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Sequence NM_016725
TGGAGGCCTGGCTGGTGCTCACATACAATAATTAACTGCTGAGTGGCCTTCGCCCAATCCCAGGCTCCACTCCTGGGCTCCATTCCCACTCCCTGCCTGTCTCCTAGGCCACTAAACCACAGCTGTCCCCTGGAATAAGGCAAGGGGGAGTGTAGAGCAGAGCAGAAGCCTGAGCCAGACGGAGAGCCACCTCCTCTCCC
AGGGACAGACATGGCTCAGCGGATGACAACACAGCTGCTGCTCCTTCTAGTGTGGGTGGCTGTAGTAGGGGAGGCTCAGACAAGGATTGCATGGGCCAGG
ACTGAGCTTCTCAATGTCTGCATGAACGCCAAGCACCACAAGGAAAAGCCAGGCCCCGAGGACAAGTTGCATGAGCAGTGTCGACCCTGGAGGAAGAATG
CCTGCTGTTCTACCAACACCAGCCAGGAAGCCCATAAGGATGTTTCCTACCTATATAGATTCAACTGGAACCACTGTGGAGAGATGGCACCTGCCTGCAA
ACGGCATTTCATCCAGGACACCTGCCTCTACGAGTGCTCCCCCAACTTGGGGCCCTGGATCCAGCAGGTGGATCAGAGCTGGCGCAAAGAGCGGGTACTG
AACGTGCCCCTGTGCAAAGAGGACTGTGAGCAATGGTGGGAAGATTGTCGCACCTCCTACACCTGCAAGAGCAACTGGCACAAGGGCTGGAACTGGACTT
CAGGGTTTAACAAGTGCGCAGTGGGAGCTGCCTGCCAACCTTTCCATTTCTACTTCCCCACACCCACTGTTCTGTGCAATGAAATCTGGACTCACTCCTA
CAAGGTCAGCAACTACAGCCGAGGGAGTGGCCGCTGCATCCAGATGTGGTTCGACCCAGCCCAGGGCAACCCCAATGAGGAGGTGGCGAGGTTCTATGCT
GCAGCCATGAGTGGGGCTGGGCCCTGGGCAGCCTGGCCTTTCCTGCTTAGCCTGGCCCTAATGCTGCTGTGGCTGCTCAGCTGACCTCCTTTTACCTTCT
GATACCTGGAAATCCCTGCCCTGTTCAGCCCCACAGCTCCCAACTATTTGGTTCCTGCTCCATGGTCGGGCCTCTGACAGCCACTTTGAATAAACCAGAC
ACCGCACATGTGTCTTGAGAATTATTTGG
- refseq_FOLR1.F1 (forward primer)
- refseq_FOLR1.R1 (reverse primer)
- Single exon skipping
Frame 0
WRPGWCSHTIINC*VAFAQSQAPLLGSIPTPCLSPRPLNHSCPLE*GKGECRAEQKPEPDGEPPPLP
GTDMAQRMTTQLLLLLVWVAVVGEAQTRIAWAR
TELLNVCMNAKHHKEKPGPEDKLHEQCRPWRKN
ACCSTNTSQEAHKDVSYLYRFNWNHCGEMAPACK
RHFIQDTCLYECSPNLGPWIQQVDQSWRKERVL
NVPLCKEDCEQWWEDCRTSYTCKSNWHKGWNWT
SGFNKCAVGAACQPFHFYFPTPTVLCNEIWTHSY
KVSNYSRGSGRCIQMWFDPAQGNPNEEVARFYA
AAMSGAGPWAAWPFLLSLALMLLWLLS*PPFTF
*YLEIPALFSPTAPNYLVPAPWSGL*QPL*INQT
PHMCLENYL
Frame 1
GGLAGAHIQ*LTAEWPSPNPRLHSWAPFPLPACLLGH*TTAVPWNKARGSVEQSRSLSQTESHLLS
QGQTWLSG*QHSCCSF*CGWL**GRLRQGLHGPG
LSFSMSA*TPSTTRKSQAPRTSCMSSVDPGGRM
PAVLPTPARKPIRMFPTYIDSTGTTVERWHLPA
NGISSRTPASTSAPPTWGPGSSRWIRAGAKSGY*
TCPCAKRTVSNGGKIVAPPTPARATGTRAGTGL
QGLTSAQWELPANLSISTSPHPLFCAMKSGLTP
TRSATTAEGVAAASRCGSTQPRATPMRRWRGSML
QP*VGLGPGQPGLSCLAWP*CCCGCSADLLLPS
DTWKSLPCSAPQLPTIWFLLHGRASDSHFE*TR
HRTCVLRIIW
Frame 2
EAWLVLTYNN*LLSGLRPIPGSTPGLHSHSLPVS*ATKPQLSPGIRQGGV*SRAEA*ARRRATSSP
RDRHGSADDNTAAAPSSVGGCSRGGSDKDCMGQ
D*ASQCLHERQAPQGKARPRGQVA*AVSTLEEEC
LLFYQHQPGSP*GCFLPI*IQLEPLWRDGTCLQ
TAFHPGHLPLRVLPQLGALDPAGGSELAQRAGT
ERAPVQRGL*AMVGRLSHLLHLQEQLAQGLELDF
RV*QVRSGSCLPTFPFLLPHTHCSVQ*NLDSLL
QGQQLQPREWPLHPDVVRPSPGQPQ*GGGEVLC
CSHEWGWALGSLAFPA*PGPNAAVAAQLTSFYLL
IPGNPCPVQPHSSQLFGSCSMVGPLTATLNKPD
TAHVS*ELF
Sequence NM_016724
TGGAGGCCTGGCTGGTGCTCACATACAATAATTAACTGCTGAGTGGCCTTCGCCCAATCCCAGGCTCCACTCCTGGGCTCCATTCCCACTCCCTGCCTGTCTCCTAGGCCACTAAACCACAGCTGTCCCCTGGAATAAGGCAAGGGGGAGTGTAGAGCAGAGCAGAAGCCTGAGCCAGACGGAGAGCCACCTCCTCTCCC
AGGAACTGAACCCAAAGGATCACCTGGTATTCCCTGAGAGTACAGATTTCTCCGGCGTGGCCCTCAAGGGACAGACATGGCTCAGCGGATGACAACACAG
CTGCTGCTCCTTCTAGTGTGGGTGGCTGTAGTAGGGGAGGCTCAGACAAGGATTGCATGGGCCAGGACTGAGCTTCTCAATGTCTGCATGAACGCCAAGC
ACCACAAGGAAAAGCCAGGCCCCGAGGACAAGTTGCATGAGCAGTGTCGACCCTGGAGGAAGAATGCCTGCTGTTCTACCAACACCAGCCAGGAAGCCCA
TAAGGATGTTTCCTACCTATATAGATTCAACTGGAACCACTGTGGAGAGATGGCACCTGCCTGCAAACGGCATTTCATCCAGGACACCTGCCTCTACGAG
TGCTCCCCCAACTTGGGGCCCTGGATCCAGCAGGTGGATCAGAGCTGGCGCAAAGAGCGGGTACTGAACGTGCCCCTGTGCAAAGAGGACTGTGAGCAAT
GGTGGGAAGATTGTCGCACCTCCTACACCTGCAAGAGCAACTGGCACAAGGGCTGGAACTGGACTTCAGGGTTTAACAAGTGCGCAGTGGGAGCTGCCTG
CCAACCTTTCCATTTCTACTTCCCCACACCCACTGTTCTGTGCAATGAAATCTGGACTCACTCCTACAAGGTCAGCAACTACAGCCGAGGGAGTGGCCGC
TGCATCCAGATGTGGTTCGACCCAGCCCAGGGCAACCCCAATGAGGAGGTGGCGAGGTTCTATGCTGCAGCCATGAGTGGGGCTGGGCCCTGGGCAGCCT
GGCCTTTCCTGCTTAGCCTGGCCCTAATGCTGCTGTGGCTGCTCAGCTGACCTCCTTTTACCTTCTGATACCTGGAAATCCCTGCCCTGTTCAGCCCCAC
AGCTCCCAACTATTTGGTTCCTGCTCCATGGTCGGGCCTCTGACAGCCACTTTGAATAAACCAGACACCGCACATGTGTCTTGAGAATTATTTGG
- refseq_FOLR1.F1 (forward primer)
- refseq_FOLR1.R1 (reverse primer)
- Single exon skipping
Frame 0
WRPGWCSHTIINC*VAFAQSQAPLLGSIPTPCLSPRPLNHSCPLE*GKGECRAEQKPEPDGEPPPLP
GTEPKGSPGIP*EYRFLRRGPQGTDMAQRMTTQ
LLLLLVWVAVVGEAQTRIAWARTELLNVCMNAK
HHKEKPGPEDKLHEQCRPWRKNACCSTNTSQEAH
KDVSYLYRFNWNHCGEMAPACKRHFIQDTCLYE
CSPNLGPWIQQVDQSWRKERVLNVPLCKEDCEQ
WWEDCRTSYTCKSNWHKGWNWTSGFNKCAVGAAC
QPFHFYFPTPTVLCNEIWTHSYKVSNYSRGSGR
CIQMWFDPAQGNPNEEVARFYAAAMSGAGPWAA
WPFLLSLALMLLWLLS*PPFTF*YLEIPALFSPT
APNYLVPAPWSGL*QPL*INQTPHMCLENYL
Frame 1
GGLAGAHIQ*LTAEWPSPNPRLHSWAPFPLPACLLGH*TTAVPWNKARGSVEQSRSLSQTESHLLS
QELNPKDHLVFPESTDFSGVALKGQTWLSG*QHS
CCSF*CGWL**GRLRQGLHGPGLSFSMSA*TPS
TTRKSQAPRTSCMSSVDPGGRMPAVLPTPARKP
IRMFPTYIDSTGTTVERWHLPANGISSRTPASTS
APPTWGPGSSRWIRAGAKSGY*TCPCAKRTVSN
GGKIVAPPTPARATGTRAGTGLQGLTSAQWELP
ANLSISTSPHPLFCAMKSGLTPTRSATTAEGVAA
ASRCGSTQPRATPMRRWRGSMLQP*VGLGPGQP
GLSCLAWP*CCCGCSADLLLPSDTWKSLPCSAP
QLPTIWFLLHGRASDSHFE*TRHRTCVLRIIW
Frame 2
EAWLVLTYNN*LLSGLRPIPGSTPGLHSHSLPVS*ATKPQLSPGIRQGGV*SRAEA*ARRRATSSP
RN*TQRITWYSLRVQISPAWPSRDRHGSADDNT
AAAPSSVGGCSRGGSDKDCMGQD*ASQCLHERQA
PQGKARPRGQVA*AVSTLEEECLLFYQHQPGSP
*GCFLPI*IQLEPLWRDGTCLQTAFHPGHLPLR
VLPQLGALDPAGGSELAQRAGTERAPVQRGL*AM
VGRLSHLLHLQEQLAQGLELDFRV*QVRSGSCL
PTFPFLLPHTHCSVQ*NLDSLLQGQQLQPREWP
LHPDVVRPSPGQPQ*GGGEVLCCSHEWGWALGSL
AFPA*PGPNAAVAAQLTSFYLLIPGNPCPVQPH
SSQLFGSCSMVGPLTATLNKPDTAHVS*ELF
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