFQSFT*ETRRN*SS
NKRPWAA*KKA*RP*RAVKSSAAVVISY*ESVG
NL*PTKPRRTI*R*GN*NSSSS*TTGCGRDFV*R
AAFVQGKTSHSASEEEVRRSEL*VEGGKPFTSR
AEGKAA*PSSWTDHYWSLSYSDCYSGDTTCGY*
GNCHLQTRNAIFLDVGGTCSGRFQPGLDRTYRLA
FSA*SSYKITEGDGG*P*GYQRDDHQAEGNNAG
FGTEASPVGRTHYRCPKFEKQDQQSRG*NNHYG
SN*KNSESVG*STRTPSEPEATVE*NVKGFNTMA
GS*GRS*AGLRTGQSQA*VMEGGSLYSRCNPKE
NHRNQAVGQRPPPVADKCRCGK*LGPETSPGLF
CR*YQKSPHDNREYQCLLEKHS*KGE*ARGCFGR
NS*ITATVPPGPGKVSCLAYRS*NNCQCPTGCY
P*GKAPRRLQGSKRADETMARPPR*N*SSHRCL
SQPG*KQPKNPEIPGRFR*CSPVTKTFG*HELQV
E*TSEKVSQH*VPFGSQF*PVEASAPFSAGTSG
VATAER**IKPAGTYWRRLSSSSEAERCT*GLQ
EGIEN*RTCNHEYS*DCTNISDRAAFGRTRETLP
GAQRAAS*GESPECHSASTKAG*GGQY*VGKIE
PALR*LAEKNR*DP*KTPGTSRGHG*AGPQAAP
S*GDQGILAARGRSPH*LSPRSPRESQGTSRRNC
ASEREREPRQ*PCSPAYHFGHSALTV*PQHSGR
PEHQMEASAGGRRGPSQAAA*SPQGLWSSISAL
SFHVCPGSLGESHLAKQSALLYQPRDSNNLLGPS
QNDRALPVFS*PE*CQILSL*DCHETPKTAEGP
LLGSLEPVSCM*CLGPAQPQAK*PAHGYPADY*
LFDHYL*PPGARAQQFGQRPSLRGYVSELAAECL
*YGTNREDPCPVF*NWHHFPV*STFGRQVQIPF
QASGKFNRIL*PAQAGPPSA*FYPNSKTVG*SC
ILWGQ*H*AKCPELLPIC***ARDRSGPLPRLDE
TGTPVHGVAARPAQSGCCRNCQASGQM*HLQRV
SNHWIQVQESKAL*L*HLPKLLFFWSSCKRP*N
ALSHGGILHSDYIRRRCSRLCQGTKKQISNQKVF
CEASPNGLPASADCLRGGQHGNSRYSDQLLASR
FCACLVPSAFTR*YSFTH*TLC*QASRNGKQQW
ILSK**HLS**EHR**TFVNPALLPKFEPGLPPE
PAS*SCPDLDFLRE*GKRGARENPSRS*GRKQE
SASRI*PSKAAART*RPVPTAVPS*NDAHLSPE
SPGC*AHC*GQATASTQRPPGSQDANPGRPQ*TA
GVTVTQAKAAAGATPGRGQSEWHNGVLSFYLST
EVRQQSAYAAPSGWQSNFGLHG*GRSSQSSPGH
KHRVRGGDGATQQLLP*FKRKKYPWKANERGHNV
GSLFHMADDLGRAMESLVSVMTDEEGAE*MFYN
S*FPHGFYNIHTTKRIRQ*EFTRNKSIFL*RVV
VLYCRFQ*FLSLLLFC*QWQVLHVYAIVQKSYKK
TTCKILIAK*LAISLYGTHFGLFKNL*QL*RKI
VN*SVLYKKKLFIKTPKNKTNTHTHTYTHTHKT
LRQRIVLHPFGVISI*NSWLFLFLHIKDKTSSTT
TPNDYYTLLI*ELSAEWGRLEFSFHISICL*VY
KYYSYIDKEIRISID*LFPFFKCSCHILIERNY
F*SVIQAYLLGLEWIFPGAGSQEETTPH*NIVYS
SRCFSF*TTFH*QRK*SKVLDFFKGNM*MNTQD
LLYQSE*SVGWLID*LIDTFSFLLLAMPRFRFN
DASVEINQKVF*PCEHQKVFFNSQAVAGR**GWR
AMDSQPIPVKE*ATL*VKDWMIVHNT*SSL*LQ
LNYYALFSQSKGTGWFGFCCFFRFIVPCGMSF*
MPQDII*NK*TLGKGVKQ*PHHICDTDRYQPRSP
*TVFPSFAFLCE*FHTGL*VSKKEGKLIQMLQK
NPSWWIRQVKYINKQTKIAQKRGEKLKRKS*GL
VGKSFTLSCHFISF*FLNHSFNRYHRVTYNFANL
LPLTSSVISFWRVG*HQV*LAFGEWVLSIINN*
LINIKHGFSCYFYLTLVLGCS**LCTPEFTASP
LVHCVIFFFLYNSFFFLHNFQKKTQSSKVTNYQI
T*RFGFCLAFFSFM*RWTFSLPKDF*NSDLKQG
VTLHPTKKFK*VSFILKSEVNRVHK*FCFNLFV
FLLDTLALE*VCHNI*TKIESFIAAF*A*LIWTL
FRVVFFITTKY*TVNHNVTEA*TSHGMFCHCFQ
VLSSYLSIIIYCVLTPTL*HLRIIFLNFSFTAF
SQHIRLHQIYALLLYYSTALLCISIKHAVML
Frame 1
PGISYCVDSLSVGITHFPPTGLRSGRQLPSEKNE*EKLKCYFF*SC*SLLVSHCF*AYWSNKV*RT
FTRFFLSLP*YTLFKMLWWEEVEDCYEREDVQKK
TFTKWVNAQFSKFGKQHIENLFSDLQDGRRLLD
LLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQ
NNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQ
VKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYP
QVNVINFTTSWSDGLALNALIHSHRPDLFDWNS
VVCQQSATQRLEHAFNIARYQLGIEKLLDPEDVD
TTYPDKKSILMYITSLFQVLPQQVSIEAIQEVE
MLPRPPKVTKEEHFQLHHQMHYSQQITVSLAQG
YERTSSPKPRFKSYAYTQAAYVTTSDPTRSPFPS
QHLEAPEDKSFGSSLMESEVNLDRYQTALEEVL
SWLLSAEDTLQAQGEISNDVEVVKDQFHTHEGY
MMDLTAHQGRVGNILQLGSKLIGTGKLSEDEETE
VQEQMNLLNSRWECLRVASMEKQSNLHRVLMDL
QNQKLKELNDWLTKTEERTRKMEEEPLGPDLED
LKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDE
SSGDHATAALEEQLKVLGDRWANICRWTEDRWV
LLQDILLKWQRLTEEQCLFSAWLSEKEDAVNKI
HTTGFKDQNEMLSSLQKLAVLKADLEKKKQSMGK
LYSLKQDLLSTLKNKSVTQKTEAWLDNFARCWD
NLVQKLEKSTAQISQAVTTTQPSLTQTTVMETV
TTVTTREQILVKHAQEELPPPPPQKKRQITVDSE
IRKRLDVDITELHSWITRSEAVLQSPEFAIFRK
EGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQ
ALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLL
SERLNWLEYQNNIIAFYNQLQQLEQMTTTAENW
LKIQPTTPSEPTAIKSQLKICKDEVNRLSDLQP
QIERLKIQSIALKEKGQGPMFLDADFVAFTNHFK
QVFSDVQAREKELQTIFDTLPPMRYQETMSAIR
TWVQQSETKLSIPQLSVTDYEIMEQRLGELQAL
QSSLQEQQSGLYYLSTTVKEMSKKAPSEISRKYQ
SEFEEIEGRWKKLSSQLVEHCQKLEEQMNKLRK
IQNHIQTLKKWMAEVDVFLKEEWPALGDSEILK
KQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKNEA
EPEFASRLETELKELNTQWDHMCQQVYARKEAL
KGGLEKTVSLQKDLSEMHEWMTQAEEEYLERDF
EYKTPDELQKAVEEMKRAKEEAQQKEAKVKLLTE
SVNSVIAQAPPVAQEALKKELETLTTNYQWLCT
RLNGKCKTLEEVWACWHELLSYLEKANKWLNEV
EFKLKTTENIPGGAEEISEVLDSLENLMRHSEDN
PNQIRILAQTLTDGGVMDELINEELETFNSRWR
ELHEEAVRRQKLLEQSIQSAQETEKSLHLIQES
LTFIDKQLAAYIADKVDAAQMPQEAQKIQSDLTS
HEISLEEMKKHNQGKEAAQRVLSQIDVAQKKLQ
DVSMKFRLFQKPANFEQRLQESKMILDEVKMHL
PALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEV
EMVIKTGRQIVQKKQTENPKELDERVTALKLHY
NELGAKVTERKQQLEKCLKLSRKMRKEMNVLTE
WLAATDMELTKRSAVEGMPSNLDSEVAWGKATQK
EIEKQKVHLKSITEVGEALKTVLGKKETLVEDK
LSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFD
QNVDHITKWIIQADTLLDESEKKKPQQKEDVLKR
LKAELNDIRPKVDSTRDQAANLMANRGDHCRKL
VEPQISELNHRFAAISHRIKTGKASIPLKELEQ
FNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMNED
NEGTVKELLQRGDNLQQRITDERKREEIKIKQQ
LLQTKHNALKDLRSQRRKKALEISHQWYQYKRQ
ADDLLKCLDDIEKKLASLPEPRDERKIKEIDREL
QKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQL
SKRWREIESKFAQFRRLNFAQIHTVREETMMVM
TEDMPLEISYVPSTYLTEITHVSQALLEVEQLLN
APDLCAKDFEDLFKQEESLKNIKDSLQQSSGRI
DIIHSKKTAALQSATPVERVKLQEALSQLDFQW
EKVNKMYKDRQGRFDRSVEKWRRFHYDIKIFNQW
LTEAEQFLRKTQIPENWEHAKYKWYLKELQDGI
GQRQTVVRTLNATGEEIIQQSSKTDASILQEKL
GSLNLRWQEVCKQLSDRKKRLEEQKNILSEFQRD
LNEFVLWLEEADNIASIPLEPGKEQQLKEKLEQ
VKLLVEELPLRQGILKQLNETGGPVLVSAPISP
EEQDKLENKLKQTNLQWIKVSRALPEKQGEIEAQ
IKDLGQLEKKLEDLEEQLNHLLLWLSPIRNQLE
IYNQPNQEGPFDVKETEIAVQAKQPDVEEILSK
GQHLYKEKPATQPVKRKLEDLSSEWKAVNRLLQE
LRAKQPDLAPGLTTIGASPTQTVTLVTQPVVTK
ETAISKLEMPSSLMLEVPALADFNRAWTELTDW
LSLLDQVIKSQRVMVGDLEDINEMIIKQKATMQD
LEQRRPQLEELITAAQNLKNKTSNQEARTIITD
RIERIQNQWDEVQEHLQNRRQQLNEMLKDSTQW
LEAKEEAEQVLGQARAKLESWKEGPYTVDAIQKK
ITETKQLAKDLRQWQTNVDVANDLALKLLRDYS
ADDTRKVHMITENINASWRSIHKRVSEREAALE
ETHRLLQQFPLDLEKFLAWLTEAETTANVLQDAT
RKERLLEDSKGVKELMKQWQDLQGEIEAHTDVY
HNLDENSQKILRSLEGSDDAVLLQRRLDNMNFK
WSELRKKSLNIRSHLEASSDQWKRLHLSLQELLV
WLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFK
RELKTKEPVIMSTLETVRIFLTEQPLEGLEKLY
QEPRELPPEERAQNVTRLLRKQAEEVNTEWEKLN
LHSADWQRKIDETLERLRELQEATDELDLKLRQ
AEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEI
APLKENVSHVNDLARQLTTLGIQLSPYNLSTLED
LNTRWKLLQVAVEDRVRQLHEAHRDFGPASQHF
LSTSVQGPWERAISPNKVPYYINHETQTTCWDH
PKMTELYQSLADLNNVRFSAYRTAMKLRRLQKAL
CLDLLSLSAACDALDQHNLKQNDQPMDILQIIN
CLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNV
YDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLF
KQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVA
SFGGSNIEPSVRSCFQFANNKPEIEAALFLDWM
RLEPQSMVWLPVLHRVAAAETAKHQAKCNICKEC
PIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKM
HYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRY
FAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVD
SAPASSPQLSHDDTHSRIEHYASRLAEMENSNG
SYLNDSISPNESIDDEHLLIQHYCQSLNQDSPL
SQPRSPAQILISLESEERGELERILADLEEENRN
LQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQS
PRDAELIAEAKLLRQHKGRLEARMQILEDHNKQ
LESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQ
RSDSSQPMLLRVVGSQTSDSMGEEDLLSPPQDT
STGLEEVMEQLNNSFPSSRGRNTPGKPMREDTM
*EVFSTWQMIWAERWSP*YQS*QMKKEQNKCFTT
PDSRMVFIIFIQQRGLDSKSLQEINLYFCEG*W
YYTVDFSSF*VCYCFVNNGRFYTSMQLYKKVIR
KLHVKS**LNNLPFLYMERILGCLKIYNSYKERL
*TKVCFIKKSCL*KPLKTKQTHTHTHTHTHTKL
*GSALFCILLA*YPYEIHGFFFFCILKIRLPLP
PHQMTTTHCSFENCQLSGAGLSFHFIYLYVYKYI
NTIVI*IKRYEFL*TDFFHFLNVHVTS**KEIT
SSQSSRLTCLV*NGFFPEPEARRKLHHTKTLST
APDVSHFKQLSTDNESKVKYWIFLKGTCE*IHRT
YYIRVSNRLVG*LID*LIHSASCC*QCHDLDLM
MLQWKSIRRYSDLVNIRRYFLTPKQ*QDDDRAG
GLWIPSPSL*RSRPLFK*RIG*LFIIHKVLCNYN
*IIMPSSHSQKELGGLVFVAFLDLLSHVG*VFK
CHKT*FKINKLWEKV*NSSPITFVILTGINPEA
HELCFHPLHFSASSSTQVCK*VRKKAN*FKCYKK
TLLGGLDRLNI*TNKQKLLKKEEKSSRGKAKDW
*EKALLFHAILFLFDF*IIHSIDTTV*PIILQI
CYL*HQV*LAFGEWADIKCN*LLESGFCPLLIIN
*LTSNTASHAISTSLWFWGVPDNCAHLSSQLHH
LSIALFSFSFIILSFSFIIFKRKPKALR*QITK
LHEDLVFVLHFFPLCDAGPFLYPRIFKTQI*NKG
LLYILLRSLSK*VSF*NQR*IECINNFVLIFLF
FF*TH*LWSESVIIFEQKLRALLLHFKHN*FGH
YFVLCSL*PPSIKL*IIM*LKHKHHMACFVIVFR
Y*VLT*VS*YIVF*HQHCNIYELFF*TSVLLHF
HNISDFTKYMPYYCIIVLLYCVSQ*STQLCY
Frame 2
LASVTVLTHSVLGSLTFPLQDSDLGGNYLRRKTNRKN*SVTFFKAAEVCWFLIVFKPTGAIKFEEL
LPGFFYRCLDIHFSKCFGGKK*RTVMKEKMFKR
KHSQNG*MHNFLSLGSSILRTSSVTYRMGGAS*T
SSKA*QGKNCQKKKDPQEFMP*TMSTRHCGFCR
TIMLI**ILEVLTS*MEIIN*LLV*FGI*SSTG
RSKM**KISWLDCNKPTVKRFS*AGSDNQLVIIH
RLM*STSPPAGLMAWL*MLSSIVIGQTYLTGIV
WFASSQPHNDWNMHSTSPDIN*A*RNYSILKML
IPPIQIRSPS*CTSHHSSKFCLNK*ALKPSRKWK
CCQGHLK*LKKNIFSYIIKCTILNRSRSV*HRD
MRELLPLSLDSRAMPTHRLLMSPPLTLHGAHFL
HSIWKLLKTSHLAVH*WRVK*TWTVIKQL*KKYY
RGFFLLRTHCKHKERFLMMWKW*KTSFILMRGT
*WI*QPIRAGLVIFYNWEVS*LEQENYQKMKKL
KYKSR*IS*IQDGNASG*LAWKNKAIYIEF*WIS
RIRN*KS*MTG*QKQKKEQGKWRKSLLDLILKT
*NAKYNNIRCFKKI*NKNKSGSILSLTWWW*LM
NLVEITQLLLWKNNLRYWEIDGQTSVDGQKTAGF
FYKTSFSNGNVLLKNSAFLVHGFQKKKMQ*TRF
TQLALKIKMKCYQVFKNWPF*KRI*KRKSNPWA
NCIHSNKIFFQH*RISQ*PRRRKHGWITLPGVGI
I*SKNLKRVQHRFHRLSPPLSHH*HRQL*WKQ*
LR*PQGNRSW*SMLKRNFHHHLPKRRGRLLWIL
KLGKGWMLI*LNFTAGLLAQKLCCRVLNLQSFGR
KATSQT*KKKSMP*SEKKLRSSENCKMPADQLR
PWWNRW*MRVLMQIASNKPQNN*TAGGSNSASC
*VRDLTGWSIRTTSSLSIISYNNWSR*QLLLKTG
*KSNPPPHQSQQQLKVS*KFVRMKSTGYQIFNL
KLND*KFKA*P*KRKDKDPCSWMQTLWPLQIIL
SKSFLMCRPERKSYRQFLTLCHQCAIRRP*VPSG
HGSSSQKPNSPYLNLVSPTMKSWSRDSGNCRLY
KVLCKSNKVAYTISAPL*KRCRRKRPLKLAGNI
NQNLKKLRDAGRSSPPSWLSIVKS*RSK*INSEK
FRITYKP*RNGWLKLMFF*RRNGLPLGIQKF*K
SS*NSADF*SVIFRQFSPV*TVSMKVGRR*RMK
QSQSLLRDLRQNSKNLTLSGITCANRSMPERRP*
REVWRKL*ASRKIYQRCTNG*HKLKKSILREIL
NIKLQMNYRKQLKR*RELKKRPNKKKRK*NSLL
SL*IVS*LKLHL*HKRP*KRNLKL*PPTTSGSAL
G*MGNARLWKKFGHVGMSYCHTWRKQTSG*MK*
NLNLKPLKTFLAELRKSLRC*IHLKI*CDIQRI
TQIRFAYWHRP*QMAESWMS*SMRNLRHLILVGG
NYMKRL*GGKSCLNRASSLPRRLKNPYT*SRSP
SHSLTSSWQLILQTRWTQLKCLRKPRKSNLI*Q
VMRSV*KK*RNIIRGRRLPKESCLRLMLHRKNYK
MSP*SFDYSRNQPILSSVYKKVR*F*MK*RCTC
LHWKQRVWNRK*YSHS*IIV*TCIKV*VK*SLK
WKW**RLDVRLYRKSRRKIPKNLMKE*QL*NCII
MSWEQR*QKESNSWRNA*NCPVRCERK*MS*QN
GWQLQIWN*QRDQQLKECLVIWILKLPGERLLK
KRLRNRRCT*RVSQR*ERP*KQFWARRRRWWKIN
SVF*IVTG*LSPPEQKSG*IFCWNTRNTWKLLT
RMWTTSQSGSFRLTHFWMNQRKRNPSKKKTCLS
V*RQN*MTYAQRWTLHVTKQQT*WQTAVTTAGN*
*SPKSQSSTIDLQPFHTELRLERPPFL*RNWSS
LTQIYKNCLNHWRLKFSRG*I*KRKTSIKI*MK
TMRVL*KNCCKEETTYNKESQMRESERK*R*NSS
CYRQNIMLSRI*GLKEEKRL*KFLISGISTRGR
LMIS*NAWMTLKKN*PAYLSPEMKGK*RKLIGN
CRRRKRS*MQCVGKLRACLRMGPQWQWSQLRSSS
ASAGGKLRANLLSFEDSTLHKFTLSVKKR*W**
LKTCLWKFLMCLLLI*LKSLMSHKPY*KWNNFS
MLLTSVLRTLKISLSKRSL*RI*KIVYNKAQVGL
TLFIARRQQHCKVQRLWKG*SYRKLSPSLISNG
KKLTKCTRTDKGDLTDLLRNGGVFIMI*RYLIS
G*QKLNSFSERHKFLRIGNMLNTNGILRNSRMAL
GSGKLLSEH*MQLGKK*FSNPQKQMPVFYRKNW
EA*ICGGRRSANSCQTEKRG*KNKRISCQNFKE
I*MNLFYGWRKQITLLVSHLNLEKSSN*KKSLSK
SSYWWKSCPCAREFSNN*MKLEDPCL*VLP*AQ
KSKINLKISSSRQISSG*RFPELYLRNKEKLKL
K*KTLGSLKKSLKTLKSS*IICCCGYLLLGISWK
FITNQTKKDHLTLRKLK*QFKLNNRMWKRFCLK
GSICTRKNQPLSQ*RGS*KI*ALSGRR*TVYFK
S*GQSSLT*LLD*PLLEPLLLRLLLW*HNLWLLR
KLPSPN*KCHLP*CWRYLLWQISTGLGQNLPTG
FLCLIKL*NHRG*WWVTLRISTR*SSSRRQQCR
IWNRGVPSWKNSLPLPKI*KTRPAIKRLEQSLRI
ELKEFRISGMKYKNTFRTGGNS*MKC*RIQHNG
WKLRKKLSRS*DRPEPSLSHGRRVPIQ*MQSKR
KSQKPSSWPKTSASGRQM*MWQMTWP*NFSGIIL
QMIPEKST**QRISMPLGEAFIKG*VSERLLWK
KLIDYCNSSPWTWKSFLPGLQKLKQLPMSYRML
PVRKGS*KTPRE*KS**NNGKTSKVKLKLTQMFI
TTWMKTAKKS*DPWKVPMMQSCYKDVWIT*TSS
GVNFGKSLSTLGPIWKPVLTSGSVCTFLCRNFW
CGYS*KMMN*AGRHLLEATFQQFRSRTMYIGPSR
GN*KLKNL*S*VLLRLYEYF*QSSLWKD*RNST
RSPESCLLRREPRMSLGFYESRLRRSILSGKN*
TCTPLTGREK*MRPLKDSGNFKRPRMSWTSSCAK
LR*SRDPGSPWAISSLTLSKITSRKSRHFEEKL
RL*KRT*ATSMTLLASLPLWAFSSHRITSALWK
T*TPDGSFCRWPSRTESGSCMKPTGTLVQHLSTF
FPRLSRVPGREPSRQTKCPTISTTRLKQLAGTI
PK*QSSTSL*LT*IMSDSQLIGLP*NSEDCRRP
FAWIS*ACQLHVMPWTSTTSSKMTSPWISCRLLI
V*PLFMTAWSKSTTIWSTSLSAWICV*TGC*MF
MIRDEQGGSVSCLLKLASFPCVKHIWKTSTDTF
SSKWQVQQDFVTSAGWASFCMILSKFQDSWVKLH
PLGAVTLSQVSGAASNLLIISQRSKRPSS*TG*
DWNPSPWCGCPSCTEWLLQKLPSIRPNVTSAKS
VQSLDSGTGV*STLIMTSAKAAFFLVELQKAIKC
TIPWWNIALRLHQEKMFETLPRY*KTNFEPKGI
LRSIPEWATCQCRLS*RGTTWKLPLL*STSGQ*
ILRLPRPLSFHTMILIHALNIMLAG*QKWKTAMD
LI*MIASLLMRA*MMNIC*SSITAKV*TRTPP*
ASLVVLPRS*FP*RVRKEGS*RES*QILRKKTG
ICKQNMTV*SSSTNIKACPHCRPLLK*CPPLPRV
PGMLSSLLRPSYCVNTKAAWKPGCKSWKTTINS
WSHSYTG*GSCWSNPRQRPK*MAQRCPLLLPLY
RGPTAVSLCCSEWLAVKLRTPWVRKIFSVLPRTQ
AQG*RR*WSNSTTPSLVQEEEIPLESQ*ERTQC
RKSFPHGR*FGQSDGVLSISHDR*RRSRINVLQ
LLIPAWFL*YSYNKED*TVRVYKK*IYIFVKGSG
IIL*ISVVSKSVIVLLTMAGFTRLCNCTKKL*E
NYM*NLDS*ITCHFFIWNAFWVV*KFITVIKKD
CKLKCAL*KKVVYKNP*KQNKHTHTHIHTHTQNF
EAAHCFASFWRDIHMKFMAFSFFAY*R*DFLYH
HTK*LLHTAHLRTVS*VGQA*VFISYIYMSISI
*IL*LYR*RDTNFYRLTFSIF*MFMSHPNRKKLL
LVSHPGLPAWSRMDFSRSRKPGGNYTTLKHCLQ
LQMFLILNNFPLTTKVK*SIGFF*REHVNEYTG
LIISE*VIGWLVD*LID*YIQLPAASNATI*I**
CFSGNQSEGILTL*TSEGIF*LPSSSRTMIGLE
GYGFPAHPCEGVGHSLSEGLDDCS*YIKFSVIT
TKLLCPLLTVKRNWVVWFLLLF*IYCPMWDEFLN
ATRHNLK*INFGKRCKTVAPSHL*Y*QVSTQKP
MNCVSILCISLRVVPHRFVSK*ERRQIDSNVTK
KPFLVD*TG*IYKQTNKNCSKKRRKAQEEKLRTG
RKKLYSFMPFYFFLIFKSFIQ*IPPCDL*FCKS
VTSDIKCN*LLESGLTSSVISFWRVGFVHY**L
IN*HQTRLLMLFLPHFGFGVFLIIVHT*VHSFTT
CPLRYFLFPL*FFLFPS*FSKENPKL*GNKLPN
YMKIWFLSCIFFLYVTLDLFFTQGFLKLRFKTR
GYFTSY*EV*VSKFHSKIRGK*SA*IILF*SFCF
SFRHISSGVSLS*YLNKN*ELYCCILSIINLDI
ISCCVLYNHQVLNCKS*CN*SINITWHVLSLFS
GTEFLLEYHNILCFNTNTVTFTNYFFKLQFYCIF
TTYQTSPNICLTIVL*YCFTVYLNKARSYV
Sequence NM_004015
ACTTTCGGGGAGCCCGGCGGCTCTGGGAAGCTCACTCCTCCACTCGTACCCACACTCGACCGCGGAGCCCTTGCAGCCATGAGGGAACAGCTCAAAGGCCACGAGACTCAAACAACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGC
CATGAAACTCCGAAGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGAC
CAGCCCATGGATATCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGG
ATATGTGTCTGAACTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGC
ACATTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATC
CAAATTCCAAGACAGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGA
TCGAAGCGGCCCTCTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCA
TCAGGCCAAATGTAACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTT
TCTGGTCGAGTTGCAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTAC
TAAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCC
CGTTACTCTGATCAACTTCTGGCCAGTAGATTCTGCGCCTGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGC
AGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATCTAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACT
GCCAAAGTTTGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAAT
CCTAGCAGATCTTGAGGAAGAAAACAGGAATCTGCAAGCAGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCT
CCTGAAATGATGCCCACCTCTCCCCAGAGTCCCCGGGATGCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGA
TGCAAATCCTGGAAGACCACAATAAACAGCTGGAGTCACAGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCAC
AACGGTGTCCTCTCCTTCTACCTCTCTACAGAGGTCCGACAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAG
GAAGATCTTCTCAGTCCTCCCCAGGACACAAGCACAGGGTTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCC
CTGGAAAGCCAATGAGAGAGGACACAATGTAGGAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGAT
GAAGAAGGAGCAGAATAAATGTTTTACAACTCCTGATTCCCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAA
ATAAATCTATATTTTTGTGAAGGGTAGTGGTATTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGT
CTATGCAATTGTACAAAAAAGTTATAAGAAAACTACATGTAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTT
AAAAATTTATAACAGTTATAAAGAAAGATTGTAAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACA
CACACACATACACACACACACACAAAACTTTGAGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTT
GCATATTAAAGATAAGACTTCCTCTACCACCACACCAAATGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCA
TTTCATATATCTATATGTCTATAAGTATATAAATACTATAGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTT
CATGTCACATCCTAATAGAAAGAAATTACTTCTAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGA
AACTACACCACACTAAAACATTGTCTACAGCTCCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTT
AAAGGGAACATGTGAATGAATACACAGGACTTATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGC
TGCTAGCAATGCCACGATTTAGATTTAATGATGCTTCAGTGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCA
AGCAGTAGCAGGACGATGATAGGGCTGGAGGGCTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTT
CATAATACATAAAGTTCTCTGTAATTACAACTAAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGAT
TTATTGTCCCATGTGGGATGAGTTTTTAAATGCCACAAGACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTG
TGATACTGACAGGTATCAACCCAGAAGCCCATGAACTGTGTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAA
GAAGGCAAATTGATTCAAATGTTACAAAAAAACCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAG
AAAAGCTCAAGAGGAAAAGCTAAGGACTGGTAGGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATA
CCACCGTGTGACCTATAATTTTGCAAATCTGTTACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGA
GAGTGGGTTTTGTCCATTATTAATAATTAATTAATTAACATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAAT
TGTGCACACCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAAC
CCAAAGCTCTAAGGTAACAAATTACCAAATTACATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAG
GATTTTTAAAACTCAGATTTAAAACAAGGGGTTACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGC
ATAAATAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAGACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGC
TGCATTTTAAGCATAATTAATTTGGACATTATTTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACA
TCACATGGCATGTTTTGTCATTGTTTTCAGGTACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTA
TTTTTTTAAACTTCAGTTTTACTGCATTTTCACAACATATCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTC
AATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
TFGEPGGSGKLTPPLVPTLDRGALAAMREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTA
MKLRRLQKALCLDLLSLSAACDALDQHNLKQND
QPMDILQIINCLTTIYDRLEQEHNNLVNVPLCV
DMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKA
HLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSI
QIPRQLGEVASFGGSNIEPSVRSCFQFANNKPE
IEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKH
QAKCNICKECPIIGFRYRSLKHFNYDICQSCFF
SGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKV
LKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETP
VTLINFWPVDSAPASSPQLSHDDTHSRIEHYAS
RLAEMENSNGSYLNDSISPNESIDDEHLLIQHY
CQSLNQDSPLSQPRSPAQILISLESEERGELERI
LADLEEENRNLQAEYDRLKQQHEHKGLSPLPSP
PEMMPTSPQSPRDAELIAEAKLLRQHKGRLEAR
MQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGT
TVSSPSTSLQRSDSSQPMLLRVVGSQTSDSMGE
EDLLSPPQDTSTGLEEVMEQLNNSFPSSRGRNT
PGKPMREDTM*EVFSTWQMIWAERWSP*YQS*QM
KKEQNKCFTTPDSRMVFIIFIQQRGLDSKSLQE
INLYFCEG*WYYTVDFSSF*VCYCFVNNGRFYT
SMQLYKKVIRKLHVKS**LNNLPFLYMERILGCL
KIYNSYKERL*TKVCFIKKSCL*KPLKTKQTHT
HTHTHTHTKL*GSALFCILLA*YPYEIHGFFFF
CILKIRLPLPPHQMTTTHCSFENCQLSGAGLSFH
FIYLYVYKYINTIVI*IKRYEFL*TDFFHFLNV
HVTS**KEITSSQSSRLTCLV*NGFFPEPEARR
KLHHTKTLSTAPDVSHFKQLSTDNESKVKYWIFL
KGTCE*IHRTYYIRVSNRLVG*LID*LIHSASC
C*QCHDLDLMMLQWKSIRRYSDLVNIRRYFLTP
KQ*QDDDRAGGLWIPSPSL*RSRPLFK*RIG*LF
IIHKVLCNYN*IIMPSSHSQKELGGLVFVAFLD
LLSHVG*VFKCHKT*FKINKLWEKV*NSSPITF
VILTGINPEAHELCFHPLHFSASSSTQVCK*VRK
KAN*FKCYKKTLLGGLDRLNI*TNKQKLLKKEE
KSSRGKAKDW*EKALLFHAILFLFDF*IIHSID
TTV*PIILQICYL*HQV*LAFGEWADIKCN*LLE
SGFCPLLIIN*LTSNTASHAISTSLWFWGVPDN
CAHLSSQLHHLSIALFSFSFIILSFSFIIFKRK
PKALR*QITKLHEDLVFVLHFFPLCDAGPFLYPR
IFKTQI*NKGLLYILLRSLSK*VSF*NQR*IEC
INNFVLIFLFFF*TH*LWSESVIIFEQKLRALL
LHFKHN*FGHYFVLCSL*PPSIKL*IIM*LKHKH
HMACFVIVFRY*VLT*VS*YIVF*HQHCNIYEL
FF*TSVLLHFHNISDFTKYMPYYCIIVLLYCVS
Q*STQLCY
Frame 1
LSGSPAALGSSLLHSYPHSTAEPLQP*GNSSKATRLKQLAGTIPK*QSSTSL*LT*IMSDSQLIGL
P*NSEDCRRPFAWIS*ACQLHVMPWTSTTSSKMT
SPWISCRLLIV*PLFMTAWSKSTTIWSTSLSAW
ICV*TGC*MFMIRDEQGGSVSCLLKLASFPCVK
HIWKTSTDTFSSKWQVQQDFVTSAGWASFCMILS
KFQDSWVKLHPLGAVTLSQVSGAASNLLIISQR
SKRPSS*TG*DWNPSPWCGCPSCTEWLLQKLPS
IRPNVTSAKSVQSLDSGTGV*STLIMTSAKAAFF
LVELQKAIKCTIPWWNIALRLHQEKMFETLPRY
*KTNFEPKGILRSIPEWATCQCRLS*RGTTWKL
PLL*STSGQ*ILRLPRPLSFHTMILIHALNIMLA
G*QKWKTAMDLI*MIASLLMRA*MMNIC*SSIT
AKV*TRTPP*ASLVVLPRS*FP*RVRKEGS*RE
S*QILRKKTGICKQNMTV*SSSTNIKACPHCRPL
LK*CPPLPRVPGMLSSLLRPSYCVNTKAAWKPG
CKSWKTTINSWSHSYTG*GSCWSNPRQRPK*MA
QRCPLLLPLYRGPTAVSLCCSEWLAVKLRTPWVR
KIFSVLPRTQAQG*RR*WSNSTTPSLVQEEEIP
LESQ*ERTQCRKSFPHGR*FGQSDGVLSISHDR
*RRSRINVLQLLIPAWFL*YSYNKED*TVRVYKK
*IYIFVKGSGIIL*ISVVSKSVIVLLTMAGFTR
LCNCTKKL*ENYM*NLDS*ITCHFFIWNAFWVV
*KFITVIKKDCKLKCAL*KKVVYKNP*KQNKHTH
THIHTHTQNFEAAHCFASFWRDIHMKFMAFSFF
AY*R*DFLYHHTK*LLHTAHLRTVS*VGQA*VF
ISYIYMSISI*IL*LYR*RDTNFYRLTFSIF*MF
MSHPNRKKLLLVSHPGLPAWSRMDFSRSRKPGG
NYTTLKHCLQLQMFLILNNFPLTTKVK*SIGFF
*REHVNEYTGLIISE*VIGWLVD*LID*YIQLPA
ASNATI*I**CFSGNQSEGILTL*TSEGIF*LP
SSSRTMIGLEGYGFPAHPCEGVGHSLSEGLDDC
S*YIKFSVITTKLLCPLLTVKRNWVVWFLLLF*I
YCPMWDEFLNATRHNLK*INFGKRCKTVAPSHL
*Y*QVSTQKPMNCVSILCISLRVVPHRFVSK*E
RRQIDSNVTKKPFLVD*TG*IYKQTNKNCSKKRR
KAQEEKLRTGRKKLYSFMPFYFFLIFKSFIQ*I
PPCDL*FCKSVTSDIKCN*LLESGLTSSVISFW
RVGFVHY**LIN*HQTRLLMLFLPHFGFGVFLII
VHT*VHSFTTCPLRYFLFPL*FFLFPS*FSKEN
PKL*GNKLPNYMKIWFLSCIFFLYVTLDLFFTQ
GFLKLRFKTRGYFTSY*EV*VSKFHSKIRGK*SA
*IILF*SFCFSFRHISSGVSLS*YLNKN*ELYC
CILSIINLDIISCCVLYNHQVLNCKS*CN*SIN
ITWHVLSLFSGTEFLLEYHNILCFNTNTVTFTNY
FFKLQFYCIFTTYQTSPNICLTIVL*YCFTVYL
NKARSYV
Frame 2
FRGARRLWEAHSSTRTHTRPRSPCSHEGTAQRPRDSNNLLGPSQNDRALPVFS*PE*CQILSL*DC
HETPKTAEGPLLGSLEPVSCM*CLGPAQPQAK*
PAHGYPADY*LFDHYL*PPGARAQQFGQRPSLRG
YVSELAAECL*YGTNREDPCPVF*NWHHFPV*S
TFGRQVQIPFQASGKFNRIL*PAQAGPPSA*FY
PNSKTVG*SCILWGQ*H*AKCPELLPIC***ARD
RSGPLPRLDETGTPVHGVAARPAQSGCCRNCQA
SGQM*HLQRVSNHWIQVQESKAL*L*HLPKLLF
FWSSCKRP*NALSHGGILHSDYIRRRCSRLCQGT
KKQISNQKVFCEASPNGLPASADCLRGGQHGNS
RYSDQLLASRFCACLVPSAFTR*YSFTH*TLC*
QASRNGKQQWILSK**HLS**EHR**TFVNPALL
PKFEPGLPPEPAS*SCPDLDFLRE*GKRGAREN
PSRS*GRKQESASRI*PSKAAART*RPVPTAVP
S*NDAHLSPESPGC*AHC*GQATASTQRPPGSQD
ANPGRPQ*TAGVTVTQAKAAAGATPGRGQSEWH
NGVLSFYLSTEVRQQSAYAAPSGWQSNFGLHG*
GRSSQSSPGHKHRVRGGDGATQQLLP*FKRKKYP
WKANERGHNVGSLFHMADDLGRAMESLVSVMTD
EEGAE*MFYNS*FPHGFYNIHTTKRIRQ*EFTR
NKSIFL*RVVVLYCRFQ*FLSLLLFC*QWQVLHV
YAIVQKSYKKTTCKILIAK*LAISLYGTHFGLF
KNL*QL*RKIVN*SVLYKKKLFIKTPKNKTNTH
THTYTHTHKTLRQRIVLHPFGVISI*NSWLFLFL
HIKDKTSSTTTPNDYYTLLI*ELSAEWGRLEFS
FHISICL*VYKYYSYIDKEIRISID*LFPFFKC
SCHILIERNYF*SVIQAYLLGLEWIFPGAGSQEE
TTPH*NIVYSSRCFSF*TTFH*QRK*SKVLDFF
KGNM*MNTQDLLYQSE*SVGWLID*LIDTFSFL
LLAMPRFRFNDASVEINQKVF*PCEHQKVFFNSQ
AVAGR**GWRAMDSQPIPVKE*ATL*VKDWMIV
HNT*SSL*LQLNYYALFSQSKGTGWFGFCCFFR
FIVPCGMSF*MPQDII*NK*TLGKGVKQ*PHHIC
DTDRYQPRSP*TVFPSFAFLCE*FHTGL*VSKK
EGKLIQMLQKNPSWWIRQVKYINKQTKIAQKRG
EKLKRKS*GLVGKSFTLSCHFISF*FLNHSFNRY
HRVTYNFANLLPLTSSVISFWRVG*HQV*LAFG
EWVLSIINN*LINIKHGFSCYFYLTLVLGCS**
LCTPEFTASPLVHCVIFFFLYNSFFFLHNFQKKT
QSSKVTNYQIT*RFGFCLAFFSFM*RWTFSLPK
DF*NSDLKQGVTLHPTKKFK*VSFILKSEVNRV
HK*FCFNLFVFLLDTLALE*VCHNI*TKIESFIA
AF*A*LIWTLFRVVFFITTKY*TVNHNVTEA*T
SHGMFCHCFQVLSSYLSIIIYCVLTPTL*HLRI
IFLNFSFTAFSQHIRLHQIYALLLYYSTALLCIS
IKHAVML
Sequence NM_004012
TAATGAGATCAGGAGGAACATTCGACCTGAGAAAGACAGATTGCAATGACTGAGATGATTTTGCTAATTTTTTTTCCAGCCTATTTCCTTAATGTACGTGATATTGTGATTGATTTTCATGCAGAGATCCCTGATCCTATAGTTTTGTTTGCTATTTATTTTTCTCCTTCACATTTTTTTTCTATCAACAGAGCTGAATG
AGTGCCAGGAAGCTGCGAAATCTGTCTTACAAAAAGGCTGTAAGGAGGCAAAAGTTGCTTGAACAGAGCATCCAGTCTGCCCAGGAGACTGAAAAATCCT
TACACTTAATCCAGGAGTCCCTCACATTCATTGACAAGCAGTTGGCAGCTTATATTGCAGACAAGGTGGACGCAGCTCAAATGCCTCAGGAAGCCCAGAA
AATCCAATCTGATTTGACAAGTCATGAGATCAGTTTAGAAGAAATGAAGAAACATAATCAGGGGAAGGAGGCTGCCCAAAGAGTCCTGTCTCAGATTGAT
GTTGCACAGAAAAAATTACAAGATGTCTCCATGAAGTTTCGATTATTCCAGAAACCAGCCAATTTTGAGCAGCGTCTACAAGAAAGTAAGATGATTTTAG
ATGAAGTGAAGATGCACTTGCCTGCATTGGAAACAAAGAGTGTGGAACAGGAAGTAGTACAGTCACAGCTAAATCATTGTGTGAACTTGTATAAAAGTCT
GAGTGAAGTGAAGTCTGAAGTGGAAATGGTGATAAAGACTGGACGTCAGATTGTACAGAAAAAGCAGACGGAAAATCCCAAAGAACTTGATGAAAGAGTA
ACAGCTTTGAAATTGCATTATAATGAGCTGGGAGCAAAGGTAACAGAAAGAAAGCAACAGTTGGAGAAATGCTTGAAATTGTCCCGTAAGATGCGAAAGG
AAATGAATGTCTTGACAGAATGGCTGGCAGCTACAGATATGGAATTGACAAAGAGATCAGCAGTTGAAGGAATGCCTAGTAATTTGGATTCTGAAGTTGC
CTGGGGAAAGGCTACTCAAAAAGAGATTGAGAAACAGAAGGTGCACCTGAAGAGTATCACAGAGGTAGGAGAGGCCTTGAAAACAGTTTTGGGCAAGAAG
GAGACGTTGGTGGAAGATAAACTCAGTCTTCTGAATAGTAACTGGATAGCTGTCACCTCCCGAGCAGAAGAGTGGTTAAATCTTTTGTTGGAATACCAGA
AACACATGGAAACTTTTGACCAGAATGTGGACCACATCACAAAGTGGATCATTCAGGCTGACACACTTTTGGATGAATCAGAGAAAAAGAAACCCCAGCA
AAAAGAAGACGTGCTTAAGCGTTTAAAGGCAGAACTGAATGACATACGCCCAAAGGTGGACTCTACACGTGACCAAGCAGCAAACTTGATGGCAAACCGC
GGTGACCACTGCAGGAAATTAGTAGAGCCCCAAATCTCAGAGCTCAACCATCGATTTGCAGCCATTTCACACAGAATTAAGACTGGAAAGGCCTCCATTC
CTTTGAAGGAATTGGAGCAGTTTAACTCAGATATACAAAAATTGCTTGAACCACTGGAGGCTGAAATTCAGCAGGGGGTGAATCTGAAAGAGGAAGACTT
CAATAAAGATATGAATGAAGACAATGAGGGTACTGTAAAAGAATTGTTGCAAAGAGGAGACAACTTACAACAAAGAATCACAGATGAGAGAAAGCGAGAG
GAAATAAAGATAAAACAGCAGCTGTTACAGACAAAACATAATGCTCTCAAGGATTTGAGGTCTCAAAGAAGAAAAAAGGCTCTAGAAATTTCTCATCAGT
GGTATCAGTACAAGAGGCAGGCTGATGATCTCCTGAAATGCTTGGATGACATTGAAAAAAAATTAGCCAGCCTACCTGAGCCCAGAGATGAAAGGAAAAT
AAAGGAAATTGATCGGGAATTGCAGAAGAAGAAAGAGGAGCTGAATGCAGTGCGTAGGCAAGCTGAGGGCTTGTCTGAGGATGGGGCCGCAATGGCAGTG
GAGCCAACTCAGATCCAGCTCAGCAAGCGCTGGCGGGAAATTGAGAGCAAATTTGCTCAGTTTCGAAGACTCAACTTTGCACAAATTCACACTGTCCGTG
AAGAAACGATGATGGTGATGACTGAAGACATGCCTTTGGAAATTTCTTATGTGCCTTCTACTTATTTGACTGAAATCACTCATGTCTCACAAGCCCTATT
AGAAGTGGAACAACTTCTCAATGCTCCTGACCTCTGTGCTAAGGACTTTGAAGATCTCTTTAAGCAAGAGGAGTCTCTGAAGAATATAAAAGATAGTCTA
CAACAAAGCTCAGGTCGGATTGACATTATTCATAGCAAGAAGACAGCAGCATTGCAAAGTGCAACGCCTGTGGAAAGGGTGAAGCTACAGGAAGCTCTCT
CCCAGCTTGATTTCCAATGGGAAAAAGTTAACAAAATGTACAAGGACCGACAAGGGCGATTTGACAGATCTGTTGAGAAATGGCGGCGTTTTCATTATGA
TATAAAGATATTTAATCAGTGGCTAACAGAAGCTGAACAGTTTCTCAGAAAGACACAAATTCCTGAGAATTGGGAACATGCTAAATACAAATGGTATCTT
AAGGAACTCCAGGATGGCATTGGGCAGCGGCAAACTGTTGTCAGAACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGATGCCA
GTATTCTACAGGAAAAATTGGGAAGCCTGAATCTGCGGTGGCAGGAGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGGCTAGAAGAACAAAAGAATAT
CTTGTCAGAATTTCAAAGAGATTTAAATGAATTTGTTTTATGGTTGGAGGAAGCAGATAACATTGCTAGTATCCCACTTGAACCTGGAAAAGAGCAGCAA
CTAAAAGAAAAGCTTGAGCAAGTCAAGTTACTGGTGGAAGAGTTGCCCCTGCGCCAGGGAATTCTCAAACAATTAAATGAAACTGGAGGACCCGTGCTTG
TAAGTGCTCCCATAAGCCCAGAAGAGCAAGATAAACTTGAAAATAAGCTCAAGCAGACAAATCTCCAGTGGATAAAGGTTTCCAGAGCTTTACCTGAGAA
ACAAGGAGAAATTGAAGCTCAAATAAAAGACCTTGGGCAGCTTGAAAAAAAGCTTGAAGACCTTGAAGAGCAGTTAAATCATCTGCTGCTGTGGTTATCT
CCTATTAGGAATCAGTTGGAAATTTATAACCAACCAAACCAAGAAGGACCATTTGACGTTAAGGAAACTGAAATAGCAGTTCAAGCTAAACAACCGGATG
TGGAAGAGATTTTGTCTAAAGGGCAGCATTTGTACAAGGAAAAACCAGCCACTCAGCCAGTGAAGAGGAAGTTAGAAGATCTGAGCTCTGAGTGGAAGGC
GGTAAACCGTTTACTTCAAGAGCTGAGGGCAAAGCAGCCTGACCTAGCTCCTGGACTGACCACTATTGGAGCCTCTCCTACTCAGACTGTTACTCTGGTG
ACACAACCTGTGGTTACTAAGGAAACTGCCATCTCCAAACTAGAAATGCCATCTTCCTTGATGTTGGAGGTACCTGCTCTGGCAGATTTCAACCGGGCTT
GGACAGAACTTACCGACTGGCTTTCTCTGCTTGATCAAGTTATAAAATCACAGAGGGTGATGGTGGGTGACCTTGAGGATATCAACGAGATGATCATCAA
GCAGAAGGCAACAATGCAGGATTTGGAACAGAGGCGTCCCCAGTTGGAAGAACTCATTACCGCTGCCCAAAATTTGAAAAACAAGACCAGCAATCAAGAG
GCTAGAACAATCATTACGGATCGAATTGAAAGAATTCAGAATCAGTGGGATGAAGTACAAGAACACCTTCAGAACCGGAGGCAACAGTTGAATGAAATGT
TAAAGGATTCAACACAATGGCTGGAAGCTAAGGAAGAAGCTGAGCAGGTCTTAGGACAGGCCAGAGCCAAGCTTGAGTCATGGAAGGAGGGTCCCTATAC
AGTAGATGCAATCCAAAAGAAAATCACAGAAACCAAGCAGTTGGCCAAAGACCTCCGCCAGTGGCAGACAAATGTAGATGTGGCAAATGACTTGGCCCTG
AAACTTCTCCGGGATTATTCTGCAGATGATACCAGAAAAGTCCACATGATAACAGAGAATATCAATGCCTCTTGGAGAAGCATTCATAAAAGGGTGAGTG
AGCGAGAGGCTGCTTTGGAAGAAACTCATAGATTACTGCAACAGTTCCCCCTGGACCTGGAAAAGTTTCTTGCCTGGCTTACAGAAGCTGAAACAACTGC
CAATGTCCTACAGGATGCTACCCGTAAGGAAAGGCTCCTAGAAGACTCCAAGGGAGTAAAAGAGCTGATGAAACAATGGCAAGACCTCCAAGGTGAAATT
GAAGCTCACACAGATGTTTATCACAACCTGGATGAAAACAGCCAAAAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTACAAAGACGTT
TGGATAACATGAACTTCAAGTGGAGTGAACTTCGGAAAAAGTCTCTCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCGTCTGCACCT
TTCTCTGCAGGAACTTCTGGTGTGGCTACAGCTGAAAGATGATGAATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAGAAGCAGAAC
GATGTACATAGGGCCTTCAAGAGGGAATTGAAAACTAAAGAACCTGTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGCAGCCTTTGG
AAGGACTAGAGAAACTCTACCAGGAGCCCAGAGAGCTGCCTCCTGAGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGAGGAGGTCAA
TACTGAGTGGGAAAAATTGAACCTGCACTCCGCTGACTGGCAGAGAAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCCACGGATGAG
CTGGACCTCAAGCTGCGCCAAGCTGAGGTGATCAAGGGATCCTGGCAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCGAGAAAGTCA
AGGCACTTCGAGGAGAAATTGCGCCTCTGAAAGAGAACGTGAGCCACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCTCTCACCGTA
TAACCTCAGCACTCTGGAAGACCTGAACACCAGATGGAAGCTTCTGCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCACAGGGACTTT
GGTCCAGCATCTCAGCACTTTCTTTCCACGTCTGTCCAGGGTCCCTGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACCACGAGACTC
AAACAACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGCCATGAAACT
CCGAAGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGACCAGCCCATG
GATATCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGGATATGTGTC
TGAACTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGCACATTTGGA
AGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATCCAAATTCCA
AGACAGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGATCGAAGCGG
CCCTCTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCATCAGGCCAA
ATGTAACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGTCGA
GTTGCAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTAAAAAACA
AATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCCCGTTACTCT
GATCAACTTCTGGCCAGTAGATTCTGCGCCTGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCA
GAAATGGAAAACAGCAATGGATCTTATCTAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTT
TGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGA
TCTTGAGGAAGAAAACAGGAATCTGCAAGCAGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATG
ATGCCCACCTCTCCCCAGAGTCCCCGGGATGCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCC
TGGAAGACCACAATAAACAGCTGGAGTCACAGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTC
CTCTCCTTCTACCTCTCTACAGAGGTCCGACAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTT
CTCAGTCCTCCCCAGGACACAAGCACAGGGTTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCCCTGGAAAGC
CAATGAGAGAGGACACAATGTAGGAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGA
GCAGAATAAATGTTTTACAACTCCTGATTCCCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTA
TATTTTTGTGAAGGGTAGTGGTATTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAAT
TGTACAAAAAAGTTATAAGAAAACTACATGTAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTA
TAACAGTTATAAAGAAAGATTGTAAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACAT
ACACACACACACACAAAACTTTGAGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAA
AGATAAGACTTCCTCTACCACCACACCAAATGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATA
TCTATATGTCTATAAGTATATAAATACTATAGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACA
TCCTAATAGAAAGAAATTACTTCTAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACC
ACACTAAAACATTGTCTACAGCTCCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAAC
ATGTGAATGAATACACAGGACTTATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAA
TGCCACGATTTAGATTTAATGATGCTTCAGTGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGC
AGGACGATGATAGGGCTGGAGGGCTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACA
TAAAGTTCTCTGTAATTACAACTAAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCC
CATGTGGGATGAGTTTTTAAATGCCACAAGACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGA
CAGGTATCAACCCAGAAGCCCATGAACTGTGTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAA
TTGATTCAAATGTTACAAAAAAACCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCA
AGAGGAAAAGCTAAGGACTGGTAGGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACCGTGT
GACCTATAATTTTGCAAATCTGTTACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTT
TTGTCCATTATTAATAATTAATTAATTAACATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACAC
CTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTC
TAAGGTAACAAATTACCAAATTACATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAA
AACTCAGATTTAAAACAAGGGGTTACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAAT
TTTGTTTTAATCTTTTTGTTTTTCTTTTAGACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTA
AGCATAATTAATTTGGACATTATTTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGC
ATGTTTTGTCATTGTTTTCAGGTACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTTTTAA
ACTTCAGTTTTACTGCATTTTCACAACATATCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCA
CGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
**DQEEHST*ERQIAMTEMILLIFFPAYFLNVRDIVIDFHAEIPDPIVLFAIYFSPSHFFSINRAE*
VPGSCEICLTKRL*GGKSCLNRASSLPRRLKNP
YT*SRSPSHSLTSSWQLILQTRWTQLKCLRKPR
KSNLI*QVMRSV*KK*RNIIRGRRLPKESCLRLM
LHRKNYKMSP*SFDYSRNQPILSSVYKKVR*F*
MK*RCTCLHWKQRVWNRK*YSHS*IIV*TCIKV
*VK*SLKWKW**RLDVRLYRKSRRKIPKNLMKE*
QL*NCIIMSWEQR*QKESNSWRNA*NCPVRCER
K*MS*QNGWQLQIWN*QRDQQLKECLVIWILKL
PGERLLKKRLRNRRCT*RVSQR*ERP*KQFWARR
RRWWKINSVF*IVTG*LSPPEQKSG*IFCWNTR
NTWKLLTRMWTTSQSGSFRLTHFWMNQRKRNPS
KKKTCLSV*RQN*MTYAQRWTLHVTKQQT*WQTA
VTTAGN**SPKSQSSTIDLQPFHTELRLERPPF
L*RNWSSLTQIYKNCLNHWRLKFSRG*I*KRKT
SIKI*MKTMRVL*KNCCKEETTYNKESQMRESER
K*R*NSSCYRQNIMLSRI*GLKEEKRL*KFLIS
GISTRGRLMIS*NAWMTLKKN*PAYLSPEMKGK
*RKLIGNCRRRKRS*MQCVGKLRACLRMGPQWQW
SQLRSSSASAGGKLRANLLSFEDSTLHKFTLSV
KKR*W**LKTCLWKFLMCLLLI*LKSLMSHKPY
*KWNNFSMLLTSVLRTLKISLSKRSL*RI*KIVY
NKAQVGLTLFIARRQQHCKVQRLWKG*SYRKLS
PSLISNGKKLTKCTRTDKGDLTDLLRNGGVFIM
I*RYLISG*QKLNSFSERHKFLRIGNMLNTNGIL
RNSRMALGSGKLLSEH*MQLGKK*FSNPQKQMP
VFYRKNWEA*ICGGRRSANSCQTEKRG*KNKRI
SCQNFKEI*MNLFYGWRKQITLLVSHLNLEKSSN
*KKSLSKSSYWWKSCPCAREFSNN*MKLEDPCL
*VLP*AQKSKINLKISSSRQISSG*RFPELYLR
NKEKLKLK*KTLGSLKKSLKTLKSS*IICCCGYL
LLGISWKFITNQTKKDHLTLRKLK*QFKLNNRM
WKRFCLKGSICTRKNQPLSQ*RGS*KI*ALSGR
R*TVYFKS*GQSSLT*LLD*PLLEPLLLRLLLW*
HNLWLLRKLPSPN*KCHLP*CWRYLLWQISTGL
GQNLPTGFLCLIKL*NHRG*WWVTLRISTR*SS
SRRQQCRIWNRGVPSWKNSLPLPKI*KTRPAIKR
LEQSLRIELKEFRISGMKYKNTFRTGGNS*MKC
*RIQHNGWKLRKKLSRS*DRPEPSLSHGRRVPI
Q*MQSKRKSQKPSSWPKTSASGRQM*MWQMTWP*
NFSGIILQMIPEKST**QRISMPLGEAFIKG*V
SERLLWKKLIDYCNSSPWTWKSFLPGLQKLKQL
PMSYRMLPVRKGS*KTPRE*KS**NNGKTSKVKL
KLTQMFITTWMKTAKKS*DPWKVPMMQSCYKDV
WIT*TSSGVNFGKSLSTLGPIWKPVLTSGSVCT
FLCRNFWCGYS*KMMN*AGRHLLEATFQQFRSRT
MYIGPSRGN*KLKNL*S*VLLRLYEYF*QSSLW
KD*RNSTRSPESCLLRREPRMSLGFYESRLRRS
ILSGKN*TCTPLTGREK*MRPLKDSGNFKRPRMS
WTSSCAKLR*SRDPGSPWAISSLTLSKITSRKS
RHFEEKLRL*KRT*ATSMTLLASLPLWAFSSHR
ITSALWKT*TPDGSFCRWPSRTESGSCMKPTGTL
VQHLSTFFPRLSRVPGREPSRQTKCPTISTTRL
KQLAGTIPK*QSSTSL*LT*IMSDSQLIGLP*N
SEDCRRPFAWIS*ACQLHVMPWTSTTSSKMTSPW
ISCRLLIV*PLFMTAWSKSTTIWSTSLSAWICV
*TGC*MFMIRDEQGGSVSCLLKLASFPCVKHIW
KTSTDTFSSKWQVQQDFVTSAGWASFCMILSKFQ
DSWVKLHPLGAVTLSQVSGAASNLLIISQRSKR
PSS*TG*DWNPSPWCGCPSCTEWLLQKLPSIRP
NVTSAKSVQSLDSGTGV*STLIMTSAKAAFFLVE
LQKAIKCTIPWWNIALRLHQEKMFETLPRY*KT
NFEPKGILRSIPEWATCQCRLS*RGTTWKLPLL
*STSGQ*ILRLPRPLSFHTMILIHALNIMLAG*Q
KWKTAMDLI*MIASLLMRA*MMNIC*SSITAKV
*TRTPP*ASLVVLPRS*FP*RVRKEGS*RES*Q
ILRKKTGICKQNMTV*SSSTNIKACPHCRPLLK*
CPPLPRVPGMLSSLLRPSYCVNTKAAWKPGCKS
WKTTINSWSHSYTG*GSCWSNPRQRPK*MAQRC
PLLLPLYRGPTAVSLCCSEWLAVKLRTPWVRKIF
SVLPRTQAQG*RR*WSNSTTPSLVQEEEIPLES
Q*ERTQCRKSFPHGR*FGQSDGVLSISHDR*RR
SRINVLQLLIPAWFL*YSYNKED*TVRVYKK*IY
IFVKGSGIIL*ISVVSKSVIVLLTMAGFTRLCN
CTKKL*ENYM*NLDS*ITCHFFIWNAFWVV*KF
ITVIKKDCKLKCAL*KKVVYKNP*KQNKHTHTHI
HTHTQNFEAAHCFASFWRDIHMKFMAFSFFAY*
R*DFLYHHTK*LLHTAHLRTVS*VGQA*VFISY
IYMSISI*IL*LYR*RDTNFYRLTFSIF*MFMSH
PNRKKLLLVSHPGLPAWSRMDFSRSRKPGGNYT
TLKHCLQLQMFLILNNFPLTTKVK*SIGFF*RE
HVNEYTGLIISE*VIGWLVD*LID*YIQLPAASN
ATI*I**CFSGNQSEGILTL*TSEGIF*LPSSS
RTMIGLEGYGFPAHPCEGVGHSLSEGLDDCS*Y
IKFSVITTKLLCPLLTVKRNWVVWFLLLF*IYCP
MWDEFLNATRHNLK*INFGKRCKTVAPSHL*Y*
QVSTQKPMNCVSILCISLRVVPHRFVSK*ERRQ
IDSNVTKKPFLVD*TG*IYKQTNKNCSKKRRKAQ
EEKLRTGRKKLYSFMPFYFFLIFKSFIQ*IPPC
DL*FCKSVTSDIKCN*LLESGLTSSVISFWRVG
FVHY**LIN*HQTRLLMLFLPHFGFGVFLIIVHT
*VHSFTTCPLRYFLFPL*FFLFPS*FSKENPKL
*GNKLPNYMKIWFLSCIFFLYVTLDLFFTQGFL
KLRFKTRGYFTSY*EV*VSKFHSKIRGK*SA*II
LF*SFCFSFRHISSGVSLS*YLNKN*ELYCCIL
SIINLDIISCCVLYNHQVLNCKS*CN*SINITW
HVLSLFSGTEFLLEYHNILCFNTNTVTFTNYFFK
LQFYCIFTTYQTSPNICLTIVL*YCFTVYLNKA
RSYV
Frame 1
NEIRRNIRPEKDRLQ*LR*FC*FFFQPISLMYVIL*LIFMQRSLIL*FCLLFIFLLHIFFLSTELN
ECQEAAKSVLQKGCKEAKVA*TEHPVCPGD*KIL
TLNPGVPHIH*QAVGSLYCRQGGRSSNASGSPE
NPI*FDKS*DQFRRNEET*SGEGGCPKSPVSD*
CCTEKITRCLHEVSIIPETSQF*AASTRK*DDFR
*SEDALACIGNKECGTGSSTVTAKSLCELV*KS
E*SEV*SGNGDKDWTSDCTEKADGKSQRT**KS
NSFEIAL**AGSKGNRKKATVGEMLEIVP*DAKG
NECLDRMAGSYRYGIDKEISS*RNA**FGF*SC
LGKGYSKRD*ETEGAPEEYHRGRRGLENSFGQE
GDVGGR*TQSSE**LDSCHLPSRRVVKSFVGIPE
THGNF*PECGPHHKVDHSG*HTFG*IREKETPA
KRRRA*AFKGRTE*HTPKGGLYT*PSSKLDGKP
R*PLQEISRAPNLRAQPSICSHFTQN*DWKGLHS
FEGIGAV*LRYTKIA*TTGG*NSAGGESERGRL
Q*RYE*RQ*GYCKRIVAKRRQLTTKNHR*EKAR
GNKDKTAAVTDKT*CSQGFEVSKKKKGSRNFSSV
VSVQEAG**SPEMLG*H*KKISQPT*AQR*KEN
KGN*SGIAEEERGAECSA*AS*GLV*GWGRNGS
GANSDPAQQALAGN*EQICSVSKTQLCTNSHCP*
RNDDGDD*RHAFGNFLCAFYLFD*NHSCLTSPI
RSGTTSQCS*PLC*GL*RSL*ARGVSEEYKR*S
TTKLRSD*HYS*QEDSSIAKCNACGKGEATGSSL
PA*FPMGKS*QNVQGPTRAI*QIC*EMAAFSL*
YKDI*SVANRS*TVSQKDTNS*ELGTC*IQMVS
*GTPGWHWAAANCCQNIECNWGRNNSAILKNRCQ
YSTGKIGKPESAVAGGLQTAVRQKKEARRTKEY
LVRISKRFK*ICFMVGGSR*HC*YPT*TWKRAA
TKRKA*ASQVTGGRVAPAPGNSQTIK*NWRTRAC
KCSHKPRRAR*T*K*AQADKSPVDKGFQSFT*E
TRRN*SSNKRPWAA*KKA*RP*RAVKSSAAVVI
SY*ESVGNL*PTKPRRTI*R*GN*NSSSS*TTGC
GRDFV*RAAFVQGKTSHSASEEEVRRSEL*VEG
GKPFTSRAEGKAA*PSSWTDHYWSLSYSDCYSG
DTTCGY*GNCHLQTRNAIFLDVGGTCSGRFQPGL
DRTYRLAFSA*SSYKITEGDGG*P*GYQRDDHQ
AEGNNAGFGTEASPVGRTHYRCPKFEKQDQQSR
G*NNHYGSN*KNSESVG*STRTPSEPEATVE*NV
KGFNTMAGS*GRS*AGLRTGQSQA*VMEGGSLY
SRCNPKENHRNQAVGQRPPPVADKCRCGK*LGP
ETSPGLFCR*YQKSPHDNREYQCLLEKHS*KGE*
ARGCFGRNS*ITATVPPGPGKVSCLAYRS*NNC
QCPTGCYP*GKAPRRLQGSKRADETMARPPR*N
*SSHRCLSQPG*KQPKNPEIPGRFR*CSPVTKTF
G*HELQVE*TSEKVSQH*VPFGSQF*PVEASAP
FSAGTSGVATAER**IKPAGTYWRRLSSSSEAE
RCT*GLQEGIEN*RTCNHEYS*DCTNISDRAAFG
RTRETLPGAQRAAS*GESPECHSASTKAG*GGQ
Y*VGKIEPALR*LAEKNR*DP*KTPGTSRGHG*
AGPQAAPS*GDQGILAARGRSPH*LSPRSPRESQ
GTSRRNCASEREREPRQ*PCSPAYHFGHSALTV
*PQHSGRPEHQMEASAGGRRGPSQAAA*SPQGL
WSSISALSFHVCPGSLGESHLAKQSALLYQPRDS
NNLLGPSQNDRALPVFS*PE*CQILSL*DCHET
PKTAEGPLLGSLEPVSCM*CLGPAQPQAK*PAH
GYPADY*LFDHYL*PPGARAQQFGQRPSLRGYVS
ELAAECL*YGTNREDPCPVF*NWHHFPV*STFG
RQVQIPFQASGKFNRIL*PAQAGPPSA*FYPNS
KTVG*SCILWGQ*H*AKCPELLPIC***ARDRSG
PLPRLDETGTPVHGVAARPAQSGCCRNCQASGQ
M*HLQRVSNHWIQVQESKAL*L*HLPKLLFFWS
SCKRP*NALSHGGILHSDYIRRRCSRLCQGTKKQ
ISNQKVFCEASPNGLPASADCLRGGQHGNSRYS
DQLLASRFCACLVPSAFTR*YSFTH*TLC*QAS
RNGKQQWILSK**HLS**EHR**TFVNPALLPKF
EPGLPPEPAS*SCPDLDFLRE*GKRGARENPSR
S*GRKQESASRI*PSKAAART*RPVPTAVPS*N
DAHLSPESPGC*AHC*GQATASTQRPPGSQDANP
GRPQ*TAGVTVTQAKAAAGATPGRGQSEWHNGV
LSFYLSTEVRQQSAYAAPSGWQSNFGLHG*GRS
SQSSPGHKHRVRGGDGATQQLLP*FKRKKYPWKA
NERGHNVGSLFHMADDLGRAMESLVSVMTDEEG
AE*MFYNS*FPHGFYNIHTTKRIRQ*EFTRNKS
IFL*RVVVLYCRFQ*FLSLLLFC*QWQVLHVYAI
VQKSYKKTTCKILIAK*LAISLYGTHFGLFKNL
*QL*RKIVN*SVLYKKKLFIKTPKNKTNTHTHT
YTHTHKTLRQRIVLHPFGVISI*NSWLFLFLHIK
DKTSSTTTPNDYYTLLI*ELSAEWGRLEFSFHI
SICL*VYKYYSYIDKEIRISID*LFPFFKCSCH
ILIERNYF*SVIQAYLLGLEWIFPGAGSQEETTP
H*NIVYSSRCFSF*TTFH*QRK*SKVLDFFKGN
M*MNTQDLLYQSE*SVGWLID*LIDTFSFLLLA
MPRFRFNDASVEINQKVF*PCEHQKVFFNSQAVA
GR**GWRAMDSQPIPVKE*ATL*VKDWMIVHNT
*SSL*LQLNYYALFSQSKGTGWFGFCCFFRFIV
PCGMSF*MPQDII*NK*TLGKGVKQ*PHHICDTD
RYQPRSP*TVFPSFAFLCE*FHTGL*VSKKEGK
LIQMLQKNPSWWIRQVKYINKQTKIAQKRGEKL
KRKS*GLVGKSFTLSCHFISF*FLNHSFNRYHRV
TYNFANLLPLTSSVISFWRVG*HQV*LAFGEWV
LSIINN*LINIKHGFSCYFYLTLVLGCS**LCT
PEFTASPLVHCVIFFFLYNSFFFLHNFQKKTQSS
KVTNYQIT*RFGFCLAFFSFM*RWTFSLPKDF*
NSDLKQGVTLHPTKKFK*VSFILKSEVNRVHK*
FCFNLFVFLLDTLALE*VCHNI*TKIESFIAAF*
A*LIWTLFRVVFFITTKY*TVNHNVTEA*TSHG
MFCHCFQVLSSYLSIIIYCVLTPTL*HLRIIFL
NFSFTAFSQHIRLHQIYALLLYYSTALLCISIKH
AVML
Frame 2
MRSGGTFDLRKTDCND*DDFANFFSSLFP*CT*YCD*FSCRDP*SYSFVCYLFFSFTFFFYQQS*M
SARKLRNLSYKKAVRRQKLLEQSIQSAQETEKS
LHLIQESLTFIDKQLAAYIADKVDAAQMPQEAQK
IQSDLTSHEISLEEMKKHNQGKEAAQRVLSQID
VAQKKLQDVSMKFRLFQKPANFEQRLQESKMIL
DEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSL
SEVKSEVEMVIKTGRQIVQKKQTENPKELDERV
TALKLHYNELGAKVTERKQQLEKCLKLSRKMRK
EMNVLTEWLAATDMELTKRSAVEGMPSNLDSEVA
WGKATQKEIEKQKVHLKSITEVGEALKTVLGKK
ETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQ
KHMETFDQNVDHITKWIIQADTLLDESEKKKPQQ
KEDVLKRLKAELNDIRPKVDSTRDQAANLMANR
GDHCRKLVEPQISELNHRFAAISHRIKTGKASI
PLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDF
NKDMNEDNEGTVKELLQRGDNLQQRITDERKRE
EIKIKQQLLQTKHNALKDLRSQRRKKALEISHQ
WYQYKRQADDLLKCLDDIEKKLASLPEPRDERKI
KEIDRELQKKKEELNAVRRQAEGLSEDGAAMAV
EPTQIQLSKRWREIESKFAQFRRLNFAQIHTVR
EETMMVMTEDMPLEISYVPSTYLTEITHVSQALL
EVEQLLNAPDLCAKDFEDLFKQEESLKNIKDSL
QQSSGRIDIIHSKKTAALQSATPVERVKLQEAL
SQLDFQWEKVNKMYKDRQGRFDRSVEKWRRFHYD
IKIFNQWLTEAEQFLRKTQIPENWEHAKYKWYL
KELQDGIGQRQTVVRTLNATGEEIIQQSSKTDA
SILQEKLGSLNLRWQEVCKQLSDRKKRLEEQKNI
LSEFQRDLNEFVLWLEEADNIASIPLEPGKEQQ
LKEKLEQVKLLVEELPLRQGILKQLNETGGPVL
VSAPISPEEQDKLENKLKQTNLQWIKVSRALPEK
QGEIEAQIKDLGQLEKKLEDLEEQLNHLLLWLS
PIRNQLEIYNQPNQEGPFDVKETEIAVQAKQPD
VEEILSKGQHLYKEKPATQPVKRKLEDLSSEWKA
VNRLLQELRAKQPDLAPGLTTIGASPTQTVTLV
TQPVVTKETAISKLEMPSSLMLEVPALADFNRA
WTELTDWLSLLDQVIKSQRVMVGDLEDINEMIIK
QKATMQDLEQRRPQLEELITAAQNLKNKTSNQE
ARTIITDRIERIQNQWDEVQEHLQNRRQQLNEM
LKDSTQWLEAKEEAEQVLGQARAKLESWKEGPYT
VDAIQKKITETKQLAKDLRQWQTNVDVANDLAL
KLLRDYSADDTRKVHMITENINASWRSIHKRVS
EREAALEETHRLLQQFPLDLEKFLAWLTEAETTA
NVLQDATRKERLLEDSKGVKELMKQWQDLQGEI
EAHTDVYHNLDENSQKILRSLEGSDDAVLLQRR
LDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHL
SLQELLVWLQLKDDELSRQAPIGGDFPAVQKQN
DVHRAFKRELKTKEPVIMSTLETVRIFLTEQPL
EGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVN
TEWEKLNLHSADWQRKIDETLERLRELQEATDE
LDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKV
KALRGEIAPLKENVSHVNDLARQLTTLGIQLSPY
NLSTLEDLNTRWKLLQVAVEDRVRQLHEAHRDF
GPASQHFLSTSVQGPWERAISPNKVPYYINHET
QTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKL
RRLQKALCLDLLSLSAACDALDQHNLKQNDQPM
DILQIINCLTTIYDRLEQEHNNLVNVPLCVDMC
LNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLE
DKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIP
RQLGEVASFGGSNIEPSVRSCFQFANNKPEIEA
ALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAK
CNICKECPIIGFRYRSLKHFNYDICQSCFFSGR
VAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKN
KFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTL
INFWPVDSAPASSPQLSHDDTHSRIEHYASRLA
EMENSNGSYLNDSISPNESIDDEHLLIQHYCQS
LNQDSPLSQPRSPAQILISLESEERGELERILAD
LEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEM
MPTSPQSPRDAELIAEAKLLRQHKGRLEARMQI
LEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVS
SPSTSLQRSDSSQPMLLRVVGSQTSDSMGEEDL
LSPPQDTSTGLEEVMEQLNNSFPSSRGRNTPGK
PMREDTM*EVFSTWQMIWAERWSP*YQS*QMKKE
QNKCFTTPDSRMVFIIFIQQRGLDSKSLQEINL
YFCEG*WYYTVDFSSF*VCYCFVNNGRFYTSMQ
LYKKVIRKLHVKS**LNNLPFLYMERILGCLKIY
NSYKERL*TKVCFIKKSCL*KPLKTKQTHTHTH
THTHTKL*GSALFCILLA*YPYEIHGFFFFCIL
KIRLPLPPHQMTTTHCSFENCQLSGAGLSFHFIY
LYVYKYINTIVI*IKRYEFL*TDFFHFLNVHVT
S**KEITSSQSSRLTCLV*NGFFPEPEARRKLH
HTKTLSTAPDVSHFKQLSTDNESKVKYWIFLKGT
CE*IHRTYYIRVSNRLVG*LID*LIHSASCC*Q
CHDLDLMMLQWKSIRRYSDLVNIRRYFLTPKQ*
QDDDRAGGLWIPSPSL*RSRPLFK*RIG*LFIIH
KVLCNYN*IIMPSSHSQKELGGLVFVAFLDLLS
HVG*VFKCHKT*FKINKLWEKV*NSSPITFVIL
TGINPEAHELCFHPLHFSASSSTQVCK*VRKKAN
*FKCYKKTLLGGLDRLNI*TNKQKLLKKEEKSS
RGKAKDW*EKALLFHAILFLFDF*IIHSIDTTV
*PIILQICYL*HQV*LAFGEWADIKCN*LLESGF
CPLLIIN*LTSNTASHAISTSLWFWGVPDNCAH
LSSQLHHLSIALFSFSFIILSFSFIIFKRKPKA
LR*QITKLHEDLVFVLHFFPLCDAGPFLYPRIFK
TQI*NKGLLYILLRSLSK*VSF*NQR*IECINN
FVLIFLFFF*TH*LWSESVIIFEQKLRALLLHF
KHN*FGHYFVLCSL*PPSIKL*IIM*LKHKHHMA
CFVIVFRY*VLT*VS*YIVF*HQHCNIYELFF*
TSVLLHFHNISDFTKYMPYYCIIVLLYCVSQ*S
TQLCY
Sequence NM_004014
GGGTTTTCTCAGGATTGCTATGCAACAGGATCAGTGCTGTAGTGCCCGGTTCAAGCTGAAAATGTTACACAGGAAGACATACCATGTAAAGGACCTCCAAGGTGAAATTGAAGCTCACACAGATGTTTATCACAACCTGGATGAAAACAGCCAAAAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTAC
AAAGACGTTTGGATAACATGAACTTCAAGTGGAGTGAACTTCGGAAAAAGTCTCTCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCG
TCTGCACCTTTCTCTGCAGGAACTTCTGGTGTGGCTACAGCTGAAAGATGATGAATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAG
AAGCAGAACGATGTACATAGGGCCTTCAAGAGGGAATTGAAAACTAAAGAACCTGTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGC
AGCCTTTGGAAGGACTAGAGAAACTCTACCAGGAGCCCAGAGAGCTGCCTCCTGAGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGA
GGAGGTCAATACTGAGTGGGAAAAATTGAACCTGCACTCCGCTGACTGGCAGAGAAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCC
ACGGATGAGCTGGACCTCAAGCTGCGCCAAGCTGAGGTGATCAAGGGATCCTGGCAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCG
AGAAAGTCAAGGCACTTCGAGGAGAAATTGCGCCTCTGAAAGAGAACGTGAGCCACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCT
CTCACCGTATAACCTCAGCACTCTGGAAGACCTGAACACCAGATGGAAGCTTCTGCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCAC
AGGGACTTTGGTCCAGCATCTCAGCACTTTCTTTCCACGTCTGTCCAGGGTCCCTGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACC
ACGAGACTCAAACAACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGC
CATGAAACTCCGAAGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGAC
CAGCCCATGGATATCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGG
ATATGTGTCTGAACTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGC
ACATTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATC
CAAATTCCAAGACAGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGA
TCGAAGCGGCCCTCTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCA
TCAGGCCAAATGTAACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTT
TCTGGTCGAGTTGCAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTAC
TAAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCC
CGTTACTCTGATCAACTTCTGGCCAGTAGATTCTGCGCCTGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGC
AGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATCTAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACT
GCCAAAGTTTGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAAT
CCTAGCAGATCTTGAGGAAGAAAACAGGAATCTGCAAGCAGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCT
CCTGAAATGATGCCCACCTCTCCCCAGAGTCCCCGGGATGCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGA
TGCAAATCCTGGAAGACCACAATAAACAGCTGGAGTCACAGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCAC
AACGGTGTCCTCTCCTTCTACCTCTCTACAGAGGTCCGACAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAG
GAAGATCTTCTCAGTCCTCCCCAGGACACAAGCACAGGGTTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCC
CTGGAAAGCCAATGAGAGAGGACACAATGTAGGAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGAT
GAAGAAGGAGCAGAATAAATGTTTTACAACTCCTGATTCCCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAA
ATAAATCTATATTTTTGTGAAGGGTAGTGGTATTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGT
CTATGCAATTGTACAAAAAAGTTATAAGAAAACTACATGTAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTT
AAAAATTTATAACAGTTATAAAGAAAGATTGTAAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACA
CACACACATACACACACACACACAAAACTTTGAGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTT
GCATATTAAAGATAAGACTTCCTCTACCACCACACCAAATGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCA
TTTCATATATCTATATGTCTATAAGTATATAAATACTATAGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTT
CATGTCACATCCTAATAGAAAGAAATTACTTCTAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGA
AACTACACCACACTAAAACATTGTCTACAGCTCCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTT
AAAGGGAACATGTGAATGAATACACAGGACTTATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGC
TGCTAGCAATGCCACGATTTAGATTTAATGATGCTTCAGTGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCA
AGCAGTAGCAGGACGATGATAGGGCTGGAGGGCTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTT
CATAATACATAAAGTTCTCTGTAATTACAACTAAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGAT
TTATTGTCCCATGTGGGATGAGTTTTTAAATGCCACAAGACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTG
TGATACTGACAGGTATCAACCCAGAAGCCCATGAACTGTGTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAA
GAAGGCAAATTGATTCAAATGTTACAAAAAAACCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAG
AAAAGCTCAAGAGGAAAAGCTAAGGACTGGTAGGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATA
CCACCGTGTGACCTATAATTTTGCAAATCTGTTACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGA
GAGTGGGTTTTGTCCATTATTAATAATTAATTAATTAACATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAAT
TGTGCACACCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAAC
CCAAAGCTCTAAGGTAACAAATTACCAAATTACATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAG
GATTTTTAAAACTCAGATTTAAAACAAGGGGTTACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGC
ATAAATAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAGACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGC
TGCATTTTAAGCATAATTAATTTGGACATTATTTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACA
TCACATGGCATGTTTTGTCATTGTTTTCAGGTACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTA
TTTTTTTAAACTTCAGTTTTACTGCATTTTCACAACATATCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTC
AATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
GFSQDCYATGSVL*CPVQAENVTQEDIPCKGPPR*N*SSHRCLSQPG*KQPKNPEIPGRFR*CSPVT
KTFG*HELQVE*TSEKVSQH*VPFGSQF*PVEA
SAPFSAGTSGVATAER**IKPAGTYWRRLSSSS
EAERCT*GLQEGIEN*RTCNHEYS*DCTNISDRA
AFGRTRETLPGAQRAAS*GESPECHSASTKAG*
GGQY*VGKIEPALR*LAEKNR*DP*KTPGTSRG
HG*AGPQAAPS*GDQGILAARGRSPH*LSPRSPR
ESQGTSRRNCASEREREPRQ*PCSPAYHFGHSA
LTV*PQHSGRPEHQMEASAGGRRGPSQAAA*SP
QGLWSSISALSFHVCPGSLGESHLAKQSALLYQP
RDSNNLLGPSQNDRALPVFS*PE*CQILSL*DC
HETPKTAEGPLLGSLEPVSCM*CLGPAQPQAK*
PAHGYPADY*LFDHYL*PPGARAQQFGQRPSLRG
YVSELAAECL*YGTNREDPCPVF*NWHHFPV*S
TFGRQVQIPFQASGKFNRIL*PAQAGPPSA*FY
PNSKTVG*SCILWGQ*H*AKCPELLPIC***ARD
RSGPLPRLDETGTPVHGVAARPAQSGCCRNCQA
SGQM*HLQRVSNHWIQVQESKAL*L*HLPKLLF
FWSSCKRP*NALSHGGILHSDYIRRRCSRLCQGT
KKQISNQKVFCEASPNGLPASADCLRGGQHGNS
RYSDQLLASRFCACLVPSAFTR*YSFTH*TLC*
QASRNGKQQWILSK**HLS**EHR**TFVNPALL
PKFEPGLPPEPAS*SCPDLDFLRE*GKRGAREN
PSRS*GRKQESASRI*PSKAAART*RPVPTAVP
S*NDAHLSPESPGC*AHC*GQATASTQRPPGSQD
ANPGRPQ*TAGVTVTQAKAAAGATPGRGQSEWH
NGVLSFYLSTEVRQQSAYAAPSGWQSNFGLHG*
GRSSQSSPGHKHRVRGGDGATQQLLP*FKRKKYP
WKANERGHNVGSLFHMADDLGRAMESLVSVMTD
EEGAE*MFYNS*FPHGFYNIHTTKRIRQ*EFTR
NKSIFL*RVVVLYCRFQ*FLSLLLFC*QWQVLHV
YAIVQKSYKKTTCKILIAK*LAISLYGTHFGLF
KNL*QL*RKIVN*SVLYKKKLFIKTPKNKTNTH
THTYTHTHKTLRQRIVLHPFGVISI*NSWLFLFL
HIKDKTSSTTTPNDYYTLLI*ELSAEWGRLEFS
FHISICL*VYKYYSYIDKEIRISID*LFPFFKC
SCHILIERNYF*SVIQAYLLGLEWIFPGAGSQEE
TTPH*NIVYSSRCFSF*TTFH*QRK*SKVLDFF
KGNM*MNTQDLLYQSE*SVGWLID*LIDTFSFL
LLAMPRFRFNDASVEINQKVF*PCEHQKVFFNSQ
AVAGR**GWRAMDSQPIPVKE*ATL*VKDWMIV
HNT*SSL*LQLNYYALFSQSKGTGWFGFCCFFR
FIVPCGMSF*MPQDII*NK*TLGKGVKQ*PHHIC
DTDRYQPRSP*TVFPSFAFLCE*FHTGL*VSKK
EGKLIQMLQKNPSWWIRQVKYINKQTKIAQKRG
EKLKRKS*GLVGKSFTLSCHFISF*FLNHSFNRY
HRVTYNFANLLPLTSSVISFWRVG*HQV*LAFG
EWVLSIINN*LINIKHGFSCYFYLTLVLGCS**
LCTPEFTASPLVHCVIFFFLYNSFFFLHNFQKKT
QSSKVTNYQIT*RFGFCLAFFSFM*RWTFSLPK
DF*NSDLKQGVTLHPTKKFK*VSFILKSEVNRV
HK*FCFNLFVFLLDTLALE*VCHNI*TKIESFIA
AF*A*LIWTLFRVVFFITTKY*TVNHNVTEA*T
SHGMFCHCFQVLSSYLSIIIYCVLTPTL*HLRI
IFLNFSFTAFSQHIRLHQIYALLLYYSTALLCIS
IKHAVML
Frame 1
GFLRIAMQQDQCCSARFKLKMLHRKTYHVKDLQGEIEAHTDVYHNLDENSQKILRSLEGSDDAVLL
QRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKR
LHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQ
KQNDVHRAFKRELKTKEPVIMSTLETVRIFLTE
QPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAE
EVNTEWEKLNLHSADWQRKIDETLERLRELQEA
TDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHL
EKVKALRGEIAPLKENVSHVNDLARQLTTLGIQL
SPYNLSTLEDLNTRWKLLQVAVEDRVRQLHEAH
RDFGPASQHFLSTSVQGPWERAISPNKVPYYIN
HETQTTCWDHPKMTELYQSLADLNNVRFSAYRTA
MKLRRLQKALCLDLLSLSAACDALDQHNLKQND
QPMDILQIINCLTTIYDRLEQEHNNLVNVPLCV
DMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKA
HLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSI
QIPRQLGEVASFGGSNIEPSVRSCFQFANNKPE
IEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKH
QAKCNICKECPIIGFRYRSLKHFNYDICQSCFF
SGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKV
LKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETP
VTLINFWPVDSAPASSPQLSHDDTHSRIEHYAS
RLAEMENSNGSYLNDSISPNESIDDEHLLIQHY
CQSLNQDSPLSQPRSPAQILISLESEERGELERI
LADLEEENRNLQAEYDRLKQQHEHKGLSPLPSP
PEMMPTSPQSPRDAELIAEAKLLRQHKGRLEAR
MQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGT
TVSSPSTSLQRSDSSQPMLLRVVGSQTSDSMGE
EDLLSPPQDTSTGLEEVMEQLNNSFPSSRGRNT
PGKPMREDTM*EVFSTWQMIWAERWSP*YQS*QM
KKEQNKCFTTPDSRMVFIIFIQQRGLDSKSLQE
INLYFCEG*WYYTVDFSSF*VCYCFVNNGRFYT
SMQLYKKVIRKLHVKS**LNNLPFLYMERILGCL
KIYNSYKERL*TKVCFIKKSCL*KPLKTKQTHT
HTHTHTHTKL*GSALFCILLA*YPYEIHGFFFF
CILKIRLPLPPHQMTTTHCSFENCQLSGAGLSFH
FIYLYVYKYINTIVI*IKRYEFL*TDFFHFLNV
HVTS**KEITSSQSSRLTCLV*NGFFPEPEARR
KLHHTKTLSTAPDVSHFKQLSTDNESKVKYWIFL
KGTCE*IHRTYYIRVSNRLVG*LID*LIHSASC
C*QCHDLDLMMLQWKSIRRYSDLVNIRRYFLTP
KQ*QDDDRAGGLWIPSPSL*RSRPLFK*RIG*LF
IIHKVLCNYN*IIMPSSHSQKELGGLVFVAFLD
LLSHVG*VFKCHKT*FKINKLWEKV*NSSPITF
VILTGINPEAHELCFHPLHFSASSSTQVCK*VRK
KAN*FKCYKKTLLGGLDRLNI*TNKQKLLKKEE
KSSRGKAKDW*EKALLFHAILFLFDF*IIHSID
TTV*PIILQICYL*HQV*LAFGEWADIKCN*LLE
SGFCPLLIIN*LTSNTASHAISTSLWFWGVPDN
CAHLSSQLHHLSIALFSFSFIILSFSFIIFKRK
PKALR*QITKLHEDLVFVLHFFPLCDAGPFLYPR
IFKTQI*NKGLLYILLRSLSK*VSF*NQR*IEC
INNFVLIFLFFF*TH*LWSESVIIFEQKLRALL
LHFKHN*FGHYFVLCSL*PPSIKL*IIM*LKHKH
HMACFVIVFRY*VLT*VS*YIVF*HQHCNIYEL
FF*TSVLLHFHNISDFTKYMPYYCIIVLLYCVS
Q*STQLCY
Frame 2
VFSGLLCNRISAVVPGSS*KCYTGRHTM*RTSKVKLKLTQMFITTWMKTAKKS*DPWKVPMMQSCY
KDVWIT*TSSGVNFGKSLSTLGPIWKPVLTSGS
VCTFLCRNFWCGYS*KMMN*AGRHLLEATFQQFR
SRTMYIGPSRGN*KLKNL*S*VLLRLYEYF*QS
SLWKD*RNSTRSPESCLLRREPRMSLGFYESRL
RRSILSGKN*TCTPLTGREK*MRPLKDSGNFKRP
RMSWTSSCAKLR*SRDPGSPWAISSLTLSKITS
RKSRHFEEKLRL*KRT*ATSMTLLASLPLWAFS
SHRITSALWKT*TPDGSFCRWPSRTESGSCMKPT
GTLVQHLSTFFPRLSRVPGREPSRQTKCPTIST
TRLKQLAGTIPK*QSSTSL*LT*IMSDSQLIGL
P*NSEDCRRPFAWIS*ACQLHVMPWTSTTSSKMT
SPWISCRLLIV*PLFMTAWSKSTTIWSTSLSAW
ICV*TGC*MFMIRDEQGGSVSCLLKLASFPCVK
HIWKTSTDTFSSKWQVQQDFVTSAGWASFCMILS
KFQDSWVKLHPLGAVTLSQVSGAASNLLIISQR
SKRPSS*TG*DWNPSPWCGCPSCTEWLLQKLPS
IRPNVTSAKSVQSLDSGTGV*STLIMTSAKAAFF
LVELQKAIKCTIPWWNIALRLHQEKMFETLPRY
*KTNFEPKGILRSIPEWATCQCRLS*RGTTWKL
PLL*STSGQ*ILRLPRPLSFHTMILIHALNIMLA
G*QKWKTAMDLI*MIASLLMRA*MMNIC*SSIT
AKV*TRTPP*ASLVVLPRS*FP*RVRKEGS*RE
S*QILRKKTGICKQNMTV*SSSTNIKACPHCRPL
LK*CPPLPRVPGMLSSLLRPSYCVNTKAAWKPG
CKSWKTTINSWSHSYTG*GSCWSNPRQRPK*MA
QRCPLLLPLYRGPTAVSLCCSEWLAVKLRTPWVR
KIFSVLPRTQAQG*RR*WSNSTTPSLVQEEEIP
LESQ*ERTQCRKSFPHGR*FGQSDGVLSISHDR
*RRSRINVLQLLIPAWFL*YSYNKED*TVRVYKK
*IYIFVKGSGIIL*ISVVSKSVIVLLTMAGFTR
LCNCTKKL*ENYM*NLDS*ITCHFFIWNAFWVV
*KFITVIKKDCKLKCAL*KKVVYKNP*KQNKHTH
THIHTHTQNFEAAHCFASFWRDIHMKFMAFSFF
AY*R*DFLYHHTK*LLHTAHLRTVS*VGQA*VF
ISYIYMSISI*IL*LYR*RDTNFYRLTFSIF*MF
MSHPNRKKLLLVSHPGLPAWSRMDFSRSRKPGG
NYTTLKHCLQLQMFLILNNFPLTTKVK*SIGFF
*REHVNEYTGLIISE*VIGWLVD*LID*YIQLPA
ASNATI*I**CFSGNQSEGILTL*TSEGIF*LP
SSSRTMIGLEGYGFPAHPCEGVGHSLSEGLDDC
S*YIKFSVITTKLLCPLLTVKRNWVVWFLLLF*I
YCPMWDEFLNATRHNLK*INFGKRCKTVAPSHL
*Y*QVSTQKPMNCVSILCISLRVVPHRFVSK*E
RRQIDSNVTKKPFLVD*TG*IYKQTNKNCSKKRR
KAQEEKLRTGRKKLYSFMPFYFFLIFKSFIQ*I
PPCDL*FCKSVTSDIKCN*LLESGLTSSVISFW
RVGFVHY**LIN*HQTRLLMLFLPHFGFGVFLII
VHT*VHSFTTCPLRYFLFPL*FFLFPS*FSKEN
PKL*GNKLPNYMKIWFLSCIFFLYVTLDLFFTQ
GFLKLRFKTRGYFTSY*EV*VSKFHSKIRGK*SA
*IILF*SFCFSFRHISSGVSLS*YLNKN*ELYC
CILSIINLDIISCCVLYNHQVLNCKS*CN*SIN
ITWHVLSLFSGTEFLLEYHNILCFNTNTVTFTNY
FFKLQFYCIFTTYQTSPNICLTIVL*YCFTVYL
NKARSYV
Sequence NM_004021
ACACTAGCAATGGCAAAGCTTTGTGCGGAGGCATTGCTGGCTGCTCTGAACTAAAAGCATCCGTGGGGACCGAAAGAGGTTTTTGCACACCTTATTAAGGAACTCCAGGATGGCATTGGGCAGCGGCAAACTGTTGTCAGAACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGATGCCAGTAT
TCTACAGGAAAAATTGGGAAGCCTGAATCTGCGGTGGCAGGAGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGGCTAGAAGAACAAAAGAATATCTTG
TCAGAATTTCAAAGAGATTTAAATGAATTTGTTTTATGGTTGGAGGAAGCAGATAACATTGCTAGTATCCCACTTGAACCTGGAAAAGAGCAGCAACTAA
AAGAAAAGCTTGAGCAAGTCAAGTTACTGGTGGAAGAGTTGCCCCTGCGCCAGGGAATTCTCAAACAATTAAATGAAACTGGAGGACCCGTGCTTGTAAG
TGCTCCCATAAGCCCAGAAGAGCAAGATAAACTTGAAAATAAGCTCAAGCAGACAAATCTCCAGTGGATAAAGGTTTCCAGAGCTTTACCTGAGAAACAA
GGAGAAATTGAAGCTCAAATAAAAGACCTTGGGCAGCTTGAAAAAAAGCTTGAAGACCTTGAAGAGCAGTTAAATCATCTGCTGCTGTGGTTATCTCCTA
TTAGGAATCAGTTGGAAATTTATAACCAACCAAACCAAGAAGGACCATTTGACGTTAAGGAAACTGAAATAGCAGTTCAAGCTAAACAACCGGATGTGGA
AGAGATTTTGTCTAAAGGGCAGCATTTGTACAAGGAAAAACCAGCCACTCAGCCAGTGAAGAGGAAGTTAGAAGATCTGAGCTCTGAGTGGAAGGCGGTA
AACCGTTTACTTCAAGAGCTGAGGGCAAAGCAGCCTGACCTAGCTCCTGGACTGACCACTATTGGAGCCTCTCCTACTCAGACTGTTACTCTGGTGACAC
AACCTGTGGTTACTAAGGAAACTGCCATCTCCAAACTAGAAATGCCATCTTCCTTGATGTTGGAGGTACCTGCTCTGGCAGATTTCAACCGGGCTTGGAC
AGAACTTACCGACTGGCTTTCTCTGCTTGATCAAGTTATAAAATCACAGAGGGTGATGGTGGGTGACCTTGAGGATATCAACGAGATGATCATCAAGCAG
AAGGCAACAATGCAGGATTTGGAACAGAGGCGTCCCCAGTTGGAAGAACTCATTACCGCTGCCCAAAATTTGAAAAACAAGACCAGCAATCAAGAGGCTA
GAACAATCATTACGGATCGAATTGAAAGAATTCAGAATCAGTGGGATGAAGTACAAGAACACCTTCAGAACCGGAGGCAACAGTTGAATGAAATGTTAAA
GGATTCAACACAATGGCTGGAAGCTAAGGAAGAAGCTGAGCAGGTCTTAGGACAGGCCAGAGCCAAGCTTGAGTCATGGAAGGAGGGTCCCTATACAGTA
GATGCAATCCAAAAGAAAATCACAGAAACCAAGCAGTTGGCCAAAGACCTCCGCCAGTGGCAGACAAATGTAGATGTGGCAAATGACTTGGCCCTGAAAC
TTCTCCGGGATTATTCTGCAGATGATACCAGAAAAGTCCACATGATAACAGAGAATATCAATGCCTCTTGGAGAAGCATTCATAAAAGGGTGAGTGAGCG
AGAGGCTGCTTTGGAAGAAACTCATAGATTACTGCAACAGTTCCCCCTGGACCTGGAAAAGTTTCTTGCCTGGCTTACAGAAGCTGAAACAACTGCCAAT
GTCCTACAGGATGCTACCCGTAAGGAAAGGCTCCTAGAAGACTCCAAGGGAGTAAAAGAGCTGATGAAACAATGGCAAGACCTCCAAGGTGAAATTGAAG
CTCACACAGATGTTTATCACAACCTGGATGAAAACAGCCAAAAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTACAAAGACGTTTGGA
TAACATGAACTTCAAGTGGAGTGAACTTCGGAAAAAGTCTCTCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCGTCTGCACCTTTCT
CTGCAGGAACTTCTGGTGTGGCTACAGCTGAAAGATGATGAATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAGAAGCAGAACGATG
TACATAGGGCCTTCAAGAGGGAATTGAAAACTAAAGAACCTGTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGCAGCCTTTGGAAGG
ACTAGAGAAACTCTACCAGGAGCCCAGAGAGCTGCCTCCTGAGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGAGGAGGTCAATACT
GAGTGGGAAAAATTGAACCTGCACTCCGCTGACTGGCAGAGAAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCCACGGATGAGCTGG
ACCTCAAGCTGCGCCAAGCTGAGGTGATCAAGGGATCCTGGCAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCGAGAAAGTCAAGGC
ACTTCGAGGAGAAATTGCGCCTCTGAAAGAGAACGTGAGCCACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCTCTCACCGTATAAC
CTCAGCACTCTGGAAGACCTGAACACCAGATGGAAGCTTCTGCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCACAGGGACTTTGGTC
CAGCATCTCAGCACTTTCTTTCCACGTCTGTCCAGGGTCCCTGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACCACGAGACTCAAAC
AACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGCCATGAAACTCCGA
AGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGACCAGCCCATGGATA
TCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGGATATGTGTCTGAA
CTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGCACATTTGGAAGAC
AAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATCCAAATTCCAAGAC
AGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGATCGAAGCGGCCCT
CTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCATCAGGCCAAATGT
AACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGTCGAGTTG
CAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTAAAAAACAAATT
TCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCCCGTTACTCTGATC
AACTTCTGGCCAGTAGATTCTGCGCCTGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCAGAAA
TGGAAAACAGCAATGGATCTTATCTAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTTTGAA
CCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTT
GAGGAAGAAAACAGGAATCTGCAAGCAGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATGATGC
CCACCTCTCCCCAGAGTCCCCGGGATGCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCCTGGA
AGACCACAATAAACAGCTGGAGTCACAGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTCCTCT
CCTTCTACCTCTCTACAGAGGTCCGACAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTTCTCA
GTCCTCCCCAGGACACAAGCACAGGGTTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGACACAATGTAGGAAGTCTTTTCCA
CATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGAGCAGAATAAATGTTTTACAACTCCTGATTCCCGCAT
GGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTATATTTTTGTGAAGGGTAGTGGTATTATACTGTAGAT
TTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTACAAAAAAGTTATAAGAAAACTACATGTAAAAT
CTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAACAGTTATAAAGAAAGATTGTAAACTAAAGTGTG
CTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACATACACACACACACACAAAACTTTGAGGCAGCGCATTG
TTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAAAGATAAGACTTCCTCTACCACCACACCAAATGACTA
CTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTATATGTCTATAAGTATATAAATACTATAGTTAT
ATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCTAATAGAAAGAAATTACTTCTAGTCAGTCATCC
AGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACCACACTAAAACATTGTCTACAGCTCCAGATGTTTCTC
ATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAACATGTGAATGAATACACAGGACTTATTATATCAGAGT
GAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCCACGATTTAGATTTAATGATGCTTCAGTGGAAA
TCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGACGATGATAGGGCTGGAGGGCTATGGATTCCCA
GCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACATAAAGTTCTCTGTAATTACAACTAAATTATTATGCC
CTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCCCATGTGGGATGAGTTTTTAAATGCCACAAGACATAA
TTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGGTATCAACCCAGAAGCCCATGAACTGTGTTTCC
ATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGATTCAAATGTTACAAAAAAACCCTTCTTGGTGG
ATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCAAGAGGAAAAGCTAAGGACTGGTAGGAAAAAGCTTTA
CTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACCGTGTGACCTATAATTTTGCAAATCTGTTACCTCTGACATC
AAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGTCCATTATTAATAATTAATTAATTAACATCAAA
CACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTA
TTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTCTAAGGTAACAAATTACCAAATTACATGAAGATTTGG
TTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAAAACTCAGATTTAAAACAAGGGGTTACTTTACATCCT
ACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTGTTTTAATCTTTTTGTTTTTCTTTTAGACACAT
TAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCATAATTAATTTGGACATTATTTCGTGTTGTGTT
CTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGCATGTTTTGTCATTGTTTTCAGGTACTGAGTTCTTAC
TTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTTTTAAACTTCAGTTTTACTGCATTTTCACAACATATCAGAC
TTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCAGTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
TLAMAKLCAEALLAALN*KHPWGPKEVFAHLIKELQDGIGQRQTVVRTLNATGEEIIQQSSKTDASI
LQEKLGSLNLRWQEVCKQLSDRKKRLEEQKNIL
SEFQRDLNEFVLWLEEADNIASIPLEPGKEQQL
KEKLEQVKLLVEELPLRQGILKQLNETGGPVLVS
APISPEEQDKLENKLKQTNLQWIKVSRALPEKQ
GEIEAQIKDLGQLEKKLEDLEEQLNHLLLWLSP
IRNQLEIYNQPNQEGPFDVKETEIAVQAKQPDVE
EILSKGQHLYKEKPATQPVKRKLEDLSSEWKAV
NRLLQELRAKQPDLAPGLTTIGASPTQTVTLVT
QPVVTKETAISKLEMPSSLMLEVPALADFNRAWT
ELTDWLSLLDQVIKSQRVMVGDLEDINEMIIKQ
KATMQDLEQRRPQLEELITAAQNLKNKTSNQEA
RTIITDRIERIQNQWDEVQEHLQNRRQQLNEMLK
DSTQWLEAKEEAEQVLGQARAKLESWKEGPYTV
DAIQKKITETKQLAKDLRQWQTNVDVANDLALK
LLRDYSADDTRKVHMITENINASWRSIHKRVSER
EAALEETHRLLQQFPLDLEKFLAWLTEAETTAN
VLQDATRKERLLEDSKGVKELMKQWQDLQGEIE
AHTDVYHNLDENSQKILRSLEGSDDAVLLQRRLD
NMNFKWSELRKKSLNIRSHLEASSDQWKRLHLS
LQELLVWLQLKDDELSRQAPIGGDFPAVQKQND
VHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEG
LEKLYQEPRELPPEERAQNVTRLLRKQAEEVNT
EWEKLNLHSADWQRKIDETLERLRELQEATDEL
DLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKA
LRGEIAPLKENVSHVNDLARQLTTLGIQLSPYN
LSTLEDLNTRWKLLQVAVEDRVRQLHEAHRDFG
PASQHFLSTSVQGPWERAISPNKVPYYINHETQT
TCWDHPKMTELYQSLADLNNVRFSAYRTAMKLR
RLQKALCLDLLSLSAACDALDQHNLKQNDQPMD
ILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLN
WLLNVYDTGRTGRIRVLSFKTGIISLCKAHLED
KYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPR
QLGEVASFGGSNIEPSVRSCFQFANNKPEIEAAL
FLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKC
NICKECPIIGFRYRSLKHFNYDICQSCFFSGRV
AKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKF
RTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLI
NFWPVDSAPASSPQLSHDDTHSRIEHYASRLAE
MENSNGSYLNDSISPNESIDDEHLLIQHYCQSLN
QDSPLSQPRSPAQILISLESEERGELERILADL
EEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMM
PTSPQSPRDAELIAEAKLLRQHKGRLEARMQILE
DHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSS
PSTSLQRSDSSQPMLLRVVGSQTSDSMGEEDLL
SPPQDTSTGLEEVMEQLNNSFPSSRGHNVGSLFH
MADDLGRAMESLVSVMTDEEGAE*MFYNS*FPH
GFYNIHTTKRIRQ*EFTRNKSIFL*RVVVLYCR
FQ*FLSLLLFC*QWQVLHVYAIVQKSYKKTTCKI
LIAK*LAISLYGTHFGLFKNL*QL*RKIVN*SV
LYKKKLFIKTPKNKTNTHTHTYTHTHKTLRQRI
VLHPFGVISI*NSWLFLFLHIKDKTSSTTTPNDY
YTLLI*ELSAEWGRLEFSFHISICL*VYKYYSY
IDKEIRISID*LFPFFKCSCHILIERNYF*SVI
QAYLLGLEWIFPGAGSQEETTPH*NIVYSSRCFS
F*TTFH*QRK*SKVLDFFKGNM*MNTQDLLYQS
E*SVGWLID*LIDTFSFLLLAMPRFRFNDASVE
INQKVF*PCEHQKVFFNSQAVAGR**GWRAMDSQ
PIPVKE*ATL*VKDWMIVHNT*SSL*LQLNYYA
LFSQSKGTGWFGFCCFFRFIVPCGMSF*MPQDI
I*NK*TLGKGVKQ*PHHICDTDRYQPRSP*TVFP
SFAFLCE*FHTGL*VSKKEGKLIQMLQKNPSWW
IRQVKYINKQTKIAQKRGEKLKRKS*GLVGKSF
TLSCHFISF*FLNHSFNRYHRVTYNFANLLPLTS
SVISFWRVG*HQV*LAFGEWVLSIINN*LINIK
HGFSCYFYLTLVLGCS**LCTPEFTASPLVHCV
IFFFLYNSFFFLHNFQKKTQSSKVTNYQIT*RFG
FCLAFFSFM*RWTFSLPKDF*NSDLKQGVTLHP
TKKFK*VSFILKSEVNRVHK*FCFNLFVFLLDT
LALE*VCHNI*TKIESFIAAF*A*LIWTLFRVVF
FITTKY*TVNHNVTEA*TSHGMFCHCFQVLSSY
LSIIIYCVLTPTL*HLRIIFLNFSFTAFSQHIR
LHQIYALLLYYSTALLCISIKHAVML
Frame 1
H*QWQSFVRRHCWLL*TKSIRGDRKRFLHTLLRNSRMALGSGKLLSEH*MQLGKK*FSNPQKQMPV
FYRKNWEA*ICGGRRSANSCQTEKRG*KNKRISC
QNFKEI*MNLFYGWRKQITLLVSHLNLEKSSN*
KKSLSKSSYWWKSCPCAREFSNN*MKLEDPCL*
VLP*AQKSKINLKISSSRQISSG*RFPELYLRNK
EKLKLK*KTLGSLKKSLKTLKSS*IICCCGYLL
LGISWKFITNQTKKDHLTLRKLK*QFKLNNRMW
KRFCLKGSICTRKNQPLSQ*RGS*KI*ALSGRR*
TVYFKS*GQSSLT*LLD*PLLEPLLLRLLLW*H
NLWLLRKLPSPN*KCHLP*CWRYLLWQISTGLG
QNLPTGFLCLIKL*NHRG*WWVTLRISTR*SSSR
RQQCRIWNRGVPSWKNSLPLPKI*KTRPAIKRL
EQSLRIELKEFRISGMKYKNTFRTGGNS*MKC*
RIQHNGWKLRKKLSRS*DRPEPSLSHGRRVPIQ*
MQSKRKSQKPSSWPKTSASGRQM*MWQMTWP*N
FSGIILQMIPEKST**QRISMPLGEAFIKG*VS
ERLLWKKLIDYCNSSPWTWKSFLPGLQKLKQLPM
SYRMLPVRKGS*KTPRE*KS**NNGKTSKVKLK
LTQMFITTWMKTAKKS*DPWKVPMMQSCYKDVW
IT*TSSGVNFGKSLSTLGPIWKPVLTSGSVCTFL
CRNFWCGYS*KMMN*AGRHLLEATFQQFRSRTM
YIGPSRGN*KLKNL*S*VLLRLYEYF*QSSLWK
D*RNSTRSPESCLLRREPRMSLGFYESRLRRSIL
SGKN*TCTPLTGREK*MRPLKDSGNFKRPRMSW
TSSCAKLR*SRDPGSPWAISSLTLSKITSRKSR
HFEEKLRL*KRT*ATSMTLLASLPLWAFSSHRIT
SALWKT*TPDGSFCRWPSRTESGSCMKPTGTLV
QHLSTFFPRLSRVPGREPSRQTKCPTISTTRLK
QLAGTIPK*QSSTSL*LT*IMSDSQLIGLP*NSE
DCRRPFAWIS*ACQLHVMPWTSTTSSKMTSPWI
SCRLLIV*PLFMTAWSKSTTIWSTSLSAWICV*
TGC*MFMIRDEQGGSVSCLLKLASFPCVKHIWKT
STDTFSSKWQVQQDFVTSAGWASFCMILSKFQD
SWVKLHPLGAVTLSQVSGAASNLLIISQRSKRP
SS*TG*DWNPSPWCGCPSCTEWLLQKLPSIRPNV
TSAKSVQSLDSGTGV*STLIMTSAKAAFFLVEL
QKAIKCTIPWWNIALRLHQEKMFETLPRY*KTN
FEPKGILRSIPEWATCQCRLS*RGTTWKLPLL*S
TSGQ*ILRLPRPLSFHTMILIHALNIMLAG*QK
WKTAMDLI*MIASLLMRA*MMNIC*SSITAKV*
TRTPP*ASLVVLPRS*FP*RVRKEGS*RES*QIL
RKKTGICKQNMTV*SSSTNIKACPHCRPLLK*C
PPLPRVPGMLSSLLRPSYCVNTKAAWKPGCKSW
KTTINSWSHSYTG*GSCWSNPRQRPK*MAQRCPL
LLPLYRGPTAVSLCCSEWLAVKLRTPWVRKIFS
VLPRTQAQG*RR*WSNSTTPSLVQEDTM*EVFS
TWQMIWAERWSP*YQS*QMKKEQNKCFTTPDSRM
VFIIFIQQRGLDSKSLQEINLYFCEG*WYYTVD
FSSF*VCYCFVNNGRFYTSMQLYKKVIRKLHVK
S**LNNLPFLYMERILGCLKIYNSYKERL*TKVC
FIKKSCL*KPLKTKQTHTHTHTHTHTKL*GSAL
FCILLA*YPYEIHGFFFFCILKIRLPLPPHQMT
TTHCSFENCQLSGAGLSFHFIYLYVYKYINTIVI
*IKRYEFL*TDFFHFLNVHVTS**KEITSSQSS
RLTCLV*NGFFPEPEARRKLHHTKTLSTAPDVS
HFKQLSTDNESKVKYWIFLKGTCE*IHRTYYIRV
SNRLVG*LID*LIHSASCC*QCHDLDLMMLQWK
SIRRYSDLVNIRRYFLTPKQ*QDDDRAGGLWIP
SPSL*RSRPLFK*RIG*LFIIHKVLCNYN*IIMP
SSHSQKELGGLVFVAFLDLLSHVG*VFKCHKT*
FKINKLWEKV*NSSPITFVILTGINPEAHELCF
HPLHFSASSSTQVCK*VRKKAN*FKCYKKTLLGG
LDRLNI*TNKQKLLKKEEKSSRGKAKDW*EKAL
LFHAILFLFDF*IIHSIDTTV*PIILQICYL*H
QV*LAFGEWADIKCN*LLESGFCPLLIIN*LTSN
TASHAISTSLWFWGVPDNCAHLSSQLHHLSIAL
FSFSFIILSFSFIIFKRKPKALR*QITKLHEDL
VFVLHFFPLCDAGPFLYPRIFKTQI*NKGLLYIL
LRSLSK*VSF*NQR*IECINNFVLIFLFFF*TH
*LWSESVIIFEQKLRALLLHFKHN*FGHYFVLC
SL*PPSIKL*IIM*LKHKHHMACFVIVFRY*VLT
*VS*YIVF*HQHCNIYELFF*TSVLLHFHNISD
FTKYMPYYCIIVLLYCVSQ*STQLCY
Frame 2
TSNGKALCGGIAGCSELKASVGTERGFCTPY*GTPGWHWAAANCCQNIECNWGRNNSAILKNRCQY
STGKIGKPESAVAGGLQTAVRQKKEARRTKEYL
VRISKRFK*ICFMVGGSR*HC*YPT*TWKRAATK
RKA*ASQVTGGRVAPAPGNSQTIK*NWRTRACK
CSHKPRRAR*T*K*AQADKSPVDKGFQSFT*ET
RRN*SSNKRPWAA*KKA*RP*RAVKSSAAVVISY
*ESVGNL*PTKPRRTI*R*GN*NSSSS*TTGCG
RDFV*RAAFVQGKTSHSASEEEVRRSEL*VEGG
KPFTSRAEGKAA*PSSWTDHYWSLSYSDCYSGDT
TCGY*GNCHLQTRNAIFLDVGGTCSGRFQPGLD
RTYRLAFSA*SSYKITEGDGG*P*GYQRDDHQA
EGNNAGFGTEASPVGRTHYRCPKFEKQDQQSRG*
NNHYGSN*KNSESVG*STRTPSEPEATVE*NVK
GFNTMAGS*GRS*AGLRTGQSQA*VMEGGSLYS
RCNPKENHRNQAVGQRPPPVADKCRCGK*LGPET
SPGLFCR*YQKSPHDNREYQCLLEKHS*KGE*A
RGCFGRNS*ITATVPPGPGKVSCLAYRS*NNCQ
CPTGCYP*GKAPRRLQGSKRADETMARPPR*N*S
SHRCLSQPG*KQPKNPEIPGRFR*CSPVTKTFG
*HELQVE*TSEKVSQH*VPFGSQF*PVEASAPF
SAGTSGVATAER**IKPAGTYWRRLSSSSEAERC
T*GLQEGIEN*RTCNHEYS*DCTNISDRAAFGR
TRETLPGAQRAAS*GESPECHSASTKAG*GGQY
*VGKIEPALR*LAEKNR*DP*KTPGTSRGHG*AG
PQAAPS*GDQGILAARGRSPH*LSPRSPRESQG
TSRRNCASEREREPRQ*PCSPAYHFGHSALTV*
PQHSGRPEHQMEASAGGRRGPSQAAA*SPQGLWS
SISALSFHVCPGSLGESHLAKQSALLYQPRDSN
NLLGPSQNDRALPVFS*PE*CQILSL*DCHETP
KTAEGPLLGSLEPVSCM*CLGPAQPQAK*PAHGY
PADY*LFDHYL*PPGARAQQFGQRPSLRGYVSE
LAAECL*YGTNREDPCPVF*NWHHFPV*STFGR
QVQIPFQASGKFNRIL*PAQAGPPSA*FYPNSKT
VG*SCILWGQ*H*AKCPELLPIC***ARDRSGP
LPRLDETGTPVHGVAARPAQSGCCRNCQASGQM
*HLQRVSNHWIQVQESKAL*L*HLPKLLFFWSSC
KRP*NALSHGGILHSDYIRRRCSRLCQGTKKQI
SNQKVFCEASPNGLPASADCLRGGQHGNSRYSD
QLLASRFCACLVPSAFTR*YSFTH*TLC*QASRN
GKQQWILSK**HLS**EHR**TFVNPALLPKFE
PGLPPEPAS*SCPDLDFLRE*GKRGARENPSRS
*GRKQESASRI*PSKAAART*RPVPTAVPS*NDA
HLSPESPGC*AHC*GQATASTQRPPGSQDANPG
RPQ*TAGVTVTQAKAAAGATPGRGQSEWHNGVL
SFYLSTEVRQQSAYAAPSGWQSNFGLHG*GRSSQ
SSPGHKHRVRGGDGATQQLLP*FKRTQCRKSFP
HGR*FGQSDGVLSISHDR*RRSRINVLQLLIPA
WFL*YSYNKED*TVRVYKK*IYIFVKGSGIIL*I
SVVSKSVIVLLTMAGFTRLCNCTKKL*ENYM*N
LDS*ITCHFFIWNAFWVV*KFITVIKKDCKLKC
AL*KKVVYKNP*KQNKHTHTHIHTHTQNFEAAHC
FASFWRDIHMKFMAFSFFAY*R*DFLYHHTK*L
LHTAHLRTVS*VGQA*VFISYIYMSISI*IL*L
YR*RDTNFYRLTFSIF*MFMSHPNRKKLLLVSHP
GLPAWSRMDFSRSRKPGGNYTTLKHCLQLQMFL
ILNNFPLTTKVK*SIGFF*REHVNEYTGLIISE
*VIGWLVD*LID*YIQLPAASNATI*I**CFSGN
QSEGILTL*TSEGIF*LPSSSRTMIGLEGYGFP
AHPCEGVGHSLSEGLDDCS*YIKFSVITTKLLC
PLLTVKRNWVVWFLLLF*IYCPMWDEFLNATRHN
LK*INFGKRCKTVAPSHL*Y*QVSTQKPMNCVS
ILCISLRVVPHRFVSK*ERRQIDSNVTKKPFLV
D*TG*IYKQTNKNCSKKRRKAQEEKLRTGRKKLY
SFMPFYFFLIFKSFIQ*IPPCDL*FCKSVTSDI
KCN*LLESGLTSSVISFWRVGFVHY**LIN*HQ
TRLLMLFLPHFGFGVFLIIVHT*VHSFTTCPLRY
FLFPL*FFLFPS*FSKENPKL*GNKLPNYMKIW
FLSCIFFLYVTLDLFFTQGFLKLRFKTRGYFTS
Y*EV*VSKFHSKIRGK*SA*IILF*SFCFSFRHI
SSGVSLS*YLNKN*ELYCCILSIINLDIISCCV
LYNHQVLNCKS*CN*SINITWHVLSLFSGTEFL
LEYHNILCFNTNTVTFTNYFFKLQFYCIFTTYQT
SPNICLTIVL*YCFTVYLNKARSYV
Sequence NM_004013
ACACTAGCAATGGCAAAGCTTTGTGCGGAGGCATTGCTGGCTGCTCTGAACTAAAAGCATCCGTGGGGACCGAAAGAGGTTTTTGCACACCTTATTAAGGAACTCCAGGATGGCATTGGGCAGCGGCAAACTGTTGTCAGAACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGATGCCAGTAT
TCTACAGGAAAAATTGGGAAGCCTGAATCTGCGGTGGCAGGAGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGGCTAGAAGAACAAAAGAATATCTTG
TCAGAATTTCAAAGAGATTTAAATGAATTTGTTTTATGGTTGGAGGAAGCAGATAACATTGCTAGTATCCCACTTGAACCTGGAAAAGAGCAGCAACTAA
AAGAAAAGCTTGAGCAAGTCAAGTTACTGGTGGAAGAGTTGCCCCTGCGCCAGGGAATTCTCAAACAATTAAATGAAACTGGAGGACCCGTGCTTGTAAG
TGCTCCCATAAGCCCAGAAGAGCAAGATAAACTTGAAAATAAGCTCAAGCAGACAAATCTCCAGTGGATAAAGGTTTCCAGAGCTTTACCTGAGAAACAA
GGAGAAATTGAAGCTCAAATAAAAGACCTTGGGCAGCTTGAAAAAAAGCTTGAAGACCTTGAAGAGCAGTTAAATCATCTGCTGCTGTGGTTATCTCCTA
TTAGGAATCAGTTGGAAATTTATAACCAACCAAACCAAGAAGGACCATTTGACGTTAAGGAAACTGAAATAGCAGTTCAAGCTAAACAACCGGATGTGGA
AGAGATTTTGTCTAAAGGGCAGCATTTGTACAAGGAAAAACCAGCCACTCAGCCAGTGAAGAGGAAGTTAGAAGATCTGAGCTCTGAGTGGAAGGCGGTA
AACCGTTTACTTCAAGAGCTGAGGGCAAAGCAGCCTGACCTAGCTCCTGGACTGACCACTATTGGAGCCTCTCCTACTCAGACTGTTACTCTGGTGACAC
AACCTGTGGTTACTAAGGAAACTGCCATCTCCAAACTAGAAATGCCATCTTCCTTGATGTTGGAGGTACCTGCTCTGGCAGATTTCAACCGGGCTTGGAC
AGAACTTACCGACTGGCTTTCTCTGCTTGATCAAGTTATAAAATCACAGAGGGTGATGGTGGGTGACCTTGAGGATATCAACGAGATGATCATCAAGCAG
AAGGCAACAATGCAGGATTTGGAACAGAGGCGTCCCCAGTTGGAAGAACTCATTACCGCTGCCCAAAATTTGAAAAACAAGACCAGCAATCAAGAGGCTA
GAACAATCATTACGGATCGAATTGAAAGAATTCAGAATCAGTGGGATGAAGTACAAGAACACCTTCAGAACCGGAGGCAACAGTTGAATGAAATGTTAAA
GGATTCAACACAATGGCTGGAAGCTAAGGAAGAAGCTGAGCAGGTCTTAGGACAGGCCAGAGCCAAGCTTGAGTCATGGAAGGAGGGTCCCTATACAGTA
GATGCAATCCAAAAGAAAATCACAGAAACCAAGCAGTTGGCCAAAGACCTCCGCCAGTGGCAGACAAATGTAGATGTGGCAAATGACTTGGCCCTGAAAC
TTCTCCGGGATTATTCTGCAGATGATACCAGAAAAGTCCACATGATAACAGAGAATATCAATGCCTCTTGGAGAAGCATTCATAAAAGGGTGAGTGAGCG
AGAGGCTGCTTTGGAAGAAACTCATAGATTACTGCAACAGTTCCCCCTGGACCTGGAAAAGTTTCTTGCCTGGCTTACAGAAGCTGAAACAACTGCCAAT
GTCCTACAGGATGCTACCCGTAAGGAAAGGCTCCTAGAAGACTCCAAGGGAGTAAAAGAGCTGATGAAACAATGGCAAGACCTCCAAGGTGAAATTGAAG
CTCACACAGATGTTTATCACAACCTGGATGAAAACAGCCAAAAAATCCTGAGATCCCTGGAAGGTTCCGATGATGCAGTCCTGTTACAAAGACGTTTGGA
TAACATGAACTTCAAGTGGAGTGAACTTCGGAAAAAGTCTCTCAACATTAGGTCCCATTTGGAAGCCAGTTCTGACCAGTGGAAGCGTCTGCACCTTTCT
CTGCAGGAACTTCTGGTGTGGCTACAGCTGAAAGATGATGAATTAAGCCGGCAGGCACCTATTGGAGGCGACTTTCCAGCAGTTCAGAAGCAGAACGATG
TACATAGGGCCTTCAAGAGGGAATTGAAAACTAAAGAACCTGTAATCATGAGTACTCTTGAGACTGTACGAATATTTCTGACAGAGCAGCCTTTGGAAGG
ACTAGAGAAACTCTACCAGGAGCCCAGAGAGCTGCCTCCTGAGGAGAGAGCCCAGAATGTCACTCGGCTTCTACGAAAGCAGGCTGAGGAGGTCAATACT
GAGTGGGAAAAATTGAACCTGCACTCCGCTGACTGGCAGAGAAAAATAGATGAGACCCTTGAAAGACTCCGGGAACTTCAAGAGGCCACGGATGAGCTGG
ACCTCAAGCTGCGCCAAGCTGAGGTGATCAAGGGATCCTGGCAGCCCGTGGGCGATCTCCTCATTGACTCTCTCCAAGATCACCTCGAGAAAGTCAAGGC
ACTTCGAGGAGAAATTGCGCCTCTGAAAGAGAACGTGAGCCACGTCAATGACCTTGCTCGCCAGCTTACCACTTTGGGCATTCAGCTCTCACCGTATAAC
CTCAGCACTCTGGAAGACCTGAACACCAGATGGAAGCTTCTGCAGGTGGCCGTCGAGGACCGAGTCAGGCAGCTGCATGAAGCCCACAGGGACTTTGGTC
CAGCATCTCAGCACTTTCTTTCCACGTCTGTCCAGGGTCCCTGGGAGAGAGCCATCTCGCCAAACAAAGTGCCCTACTATATCAACCACGAGACTCAAAC
AACTTGCTGGGACCATCCCAAAATGACAGAGCTCTACCAGTCTTTAGCTGACCTGAATAATGTCAGATTCTCAGCTTATAGGACTGCCATGAAACTCCGA
AGACTGCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGATGCCTTGGACCAGCACAACCTCAAGCAAAATGACCAGCCCATGGATA
TCCTGCAGATTATTAATTGTTTGACCACTATTTATGACCGCCTGGAGCAAGAGCACAACAATTTGGTCAACGTCCCTCTCTGCGTGGATATGTGTCTGAA
CTGGCTGCTGAATGTTTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCCCTGTGTAAAGCACATTTGGAAGAC
AAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACAGGATTTTGTGACCAGCGCAGGCTGGGCCTCCTTCTGCATGATTCTATCCAAATTCCAAGAC
AGTTGGGTGAAGTTGCATCCTTTGGGGGCAGTAACATTGAGCCAAGTGTCCGGAGCTGCTTCCAATTTGCTAATAATAAGCCAGAGATCGAAGCGGCCCT
CTTCCTAGACTGGATGAGACTGGAACCCCAGTCCATGGTGTGGCTGCCCGTCCTGCACAGAGTGGCTGCTGCAGAAACTGCCAAGCATCAGGCCAAATGT
AACATCTGCAAAGAGTGTCCAATCATTGGATTCAGGTACAGGAGTCTAAAGCACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGTCGAGTTG
CAAAAGGCCATAAAATGCACTATCCCATGGTGGAATATTGCACTCCGACTACATCAGGAGAAGATGTTCGAGACTTTGCCAAGGTACTAAAAAACAAATT
TCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCTGCCAGTGCAGACTGTCTTAGAGGGGGACAACATGGAAACTCCCGTTACTCTGATC
AACTTCTGGCCAGTAGATTCTGCGCCTGCCTCGTCCCCTCAGCTTTCACACGATGATACTCATTCACGCATTGAACATTATGCTAGCAGGCTAGCAGAAA
TGGAAAACAGCAATGGATCTTATCTAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAGTTTGAA
CCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAGGAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTT
GAGGAAGAAAACAGGAATCTGCAAGCAGAATATGACCGTCTAAAGCAGCAGCACGAACATAAAGGCCTGTCCCCACTGCCGTCCCCTCCTGAAATGATGC
CCACCTCTCCCCAGAGTCCCCGGGATGCTGAGCTCATTGCTGAGGCCAAGCTACTGCGTCAACACAAAGGCCGCCTGGAAGCCAGGATGCAAATCCTGGA
AGACCACAATAAACAGCTGGAGTCACAGTTACACAGGCTAAGGCAGCTGCTGGAGCAACCCCAGGCAGAGGCCAAAGTGAATGGCACAACGGTGTCCTCT
CCTTCTACCTCTCTACAGAGGTCCGACAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCGGACTCCATGGGTGAGGAAGATCTTCTCA
GTCCTCCCCAGGACACAAGCACAGGGTTAGAGGAGGTGATGGAGCAACTCAACAACTCCTTCCCTAGTTCAAGAGGAAGAAATACCCCTGGAAAGCCAAT
GAGAGAGGACACAATGTAGGAAGTCTTTTCCACATGGCAGATGATTTGGGCAGAGCGATGGAGTCCTTAGTATCAGTCATGACAGATGAAGAAGGAGCAG
AATAAATGTTTTACAACTCCTGATTCCCGCATGGTTTTTATAATATTCATACAACAAAGAGGATTAGACAGTAAGAGTTTACAAGAAATAAATCTATATT
TTTGTGAAGGGTAGTGGTATTATACTGTAGATTTCAGTAGTTTCTAAGTCTGTTATTGTTTTGTTAACAATGGCAGGTTTTACACGTCTATGCAATTGTA
CAAAAAAGTTATAAGAAAACTACATGTAAAATCTTGATAGCTAAATAACTTGCCATTTCTTTATATGGAACGCATTTTGGGTTGTTTAAAAATTTATAAC
AGTTATAAAGAAAGATTGTAAACTAAAGTGTGCTTTATAAAAAAAAGTTGTTTATAAAAACCCCTAAAAACAAAACAAACACACACACACACACATACAC
ACACACACACAAAACTTTGAGGCAGCGCATTGTTTTGCATCCTTTTGGCGTGATATCCATATGAAATTCATGGCTTTTTCTTTTTTTGCATATTAAAGAT
AAGACTTCCTCTACCACCACACCAAATGACTACTACACACTGCTCATTTGAGAACTGTCAGCTGAGTGGGGCAGGCTTGAGTTTTCATTTCATATATCTA
TATGTCTATAAGTATATAAATACTATAGTTATATAGATAAAGAGATACGAATTTCTATAGACTGACTTTTTCCATTTTTTAAATGTTCATGTCACATCCT
AATAGAAAGAAATTACTTCTAGTCAGTCATCCAGGCTTACCTGCTTGGTCTAGAATGGATTTTTCCCGGAGCCGGAAGCCAGGAGGAAACTACACCACAC
TAAAACATTGTCTACAGCTCCAGATGTTTCTCATTTTAAACAACTTTCCACTGACAACGAAAGTAAAGTAAAGTATTGGATTTTTTTAAAGGGAACATGT
GAATGAATACACAGGACTTATTATATCAGAGTGAGTAATCGGTTGGTTGGTTGATTGATTGATTGATTGATACATTCAGCTTCCTGCTGCTAGCAATGCC
ACGATTTAGATTTAATGATGCTTCAGTGGAAATCAATCAGAAGGTATTCTGACCTTGTGAACATCAGAAGGTATTTTTTAACTCCCAAGCAGTAGCAGGA
CGATGATAGGGCTGGAGGGCTATGGATTCCCAGCCCATCCCTGTGAAGGAGTAGGCCACTCTTTAAGTGAAGGATTGGATGATTGTTCATAATACATAAA
GTTCTCTGTAATTACAACTAAATTATTATGCCCTCTTCTCACAGTCAAAAGGAACTGGGTGGTTTGGTTTTTGTTGCTTTTTTAGATTTATTGTCCCATG
TGGGATGAGTTTTTAAATGCCACAAGACATAATTTAAAATAAATAAACTTTGGGAAAAGGTGTAAAACAGTAGCCCCATCACATTTGTGATACTGACAGG
TATCAACCCAGAAGCCCATGAACTGTGTTTCCATCCTTTGCATTTCTCTGCGAGTAGTTCCACACAGGTTTGTAAGTAAGTAAGAAAGAAGGCAAATTGA
TTCAAATGTTACAAAAAAACCCTTCTTGGTGGATTAGACAGGTTAAATATATAAACAAACAAACAAAAATTGCTCAAAAAAGAGGAGAAAAGCTCAAGAG
GAAAAGCTAAGGACTGGTAGGAAAAAGCTTTACTCTTTCATGCCATTTTATTTCTTTTTGATTTTTAAATCATTCATTCAATAGATACCACCGTGTGACC
TATAATTTTGCAAATCTGTTACCTCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGCTGACATCAAGTGTAATTAGCTTTTGGAGAGTGGGTTTTGT
CCATTATTAATAATTAATTAATTAACATCAAACACGGCTTCTCATGCTATTTCTACCTCACTTTGGTTTTGGGGTGTTCCTGATAATTGTGCACACCTGA
GTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTTTTTCCTTTATAATTCTTTCTTTTTCCTTCATAATTTTCAAAAGAAAACCCAAAGCTCTAAG
GTAACAAATTACCAAATTACATGAAGATTTGGTTTTTGTCTTGCATTTTTTTCCTTTATGTGACGCTGGACCTTTTCTTTACCCAAGGATTTTTAAAACT
CAGATTTAAAACAAGGGGTTACTTTACATCCTACTAAGAAGTTTAAGTAAGTAAGTTTCATTCTAAAATCAGAGGTAAATAGAGTGCATAAATAATTTTG
TTTTAATCTTTTTGTTTTTCTTTTAGACACATTAGCTCTGGAGTGAGTCTGTCATAATATTTGAACAAAAATTGAGAGCTTTATTGCTGCATTTTAAGCA
TAATTAATTTGGACATTATTTCGTGTTGTGTTCTTTATAACCACCAAGTATTAAACTGTAAATCATAATGTAACTGAAGCATAAACATCACATGGCATGT
TTTGTCATTGTTTTCAGGTACTGAGTTCTTACTTGAGTATCATAATATATTGTGTTTTAACACCAACACTGTAACATTTACGAATTATTTTTTTAAACTT
CAGTTTTACTGCATTTTCACAACATATCAGACTTCACCAAATATATGCCTTACTATTGTATTATAGTACTGCTTTACTGTGTATCTCAATAAAGCACGCA
GTTATGTTAC
- refseq_DMD.F7 (forward primer)
- refseq_DMD.R7 (reverse primer)
- Single exon skipping
Frame 0
TLAMAKLCAEALLAALN*KHPWGPKEVFAHLIKELQDGIGQRQTVVRTLNATGEEIIQQSSKTDASI
LQEKLGSLNLRWQEVCKQLSDRKKRLEEQKNIL
SEFQRDLNEFVLWLEEADNIASIPLEPGKEQQL
KEKLEQVKLLVEELPLRQGILKQLNETGGPVLVS
APISPEEQDKLENKLKQTNLQWIKVSRALPEKQ
GEIEAQIKDLGQLEKKLEDLEEQLNHLLLWLSP
IRNQLEIYNQPNQEGPFDVKETEIAVQAKQPDVE
EILSKGQHLYKEKPATQPVKRKLEDLSSEWKAV
NRLLQELRAKQPDLAPGLTTIGASPTQTVTLVT
QPVVTKETAISKLEMPSSLMLEVPALADFNRAWT
ELTDWLSLLDQVIKSQRVMVGDLEDINEMIIKQ
KATMQDLEQRRPQLEELITAAQNLKNKTSNQEA
RTIITDRIERIQNQWDEVQEHLQNRRQQLNEMLK
DSTQWLEAKEEAEQVLGQARAKLESWKEGPYTV
DAIQKKITETKQLAKDLRQWQTNVDVANDLALK
LLRDYSADDTRKVHMITENINASWRSIHKRVSER
EAALEETHRLLQQFPLDLEKFLAWLTEAETTAN
VLQDATRKERLLEDSKGVKELMKQWQDLQGEIE
AHTDVYHNLDENSQKILRSLEGSDDAVLLQRRLD
NMNFKWSELRKKSLNIRSHLEASSDQWKRLHLS
LQELLVWLQLKDDELSRQAPIGGDFPAVQKQND
VHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEG
LEKLYQEPRELPPEERAQNVTRLLRKQAEEVNT
EWEKLNLHSADWQRKIDETLERLRELQEATDEL
DLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKA
LRGEIAPLKENVSHVNDLARQLTTLGIQLSPYN
LSTLEDLNTRWKLLQVAVEDRVRQLHEAHRDFG
PASQHFLSTSVQGPWERAISPNKVPYYINHETQT
TCWDHPKMTELYQSLADLNNVRFSAYRTAMKLR
RLQKALCLDLLSLSAACDALDQHNLKQNDQPMD
ILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLN
WLLNVYDTGRTGRIRVLSFKTGIISLCKAHLED
KYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPR
QLGEVASFGGSNIEPSVRSCFQFANNKPEIEAAL
FLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKC
NICKECPIIGFRYRSLKHFNYDICQSCFFSGRV
AKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKF
RTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLI
NFWPVDSAPASSPQLSHDDTHSRIEHYASRLAE
MENSNGSYLNDSISPNESIDDEHLLIQHYCQSLN
QDSPLSQPRSPAQILISLESEERGELERILADL
EEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMM
PTSPQSPRDAELIAEAKLLRQHKGRLEARMQILE
DHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSS
PSTSLQRSDSSQPMLLRVVGSQTSDSMGEEDLL
SPPQDTSTGLEEVMEQLNNSFPSSRGRNTPGKPM
REDTM*EVFSTWQMIWAERWSP*YQS*QMKKEQ
NKCFTTPDSRMVFIIFIQQRGLDSKSLQEINLY
FCEG*WYYTVDFSSF*VCYCFVNNGRFYTSMQLY
KKVIRKLHVKS**LNNLPFLYMERILGCLKIYN
SYKERL*TKVCFIKKSCL*KPLKTKQTHTHTHT
HTHTKL*GSALFCILLA*YPYEIHGFFFFCILKI
RLPLPPHQMTTTHCSFENCQLSGAGLSFHFIYL
YVYKYINTIVI*IKRYEFL*TDFFHFLNVHVTS
**KEITSSQSSRLTCLV*NGFFPEPEARRKLHHT
KTLSTAPDVSHFKQLSTDNESKVKYWIFLKGTC
E*IHRTYYIRVSNRLVG*LID*LIHSASCC*QC
HDLDLMMLQWKSIRRYSDLVNIRRYFLTPKQ*QD
DDRAGGLWIPSPSL*RSRPLFK*RIG*LFIIHK
VLCNYN*IIMPSSHSQKELGGLVFVAFLDLLSH
VG*VFKCHKT*FKINKLWEKV*NSSPITFVILTG
INPEAHELCFHPLHFSASSSTQVCK*VRKKAN*
FKCYKKTLLGGLDRLNI*TNKQKLLKKEEKSSR
GKAKDW*EKALLFHAILFLFDF*IIHSIDTTV*P
IILQICYL*HQV*LAFGEWADIKCN*LLESGFC
PLLIIN*LTSNTASHAISTSLWFWGVPDNCAHL
SSQLHHLSIALFSFSFIILSFSFIIFKRKPKALR
*QITKLHEDLVFVLHFFPLCDAGPFLYPRIFKT
QI*NKGLLYILLRSLSK*VSF*NQR*IECINNF
VLIFLFFF*TH*LWSESVIIFEQKLRALLLHFKH
N*FGHYFVLCSL*PPSIKL*IIM*LKHKHHMAC
FVIVFRY*VLT*VS*YIVF*HQHCNIYELFF*T
SVLLHFHNISDFTKYMPYYCIIVLLYCVSQ*STQ
LCY
Frame 1
H*QWQSFVRRHCWLL*TKSIRGDRKRFLHTLLRNSRMALGSGKLLSEH*MQLGKK*FSNPQKQMPV
FYRKNWEA*ICGGRRSANSCQTEKRG*KNKRISC
QNFKEI*MNLFYGWRKQITLLVSHLNLEKSSN*
KKSLSKSSYWWKSCPCAREFSNN*MKLEDPCL*
VLP*AQKSKINLKISSSRQISSG*RFPELYLRNK
EKLKLK*KTLGSLKKSLKTLKSS*IICCCGYLL
LGISWKFITNQTKKDHLTLRKLK*QFKLNNRMW
KRFCLKGSICTRKNQPLSQ*RGS*KI*ALSGRR*
TVYFKS*GQSSLT*LLD*PLLEPLLLRLLLW*H
NLWLLRKLPSPN*KCHLP*CWRYLLWQISTGLG
QNLPTGFLCLIKL*NHRG*WWVTLRISTR*SSSR
RQQCRIWNRGVPSWKNSLPLPKI*KTRPAIKRL
EQSLRIELKEFRISGMKYKNTFRTGGNS*MKC*
RIQHNGWKLRKKLSRS*DRPEPSLSHGRRVPIQ*
MQSKRKSQKPSSWPKTSASGRQM*MWQMTWP*N
FSGIILQMIPEKST**QRISMPLGEAFIKG*VS
ERLLWKKLIDYCNSSPWTWKSFLPGLQKLKQLPM
SYRMLPVRKGS*KTPRE*KS**NNGKTSKVKLK
LTQMFITTWMKTAKKS*DPWKVPMMQSCYKDVW
IT*TSSGVNFGKSLSTLGPIWKPVLTSGSVCTFL
CRNFWCGYS*KMMN*AGRHLLEATFQQFRSRTM
YIGPSRGN*KLKNL*S*VLLRLYEYF*QSSLWK
D*RNSTRSPESCLLRREPRMSLGFYESRLRRSIL
SGKN*TCTPLTGREK*MRPLKDSGNFKRPRMSW
TSSCAKLR*SRDPGSPWAISSLTLSKITSRKSR
HFEEKLRL*KRT*ATSMTLLASLPLWAFSSHRIT
SALWKT*TPDGSFCRWPSRTESGSCMKPTGTLV
QHLSTFFPRLSRVPGREPSRQTKCPTISTTRLK
QLAGTIPK*QSSTSL*LT*IMSDSQLIGLP*NSE
DCRRPFAWIS*ACQLHVMPWTSTTSSKMTSPWI
SCRLLIV*PLFMTAWSKSTTIWSTSLSAWICV*
TGC*MFMIRDEQGGSVSCLLKLASFPCVKHIWKT
STDTFSSKWQVQQDFVTSAGWASFCMILSKFQD
SWVKLHPLGAVTLSQVSGAASNLLIISQRSKRP
SS*TG*DWNPSPWCGCPSCTEWLLQKLPSIRPNV
TSAKSVQSLDSGTGV*STLIMTSAKAAFFLVEL
QKAIKCTIPWWNIALRLHQEKMFETLPRY*KTN
FEPKGILRSIPEWATCQCRLS*RGTTWKLPLL*S
TSGQ*ILRLPRPLSFHTMILIHALNIMLAG*QK
WKTAMDLI*MIASLLMRA*MMNIC*SSITAKV*
TRTPP*ASLVVLPRS*FP*RVRKEGS*RES*QIL
RKKTGICKQNMTV*SSSTNIKACPHCRPLLK*C
PPLPRVPGMLSSLLRPSYCVNTKAAWKPGCKSW
KTTINSWSHSYTG*GSCWSNPRQRPK*MAQRCPL
LLPLYRGPTAVSLCCSEWLAVKLRTPWVRKIFS
VLPRTQAQG*RR*WSNSTTPSLVQEEEIPLESQ
*ERTQCRKSFPHGR*FGQSDGVLSISHDR*RRSR
INVLQLLIPAWFL*YSYNKED*TVRVYKK*IYI
FVKGSGIIL*ISVVSKSVIVLLTMAGFTRLCNC
TKKL*ENYM*NLDS*ITCHFFIWNAFWVV*KFIT
VIKKDCKLKCAL*KKVVYKNP*KQNKHTHTHIH
THTQNFEAAHCFASFWRDIHMKFMAFSFFAY*R
*DFLYHHTK*LLHTAHLRTVS*VGQA*VFISYIY
MSISI*IL*LYR*RDTNFYRLTFSIF*MFMSHP
NRKKLLLVSHPGLPAWSRMDFSRSRKPGGNYTT
LKHCLQLQMFLILNNFPLTTKVK*SIGFF*REHV
NEYTGLIISE*VIGWLVD*LID*YIQLPAASNA
TI*I**CFSGNQSEGILTL*TSEGIF*LPSSSR
TMIGLEGYGFPAHPCEGVGHSLSEGLDDCS*YIK
FSVITTKLLCPLLTVKRNWVVWFLLLF*IYCPM
WDEFLNATRHNLK*INFGKRCKTVAPSHL*Y*Q
VSTQKPMNCVSILCISLRVVPHRFVSK*ERRQID
SNVTKKPFLVD*TG*IYKQTNKNCSKKRRKAQE
EKLRTGRKKLYSFMPFYFFLIFKSFIQ*IPPCD
L*FCKSVTSDIKCN*LLESGLTSSVISFWRVGFV
HY**LIN*HQTRLLMLFLPHFGFGVFLIIVHT*
VHSFTTCPLRYFLFPL*FFLFPS*FSKENPKL*
GNKLPNYMKIWFLSCIFFLYVTLDLFFTQGFLKL
RFKTRGYFTSY*EV*VSKFHSKIRGK*SA*IIL
F*SFCFSFRHISSGVSLS*YLNKN*ELYCCILS
IINLDIISCCVLYNHQVLNCKS*CN*SINITWHV
LSLFSGTEFLLEYHNILCFNTNTVTFTNYFFKL
QFYCIFTTYQTSPNICLTIVL*YCFTVYLNKAR
SYV
Frame 2
TSNGKALCGGIAGCSELKASVGTERGFCTPY*GTPGWHWAAANCCQNIECNWGRNNSAILKNRCQY
STGKIGKPESAVAGGLQTAVRQKKEARRTKEYL
VRISKRFK*ICFMVGGSR*HC*YPT*TWKRAATK
RKA*ASQVTGGRVAPAPGNSQTIK*NWRTRACK
CSHKPRRAR*T*K*AQADKSPVDKGFQSFT*ET
RRN*SSNKRPWAA*KKA*RP*RAVKSSAAVVISY
*ESVGNL*PTKPRRTI*R*GN*NSSSS*TTGCG
RDFV*RAAFVQGKTSHSASEEEVRRSEL*VEGG
KPFTSRAEGKAA*PSSWTDHYWSLSYSDCYSGDT
TCGY*GNCHLQTRNAIFLDVGGTCSGRFQPGLD
RTYRLAFSA*SSYKITEGDGG*P*GYQRDDHQA
EGNNAGFGTEASPVGRTHYRCPKFEKQDQQSRG*
NNHYGSN*KNSESVG*STRTPSEPEATVE*NVK
GFNTMAGS*GRS*AGLRTGQSQA*VMEGGSLYS
RCNPKENHRNQAVGQRPPPVADKCRCGK*LGPET
SPGLFCR*YQKSPHDNREYQCLLEKHS*KGE*A
RGCFGRNS*ITATVPPGPGKVSCLAYRS*NNCQ
CPTGCYP*GKAPRRLQGSKRADETMARPPR*N*S
SHRCLSQPG*KQPKNPEIPGRFR*CSPVTKTFG
*HELQVE*TSEKVSQH*VPFGSQF*PVEASAPF
SAGTSGVATAER**IKPAGTYWRRLSSSSEAERC
T*GLQEGIEN*RTCNHEYS*DCTNISDRAAFGR
TRETLPGAQRAAS*GESPECHSASTKAG*GGQY
*VGKIEPALR*LAEKNR*DP*KTPGTSRGHG*AG
PQAAPS*GDQGILAARGRSPH*LSPRSPRESQG
TSRRNCASEREREPRQ*PCSPAYHFGHSALTV*
PQHSGRPEHQMEASAGGRRGPSQAAA*SPQGLWS
SISALSFHVCPGSLGESHLAKQSALLYQPRDSN
NLLGPSQNDRALPVFS*PE*CQILSL*DCHETP
KTAEGPLLGSLEPVSCM*CLGPAQPQAK*PAHGY
PADY*LFDHYL*PPGARAQQFGQRPSLRGYVSE
LAAECL*YGTNREDPCPVF*NWHHFPV*STFGR
QVQIPFQASGKFNRIL*PAQAGPPSA*FYPNSKT
VG*SCILWGQ*H*AKCPELLPIC***ARDRSGP
LPRLDETGTPVHGVAARPAQSGCCRNCQASGQM
*HLQRVSNHWIQVQESKAL*L*HLPKLLFFWSSC
KRP*NALSHGGILHSDYIRRRCSRLCQGTKKQI
SNQKVFCEASPNGLPASADCLRGGQHGNSRYSD
QLLASRFCACLVPSAFTR*YSFTH*TLC*QASRN
GKQQWILSK**HLS**EHR**TFVNPALLPKFE
PGLPPEPAS*SCPDLDFLRE*GKRGARENPSRS
*GRKQESASRI*PSKAAART*RPVPTAVPS*NDA
HLSPESPGC*AHC*GQATASTQRPPGSQDANPG
RPQ*TAGVTVTQAKAAAGATPGRGQSEWHNGVL
SFYLSTEVRQQSAYAAPSGWQSNFGLHG*GRSSQ
SSPGHKHRVRGGDGATQQLLP*FKRKKYPWKAN
ERGHNVGSLFHMADDLGRAMESLVSVMTDEEGA
E*MFYNS*FPHGFYNIHTTKRIRQ*EFTRNKSIF
L*RVVVLYCRFQ*FLSLLLFC*QWQVLHVYAIV
QKSYKKTTCKILIAK*LAISLYGTHFGLFKNL*
QL*RKIVN*SVLYKKKLFIKTPKNKTNTHTHTYT
HTHKTLRQRIVLHPFGVISI*NSWLFLFLHIKD
KTSSTTTPNDYYTLLI*ELSAEWGRLEFSFHIS
ICL*VYKYYSYIDKEIRISID*LFPFFKCSCHIL
IERNYF*SVIQAYLLGLEWIFPGAGSQEETTPH
*NIVYSSRCFSF*TTFH*QRK*SKVLDFFKGNM
*MNTQDLLYQSE*SVGWLID*LIDTFSFLLLAMP
RFRFNDASVEINQKVF*PCEHQKVFFNSQAVAG
R**GWRAMDSQPIPVKE*ATL*VKDWMIVHNT*
SSL*LQLNYYALFSQSKGTGWFGFCCFFRFIVPC
GMSF*MPQDII*NK*TLGKGVKQ*PHHICDTDR
YQPRSP*TVFPSFAFLCE*FHTGL*VSKKEGKL
IQMLQKNPSWWIRQVKYINKQTKIAQKRGEKLKR
KS*GLVGKSFTLSCHFISF*FLNHSFNRYHRVT
YNFANLLPLTSSVISFWRVG*HQV*LAFGEWVL
SIINN*LINIKHGFSCYFYLTLVLGCS**LCTPE
FTASPLVHCVIFFFLYNSFFFLHNFQKKTQSSK
VTNYQIT*RFGFCLAFFSFM*RWTFSLPKDF*N
SDLKQGVTLHPTKKFK*VSFILKSEVNRVHK*FC
FNLFVFLLDTLALE*VCHNI*TKIESFIAAF*A
*LIWTLFRVVFFITTKY*TVNHNVTEA*TSHGM
FCHCFQVLSSYLSIIIYCVLTPTL*HLRIIFLNF
SFTAFSQHIRLHQIYALLLYYSTALLCISIKHA
VML
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