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Sequences

Sequence NM_021736

GTCCAGAGTGGCAGTAAAGGAGGAAGATGGCGGGGTGCAGGGGGTCTCTGTGCTGCTGCTGCAGGTGGTGCTGCTGCTGCGGTGAGCGTGAGACCCGCAC CCCCGAGGAGCTGACCATCCTTGGAGAAACACAGGAGGAGGAGGATGAGATTCTTCCAAGGAAAGACTATGAGAGTTTGGATTATGATCGCTGTATCAAT GACCCTTACCTGGAAGTTTTGGAGACCATGGATAATAAGAAAGGTCGAAGATATGAGGCGGTGAAGTGGATGGTGGTGTTTGCCATTGGAGTCTGCACTG GCCTGGTGGGTCTCTTTGTGGACTTTTTTGTGCGACTCTTCACCCAACTCAAGTTCGGAGTGGTACAGACATCGGTGGAGGAGTGCAGCCAGAAAGGCTG CCTCGCTCTGTCTCTCCTTGAACTCCTGGGTTTTAACCTCACCTTTGTCTTCCTGGCAAGCCTCCTTGTTCTCATTGAGCCGGTGGCAGCAGGTTCCGGG ATACCCGAGGTCAAATGCTATCTGAATGGCGTAAAGGTGCCAGGAATCGTCCGTCTCCGGACCCTGCTCTGCAAGGTCCTTGGAGTGCTGTTCAGTGTGG CTGGAGGGCTCTTCGTGGAGAAGGAAGGCCCCATGATCCACAGTGGTTCGGTGGTGGGAGCTGGCCTCCCTCAGTTTCAGAGCATCTCCTTACGGAAGAT CCAGTTTAACTTCCCCTATTTCCGAAGCGACAGAGACAAGAGAGACTTTGTATCAGCAGGAGCGGCTGCTGGAGTTGCTGCAGCTTTCGGGGCGCCAATC GGGGGTACCTTGTTCAGTCTAGAGGAGGGTTCGTCCTTCTGGAACCAAGGGCTCACGTGGAAAGTGCTCTTTTGTTCCATGTCTGCCACCTTCACCCTCA ACTTCTTCCGTTCTGGGATTCAGTTTGGAAGCTGGGGTTCCTTCCAGCTCCCTGGATTGCTGAACTTTGGCGAGTTTAAGAGTCTTAGAGAGCCTCCTTG TGTCTCTGGTAACCACCGTGGTGGTGTTTGTGGCCTCGATGGTGTTAGGAGAATGCCGACAGATGTCCTCTTCGAGTCAAATCGGTAACAGCCCAGCCCA CCCTCTCCTGGTGCTGCCTGGGGAGGCAAATCATGCTCACTCCGGCGGGCACAGCTGGCTGGGGCTGTTCCGGGGCATGGAAGATTCCCAGTCACCCACT CACTCAGAAAGCCGGGAGTCATCGGACACCTTGCTGGTCAGAGGTCCTGGGGGTGGTTTTGAACCATCAGAGCTTGGACTTTTCTGACTTCCCCAGCAAG GATCTTCCCACTTCCTGCTCCCTGTGTTCCCACCCTCCAGTGTTGGCACAGGCCCACCCCTGGCTCCACCAGAGCCAGAAGCAGAGGTAGAATCAGGCGG GCCCCGGGCTGCACTCCGAGCAGTGTTCCTGGCCATCTTTGCTACTTTCCTAGAGAACCCGGCTGTTGCCTTAAATGTGTGAGAGGGACTTGGCCAAGGC AAAAGCTGGGGAGATGCCAGTGACAACATACAGTTCATGACTAGGTTTAGGAATTGGGCACTGAGAAAATTCTCAATATTTCAGAGAGTCCTTCCCTTAT TTGGGACTCTTAACACGGTATCCTCGCTAGTTGGTTTTAAGGGAAACACTCTGCTCCTGGGTGTGAGCAGAGGCTCTGGTCTTGCCCTGTGGTTTGACTC TCCTTAGAACCACCGCCCACCAGAAACATAAAGGATTAAAATCACACTAATAACCCCTGGATGGTCAATCTGATAATAGGATCAGATTTACGTCTACCCT AATTCTTAACATTGCAGCTTTCTCTCCATCTGCAGATTATTCCCAGTCTCCCAGTAACACGTTTCTACCCAGATCCTTTTTCATTTCCTTAAGTTTTGAT CTCCGTCTTCCTGATGAAGCAGGCAGAGCTCAGAGGATCTTGGCATCACCCACCAAAGTTAGCTGAAAGCAGGGCACTCCTGGATAAAGCAGCTTCACTC AACTCTGGGGAATGCTACCATTTTTTTTCCAAAGTAGAAAGGAAGCACTTCTGAGCCAGTGACCACTGAAAGGTATGTGCTATGATAAAGCAGATGGCCT ATTTGAGGAAGAGGGTGTCTGCCCTTCACAAACACCTCTCTCTCCCCTGCACTAGCTGTCCCAAGCTTACATACAGAGGCCCTTCAGGAGGGCCTCCTGT GCCGCAGGGAGGGTGCGTGGGGAAGATGCTTCCTGCCAGCACGTGCCTGAAGGTTTCACATGAAGCATGGGAAGCGCACCCTGTCGTTCAGTGACGTCAT TCTTCTCCAGGCTGGCCCGCCCCCTCTGACTAGGCACCCAAAGTGAGCATCTGGGCATTGGGCATTCATGCTTATCTTCCCCCACCTTCTACATGGTATT AGTCCCAGCAGGCATCCCTGGGGCAGACGTGCTTTGGCTCAAGATGGCCTTCATTTACGTTTAGTTTTTTTTAAAACCGTGGAGGTTGCCCACGGGCCTC GGCACCTGGCCCTGGCAGCACAGCTCTCAGGCCCAGCCCTGGGCGACCTCCTTGGCCAAGTCTGCCTTTCACCCTGGGGTGAGCATCAGTCCTGGCTCTG CTGGTCCAGATCTTGCGCTCAGCACACTCTAGGGAATAATTCCACTCCAGAGATGGGGCTGCTTCAAGGTCTTTTCTAGCTGATTGTGGCCCCTCCATTT TCCCCATTTTCTTATCTCCCTGACCAAAATTGCTTTGACTTCTAAATGTTTCTGCTTCCCAGAATGCACCTGACTTATGAAATGGGGATAATACTCCCAG GAAATAGCGCAGGACATCACAAGGACCAAAAAGGCAATTCTTATTTAAATGTTACTATTTGGCCAGCTGCTGCTGTGTTTTATGGCAGTGTTCAGAGCTT GATCACGTTATTTCTTCCTTTTATTAAGAAGGAAGCCAATTGTCCAAGTCAGGAGAATGGTGTGATCACCTGTCACAGACACTTTGTCCCCTCTCCCCGC CCCTTCCTGGAGCTGGCAGAGCTAACGCCCTGCAGGAGGACCCCGGCCTCTCGAGGGCTGGATCAGCAGCCGCCTGCCCTGAGGCTGCCCCGGTGAATGT TATTGGAATTCATCCCTCGTGCACATCCTGTTGTGTTTAAGTCACCAGATATTTTGTTCCCATCAGTTTAGCCCAGAGATAGACAGTAGAATGCAAATAC CTCCCTCCCCTAAACTGACTGGACGGCTGCCAAGGAGGCCCCAAACCCAGGCCCCATGCAAAGGCACGTGGTTTCCTTTTCTCCTCTCTCTGCATCTGCG CTTTCCAGATAAGCCCAAAGACAGCAACTTCTCCACTCATGACAAATCAACTGTGACCCTCGCTCCTTCCATTTCTGTCCATTAGAAACCAGCCTTTTCA GCATCTCACCCATTAGCAGCCCCATCACCCAGTGATCAGTCGCCTCAGTAAAGCAGATCTGTGGATGGGGAGCCTACGGGTGGTAAGAAGTGGTGTTTTG TGTTTCATCTCCAGCTTGGTGTTCCATGGCCCCTAGGCGAGGTGATCAGGGAGTGGGGCCAATGGGCCCCCGGCCCTGGCTTTGGGACCTTGTGCTGAGG GATGATTTGCTCCTGACCTTGATTAACTTAACAGTTCCCAGCTGGAAGGGACACTTTCAGGACCCAGTCCACTGTATGGCATTTGTGATGCAGAATTATG CACTGACATGACCCTGGGTGACAGGAAAGCCTTTCGAGAGGCCCAAGGTGGCCTCGCCAGCCCTGCAGTATTGATGTGCAGTATTGCACCACAGCTCTGC GGACCTTGGCCATTGCCGCAGTCGCAGCTTCCTTTTTTCTGTTTGCACTGTTTGTTTGTATGATGTTAGCTAATTCCACTGTGTATATAAATTGTATTTT TTTTAATTTGTAAAATGCTATTTTTATTTGAACCTTTGGAACTTGGGAGTTCTCATTGTAACCCTAACATGTGAGAATAAAATGTCTTCTGTC

refseq_CLCN6.F1 (forward primer)
refseq_CLCN6.R1 (reverse primer)
Single exon skipping

Frame 0

VQSGSKGGRWRGAGGLCAAAAGGAAAAVSVRPA PPRS*PSLEKHRRRRMRFFQGKTMRVWIMIAVSM TLTWKFWRPWIIRKVEDMRR*SGWWCLPLESAL AWWVSLWTFLCDSSPNSSSEWYRHRWRSAARKA ASLCLSLNSWVLTSPLSSWQASLFSLSRWQQVPG YPRSNAI*MA*RCQESSVSGPCSARSLECCSVW LEGSSWRRKAP*STVVRWWELASLSFRASPYGR SSLTSPISEATETRETLYQQERLLELLQLSGRQS GVPCSV*RRVRPSGTKGSRGKCSFVPCLPPSPS TSSVLGFSLEAGVPSSSLDC*TLASLRVLESLL VSLVTTVVVFVASMVLGECRQMSSSSQIGNSPAH PLLVLPGEANHAHSGGHSWLGLFRGMEDSQSPT HSESRESSDTLLVRGPGGGFEPSELGLF*LPQQ GSSHFLLPVFPPSSVGTGPPLAPPEPEAEVESGG PRAALRAVFLAIFATFLENPAVALNV*EGLGQG KSWGDASDNIQFMTRFRNWALRKFSIFQRVLPL FGTLNTVSSLVGFKGNTLLLGVSRGSGLALWFDS P*NHRPPET*RIKITLITPGWSI***DQIYVYP NS*HCSFLSICRLFPVSQ*HVSTQILFHFLKF* SPSS**SRQSSEDLGITHQS*LKAGHSWIKQLHS TLGNATIFFPK*KGSTSEPVTTERYVL**SRWP I*GRGCLPFTNTSLSPALAVPSLHTEALQEGLL CRREGAWGRCFLPARA*RFHMKHGKRTLSFSDVI LLQAGPPPLTRHPK*ASGHWAFMLIFPHLLHGI SPSRHPWGRRALAQDGLHLRLVFFKTVEVAHGP RHLALAAQLSGPALGDLLGQVCLSPWGEHQSWLC WSRSCAQHTLGNNSTPEMGLLQGLF*LIVAPPF SPFSYLPDQNCFDF*MFLLPRMHLTYEMGIILP GNSAGHHKDQKGNSYLNVTIWPAAAVFYGSVQSL ITLFLPFIKKEANCPSQENGVITCHRHFVPSPR PFLELAELTPCRRTPASRGLDQQPPALRLPR*M LLEFIPRAHPVVFKSPDILFPSV*PRDRQ*NANT SLP*TDWTAAKEAPNPGPMQRHVVSFSPLSASA LSR*AQRQQLLHS*QINCDPRSFHFCPLETSLF SISPISSPITQ*SVASVKQICGWGAYGW*EVVFC VSSPAWCSMAPRRGDQGVGPMGPRPWLWDLVLR DDLLLTLINLTVPSWKGHFQDPVHCMAFVMQNY ALT*PWVTGKPFERPKVASPALQY*CAVLHHSSA DLGHCRSRSFLFSVCTVCLYDVS*FHCVYKLYF F*FVKCYFYLNLWNLGVLIVTLTCENKMSSV

Frame 1

SRVAVKEEDGGVQGVSVLLLQVVLLLR*A*DPH PRGADHPWRNTGGGG*DSSKERL*EFGL*SLYQ *PLPGSFGDHG**ERSKI*GGEVDGGVCHWSLHW PGGSLCGLFCATLHPTQVRSGTDIGGGVQPERL PRSVSP*TPGF*PHLCLPGKPPCSH*AGGSRFR DTRGQMLSEWRKGARNRPSPDPALQGPWSAVQCG WRALRGEGRPHDPQWFGGGSWPPSVSEHLLTED PV*LPLFPKRQRQERLCISRSGCWSCCSFRGAN RGYLVQSRGGFVLLEPRAHVESALLFHVCHLHPQ LLPFWDSVWKLGFLPAPWIAELWRV*ES*RASL CLW*PPWWCLWPRWC*ENADRCPLRVKSVTAQP TLSWCCLGRQIMLTPAGTAGWGCSGAWKIPSHPL TQKAGSHRTPCWSEVLGVVLNHQSLDFSDFPSK DLPTSCSLCSHPPVLAQAHPWLHQSQKQR*NQA GPGLHSEQCSWPSLLLS*RTRLLP*MCERDLAKA KAGEMPVTTYSS*LGLGIGH*ENSQYFRESFPY LGLLTRYPR*LVLRETLCSWV*AEALVLPCGLT LLRTTAHQKHKGLKSH**PLDGQSDNRIRFTSTL ILNIAAFSPSADYSQSPSNTFLPRSFFISLSFD LRLPDEAGRAQRILASPTKVS*KQGTPG*SSFT QLWGMLPFFFQSRKEALLSQ*PLKGMCYDKADGL FEEEGVCPSQTPLSPLH*LSQAYIQRPFRRASC AAGRVRGEDASCQHVPEGFT*SMGSAPCRSVTS FFSRLARPL*LGTQSEHLGIGHSCLSSPTFYMVL VPAGIPGADVLWLKMAFIYV*FFLKPWRLPTGL GTWPWQHSSQAQPWATSLAKSAFHPGVSISPGS AGPDLALSTL*GIIPLQRWGCFKVFSS*LWPLHF PHFLISLTKIALTSKCFCFPECT*LMKWG*YSQ EIAQDITRTKKAILI*MLLFGQLLLCFMAVFRA *SRYFFLLLRRKPIVQVRRMV*SPVTDTLSPLPA PSWSWQS*RPAGGPRPLEGWISSRLP*GCPGEC YWNSSLVHILLCLSHQIFCSHQFSPEIDSRMQI PPSPKLTGRLPRRPQTQAPCKGTWFPFLLSLHLR FPDKPKDSNFSTHDKSTVTLAPSISVH*KPAFS ASHPLAAPSPSDQSPQ*SRSVDGEPTGGKKWCF VFHLQLGVPWPLGEVIREWGQWAPGPGFGTLC*G MICS*P*LT*QFPAGRDTFRTQSTVWHL*CRIM H*HDPG*QESLSRGPRWPRQPCSIDVQYCTTAL RTLAIAAVAASFFLFALFVCMMLANSTVYINCIF FNL*NAIFI*TFGTWEFSL*P*HVRIKCLL

Frame 2

PEWQ*RRKMAGCRGSLCCCCRWCCCCGERETRT PEELTILGETQEEEDEILPRKDYESLDYDRCIN DPYLEVLETMDNKKGRRYEAVKWMVVFAIGVCT GLVGLFVDFFVRLFTQLKFGVVQTSVEECSQKGC LALSLLELLGFNLTFVFLASLLVLIEPVAAGSG IPEVKCYLNGVKVPGIVRLRTLLCKVLGVLFSV AGGLFVEKEGPMIHSGSVVGAGLPQFQSISLRKI QFNFPYFRSDRDKRDFVSAGAAAGVAAAFGAPI GGTLFSLEEGSSFWNQGLTWKVLFCSMSATFTL NFFRSGIQFGSWGSFQLPGLLNFGEFKSLREPPC VSGNHRGGVCGLDGVRRMPTDVLFESNR*QPSP PSPGAAWGGKSCSLRRAQLAGAVPGHGRFPVTH SLRKPGVIGHLAGQRSWGWF*TIRAWTFLTSPAR IFPLPAPCVPTLQCWHRPTPGSTRARSRGRIRR APGCTPSSVPGHLCYFPREPGCCLKCVRGTWPR QKLGRCQ*QHTVHD*V*ELGTEKILNISESPSLI WDS*HGILASWF*GKHSAPGCEQRLWSCPVV*L SLEPPPTRNIKD*NHTNNPWMVNLIIGSDLRLP *FLTLQLSLHLQIIPSLPVTRFYPDPFSFP*VLI SVFLMKQAELRGSWHHPPKLAESRALLDKAASL NSGECYHFFSKVERKHF*ASDH*KVCAMIKQMA YLRKRVSALHKHLSLPCTSCPKLTYRGPSGGPPV PQGGCVGKMLPASTCLKVSHEAWEAHPVVQ*RH SSPGWPAPSD*APKVSIWALGIHAYLPPPSTWY *SQQASLGQTCFGSRWPSFTFSFF*NRGGCPRAS APGPGSTALRPSPGRPPWPSLPFTLG*ASVLAL LVQILRSAHSRE*FHSRDGAASRSFLADCGPSI FPIFLSP*PKLL*LLNVSASQNAPDL*NGDNTPR K*RRTSQGPKRQFLFKCYYLASCCCVLWQCSEL DHVISSFY*EGSQLSKSGEWCDHLSQTLCPLSP PLPGAGRANALQEDPGLSRAGSAAACPEAAPVNV IGIHPSCTSCCV*VTRYFVPISLAQR*TVECKY LPPLN*LDGCQGGPKPRPHAKARGFLFSSLCIC AFQISPKTATSPLMTNQL*PSLLPFLSIRNQPFQ HLTH*QPHHPVISRLSKADLWMGSLRVVRSGVL CFISSLVFHGP*AR*SGSGANGPPALALGPCAE G*FAPDLD*LNSSQLEGTLSGPSPLYGICDAELC TDMTLGDRKAFREAQGGLASPAVLMCSIAPQLC GPWPLPQSQLPFFCLHCLFV*C*LIPLCI*IVF FLICKMLFLFEPLELGSSHCNPNM*E*NVFC

Sequence NM_001286

GTCCAGAGTGGCAGTAAAGGAGGAAGATGGCGGGGTGCAGGGGGTCTCTGTGCTGCTGCTGCAGGTGGTGCTGCTGCTGCGGTGAGCGTGAGACCCGCAC CCCCGAGGAGCTGACCATCCTTGGAGAAACACAGGAGGAGGAGGATGAGATTCTTCCAAGGAAAGACTATGAGAGTTTGGATTATGATCGCTGTATCAAT GACCCTTACCTGGAAGTTTTGGAGACCATGGATAATAAGAAAGGTCGAAGATATGAGGCGGTGAAGTGGATGGTGGTGTTTGCCATTGGAGTCTGCACTG GCCTGGTGGGTCTCTTTGTGGACTTTTTTGTGCGACTCTTCACCCAACTCAAGTTCGGAGTGGTACAGACATCGGTGGAGGAGTGCAGCCAGAAAGGCTG CCTCGCTCTGTCTCTCCTTGAACTCCTGGGTTTTAACCTCACCTTTGTCTTCCTGGCAAGCCTCCTTGTTCTCATTGAGCCGGTGGCAGCAGGTTCCGGG ATACCCGAGGTCAAATGCTATCTGAATGGCGTAAAGGTGCCAGGAATCGTCCGTCTCCGGACCCTGCTCTGCAAGGTCCTTGGAGTGCTGTTCAGTGTGG CTGGAGGGCTCTTCGTGGAGAAGGAAGGCCCCATGATCCACAGTGGTTCGGTGGTGGGAGCTGGCCTCCCTCAGTTTCAGAGCATCTCCTTACGGAAGAT CCAGTTTAACTTCCCCTATTTCCGAAGCGACAGAGACAAGAGAGACTTTGTATCAGCAGGAGCGGCTGCTGGAGTTGCTGCAGCTTTCGGGGCGCCAATC GGGGGTACCTTGTTCAGTCTAGAGGAGGGTTCGTCCTTCTGGAACCAAGGGCTCACGTGGAAAGTGCTCTTTTGTTCCATGTCTGCCACCTTCACCCTCA ACTTCTTCCGTTCTGGGATTCAGTTTGGAAGCTGGGGTTCCTTCCAGCTCCCTGGATTGCTGAACTTTGGCGAGTTTAAGTGCTCTGACTCTGATAAAAA ATGTCATCTCTGGACAGCTATGGATTTGGGTTTCTTCGTCGTGATGGGGGTCATTGGGGGCCTCCTGGGAGCCACATTCAACTGTCTGAACAAGAGGCTT GCAAAGTACCGTATGCGAAACGTGCACCCGAAACCTAAGCTCGTCAGAGTCTTAGAGAGCCTCCTTGTGTCTCTGGTAACCACCGTGGTGGTGTTTGTGG CCTCGATGGTGTTAGGAGAATGCCGACAGATGTCCTCTTCGAGTCAAATCGGTAATGACTCATTCCAGCTCCAGGTCACAGAAGATGTGAATTCAAGTAT CAAGACATTTTTTTGTCCCAATGATACCTACAATGACATGGCCACACTCTTCTTCAACCCGCAGGAGTCTGCCATCCTCCAGCTCTTCCACCAGGATGGT ACTTTCAGCCCCGTCACTCTGGCCTTGTTCTTCGTTCTCTATTTCTTGCTTGCATGTTGGACTTACGGCATTTCTGTTCCAAGTGGCCTTTTTGTGCCTT CTCTGCTGTGTGGAGCTGCTTTTGGACGTTTAGTTGCCAATGTCCTAAAAAGCTACATTGGATTGGGCCACATCTATTCGGGGACCTTTGCCCTGATTGG TGCAGCGGCTTTCTTGGGCGGGGTGGTCCGCATGACCATCAGCCTCACGGTCATCCTGATCGAGTCCACCAATGAGATCACCTACGGGCTCCCCATCATG GTCACACTGATGGTGGCCAAATGGACAGGGGACTTTTTCAATAAGGGCATTTATGATATCCACGTGGGCCTGCGAGGCGTGCCGCTTCTGGAATGGGAGA CAGAGGTGGAAATGGACAAGCTGAGAGCCAGCGACATCATGGAGCCCAACCTGACCTACGTCTACCCGCACACCCGCATCCAGTCTCTGGTGAGCATCCT GCGCACCACGGTCCACCATGCCTTCCCGGTGGTCACAGAGAACCGCGGTAACGAGAAGGAGTTCATGAAGGGCAACCAGCTCATCAGCAACAACATCAAG TTCAAGAAATCCAGCATCCTCACCCGGGCTGGCGAGCAGCGCAAACGGAGCCAGTCCATGAAGTCCTACCCATCCAGCGAGCTACGGAACATGTGTGATG AGCACATCGCCTCTGAGGAGCCAGCCGAGAAGGAGGACCTCCTGCAGCAGATGCTGGAAAGGAGATACACTCCCTACCCCAACCTATACCCTGACCAGTC CCCAAGTGAAGACTGGACCATGGAGGAGCGGTTCCGCCCTCTGACCTTCCACGGCCTGATCCTTCGGTCGCAGCTTGTCACCCTGCTTGTCCGAGGAGTT TGTTACTCTGAAAGCCAGTCGAGCGCCAGCCAGCCGCGCCTCTCCTATGCCGAGATGGCCGAGGACTACCCGCGGTACCCCGACATCCACGACCTGGACC TGACGCTGCTCAACCCGCGCATGATCGTGGATGTCACCCCATACATGAACCCTTCGCCTTTCACCGTCTCGCCCAACACCCACGTCTCCCAAGTCTTCAA CCTGTTCAGAACGATGGGCCTGCGCCACCTGCCCGTGGTGAACGCTGTGGGAGAGATCGTGGGGATCATCACACGGCACAACCTCACCTATGAATTTCTG CAGGCCCGGCTGAGGCAGCACTACCAGACCATCTGACAGCCCAGCCCACCCTCTCCTGGTGCTGCCTGGGGAGGCAAATCATGCTCACTCCGGCGGGCAC AGCTGGCTGGGGCTGTTCCGGGGCATGGAAGATTCCCAGTCACCCACTCACTCAGAAAGCCGGGAGTCATCGGACACCTTGCTGGTCAGAGGTCCTGGGG GTGGTTTTGAACCATCAGAGCTTGGACTTTTCTGACTTCCCCAGCAAGGATCTTCCCACTTCCTGCTCCCTGTGTTCCCACCCTCCAGTGTTGGCACAGG CCCACCCCTGGCTCCACCAGAGCCAGAAGCAGAGGTAGAATCAGGCGGGCCCCGGGCTGCACTCCGAGCAGTGTTCCTGGCCATCTTTGCTACTTTCCTA GAGAACCCGGCTGTTGCCTTAAATGTGTGAGAGGGACTTGGCCAAGGCAAAAGCTGGGGAGATGCCAGTGACAACATACAGTTCATGACTAGGTTTAGGA ATTGGGCACTGAGAAAATTCTCAATATTTCAGAGAGTCCTTCCCTTATTTGGGACTCTTAACACGGTATCCTCGCTAGTTGGTTTTAAGGGAAACACTCT GCTCCTGGGTGTGAGCAGAGGCTCTGGTCTTGCCCTGTGGTTTGACTCTCCTTAGAACCACCGCCCACCAGAAACATAAAGGATTAAAATCACACTAATA ACCCCTGGATGGTCAATCTGATAATAGGATCAGATTTACGTCTACCCTAATTCTTAACATTGCAGCTTTCTCTCCATCTGCAGATTATTCCCAGTCTCCC AGTAACACGTTTCTACCCAGATCCTTTTTCATTTCCTTAAGTTTTGATCTCCGTCTTCCTGATGAAGCAGGCAGAGCTCAGAGGATCTTGGCATCACCCA CCAAAGTTAGCTGAAAGCAGGGCACTCCTGGATAAAGCAGCTTCACTCAACTCTGGGGAATGCTACCATTTTTTTTCCAAAGTAGAAAGGAAGCACTTCT GAGCCAGTGACCACTGAAAGGTATGTGCTATGATAAAGCAGATGGCCTATTTGAGGAAGAGGGTGTCTGCCCTTCACAAACACCTCTCTCTCCCCTGCAC TAGCTGTCCCAAGCTTACATACAGAGGCCCTTCAGGAGGGCCTCCTGTGCCGCAGGGAGGGTGCGTGGGGAAGATGCTTCCTGCCAGCACGTGCCTGAAG GTTTCACATGAAGCATGGGAAGCGCACCCTGTCGTTCAGTGACGTCATTCTTCTCCAGGCTGGCCCGCCCCCTCTGACTAGGCACCCAAAGTGAGCATCT GGGCATTGGGCATTCATGCTTATCTTCCCCCACCTTCTACATGGTATTAGTCCCAGCAGGCATCCCTGGGGCAGACGTGCTTTGGCTCAAGATGGCCTTC ATTTACGTTTAGTTTTTTTTAAAACCGTGGAGGTTGCCCACGGGCCTCGGCACCTGGCCCTGGCAGCACAGCTCTCAGGCCCAGCCCTGGGCGACCTCCT TGGCCAAGTCTGCCTTTCACCCTGGGGTGAGCATCAGTCCTGGCTCTGCTGGTCCAGATCTTGCGCTCAGCACACTCTAGGGAATAATTCCACTCCAGAG ATGGGGCTGCTTCAAGGTCTTTTCTAGCTGATTGTGGCCCCTCCATTTTCCCCATTTTCTTATCTCCCTGACCAAAATTGCTTTGACTTCTAAATGTTTC TGCTTCCCAGAATGCACCTGACTTATGAAATGGGGATAATACTCCCAGGAAATAGCGCAGGACATCACAAGGACCAAAAAGGCAATTCTTATTTAAATGT TACTATTTGGCCAGCTGCTGCTGTGTTTTATGGCAGTGTTCAGAGCTTGATCACGTTATTTCTTCCTTTTATTAAGAAGGAAGCCAATTGTCCAAGTCAG GAGAATGGTGTGATCACCTGTCACAGACACTTTGTCCCCTCTCCCCGCCCCTTCCTGGAGCTGGCAGAGCTAACGCCCTGCAGGAGGACCCCGGCCTCTC GAGGGCTGGATCAGCAGCCGCCTGCCCTGAGGCTGCCCCGGTGAATGTTATTGGAATTCATCCCTCGTGCACATCCTGTTGTGTTTAAGTCACCAGATAT TTTGTTCCCATCAGTTTAGCCCAGAGATAGACAGTAGAATGCAAATACCTCCCTCCCCTAAACTGACTGGACGGCTGCCAAGGAGGCCCCAAACCCAGGC CCCATGCAAAGGCACGTGGTTTCCTTTTCTCCTCTCTCTGCATCTGCGCTTTCCAGATAAGCCCAAAGACAGCAACTTCTCCACTCATGACAAATCAACT GTGACCCTCGCTCCTTCCATTTCTGTCCATTAGAAACCAGCCTTTTCAGCATCTCACCCATTAGCAGCCCCATCACCCAGTGATCAGTCGCCTCAGTAAA GCAGATCTGTGGATGGGGAGCCTACGGGTGGTAAGAAGTGGTGTTTTGTGTTTCATCTCCAGCTTGGTGTTCCATGGCCCCTAGGCGAGGTGATCAGGGA GTGGGGCCAATGGGCCCCCGGCCCTGGCTTTGGGACCTTGTGCTGAGGGATGATTTGCTCCTGACCTTGATTAACTTAACAGTTCCCAGCTGGAAGGGAC ACTTTCAGGACCCAGTCCACTGTATGGCATTTGTGATGCAGAATTATGCACTGACATGACCCTGGGTGACAGGAAAGCCTTTCGAGAGGCCCAAGGTGGC CTCGCCAGCCCTGCAGTATTGATGTGCAGTATTGCACCACAGCTCTGCGGACCTTGGCCATTGCCGCAGTCGCAGCTTCCTTTTTTCTGTTTGCACTGTT TGTTTGTATGATGTTAGCTAATTCCACTGTGTATATAAATTGTATTTTTTTTAATTTGTAAAATGCTATTTTTATTTGAACCTTTGGAACTTGGGAGTTC TCATTGTAACCCTAACATGTGAGAATAAAATGTCTTCTGTC

refseq_CLCN6.F1 (forward primer)
refseq_CLCN6.R1 (reverse primer)
Single exon skipping

Frame 0

VQSGSKGGRWRGAGGLCAAAAGGAAAAVSVRPA PPRS*PSLEKHRRRRMRFFQGKTMRVWIMIAVSM TLTWKFWRPWIIRKVEDMRR*SGWWCLPLESAL AWWVSLWTFLCDSSPNSSSEWYRHRWRSAARKA ASLCLSLNSWVLTSPLSSWQASLFSLSRWQQVPG YPRSNAI*MA*RCQESSVSGPCSARSLECCSVW LEGSSWRRKAP*STVVRWWELASLSFRASPYGR SSLTSPISEATETRETLYQQERLLELLQLSGRQS GVPCSV*RRVRPSGTKGSRGKCSFVPCLPPSPS TSSVLGFSLEAGVPSSSLDC*TLASLSALTLIK NVISGQLWIWVSSS*WGSLGASWEPHSTV*TRGL QSTVCETCTRNLSSSES*RASLCLW*PPWWCLW PRWC*ENADRCPLRVKSVMTHSSSRSQKM*IQV SRHFFVPMIPTMTWPHSSSTRRSLPSSSSSTRMV LSAPSLWPCSSFSISCLHVGLTAFLFQVAFLCL LCCVELLLDV*LPMS*KATLDWATSIRGPLP*L VQRLSWAGWSA*PSASRSS*SSPPMRSPTGSPSW SH*WWPNGQGTFSIRAFMISTWACEACRFWNGR QRWKWTS*EPATSWSPT*PTSTRTPASSLW*AS CAPRSTMPSRWSQRTAVTRRSS*RATSSSATTSS SRNPASSPGLASSANGASP*SPTHPASYGTCVM STSPLRSQPRRRTSCSRCWKGDTLPTPTYTLTS PQVKTGPWRSGSAL*PSTA*SFGRSLSPCLSEEF VTLKASRAPASRASPMPRWPRTTRGTPTSTTWT *RCSTRA*SWMSPHT*TLRLSPSRPTPTSPKSS TCSERWACATCPW*TLWERSWGSSHGTTSPMNFC RPG*GSTTRPSDSPAHPLLVLPGEANHAHSGGH SWLGLFRGMEDSQSPTHSESRESSDTLLVRGPG GGFEPSELGLF*LPQQGSSHFLLPVFPPSSVGTG PPLAPPEPEAEVESGGPRAALRAVFLAIFATFL ENPAVALNV*EGLGQGKSWGDASDNIQFMTRFR NWALRKFSIFQRVLPLFGTLNTVSSLVGFKGNTL LLGVSRGSGLALWFDSP*NHRPPET*RIKITLI TPGWSI***DQIYVYPNS*HCSFLSICRLFPVS Q*HVSTQILFHFLKF*SPSS**SRQSSEDLGITH QS*LKAGHSWIKQLHSTLGNATIFFPK*KGSTS EPVTTERYVL**SRWPI*GRGCLPFTNTSLSPA LAVPSLHTEALQEGLLCRREGAWGRCFLPARA*R FHMKHGKRTLSFSDVILLQAGPPPLTRHPK*AS GHWAFMLIFPHLLHGISPSRHPWGRRALAQDGL HLRLVFFKTVEVAHGPRHLALAAQLSGPALGDLL GQVCLSPWGEHQSWLCWSRSCAQHTLGNNSTPE MGLLQGLF*LIVAPPFSPFSYLPDQNCFDF*MF LLPRMHLTYEMGIILPGNSAGHHKDQKGNSYLNV TIWPAAAVFYGSVQSLITLFLPFIKKEANCPSQ ENGVITCHRHFVPSPRPFLELAELTPCRRTPAS RGLDQQPPALRLPR*MLLEFIPRAHPVVFKSPDI LFPSV*PRDRQ*NANTSLP*TDWTAAKEAPNPG PMQRHVVSFSPLSASALSR*AQRQQLLHS*QIN CDPRSFHFCPLETSLFSISPISSPITQ*SVASVK QICGWGAYGW*EVVFCVSSPAWCSMAPRRGDQG VGPMGPRPWLWDLVLRDDLLLTLINLTVPSWKG HFQDPVHCMAFVMQNYALT*PWVTGKPFERPKVA SPALQY*CAVLHHSSADLGHCRSRSFLFSVCTV CLYDVS*FHCVYKLYFF*FVKCYFYLNLWNLGV LIVTLTCENKMSSV

Frame 1

SRVAVKEEDGGVQGVSVLLLQVVLLLR*A*DPH PRGADHPWRNTGGGG*DSSKERL*EFGL*SLYQ *PLPGSFGDHG**ERSKI*GGEVDGGVCHWSLHW PGGSLCGLFCATLHPTQVRSGTDIGGGVQPERL PRSVSP*TPGF*PHLCLPGKPPCSH*AGGSRFR DTRGQMLSEWRKGARNRPSPDPALQGPWSAVQCG WRALRGEGRPHDPQWFGGGSWPPSVSEHLLTED PV*LPLFPKRQRQERLCISRSGCWSCCSFRGAN RGYLVQSRGGFVLLEPRAHVESALLFHVCHLHPQ LLPFWDSVWKLGFLPAPWIAELWRV*VL*L**K MSSLDSYGFGFLRRDGGHWGPPGSHIQLSEQEA CKVPYAKRAPET*ARQSLREPPCVSGNHRGGVCG LDGVRRMPTDVLFESNR**LIPAPGHRRCEFKY QDIFLSQ*YLQ*HGHTLLQPAGVCHPPALPPGW YFQPRHSGLVLRSLFLACMLDLRHFCSKWPFCAF SAVWSCFWTFSCQCPKKLHWIGPHLFGDLCPDW CSGFLGRGGPHDHQPHGHPDRVHQ*DHLRAPHH GHTDGGQMDRGLFQ*GHL*YPRGPARRAASGMGD RGGNGQAESQRHHGAQPDLRLPAHPHPVSGEHP AHHGPPCLPGGHREPR*REGVHEGQPAHQQQHQ VQEIQHPHPGWRAAQTEPVHEVLPIQRATEHV** AHRL*GASREGGPPAADAGKEIHSLPQPIP*PV PK*RLDHGGAVPPSDLPRPDPSVAACHPACPRS LLL*KPVERQPAAPLLCRDGRGLPAVPRHPRPGP DAAQPAHDRGCHPIHEPFAFHRLAQHPRLPSLQ PVQNDGPAPPARGERCGRDRGDHHTAQPHL*IS AGPAEAALPDHLTAQPTLSWCCLGRQIMLTPAGT AGWGCSGAWKIPSHPLTQKAGSHRTPCWSEVLG VVLNHQSLDFSDFPSKDLPTSCSLCSHPPVLAQ AHPWLHQSQKQR*NQAGPGLHSEQCSWPSLLLS* RTRLLP*MCERDLAKAKAGEMPVTTYSS*LGLG IGH*ENSQYFRESFPYLGLLTRYPR*LVLRETL CSWV*AEALVLPCGLTLLRTTAHQKHKGLKSH** PLDGQSDNRIRFTSTLILNIAAFSPSADYSQSP SNTFLPRSFFISLSFDLRLPDEAGRAQRILASP TKVS*KQGTPG*SSFTQLWGMLPFFFQSRKEALL SQ*PLKGMCYDKADGLFEEEGVCPSQTPLSPLH *LSQAYIQRPFRRASCAAGRVRGEDASCQHVPE GFT*SMGSAPCRSVTSFFSRLARPL*LGTQSEHL GIGHSCLSSPTFYMVLVPAGIPGADVLWLKMAF IYV*FFLKPWRLPTGLGTWPWQHSSQAQPWATS LAKSAFHPGVSISPGSAGPDLALSTL*GIIPLQR WGCFKVFSS*LWPLHFPHFLISLTKIALTSKCF CFPECT*LMKWG*YSQEIAQDITRTKKAILI*M LLFGQLLLCFMAVFRA*SRYFFLLLRRKPIVQVR RMV*SPVTDTLSPLPAPSWSWQS*RPAGGPRPL EGWISSRLP*GCPGECYWNSSLVHILLCLSHQI FCSHQFSPEIDSRMQIPPSPKLTGRLPRRPQTQA PCKGTWFPFLLSLHLRFPDKPKDSNFSTHDKST VTLAPSISVH*KPAFSASHPLAAPSPSDQSPQ* SRSVDGEPTGGKKWCFVFHLQLGVPWPLGEVIRE WGQWAPGPGFGTLC*GMICS*P*LT*QFPAGRD TFRTQSTVWHL*CRIMH*HDPG*QESLSRGPRW PRQPCSIDVQYCTTALRTLAIAAVAASFFLFALF VCMMLANSTVYINCIFFNL*NAIFI*TFGTWEF SL*P*HVRIKCLL

Frame 2

PEWQ*RRKMAGCRGSLCCCCRWCCCCGERETRT PEELTILGETQEEEDEILPRKDYESLDYDRCIN DPYLEVLETMDNKKGRRYEAVKWMVVFAIGVCT GLVGLFVDFFVRLFTQLKFGVVQTSVEECSQKGC LALSLLELLGFNLTFVFLASLLVLIEPVAAGSG IPEVKCYLNGVKVPGIVRLRTLLCKVLGVLFSV AGGLFVEKEGPMIHSGSVVGAGLPQFQSISLRKI QFNFPYFRSDRDKRDFVSAGAAAGVAAAFGAPI GGTLFSLEEGSSFWNQGLTWKVLFCSMSATFTL NFFRSGIQFGSWGSFQLPGLLNFGEFKCSDSDKK CHLWTAMDLGFFVVMGVIGGLLGATFNCLNKRL AKYRMRNVHPKPKLVRVLESLLVSLVTTVVVFV ASMVLGECRQMSSSSQIGNDSFQLQVTEDVNSSI KTFFCPNDTYNDMATLFFNPQESAILQLFHQDG TFSPVTLALFFVLYFLLACWTYGISVPSGLFVP SLLCGAAFGRLVANVLKSYIGLGHIYSGTFALIG AAAFLGGVVRMTISLTVILIESTNEITYGLPIM VTLMVAKWTGDFFNKGIYDIHVGLRGVPLLEWE TEVEMDKLRASDIMEPNLTYVYPHTRIQSLVSIL RTTVHHAFPVVTENRGNEKEFMKGNQLISNNIK FKKSSILTRAGEQRKRSQSMKSYPSSELRNMCD EHIASEEPAEKEDLLQQMLERRYTPYPNLYPDQS PSEDWTMEERFRPLTFHGLILRSQLVTLLVRGV CYSESQSSASQPRLSYAEMAEDYPRYPDIHDLD LTLLNPRMIVDVTPYMNPSPFTVSPNTHVSQVFN LFRTMGLRHLPVVNAVGEIVGIITRHNLTYEFL QARLRQHYQTI*QPSPPSPGAAWGGKSCSLRRA QLAGAVPGHGRFPVTHSLRKPGVIGHLAGQRSWG WF*TIRAWTFLTSPARIFPLPAPCVPTLQCWHR PTPGSTRARSRGRIRRAPGCTPSSVPGHLCYFP REPGCCLKCVRGTWPRQKLGRCQ*QHTVHD*V*E LGTEKILNISESPSLIWDS*HGILASWF*GKHS APGCEQRLWSCPVV*LSLEPPPTRNIKD*NHTN NPWMVNLIIGSDLRLP*FLTLQLSLHLQIIPSLP VTRFYPDPFSFP*VLISVFLMKQAELRGSWHHP PKLAESRALLDKAASLNSGECYHFFSKVERKHF *ASDH*KVCAMIKQMAYLRKRVSALHKHLSLPCT SCPKLTYRGPSGGPPVPQGGCVGKMLPASTCLK VSHEAWEAHPVVQ*RHSSPGWPAPSD*APKVSI WALGIHAYLPPPSTWY*SQQASLGQTCFGSRWPS FTFSFF*NRGGCPRASAPGPGSTALRPSPGRPP WPSLPFTLG*ASVLALLVQILRSAHSRE*FHSR DGAASRSFLADCGPSIFPIFLSP*PKLL*LLNVS ASQNAPDL*NGDNTPRK*RRTSQGPKRQFLFKC YYLASCCCVLWQCSELDHVISSFY*EGSQLSKS GEWCDHLSQTLCPLSPPLPGAGRANALQEDPGLS RAGSAAACPEAAPVNVIGIHPSCTSCCV*VTRY FVPISLAQR*TVECKYLPPLN*LDGCQGGPKPR PHAKARGFLFSSLCICAFQISPKTATSPLMTNQL *PSLLPFLSIRNQPFQHLTH*QPHHPVISRLSK ADLWMGSLRVVRSGVLCFISSLVFHGP*AR*SG SGANGPPALALGPCAEG*FAPDLD*LNSSQLEGT LSGPSPLYGICDAELCTDMTLGDRKAFREAQGG LASPAVLMCSIAPQLCGPWPLPQSQLPFFCLHC LFV*C*LIPLCI*IVFFLICKMLFLFEPLELGSS HCNPNM*E*NVFC