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Sequences

Sequence NM_001001567

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGAATTCAGAACGCACTCCGTACAAAGTGAGA
CCTGTGGCCATCAAGCAACTCTCCGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGAAC
TGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAA
GAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGAT
GTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGA
GCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTA
CAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAA
ACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGG
TCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACC
TGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATG
GAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCG
CAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAA
AGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATG
CTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTG
GGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGG
CTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACA
AAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F2 (forward primer)
  • refseq_PDE9A.R2 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RNTTISLLTTDDAMVSIDPTMPANSERTPYKVR
PVAIKQLSEREELIQSVLAQVAEQFSRAFKINE
LKAEVANHLAVLEKRVELEGLKVVEIEKCKSDIK
KMREELAARSSRTNCPCKYSFLDNHKKLTPRRD
VPTYPKYLLSPETIEALRKPTFDVWLWEPNEML
SCLEHMYHDLGLVRDFSINPVTLRRWLFCVHDNY
RNNPFHNFRHCFCVAQMMYSMVWLCSLQEKFSQ
TDILILMTAAICHDLDHPGYNNTYQINARTELA
VRYNDISPLENHHCAVAFQILAEPECNIFSNIPP
DGFKQIRQGMITLILATDMARHAEIMDSFKEKM
ENFDYSNEEHMTLLKMILIKCCDISNEVRPMEV
AEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDK
VTKATAQIGFIKFVLIPMFETVTKLFPMVEEIM
LQPLWESRDRYEELKRIDDAMKELQKKTDSLTS
GATEKSRERSRDVKNSEGDCA*GKRGAWLQFWTG
WPSCAGSLCREELPWAPGTTRPCFLRTILFTDT
KKKKGIHDAVQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LGTRPSPC*PPTTPWSPSTPPCPRIQNALRTK*D
LWPSSNSPREKN*SRACWRRLQSSSQEHSKSMN
*KLKLQITWLS*RNAWNWKD*KWWRLRNARVTL
RR*GRSWRPEAAGPTAPVSTVFWITTRS*LLDAM
FPLTPSTCSLQRPSRPCGSRPLTSGFGSPMRC*
AAWSTCTTTSGWSGTSASTLSPSGGGCSASTTT
TETTPSTTSGTASAWPR*CTAWSGSAVSRRSSHK
RIS*S**QRPSATIWTIPATTTRTRSMPAQSWR
SATMTSHRWRTTTAPWPSRSSPSLSATSSPTSH
LMGSSRSDRE*SH*SWPLTWQDMQKLWILSKRKW
RILTTATRST*PC*R*F**NAVISLTRSVQWKS
QSLGWTVY*RNILCRATVRSQKAFLWHRSWTET
K*PRPQPRLGSSSLS*SQCLKQ*PSSSPWLRRSC
CSHFGNPEIATRS*SG*MTP*KSYRRRLTA*RL
GPPRSPEREAEM*KTVKETVPEESGGRGCSSGR
AGRAARDPCAGKSCPGHLAPQDHVF*EPFCSLIQ
KKKKEFMMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SEHDHLPADHRRRHGLHRPHHAREFRTHSVQSE
TCGHQATLRERRINPERAGAGCRAVLKSIQNQ*T
ES*SCKSLGCPRETRGIGRTKSGGD*EMQE*H*
EDEGGAGGQKQQDQLPL*VQFFG*PQEVDSSTR
CSHLPQVPALSRDHRGPAEADL*RLALGAQ*DAE
LPGAHVPRPRAGQGLQHQPCHPQEVAVLRPRQL
QKQPLPQLPALLLRGPDDVQHGLALQSPGEVLT
NGYPDPNDSGHLPRSGPSRLQQHVPDQCPHRAGG
PLQ*HLTAGEPPLRRGLPDPRRA*VQHLLQHPT
*WVQADPTGNDHINLGH*HGKTCRNYGFFQREN
GEF*LQQRGAHDPAEDDFDKML*YL*RGPSNGSR
RALGGLFIRGIFYAERP*EVRRPSCGTVHGPRQ
SDQGHSPDWVHQVCPDPNV*NSDQALPHG*GDH
AAATLGIPRSLRGAEADR*RHERVTEED*QLDVW
GHREVQREKQRCEKQ*RRLCLRKAGGVAAVLDG
LAELRGILVQGRAALGTWHHKTMFSKNHFVH*Y
KKKKRNS*CCTEFYF*TVF*IIYSYTE

Sequence NM_001001571

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCA
GTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTG
GAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATA
ACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGT
CTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGG
AGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGC
TCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTA
CCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCT
GAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAG
AAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATAT
CTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTT
CCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCA
AGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTT
ACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAG
CGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTT
TTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCT
TATACGGAAA
  • refseq_PDE9A.F2 (forward primer)
  • refseq_PDE9A.R2 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTHSVQSETCGHQATLRERRINPERAGAGCRA
VLKSIQNQ*TES*SCKSLGCPRETRGIGRTKSG
GD*EMQE*H*EDEGGAGGQKQQDQLPL*VQFFG*
PQEVDSSTRCSHLPQVPALSRDHRGPAEADL*R
LALGAQ*DAELPGAHVPRPRAGQGLQHQPCHPQ
EVAVLRPRQLQKQPLPQLPALLLRGPDDVQHGLA
LQSPGEVLTNGYPDPNDSGHLPRSGPSRLQQHV
PDQCPHRAGGPLQ*HLTAGEPPLRRGLPDPRRA
*VQHLLQHPT*WVQADPTGNDHINLGH*HGKTCR
NYGFFQRENGEF*LQQRGAHDPAEDDFDKML*Y
L*RGPSNGSRRALGGLFIRGIFYAERP*EVRRP
SCGTVHGPRQSDQGHSPDWVHQVCPDPNV*NSDQ
ALPHG*GDHAAATLGIPRSLRGAEADR*RHERV
TEED*QLDVWGHREVQREKQRCEKQ*RRLCLRK
AGGVAAVLDGLAELRGILVQGRAALGTWHHKTMF
SKNHFVH*YKKKKRNS*CCTEFYF*TVF*IIYS
YTE

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERTPYKVRPVAIKQLSEREELIQSVLAQVAEQ
FSRAFKINELKAEVANHLAVLEKRVELEGLKVV
EIEKCKSDIKKMREELAARSSRTNCPCKYSFLD
NHKKLTPRRDVPTYPKYLLSPETIEALRKPTFDV
WLWEPNEMLSCLEHMYHDLGLVRDFSINPVTLR
RWLFCVHDNYRNNPFHNFRHCFCVAQMMYSMVW
LCSLQEKFSQTDILILMTAAICHDLDHPGYNNTY
QINARTELAVRYNDISPLENHHCAVAFQILAEP
ECNIFSNIPPDGFKQIRQGMITLILATDMARHA
EIMDSFKEKMENFDYSNEEHMTLLKMILIKCCDI
SNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGL
PVAPFMDRDKVTKATAQIGFIKFVLIPMFETVT
KLFPMVEEIMLQPLWESRDRYEELKRIDDAMKEL
QKKTDSLTSGATEKSRERSRDVKNSEGDCA*GK
RGAWLQFWTGWPSCAGSLCREELPWAPGTTRPC
FLRTILFTDTKKKKGIHDAVQNFIFKLSFK*YIL
IRK

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNALRTK*DLWPSSNSPREKN*SRACWRRLQS
SSQEHSKSMN*KLKLQITWLS*RNAWNWKD*KWW
RLRNARVTLRR*GRSWRPEAAGPTAPVSTVFWI
TTRS*LLDAMFPLTPSTCSLQRPSRPCGSRPLT
SGFGSPMRC*AAWSTCTTTSGWSGTSASTLSPSG
GGCSASTTTTETTPSTTSGTASAWPR*CTAWSG
SAVSRRSSHKRIS*S**QRPSATIWTIPATTTR
TRSMPAQSWRSATMTSHRWRTTTAPWPSRSSPSL
SATSSPTSHLMGSSRSDRE*SH*SWPLTWQDMQ
KLWILSKRKWRILTTATRST*PC*R*F**NAVI
SLTRSVQWKSQSLGWTVY*RNILCRATVRSQKAF
LWHRSWTETK*PRPQPRLGSSSLS*SQCLKQ*P
SSSPWLRRSCCSHFGNPEIATRS*SG*MTP*KS
YRRRLTA*RLGPPRSPEREAEM*KTVKETVPEES
GGRGCSSGRAGRAARDPCAGKSCPGHLAPQDHV
F*EPFCSLIQKKKKEFMMLYRILFLNCLLNNIF
LYG

Sequence NM_002606

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGTAATCTTCAGCAAGTACTGCAACTCCAGCGACATCATGGACCTGTTCTGCATCGCCACCGGCCTGCC
TCGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGAATTCAGAACGCACTCCGTACAAAGTGAGA
CCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGAGAGACCACTGAGGGACAGACGGGTTGTGG
GCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAGGGCTGCTACCAGGAAGGCCAGCGCATCCC
TCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAATCAATGAACTGAAAGCTGAAGTTGCAAAT
CACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAGTGACATTAAGAAGATGAGGGAGGAGCTGG
CGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCTCGACGCGATGTTCCCACTTACCCCAAGTA
CCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATGAGATGCTGAGCTGCCTGGAGCACATGTAC
CACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCACGACAACTACAGAAACAACCCCTTCCACA
ACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAGTTCTCACAAACGGATATCCTGATCCTAAT
GACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAGAGCTGGCGGTCCGCTACAATGACATCTCA
CCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAACATCCCACCTGATGGGTTCAAGCAGATCC
GACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAAGAGAAAATGGAGAATTTTGACTACAGCAA
CGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAATGGAAGTCGCAGAGCCTTGGGTGGACTGT
TTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGACCGAGACAAAGTGACCAAGGCCACAGCCC
AGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAGGAGATCATGCTGCAGCCACTTTGGGAATC
CCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCTTGACGTCTGGGGCCACCGAGAAGTCCAGA
GAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTTCTGGACGGGCTGGCCGAGCTGCGCGGGAT
CCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTCACTGATACAAAAAAAAAAAAAGGAATTCA
TGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F2 (forward primer)
  • refseq_PDE9A.R2 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKVIFSKYCNSSDIMDLFCIATGLP
RNTTISLLTTDDAMVSIDPTMPANSERTPYKVR
PVAIKQLSAGVEDKRTTSRGQSAERPLRDRRVV
GLEQPRREGAFESGQVEPRPREPQGCYQEGQRIP
PEREELIQSVLAQVAEQFSRAFKINELKAEVAN
HLAVLEKRVELEGLKVVEIEKCKSDIKKMREEL
AARSSRTNCPCKYSFLDNHKKLTPRRDVPTYPKY
LLSPETIEALRKPTFDVWLWEPNEMLSCLEHMY
HDLGLVRDFSINPVTLRRWLFCVHDNYRNNPFH
NFRHCFCVAQMMYSMVWLCSLQEKFSQTDILILM
TAAICHDLDHPGYNNTYQINARTELAVRYNDIS
PLENHHCAVAFQILAEPECNIFSNIPPDGFKQI
RQGMITLILATDMARHAEIMDSFKEKMENFDYSN
EEHMTLLKMILIKCCDISNEVRPMEVAEPWVDC
LLEEYFMQSDREKSEGLPVAPFMDRDKVTKATA
QIGFIKFVLIPMFETVTKLFPMVEEIMLQPLWES
RDRYEELKRIDDAMKELQKKTDSLTSGATEKSR
ERSRDVKNSEGDCA*GKRGAWLQFWTGWPSCAG
SLCREELPWAPGTTRPCFLRTILFTDTKKKKGIH
DAVQNFIFKLSFK*YILIRK

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRR*SSASTATPATSWTCSASPPAC
LGTRPSPC*PPTTPWSPSTPPCPRIQNALRTK*D
LWPSSNSPLVSRTREPQAVASLLRDH*GTDGLW
AWSSPGGKEHLKVDR*SPGPESPRAATRKASAS
LQREKN*SRACWRRLQSSSQEHSKSMN*KLKLQI
TWLS*RNAWNWKD*KWWRLRNARVTLRR*GRSW
RPEAAGPTAPVSTVFWITTRS*LLDAMFPLTPS
TCSLQRPSRPCGSRPLTSGFGSPMRC*AAWSTCT
TTSGWSGTSASTLSPSGGGCSASTTTTETTPST
TSGTASAWPR*CTAWSGSAVSRRSSHKRIS*S*
*QRPSATIWTIPATTTRTRSMPAQSWRSATMTSH
RWRTTTAPWPSRSSPSLSATSSPTSHLMGSSRS
DRE*SH*SWPLTWQDMQKLWILSKRKWRILTTA
TRST*PC*R*F**NAVISLTRSVQWKSQSLGWTV
Y*RNILCRATVRSQKAFLWHRSWTETK*PRPQP
RLGSSSLS*SQCLKQ*PSSSPWLRRSCCSHFGN
PEIATRS*SG*MTP*KSYRRRLTA*RLGPPRSPE
REAEM*KTVKETVPEESGGRGCSSGRAGRAARD
PCAGKSCPGHLAPQDHVF*EPFCSLIQKKKKEF
MMLYRILFLNCLLNNIFLYG

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGNLQQVLQLQRHHGPVLHRHRPA
SEHDHLPADHRRRHGLHRPHHAREFRTHSVQSE
TCGHQATLRWCRGQENHKPWPVC*ETTEGQTGCG
PGAAPEGRSI*KWTGRAQAQRAPGLLPGRPAHP
SRERRINPERAGAGCRAVLKSIQNQ*TES*SCK
SLGCPRETRGIGRTKSGGD*EMQE*H*EDEGGAG
GQKQQDQLPL*VQFFG*PQEVDSSTRCSHLPQV
PALSRDHRGPAEADL*RLALGAQ*DAELPGAHV
PRPRAGQGLQHQPCHPQEVAVLRPRQLQKQPLPQ
LPALLLRGPDDVQHGLALQSPGEVLTNGYPDPN
DSGHLPRSGPSRLQQHVPDQCPHRAGGPLQ*HL
TAGEPPLRRGLPDPRRA*VQHLLQHPT*WVQADP
TGNDHINLGH*HGKTCRNYGFFQRENGEF*LQQ
RGAHDPAEDDFDKML*YL*RGPSNGSRRALGGL
FIRGIFYAERP*EVRRPSCGTVHGPRQSDQGHSP
DWVHQVCPDPNV*NSDQALPHG*GDHAAATLGI
PRSLRGAEADR*RHERVTEED*QLDVWGHREVQ
REKQRCEKQ*RRLCLRKAGGVAAVLDGLAELRGI
LVQGRAALGTWHHKTMFSKNHFVH*YKKKKRNS
*CCTEFYF*TVF*IIYSYTE

Sequence NM_001001582

TCCCGCGGCGGCTGGCGTCGGGAAAGTACAGTAAAAAGTCCGAGTGCAGCCGCCGGGCGCAGGATGGGATCCGGCTCCTCCAGCTACCGGCCCAAGGCCA
TCTACCTGGACATCGATGGACGCATTCAGAAGGAACACGACCATCTCCCTGCTGACCACCGACGACGCCATGGTCTCCATCGACCCCACCATGCCCGCGA
ATTCAGAACGCACTCCGTACAAAGTGAGACCTGTGGCCATCAAGCAACTCTCCGCTGGTGTCGAGGACAAGAGAACCACAAGCCGTGGCCAGTCTGCTGA
GAGACCACTGAGGGACAGACGGGTTGTGGGCCTGGAGCAGCCCCGGAGGGAAGGAGCATTTGAAAGTGGACAGGTAGAGCCCAGGCCCAGAGAGCCCCAG
GGCTGCTACCAGGAAGGCCAGCGCATCCCTCCAGAGAGAGAAGAATTAATCCAGAGCGTGCTGGCGCAGGTTGCAGAGCAGTTCTCAAGAGCATTCAAAA
TCAATGAACTGAAAGCTGAAGTTGCAAATCACTTGGCTGTCCTAGAGAAACGCGTGGAATTGGAAGGACTAAAAGTGGTGGAGATTGAGAAATGCAAGAG
TGACATTAAGAAGATGAGGGAGGAGCTGGCGGCCAGAAGCAGCAGGACCAACTGCCCCTGTAAGTACAGTTTTTTGGATAACCACAAGAAGTTGACTCCT
CGACGCGATGTTCCCACTTACCCCAAGTACCTGCTCTCTCCAGAGACCATCGAGGCCCTGCGGAAGCCGACCTTTGACGTCTGGCTTTGGGAGCCCAATG
AGATGCTGAGCTGCCTGGAGCACATGTACCACGACCTCGGGCTGGTCAGGGACTTCAGCATCAACCCTGTCACCCTCAGGAGGTGGCTGTTCTGCGTCCA
CGACAACTACAGAAACAACCCCTTCCACAACTTCCGGCACTGCTTCTGCGTGGCCCAGATGATGTACAGCATGGTCTGGCTCTGCAGTCTCCAGGAGAAG
TTCTCACAAACGGATATCCTGATCCTAATGACAGCGGCCATCTGCCACGATCTGGACCATCCCGGCTACAACAACACGTACCAGATCAATGCCCGCACAG
AGCTGGCGGTCCGCTACAATGACATCTCACCGCTGGAGAACCACCACTGCGCCGTGGCCTTCCAGATCCTCGCCGAGCCTGAGTGCAACATCTTCTCCAA
CATCCCACCTGATGGGTTCAAGCAGATCCGACAGGGAATGATCACATTAATCTTGGCCACTGACATGGCAAGACATGCAGAAATTATGGATTCTTTCAAA
GAGAAAATGGAGAATTTTGACTACAGCAACGAGGAGCACATGACCCTGCTGAAGATGATTTTGATAAAATGCTGTGATATCTCTAACGAGGTCCGTCCAA
TGGAAGTCGCAGAGCCTTGGGTGGACTGTTTATTAGAGGAATATTTTATGCAGAGCGACCGTGAGAAGTCAGAAGGCCTTCCTGTGGCACCGTTCATGGA
CCGAGACAAAGTGACCAAGGCCACAGCCCAGATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGTGACCAAGCTCTTCCCCATGGTTGAG
GAGATCATGCTGCAGCCACTTTGGGAATCCCGAGATCGCTACGAGGAGCTGAAGCGGATAGATGACGCCATGAAAGAGTTACAGAAGAAGACTGACAGCT
TGACGTCTGGGGCCACCGAGAAGTCCAGAGAGAGAAGCAGAGATGTGAAAAACAGTGAAGGAGACTGTGCCTGAGGAAAGCGGGGGGCGTGGCTGCAGTT
CTGGACGGGCTGGCCGAGCTGCGCGGGATCCTTGTGCAGGGAAGAGCTGCCCTGGGCACCTGGCACCACAAGACCATGTTTTCTAAGAACCATTTTGTTC
ACTGATACAAAAAAAAAAAAAGGAATTCATGATGCTGTACAGAATTTTATTTTTAAACTGTCTTTTAAATAATATATTCTTATACGGAAA
  • refseq_PDE9A.F2 (forward primer)
  • refseq_PDE9A.R2 (reverse primer)
  • Single exon skipping

Frame 0

SRGGWRRESTVKSPSAAAGRRMGSGSSSYRPKA
IYLDIDGRIQKEHDHLPADHRRRHGLHRPHHARE
FRTHSVQSETCGHQATLRWCRGQENHKPWPVC*
ETTEGQTGCGPGAAPEGRSI*KWTGRAQAQRAP
GLLPGRPAHPSRERRINPERAGAGCRAVLKSIQN
Q*TES*SCKSLGCPRETRGIGRTKSGGD*EMQE
*H*EDEGGAGGQKQQDQLPL*VQFFG*PQEVDS
STRCSHLPQVPALSRDHRGPAEADL*RLALGAQ*
DAELPGAHVPRPRAGQGLQHQPCHPQEVAVLRP
RQLQKQPLPQLPALLLRGPDDVQHGLALQSPGE
VLTNGYPDPNDSGHLPRSGPSRLQQHVPDQCPHR
AGGPLQ*HLTAGEPPLRRGLPDPRRA*VQHLLQ
HPT*WVQADPTGNDHINLGH*HGKTCRNYGFFQ
RENGEF*LQQRGAHDPAEDDFDKML*YL*RGPSN
GSRRALGGLFIRGIFYAERP*EVRRPSCGTVHG
PRQSDQGHSPDWVHQVCPDPNV*NSDQALPHG*
GDHAAATLGIPRSLRGAEADR*RHERVTEED*QL
DVWGHREVQREKQRCEKQ*RRLCLRKAGGVAAV
LDGLAELRGILVQGRAALGTWHHKTMFSKNHFV
H*YKKKKRNS*CCTEFYF*TVF*IIYSYTE

Frame 1

PAAAGVGKVQ*KVRVQPPGAGWDPAPPATGPRP
STWTSMDAFRRNTTISLLTTDDAMVSIDPTMPA
NSERTPYKVRPVAIKQLSAGVEDKRTTSRGQSAE
RPLRDRRVVGLEQPRREGAFESGQVEPRPREPQ
GCYQEGQRIPPEREELIQSVLAQVAEQFSRAFK
INELKAEVANHLAVLEKRVELEGLKVVEIEKCKS
DIKKMREELAARSSRTNCPCKYSFLDNHKKLTP
RRDVPTYPKYLLSPETIEALRKPTFDVWLWEPN
EMLSCLEHMYHDLGLVRDFSINPVTLRRWLFCVH
DNYRNNPFHNFRHCFCVAQMMYSMVWLCSLQEK
FSQTDILILMTAAICHDLDHPGYNNTYQINART
ELAVRYNDISPLENHHCAVAFQILAEPECNIFSN
IPPDGFKQIRQGMITLILATDMARHAEIMDSFK
EKMENFDYSNEEHMTLLKMILIKCCDISNEVRP
MEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMD
RDKVTKATAQIGFIKFVLIPMFETVTKLFPMVE
EIMLQPLWESRDRYEELKRIDDAMKELQKKTDS
LTSGATEKSRERSRDVKNSEGDCA*GKRGAWLQF
WTGWPSCAGSLCREELPWAPGTTRPCFLRTILF
TDTKKKKGIHDAVQNFIFKLSFK*YILIRK

Frame 2

PRRLASGKYSKKSECSRRAQDGIRLLQLPAQGH
LPGHRWTHSEGTRPSPC*PPTTPWSPSTPPCPR
IQNALRTK*DLWPSSNSPLVSRTREPQAVASLL
RDH*GTDGLWAWSSPGGKEHLKVDR*SPGPESPR
AATRKASASLQREKN*SRACWRRLQSSSQEHSK
SMN*KLKLQITWLS*RNAWNWKD*KWWRLRNAR
VTLRR*GRSWRPEAAGPTAPVSTVFWITTRS*LL
DAMFPLTPSTCSLQRPSRPCGSRPLTSGFGSPM
RC*AAWSTCTTTSGWSGTSASTLSPSGGGCSAS
TTTTETTPSTTSGTASAWPR*CTAWSGSAVSRRS
SHKRIS*S**QRPSATIWTIPATTTRTRSMPAQ
SWRSATMTSHRWRTTTAPWPSRSSPSLSATSSP
TSHLMGSSRSDRE*SH*SWPLTWQDMQKLWILSK
RKWRILTTATRST*PC*R*F**NAVISLTRSVQ
WKSQSLGWTVY*RNILCRATVRSQKAFLWHRSW
TETK*PRPQPRLGSSSLS*SQCLKQ*PSSSPWLR
RSCCSHFGNPEIATRS*SG*MTP*KSYRRRLTA
*RLGPPRSPEREAEM*KTVKETVPEESGGRGCS
SGRAGRAARDPCAGKSCPGHLAPQDHVF*EPFCS
LIQKKKKEFMMLYRILFLNCLLNNIFLYG

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