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Sequence NM_198080
CCCGGAGCCGGGGAGGGAGGGAGCGAGGTTCGGACACCGGCGGCGGCTGCCTGGCCTTTCCATGAGCCCGCGGCGGACCCTCCCGCGCCCCCTCTCGCTCTGCCTCTCCCTCTGCCTCTGCCTCTGCCTGGCCGCGGCTCTGGGAAGTGCGCAGTCCGGGTCGTGTAGGGATAAAAAGAACTGTAAGGTGGTCTTTTCCC
AGCAGGAACTGAGGAAGCGGCTAACACCCCTGCAGTACCATGTCACTCAGGAGAAAGGGACCGAAAGTGCCTTTGAAGGAGAATACACACATCACAAAGA
TCCTGGAATATATAAATGTGTTGTTTGTGGAACTCCATTGTTTAAGTCAGAAACCAAATTTGACTCCGGTTCAGGTTGGCCTTCATTCCACGATGTGATC
AATTCTGAGGCAATCACATTCACAGATGACTTTTCCTATGGGATGCACAGGGTGGAAACAAGCTGCTCTCAGTGTGGTGCTCACCTTGGGCACATTTTTG
ATGATGGGCCTCGTCCAACTGGGAAAAGATACTGCATAAATTCGGCTGCCTTGTCTTTTACACCTGCGGATAGCAGTGGCACCGCCGAGGGAGGCAGTGG
GGTCGCCAGCCCGGCCCAGGCAGACAAAGCGGAGCTCTAGAGTAATGGAGAGTGATGGAAACAAAGTGTACTTAATGCACAGCTTATTAAAAAAATCAAA
ATTGTTATCTTAATAGATATATTTTTTCAAAAACTATAAGGGCAGTTTTGTGCTATTGATATTTTTTCTTCTTTTGCTTAAACAGAAGCCCTGGCCATCC
ATGTATTTTGCAATTGACTAGATCAAGAACTGTTTATAGCTTTAGCAAATGGAGACAGCTTTGTGAAACTTCTTCACAAGCCACTTATACCCTTTGGCAT
TCTTTTCTTTGAGCACATGGCTTCTTTTGCAGTTTTTCCCCCTTTGATTCAGAAGCAGAGGGTTCATGGTCTTCAAACATGAAAATAGAGATCTCCTCTG
CAGTGTAGAGACCAGAGCTGGGCAGTGCAGGGCATGGAGACCTGCAAGACACATGGCCTTGAGGCCTTTGCACAGACCCACCTAAGATAAGGTTGGAGTG
ATGTTTTAATGAGACTGTTCAGCTTTGTGGAAAGTTTGAGCTAAGGTCATTTTTTTTTTTCTCACTGAAAGGGTGTGAAGGTCTAAAGTCTTTCCTTATG
TTAAATTGTTGCCAGATCCAAAGGGGCATACTGAGTGTTGTGGCAGAGAAGTAAACATTACCACACTGTTAGGCCTTTATTTTATTTTATTTTCCATCGA
AAGCATTGGAGGCCCAGTGCAATGGCTCACGCCTGTGATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCACGAGGTCAGGAGATGGAGACCATCCTG
GCTAACATGGTGAAACCCCGTCTCTACTAAAAATACGAAAAATTAGCCAGGCGTGGTGGTGGGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGG
AGAATGGCGTGAACCCGGAAGGCGGAGCTTGCAGTTAGCCGAGATCATGCCACTGCACTCCAGCCTACATGACAATGTGACACTCCATCTCAAAAAATAA
TAATAATAACAATATAAGAACTAGCTGGGCATGGTGGCGCATGCATGTAGTCCCAGCTACTCCTGAGGCTCAGTCAGGAGAATCGCTTGAACTTGGGAGG
CGGAGGTTGCAGTGAGCTGAGCTCATACCACTGCACTCCAGCCTGAACAGAGTGAGATCCTGTCAAAAAAGAAAAGAAAAAGAAAGCAGCATTCAAATGT
AAGACAACTGTAAAATATTGAGCCCCACTTGGTCTAAAATTCAAAAAGAAGAACGCCTGTCCATCGCCTTTTTATAAGTCCTTCTCTCCACACCTAAAAG
CAGCTGCAGCTGGAAGGGCACAAATTCCACTGTGTAAAATAAAATATTAGGGGCAACACACTTCATCAAGGCAGCAGGAATGAGAGAGAGCAGAGAAGAT
CAAGGATGAAGTCTTGGGTACTGAAAAATTCAGTGCTGGGCAGAAAAACTGACAGGGCAGTACAAGTAACAAACAGAATCCAAGTGGGGTGGCCCTTGTG
CACAGAGCTCCAGGTGACCTCTGGAGAGACATGGGCATTCACATGGAAAGCTAAAACGGAAGCTCAAGTTTCATACTCAACATAATCTTCTGTGTGACAA
AGGACAAGCCATGTAGCCTCTCTGTGCCTATTTCTTCATGCATAAACTGGGACTCATAATATTTGTAAAATGTATTGATACTCTCAGGGCAAATTCACTA
TATTGCTATACAGTTGAGATCAGTGTTGTAAAATTAAACTGATCTGGTTCTAATTGCCTCAAAGGCCAAAGCCCAGGCATTTGAAATGGAAAGAAGCAGA
GAGGAGGCTGACTTAGCTGATTGGTATGGAAACAGTTGGGCCAAGAGCCAGAATTTCCCTTTGTAGCAACACGGCTAGTTTTACTTTGAGAAGCTCTGCT
CAGCTGCTTTATAACATTAAGTCTGGCGGAATGGATGTCACTGTGCACAATAAAGTTTTCACAAGTATAAACAATGGTGATGTAAGTCAACATTGCTGTA
GCCAGGTGTGAAGGTTGTATGGTGTGTGACGAATGTACATCATGTTTGTAGGTTTGGATGCTAATCTTGAATTGTAGTTTAAAAAATACGTATTTTTGTA
ACTCTTTGAAAGTTTATGAAGACTGACAGCTTTCCTTGTAAGCACTAAGAGAAAAAAAAGAAAGAGGGACATTTGACAATTTTAAAGAAACAACAAGAAA
TTAGAATGAAAATCTGTGACAAACAGCGTCAGTGTGGCCATGTCCACATTCCTACATGTCTCTCTCTACAAGCACCTCTCTAAGAAGCCTGACATCCCGG
TGGACTCTTTATAGTCATGTACACTTGATTCCAGATGAGCTCTGGTCTTATCTGGATGCTCAGATAAGAGGTTTCTATCTGAGCATCCAGATGTTCCCTC
AGGTTCCAAGACATTTCACCCCAGGCCCTGGGTTCACTCTGGAATTCGTAGGCTTCACGTCTCTCTAGAAATGACGTGTAAAATTTAAGACCAGACCTCA
GCCATCAGCGTCCAGACCATCCTAGAAGTCTTTCCCAATCTCACAGAGAAAGCCCTAGTATTTCCCAGTGACCCCAGGATTCCACGTTGGGGTGGCCAAA
GAAATAGGTCTCTCAGGGCTTTGCCACAGCCTCCAGCCCATCCTTCAGAGGCACACACAGCACCTCTCGGCTGCTCCAGCTCTGTAGGATAGCCTCCCCT
GGGGTCCGTGGGACGCGGGCCACAGTGTTGAGGTAGACAAGGAGGATCAGTGAGAGGCCTCTTCCCTCTCCACAGAGACTGGATTGTCATTGTTCCTTCA
TTTATATCGTAGGGCTTAACATTTCACTCAAAAAAAAGCCCCTCTTTTTCTAATCCTTAGTCTTTGTTTCAAGGAAAGCCAGTTTTTCTTCTACCACATT
TTCCAGGATCGACTTTAAGAAAAATGCAACATCTATTGAAAAAAAGTGGGGTGTATGCATGTGGTTTAATTCCAGATTGCTTTTGGGTTTAAGTGGTATC
AAATTTCAGTATATTTCTGTCTTATGTGAAAGAAATATATTACTAAAACGTCAGTGAGCAATAATGTCAGCTGTCAAGCACTAGATTTATTTTTGCAGGA
TATGGAGTGCAATGAACTGAGTCAATATGGCAAGGTGTATGTGATCTGTGGGAGTTATGCCATTTAACATAGGAAGTGCATGGGACTTTCCCTCTCTGCA
CTCCAGCTCTTACTGTACCATTAGAAGATGCAGAATTCTGTTGGTGTGCAAAAAGTATAGCCTTACATTCAAGCAGAATGGATCTGAAGAAAGCAGCAAT
ATCTGTTACTAGAGAACATTCCCATGTGTTTAAACTCTTCACTTCTTAGATGCATTTAAATTCTTAATGCAAATGACGTAGCAATTTGAAAACTTCTCCG
TATTACTTGTGTTTAAAATGTCTTGCTTTAAATACAAAACAAATGGTAAAGGGGATTATCTTTTGTTTAGATGGTTAAATATTATTTTTGCCTTAGATAG
CTTTGTAATAATTTTTCTCCAGACAGTTCAACACTTTTGAAAAATGACATGAATTTTCATTAAAAACCCTTTTCCTATGTTTATTGTATACAAGAATTAT
GCAATAAAATTTCTTTATAAAAATA
- refseq_MSRB3.F1 (forward primer)
- refseq_MSRB3.R1 (reverse primer)
- Single exon skipping
Frame 0
PGAGEGGSEVRTPAAAAWPFHEPAADPPAPPLALPLPLPLPLPGRGSGKCAVRVV*G*KEL*GGLFP
AGTEEAANTPAVPCHSGERDRKCL*RRIHTSQR
SWNI*MCCLWNSIV*VRNQI*LRFRLAFIPRCD
QF*GNHIHR*LFLWDAQGGNKLLSVWCSPWAHF*
*WASSNWEKILHKFGCLVFYTCG*QWHRRGRQW
GRQPGPGRQSGALE*WRVMETKCT*CTAY*KNQ
NCYLNRYIFSKTIRAVLCY*YFFFFCLNRSPGHP
CILQLTRSRTVYSFSKWRQLCETSSQATYTLWH
SFL*AHGFFCSFSPFDSEAEGSWSSNMKIEISS
AV*RPELGSAGHGDLQDTWP*GLCTDPPKIRLE*
CFNETVQLCGKFELRSFFFFSLKGCEGLKSFLM
LNCCQIQRGILSVVAEK*TLPHC*AFILFYFPS
KALEAQCNGSRL*SQHFGRPRRVDHEVRRWRPSW
LTW*NPVSTKNTKN*PGVVVGTCSPSYSGG*GR
RMA*TRKAELAVSRDHATALQPT*QCDTPSQKI
IIITI*ELAGHGGACM*SQLLLRLSQENRLNLGG
GGCSELSSYHCTPA*TE*DPVKKEKKKKAAFKC
KTTVKY*APLGLKFKKKNACPSPFYKSFSPHLK
AAAAGRAQIPLCKIKY*GQHTSSRQQE*ERAEKI
KDEVLGTEKFSAGQKN*QGSTSNKQNPSGVALV
HRAPGDLWRDMGIHMES*NGSSSFILNIIFCVT
KDKPCSLSVPISSCINWDS*YL*NVLILSGQIHY
IAIQLRSVL*N*TDLVLIASKAKAQAFEMERSR
EEADLADWYGNSWAKSQNFPL*QHG*FYFEKLC
SAAL*H*VWRNGCHCAQ*SFHKYKQW*CKSTLL*
PGVKVVWCVTNVHHVCRFGC*S*IVV*KIRIFV
TL*KFMKTDSFPCKH*EKKKKEGHLTILKKQQE
IRMKICDKQRQCGHVHIPTCLSLQAPL*EA*HPG
GLFIVMYT*FQMSSGLIWMLR*EVSI*ASRCSL
RFQDISPQALGSLWNS*ASRLSRNDV*NLRPDL
SHQRPDHPRSLSQSHRESPSISQ*PQDSTLGWPK
K*VSQGFATASSPSFRGTHSTSRLLQLCRIASP
GVRGTRATVLR*TRRISERPLPSPQRLDCHCSF
IYIVGLNISLKKKPLFF*SLVFVSRKASFSSTTF
SRIDFKKNATSIEKKWGVCMWFNSRLLLGLSGI
KFQYISVLCERNILLKRQ*AIMSAVKH*IYFCR
IWSAMN*VNMARCM*SVGVMPFNIGSAWDFPSLH
SSSYCTIRRCRILLVCKKYSLTFKQNGSEESSN
ICY*RTFPCV*TLHFLDAFKFLMQMT*QFENFS
VLLVFKMSCFKYKTNGKGDYLLFRWLNIIFALDS
FVIIFLQTVQHF*KMT*IFIKNPFPMFIVYKNY
AIKFLYKN
Frame 1
PEPGREGARFGHRRRLPGLSMSPRRTLPRPLSLCLSLCLCLCLAAALGSAQSGSCRDKKNCKVVFS
QQELRKRLTPLQYHVTQEKGTESAFEGEYTHHKD
PGIYKCVVCGTPLFKSETKFDSGSGWPSFHDVI
NSEAITFTDDFSYGMHRVETSCSQCGAHLGHIF
DDGPRPTGKRYCINSAALSFTPADSSGTAEGGSG
VASPAQADKAEL*SNGE*WKQSVLNAQLIKKIK
IVILIDIFFQKL*GQFCAIDIFSSFA*TEALAI
HVFCN*LDQELFIALANGDSFVKLLHKPLIPFGI
LFFEHMASFAVFPPLIQKQRVHGLQT*K*RSPL
QCRDQSWAVQGMETCKTHGLEAFAQTHLR*GWS
DVLMRLFSFVESLS*GHFFFSH*KGVKV*SLSLC
*IVARSKGAY*VLWQRSKHYHTVRPLFYFIFHR
KHWRPSAMAHACDPSTLGGQGGWITRSGDGDHP
G*HGETPSLLKIRKISQAWWWAPVVPATQEAEAG
EWREPGRRSLQLAEIMPLHSSLHDNVTLHLKK*
***QYKN*LGMVAHACSPSYS*GSVRRIA*TWE
AEVAVS*AHTTALQPEQSEILSKKKRKRKQHSNV
RQL*NIEPHLV*NSKRRTPVHRLFISPSLHT*K
QLQLEGHKFHCVK*NIRGNTLHQGSRNEREQRR
SRMKSWVLKNSVLGRKTDRAVQVTNRIQVGWPLC
TELQVTSGETWAFTWKAKTEAQVSYST*SSV*Q
RTSHVASLCLFLHA*TGTHNICKMY*YSQGKFT
ILLYS*DQCCKIKLIWF*LPQRPKPRHLKWKEAE
RRLT*LIGMETVGPRARISLCSNTASFTLRSSA
QLLYNIKSGGMDVTVHNKVFTSINNGDVSQHCC
SQV*RLYGV*RMYIMFVGLDANLEL*FKKYVFL*
LFESL*RLTAFLVSTKRKKRKRDI*QF*RNNKK
LE*KSVTNSVSVAMSTFLHVSLYKHLSKKPDIP
VDSL*SCTLDSR*ALVLSGCSDKRFLSEHPDVPS
GSKTFHPRPWVHSGIRRLHVSLEMTCKI*DQTS
AISVQTILEVFPNLTEKALVFPSDPRIPRWGGQ
RNRSLRALPQPPAHPSEAHTAPLGCSSSVG*PPL
GSVGRGPQC*GRQGGSVRGLFPLHRDWIVIVPS
FIS*GLTFHSKKSPSFSNP*SLFQGKPVFLLPH
FPGSTLRKMQHLLKKSGVYACGLIPDCFWV*VVS
NFSIFLSYVKEIYY*NVSEQ*CQLSSTRFIFAG
YGVQ*TESIWQGVCDLWELCHLT*EVHGTFPLC
TPALTVPLEDAEFCWCAKSIALHSSRMDLKKAAI
SVTREHSHVFKLFTS*MHLNS*CK*RSNLKTSP
YYLCLKCLALNTKQMVKGIIFCLDG*ILFLP*I
AL**FFSRQFNTFEK*HEFSLKTLFLCLLYTRIM
Q*NFFIKI
Frame 2
RSRGGRERGSDTGGGCLAFP*ARGGPSRAPSRSASPSASASAWPRLWEVRSPGRVGIKRTVRWSFP
SRN*GSG*HPCSTMSLRRKGPKVPLKENTHITK
ILEYINVLFVELHCLSQKPNLTPVQVGLHSTM*S
ILRQSHSQMTFPMGCTGWKQAALSVVLTLGTFL
MMGLVQLGKDTA*IRLPCLLHLRIAVAPPREAV
GSPARPRQTKRSSRVMESDGNKVYLMHSLLKKSK
LLS**IYFFKNYKGSFVLLIFFLLLLKQKPWPS
MYFAID*IKNCL*L*QMETAL*NFFTSHLYPLA
FFSLSTWLLLQFFPL*FRSRGFMVFKHENRDLLC
SVETRAGQCRAWRPARHMALRPLHRPT*DKVGV
MF**DCSALWKV*AKVIFFFLTERV*RSKVFPY
VKLLPDPKGHTECCGREVNITTLLGLYFILFSIE
SIGGPVQWLTPVIPALWEAKAGGSRGQEMETIL
ANMVKPRLY*KYEKLARRGGGHL*SQLLRRLRQ
ENGVNPEGGACS*PRSCHCTPAYMTM*HSISKNN
NNNNIRTSWAWWRMHVVPATPEAQSGESLELGR
RRLQ*AELIPLHSSLNRVRSCQKRKEKESSIQM
*DNCKILSPTWSKIQKEERLSIAFL*VLLSTPKS
SCSWKGTNSTV*NKILGATHFIKAAGMRESRED
QG*SLGY*KIQCWAEKLTGQYK*QTESKWGGPC
AQSSR*PLERHGHSHGKLKRKLKFHTQHNLLCDK
GQAM*PLCAYFFMHKLGLIIFVKCIDTLRANSL
YCYTVEISVVKLN*SGSNCLKGQSPGI*NGKKQ
RGG*LS*LVWKQLGQEPEFPFVATRLVLL*EALL
SCFITLSLAEWMSLCTIKFSQV*TMVM*VNIAV
ARCEGCMVCDECTSCL*VWMLILNCSLKNTYFC
NSLKVYED*QLSL*ALREKKERGTFDNFKETTRN
*NENL*QTASVWPCPHSYMSLSTSTSLRSLTSR
WTLYSHVHLIPDELWSYLDAQIRGFYLSIQMFP
QVPRHFTPGPGFTLEFVGFTSL*K*RVKFKTRPQ
PSASRPS*KSFPISQRKP*YFPVTPGFHVGVAK
EIGLSGLCHSLQPILQRHTQHLSAAPAL*DSLP
WGPWDAGHSVEVDKEDQ*EASSLSTETGLSLFLH
LYRRA*HFTQKKAPLFLILSLCFKESQFFFYHI
FQDRL*EKCNIY*KKVGCMHVV*FQIAFGFKWY
QISVYFCLM*KKYITKTSVSNNVSCQALDLFLQD
MECNELSQYGKVYVICGSYAI*HRKCMGLSLSA
LQLLLYH*KMQNSVGVQKV*PYIQAEWI*RKQQ
YLLLENIPMCLNSSLLRCI*ILNANDVAI*KLLR
ITCV*NVLL*IQNKW*RGLSFV*MVKYYFCLR*
LCNNFSPDSSTLLKNDMNFH*KPFSYVYCIQEL
CNKISL*K
Sequence NM_001031679
CCCGGAGCCGGGGAGGGAGGGAGCGAGGTTCGGACACCGGCGGCGGCTGCCTGGCCTTTCCATGAGCCCGCGGCGGACCCTCCCGCGCCCCCTCTCGCTCTGCCTCTCCCTCTGCCTCTGCCTCTGCCTGGCCGCGGCTCTGGGAAGTGCGCAGTCCGCTCTTGCCCCTGTTCTTTGCTTCTCGTTTTGTTGGTGAAGAT
ATCACAGTGATGTCTGCATTCAACCTGCTGCATTTGGTGACAAAGAGCCAGCCAGTAGCCCTTCGAGCCTGTGGGCTTCCCTCAGGGTCGTGTAGGGATA
AAAAGAACTGTAAGGTGGTCTTTTCCCAGCAGGAACTGAGGAAGCGGCTAACACCCCTGCAGTACCATGTCACTCAGGAGAAAGGGACCGAAAGTGCCTT
TGAAGGAGAATACACACATCACAAAGATCCTGGAATATATAAATGTGTTGTTTGTGGAACTCCATTGTTTAAGTCAGAAACCAAATTTGACTCCGGTTCA
GGTTGGCCTTCATTCCACGATGTGATCAATTCTGAGGCAATCACATTCACAGATGACTTTTCCTATGGGATGCACAGGGTGGAAACAAGCTGCTCTCAGT
GTGGTGCTCACCTTGGGCACATTTTTGATGATGGGCCTCGTCCAACTGGGAAAAGATACTGCATAAATTCGGCTGCCTTGTCTTTTACACCTGCGGATAG
CAGTGGCACCGCCGAGGGAGGCAGTGGGGTCGCCAGCCCGGCCCAGGCAGACAAAGCGGAGCTCTAGAGTAATGGAGAGTGATGGAAACAAAGTGTACTT
AATGCACAGCTTATTAAAAAAATCAAAATTGTTATCTTAATAGATATATTTTTTCAAAAACTATAAGGGCAGTTTTGTGCTATTGATATTTTTTCTTCTT
TTGCTTAAACAGAAGCCCTGGCCATCCATGTATTTTGCAATTGACTAGATCAAGAACTGTTTATAGCTTTAGCAAATGGAGACAGCTTTGTGAAACTTCT
TCACAAGCCACTTATACCCTTTGGCATTCTTTTCTTTGAGCACATGGCTTCTTTTGCAGTTTTTCCCCCTTTGATTCAGAAGCAGAGGGTTCATGGTCTT
CAAACATGAAAATAGAGATCTCCTCTGCAGTGTAGAGACCAGAGCTGGGCAGTGCAGGGCATGGAGACCTGCAAGACACATGGCCTTGAGGCCTTTGCAC
AGACCCACCTAAGATAAGGTTGGAGTGATGTTTTAATGAGACTGTTCAGCTTTGTGGAAAGTTTGAGCTAAGGTCATTTTTTTTTTTCTCACTGAAAGGG
TGTGAAGGTCTAAAGTCTTTCCTTATGTTAAATTGTTGCCAGATCCAAAGGGGCATACTGAGTGTTGTGGCAGAGAAGTAAACATTACCACACTGTTAGG
CCTTTATTTTATTTTATTTTCCATCGAAAGCATTGGAGGCCCAGTGCAATGGCTCACGCCTGTGATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCA
CGAGGTCAGGAGATGGAGACCATCCTGGCTAACATGGTGAAACCCCGTCTCTACTAAAAATACGAAAAATTAGCCAGGCGTGGTGGTGGGCACCTGTAGT
CCCAGCTACTCAGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGAAGGCGGAGCTTGCAGTTAGCCGAGATCATGCCACTGCACTCCAGCCTACATGAC
AATGTGACACTCCATCTCAAAAAATAATAATAATAACAATATAAGAACTAGCTGGGCATGGTGGCGCATGCATGTAGTCCCAGCTACTCCTGAGGCTCAG
TCAGGAGAATCGCTTGAACTTGGGAGGCGGAGGTTGCAGTGAGCTGAGCTCATACCACTGCACTCCAGCCTGAACAGAGTGAGATCCTGTCAAAAAAGAA
AAGAAAAAGAAAGCAGCATTCAAATGTAAGACAACTGTAAAATATTGAGCCCCACTTGGTCTAAAATTCAAAAAGAAGAACGCCTGTCCATCGCCTTTTT
ATAAGTCCTTCTCTCCACACCTAAAAGCAGCTGCAGCTGGAAGGGCACAAATTCCACTGTGTAAAATAAAATATTAGGGGCAACACACTTCATCAAGGCA
GCAGGAATGAGAGAGAGCAGAGAAGATCAAGGATGAAGTCTTGGGTACTGAAAAATTCAGTGCTGGGCAGAAAAACTGACAGGGCAGTACAAGTAACAAA
CAGAATCCAAGTGGGGTGGCCCTTGTGCACAGAGCTCCAGGTGACCTCTGGAGAGACATGGGCATTCACATGGAAAGCTAAAACGGAAGCTCAAGTTTCA
TACTCAACATAATCTTCTGTGTGACAAAGGACAAGCCATGTAGCCTCTCTGTGCCTATTTCTTCATGCATAAACTGGGACTCATAATATTTGTAAAATGT
ATTGATACTCTCAGGGCAAATTCACTATATTGCTATACAGTTGAGATCAGTGTTGTAAAATTAAACTGATCTGGTTCTAATTGCCTCAAAGGCCAAAGCC
CAGGCATTTGAAATGGAAAGAAGCAGAGAGGAGGCTGACTTAGCTGATTGGTATGGAAACAGTTGGGCCAAGAGCCAGAATTTCCCTTTGTAGCAACACG
GCTAGTTTTACTTTGAGAAGCTCTGCTCAGCTGCTTTATAACATTAAGTCTGGCGGAATGGATGTCACTGTGCACAATAAAGTTTTCACAAGTATAAACA
ATGGTGATGTAAGTCAACATTGCTGTAGCCAGGTGTGAAGGTTGTATGGTGTGTGACGAATGTACATCATGTTTGTAGGTTTGGATGCTAATCTTGAATT
GTAGTTTAAAAAATACGTATTTTTGTAACTCTTTGAAAGTTTATGAAGACTGACAGCTTTCCTTGTAAGCACTAAGAGAAAAAAAAGAAAGAGGGACATT
TGACAATTTTAAAGAAACAACAAGAAATTAGAATGAAAATCTGTGACAAACAGCGTCAGTGTGGCCATGTCCACATTCCTACATGTCTCTCTCTACAAGC
ACCTCTCTAAGAAGCCTGACATCCCGGTGGACTCTTTATAGTCATGTACACTTGATTCCAGATGAGCTCTGGTCTTATCTGGATGCTCAGATAAGAGGTT
TCTATCTGAGCATCCAGATGTTCCCTCAGGTTCCAAGACATTTCACCCCAGGCCCTGGGTTCACTCTGGAATTCGTAGGCTTCACGTCTCTCTAGAAATG
ACGTGTAAAATTTAAGACCAGACCTCAGCCATCAGCGTCCAGACCATCCTAGAAGTCTTTCCCAATCTCACAGAGAAAGCCCTAGTATTTCCCAGTGACC
CCAGGATTCCACGTTGGGGTGGCCAAAGAAATAGGTCTCTCAGGGCTTTGCCACAGCCTCCAGCCCATCCTTCAGAGGCACACACAGCACCTCTCGGCTG
CTCCAGCTCTGTAGGATAGCCTCCCCTGGGGTCCGTGGGACGCGGGCCACAGTGTTGAGGTAGACAAGGAGGATCAGTGAGAGGCCTCTTCCCTCTCCAC
AGAGACTGGATTGTCATTGTTCCTTCATTTATATCGTAGGGCTTAACATTTCACTCAAAAAAAAGCCCCTCTTTTTCTAATCCTTAGTCTTTGTTTCAAG
GAAAGCCAGTTTTTCTTCTACCACATTTTCCAGGATCGACTTTAAGAAAAATGCAACATCTATTGAAAAAAAGTGGGGTGTATGCATGTGGTTTAATTCC
AGATTGCTTTTGGGTTTAAGTGGTATCAAATTTCAGTATATTTCTGTCTTATGTGAAAGAAATATATTACTAAAACGTCAGTGAGCAATAATGTCAGCTG
TCAAGCACTAGATTTATTTTTGCAGGATATGGAGTGCAATGAACTGAGTCAATATGGCAAGGTGTATGTGATCTGTGGGAGTTATGCCATTTAACATAGG
AAGTGCATGGGACTTTCCCTCTCTGCACTCCAGCTCTTACTGTACCATTAGAAGATGCAGAATTCTGTTGGTGTGCAAAAAGTATAGCCTTACATTCAAG
CAGAATGGATCTGAAGAAAGCAGCAATATCTGTTACTAGAGAACATTCCCATGTGTTTAAACTCTTCACTTCTTAGATGCATTTAAATTCTTAATGCAAA
TGACGTAGCAATTTGAAAACTTCTCCGTATTACTTGTGTTTAAAATGTCTTGCTTTAAATACAAAACAAATGGTAAAGGGGATTATCTTTTGTTTAGATG
GTTAAATATTATTTTTGCCTTAGATAGCTTTGTAATAATTTTTCTCCAGACAGTTCAACACTTTTGAAAAATGACATGAATTTTCATTAAAAACCCTTTT
CCTATGTTTATTGTATACAAGAATTATGCAATAAAATTTCTTTATAAAAATA
- refseq_MSRB3.F1 (forward primer)
- refseq_MSRB3.R1 (reverse primer)
- Single exon skipping
Frame 0
PGAGEGGSEVRTPAAAAWPFHEPAADPPAPPLALPLPLPLPLPGRGSGKCAVRSCPCSLLLVLLVKI
SQ*CLHSTCCIW*QRASQ*PFEPVGFPQGRVGI
KRTVRWSFPSRN*GSG*HPCSTMSLRRKGPKVP
LKENTHITKILEYINVLFVELHCLSQKPNLTPVQ
VGLHSTM*SILRQSHSQMTFPMGCTGWKQAALS
VVLTLGTFLMMGLVQLGKDTA*IRLPCLLHLRI
AVAPPREAVGSPARPRQTKRSSRVMESDGNKVYL
MHSLLKKSKLLS**IYFFKNYKGSFVLLIFFLL
LLKQKPWPSMYFAID*IKNCL*L*QMETAL*NF
FTSHLYPLAFFSLSTWLLLQFFPL*FRSRGFMVF
KHENRDLLCSVETRAGQCRAWRPARHMALRPLH
RPT*DKVGVMF**DCSALWKV*AKVIFFFLTER
V*RSKVFPYVKLLPDPKGHTECCGREVNITTLLG
LYFILFSIESIGGPVQWLTPVIPALWEAKAGGS
RGQEMETILANMVKPRLY*KYEKLARRGGGHL*
SQLLRRLRQENGVNPEGGACS*PRSCHCTPAYMT
M*HSISKNNNNNNIRTSWAWWRMHVVPATPEAQ
SGESLELGRRRLQ*AELIPLHSSLNRVRSCQKR
KEKESSIQM*DNCKILSPTWSKIQKEERLSIAFL
*VLLSTPKSSCSWKGTNSTV*NKILGATHFIKA
AGMRESREDQG*SLGY*KIQCWAEKLTGQYK*Q
TESKWGGPCAQSSR*PLERHGHSHGKLKRKLKFH
TQHNLLCDKGQAM*PLCAYFFMHKLGLIIFVKC
IDTLRANSLYCYTVEISVVKLN*SGSNCLKGQS
PGI*NGKKQRGG*LS*LVWKQLGQEPEFPFVATR
LVLL*EALLSCFITLSLAEWMSLCTIKFSQV*T
MVM*VNIAVARCEGCMVCDECTSCL*VWMLILN
CSLKNTYFCNSLKVYED*QLSL*ALREKKERGTF
DNFKETTRN*NENL*QTASVWPCPHSYMSLSTS
TSLRSLTSRWTLYSHVHLIPDELWSYLDAQIRG
FYLSIQMFPQVPRHFTPGPGFTLEFVGFTSL*K*
RVKFKTRPQPSASRPS*KSFPISQRKP*YFPVT
PGFHVGVAKEIGLSGLCHSLQPILQRHTQHLSA
APAL*DSLPWGPWDAGHSVEVDKEDQ*EASSLST
ETGLSLFLHLYRRA*HFTQKKAPLFLILSLCFK
ESQFFFYHIFQDRL*EKCNIY*KKVGCMHVV*F
QIAFGFKWYQISVYFCLM*KKYITKTSVSNNVSC
QALDLFLQDMECNELSQYGKVYVICGSYAI*HR
KCMGLSLSALQLLLYH*KMQNSVGVQKV*PYIQ
AEWI*RKQQYLLLENIPMCLNSSLLRCI*ILNAN
DVAI*KLLRITCV*NVLL*IQNKW*RGLSFV*M
VKYYFCLR*LCNNFSPDSSTLLKNDMNFH*KPF
SYVYCIQELCNKISL*K
Frame 1
PEPGREGARFGHRRRLPGLSMSPRRTLPRPLSLCLSLCLCLCLAAALGSAQSALAPVLCFSFCW*R
YHSDVCIQPAAFGDKEPASSPSSLWASLRVV*G*
KEL*GGLFPAGTEEAANTPAVPCHSGERDRKCL
*RRIHTSQRSWNI*MCCLWNSIV*VRNQI*LRF
RLAFIPRCDQF*GNHIHR*LFLWDAQGGNKLLSV
WCSPWAHF**WASSNWEKILHKFGCLVFYTCG*
QWHRRGRQWGRQPGPGRQSGALE*WRVMETKCT
*CTAY*KNQNCYLNRYIFSKTIRAVLCY*YFFFF
CLNRSPGHPCILQLTRSRTVYSFSKWRQLCETS
SQATYTLWHSFL*AHGFFCSFSPFDSEAEGSWS
SNMKIEISSAV*RPELGSAGHGDLQDTWP*GLCT
DPPKIRLE*CFNETVQLCGKFELRSFFFFSLKG
CEGLKSFLMLNCCQIQRGILSVVAEK*TLPHC*
AFILFYFPSKALEAQCNGSRL*SQHFGRPRRVDH
EVRRWRPSWLTW*NPVSTKNTKN*PGVVVGTCS
PSYSGG*GRRMA*TRKAELAVSRDHATALQPT*
QCDTPSQKIIIITI*ELAGHGGACM*SQLLLRLS
QENRLNLGGGGCSELSSYHCTPA*TE*DPVKKE
KKKKAAFKCKTTVKY*APLGLKFKKKNACPSPF
YKSFSPHLKAAAAGRAQIPLCKIKY*GQHTSSRQ
QE*ERAEKIKDEVLGTEKFSAGQKN*QGSTSNK
QNPSGVALVHRAPGDLWRDMGIHMES*NGSSSF
ILNIIFCVTKDKPCSLSVPISSCINWDS*YL*NV
LILSGQIHYIAIQLRSVL*N*TDLVLIASKAKA
QAFEMERSREEADLADWYGNSWAKSQNFPL*QH
G*FYFEKLCSAAL*H*VWRNGCHCAQ*SFHKYKQ
W*CKSTLL*PGVKVVWCVTNVHHVCRFGC*S*I
VV*KIRIFVTL*KFMKTDSFPCKH*EKKKKEGH
LTILKKQQEIRMKICDKQRQCGHVHIPTCLSLQA
PL*EA*HPGGLFIVMYT*FQMSSGLIWMLR*EV
SI*ASRCSLRFQDISPQALGSLWNS*ASRLSRN
DV*NLRPDLSHQRPDHPRSLSQSHRESPSISQ*P
QDSTLGWPKK*VSQGFATASSPSFRGTHSTSRL
LQLCRIASPGVRGTRATVLR*TRRISERPLPSP
QRLDCHCSFIYIVGLNISLKKKPLFF*SLVFVSR
KASFSSTTFSRIDFKKNATSIEKKWGVCMWFNS
RLLLGLSGIKFQYISVLCERNILLKRQ*AIMSA
VKH*IYFCRIWSAMN*VNMARCM*SVGVMPFNIG
SAWDFPSLHSSSYCTIRRCRILLVCKKYSLTFK
QNGSEESSNICY*RTFPCV*TLHFLDAFKFLMQ
MT*QFENFSVLLVFKMSCFKYKTNGKGDYLLFRW
LNIIFALDSFVIIFLQTVQHF*KMT*IFIKNPF
PMFIVYKNYAIKFLYKN
Frame 2
RSRGGRERGSDTGGGCLAFP*ARGGPSRAPSRSASPSASASAWPRLWEVRSPLLPLFFASRFVGED
ITVMSAFNLLHLVTKSQPVALRACGLPSGSCRD
KKNCKVVFSQQELRKRLTPLQYHVTQEKGTESAF
EGEYTHHKDPGIYKCVVCGTPLFKSETKFDSGS
GWPSFHDVINSEAITFTDDFSYGMHRVETSCSQ
CGAHLGHIFDDGPRPTGKRYCINSAALSFTPADS
SGTAEGGSGVASPAQADKAEL*SNGE*WKQSVL
NAQLIKKIKIVILIDIFFQKL*GQFCAIDIFSS
FA*TEALAIHVFCN*LDQELFIALANGDSFVKLL
HKPLIPFGILFFEHMASFAVFPPLIQKQRVHGL
QT*K*RSPLQCRDQSWAVQGMETCKTHGLEAFA
QTHLR*GWSDVLMRLFSFVESLS*GHFFFSH*KG
VKV*SLSLC*IVARSKGAY*VLWQRSKHYHTVR
PLFYFIFHRKHWRPSAMAHACDPSTLGGQGGWI
TRSGDGDHPG*HGETPSLLKIRKISQAWWWAPVV
PATQEAEAGEWREPGRRSLQLAEIMPLHSSLHD
NVTLHLKK****QYKN*LGMVAHACSPSYS*GS
VRRIA*TWEAEVAVS*AHTTALQPEQSEILSKKK
RKRKQHSNVRQL*NIEPHLV*NSKRRTPVHRLF
ISPSLHT*KQLQLEGHKFHCVK*NIRGNTLHQG
SRNEREQRRSRMKSWVLKNSVLGRKTDRAVQVTN
RIQVGWPLCTELQVTSGETWAFTWKAKTEAQVS
YST*SSV*QRTSHVASLCLFLHA*TGTHNICKM
Y*YSQGKFTILLYS*DQCCKIKLIWF*LPQRPKP
RHLKWKEAERRLT*LIGMETVGPRARISLCSNT
ASFTLRSSAQLLYNIKSGGMDVTVHNKVFTSIN
NGDVSQHCCSQV*RLYGV*RMYIMFVGLDANLEL
*FKKYVFL*LFESL*RLTAFLVSTKRKKRKRDI
*QF*RNNKKLE*KSVTNSVSVAMSTFLHVSLYK
HLSKKPDIPVDSL*SCTLDSR*ALVLSGCSDKRF
LSEHPDVPSGSKTFHPRPWVHSGIRRLHVSLEM
TCKI*DQTSAISVQTILEVFPNLTEKALVFPSD
PRIPRWGGQRNRSLRALPQPPAHPSEAHTAPLGC
SSSVG*PPLGSVGRGPQC*GRQGGSVRGLFPLH
RDWIVIVPSFIS*GLTFHSKKSPSFSNP*SLFQ
GKPVFLLPHFPGSTLRKMQHLLKKSGVYACGLIP
DCFWV*VVSNFSIFLSYVKEIYY*NVSEQ*CQL
SSTRFIFAGYGVQ*TESIWQGVCDLWELCHLT*
EVHGTFPLCTPALTVPLEDAEFCWCAKSIALHSS
RMDLKKAAISVTREHSHVFKLFTS*MHLNS*CK
*RSNLKTSPYYLCLKCLALNTKQMVKGIIFCLD
G*ILFLP*IAL**FFSRQFNTFEK*HEFSLKTLF
LCLLYTRIMQ*NFFIKI
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