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Sequences

Sequence NM_006724

AAGATGGCCGCGGCGCGCACGGCTCCTGCGGCGGGGTAGAGGCGGAGGCGGAGTCGAGTCACTCCCGCACTTCGGGGCTCCGGTGCCCCGCGCCAGGCTG CAGCTTACTGCCCGCCGCGGCCATGCGGGGCTCCGTGCACGGATGAGAGAAGCCGCTGCCGCGCTGGTCCCTCCTCCCGCCTTTGCCGTCACGCCTGCCG CCGCCATGGAGGAGCCGCCGCCACCGCCGCCGCCGCCACCACCGCCACCGGAACCCGAGACCGAGTCAGAACCCGAGTGCTGCTTGGCGGCGAGGCAAGA GGGCACATTGGGAGATTCAGCTTGCAAGAGTCCTGAATCTGATCTAGAAGACTTCTCCGATGAAACAAATACAGAGAATCTTTATGGTACCTCTCCCCCC AGCACACCTCGACAGATGAAACGCATGTCAACCAAACATCAGAGGAATAATGTGGGGAGGCCAGCCAGTCGGTCTAATTTGAAAGAAAAAATGAATGCAC CAAATCAGCCTCCACATAAAGACACTGGAAAAACAGTGGAGAATGTGGAAGAATACAGCTATAAGCAGGAGAAAAAGATCCGAGCAGCTCTTAGAACAAC AGAGCGTGATCGTAAAAAAAATGTACAGTGCTCATTCATGTTAGACTCAGTGGGTGGATCTTTGCCAAAAAAATCAATTCCAGATGTGGATCTCAATAAG CCTTACCTCAGCCTTGGCTGTAGCAATGCTAAGCTTCCAGTATCTGTGCCCATGCCTATAGCCAGACCTGCACGCCAGACTTCTAGGACTGACTGTCCAG CAGATCGTTTAAAGTTTTTTGAAACTTTACGACTTTTGCTAAAGCTTACCTCAGTCTCAAAGAAAAAAGACAGGGAGCAAAGAGGACAAGAAAATACGTC TGGTTTCTGGCTTAACCGATCTAACGAACTGATCTGGTTAGAGCTACAAGCCTGGCATGCAGGACGGACAATTAACGACCAGGACTTCTTTTTATATACA GCCCGTCAAGCCATCCCAGATATTATTAATGAAATCCTTACTTTCAAAGTCGACTATGGGAGCTTCGCCTTTGTTAGAGATAGAGCTGGTTTTAATGGTA CTTCAGTAGAAGGGCAGTGCAAAGCCACTCCTGGAACAAAGATTGTAGGTTACTCAACACATCATGAGCATCTCCAACGCCAGAGGGTCTCATTTGAGCA GGTAAAACGGATAATGGAGCTGCTAGAGTACATAGAAGCACTTTATCCATCATTGCAGGCTCTTCAGAAGGACTATGAAAAATATGCTGCAAAAGACTTC CAGGACAGGGTGCAGGCACTCTGTTTGTGGTTAAACATCACAAAAGACTTAAATCAGAAATTAAGGATTATGGGCACTGTTTTGGGCATCAAGAATTTAT CAGACATTGGCTGGCCAGTGTTTGAAATCCCTTCCCCTCGACCATCCAAAGGTAATGAGCCGGAGTATGAGGGTGATGACACAGAAGGAGAATTAAAGGA GTTGGAAAGTAGTACGGATGAGAGTGAAGAAGAACAAATCTCTGATCCTAGGGTACCGGAAATCAGACAGCCCATAGATAACAGCTTCGACATCCAGTCG CGGGACTGCATATCCAAGAAGCTTGAGAGGCTCGAATCTGAGGATGATTCTCTTGGCTGGGGAGCACCAGACTGGAGCACAGAAGCAGGCTTTAGTAGAC ATTGTCTGACTTCTATTTATAGACCATTTGTAGACAAAGCACTGAAGCAGATGGGGTTAAGAAAGTTAATTTTAAGACTTCACAAGCTAATGGATGGTTC CTTGCAAAGGGCACGTATAGCATTGGTAAAGAACGATCGTCCAGTGGAGTTTTCTGAATTTCCAGATCCCATGTGGGGTTCAGATTATGTGCAGTTGTCA AGGACACCACCTTCATCTGAGGAGAAATGCAGTGCTGTGTCGTGGGAGGAGCTGAAGGCCATGGATTTACCTTCATTCGAACCTGCCTTCCTAGTTCTCT GCCGAGTCCTTCTGAATGTCATACATGAGTGTCTGAAGTTAAGATTGGAGCAGAGACCTGCTGGAGAACCATCTCTCTTGAGTATTAAGCAGCTGGTGAG AGAGTGTAAGGAGGTCCTGAAGGGCGGCCTGCTGATGAAGCAGTACTACCAGTTCATGCTGCAGGAGGTTCTGGAGGACTTGGAGAAGCCCGACTGCAAC ATTGACGCTTTTGAAGAGGATCTACATAAAATGCTTATGGTGTATTTTGATTACATGAGAAGCTGGATCCAAATGCTACAGCAATTACCTCAAGCATCGC ATAGTTTAAAAAATCTGTTAGAAGAAGAATGGAATTTCACCAAAGAAATAACTCATTACATACGGGGAGGAGAAGCACAGGCCGGGAAGCTTTTCTGTGA CATTGCAGGAATGCTGCTGAAATCTACAGGAAGTTTTTTAGAATTTGGCTTACAGGAGAGCTGTGCTGAATTTTGGACTAGTGCGGATGACAGCAGTGCT TCCGACGAAATCAGGAGGTCTGTTATAGAGATCAGTCGAGCCCTGAAGGAGCTCTTCCATGAAGCCAGAGAAAGGGCTTCCAAAGCACTTGGATTTGCTA AAATGTTGAGAAAGGACCTGGAAATAGCAGCAGAATTCAGGCTTTCAGCCCCAGTTAGAGACCTCCTGGATGTTCTGAAATCAAAACAGTATGTCAAGGT GCAAATTCCTGGGTTAGAAAACTTGCAAATGTTTGTTCCAGACACTCTTGCTGAGGAGAAGAGTATTATTTTGCAGTTACTCAATGCAGCTGCAGGAAAG GACTGTTCAAAAGATTCAGATGACGTACTCATCGATGCCTATCTGCTTCTGACCAAGCACGGTGATCGAGCCCGTGATTCAGAGGACAGCTGGGGCACCT GGGAGGCACAGCCTGTCAAAGTCGTGCCTCAGGTGGAGACTGTTGACACCCTGAGAAGCATGCAGGTGGATAATCTTTTACTAGTTGTCATGCAGTCTGC GCATCTCACAATTCAGAGAAAAGCTTTCCAGCAGTCCATTGAGGGACTTATGACTCTGTGCCAGGAGCAGACATCCAGTCAGCCGGTCATCGCCAAAGCT TTGCAGCAGCTGAAGAATGATGCATTGGAGCTATGCAACAGGATAAGCAATGCCATTGACCGCGTGGACCACATGTTCACATCAGAATTTGATGCTGAGG TTGATGAATCTGAATCTGTCACCTTGCAACAGTACTACCGAGAAGCAATGATTCAGGGGTACAATTTTGGATTTGAGTATCATAAAGAAGTTGTTCGTTT GATGTCTGGGGAGTTTAGACAGAAGATAGGAGACAAATATATAAGCTTTGCCCGGAAGTGGATGAATTATGTCCTGACTAAATGTGAGAGTGGTAGAGGT ACAAGACCCAGGTGGGCGACTCAAGGATTTGATTTTCTACAAGCAATTGAACCTGCCTTTATTTCAGCTTTACCAGAAGATGACTTCTTGAGTTTACAAG CCTTGATGAATGAATGCATTGGCCATGTCATAGGAAAACCACACAGTCCTGTTACAGGTTTGTACCTTGCCATTCATCGGAACAGCCCCCGTCCTATGAA GGTACCTCGATGCCATAGTGACCCTCCTAACCCACACCTCATTATCCCCACTCCAGAGGGATTCAGGGGTTCCAGCGTTCCTGAAAATGATCGATTGGCT TCCATAGCTGCTGAATTGCAGTTTAGGTCCCTGAGTCGTCACTCAAGCCCCACGGAGGAGCGAGATGAACCAGCATATCCAAGAGGAGATTCAAGTGGGT CCACAAGAAGAAGTTGGGAACTTCGGACACTAATCAGCCAGAGTAAAGATACTGCTTCTAAACTAGGACCCATAGAAGCTATCCAGAAGTCAGTCCGATT GTTTGAAGAAAAGAGGTACCGAGAAATGAGGAGAAAGAATATCATTGGTCAAGTTTGTGATACGCCTAAGTCCTATGATAATGTTATGCACGTTGGCTTG AGGAAGGTGACCTTCAAATGGCAAAGAGGAAACAAAATTGGAGAAGGCCAGTATGGGAAGGTGTACACCTGCATCAGCGTCGACACCGGGGAGCTGATGG CCATGAAAGAGATTCGATTTCAACCTAATGACCATAAGACTATCAAGGAAACTGCAGACGAATTGAAAATATTCGAAGGCATCAAACACCCCAATCTGGT TCGGTATTTTGGTGTGGAGCTCCATAGAGAAGAAATGTACATCTTCATGGAGTACTGCGATGAGGGGACTTTAGAAGAGGTGTCAAGGCTGGGACTTCAG GAACATGTGATTAGGCTGTATTCAAAGCAGATCACCATTGCGATCAACGTCCTCCATGAGCATGGCATAGTCCACCGTGACATTAAAGGTGCCAATATCT TCCTTACCTCATCTGGATTAATCAAACTGGGAGATTTTGGATGTTCAGTAAAGCTCAAAAACAATGCCCAGACCATGCCTGGTGAAGTGAACAGCACCCT GGGGACAGCAGCATACATGGCACCTGAAGTCATCACTCGTGCCAAAGGAGAGGGCCATGGGCGTGCGGCCGACATCTGGAGTCTGGGGTGTGTTGTCATA GAGATGGTGACTGGCAAGAGGCCTTGGCATGAGTATGAGCACAACTTTCAAATTATGTATAAAGTGGGGATGGGACATAAGCCACCAATCCCTGAAAGAT TAAGCCCTGAAGGAAAGGACTTCCTTTCTCACTGCCTTGAGAGTGACCCAAAGATGAGATGGACCGCCAGCCAGCTCCTCGACCATTCGTTTGTCAAGGT TTGCACAGATGAAGAATGAAGCCTAGTAGAATATGGACTTGGAAAATTCTCTTAATCACTACTGTATGTAATATTTACATAAAGACTGTGCTGAGAAGCA GTATAAGCCTTTTTAACCTTCCAAGACTGAAGACTGCACAGGTGACAAGCGTCACTTCTCCTGCTGCTCCTGTTTGTCTGATGTGGCAAAAGGCCCTCTG GAGGGCTGGTGGCCACGAGGTTAAAGAAGCTGCATGTTAAGTGCCATTACTACTGTACACGGACCATCGCCTCTGTCTCCTCCGTGTCTCGCGCGACTGA GAACCGTGACATCAGCGTAGTGTTTTGACCTTTCTAGGTTCAAAAGAAGTTGTAGTGTTATCAGGCGTCCCATACCTTGTTTTTAATCTCCTGTTTGTTG AGTGCACTGACTGTGAAACCTTTACCTTTTTTGTTGTTGTTGGCAAGCTGCAGGTTTGTAATGCAAAAGGCTGATTACTGAAATTTAAGAAAAAGGTTCT TTTTTCAATAAATGGTTTATTTTAGGAAAGCTCA

refseq_MAP3K4.F1 (forward primer)
refseq_MAP3K4.R1 (reverse primer)
Single exon skipping

Frame 0

KMAAARTAPAAG*RRRRSRVTPALRGSGAPRQA AAYCPPRPCGAPCTDERSRCRAGPSSRLCRHACR RHGGAAATAAAATTATGTRDRVRTRVLLGGEAR GHIGRFSLQES*I*SRRLLR*NKYRESLWYLSP QHTSTDETHVNQTSEE*CGEASQSV*FERKNECT KSAST*RHWKNSGECGRIQL*AGEKDPSSS*NN RA*S*KKCTVLIHVRLSGWIFAKKINSRCGSQ* ALPQPWL*QC*ASSICAHAYSQTCTPDF*D*LSS RSFKVF*NFTTFAKAYLSLKEKRQGAKRTRKYV WFLA*PI*RTDLVRATSLACRTDN*RPGLLFIY SPSSHPRYY**NPYFQSRLWELRLC*R*SWF*WY FSRRAVQSHSWNKDCRLLNTS*ASPTPEGLI*A GKTDNGAARVHRSTLSIIAGSSEGL*KICCKRL PGQGAGTLFVVKHHKRLKSEIKDYGHCFGHQEFI RHWLASV*NPFPSTIQR**AGV*G**HRRRIKG VGK*YG*E*RRTNL*S*GTGNQTAHR*QLRHPV AGLHIQEA*EARI*G*FSWLGSTRLEHRSRL**T LSDFYL*TICRQSTEADGVKKVNFKTSQANGWF LAKGTYSIGKERSSSGVF*ISRSHVGFRLCAVV KDTTFI*GEMQCCVVGGAEGHGFTFIRTCLPSSL PSPSECHT*VSEVKIGAETCWRTISLEY*AAGE RV*GGPEGRPADEAVLPVHAAGGSGGLGEARLQ H*RF*RGST*NAYGVF*LHEKLDPNATAITSSIA *FKKSVRRRMEFHQRNNSLHTGRRSTGREAFL* HCRNAAEIYRKFFRIWLTGELC*ILD*CG*QQC FRRNQEVCYRDQSSPEGALP*SQRKGFQSTWIC* NVEKGPGNSSRIQAFSPS*RPPGCSEIKTVCQG ANSWVRKLANVCSRHSC*GEEYYFAVTQCSCRK GLFKRFR*RTHRCLSASDQAR*SSP*FRGQLGHL GGTACQSRASGGDC*HPEKHAGG*SFTSCHAVC ASHNSEKSFPAVH*GTYDSVPGADIQSAGHRQS FAAAEE*CIGAMQQDKQCH*PRGPHVHIRI*C*G **I*ICHLATVLPRSNDSGVQFWI*VS*RSCSF DVWGV*TEDRRQIYKLCPEVDELCPD*M*EW*R YKTQVGDSRI*FSTSN*TCLYFSFTRR*LLEFTS LDE*MHWPCHRKTTQSCYRFVPCHSSEQPPSYE GTSMP**PS*PTPHYPHSRGIQGFQRS*K*SIG FHSC*IAV*VPESSLKPHGGAR*TSISKRRFKWV HKKKLGTSDTNQPE*RYCF*TRTHRSYPEVSPI V*RKEVPRNEEKEYHWSSL*YA*VL**CYARWL EEGDLQMAKRKQNWRRPVWEGVHLHQRRHRGADG HERDSIST**P*DYQGNCRRIENIRRHQTPQSG SVFWCGAP*RRNVHLHGVLR*GDFRRGVKAGTS GTCD*AVFKADHHCDQRPP*AWHSPP*H*RCQYL PYLIWINQTGRFWMFSKAQKQCPDHAW*SEQHP GDSSIHGT*SHHSCQRRGPWACGRHLESGVCCH RDGDWQEALA*V*AQLSNYV*SGDGT*ATNP*KI KP*RKGLPFSLP*E*PKDEMDRQPAPRPFVCQG LHR*RMKPSRIWTWKILLITTVCNIYIKTVLRS SISLFNLPRLKTAQVTSVTSPAAPVCLMWQKALW RAGGHEVKEAAC*VPLLLYTDHRLCLLRVSRD* EP*HQRSVLTFLGSKEVVVLSGVPYLVFNLLFV ECTDCETFTFFVVVGKLQVCNAKG*LLKFKKKVL FSINGLF*ESS

Frame 1

RWPRRARLLRRGRGGGGVESLPHFGAPVPRARL QLTARRGHAGLRARMREAAAALVPPPAFAVTPA AAMEEPPPPPPPPPPPPEPETESEPECCLAARQE GTLGDSACKSPESDLEDFSDETNTENLYGTSPP STPRQMKRMSTKHQRNNVGRPASRSNLKEKMNA PNQPPHKDTGKTVENVEEYSYKQEKKIRAALRTT ERDRKKNVQCSFMLDSVGGSLPKKSIPDVDLNK PYLSLGCSNAKLPVSVPMPIARPARQTSRTDCP ADRLKFFETLRLLLKLTSVSKKKDREQRGQENTS GFWLNRSNELIWLELQAWHAGRTINDQDFFLYT ARQAIPDIINEILTFKVDYGSFAFVRDRAGFNG TSVEGQCKATPGTKIVGYSTHHEHLQRQRVSFEQ VKRIMELLEYIEALYPSLQALQKDYEKYAAKDF QDRVQALCLWLNITKDLNQKLRIMGTVLGIKNL SDIGWPVFEIPSPRPSKGNEPEYEGDDTEGELKE LESSTDESEEEQISDPRVPEIRQPIDNSFDIQS RDCISKKLERLESEDDSLGWGAPDWSTEAGFSR HCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGS LQRARIALVKNDRPVEFSEFPDPMWGSDYVQLS RTPPSSEEKCSAVSWEELKAMDLPSFEPAFLVL CRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVR ECKEVLKGGLLMKQYYQFMLQEVLEDLEKPDCN IDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQAS HSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCD IAGMLLKSTGSFLEFGLQESCAEFWTSADDSSA SDEIRRSVIEISRALKELFHEARERASKALGFA KMLRKDLEIAAEFRLSAPVRDLLDVLKSKQYVKV QIPGLENLQMFVPDTLAEEKSIILQLLNAAAGK DCSKDSDDVLIDAYLLLTKHGDRARDSEDSWGT WEAQPVKVVPQVETVDTLRSMQVDNLLLVVMQSA HLTIQRKAFQQSIEGLMTLCQEQTSSQPVIAKA LQQLKNDALELCNRISNAIDRVDHMFTSEFDAE VDESESVTLQQYYREAMIQGYNFGFEYHKEVVRL MSGEFRQKIGDKYISFARKWMNYVLTKCESGRG TRPRWATQGFDFLQAIEPAFISALPEDDFLSLQ ALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPMK VPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLA SIAAELQFRSLSRHSSPTEERDEPAYPRGDSSG STRRSWELRTLISQSKDTASKLGPIEAIQKSVRL FEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGL RKVTFKWQRGNKIGEGQYGKVYTCISVDTGELM AMKEIRFQPNDHKTIKETADELKIFEGIKHPNLV RYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQ EHVIRLYSKQITIAINVLHEHGIVHRDIKGANI FLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTL GTAAYMAPEVITRAKGEGHGRAADIWSLGCVVI EMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPER LSPEGKDFLSHCLESDPKMRWTASQLLDHSFVKV CTDEE*SLVEYGLGKFS*SLLYVIFT*RLC*EA V*AFLTFQD*RLHR*QASLLLLLLFV*CGKRPS GGLVATRLKKLHVKCHYYCTRTIASVSSVSRATE NRDISVVF*PF*VQKKL*CYQASHTLFLISCLL SALTVKPLPFLLLLASCRFVMQKADY*NLRKRF FFQ*MVYFRKA

Frame 2

DGRGAHGSCGGVEAEAESSHSRTSGLRCPAPGC SLLPAAAMRGSVHG*EKPLPRWSLLPPLPSRLP PPWRSRRHRRRRHHRHRNPRPSQNPSAAWRRGK RAHWEIQLARVLNLI*KTSPMKQIQRIFMVPLPP AHLDR*NACQPNIRGIMWGGQPVGLI*KKK*MH QISLHIKTLEKQWRMWKNTAISRRKRSEQLLEQ QSVIVKKMYSAHSC*TQWVDLCQKNQFQMWISIS LTSALAVAMLSFQYLCPCL*PDLHARLLGLTVQ QIV*SFLKLYDFC*SLPQSQRKKTGSKEDKKIR LVSGLTDLTN*SG*SYKPGMQDGQLTTRTSFYIQ PVKPSQILLMKSLLSKSTMGASPLLEIELVLMV LQ*KGSAKPLLEQRL*VTQHIMSISNARGSHLS R*NG*WSC*ST*KHFIHHCRLFRRTMKNMLQKTS RTGCRHSVCG*TSQKT*IRN*GLWALFWASRIY QTLAGQCLKSLPLDHPKVMSRSMRVMTQKEN*R SWKVVRMRVKKNKSLILGYRKSDSP*ITASTSSR GTAYPRSLRGSNLRMILLAGEHQTGAQKQALVD IV*LLFIDHL*TKH*SRWG*ES*F*DFTS*WMV PCKGHV*HW*RTIVQWSFLNFQIPCGVQIMCSCQ GHHLHLRRNAVLCRGRS*RPWIYLHSNLPS*FS AESF*MSYMSV*S*DWSRDLLENHLS*VLSSW* ESVRRS*RAAC**SSTTSSCCRRFWRTWRSPTAT LTLLKRIYIKCLWCILIT*EAGSKCYSNYLKHR IV*KIC*KKNGISPKK*LITYGEEKHRPGSFSV TLQECC*NLQEVF*NLAYRRAVLNFGLVRMTAVL PTKSGGLL*RSVEP*RSSSMKPEKGLPKHLDLL KC*ERTWK*QQNSGFQPQLETSWMF*NQNSMSR CKFLG*KTCKCLFQTLLLRRRVLFCSYSMQLQER TVQKIQMTYSSMPICF*PSTVIEPVIQRTAGAP GRHSLSKSCLRWRLLTP*EACRWIIFY*LSCSL RISQFREKLSSSPLRDL*LCARSRHPVSRSSPKL CSS*RMMHWSYATG*AMPLTAWTTCSHQNLMLR LMNLNLSPCNSTTEKQ*FRGTILDLSIIKKLFV *CLGSLDRR*ETNI*ALPGSG*IMS*LNVRVVEV QDPGGRLKDLIFYKQLNLPLFQLYQKMTS*VYK P**MNALAMS*ENHTVLLQVCTLPFIGTAPVL* RYLDAIVTLLTHTSLSPLQRDSGVPAFLKMIDWL P*LLNCSLGP*VVTQAPRRSEMNQHIQEEIQVG PQEEVGNFGH*SARVKILLLN*DP*KLSRSQSD CLKKRGTEK*GERISLVKFVIRLSPMIMLCTLA* GR*PSNGKEETKLEKASMGRCTPASASTPGS*W P*KRFDFNLMTIRLSRKLQTN*KYSKASNTPIW FGILVWSSIEKKCTSSWSTAMRGL*KRCQGWDFR NM*LGCIQSRSPLRSTSSMSMA*STVTLKVPIS SLPHLD*SNWEILDVQ*SSKTMPRPCLVK*TAP WGQQHTWHLKSSLVPKERAMGVRPTSGVWGVLS* RW*LARGLGMSMSTTFKLCIKWGWDISHQSLKD *ALKERTSFLTALRVTQR*DGPPASSSTIRLSR FAQMKNEA**NMDLENSLNHYCM*YLHKDCAEKQ YKPF*PSKTEDCTGDKRHFSCCSCLSDVAKGPL EGWWPRG*RSCMLSAITTVHGPSPLSPPCLARL RTVTSA*CFDLSRFKRSCSVIRRPIPCF*SPVC* VH*L*NLYLFCCCWQAAGL*CKRLITEI*EKGS FFNKWFILGKL

Sequence NM_005922

AAGATGGCCGCGGCGCGCACGGCTCCTGCGGCGGGGTAGAGGCGGAGGCGGAGTCGAGTCACTCCCGCACTTCGGGGCTCCGGTGCCCCGCGCCAGGCTG CAGCTTACTGCCCGCCGCGGCCATGCGGGGCTCCGTGCACGGATGAGAGAAGCCGCTGCCGCGCTGGTCCCTCCTCCCGCCTTTGCCGTCACGCCTGCCG CCGCCATGGAGGAGCCGCCGCCACCGCCGCCGCCGCCACCACCGCCACCGGAACCCGAGACCGAGTCAGAACCCGAGTGCTGCTTGGCGGCGAGGCAAGA GGGCACATTGGGAGATTCAGCTTGCAAGAGTCCTGAATCTGATCTAGAAGACTTCTCCGATGAAACAAATACAGAGAATCTTTATGGTACCTCTCCCCCC AGCACACCTCGACAGATGAAACGCATGTCAACCAAACATCAGAGGAATAATGTGGGGAGGCCAGCCAGTCGGTCTAATTTGAAAGAAAAAATGAATGCAC CAAATCAGCCTCCACATAAAGACACTGGAAAAACAGTGGAGAATGTGGAAGAATACAGCTATAAGCAGGAGAAAAAGATCCGAGCAGCTCTTAGAACAAC AGAGCGTGATCGTAAAAAAAATGTACAGTGCTCATTCATGTTAGACTCAGTGGGTGGATCTTTGCCAAAAAAATCAATTCCAGATGTGGATCTCAATAAG CCTTACCTCAGCCTTGGCTGTAGCAATGCTAAGCTTCCAGTATCTGTGCCCATGCCTATAGCCAGACCTGCACGCCAGACTTCTAGGACTGACTGTCCAG CAGATCGTTTAAAGTTTTTTGAAACTTTACGACTTTTGCTAAAGCTTACCTCAGTCTCAAAGAAAAAAGACAGGGAGCAAAGAGGACAAGAAAATACGTC TGGTTTCTGGCTTAACCGATCTAACGAACTGATCTGGTTAGAGCTACAAGCCTGGCATGCAGGACGGACAATTAACGACCAGGACTTCTTTTTATATACA GCCCGTCAAGCCATCCCAGATATTATTAATGAAATCCTTACTTTCAAAGTCGACTATGGGAGCTTCGCCTTTGTTAGAGATAGAGCTGGTTTTAATGGTA CTTCAGTAGAAGGGCAGTGCAAAGCCACTCCTGGAACAAAGATTGTAGGTTACTCAACACATCATGAGCATCTCCAACGCCAGAGGGTCTCATTTGAGCA GGTAAAACGGATAATGGAGCTGCTAGAGTACATAGAAGCACTTTATCCATCATTGCAGGCTCTTCAGAAGGACTATGAAAAATATGCTGCAAAAGACTTC CAGGACAGGGTGCAGGCACTCTGTTTGTGGTTAAACATCACAAAAGACTTAAATCAGAAATTAAGGATTATGGGCACTGTTTTGGGCATCAAGAATTTAT CAGACATTGGCTGGCCAGTGTTTGAAATCCCTTCCCCTCGACCATCCAAAGGTAATGAGCCGGAGTATGAGGGTGATGACACAGAAGGAGAATTAAAGGA GTTGGAAAGTAGTACGGATGAGAGTGAAGAAGAACAAATCTCTGATCCTAGGGTACCGGAAATCAGACAGCCCATAGATAACAGCTTCGACATCCAGTCG CGGGACTGCATATCCAAGAAGCTTGAGAGGCTCGAATCTGAGGATGATTCTCTTGGCTGGGGAGCACCAGACTGGAGCACAGAAGCAGGCTTTAGTAGAC ATTGTCTGACTTCTATTTATAGACCATTTGTAGACAAAGCACTGAAGCAGATGGGGTTAAGAAAGTTAATTTTAAGACTTCACAAGCTAATGGATGGTTC CTTGCAAAGGGCACGTATAGCATTGGTAAAGAACGATCGTCCAGTGGAGTTTTCTGAATTTCCAGATCCCATGTGGGGTTCAGATTATGTGCAGTTGTCA AGGACACCACCTTCATCTGAGGAGAAATGCAGTGCTGTGTCGTGGGAGGAGCTGAAGGCCATGGATTTACCTTCATTCGAACCTGCCTTCCTAGTTCTCT GCCGAGTCCTTCTGAATGTCATACATGAGTGTCTGAAGTTAAGATTGGAGCAGAGACCTGCTGGAGAACCATCTCTCTTGAGTATTAAGCAGCTGGTGAG AGAGTGTAAGGAGGTCCTGAAGGGCGGCCTGCTGATGAAGCAGTACTACCAGTTCATGCTGCAGGAGGTTCTGGAGGACTTGGAGAAGCCCGACTGCAAC ATTGACGCTTTTGAAGAGGATCTACATAAAATGCTTATGGTGTATTTTGATTACATGAGAAGCTGGATCCAAATGCTACAGCAATTACCTCAAGCATCGC ATAGTTTAAAAAATCTGTTAGAAGAAGAATGGAATTTCACCAAAGAAATAACTCATTACATACGGGGAGGAGAAGCACAGGCCGGGAAGCTTTTCTGTGA CATTGCAGGAATGCTGCTGAAATCTACAGGAAGTTTTTTAGAATTTGGCTTACAGGAGAGCTGTGCTGAATTTTGGACTAGTGCGGATGACAGCAGTGCT TCCGACGAAATCAGGAGGTCTGTTATAGAGATCAGTCGAGCCCTGAAGGAGCTCTTCCATGAAGCCAGAGAAAGGGCTTCCAAAGCACTTGGATTTGCTA AAATGTTGAGAAAGGACCTGGAAATAGCAGCAGAATTCAGGCTTTCAGCCCCAGTTAGAGACCTCCTGGATGTTCTGAAATCAAAACAGTATGTCAAGGT GCAAATTCCTGGGTTAGAAAACTTGCAAATGTTTGTTCCAGACACTCTTGCTGAGGAGAAGAGTATTATTTTGCAGTTACTCAATGCAGCTGCAGGAAAG GACTGTTCAAAAGATTCAGATGACGTACTCATCGATGCCTATCTGCTTCTGACCAAGCACGGTGATCGAGCCCGTGATTCAGAGGACAGCTGGGGCACCT GGGAGGCACAGCCTGTCAAAGTCGTGCCTCAGGTGGAGACTGTTGACACCCTGAGAAGCATGCAGGTGGATAATCTTTTACTAGTTGTCATGCAGTCTGC GCATCTCACAATTCAGAGAAAAGCTTTCCAGCAGTCCATTGAGGGACTTATGACTCTGTGCCAGGAGCAGACATCCAGTCAGCCGGTCATCGCCAAAGCT TTGCAGCAGCTGAAGAATGATGCATTGGAGCTATGCAACAGGATAAGCAATGCCATTGACCGCGTGGACCACATGTTCACATCAGAATTTGATGCTGAGG TTGATGAATCTGAATCTGTCACCTTGCAACAGTACTACCGAGAAGCAATGATTCAGGGGTACAATTTTGGATTTGAGTATCATAAAGAAGTTGTTCGTTT GATGTCTGGGGAGTTTAGACAGAAGATAGGAGACAAATATATAAGCTTTGCCCGGAAGTGGATGAATTATGTCCTGACTAAATGTGAGAGTGGTAGAGGT ACAAGACCCAGGTGGGCGACTCAAGGATTTGATTTTCTACAAGCAATTGAACCTGCCTTTATTTCAGCTTTACCAGAAGATGACTTCTTGAGTTTACAAG CCTTGATGAATGAATGCATTGGCCATGTCATAGGAAAACCACACAGTCCTGTTACAGGTTTGTACCTTGCCATTCATCGGAACAGCCCCCGTCCTATGAA GGTACCTCGATGCCATAGTGACCCTCCTAACCCACACCTCATTATCCCCACTCCAGAGGGATTCAGCACTCGGAGCATGCCTTCCGACGCGCGGAGCCAT GGCAGCCCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGTTGCTGCCAGTCGGCCCAGCCCCTCTGGTGGTGACTCTGTGCTGCCCAAATCCATCAGCA GTGCCCATGATACCAGGGGTTCCAGCGTTCCTGAAAATGATCGATTGGCTTCCATAGCTGCTGAATTGCAGTTTAGGTCCCTGAGTCGTCACTCAAGCCC CACGGAGGAGCGAGATGAACCAGCATATCCAAGAGGAGATTCAAGTGGGTCCACAAGAAGAAGTTGGGAACTTCGGACACTAATCAGCCAGAGTAAAGAT ACTGCTTCTAAACTAGGACCCATAGAAGCTATCCAGAAGTCAGTCCGATTGTTTGAAGAAAAGAGGTACCGAGAAATGAGGAGAAAGAATATCATTGGTC AAGTTTGTGATACGCCTAAGTCCTATGATAATGTTATGCACGTTGGCTTGAGGAAGGTGACCTTCAAATGGCAAAGAGGAAACAAAATTGGAGAAGGCCA GTATGGGAAGGTGTACACCTGCATCAGCGTCGACACCGGGGAGCTGATGGCCATGAAAGAGATTCGATTTCAACCTAATGACCATAAGACTATCAAGGAA ACTGCAGACGAATTGAAAATATTCGAAGGCATCAAACACCCCAATCTGGTTCGGTATTTTGGTGTGGAGCTCCATAGAGAAGAAATGTACATCTTCATGG AGTACTGCGATGAGGGGACTTTAGAAGAGGTGTCAAGGCTGGGACTTCAGGAACATGTGATTAGGCTGTATTCAAAGCAGATCACCATTGCGATCAACGT CCTCCATGAGCATGGCATAGTCCACCGTGACATTAAAGGTGCCAATATCTTCCTTACCTCATCTGGATTAATCAAACTGGGAGATTTTGGATGTTCAGTA AAGCTCAAAAACAATGCCCAGACCATGCCTGGTGAAGTGAACAGCACCCTGGGGACAGCAGCATACATGGCACCTGAAGTCATCACTCGTGCCAAAGGAG AGGGCCATGGGCGTGCGGCCGACATCTGGAGTCTGGGGTGTGTTGTCATAGAGATGGTGACTGGCAAGAGGCCTTGGCATGAGTATGAGCACAACTTTCA AATTATGTATAAAGTGGGGATGGGACATAAGCCACCAATCCCTGAAAGATTAAGCCCTGAAGGAAAGGACTTCCTTTCTCACTGCCTTGAGAGTGACCCA AAGATGAGATGGACCGCCAGCCAGCTCCTCGACCATTCGTTTGTCAAGGTTTGCACAGATGAAGAATGAAGCCTAGTAGAATATGGACTTGGAAAATTCT CTTAATCACTACTGTATGTAATATTTACATAAAGACTGTGCTGAGAAGCAGTATAAGCCTTTTTAACCTTCCAAGACTGAAGACTGCACAGGTGACAAGC GTCACTTCTCCTGCTGCTCCTGTTTGTCTGATGTGGCAAAAGGCCCTCTGGAGGGCTGGTGGCCACGAGGTTAAAGAAGCTGCATGTTAAGTGCCATTAC TACTGTACACGGACCATCGCCTCTGTCTCCTCCGTGTCTCGCGCGACTGAGAACCGTGACATCAGCGTAGTGTTTTGACCTTTCTAGGTTCAAAAGAAGT TGTAGTGTTATCAGGCGTCCCATACCTTGTTTTTAATCTCCTGTTTGTTGAGTGCACTGACTGTGAAACCTTTACCTTTTTTGTTGTTGTTGGCAAGCTG CAGGTTTGTAATGCAAAAGGCTGATTACTGAAATTTAAGAAAAAGGTTCTTTTTTCAATAAATGGTTTATTTTAGGAAAGCTCA

refseq_MAP3K4.F1 (forward primer)
refseq_MAP3K4.R1 (reverse primer)
Single exon skipping

Frame 0

KMAAARTAPAAG*RRRRSRVTPALRGSGAPRQA AAYCPPRPCGAPCTDERSRCRAGPSSRLCRHACR RHGGAAATAAAATTATGTRDRVRTRVLLGGEAR GHIGRFSLQES*I*SRRLLR*NKYRESLWYLSP QHTSTDETHVNQTSEE*CGEASQSV*FERKNECT KSAST*RHWKNSGECGRIQL*AGEKDPSSS*NN RA*S*KKCTVLIHVRLSGWIFAKKINSRCGSQ* ALPQPWL*QC*ASSICAHAYSQTCTPDF*D*LSS RSFKVF*NFTTFAKAYLSLKEKRQGAKRTRKYV WFLA*PI*RTDLVRATSLACRTDN*RPGLLFIY SPSSHPRYY**NPYFQSRLWELRLC*R*SWF*WY FSRRAVQSHSWNKDCRLLNTS*ASPTPEGLI*A GKTDNGAARVHRSTLSIIAGSSEGL*KICCKRL PGQGAGTLFVVKHHKRLKSEIKDYGHCFGHQEFI RHWLASV*NPFPSTIQR**AGV*G**HRRRIKG VGK*YG*E*RRTNL*S*GTGNQTAHR*QLRHPV AGLHIQEA*EARI*G*FSWLGSTRLEHRSRL**T LSDFYL*TICRQSTEADGVKKVNFKTSQANGWF LAKGTYSIGKERSSSGVF*ISRSHVGFRLCAVV KDTTFI*GEMQCCVVGGAEGHGFTFIRTCLPSSL PSPSECHT*VSEVKIGAETCWRTISLEY*AAGE RV*GGPEGRPADEAVLPVHAAGGSGGLGEARLQ H*RF*RGST*NAYGVF*LHEKLDPNATAITSSIA *FKKSVRRRMEFHQRNNSLHTGRRSTGREAFL* HCRNAAEIYRKFFRIWLTGELC*ILD*CG*QQC FRRNQEVCYRDQSSPEGALP*SQRKGFQSTWIC* NVEKGPGNSSRIQAFSPS*RPPGCSEIKTVCQG ANSWVRKLANVCSRHSC*GEEYYFAVTQCSCRK GLFKRFR*RTHRCLSASDQAR*SSP*FRGQLGHL GGTACQSRASGGDC*HPEKHAGG*SFTSCHAVC ASHNSEKSFPAVH*GTYDSVPGADIQSAGHRQS FAAAEE*CIGAMQQDKQCH*PRGPHVHIRI*C*G **I*ICHLATVLPRSNDSGVQFWI*VS*RSCSF DVWGV*TEDRRQIYKLCPEVDELCPD*M*EW*R YKTQVGDSRI*FSTSN*TCLYFSFTRR*LLEFTS LDE*MHWPCHRKTTQSCYRFVPCHSSEQPPSYE GTSMP**PS*PTPHYPHSRGIQHSEHAFRRAEP WQPCCCCCCCCCCCCCQSAQPLWW*LCAAQIHQQ CP*YQGFQRS*K*SIGFHSC*IAV*VPESSLKP HGGAR*TSISKRRFKWVHKKKLGTSDTNQPE*R YCF*TRTHRSYPEVSPIV*RKEVPRNEEKEYHWS SL*YA*VL**CYARWLEEGDLQMAKRKQNWRRP VWEGVHLHQRRHRGADGHERDSIST**P*DYQG NCRRIENIRRHQTPQSGSVFWCGAP*RRNVHLHG VLR*GDFRRGVKAGTSGTCD*AVFKADHHCDQR PP*AWHSPP*H*RCQYLPYLIWINQTGRFWMFS KAQKQCPDHAW*SEQHPGDSSIHGT*SHHSCQRR GPWACGRHLESGVCCHRDGDWQEALA*V*AQLS NYV*SGDGT*ATNP*KIKP*RKGLPFSLP*E*P KDEMDRQPAPRPFVCQGLHR*RMKPSRIWTWKIL LITTVCNIYIKTVLRSSISLFNLPRLKTAQVTS VTSPAAPVCLMWQKALWRAGGHEVKEAAC*VPL LLYTDHRLCLLRVSRD*EP*HQRSVLTFLGSKEV VVLSGVPYLVFNLLFVECTDCETFTFFVVVGKL QVCNAKG*LLKFKKKVLFSINGLF*ESS

Frame 1

RWPRRARLLRRGRGGGGVESLPHFGAPVPRARL QLTARRGHAGLRARMREAAAALVPPPAFAVTPA AAMEEPPPPPPPPPPPPEPETESEPECCLAARQE GTLGDSACKSPESDLEDFSDETNTENLYGTSPP STPRQMKRMSTKHQRNNVGRPASRSNLKEKMNA PNQPPHKDTGKTVENVEEYSYKQEKKIRAALRTT ERDRKKNVQCSFMLDSVGGSLPKKSIPDVDLNK PYLSLGCSNAKLPVSVPMPIARPARQTSRTDCP ADRLKFFETLRLLLKLTSVSKKKDREQRGQENTS GFWLNRSNELIWLELQAWHAGRTINDQDFFLYT ARQAIPDIINEILTFKVDYGSFAFVRDRAGFNG TSVEGQCKATPGTKIVGYSTHHEHLQRQRVSFEQ VKRIMELLEYIEALYPSLQALQKDYEKYAAKDF QDRVQALCLWLNITKDLNQKLRIMGTVLGIKNL SDIGWPVFEIPSPRPSKGNEPEYEGDDTEGELKE LESSTDESEEEQISDPRVPEIRQPIDNSFDIQS RDCISKKLERLESEDDSLGWGAPDWSTEAGFSR HCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGS LQRARIALVKNDRPVEFSEFPDPMWGSDYVQLS RTPPSSEEKCSAVSWEELKAMDLPSFEPAFLVL CRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVR ECKEVLKGGLLMKQYYQFMLQEVLEDLEKPDCN IDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQAS HSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCD IAGMLLKSTGSFLEFGLQESCAEFWTSADDSSA SDEIRRSVIEISRALKELFHEARERASKALGFA KMLRKDLEIAAEFRLSAPVRDLLDVLKSKQYVKV QIPGLENLQMFVPDTLAEEKSIILQLLNAAAGK DCSKDSDDVLIDAYLLLTKHGDRARDSEDSWGT WEAQPVKVVPQVETVDTLRSMQVDNLLLVVMQSA HLTIQRKAFQQSIEGLMTLCQEQTSSQPVIAKA LQQLKNDALELCNRISNAIDRVDHMFTSEFDAE VDESESVTLQQYYREAMIQGYNFGFEYHKEVVRL MSGEFRQKIGDKYISFARKWMNYVLTKCESGRG TRPRWATQGFDFLQAIEPAFISALPEDDFLSLQ ALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPMK VPRCHSDPPNPHLIIPTPEGFSTRSMPSDARSH GSPAAAAAAAAAAVAASRPSPSGGDSVLPKSIS SAHDTRGSSVPENDRLASIAAELQFRSLSRHSSP TEERDEPAYPRGDSSGSTRRSWELRTLISQSKD TASKLGPIEAIQKSVRLFEEKRYREMRRKNIIG QVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQ YGKVYTCISVDTGELMAMKEIRFQPNDHKTIKE TADELKIFEGIKHPNLVRYFGVELHREEMYIFM EYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINV LHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSV KLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKG EGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQ IMYKVGMGHKPPIPERLSPEGKDFLSHCLESDP KMRWTASQLLDHSFVKVCTDEE*SLVEYGLGKF S*SLLYVIFT*RLC*EAV*AFLTFQD*RLHR*QA SLLLLLLFV*CGKRPSGGLVATRLKKLHVKCHY YCTRTIASVSSVSRATENRDISVVF*PF*VQKK L*CYQASHTLFLISCLLSALTVKPLPFLLLLASC RFVMQKADY*NLRKRFFFQ*MVYFRKA

Frame 2

DGRGAHGSCGGVEAEAESSHSRTSGLRCPAPGC SLLPAAAMRGSVHG*EKPLPRWSLLPPLPSRLP PPWRSRRHRRRRHHRHRNPRPSQNPSAAWRRGK RAHWEIQLARVLNLI*KTSPMKQIQRIFMVPLPP AHLDR*NACQPNIRGIMWGGQPVGLI*KKK*MH QISLHIKTLEKQWRMWKNTAISRRKRSEQLLEQ QSVIVKKMYSAHSC*TQWVDLCQKNQFQMWISIS LTSALAVAMLSFQYLCPCL*PDLHARLLGLTVQ QIV*SFLKLYDFC*SLPQSQRKKTGSKEDKKIR LVSGLTDLTN*SG*SYKPGMQDGQLTTRTSFYIQ PVKPSQILLMKSLLSKSTMGASPLLEIELVLMV LQ*KGSAKPLLEQRL*VTQHIMSISNARGSHLS R*NG*WSC*ST*KHFIHHCRLFRRTMKNMLQKTS RTGCRHSVCG*TSQKT*IRN*GLWALFWASRIY QTLAGQCLKSLPLDHPKVMSRSMRVMTQKEN*R SWKVVRMRVKKNKSLILGYRKSDSP*ITASTSSR GTAYPRSLRGSNLRMILLAGEHQTGAQKQALVD IV*LLFIDHL*TKH*SRWG*ES*F*DFTS*WMV PCKGHV*HW*RTIVQWSFLNFQIPCGVQIMCSCQ GHHLHLRRNAVLCRGRS*RPWIYLHSNLPS*FS AESF*MSYMSV*S*DWSRDLLENHLS*VLSSW* ESVRRS*RAAC**SSTTSSCCRRFWRTWRSPTAT LTLLKRIYIKCLWCILIT*EAGSKCYSNYLKHR IV*KIC*KKNGISPKK*LITYGEEKHRPGSFSV TLQECC*NLQEVF*NLAYRRAVLNFGLVRMTAVL PTKSGGLL*RSVEP*RSSSMKPEKGLPKHLDLL KC*ERTWK*QQNSGFQPQLETSWMF*NQNSMSR CKFLG*KTCKCLFQTLLLRRRVLFCSYSMQLQER TVQKIQMTYSSMPICF*PSTVIEPVIQRTAGAP GRHSLSKSCLRWRLLTP*EACRWIIFY*LSCSL RISQFREKLSSSPLRDL*LCARSRHPVSRSSPKL CSS*RMMHWSYATG*AMPLTAWTTCSHQNLMLR LMNLNLSPCNSTTEKQ*FRGTILDLSIIKKLFV *CLGSLDRR*ETNI*ALPGSG*IMS*LNVRVVEV QDPGGRLKDLIFYKQLNLPLFQLYQKMTS*VYK P**MNALAMS*ENHTVLLQVCTLPFIGTAPVL* RYLDAIVTLLTHTSLSPLQRDSALGACLPTRGAM AALLLLLLLLLLLLLPVGPAPLVVTLCCPNPSA VPMIPGVPAFLKMIDWLP*LLNCSLGP*VVTQA PRRSEMNQHIQEEIQVGPQEEVGNFGH*SARVKI LLLN*DP*KLSRSQSDCLKKRGTEK*GERISLV KFVIRLSPMIMLCTLA*GR*PSNGKEETKLEKA SMGRCTPASASTPGS*WP*KRFDFNLMTIRLSRK LQTN*KYSKASNTPIWFGILVWSSIEKKCTSSW STAMRGL*KRCQGWDFRNM*LGCIQSRSPLRST SSMSMA*STVTLKVPISSLPHLD*SNWEILDVQ* SSKTMPRPCLVK*TAPWGQQHTWHLKSSLVPKE RAMGVRPTSGVWGVLS*RW*LARGLGMSMSTTF KLCIKWGWDISHQSLKD*ALKERTSFLTALRVTQ R*DGPPASSSTIRLSRFAQMKNEA**NMDLENS LNHYCM*YLHKDCAEKQYKPF*PSKTEDCTGDK RHFSCCSCLSDVAKGPLEGWWPRG*RSCMLSAIT TVHGPSPLSPPCLARLRTVTSA*CFDLSRFKRS CSVIRRPIPCF*SPVC*VH*L*NLYLFCCCWQA AGL*CKRLITEI*EKGSFFNKWFILGKL