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Sequences

Sequence NM_053028

GGCGCTGAGCGAGCTCGGAGCCCGCGCTGTGCGCCTGCGGCCGGGGCGCCCCGCCGAGCGCCGGTGCCCCGGCTCCCGGGCCGCCTTCGCCGCGCGGGAA GGATTCTTCAAAATTAACAGAAACCAATTCGGGCCAGCTGAAGAGAAAAAATAAAGGTGGCTCCCGGCTGCCTCTGCTGCAGTTCAGAGCAACTTCAGGA GCTTCCCAGCCGAGAGCTTCAGGACGCCTTTCCTGTCCCACTGGCCCAGTTGCCACAACAAACAACAGAGAAGACGGTGACCATGGGGGATGTGAAGCTG GTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTGGATCCCTCAAGAGTTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCTC GGAACCTCTGCATCAAAGAAGGAGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTACCCAGAGCCCCAGGTGACATGGCACAGAAACGGGCAACCCAT CACCAGCGGGGGCCGCTTCCTGCTGGATTGCGGCATCCGGGGGACTTTCAGCCTTGTGATTCATGCTGTCCATGAGGAGGACAGGGGAAAGTATACCTGT GAAGCCACCAATGGCAGTGGTGCTCGCCAGGTGACAGTGGAGTTGACAGTAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACCT TAGGGGATAGATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGCCCACCAAAGTTTGCTACCAAGCTGGGCCGAGTTGTGGTCAA AGAAGGACAGATGGGACGATTCTCCTGCAAGATCACTGGCCGGCCCCAACCGCAGGTCACCTGGCTCAAGGGAAATGTTCCACTGCAGCCGAGTGCCCGT GTGTCTGTGTCTGAGAAGAACGGCATGCAGGTTCTGGAAATCCATGGAGTCAACCAAGATGACGTGGGAGTGTACACGTGCCTGGTGGTGAACGGGTCGG GGAAGGCCTCGATGTCAGCTGAACTTTCCATCCAAGGTTTGGACAGTGCCAATAGGTCATTTGTGAGAGAAACAAAAGCCACCAATTCAGATGTCAGGAA AGAGGTGACCAATGTAATCTCAAAGGAGTCGAAGCTGGACAGTCTGGAGGCTGCAGCCAAAAGCAAGAACTGCTCCAGCCCCCAGAGAGGTGGCTCCCCA CCCTGGGCTGCAAACAGCCAGCCTCAGCCCCCAAGGGAGTCCAAGCTGGAGTCATGCAAGGACTCGCCCAGAACGGCCCCGCAGACCCCGGTCCTTCAGA AGACTTCCAGCTCCATCACCCTGCAGGCCGCAAGAGTTCAGCCGGAACCAAGAGCACCAGGCCTGGGGGTCCTATCACCTTCTGGAGAAGAGAGGAAGAG GCCAGCTCCTCCCCGTCCAGCCACCTTCCCCACCAGGCAGCCTGGCCTGGGGAGCCAAGATGTTGTGAGCAAGGCTGCTAACAGGAGAATCCCCATGGAG GGCCAGAGGGATTCAGCATTCCCCAAATTTGAGAGCAAGCCCCAAAGCCAGGAGGTCAAGGAAAATCAAACTGTCAAGTTCAGATGTGAAGGGCTTGCCG TGATGGAGGTGGCCCCCTCCTTCTCCAGTGTCCTGAAGGACTGCGCTGTTATTGAGGGCCAGGATTTTGTGCTGCAGTGCTCCGTACGGGGGACCCCAGT GCCCCGGATCACTTGGCTGCTGAATGGGCAGCCCATCCAGTACGCTCGCTCCACCTGCGAGGCCGGCGTGGCTGAGCTCCACATCCAGGATGCCCTGCCG GAGGACCATGGCACCTACACCTGCCTAGCTGAGAATGCCTTGGGGCAGGTGTCCTGCAGCGCCTGGGTCACCGTCCATGAAAAGAAGAGTAGCAGGAAGA GTGAGTACCTTCTGCCTGTGGCTCCCAGCAAGCCCACTGCACCCATCTTCCTGCAGGGCCTCTCTGATCTCAAAGTCATGGATGGAAGCCAGGTCACTAT GACTGTCCAAGTGTCAGGGAATCCACCCCCTGAAGTCATCTGGCTGCACAATGGGAATGAGATCCAAGAGTCAGAGGACTTCCACTTTGAACAGAGAGGA ACTCAGCACAGCCTTTGTATCCAGGAAGTGTTCCCGGAGGACACGGGCACGTACACCTGCGAGGCCTGGAACAGCGCTGGAGAGGTCCGCACCCAGGCCG TGCTCACGGTACAAGAGCCTCACGATGGCACCCAGCCCTGGTTCATCAGTAAGCCTCGCTCAGTGACAGCCTCCCTGGGCCAGAGTGTCCTCATCTCCTG CGCCATAGCTGGTGACCCCTTTCCTACCGTGCACTGGCTCAGAGATGGCAAAGCCCTCTGCAAAGACACTGGCCACTTCGAGGTGCTTCAGAATGAGGAC GTGTTCACCCTGGTTCTAAAGAAGGTGCAGCCCTGGCATGCCGGCCAGTATGAGATCCTGCTCAAGAACCGGGTTGGCGAATGCAGTTGCCAGGTGTCAC TGATGCTACAGAACAGCTCTGCCAGAGCCCTTCCACGGGGGAGGGAGCCTGCCAGCTGCGAGGACCTCTGTGGTGGAGGAGTTGGTGCTGATGGTGGTGG TAGTGACCGCTATGGGTCCCTGAGGCCTGGCTGGCCAGCAAGAGGGCAGGGTTGGCTAGAGGAGGAAGACGGCGAGGACGTGCGAGGGGTGCTGAAGAGG CGCGTGGAGACGAGGCAGCACACTGAGGAGGCGATCCGCCAGCAGGAGGTGGAGCAGCTGGACTTCCGAGACCTCCTGGGGAAGAAGGTGAGTACAAAGA CCCTATCGGAAGACGACCTGAAGGAGATCCCAGCCGAGCAGATGGATTTCCGTGCCAACCTGCAGCGGCAAGTGAAGCCAAAGACTGTGTCTGAGGAAGA GAGGAAGGTGCACAGCCCCCAGCAGGTCGATTTTCGCTCTGTCCTGGCCAAGAAGGGGACTTCCAAGACCCCCGTGCCTGAGAAGGTGCCACCGCCAAAA CCTGCCACCCCGGATTTTCGCTCAGTGCTGGGTGGCAAGAAGAAATTACCAGCAGAGAATGGCAGCAGCAGTGCCGAGACCCTGAATGCCAAGGCAGTGG AGAGTTCCAAGCCCCTGAGCAATGCACAGCCTTCAGGGCCCTTGAAACCCGTGGGCAACGCCAAGCCTGCTGAGACCCTGAAGCCAATGGGCAACGCCAA GCCTGCCGAGACCCTGAAGCCCATGGGCAATGCCAAGCCTGATGAGAACCTGAAATCCGCTAGCAAAGAAGAACTCAAGAAAGACGTTAAGAATGATGTG AACTGCAAGAGAGGCCATGCAGGGACCACAGATAATGAAAAGAGATCAGAGAGCCAGGGGACAGCCCCAGCCTTCAAGCAGAAGCTGCAAGATGTTCATG TGGCAGAGGGCAAGAAGCTGCTGCTCCAGTGCCAGGTGTCTTCTGACCCCCCAGCCACCATCATCTGGACGCTGAACGGAAAGACCCTCAAGACCACCAA GTTCATCATCCTCTCCCAGGAAGGCTCACTCTGCTCCGTCTCCATCGAGAAGGCACTGCCTGAGGACAGAGGCTTATACAAGTGTGTAGCCAAGAATGAC GCTGGCCAGGCGGAGTGCTCCTGCCAAGTCACCGTGGATGATGCTCCAGCCAGTGAGAACACCAAGGCCCCAGAGATGAAATCCCGGAGGCCCAAGAGCT CTCTTCCTCCCGTGCTAGGAACTGAGAGTGATGCGACTGTGAAAAAGAAACCTGCCCCCAAGACACCTCCGAAGGCAGCAATGCCCCCTCAGATCATCCA GTTCCCTGAGGACCAGAAGGTACGCGCAGGAGAGTCAGTGGAGCTGTTTGGCAAAGTGACAGGCACTCAGCCCATCACCTGTACCTGGATGAAGTTCCGA AAGCAGATCCAGGAAAGCGAGCACATGAAGGTGGAGAACAGCGAGAATGGCAGCAAGCTCACCATCCTGGCCGCGCGCCAGGAGCACTGCGGCTGCTACA CACTGCTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAGGTCAACCTCACTGTCGTGGATAAGCCAGACCCCCCAGCTGGCACACCTTGTGCCTCTGA CATTCGGAGCTCCTCACTGACCCTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAGCATCGAGATCTGGGACTCAGCCAAC AAGACGTGGAAGGAACTAGCCACATGCCGCAGCACCTCTTTCAACGTCCAGGACCTGCTGCCTGACCACGAATATAAGTTCCGTGTACGTGCAATCAACG TGTATGGAACCAGTGAGCCAAGCCAGGAGTCTGAACTCACAACGGTAGGAGAGAAACCTGAAGAGCCGAAGGATGAAGTGGAGGTGTCAGATGATGATGA GAAGGAGCCCGAGGTTGATTACCGGACAGTGACAATCAATACTGAACAAAAAGTATCTGACTTCTACGACATTGAGGAGAGATTAGGATCTGGGAAATTT GGACAGGTCTTTCGACTTGTAGAAAAGAAAACTCGAAAAGTCTGGGCAGGGAAGTTCTTCAAGGCATATTCAGCAAAAGAGAAAGAGAATATCCGGCAGG AGATTAGCATCATGAACTGCCTCCACCACCCTAAGCTGGTCCAGTGTGTGGATGCCTTTGAAGAAAAGGCCAACATCGTCATGGTCCTGGAGATCGTGTC AGGAGGGGAGCTGTTTGAGCGCATCATTGACGAGGACTTTGAGCTGACGGAGCGTGAGTGCATCAAGTACATGCGGCAGATCTCGGAGGGAGTGGAGTAC ATCCACAAGCAGGGCATCGTGCACCTGGACCTCAAGCCGGAGAACATCATGTGTGTCAACAAGACGGGCACCAGGATCAAGCTCATCGACTTTGGTCTGG CCAGGAGGCTGGAGAATGCGGGGTCTCTGAAGGTCCTCTTTGGCACCCCAGAATTTGTGGCTCCTGAAGTGATCAACTATGAGCCCATCGGCTACGCCAC AGACATGTGGAGCATCGGGGTCATCTGCTACATCCTAAACCGCCTGGACTGCACGCAGTGCCTTCAGCATCCATGGCTAATGAAAGATACCAAGAACATG GAGGCCAAGAAACTCTCCAAGGACCGGATGAAGAAGTACATGGCAAGAAGGAAATGGCAGAAAACGGGCAATGCTGTGAGAGCCATTGGAAGACTGTCCT CTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCTCAACAGGGTCACCAACCAGCCCGCTCAATGCAGAAAAACTAGAATCTGAAGAAGATGTGTC CCAAGCTTTCCTTGAGGCTGTTGCTGAGGAAAAGCCTCATGTAAAACCCTATTTCTCTAAGACCATTCGCGATTTAGAAGTTGTGGAGGGAAGTGCTGCT AGATTTGACTGCAAGATTGAAGGATACCCAGACCCCGAGGTTGTCTGGTTCAAAGATGACCAGTCAATCAGGGAGTCCCGCCACTTCCAGATAGACTACG ATGAGGACGGGAACTGCTCTTTAATTATTAGTGATGTTTGCGGGGATGACGATGCCAAGTACACCTGCAAGGCTGTCAACAGTCTTGGAGAAGCCACCTG CACAGCAGAGCTCATTGTGGAAACGATGGAGGAAGGTGAAGGGGAAGGGGAAGAGGAAGAAGAGTGAAACAAAGCCAGAGAAAAGCAGTTTCTAAGTCAT ATTAAAAGGACTATTTCTCTAAAACTCAAAAAAAAAAAAAAAACTCAAGATAGTAAAAGCACCTAGTGTGATAGATTATCGGTTAGGTCATTTGTGGGTT GATTCTTCAGAAACAGCAGTTGATACCTAGCAGCGTTATTGATGGGCATTAATCTATGTTAGTTGGCACCTTAAGATACTAGTGCAGCTAGATTTCATTT AGGGAAATCACCAGTAACTTGACTGACCAATTGATTTTAGAGAGAAAGTAACCAAACCAAATATTTATCTGGGCAAAGTCATAAATTCTCCACTTGAATG CGCTCATGAAAAATAAGGCCAAAACAAGAGTTCTGGGCCACAGCTCAGCCCAGAGGGTTCCTGGGGATGGGAGGCCTCTCTCTCCCCACCCCCTGACTCT AGAGAACTGGGTTTTCTCCCAGTACTCCAGCAATTCATTTCTGAAAGCAGTTGAGCCACTTTATTCCAAAGTACACTGCAGATGTTCAAACTCTCCATTT CTCTTTCCCCTTCCACCTGCCAGTTTTGCTGACTCTCAACTTGTCATGAGTGTAAGCATTAAGGACATTATGCTTCTTCGATTCTGAAGACAGGTCCCTG CTCATGGATGACTCTGGCTTCCTTAGGAAAATATTTTTCTTCCAAAATCAGTAGGAAATCTAAACTTATCCCCTCTTTGCAGATGTCTAGCAGCTTCAGA CATTTGGTTAAGAACCCATGGGAAAAAAAAAATCCTTGCTAATGTGGTTTCCTTTGTAAACCAGGATTCTTATTTGTGCTGTTATAGAATATCAGCTCTG AACGTGTGGTAAAGATTTTTGTGTTTGAATATAGGAGAAATCAGTTTGCTGAAAAGTTAGTCTTAATTATCTATTGGCCACGATGAAACAGATTTCAACT GATAAAGAGCTGGAGAACTCCATGTACTTTGGAATCTCCTCCAAGATAGCCAGAGTTTAATACATCTTCATTCTCAACACTCTCCAAAGAACTTGACCTA CCTTATGGGTTCCATATTTTTCTTCTTAAATGTGCATCAATCATGCCTTGCCCCCAACCTTTAAATATATTCTTAGACCTGGTAAATGCACTCAGACTTG CGTCTTTAGGAATTTTTAACTTTCTTTCACTACATTGGCACTTAAATTTTTTCTTTATAAAGCTTTTTGAAGGTCATAAACAAAGACCATAATTGATGAT AGACCTAATACATTTCCTCTGTGTGTGTGTGTAACATTCCAAATACTTTTTTTTTCTTTTCCACTGTTTGTAAGGTGCAACAATTTAATATTTTTAAGGG ACTTTTTAAGAGTTCCTTAAGAACCAATTTAAAATTACTTCAGTGCAATCCTACACAGTATCAACATTAGAATTTTGATATTAGTCTTATGTTATCTTCC ATTCTATTTTTATCTGCTTTTTGCTGCTAGTTTCAAACTGCCAGTATTTTTCCTTTTGCTTTTAAAATAGTTACAATATTTTTCATGATAGCCACAGTAT TGCCACAGTTTATTATAATAAAGGGTTTTTATTTGATTTAGCGCATTCAAAGCTTTTTTCTATCACTTTTGTGTTCAGAATATAACCTTTGTGTGCGTGT ATGTTGTGTGTGTGCATGTGTGGCGTATATGTGTGTTACAGGTTAATGCCTTCTTGGAATTGTGTTAATGTTCTCTTGGTTTATTATGCCATCAGAATGG TAAATGAGAACACTACAACTGTAGTCAGCTCACAATTTTTAAATAAAGGATACCACAGTGCATGCTGTTTGTTCAA

refseq_MYLK.F4 (forward primer)
refseq_MYLK.R4 (reverse primer)
Single exon skipping

Frame 0

GAERARSPRCAPAAGAPRRAPVPRLPGRLRRAG RILQN*QKPIRAS*REKIKVAPGCLCCSSEQLQE LPSRELQDAFPVPLAQLPQQTTEKTVTMGDVKL VASSHISKTSLSVDPSRVDSMPLTEAPAFILPP RNLCIKEGATAKFEGRVRGYPEPQVTWHRNGQPI TSGGRFLLDCGIRGTFSLVIHAVHEEDRGKYTC EATNGSGARQVTVELTVEGSFAKQLGQPVVSKT LGDRFSAPAVETRPSIWGECPPKFATKLGRVVVK EGQMGRFSCKITGRPQPQVTWLKGNVPLQPSAR VSVSEKNGMQVLEIHGVNQDDVGVYTCLVVNGS GKASMSAELSIQGLDSANRSFVRETKATNSDVRK EVTNVISKESKLDSLEAAAKSKNCSSPQRGGSP PWAANSQPQPPRESKLESCKDSPRTAPQTPVLQ KTSSSITLQAARVQPEPRAPGLGVLSPSGEERKR PAPPRPATFPTRQPGLGSQDVVSKAANRRIPME GQRDSAFPKFESKPQSQEVKENQTVKFRCEGLA VMEVAPSFSSVLKDCAVIEGQDFVLQCSVRGTPV PRITWLLNGQPIQYARSTCEAGVAELHIQDALP EDHGTYTCLAENALGQVSCSAWVTVHEKKSSRK SEYLLPVAPSKPTAPIFLQGLSDLKVMDGSQVTM TVQVSGNPPPEVIWLHNGNEIQESEDFHFEQRG TQHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQA VLTVQEPHDGTQPWFISKPRSVTASLGQSVLISC AIAGDPFPTVHWLRDGKALCKDTGHFEVLQNED VFTLVLKKVQPWHAGQYEILLKNRVGECSCQVS LMLQNSSARALPRGREPASCEDLCGGGVGADGGG SDRYGSLRPGWPARGQGWLEEEDGEDVRGVLKR RVETRQHTEEAIRQQEVEQLDFRDLLGKKVSTK TLSEDDLKEIPAEQMDFRANLQRQVKPKTVSEEE RKVHSPQQVDFRSVLAKKGTSKTPVPEKVPPPK PATPDFRSVLGGKKKLPAENGSSSAETLNAKAV ESSKPLSNAQPSGPLKPVGNAKPAETLKPMGNAK PAETLKPMGNAKPDENLKSASKEELKKDVKNDV NCKRGHAGTTDNEKRSESQGTAPAFKQKLQDVH VAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTK FIILSQEGSLCSVSIEKALPEDRGLYKCVAKND AGQAECSCQVTVDDAPASENTKAPEMKSRRPKS SLPPVLGTESDATVKKKPAPKTPPKAAMPPQIIQ FPEDQKVRAGESVELFGKVTGTQPITCTWMKFR KQIQESEHMKVENSENGSKLTILAARQEHCGCY TLLVENKLGSRQAQVNLTVVDKPDPPAGTPCASD IRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSAN KTWKELATCRSTSFNVQDLLPDHEYKFRVRAIN VYGTSEPSQESELTTVGEKPEEPKDEVEVSDDDE KEPEVDYRTVTINTEQKVSDFYDIEERLGSGKF GQVFRLVEKKTRKVWAGKFFKAYSAKEKENIRQ EISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVS GGELFERIIDEDFELTERECIKYMRQISEGVEY IHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGL ARRLENAGSLKVLFGTPEFVAPEVINYEPIGYAT DMWSIGVICYILNRLDCTQCLQHPWLMKDTKNM EAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLS SMAMISGLSGRKSSTGSPTSPLNAEKLESEEDVS QAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAA RFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDY DEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATC TAELIVETMEEGEGEGEEEEE*NKAREKQFLSH IKRTISLKLKKKKKTQDSKST*CDRLSVRSFVG *FFRNSS*YLAALLMGINLC*LAP*DTSAARFHL GKSPVT*LTN*F*RESNQTKYLSGQSHKFST*M RS*KIRPKQEFWATAQPRGFLGMGGLSLPTP*L *RTGFSPSTPAIHF*KQLSHFIPKYTADVQTLHF SFPFHLPVLLTLNLS*V*ALRTLCFFDSEDRSL LMDDSGFLRKIFFFQNQ*EI*TYPLFADV*QLQ TFG*EPMGKKKSLLMWFPL*TRILICAVIEYQL* TCGKDFCV*I*EKSVC*KVSLNYLLATMKQIST DKELENSMYFGISSKIARV*YIFILNTLQRT*P TLWVPYFSS*MCINHALPPTFKYILRPGKCTQTC VFRNF*LSFTTLALKFFLYKAF*RS*TKTIIDD RPNTFPLCVCVTFQILFFSFPLFVRCNNLIFLR DFLRVP*EPI*NYFSAILHSINIRILILVLCYLP FYFYLLFAASFKLPVFFLLLLK*LQYFS**PQY CHSLL**RVFI*FSAFKAFFYHFCVQNITFVCV YVVCVHVWRICVLQVNAFLELC*CSLGLLCHQNG K*EHYNCSQLTIFK*RIPQCMLFVQ

Frame 1

ALSELGARAVRLRPGRPAERRCPGSRAAFAARE GFFKINRNQFGPAEEKK*RWLPAASAAVQSNFR SFPAESFRTPFLSHWPSCHNKQQRRR*PWGM*SW LPRHTFPKPPSVWIPQELTPCP*QRPLLSFCPL GTSASKKEPPPSSKGGSGVTQSPR*HGTETGNP SPAGAASCWIAASGGLSAL*FMLSMRRTGESIPV KPPMAVVLAR*QWS*Q*KEVLRSSLVSLLFPKP *GIDFQLQQWRPVLASGGSAHQSLLPSWAELWS KKDRWDDSPARSLAGPNRRSPGSREMFHCSRVPV CLCLRRTACRFWKSMESTKMTWECTRAWW*TGR GRPRCQLNFPSKVWTVPIGHL*EKQKPPIQMSG KR*PM*SQRSRSWTVWRLQPKARTAPAPREVAPH PGLQTASLSPQGSPSWSHARTRPERPRRPRSFR RLPAPSPCRPQEFSRNQEHQAWGSYHLLEKRGR GQLLPVQPPSPPGSLAWGAKML*ARLLTGESPWR ARGIQHSPNLRASPKARRSRKIKLSSSDVKGLP *WRWPPPSPVS*RTALLLRARILCCSAPYGGPQ CPGSLGC*MGSPSSTLAPPARPAWLSSTSRMPCR RTMAPTPA*LRMPWGRCPAAPGSPSMKRRVAGR VSTFCLWLPASPLHPSSCRASLISKSWMEARSL *LSKCQGIHPLKSSGCTMGMRSKSQRTSTLNREE LSTAFVSRKCSRRTRARTPARPGTALERSAPRP CSRYKSLTMAPSPGSSVSLAQ*QPPWARVSSSP AP*LVTPFLPCTGSEMAKPSAKTLATSRCFRMRT CSPWF*RRCSPGMPASMRSCSRTGLANAVARCH *CYRTALPEPFHGGGSLPAARTSVVEELVLMVV VVTAMGP*GLAGQQEGRVG*RRKTARTCEGC*RG AWRRGSTLRRRSASRRWSSWTSETSWGRR*VQR PYRKTT*RRSQPSRWISVPTCSGK*SQRLCLRK RGRCTAPSRSIFALSWPRRGLPRPPCLRRCHRQN LPPRIFAQCWVARRNYQQRMAAAVPRP*MPRQW RVPSP*AMHSLQGP*NPWATPSLLRP*SQWATP SLPRP*SPWAMPSLMRT*NPLAKKNSRKTLRMM* TAREAMQGPQIMKRDQRARGQPQPSSRSCKMFM WQRARSCCSSARCLLTPQPPSSGR*TERPSRPP SSSSSPRKAHSAPSPSRRHCLRTEAYTSV*PRMT LARRSAPAKSPWMMLQPVRTPRPQR*NPGGPRA LFLPC*ELRVMRL*KRNLPPRHLRRQQCPLRSS SSLRTRRYAQESQWSCLAK*QALSPSPVPG*SSE SRSRKAST*RWRTARMAASSPSWPRARSTAAAT HCWWRTSWAAGRPRSTSLSWISQTPQLAHLVPL TFGAPH*PCPGMAPHMMGAVLYSPTASRSGTQPT RRGRN*PHAAAPLSTSRTCCLTTNISSVYVQST CMEPVSQARSLNSQR*ERNLKSRRMKWRCQMMM RRSPRLITGQ*QSILNKKYLTSTTLRRD*DLGNL DRSFDL*KRKLEKSGQGSSSRHIQQKRKRISGR RLAS*TASTTLSWSSVWMPLKKRPTSSWSWRSC QEGSCLSASLTRTLS*RSVSASSTCGRSRREWST STSRASCTWTSSRRTSCVSTRRAPGSSSSTLVW PGGWRMRGL*RSSLAPQNLWLLK*STMSPSATP QTCGASGSSATS*TAWTARSAFSIHG**KIPRTW RPRNSPRTG*RSTWQEGNGRKRAML*EPLEDCP LWQ*SQGSVAGNPQQGHQPARSMQKN*NLKKMC PKLSLRLLLRKSLM*NPISLRPFAI*KLWREVLL DLTARLKDTQTPRLSGSKMTSQSGSPATSR*TT MRTGTAL*LLVMFAGMTMPSTPARLSTVLEKPP AQQSSLWKRWRKVKGKGKRKKSETKPEKSSF*VI LKGLFL*NSKKKKKLKIVKAPSVIDYRLGHLWV DSSETAVDT*QRY*WALIYVSWHLKILVQLDFI *GNHQ*LD*PIDFREKVTKPNIYLGKVINSPLEC AHEK*GQNKSSGPQLSPEGSWGWEASLSPPPDS RELGFLPVLQQFISESS*ATLFQSTLQMFKLSI SLSPSTCQFC*LSTCHECKH*GHYASSILKTGPC SWMTLASLGKYFSSKISRKSKLIPSLQMSSSFR HLVKNPWEKKNPC*CGFLCKPGFLFVLL*NISS ERVVKIFVFEYRRNQFAEKLVLIIYWPR*NRFQL IKSWRTPCTLESPPR*PEFNTSSFSTLSKELDL PYGFHIFLLKCASIMPCPQPLNIFLDLVNALRL ASLGIFNFLSLHWHLNFFFIKLFEGHKQRP*LMI DLIHFLCVCV*HSKYFFFLFHCL*GATI*YF*G TF*EFLKNQFKITSVQSYTVSTLEF*Y*SYVIF HSIFICFLLLVSNCQYFSFCF*NSYNIFHDSHSI ATVYYNKGFLFDLAHSKLFSITFVFRI*PLCAC MLCVCMCGVYVCYRLMPSWNCVNVLLVYYAIRM VNENTTTVVSSQFLNKGYHSACCLF

Frame 2

R*ASSEPALCACGRGAPPSAGAPAPGPPSPRGK DSSKLTETNSGQLKRKNKGGSRLPLLQFRATSG ASQPRASGRLSCPTGPVATTNNREDGDHGGCEA GCLVTHFQNLPQCGSLKS*LHAPDRGPCFHFAPS EPLHQRRSHRQVRRAGPGLPRAPGDMAQKRATH HQRGPLPAGLRHPGDFQPCDSCCP*GGQGKVYL *SHQWQWCSPGDSGVDSRRKFCEAAWSACCFQNL RG*IFSSSSGDPS*HLGGVPTKVCYQAGPSCGQ RRTDGTILLQDHWPAPTAGHLAQGKCSTAAECP CVCV*EERHAGSGNPWSQPR*RGSVHVPGGERVG EGLDVS*TFHPRFGQCQ*VICERNKSHQFRCQE RGDQCNLKGVEAGQSGGCSQKQELLQPPERWLP TLGCKQPASAPKGVQAGVMQGLAQNGPADPGPSE DFQLHHPAGRKSSAGTKSTRPGGPITFWRREEE ASSSPSSHLPHQAAWPGEPRCCEQGC*QENPHG GPEGFSIPQI*EQAPKPGGQGKSNCQVQM*RACR DGGGPLLLQCPEGLRCY*GPGFCAAVLRTGDPS APDHLAAEWAAHPVRSLHLRGRRG*APHPGCPA GGPWHLHLPS*ECLGAGVLQRLGHRP*KEE*QEE *VPSACGSQQAHCTHLPAGPL*SQSHGWKPGHY DCPSVRESTP*SHLAAQWE*DPRVRGLPL*TER NSAQPLYPGSVPGGHGHVHLRGLEQRWRGPHPGR AHGTRASRWHPALVHQ*ASLSDSLPGPECPHLL RHSW*PLSYRALAQRWQSPLQRHWPLRGASE*G RVHPGSKEGAALACRPV*DPAQEPGWRMQLPGVT DATEQLCQSPSTGEGACQLRGPLWWRSWC*WWW **PLWVPEAWLASKRAGLARGGRRRGRARGAEE ARGDEAAH*GGDPPAGGGAAGLPRPPGEEGEYKD PIGRRPEGDPSRADGFPCQPAAASEAKDCV*GR EEGAQPPAGRFSLCPGQEGDFQDPRA*EGATAK TCHPGFSLSAGWQEEITSREWQQQCRDPECQGSG EFQAPEQCTAFRALETRGQRQAC*DPEANGQRQ ACRDPEAHGQCQA**EPEIR*QRRTQERR*E*C ELQERPCRDHR**KEIREPGDSPSLQAEAARCSC GRGQEAAAPVPGVF*PPSHHHLDAERKDPQDHQ VHHPLPGRLTLLRLHREGTA*GQRLIQVCSQE* RWPGGVLLPSHRG*CSSQ*EHQGPRDEIPEAQEL SSSRARN*E*CDCEKETCPQDTSEGSNAPSDHP VP*GPEGTRRRVSGAVWQSDRHSAHHLYLDEVP KADPGKRAHEGGEQREWQQAHHPGRAPGALRLLH TAGGEQAGQQAGPGQPHCRG*ARPPSWHTLCL* HSELLTDPVLVWLLI*WGQCCTVLQHRDLGLSQ QDVEGTSHMPQHLFQRPGPAA*PRI*VPCTCNQR VWNQ*AKPGV*THNGRRET*RAEG*SGGVR*** EGARG*LPDSDNQY*TKSI*LLRH*GEIRIWEI WTGLSTCRKENSKSLGREVLQGIFSKREREYPAG D*HHELPPPP*AGPVCGCL*RKGQHRHGPGDRV RRGAV*AHH*RGL*ADGA*VHQVHAADLGGSGV HPQAGHRAPGPQAGEHHVCQQDGHQDQAHRLWSG QEAGECGVSEGPLWHPRICGS*SDQL*AHRLRH RHVEHRGHLLHPKPPGLHAVPSASMANERYQEH GGQETLQGPDEEVHGKKEMAENGQCCESHWKTVL YGNDLRAQWQEILNRVTNQPAQCRKTRI*RRCV PSFP*GCC*GKASCKTLFL*DHSRFRSCGGKCC *I*LQD*RIPRPRGCLVQR*PVNQGVPPLPDRLR *GRELLFNY**CLRG*RCQVHLQGCQQSWRSHL HSRAHCGNDGGR*RGRGRGRRVKQSQRKAVSKS Y*KDYFSKTQKKKKNSR**KHLV**IIG*VICGL ILQKQQLIPSSVIDGH*SMLVGTLRY*CS*ISF REITSNLTDQLILERK*PNQIFIWAKS*ILHLN ALMKNKAKTRVLGHSSAQRVPGDGRPLSPHPLTL ENWVFSQYSSNSFLKAVEPLYSKVHCRCSNSPF LFPLPPASFADSQLVMSVSIKDIMLLRF*RQVP AHG*LWLP*ENIFLPKSVGNLNLSPLCRCLAASD IWLRTHGKKKILANVVSFVNQDSYLCCYRISAL NVW*RFLCLNIGEISLLKS*S*LSIGHDETDFN **RAGELHVLWNLLQDSQSLIHLHSQHSPKNLTY LMGSIFFFLNVHQSCLAPNL*IYS*TW*MHSDL RL*EFLTFFHYIGT*IFSL*SFLKVINKDHN** *T*YISSVCVCNIPNTFFFFSTVCKVQQFNIFKG LFKSSLRTNLKLLQCNPTQYQH*NFDISLMLSS ILFLSAFCC*FQTASIFPFAFKIVTIFFMIATV LPQFIIIKGFYLI*RIQSFFLSLLCSEYNLCVRV CCVCACVAYMCVTG*CLLGIVLMFSWFIMPSEW *MRTLQL*SAHNF*IKDTTVHAVCS

Sequence NM_053026

GGCGCTGAGCGAGCTCGGAGCCCGCGCTGTGCGCCTGCGGCCGGGGCGCCCCGCCGAGCGCCGGTGCCCCGGCTCCCGGGCCGCCTTCGCCGCGCGGGAA GGATTCTTCAAAATTAACAGAAACCAATTCGGGCCAGCTGAAGAGAAAAAATAAAGGTGGCTCCCGGCTGCCTCTGCTGCAGTTCAGAGCAACTTCAGGA GCTTCCCAGCCGAGAGCTTCAGGACGCCTTTCCTGTCCCACTGGCCCAGTTGCCACAACAAACAACAGAGAAGACGGTGACCATGGGGGATGTGAAGCTG GTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTGGATCCCTCAAGAGTTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCTC GGAACCTCTGCATCAAAGAAGGAGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTACCCAGAGCCCCAGGTGACATGGCACAGAAACGGGCAACCCAT CACCAGCGGGGGCCGCTTCCTGCTGGATTGCGGCATCCGGGGGACTTTCAGCCTTGTGATTCATGCTGTCCATGAGGAGGACAGGGGAAAGTATACCTGT GAAGCCACCAATGGCAGTGGTGCTCGCCAGGTGACAGTGGAGTTGACAGTAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACCT TAGGGGATAGATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGCCCACCAAAGTTTGCTACCAAGCTGGGCCGAGTTGTGGTCAA AGAAGGACAGATGGGACGATTCTCCTGCAAGATCACTGGCCGGCCCCAACCGCAGGTCACCTGGCTCAAGGGAAATGTTCCACTGCAGCCGAGTGCCCGT GTGTCTGTGTCTGAGAAGAACGGCATGCAGGTTCTGGAAATCCATGGAGTCAACCAAGATGACGTGGGAGTGTACACGTGCCTGGTGGTGAACGGGTCGG GGAAGGCCTCGATGTCAGCTGAACTTTCCATCCAAGGTTTGGACAGTGCCAATAGGTCATTTGTGAGAGAAACAAAAGCCACCAATTCAGATGTCAGGAA AGAGGTGACCAATGTAATCTCAAAGGAGTCGAAGCTGGACAGTCTGGAGGCTGCAGCCAAAAGCAAGAACTGCTCCAGCCCCCAGAGAGGTGGCTCCCCA CCCTGGGCTGCAAACAGCCAGCCTCAGCCCCCAAGGGAGTCCAAGCTGGAGTCATGCAAGGACTCGCCCAGAACGGCCCCGCAGACCCCGGTCCTTCAGA AGACTTCCAGCTCCATCACCCTGCAGGCCGCAAGAGTTCAGCCGGAACCAAGAGCACCAGGCCTGGGGGTCCTATCACCTTCTGGAGAAGAGAGGAAGAG GCCAGCTCCTCCCCGTCCAGCCACCTTCCCCACCAGGCAGCCTGGCCTGGGGAGCCAAGATGTTGTGAGCAAGGCTGCTAACAGGAGAATCCCCATGGAG GGCCAGAGGGATTCAGCATTCCCCAAATTTGAGAGCAAGCCCCAAAGCCAGGAGGTCAAGGAAAATCAAACTGTCAAGTTCAGATGTGAAGGGCTTGCCG TGATGGAGGTGGCCCCCTCCTTCTCCAGTGTCCTGAAGGACTGCGCTGTTATTGAGGGCCAGGATTTTGTGCTGCAGTGCTCCGTACGGGGGACCCCAGT GCCCCGGATCACTTGGCTGCTGAATGGGCAGCCCATCCAGTACGCTCGCTCCACCTGCGAGGCCGGCGTGGCTGAGCTCCACATCCAGGATGCCCTGCCG GAGGACCATGGCACCTACACCTGCCTAGCTGAGAATGCCTTGGGGCAGGTGTCCTGCAGCGCCTGGGTCACCGTCCATGAAAAGAAGAGTAGCAGGAAGA GTGAGTACCTTCTGCCTGTGGCTCCCAGCAAGCCCACTGCACCCATCTTCCTGCAGGGCCTCTCTGATCTCAAAGTCATGGATGGAAGCCAGGTCACTAT GACTGTCCAAGTGTCAGGGAATCCACCCCCTGAAGTCATCTGGCTGCACAATGGGAATGAGATCCAAGAGTCAGAGGACTTCCACTTTGAACAGAGAGGA ACTCAGCACAGCCTTTGTATCCAGGAAGTGTTCCCGGAGGACACGGGCACGTACACCTGCGAGGCCTGGAACAGCGCTGGAGAGGTCCGCACCCAGGCCG TGCTCACGGTACAAGAGCCTCACGATGGCACCCAGCCCTGGTTCATCAGTAAGCCTCGCTCAGTGACAGCCTCCCTGGGCCAGAGTGTCCTCATCTCCTG CGCCATAGCTGGTGACCCCTTTCCTACCGTGCACTGGCTCAGAGATGGCAAAGCCCTCTGCAAAGACACTGGCCACTTCGAGGTGCTTCAGAATGAGGAC GTGTTCACCCTGGTTCTAAAGAAGGTGCAGCCCTGGCATGCCGGCCAGTATGAGATCCTGCTCAAGAACCGGGTTGGCGAATGCAGTTGCCAGGTGTCAC TGATGCTACAGAACAGCTCTGCCAGAGCCCTTCCACGGGGGAGGGAGCCTGCCAGCTGCGAGGACCTCTGTGGTGGAGGAGTTGGTGCTGATGGTGGTGG TAGTGACCGCTATGGGTCCCTGAGGCCTGGCTGGCCAGCAAGAGGGCAGGGTTGGCTAGAGGAGGAAGACGGCGAGGACGTGCGAGGGGTGCTGAAGAGG CGCGTGGAGACGAGGCAGCACACTGAGGAGGCGATCCGCCAGCAGGAGGTGGAGCAGCTGGACTTCCGAGACCTCCTGGGGAAGAAGGTGAGTACAAAGA CCCTATCGGAAGACGACCTGAAGGAGATCCCAGCCGAGCAGATGGATTTCCGTGCCAACCTGCAGCGGCAAGTGAAGCCAAAGACTGTGTCTGAGGAAGA GAGGAAGGTGCACAGCCCCCAGCAGGTCGATTTTCGCTCTGTCCTGGCCAAGAAGGGGACTTCCAAGACCCCCGTGCCTGAGAAGGTGCCACCGCCAAAA CCTGCCACCCCGGATTTTCGCTCAGTGCTGGGTGGCAAGAAGAAATTACCAGCAGAGAATGGCAGCAGCAGTGCCGAGACCCTGAATGCCAAGGCAGTGG AGAGTTCCAAGCCCCTGAGCAATGCACAGCCTTCAGGGCCCTTGAAACCCGTGGGCAACGCCAAGCCTGCTGAGACCCTGAAGCCAATGGGCAACGCCAA GCCTGCCGAGACCCTGAAGCCCATGGGCAATGCCAAGCCTGATGAGAACCTGAAATCCGCTAGCAAAGAAGAACTCAAGAAAGACGTTAAGAATGATGTG AACTGCAAGAGAGGCCATGCAGGGACCACAGATAATGAAAAGAGATCAGAGAGCCAGGGGACAGCCCCAGCCTTCAAGCAGAAGCTGCAAGATGTTCATG TGGCAGAGGGCAAGAAGCTGCTGCTCCAGTGCCAGGTGTCTTCTGACCCCCCAGCCACCATCATCTGGACGCTGAACGGAAAGACCCTCAAGACCACCAA GTTCATCATCCTCTCCCAGGAAGGCTCACTCTGCTCCGTCTCCATCGAGAAGGCACTGCCTGAGGACAGAGGCTTATACAAGTGTGTAGCCAAGAATGAC GCTGGCCAGGCGGAGTGCTCCTGCCAAGTCACCGTGGATGATGCTCCAGCCAGTGAGAACACCAAGGCCCCAGAGATGAAATCCCGGAGGCCCAAGAGCT CTCTTCCTCCCGTGCTAGGAACTGAGAGTGATGCGACTGTGAAAAAGAAACCTGCCCCCAAGACACCTCCGAAGGCAGCAATGCCCCCTCAGATCATCCA GTTCCCTGAGGACCAGAAGGTACGCGCAGGAGAGTCAGTGGAGCTGTTTGGCAAAGTGACAGGCACTCAGCCCATCACCTGTACCTGGATGAAGTTCCGA AAGCAGATCCAGGAAAGCGAGCACATGAAGGTGGAGAACAGCGAGAATGGCAGCAAGCTCACCATCCTGGCCGCGCGCCAGGAGCACTGCGGCTGCTACA CACTGCTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAGGTCAACCTCACTGTCGTGGATAAGCCAGACCCCCCAGCTGGCACACCTTGTGCCTCTGA CATTCGGAGCTCCTCACTGACCCTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAGCATCGAGATCTGGGACTCAGCCAAC AAGACGTGGAAGGAACTAGCCACATGCCGCAGCACCTCTTTCAACGTCCAGGACCTGCTGCCTGACCACGAATATAAGTTCCGTGTACGTGCAATCAACG TGTATGGAACCAGTGAGCCAAGCCAGGAGTCTGAACTCACAACGGTAGGAGAGAAACCTGAAGAGCCGAAGGATGAAGTGGAGGTGTCAGATGATGATGA GAAGGAGCCCGAGGTTGATTACCGGACAGTGACAATCAATACTGAACAAAAAGTATCTGACTTCTACGACATTGAGGAGAGATTAGGATCTGGGAAATTT GGACAGGTCTTTCGACTTGTAGAAAAGAAAACTCGAAAAGTCTGGGCAGGGAAGTTCTTCAAGGCATATTCAGCAAAAGAGAAAGAGAATATCCGGCAGG AGATTAGCATCATGAACTGCCTCCACCACCCTAAGCTGGTCCAGTGTGTGGATGCCTTTGAAGAAAAGGCCAACATCGTCATGGTCCTGGAGATCGTGTC AGGAGGGGAGCTGTTTGAGCGCATCATTGACGAGGACTTTGAGCTGACGGAGCGTGAGTGCATCAAGTACATGCGGCAGATCTCGGAGGGAGTGGAGTAC ATCCACAAGCAGGGCATCGTGCACCTGGACCTCAAGCCGGAGAACATCATGTGTGTCAACAAGACGGGCACCAGGATCAAGCTCATCGACTTTGGTCTGG CCAGGAGGCTGGAGAATGCGGGGTCTCTGAAGGTCCTCTTTGGCACCCCAGAATTTGTGGCTCCTGAAGTGATCAACTATGAGCCCATCGGCTACGCCAC AGACATGTGGAGCATCGGGGTCATCTGCTACATCCTAGTCAGTGGCCTTTCCCCCTTCATGGGAGACAACGATAACGAAACCTTGGCCAACGTTACCTCA GCCACCTGGGACTTCGACGACGAGGCATTCGATGAGATCTCCGACGATGCCAAGGATTTCATCAGCAATCTGCTGAAGAAAGATATGAAAAACCGCCTGG ACTGCACGCAGTGCCTTCAGCATCCATGGCTAATGAAAGATACCAAGAACATGGAGGCCAAGAAACTCTCCAAGGACCGGATGAAGAAGTACATGGCAAG AAGGAAATGGCAGAAAACGGGCAATGCTGTGAGAGCCATTGGAAGACTGTCCTCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCTCAACAGGG TCACCAACCAGCCCGCTCAATGCAGAAAAACTAGAATCTGAAGAAGATGTGTCCCAAGCTTTCCTTGAGGCTGTTGCTGAGGAAAAGCCTCATGTAAAAC CCTATTTCTCTAAGACCATTCGCGATTTAGAAGTTGTGGAGGGAAGTGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCCGAGGTTGTCTG GTTCAAAGATGACCAGTCAATCAGGGAGTCCCGCCACTTCCAGATAGACTACGATGAGGACGGGAACTGCTCTTTAATTATTAGTGATGTTTGCGGGGAT GACGATGCCAAGTACACCTGCAAGGCTGTCAACAGTCTTGGAGAAGCCACCTGCACAGCAGAGCTCATTGTGGAAACGATGGAGGAAGGTGAAGGGGAAG GGGAAGAGGAAGAAGAGTGAAACAAAGCCAGAGAAAAGCAGTTTCTAAGTCATATTAAAAGGACTATTTCTCTAAAACTCAAAAAAAAAAAAAAAACTCA AGATAGTAAAAGCACCTAGTGTGATAGATTATCGGTTAGGTCATTTGTGGGTTGATTCTTCAGAAACAGCAGTTGATACCTAGCAGCGTTATTGATGGGC ATTAATCTATGTTAGTTGGCACCTTAAGATACTAGTGCAGCTAGATTTCATTTAGGGAAATCACCAGTAACTTGACTGACCAATTGATTTTAGAGAGAAA GTAACCAAACCAAATATTTATCTGGGCAAAGTCATAAATTCTCCACTTGAATGCGCTCATGAAAAATAAGGCCAAAACAAGAGTTCTGGGCCACAGCTCA GCCCAGAGGGTTCCTGGGGATGGGAGGCCTCTCTCTCCCCACCCCCTGACTCTAGAGAACTGGGTTTTCTCCCAGTACTCCAGCAATTCATTTCTGAAAG CAGTTGAGCCACTTTATTCCAAAGTACACTGCAGATGTTCAAACTCTCCATTTCTCTTTCCCCTTCCACCTGCCAGTTTTGCTGACTCTCAACTTGTCAT GAGTGTAAGCATTAAGGACATTATGCTTCTTCGATTCTGAAGACAGGTCCCTGCTCATGGATGACTCTGGCTTCCTTAGGAAAATATTTTTCTTCCAAAA TCAGTAGGAAATCTAAACTTATCCCCTCTTTGCAGATGTCTAGCAGCTTCAGACATTTGGTTAAGAACCCATGGGAAAAAAAAAATCCTTGCTAATGTGG TTTCCTTTGTAAACCAGGATTCTTATTTGTGCTGTTATAGAATATCAGCTCTGAACGTGTGGTAAAGATTTTTGTGTTTGAATATAGGAGAAATCAGTTT GCTGAAAAGTTAGTCTTAATTATCTATTGGCCACGATGAAACAGATTTCAACTGATAAAGAGCTGGAGAACTCCATGTACTTTGGAATCTCCTCCAAGAT AGCCAGAGTTTAATACATCTTCATTCTCAACACTCTCCAAAGAACTTGACCTACCTTATGGGTTCCATATTTTTCTTCTTAAATGTGCATCAATCATGCC TTGCCCCCAACCTTTAAATATATTCTTAGACCTGGTAAATGCACTCAGACTTGCGTCTTTAGGAATTTTTAACTTTCTTTCACTACATTGGCACTTAAAT TTTTTCTTTATAAAGCTTTTTGAAGGTCATAAACAAAGACCATAATTGATGATAGACCTAATACATTTCCTCTGTGTGTGTGTGTAACATTCCAAATACT TTTTTTTTCTTTTCCACTGTTTGTAAGGTGCAACAATTTAATATTTTTAAGGGACTTTTTAAGAGTTCCTTAAGAACCAATTTAAAATTACTTCAGTGCA ATCCTACACAGTATCAACATTAGAATTTTGATATTAGTCTTATGTTATCTTCCATTCTATTTTTATCTGCTTTTTGCTGCTAGTTTCAAACTGCCAGTAT TTTTCCTTTTGCTTTTAAAATAGTTACAATATTTTTCATGATAGCCACAGTATTGCCACAGTTTATTATAATAAAGGGTTTTTATTTGATTTAGCGCATT CAAAGCTTTTTTCTATCACTTTTGTGTTCAGAATATAACCTTTGTGTGCGTGTATGTTGTGTGTGTGCATGTGTGGCGTATATGTGTGTTACAGGTTAAT GCCTTCTTGGAATTGTGTTAATGTTCTCTTGGTTTATTATGCCATCAGAATGGTAAATGAGAACACTACAACTGTAGTCAGCTCACAATTTTTAAATAAA GGATACCACAGTGCATGCTGTTTGTTCAA

refseq_MYLK.F4 (forward primer)
refseq_MYLK.R4 (reverse primer)
Single exon skipping

Frame 0

GAERARSPRCAPAAGAPRRAPVPRLPGRLRRAG RILQN*QKPIRAS*REKIKVAPGCLCCSSEQLQE LPSRELQDAFPVPLAQLPQQTTEKTVTMGDVKL VASSHISKTSLSVDPSRVDSMPLTEAPAFILPP RNLCIKEGATAKFEGRVRGYPEPQVTWHRNGQPI TSGGRFLLDCGIRGTFSLVIHAVHEEDRGKYTC EATNGSGARQVTVELTVEGSFAKQLGQPVVSKT LGDRFSAPAVETRPSIWGECPPKFATKLGRVVVK EGQMGRFSCKITGRPQPQVTWLKGNVPLQPSAR VSVSEKNGMQVLEIHGVNQDDVGVYTCLVVNGS GKASMSAELSIQGLDSANRSFVRETKATNSDVRK EVTNVISKESKLDSLEAAAKSKNCSSPQRGGSP PWAANSQPQPPRESKLESCKDSPRTAPQTPVLQ KTSSSITLQAARVQPEPRAPGLGVLSPSGEERKR PAPPRPATFPTRQPGLGSQDVVSKAANRRIPME GQRDSAFPKFESKPQSQEVKENQTVKFRCEGLA VMEVAPSFSSVLKDCAVIEGQDFVLQCSVRGTPV PRITWLLNGQPIQYARSTCEAGVAELHIQDALP EDHGTYTCLAENALGQVSCSAWVTVHEKKSSRK SEYLLPVAPSKPTAPIFLQGLSDLKVMDGSQVTM TVQVSGNPPPEVIWLHNGNEIQESEDFHFEQRG TQHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQA VLTVQEPHDGTQPWFISKPRSVTASLGQSVLISC AIAGDPFPTVHWLRDGKALCKDTGHFEVLQNED VFTLVLKKVQPWHAGQYEILLKNRVGECSCQVS LMLQNSSARALPRGREPASCEDLCGGGVGADGGG SDRYGSLRPGWPARGQGWLEEEDGEDVRGVLKR RVETRQHTEEAIRQQEVEQLDFRDLLGKKVSTK TLSEDDLKEIPAEQMDFRANLQRQVKPKTVSEEE RKVHSPQQVDFRSVLAKKGTSKTPVPEKVPPPK PATPDFRSVLGGKKKLPAENGSSSAETLNAKAV ESSKPLSNAQPSGPLKPVGNAKPAETLKPMGNAK PAETLKPMGNAKPDENLKSASKEELKKDVKNDV NCKRGHAGTTDNEKRSESQGTAPAFKQKLQDVH VAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTK FIILSQEGSLCSVSIEKALPEDRGLYKCVAKND AGQAECSCQVTVDDAPASENTKAPEMKSRRPKS SLPPVLGTESDATVKKKPAPKTPPKAAMPPQIIQ FPEDQKVRAGESVELFGKVTGTQPITCTWMKFR KQIQESEHMKVENSENGSKLTILAARQEHCGCY TLLVENKLGSRQAQVNLTVVDKPDPPAGTPCASD IRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSAN KTWKELATCRSTSFNVQDLLPDHEYKFRVRAIN VYGTSEPSQESELTTVGEKPEEPKDEVEVSDDDE KEPEVDYRTVTINTEQKVSDFYDIEERLGSGKF GQVFRLVEKKTRKVWAGKFFKAYSAKEKENIRQ EISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVS GGELFERIIDEDFELTERECIKYMRQISEGVEY IHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGL ARRLENAGSLKVLFGTPEFVAPEVINYEPIGYAT DMWSIGVICYILVSGLSPFMGDNDNETLANVTS ATWDFDDEAFDEISDDAKDFISNLLKKDMKNRL DCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMAR RKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTG SPTSPLNAEKLESEEDVSQAFLEAVAEEKPHVK PYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVW FKDDQSIRESRHFQIDYDEDGNCSLIISDVCGD DDAKYTCKAVNSLGEATCTAELIVETMEEGEGE GEEEEE*NKAREKQFLSHIKRTISLKLKKKKKTQ DSKST*CDRLSVRSFVG*FFRNSS*YLAALLMG INLC*LAP*DTSAARFHLGKSPVT*LTN*F*RE SNQTKYLSGQSHKFST*MRS*KIRPKQEFWATAQ PRGFLGMGGLSLPTP*L*RTGFSPSTPAIHF*K QLSHFIPKYTADVQTLHFSFPFHLPVLLTLNLS *V*ALRTLCFFDSEDRSLLMDDSGFLRKIFFFQN Q*EI*TYPLFADV*QLQTFG*EPMGKKKSLLMW FPL*TRILICAVIEYQL*TCGKDFCV*I*EKSV C*KVSLNYLLATMKQISTDKELENSMYFGISSKI ARV*YIFILNTLQRT*PTLWVPYFSS*MCINHA LPPTFKYILRPGKCTQTCVFRNF*LSFTTLALK FFLYKAF*RS*TKTIIDDRPNTFPLCVCVTFQIL FFSFPLFVRCNNLIFLRDFLRVP*EPI*NYFSA ILHSINIRILILVLCYLPFYFYLLFAASFKLPV FFLLLLK*LQYFS**PQYCHSLL**RVFI*FSAF KAFFYHFCVQNITFVCVYVVCVHVWRICVLQVN AFLELC*CSLGLLCHQNGK*EHYNCSQLTIFK* RIPQCMLFVQ

Frame 1

ALSELGARAVRLRPGRPAERRCPGSRAAFAARE GFFKINRNQFGPAEEKK*RWLPAASAAVQSNFR SFPAESFRTPFLSHWPSCHNKQQRRR*PWGM*SW LPRHTFPKPPSVWIPQELTPCP*QRPLLSFCPL GTSASKKEPPPSSKGGSGVTQSPR*HGTETGNP SPAGAASCWIAASGGLSAL*FMLSMRRTGESIPV KPPMAVVLAR*QWS*Q*KEVLRSSLVSLLFPKP *GIDFQLQQWRPVLASGGSAHQSLLPSWAELWS KKDRWDDSPARSLAGPNRRSPGSREMFHCSRVPV CLCLRRTACRFWKSMESTKMTWECTRAWW*TGR GRPRCQLNFPSKVWTVPIGHL*EKQKPPIQMSG KR*PM*SQRSRSWTVWRLQPKARTAPAPREVAPH PGLQTASLSPQGSPSWSHARTRPERPRRPRSFR RLPAPSPCRPQEFSRNQEHQAWGSYHLLEKRGR GQLLPVQPPSPPGSLAWGAKML*ARLLTGESPWR ARGIQHSPNLRASPKARRSRKIKLSSSDVKGLP *WRWPPPSPVS*RTALLLRARILCCSAPYGGPQ CPGSLGC*MGSPSSTLAPPARPAWLSSTSRMPCR RTMAPTPA*LRMPWGRCPAAPGSPSMKRRVAGR VSTFCLWLPASPLHPSSCRASLISKSWMEARSL *LSKCQGIHPLKSSGCTMGMRSKSQRTSTLNREE LSTAFVSRKCSRRTRARTPARPGTALERSAPRP CSRYKSLTMAPSPGSSVSLAQ*QPPWARVSSSP AP*LVTPFLPCTGSEMAKPSAKTLATSRCFRMRT CSPWF*RRCSPGMPASMRSCSRTGLANAVARCH *CYRTALPEPFHGGGSLPAARTSVVEELVLMVV VVTAMGP*GLAGQQEGRVG*RRKTARTCEGC*RG AWRRGSTLRRRSASRRWSSWTSETSWGRR*VQR PYRKTT*RRSQPSRWISVPTCSGK*SQRLCLRK RGRCTAPSRSIFALSWPRRGLPRPPCLRRCHRQN LPPRIFAQCWVARRNYQQRMAAAVPRP*MPRQW RVPSP*AMHSLQGP*NPWATPSLLRP*SQWATP SLPRP*SPWAMPSLMRT*NPLAKKNSRKTLRMM* TAREAMQGPQIMKRDQRARGQPQPSSRSCKMFM WQRARSCCSSARCLLTPQPPSSGR*TERPSRPP SSSSSPRKAHSAPSPSRRHCLRTEAYTSV*PRMT LARRSAPAKSPWMMLQPVRTPRPQR*NPGGPRA LFLPC*ELRVMRL*KRNLPPRHLRRQQCPLRSS SSLRTRRYAQESQWSCLAK*QALSPSPVPG*SSE SRSRKAST*RWRTARMAASSPSWPRARSTAAAT HCWWRTSWAAGRPRSTSLSWISQTPQLAHLVPL TFGAPH*PCPGMAPHMMGAVLYSPTASRSGTQPT RRGRN*PHAAAPLSTSRTCCLTTNISSVYVQST CMEPVSQARSLNSQR*ERNLKSRRMKWRCQMMM RRSPRLITGQ*QSILNKKYLTSTTLRRD*DLGNL DRSFDL*KRKLEKSGQGSSSRHIQQKRKRISGR RLAS*TASTTLSWSSVWMPLKKRPTSSWSWRSC QEGSCLSASLTRTLS*RSVSASSTCGRSRREWST STSRASCTWTSSRRTSCVSTRRAPGSSSSTLVW PGGWRMRGL*RSSLAPQNLWLLK*STMSPSATP QTCGASGSSATS*SVAFPPSWETTITKPWPTLPQ PPGTSTTRHSMRSPTMPRISSAIC*RKI*KTAW TARSAFSIHG**KIPRTWRPRNSPRTG*RSTWQ EGNGRKRAML*EPLEDCPLWQ*SQGSVAGNPQQG HQPARSMQKN*NLKKMCPKLSLRLLLRKSLM*N PISLRPFAI*KLWREVLLDLTARLKDTQTPRLS GSKMTSQSGSPATSR*TTMRTGTAL*LLVMFAGM TMPSTPARLSTVLEKPPAQQSSLWKRWRKVKGK GKRKKSETKPEKSSF*VILKGLFL*NSKKKKKL KIVKAPSVIDYRLGHLWVDSSETAVDT*QRY*WA LIYVSWHLKILVQLDFI*GNHQ*LD*PIDFREK VTKPNIYLGKVINSPLECAHEK*GQNKSSGPQL SPEGSWGWEASLSPPPDSRELGFLPVLQQFISES S*ATLFQSTLQMFKLSISLSPSTCQFC*LSTCH ECKH*GHYASSILKTGPCSWMTLASLGKYFSSK ISRKSKLIPSLQMSSSFRHLVKNPWEKKNPC*CG FLCKPGFLFVLL*NISSERVVKIFVFEYRRNQF AEKLVLIIYWPR*NRFQLIKSWRTPCTLESPPR *PEFNTSSFSTLSKELDLPYGFHIFLLKCASIMP CPQPLNIFLDLVNALRLASLGIFNFLSLHWHLN FFFIKLFEGHKQRP*LMIDLIHFLCVCV*HSKY FFFLFHCL*GATI*YF*GTF*EFLKNQFKITSVQ SYTVSTLEF*Y*SYVIFHSIFICFLLLVSNCQY FSFCF*NSYNIFHDSHSIATVYYNKGFLFDLAH SKLFSITFVFRI*PLCACMLCVCMCGVYVCYRLM PSWNCVNVLLVYYAIRMVNENTTTVVSSQFLNK GYHSACCLF

Frame 2

R*ASSEPALCACGRGAPPSAGAPAPGPPSPRGK DSSKLTETNSGQLKRKNKGGSRLPLLQFRATSG ASQPRASGRLSCPTGPVATTNNREDGDHGGCEA GCLVTHFQNLPQCGSLKS*LHAPDRGPCFHFAPS EPLHQRRSHRQVRRAGPGLPRAPGDMAQKRATH HQRGPLPAGLRHPGDFQPCDSCCP*GGQGKVYL *SHQWQWCSPGDSGVDSRRKFCEAAWSACCFQNL RG*IFSSSSGDPS*HLGGVPTKVCYQAGPSCGQ RRTDGTILLQDHWPAPTAGHLAQGKCSTAAECP CVCV*EERHAGSGNPWSQPR*RGSVHVPGGERVG EGLDVS*TFHPRFGQCQ*VICERNKSHQFRCQE RGDQCNLKGVEAGQSGGCSQKQELLQPPERWLP TLGCKQPASAPKGVQAGVMQGLAQNGPADPGPSE DFQLHHPAGRKSSAGTKSTRPGGPITFWRREEE ASSSPSSHLPHQAAWPGEPRCCEQGC*QENPHG GPEGFSIPQI*EQAPKPGGQGKSNCQVQM*RACR DGGGPLLLQCPEGLRCY*GPGFCAAVLRTGDPS APDHLAAEWAAHPVRSLHLRGRRG*APHPGCPA GGPWHLHLPS*ECLGAGVLQRLGHRP*KEE*QEE *VPSACGSQQAHCTHLPAGPL*SQSHGWKPGHY DCPSVRESTP*SHLAAQWE*DPRVRGLPL*TER NSAQPLYPGSVPGGHGHVHLRGLEQRWRGPHPGR AHGTRASRWHPALVHQ*ASLSDSLPGPECPHLL RHSW*PLSYRALAQRWQSPLQRHWPLRGASE*G RVHPGSKEGAALACRPV*DPAQEPGWRMQLPGVT DATEQLCQSPSTGEGACQLRGPLWWRSWC*WWW **PLWVPEAWLASKRAGLARGGRRRGRARGAEE ARGDEAAH*GGDPPAGGGAAGLPRPPGEEGEYKD PIGRRPEGDPSRADGFPCQPAAASEAKDCV*GR EEGAQPPAGRFSLCPGQEGDFQDPRA*EGATAK TCHPGFSLSAGWQEEITSREWQQQCRDPECQGSG EFQAPEQCTAFRALETRGQRQAC*DPEANGQRQ ACRDPEAHGQCQA**EPEIR*QRRTQERR*E*C ELQERPCRDHR**KEIREPGDSPSLQAEAARCSC GRGQEAAAPVPGVF*PPSHHHLDAERKDPQDHQ VHHPLPGRLTLLRLHREGTA*GQRLIQVCSQE* RWPGGVLLPSHRG*CSSQ*EHQGPRDEIPEAQEL SSSRARN*E*CDCEKETCPQDTSEGSNAPSDHP VP*GPEGTRRRVSGAVWQSDRHSAHHLYLDEVP KADPGKRAHEGGEQREWQQAHHPGRAPGALRLLH TAGGEQAGQQAGPGQPHCRG*ARPPSWHTLCL* HSELLTDPVLVWLLI*WGQCCTVLQHRDLGLSQ QDVEGTSHMPQHLFQRPGPAA*PRI*VPCTCNQR VWNQ*AKPGV*THNGRRET*RAEG*SGGVR*** EGARG*LPDSDNQY*TKSI*LLRH*GEIRIWEI WTGLSTCRKENSKSLGREVLQGIFSKREREYPAG D*HHELPPPP*AGPVCGCL*RKGQHRHGPGDRV RRGAV*AHH*RGL*ADGA*VHQVHAADLGGSGV HPQAGHRAPGPQAGEHHVCQQDGHQDQAHRLWSG QEAGECGVSEGPLWHPRICGS*SDQL*AHRLRH RHVEHRGHLLHPSQWPFPLHGRQR*RNLGQRYL SHLGLRRRGIR*DLRRCQGFHQQSAEERYEKPPG LHAVPSASMANERYQEHGGQETLQGPDEEVHGK KEMAENGQCCESHWKTVLYGNDLRAQWQEILNR VTNQPAQCRKTRI*RRCVPSFP*GCC*GKASCKT LFL*DHSRFRSCGGKCC*I*LQD*RIPRPRGCL VQR*PVNQGVPPLPDRLR*GRELLFNY**CLRG *RCQVHLQGCQQSWRSHLHSRAHCGNDGGR*RGR GRGRRVKQSQRKAVSKSY*KDYFSKTQKKKKNS R**KHLV**IIG*VICGLILQKQQLIPSSVIDG H*SMLVGTLRY*CS*ISFREITSNLTDQLILERK *PNQIFIWAKS*ILHLNALMKNKAKTRVLGHSS AQRVPGDGRPLSPHPLTLENWVFSQYSSNSFLK AVEPLYSKVHCRCSNSPFLFPLPPASFADSQLVM SVSIKDIMLLRF*RQVPAHG*LWLP*ENIFLPK SVGNLNLSPLCRCLAASDIWLRTHGKKKILANV VSFVNQDSYLCCYRISALNVW*RFLCLNIGEISL LKS*S*LSIGHDETDFN**RAGELHVLWNLLQD SQSLIHLHSQHSPKNLTYLMGSIFFFLNVHQSC LAPNL*IYS*TW*MHSDLRL*EFLTFFHYIGT*I FSL*SFLKVINKDHN***T*YISSVCVCNIPNT FFFFSTVCKVQQFNIFKGLFKSSLRTNLKLLQC NPTQYQH*NFDISLMLSSILFLSAFCC*FQTASI FPFAFKIVTIFFMIATVLPQFIIIKGFYLI*RI QSFFLSLLCSEYNLCVRVCCVCACVAYMCVTG* CLLGIVLMFSWFIMPSEW*MRTLQL*SAHNF*IK DTTVHAVCS

Sequence NM_053027

GGCGCTGAGCGAGCTCGGAGCCCGCGCTGTGCGCCTGCGGCCGGGGCGCCCCGCCGAGCGCCGGTGCCCCGGCTCCCGGGCCGCCTTCGCCGCGCGGGAA GGATTCTTCAAAATTAACAGAAACCAATTCGGGCCAGCTGAAGAGAAAAAATAAAGGTGGCTCCCGGCTGCCTCTGCTGCAGTTCAGAGCAACTTCAGGA GCTTCCCAGCCGAGAGCTTCAGGACGCCTTTCCTGTCCCACTGGCCCAGTTGCCACAACAAACAACAGAGAAGACGGTGACCATGGGGGATGTGAAGCTG GTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTGGATCCCTCAAGAGTTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCTC GGAACCTCTGCATCAAAGAAGGAGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTACCCAGAGCCCCAGGTGACATGGCACAGAAACGGGCAACCCAT CACCAGCGGGGGCCGCTTCCTGCTGGATTGCGGCATCCGGGGGACTTTCAGCCTTGTGATTCATGCTGTCCATGAGGAGGACAGGGGAAAGTATACCTGT GAAGCCACCAATGGCAGTGGTGCTCGCCAGGTGACAGTGGAGTTGACAGTAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACCT TAGGGGATAGATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGCCCACCAAAGTTTGCTACCAAGCTGGGCCGAGTTGTGGTCAA AGAAGGACAGATGGGACGATTCTCCTGCAAGATCACTGGCCGGCCCCAACCGCAGGTCACCTGGCTCAAGGGAAATGTTCCACTGCAGCCGAGTGCCCGT GTGTCTGTGTCTGAGAAGAACGGCATGCAGGTTCTGGAAATCCATGGAGTCAACCAAGATGACGTGGGAGTGTACACGTGCCTGGTGGTGAACGGGTCGG GGAAGGCCTCGATGTCAGCTGAACTTTCCATCCAAGGTTTGGACAGTGCCAATAGGTCATTTGTGAGAGAAACAAAAGCCACCAATTCAGATGTCAGGAA AGAGGTGACCAATGTAATCTCAAAGGAGTCGAAGCTGGACAGTCTGGAGGCTGCAGCCAAAAGCAAGAACTGCTCCAGCCCCCAGAGAGGTGGCTCCCCA CCCTGGGCTGCAAACAGCCAGCCTCAGCCCCCAAGGGAGTCCAAGCTGGAGTCATGCAAGGACTCGCCCAGAACGGCCCCGCAGACCCCGGTCCTTCAGA AGACTTCCAGCTCCATCACCCTGCAGGCCGCAAGAGTTCAGCCGGAACCAAGAGCACCAGGCCTGGGGGTCCTATCACCTTCTGGAGAAGAGAGGAAGAG GCCAGCTCCTCCCCGTCCAGCCACCTTCCCCACCAGGCAGCCTGGCCTGGGGAGCCAAGATGTTGTGAGCAAGGCTGCTAACAGGAGAATCCCCATGGAG GGCCAGAGGGATTCAGCATTCCCCAAATTTGAGAGCAAGCCCCAAAGCCAGGAGGTCAAGGAAAATCAAACTGTCAAGTTCAGATGTGAAGTTTCCGGGA TTCCAAAGCCTGAAGTGGCCTGGTTCCTGGAAGGCACCCCCGTGAGGAGACAGGAAGGCAGCATTGAGGTTTATGAAGATGCTGGCTCCCATTACCTCTG CCTGCTGAAAGCCCGGACCAGGGACAGTGGGACATACAGCTGCACTGCTTCCAACGCCCAAGGCCAGCTGTCCTGTAGCTGGACCCTCCAAGTGGAAAGG CTTGCCGTGATGGAGGTGGCCCCCTCCTTCTCCAGTGTCCTGAAGGACTGCGCTGTTATTGAGGGCCAGGATTTTGTGCTGCAGTGCTCCGTACGGGGGA CCCCAGTGCCCCGGATCACTTGGCTGCTGAATGGGCAGCCCATCCAGTACGCTCGCTCCACCTGCGAGGCCGGCGTGGCTGAGCTCCACATCCAGGATGC CCTGCCGGAGGACCATGGCACCTACACCTGCCTAGCTGAGAATGCCTTGGGGCAGGTGTCCTGCAGCGCCTGGGTCACCGTCCATGAAAAGAAGAGTAGC AGGAAGAGTGAGTACCTTCTGCCTGTGGCTCCCAGCAAGCCCACTGCACCCATCTTCCTGCAGGGCCTCTCTGATCTCAAAGTCATGGATGGAAGCCAGG TCACTATGACTGTCCAAGTGTCAGGGAATCCACCCCCTGAAGTCATCTGGCTGCACAATGGGAATGAGATCCAAGAGTCAGAGGACTTCCACTTTGAACA GAGAGGAACTCAGCACAGCCTTTGTATCCAGGAAGTGTTCCCGGAGGACACGGGCACGTACACCTGCGAGGCCTGGAACAGCGCTGGAGAGGTCCGCACC CAGGCCGTGCTCACGGTACAAGAGCCTCACGATGGCACCCAGCCCTGGTTCATCAGTAAGCCTCGCTCAGTGACAGCCTCCCTGGGCCAGAGTGTCCTCA TCTCCTGCGCCATAGCTGGTGACCCCTTTCCTACCGTGCACTGGCTCAGAGATGGCAAAGCCCTCTGCAAAGACACTGGCCACTTCGAGGTGCTTCAGAA TGAGGACGTGTTCACCCTGGTTCTAAAGAAGGTGCAGCCCTGGCATGCCGGCCAGTATGAGATCCTGCTCAAGAACCGGGTTGGCGAATGCAGTTGCCAG GTGTCACTGATGCTACAGAACAGCTCTGCCAGAGCCCTTCCACGGGGGAGGGAGCCTGCCAGCTGCGAGGACCTCTGTGGTGGAGGAGTTGGTGCTGATG GTGGTGGTAGTGACCGCTATGGGTCCCTGAGGCCTGGCTGGCCAGCAAGAGGGCAGGGTTGGCTAGAGGAGGAAGACGGCGAGGACGTGCGAGGGGTGCT GAAGAGGCGCGTGGAGACGAGGCAGCACACTGAGGAGGCGATCCGCCAGCAGGAGGTGGAGCAGCTGGACTTCCGAGACCTCCTGGGGAAGAAGGTGAGT ACAAAGACCCTATCGGAAGACGACCTGAAGGAGATCCCAGCCGAGCAGATGGATTTCCGTGCCAACCTGCAGCGGCAAGTGAAGCCAAAGACTGTGTCTG AGGAAGAGAGGAAGGTGCACAGCCCCCAGCAGGTCGATTTTCGCTCTGTCCTGGCCAAGAAGGGGACTTCCAAGACCCCCGTGCCTGAGAAGGTGCCACC GCCAAAACCTGCCACCCCGGATTTTCGCTCAGTGCTGGGTGGCAAGAAGAAATTACCAGCAGAGAATGGCAGCAGCAGTGCCGAGACCCTGAATGCCAAG GCAGTGGAGAGTTCCAAGCCCCTGAGCAATGCACAGCCTTCAGGGCCCTTGAAACCCGTGGGCAACGCCAAGCCTGCTGAGACCCTGAAGCCAATGGGCA ACGCCAAGCCTGCCGAGACCCTGAAGCCCATGGGCAATGCCAAGCCTGATGAGAACCTGAAATCCGCTAGCAAAGAAGAACTCAAGAAAGACGTTAAGAA TGATGTGAACTGCAAGAGAGGCCATGCAGGGACCACAGATAATGAAAAGAGATCAGAGAGCCAGGGGACAGCCCCAGCCTTCAAGCAGAAGCTGCAAGAT GTTCATGTGGCAGAGGGCAAGAAGCTGCTGCTCCAGTGCCAGGTGTCTTCTGACCCCCCAGCCACCATCATCTGGACGCTGAACGGAAAGACCCTCAAGA CCACCAAGTTCATCATCCTCTCCCAGGAAGGCTCACTCTGCTCCGTCTCCATCGAGAAGGCACTGCCTGAGGACAGAGGCTTATACAAGTGTGTAGCCAA GAATGACGCTGGCCAGGCGGAGTGCTCCTGCCAAGTCACCGTGGATGATGCTCCAGCCAGTGAGAACACCAAGGCCCCAGAGATGAAATCCCGGAGGCCC AAGAGCTCTCTTCCTCCCGTGCTAGGAACTGAGAGTGATGCGACTGTGAAAAAGAAACCTGCCCCCAAGACACCTCCGAAGGCAGCAATGCCCCCTCAGA TCATCCAGTTCCCTGAGGACCAGAAGGTACGCGCAGGAGAGTCAGTGGAGCTGTTTGGCAAAGTGACAGGCACTCAGCCCATCACCTGTACCTGGATGAA GTTCCGAAAGCAGATCCAGGAAAGCGAGCACATGAAGGTGGAGAACAGCGAGAATGGCAGCAAGCTCACCATCCTGGCCGCGCGCCAGGAGCACTGCGGC TGCTACACACTGCTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAGGTCAACCTCACTGTCGTGGATAAGCCAGACCCCCCAGCTGGCACACCTTGTG CCTCTGACATTCGGAGCTCCTCACTGACCCTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAGCATCGAGATCTGGGACTC AGCCAACAAGACGTGGAAGGAACTAGCCACATGCCGCAGCACCTCTTTCAACGTCCAGGACCTGCTGCCTGACCACGAATATAAGTTCCGTGTACGTGCA ATCAACGTGTATGGAACCAGTGAGCCAAGCCAGGAGTCTGAACTCACAACGGTAGGAGAGAAACCTGAAGAGCCGAAGGATGAAGTGGAGGTGTCAGATG ATGATGAGAAGGAGCCCGAGGTTGATTACCGGACAGTGACAATCAATACTGAACAAAAAGTATCTGACTTCTACGACATTGAGGAGAGATTAGGATCTGG GAAATTTGGACAGGTCTTTCGACTTGTAGAAAAGAAAACTCGAAAAGTCTGGGCAGGGAAGTTCTTCAAGGCATATTCAGCAAAAGAGAAAGAGAATATC CGGCAGGAGATTAGCATCATGAACTGCCTCCACCACCCTAAGCTGGTCCAGTGTGTGGATGCCTTTGAAGAAAAGGCCAACATCGTCATGGTCCTGGAGA TCGTGTCAGGAGGGGAGCTGTTTGAGCGCATCATTGACGAGGACTTTGAGCTGACGGAGCGTGAGTGCATCAAGTACATGCGGCAGATCTCGGAGGGAGT GGAGTACATCCACAAGCAGGGCATCGTGCACCTGGACCTCAAGCCGGAGAACATCATGTGTGTCAACAAGACGGGCACCAGGATCAAGCTCATCGACTTT GGTCTGGCCAGGAGGCTGGAGAATGCGGGGTCTCTGAAGGTCCTCTTTGGCACCCCAGAATTTGTGGCTCCTGAAGTGATCAACTATGAGCCCATCGGCT ACGCCACAGACATGTGGAGCATCGGGGTCATCTGCTACATCCTAAACCGCCTGGACTGCACGCAGTGCCTTCAGCATCCATGGCTAATGAAAGATACCAA GAACATGGAGGCCAAGAAACTCTCCAAGGACCGGATGAAGAAGTACATGGCAAGAAGGAAATGGCAGAAAACGGGCAATGCTGTGAGAGCCATTGGAAGA CTGTCCTCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCTCAACAGGGTCACCAACCAGCCCGCTCAATGCAGAAAAACTAGAATCTGAAGAAG ATGTGTCCCAAGCTTTCCTTGAGGCTGTTGCTGAGGAAAAGCCTCATGTAAAACCCTATTTCTCTAAGACCATTCGCGATTTAGAAGTTGTGGAGGGAAG TGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCCGAGGTTGTCTGGTTCAAAGATGACCAGTCAATCAGGGAGTCCCGCCACTTCCAGATA GACTACGATGAGGACGGGAACTGCTCTTTAATTATTAGTGATGTTTGCGGGGATGACGATGCCAAGTACACCTGCAAGGCTGTCAACAGTCTTGGAGAAG CCACCTGCACAGCAGAGCTCATTGTGGAAACGATGGAGGAAGGTGAAGGGGAAGGGGAAGAGGAAGAAGAGTGAAACAAAGCCAGAGAAAAGCAGTTTCT AAGTCATATTAAAAGGACTATTTCTCTAAAACTCAAAAAAAAAAAAAAAACTCAAGATAGTAAAAGCACCTAGTGTGATAGATTATCGGTTAGGTCATTT GTGGGTTGATTCTTCAGAAACAGCAGTTGATACCTAGCAGCGTTATTGATGGGCATTAATCTATGTTAGTTGGCACCTTAAGATACTAGTGCAGCTAGAT TTCATTTAGGGAAATCACCAGTAACTTGACTGACCAATTGATTTTAGAGAGAAAGTAACCAAACCAAATATTTATCTGGGCAAAGTCATAAATTCTCCAC TTGAATGCGCTCATGAAAAATAAGGCCAAAACAAGAGTTCTGGGCCACAGCTCAGCCCAGAGGGTTCCTGGGGATGGGAGGCCTCTCTCTCCCCACCCCC TGACTCTAGAGAACTGGGTTTTCTCCCAGTACTCCAGCAATTCATTTCTGAAAGCAGTTGAGCCACTTTATTCCAAAGTACACTGCAGATGTTCAAACTC TCCATTTCTCTTTCCCCTTCCACCTGCCAGTTTTGCTGACTCTCAACTTGTCATGAGTGTAAGCATTAAGGACATTATGCTTCTTCGATTCTGAAGACAG GTCCCTGCTCATGGATGACTCTGGCTTCCTTAGGAAAATATTTTTCTTCCAAAATCAGTAGGAAATCTAAACTTATCCCCTCTTTGCAGATGTCTAGCAG CTTCAGACATTTGGTTAAGAACCCATGGGAAAAAAAAAATCCTTGCTAATGTGGTTTCCTTTGTAAACCAGGATTCTTATTTGTGCTGTTATAGAATATC AGCTCTGAACGTGTGGTAAAGATTTTTGTGTTTGAATATAGGAGAAATCAGTTTGCTGAAAAGTTAGTCTTAATTATCTATTGGCCACGATGAAACAGAT TTCAACTGATAAAGAGCTGGAGAACTCCATGTACTTTGGAATCTCCTCCAAGATAGCCAGAGTTTAATACATCTTCATTCTCAACACTCTCCAAAGAACT TGACCTACCTTATGGGTTCCATATTTTTCTTCTTAAATGTGCATCAATCATGCCTTGCCCCCAACCTTTAAATATATTCTTAGACCTGGTAAATGCACTC AGACTTGCGTCTTTAGGAATTTTTAACTTTCTTTCACTACATTGGCACTTAAATTTTTTCTTTATAAAGCTTTTTGAAGGTCATAAACAAAGACCATAAT TGATGATAGACCTAATACATTTCCTCTGTGTGTGTGTGTAACATTCCAAATACTTTTTTTTTCTTTTCCACTGTTTGTAAGGTGCAACAATTTAATATTT TTAAGGGACTTTTTAAGAGTTCCTTAAGAACCAATTTAAAATTACTTCAGTGCAATCCTACACAGTATCAACATTAGAATTTTGATATTAGTCTTATGTT ATCTTCCATTCTATTTTTATCTGCTTTTTGCTGCTAGTTTCAAACTGCCAGTATTTTTCCTTTTGCTTTTAAAATAGTTACAATATTTTTCATGATAGCC ACAGTATTGCCACAGTTTATTATAATAAAGGGTTTTTATTTGATTTAGCGCATTCAAAGCTTTTTTCTATCACTTTTGTGTTCAGAATATAACCTTTGTG TGCGTGTATGTTGTGTGTGTGCATGTGTGGCGTATATGTGTGTTACAGGTTAATGCCTTCTTGGAATTGTGTTAATGTTCTCTTGGTTTATTATGCCATC AGAATGGTAAATGAGAACACTACAACTGTAGTCAGCTCACAATTTTTAAATAAAGGATACCACAGTGCATGCTGTTTGTTCAA

refseq_MYLK.F4 (forward primer)
refseq_MYLK.R4 (reverse primer)
Single exon skipping

Frame 0

GAERARSPRCAPAAGAPRRAPVPRLPGRLRRAG RILQN*QKPIRAS*REKIKVAPGCLCCSSEQLQE LPSRELQDAFPVPLAQLPQQTTEKTVTMGDVKL VASSHISKTSLSVDPSRVDSMPLTEAPAFILPP RNLCIKEGATAKFEGRVRGYPEPQVTWHRNGQPI TSGGRFLLDCGIRGTFSLVIHAVHEEDRGKYTC EATNGSGARQVTVELTVEGSFAKQLGQPVVSKT LGDRFSAPAVETRPSIWGECPPKFATKLGRVVVK EGQMGRFSCKITGRPQPQVTWLKGNVPLQPSAR VSVSEKNGMQVLEIHGVNQDDVGVYTCLVVNGS GKASMSAELSIQGLDSANRSFVRETKATNSDVRK EVTNVISKESKLDSLEAAAKSKNCSSPQRGGSP PWAANSQPQPPRESKLESCKDSPRTAPQTPVLQ KTSSSITLQAARVQPEPRAPGLGVLSPSGEERKR PAPPRPATFPTRQPGLGSQDVVSKAANRRIPME GQRDSAFPKFESKPQSQEVKENQTVKFRCEVSG IPKPEVAWFLEGTPVRRQEGSIEVYEDAGSHYLC LLKARTRDSGTYSCTASNAQGQLSCSWTLQVER LAVMEVAPSFSSVLKDCAVIEGQDFVLQCSVRG TPVPRITWLLNGQPIQYARSTCEAGVAELHIQDA LPEDHGTYTCLAENALGQVSCSAWVTVHEKKSS RKSEYLLPVAPSKPTAPIFLQGLSDLKVMDGSQ VTMTVQVSGNPPPEVIWLHNGNEIQESEDFHFEQ RGTQHSLCIQEVFPEDTGTYTCEAWNSAGEVRT QAVLTVQEPHDGTQPWFISKPRSVTASLGQSVL ISCAIAGDPFPTVHWLRDGKALCKDTGHFEVLQN EDVFTLVLKKVQPWHAGQYEILLKNRVGECSCQ VSLMLQNSSARALPRGREPASCEDLCGGGVGAD GGGSDRYGSLRPGWPARGQGWLEEEDGEDVRGVL KRRVETRQHTEEAIRQQEVEQLDFRDLLGKKVS TKTLSEDDLKEIPAEQMDFRANLQRQVKPKTVS EEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVPP PKPATPDFRSVLGGKKKLPAENGSSSAETLNAK AVESSKPLSNAQPSGPLKPVGNAKPAETLKPMG NAKPAETLKPMGNAKPDENLKSASKEELKKDVKN DVNCKRGHAGTTDNEKRSESQGTAPAFKQKLQD VHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLK TTKFIILSQEGSLCSVSIEKALPEDRGLYKCVAK NDAGQAECSCQVTVDDAPASENTKAPEMKSRRP KSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQ IIQFPEDQKVRAGESVELFGKVTGTQPITCTWMK FRKQIQESEHMKVENSENGSKLTILAARQEHCG CYTLLVENKLGSRQAQVNLTVVDKPDPPAGTPC ASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDS ANKTWKELATCRSTSFNVQDLLPDHEYKFRVRA INVYGTSEPSQESELTTVGEKPEEPKDEVEVSD DDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSG KFGQVFRLVEKKTRKVWAGKFFKAYSAKEKENI RQEISIMNCLHHPKLVQCVDAFEEKANIVMVLE IVSGGELFERIIDEDFELTERECIKYMRQISEGV EYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDF GLARRLENAGSLKVLFGTPEFVAPEVINYEPIG YATDMWSIGVICYILNRLDCTQCLQHPWLMKDTK NMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGR LSSMAMISGLSGRKSSTGSPTSPLNAEKLESEE DVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGS AARFDCKIEGYPDPEVVWFKDDQSIRESRHFQI DYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGE ATCTAELIVETMEEGEGEGEEEEE*NKAREKQFL SHIKRTISLKLKKKKKTQDSKST*CDRLSVRSF VG*FFRNSS*YLAALLMGINLC*LAP*DTSAAR FHLGKSPVT*LTN*F*RESNQTKYLSGQSHKFST *MRS*KIRPKQEFWATAQPRGFLGMGGLSLPTP *L*RTGFSPSTPAIHF*KQLSHFIPKYTADVQT LHFSFPFHLPVLLTLNLS*V*ALRTLCFFDSEDR SLLMDDSGFLRKIFFFQNQ*EI*TYPLFADV*Q LQTFG*EPMGKKKSLLMWFPL*TRILICAVIEY QL*TCGKDFCV*I*EKSVC*KVSLNYLLATMKQI STDKELENSMYFGISSKIARV*YIFILNTLQRT *PTLWVPYFSS*MCINHALPPTFKYILRPGKCT QTCVFRNF*LSFTTLALKFFLYKAF*RS*TKTII DDRPNTFPLCVCVTFQILFFSFPLFVRCNNLIF LRDFLRVP*EPI*NYFSAILHSINIRILILVLC YLPFYFYLLFAASFKLPVFFLLLLK*LQYFS**P QYCHSLL**RVFI*FSAFKAFFYHFCVQNITFV CVYVVCVHVWRICVLQVNAFLELC*CSLGLLCH QNGK*EHYNCSQLTIFK*RIPQCMLFVQ

Frame 1

ALSELGARAVRLRPGRPAERRCPGSRAAFAARE GFFKINRNQFGPAEEKK*RWLPAASAAVQSNFR SFPAESFRTPFLSHWPSCHNKQQRRR*PWGM*SW LPRHTFPKPPSVWIPQELTPCP*QRPLLSFCPL GTSASKKEPPPSSKGGSGVTQSPR*HGTETGNP SPAGAASCWIAASGGLSAL*FMLSMRRTGESIPV KPPMAVVLAR*QWS*Q*KEVLRSSLVSLLFPKP *GIDFQLQQWRPVLASGGSAHQSLLPSWAELWS KKDRWDDSPARSLAGPNRRSPGSREMFHCSRVPV CLCLRRTACRFWKSMESTKMTWECTRAWW*TGR GRPRCQLNFPSKVWTVPIGHL*EKQKPPIQMSG KR*PM*SQRSRSWTVWRLQPKARTAPAPREVAPH PGLQTASLSPQGSPSWSHARTRPERPRRPRSFR RLPAPSPCRPQEFSRNQEHQAWGSYHLLEKRGR GQLLPVQPPSPPGSLAWGAKML*ARLLTGESPWR ARGIQHSPNLRASPKARRSRKIKLSSSDVKFPG FQSLKWPGSWKAPP*GDRKAALRFMKMLAPITS AC*KPGPGTVGHTAALLPTPKASCPVAGPSKWKG LP*WRWPPPSPVS*RTALLLRARILCCSAPYGG PQCPGSLGC*MGSPSSTLAPPARPAWLSSTSRM PCRRTMAPTPA*LRMPWGRCPAAPGSPSMKRRVA GRVSTFCLWLPASPLHPSSCRASLISKSWMEAR SL*LSKCQGIHPLKSSGCTMGMRSKSQRTSTLN REELSTAFVSRKCSRRTRARTPARPGTALERSAP RPCSRYKSLTMAPSPGSSVSLAQ*QPPWARVSS SPAP*LVTPFLPCTGSEMAKPSAKTLATSRCFR MRTCSPWF*RRCSPGMPASMRSCSRTGLANAVAR CH*CYRTALPEPFHGGGSLPAARTSVVEELVLM VVVVTAMGP*GLAGQQEGRVG*RRKTARTCEGC *RGAWRRGSTLRRRSASRRWSSWTSETSWGRR*V QRPYRKTT*RRSQPSRWISVPTCSGK*SQRLCL RKRGRCTAPSRSIFALSWPRRGLPRPPCLRRCH RQNLPPRIFAQCWVARRNYQQRMAAAVPRP*MPR QWRVPSP*AMHSLQGP*NPWATPSLLRP*SQWA TPSLPRP*SPWAMPSLMRT*NPLAKKNSRKTLR MM*TAREAMQGPQIMKRDQRARGQPQPSSRSCKM FMWQRARSCCSSARCLLTPQPPSSGR*TERPSR PPSSSSSPRKAHSAPSPSRRHCLRTEAYTSV*P RMTLARRSAPAKSPWMMLQPVRTPRPQR*NPGGP RALFLPC*ELRVMRL*KRNLPPRHLRRQQCPLR SSSSLRTRRYAQESQWSCLAK*QALSPSPVPG* SSESRSRKAST*RWRTARMAASSPSWPRARSTAA ATHCWWRTSWAAGRPRSTSLSWISQTPQLAHLV PLTFGAPH*PCPGMAPHMMGAVLYSPTASRSGT QPTRRGRN*PHAAAPLSTSRTCCLTTNISSVYVQ STCMEPVSQARSLNSQR*ERNLKSRRMKWRCQM MMRRSPRLITGQ*QSILNKKYLTSTTLRRD*DL GNLDRSFDL*KRKLEKSGQGSSSRHIQQKRKRIS GRRLAS*TASTTLSWSSVWMPLKKRPTSSWSWR SCQEGSCLSASLTRTLS*RSVSASSTCGRSRRE WSTSTSRASCTWTSSRRTSCVSTRRAPGSSSSTL VWPGGWRMRGL*RSSLAPQNLWLLK*STMSPSA TPQTCGASGSSATS*TAWTARSAFSIHG**KIP RTWRPRNSPRTG*RSTWQEGNGRKRAML*EPLED CPLWQ*SQGSVAGNPQQGHQPARSMQKN*NLKK MCPKLSLRLLLRKSLM*NPISLRPFAI*KLWRE VLLDLTARLKDTQTPRLSGSKMTSQSGSPATSR* TTMRTGTAL*LLVMFAGMTMPSTPARLSTVLEK PPAQQSSLWKRWRKVKGKGKRKKSETKPEKSSF *VILKGLFL*NSKKKKKLKIVKAPSVIDYRLGHL WVDSSETAVDT*QRY*WALIYVSWHLKILVQLD FI*GNHQ*LD*PIDFREKVTKPNIYLGKVINSP LECAHEK*GQNKSSGPQLSPEGSWGWEASLSPPP DSRELGFLPVLQQFISESS*ATLFQSTLQMFKL SISLSPSTCQFC*LSTCHECKH*GHYASSILKT GPCSWMTLASLGKYFSSKISRKSKLIPSLQMSSS FRHLVKNPWEKKNPC*CGFLCKPGFLFVLL*NI SSERVVKIFVFEYRRNQFAEKLVLIIYWPR*NR FQLIKSWRTPCTLESPPR*PEFNTSSFSTLSKEL DLPYGFHIFLLKCASIMPCPQPLNIFLDLVNAL RLASLGIFNFLSLHWHLNFFFIKLFEGHKQRP* LMIDLIHFLCVCV*HSKYFFFLFHCL*GATI*YF *GTF*EFLKNQFKITSVQSYTVSTLEF*Y*SYV IFHSIFICFLLLVSNCQYFSFCF*NSYNIFHDS HSIATVYYNKGFLFDLAHSKLFSITFVFRI*PLC ACMLCVCMCGVYVCYRLMPSWNCVNVLLVYYAI RMVNENTTTVVSSQFLNKGYHSACCLF

Frame 2

R*ASSEPALCACGRGAPPSAGAPAPGPPSPRGK DSSKLTETNSGQLKRKNKGGSRLPLLQFRATSG ASQPRASGRLSCPTGPVATTNNREDGDHGGCEA GCLVTHFQNLPQCGSLKS*LHAPDRGPCFHFAPS EPLHQRRSHRQVRRAGPGLPRAPGDMAQKRATH HQRGPLPAGLRHPGDFQPCDSCCP*GGQGKVYL *SHQWQWCSPGDSGVDSRRKFCEAAWSACCFQNL RG*IFSSSSGDPS*HLGGVPTKVCYQAGPSCGQ RRTDGTILLQDHWPAPTAGHLAQGKCSTAAECP CVCV*EERHAGSGNPWSQPR*RGSVHVPGGERVG EGLDVS*TFHPRFGQCQ*VICERNKSHQFRCQE RGDQCNLKGVEAGQSGGCSQKQELLQPPERWLP TLGCKQPASAPKGVQAGVMQGLAQNGPADPGPSE DFQLHHPAGRKSSAGTKSTRPGGPITFWRREEE ASSSPSSHLPHQAAWPGEPRCCEQGC*QENPHG GPEGFSIPQI*EQAPKPGGQGKSNCQVQM*SFRD SKA*SGLVPGRHPREETGRQH*GL*RCWLPLPL PAESPDQGQWDIQLHCFQRPRPAVL*LDPPSGK ACRDGGGPLLLQCPEGLRCY*GPGFCAAVLRTGD PSAPDHLAAEWAAHPVRSLHLRGRRG*APHPGC PAGGPWHLHLPS*ECLGAGVLQRLGHRP*KEE* QEE*VPSACGSQQAHCTHLPAGPL*SQSHGWKPG HYDCPSVRESTP*SHLAAQWE*DPRVRGLPL*T ERNSAQPLYPGSVPGGHGHVHLRGLEQRWRGPH PGRAHGTRASRWHPALVHQ*ASLSDSLPGPECPH LLRHSW*PLSYRALAQRWQSPLQRHWPLRGASE *GRVHPGSKEGAALACRPV*DPAQEPGWRMQLP GVTDATEQLCQSPSTGEGACQLRGPLWWRSWC*W WW**PLWVPEAWLASKRAGLARGGRRRGRARGA EEARGDEAAH*GGDPPAGGGAAGLPRPPGEEGE YKDPIGRRPEGDPSRADGFPCQPAAASEAKDCV* GREEGAQPPAGRFSLCPGQEGDFQDPRA*EGAT AKTCHPGFSLSAGWQEEITSREWQQQCRDPECQ GSGEFQAPEQCTAFRALETRGQRQAC*DPEANGQ RQACRDPEAHGQCQA**EPEIR*QRRTQERR*E *CELQERPCRDHR**KEIREPGDSPSLQAEAAR CSCGRGQEAAAPVPGVF*PPSHHHLDAERKDPQD HQVHHPLPGRLTLLRLHREGTA*GQRLIQVCSQ E*RWPGGVLLPSHRG*CSSQ*EHQGPRDEIPEA QELSSSRARN*E*CDCEKETCPQDTSEGSNAPSD HPVP*GPEGTRRRVSGAVWQSDRHSAHHLYLDE VPKADPGKRAHEGGEQREWQQAHHPGRAPGALR LLHTAGGEQAGQQAGPGQPHCRG*ARPPSWHTLC L*HSELLTDPVLVWLLI*WGQCCTVLQHRDLGL SQQDVEGTSHMPQHLFQRPGPAA*PRI*VPCTC NQRVWNQ*AKPGV*THNGRRET*RAEG*SGGVR* **EGARG*LPDSDNQY*TKSI*LLRH*GEIRIW EIWTGLSTCRKENSKSLGREVLQGIFSKREREY PAGD*HHELPPPP*AGPVCGCL*RKGQHRHGPGD RVRRGAV*AHH*RGL*ADGA*VHQVHAADLGGS GVHPQAGHRAPGPQAGEHHVCQQDGHQDQAHRL WSGQEAGECGVSEGPLWHPRICGS*SDQL*AHRL RHRHVEHRGHLLHPKPPGLHAVPSASMANERYQ EHGGQETLQGPDEEVHGKKEMAENGQCCESHWK TVLYGNDLRAQWQEILNRVTNQPAQCRKTRI*RR CVPSFP*GCC*GKASCKTLFL*DHSRFRSCGGK CC*I*LQD*RIPRPRGCLVQR*PVNQGVPPLPD RLR*GRELLFNY**CLRG*RCQVHLQGCQQSWRS HLHSRAHCGNDGGR*RGRGRGRRVKQSQRKAVS KSY*KDYFSKTQKKKKNSR**KHLV**IIG*VI CGLILQKQQLIPSSVIDGH*SMLVGTLRY*CS*I SFREITSNLTDQLILERK*PNQIFIWAKS*ILH LNALMKNKAKTRVLGHSSAQRVPGDGRPLSPHP LTLENWVFSQYSSNSFLKAVEPLYSKVHCRCSNS PFLFPLPPASFADSQLVMSVSIKDIMLLRF*RQ VPAHG*LWLP*ENIFLPKSVGNLNLSPLCRCLA ASDIWLRTHGKKKILANVVSFVNQDSYLCCYRIS ALNVW*RFLCLNIGEISLLKS*S*LSIGHDETD FN**RAGELHVLWNLLQDSQSLIHLHSQHSPKN LTYLMGSIFFFLNVHQSCLAPNL*IYS*TW*MHS DLRL*EFLTFFHYIGT*IFSL*SFLKVINKDHN ***T*YISSVCVCNIPNTFFFFSTVCKVQQFNI FKGLFKSSLRTNLKLLQCNPTQYQH*NFDISLML SSILFLSAFCC*FQTASIFPFAFKIVTIFFMIA TVLPQFIIIKGFYLI*RIQSFFLSLLCSEYNLC VRVCCVCACVAYMCVTG*CLLGIVLMFSWFIMPS EW*MRTLQL*SAHNF*IKDTTVHAVCS

Sequence NM_053025

GGCGCTGAGCGAGCTCGGAGCCCGCGCTGTGCGCCTGCGGCCGGGGCGCCCCGCCGAGCGCCGGTGCCCCGGCTCCCGGGCCGCCTTCGCCGCGCGGGAA GGATTCTTCAAAATTAACAGAAACCAATTCGGGCCAGCTGAAGAGAAAAAATAAAGGTGGCTCCCGGCTGCCTCTGCTGCAGTTCAGAGCAACTTCAGGA GCTTCCCAGCCGAGAGCTTCAGGACGCCTTTCCTGTCCCACTGGCCCAGTTGCCACAACAAACAACAGAGAAGACGGTGACCATGGGGGATGTGAAGCTG GTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTGGATCCCTCAAGAGTTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCTC GGAACCTCTGCATCAAAGAAGGAGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTACCCAGAGCCCCAGGTGACATGGCACAGAAACGGGCAACCCAT CACCAGCGGGGGCCGCTTCCTGCTGGATTGCGGCATCCGGGGGACTTTCAGCCTTGTGATTCATGCTGTCCATGAGGAGGACAGGGGAAAGTATACCTGT GAAGCCACCAATGGCAGTGGTGCTCGCCAGGTGACAGTGGAGTTGACAGTAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACCT TAGGGGATAGATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGCCCACCAAAGTTTGCTACCAAGCTGGGCCGAGTTGTGGTCAA AGAAGGACAGATGGGACGATTCTCCTGCAAGATCACTGGCCGGCCCCAACCGCAGGTCACCTGGCTCAAGGGAAATGTTCCACTGCAGCCGAGTGCCCGT GTGTCTGTGTCTGAGAAGAACGGCATGCAGGTTCTGGAAATCCATGGAGTCAACCAAGATGACGTGGGAGTGTACACGTGCCTGGTGGTGAACGGGTCGG GGAAGGCCTCGATGTCAGCTGAACTTTCCATCCAAGGTTTGGACAGTGCCAATAGGTCATTTGTGAGAGAAACAAAAGCCACCAATTCAGATGTCAGGAA AGAGGTGACCAATGTAATCTCAAAGGAGTCGAAGCTGGACAGTCTGGAGGCTGCAGCCAAAAGCAAGAACTGCTCCAGCCCCCAGAGAGGTGGCTCCCCA CCCTGGGCTGCAAACAGCCAGCCTCAGCCCCCAAGGGAGTCCAAGCTGGAGTCATGCAAGGACTCGCCCAGAACGGCCCCGCAGACCCCGGTCCTTCAGA AGACTTCCAGCTCCATCACCCTGCAGGCCGCAAGAGTTCAGCCGGAACCAAGAGCACCAGGCCTGGGGGTCCTATCACCTTCTGGAGAAGAGAGGAAGAG GCCAGCTCCTCCCCGTCCAGCCACCTTCCCCACCAGGCAGCCTGGCCTGGGGAGCCAAGATGTTGTGAGCAAGGCTGCTAACAGGAGAATCCCCATGGAG GGCCAGAGGGATTCAGCATTCCCCAAATTTGAGAGCAAGCCCCAAAGCCAGGAGGTCAAGGAAAATCAAACTGTCAAGTTCAGATGTGAAGTTTCCGGGA TTCCAAAGCCTGAAGTGGCCTGGTTCCTGGAAGGCACCCCCGTGAGGAGACAGGAAGGCAGCATTGAGGTTTATGAAGATGCTGGCTCCCATTACCTCTG CCTGCTGAAAGCCCGGACCAGGGACAGTGGGACATACAGCTGCACTGCTTCCAACGCCCAAGGCCAGCTGTCCTGTAGCTGGACCCTCCAAGTGGAAAGG CTTGCCGTGATGGAGGTGGCCCCCTCCTTCTCCAGTGTCCTGAAGGACTGCGCTGTTATTGAGGGCCAGGATTTTGTGCTGCAGTGCTCCGTACGGGGGA CCCCAGTGCCCCGGATCACTTGGCTGCTGAATGGGCAGCCCATCCAGTACGCTCGCTCCACCTGCGAGGCCGGCGTGGCTGAGCTCCACATCCAGGATGC CCTGCCGGAGGACCATGGCACCTACACCTGCCTAGCTGAGAATGCCTTGGGGCAGGTGTCCTGCAGCGCCTGGGTCACCGTCCATGAAAAGAAGAGTAGC AGGAAGAGTGAGTACCTTCTGCCTGTGGCTCCCAGCAAGCCCACTGCACCCATCTTCCTGCAGGGCCTCTCTGATCTCAAAGTCATGGATGGAAGCCAGG TCACTATGACTGTCCAAGTGTCAGGGAATCCACCCCCTGAAGTCATCTGGCTGCACAATGGGAATGAGATCCAAGAGTCAGAGGACTTCCACTTTGAACA GAGAGGAACTCAGCACAGCCTTTGTATCCAGGAAGTGTTCCCGGAGGACACGGGCACGTACACCTGCGAGGCCTGGAACAGCGCTGGAGAGGTCCGCACC CAGGCCGTGCTCACGGTACAAGAGCCTCACGATGGCACCCAGCCCTGGTTCATCAGTAAGCCTCGCTCAGTGACAGCCTCCCTGGGCCAGAGTGTCCTCA TCTCCTGCGCCATAGCTGGTGACCCCTTTCCTACCGTGCACTGGCTCAGAGATGGCAAAGCCCTCTGCAAAGACACTGGCCACTTCGAGGTGCTTCAGAA TGAGGACGTGTTCACCCTGGTTCTAAAGAAGGTGCAGCCCTGGCATGCCGGCCAGTATGAGATCCTGCTCAAGAACCGGGTTGGCGAATGCAGTTGCCAG GTGTCACTGATGCTACAGAACAGCTCTGCCAGAGCCCTTCCACGGGGGAGGGAGCCTGCCAGCTGCGAGGACCTCTGTGGTGGAGGAGTTGGTGCTGATG GTGGTGGTAGTGACCGCTATGGGTCCCTGAGGCCTGGCTGGCCAGCAAGAGGGCAGGGTTGGCTAGAGGAGGAAGACGGCGAGGACGTGCGAGGGGTGCT GAAGAGGCGCGTGGAGACGAGGCAGCACACTGAGGAGGCGATCCGCCAGCAGGAGGTGGAGCAGCTGGACTTCCGAGACCTCCTGGGGAAGAAGGTGAGT ACAAAGACCCTATCGGAAGACGACCTGAAGGAGATCCCAGCCGAGCAGATGGATTTCCGTGCCAACCTGCAGCGGCAAGTGAAGCCAAAGACTGTGTCTG AGGAAGAGAGGAAGGTGCACAGCCCCCAGCAGGTCGATTTTCGCTCTGTCCTGGCCAAGAAGGGGACTTCCAAGACCCCCGTGCCTGAGAAGGTGCCACC GCCAAAACCTGCCACCCCGGATTTTCGCTCAGTGCTGGGTGGCAAGAAGAAATTACCAGCAGAGAATGGCAGCAGCAGTGCCGAGACCCTGAATGCCAAG GCAGTGGAGAGTTCCAAGCCCCTGAGCAATGCACAGCCTTCAGGGCCCTTGAAACCCGTGGGCAACGCCAAGCCTGCTGAGACCCTGAAGCCAATGGGCA ACGCCAAGCCTGCCGAGACCCTGAAGCCCATGGGCAATGCCAAGCCTGATGAGAACCTGAAATCCGCTAGCAAAGAAGAACTCAAGAAAGACGTTAAGAA TGATGTGAACTGCAAGAGAGGCCATGCAGGGACCACAGATAATGAAAAGAGATCAGAGAGCCAGGGGACAGCCCCAGCCTTCAAGCAGAAGCTGCAAGAT GTTCATGTGGCAGAGGGCAAGAAGCTGCTGCTCCAGTGCCAGGTGTCTTCTGACCCCCCAGCCACCATCATCTGGACGCTGAACGGAAAGACCCTCAAGA CCACCAAGTTCATCATCCTCTCCCAGGAAGGCTCACTCTGCTCCGTCTCCATCGAGAAGGCACTGCCTGAGGACAGAGGCTTATACAAGTGTGTAGCCAA GAATGACGCTGGCCAGGCGGAGTGCTCCTGCCAAGTCACCGTGGATGATGCTCCAGCCAGTGAGAACACCAAGGCCCCAGAGATGAAATCCCGGAGGCCC AAGAGCTCTCTTCCTCCCGTGCTAGGAACTGAGAGTGATGCGACTGTGAAAAAGAAACCTGCCCCCAAGACACCTCCGAAGGCAGCAATGCCCCCTCAGA TCATCCAGTTCCCTGAGGACCAGAAGGTACGCGCAGGAGAGTCAGTGGAGCTGTTTGGCAAAGTGACAGGCACTCAGCCCATCACCTGTACCTGGATGAA GTTCCGAAAGCAGATCCAGGAAAGCGAGCACATGAAGGTGGAGAACAGCGAGAATGGCAGCAAGCTCACCATCCTGGCCGCGCGCCAGGAGCACTGCGGC TGCTACACACTGCTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAGGTCAACCTCACTGTCGTGGATAAGCCAGACCCCCCAGCTGGCACACCTTGTG CCTCTGACATTCGGAGCTCCTCACTGACCCTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAGCATCGAGATCTGGGACTC AGCCAACAAGACGTGGAAGGAACTAGCCACATGCCGCAGCACCTCTTTCAACGTCCAGGACCTGCTGCCTGACCACGAATATAAGTTCCGTGTACGTGCA ATCAACGTGTATGGAACCAGTGAGCCAAGCCAGGAGTCTGAACTCACAACGGTAGGAGAGAAACCTGAAGAGCCGAAGGATGAAGTGGAGGTGTCAGATG ATGATGAGAAGGAGCCCGAGGTTGATTACCGGACAGTGACAATCAATACTGAACAAAAAGTATCTGACTTCTACGACATTGAGGAGAGATTAGGATCTGG GAAATTTGGACAGGTCTTTCGACTTGTAGAAAAGAAAACTCGAAAAGTCTGGGCAGGGAAGTTCTTCAAGGCATATTCAGCAAAAGAGAAAGAGAATATC CGGCAGGAGATTAGCATCATGAACTGCCTCCACCACCCTAAGCTGGTCCAGTGTGTGGATGCCTTTGAAGAAAAGGCCAACATCGTCATGGTCCTGGAGA TCGTGTCAGGAGGGGAGCTGTTTGAGCGCATCATTGACGAGGACTTTGAGCTGACGGAGCGTGAGTGCATCAAGTACATGCGGCAGATCTCGGAGGGAGT GGAGTACATCCACAAGCAGGGCATCGTGCACCTGGACCTCAAGCCGGAGAACATCATGTGTGTCAACAAGACGGGCACCAGGATCAAGCTCATCGACTTT GGTCTGGCCAGGAGGCTGGAGAATGCGGGGTCTCTGAAGGTCCTCTTTGGCACCCCAGAATTTGTGGCTCCTGAAGTGATCAACTATGAGCCCATCGGCT ACGCCACAGACATGTGGAGCATCGGGGTCATCTGCTACATCCTAGTCAGTGGCCTTTCCCCCTTCATGGGAGACAACGATAACGAAACCTTGGCCAACGT TACCTCAGCCACCTGGGACTTCGACGACGAGGCATTCGATGAGATCTCCGACGATGCCAAGGATTTCATCAGCAATCTGCTGAAGAAAGATATGAAAAAC CGCCTGGACTGCACGCAGTGCCTTCAGCATCCATGGCTAATGAAAGATACCAAGAACATGGAGGCCAAGAAACTCTCCAAGGACCGGATGAAGAAGTACA TGGCAAGAAGGAAATGGCAGAAAACGGGCAATGCTGTGAGAGCCATTGGAAGACTGTCCTCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCTC AACAGGGTCACCAACCAGCCCGCTCAATGCAGAAAAACTAGAATCTGAAGAAGATGTGTCCCAAGCTTTCCTTGAGGCTGTTGCTGAGGAAAAGCCTCAT GTAAAACCCTATTTCTCTAAGACCATTCGCGATTTAGAAGTTGTGGAGGGAAGTGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCCGAGG TTGTCTGGTTCAAAGATGACCAGTCAATCAGGGAGTCCCGCCACTTCCAGATAGACTACGATGAGGACGGGAACTGCTCTTTAATTATTAGTGATGTTTG CGGGGATGACGATGCCAAGTACACCTGCAAGGCTGTCAACAGTCTTGGAGAAGCCACCTGCACAGCAGAGCTCATTGTGGAAACGATGGAGGAAGGTGAA GGGGAAGGGGAAGAGGAAGAAGAGTGAAACAAAGCCAGAGAAAAGCAGTTTCTAAGTCATATTAAAAGGACTATTTCTCTAAAACTCAAAAAAAAAAAAA AAACTCAAGATAGTAAAAGCACCTAGTGTGATAGATTATCGGTTAGGTCATTTGTGGGTTGATTCTTCAGAAACAGCAGTTGATACCTAGCAGCGTTATT GATGGGCATTAATCTATGTTAGTTGGCACCTTAAGATACTAGTGCAGCTAGATTTCATTTAGGGAAATCACCAGTAACTTGACTGACCAATTGATTTTAG AGAGAAAGTAACCAAACCAAATATTTATCTGGGCAAAGTCATAAATTCTCCACTTGAATGCGCTCATGAAAAATAAGGCCAAAACAAGAGTTCTGGGCCA CAGCTCAGCCCAGAGGGTTCCTGGGGATGGGAGGCCTCTCTCTCCCCACCCCCTGACTCTAGAGAACTGGGTTTTCTCCCAGTACTCCAGCAATTCATTT CTGAAAGCAGTTGAGCCACTTTATTCCAAAGTACACTGCAGATGTTCAAACTCTCCATTTCTCTTTCCCCTTCCACCTGCCAGTTTTGCTGACTCTCAAC TTGTCATGAGTGTAAGCATTAAGGACATTATGCTTCTTCGATTCTGAAGACAGGTCCCTGCTCATGGATGACTCTGGCTTCCTTAGGAAAATATTTTTCT TCCAAAATCAGTAGGAAATCTAAACTTATCCCCTCTTTGCAGATGTCTAGCAGCTTCAGACATTTGGTTAAGAACCCATGGGAAAAAAAAAATCCTTGCT AATGTGGTTTCCTTTGTAAACCAGGATTCTTATTTGTGCTGTTATAGAATATCAGCTCTGAACGTGTGGTAAAGATTTTTGTGTTTGAATATAGGAGAAA TCAGTTTGCTGAAAAGTTAGTCTTAATTATCTATTGGCCACGATGAAACAGATTTCAACTGATAAAGAGCTGGAGAACTCCATGTACTTTGGAATCTCCT CCAAGATAGCCAGAGTTTAATACATCTTCATTCTCAACACTCTCCAAAGAACTTGACCTACCTTATGGGTTCCATATTTTTCTTCTTAAATGTGCATCAA TCATGCCTTGCCCCCAACCTTTAAATATATTCTTAGACCTGGTAAATGCACTCAGACTTGCGTCTTTAGGAATTTTTAACTTTCTTTCACTACATTGGCA CTTAAATTTTTTCTTTATAAAGCTTTTTGAAGGTCATAAACAAAGACCATAATTGATGATAGACCTAATACATTTCCTCTGTGTGTGTGTGTAACATTCC AAATACTTTTTTTTTCTTTTCCACTGTTTGTAAGGTGCAACAATTTAATATTTTTAAGGGACTTTTTAAGAGTTCCTTAAGAACCAATTTAAAATTACTT CAGTGCAATCCTACACAGTATCAACATTAGAATTTTGATATTAGTCTTATGTTATCTTCCATTCTATTTTTATCTGCTTTTTGCTGCTAGTTTCAAACTG CCAGTATTTTTCCTTTTGCTTTTAAAATAGTTACAATATTTTTCATGATAGCCACAGTATTGCCACAGTTTATTATAATAAAGGGTTTTTATTTGATTTA GCGCATTCAAAGCTTTTTTCTATCACTTTTGTGTTCAGAATATAACCTTTGTGTGCGTGTATGTTGTGTGTGTGCATGTGTGGCGTATATGTGTGTTACA GGTTAATGCCTTCTTGGAATTGTGTTAATGTTCTCTTGGTTTATTATGCCATCAGAATGGTAAATGAGAACACTACAACTGTAGTCAGCTCACAATTTTT AAATAAAGGATACCACAGTGCATGCTGTTTGTTCAA

refseq_MYLK.F4 (forward primer)
refseq_MYLK.R4 (reverse primer)
Single exon skipping

Frame 0

GAERARSPRCAPAAGAPRRAPVPRLPGRLRRAG RILQN*QKPIRAS*REKIKVAPGCLCCSSEQLQE LPSRELQDAFPVPLAQLPQQTTEKTVTMGDVKL VASSHISKTSLSVDPSRVDSMPLTEAPAFILPP RNLCIKEGATAKFEGRVRGYPEPQVTWHRNGQPI TSGGRFLLDCGIRGTFSLVIHAVHEEDRGKYTC EATNGSGARQVTVELTVEGSFAKQLGQPVVSKT LGDRFSAPAVETRPSIWGECPPKFATKLGRVVVK EGQMGRFSCKITGRPQPQVTWLKGNVPLQPSAR VSVSEKNGMQVLEIHGVNQDDVGVYTCLVVNGS GKASMSAELSIQGLDSANRSFVRETKATNSDVRK EVTNVISKESKLDSLEAAAKSKNCSSPQRGGSP PWAANSQPQPPRESKLESCKDSPRTAPQTPVLQ KTSSSITLQAARVQPEPRAPGLGVLSPSGEERKR PAPPRPATFPTRQPGLGSQDVVSKAANRRIPME GQRDSAFPKFESKPQSQEVKENQTVKFRCEVSG IPKPEVAWFLEGTPVRRQEGSIEVYEDAGSHYLC LLKARTRDSGTYSCTASNAQGQLSCSWTLQVER LAVMEVAPSFSSVLKDCAVIEGQDFVLQCSVRG TPVPRITWLLNGQPIQYARSTCEAGVAELHIQDA LPEDHGTYTCLAENALGQVSCSAWVTVHEKKSS RKSEYLLPVAPSKPTAPIFLQGLSDLKVMDGSQ VTMTVQVSGNPPPEVIWLHNGNEIQESEDFHFEQ RGTQHSLCIQEVFPEDTGTYTCEAWNSAGEVRT QAVLTVQEPHDGTQPWFISKPRSVTASLGQSVL ISCAIAGDPFPTVHWLRDGKALCKDTGHFEVLQN EDVFTLVLKKVQPWHAGQYEILLKNRVGECSCQ VSLMLQNSSARALPRGREPASCEDLCGGGVGAD GGGSDRYGSLRPGWPARGQGWLEEEDGEDVRGVL KRRVETRQHTEEAIRQQEVEQLDFRDLLGKKVS TKTLSEDDLKEIPAEQMDFRANLQRQVKPKTVS EEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVPP PKPATPDFRSVLGGKKKLPAENGSSSAETLNAK AVESSKPLSNAQPSGPLKPVGNAKPAETLKPMG NAKPAETLKPMGNAKPDENLKSASKEELKKDVKN DVNCKRGHAGTTDNEKRSESQGTAPAFKQKLQD VHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLK TTKFIILSQEGSLCSVSIEKALPEDRGLYKCVAK NDAGQAECSCQVTVDDAPASENTKAPEMKSRRP KSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQ IIQFPEDQKVRAGESVELFGKVTGTQPITCTWMK FRKQIQESEHMKVENSENGSKLTILAARQEHCG CYTLLVENKLGSRQAQVNLTVVDKPDPPAGTPC ASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDS ANKTWKELATCRSTSFNVQDLLPDHEYKFRVRA INVYGTSEPSQESELTTVGEKPEEPKDEVEVSD DDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSG KFGQVFRLVEKKTRKVWAGKFFKAYSAKEKENI RQEISIMNCLHHPKLVQCVDAFEEKANIVMVLE IVSGGELFERIIDEDFELTERECIKYMRQISEGV EYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDF GLARRLENAGSLKVLFGTPEFVAPEVINYEPIG YATDMWSIGVICYILVSGLSPFMGDNDNETLANV TSATWDFDDEAFDEISDDAKDFISNLLKKDMKN RLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKY MARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSS TGSPTSPLNAEKLESEEDVSQAFLEAVAEEKPH VKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPE VVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVC GDDDAKYTCKAVNSLGEATCTAELIVETMEEGE GEGEEEEE*NKAREKQFLSHIKRTISLKLKKKK KTQDSKST*CDRLSVRSFVG*FFRNSS*YLAALL MGINLC*LAP*DTSAARFHLGKSPVT*LTN*F* RESNQTKYLSGQSHKFST*MRS*KIRPKQEFWA TAQPRGFLGMGGLSLPTP*L*RTGFSPSTPAIHF *KQLSHFIPKYTADVQTLHFSFPFHLPVLLTLN LS*V*ALRTLCFFDSEDRSLLMDDSGFLRKIFF FQNQ*EI*TYPLFADV*QLQTFG*EPMGKKKSLL MWFPL*TRILICAVIEYQL*TCGKDFCV*I*EK SVC*KVSLNYLLATMKQISTDKELENSMYFGIS SKIARV*YIFILNTLQRT*PTLWVPYFSS*MCIN HALPPTFKYILRPGKCTQTCVFRNF*LSFTTLA LKFFLYKAF*RS*TKTIIDDRPNTFPLCVCVTF QILFFSFPLFVRCNNLIFLRDFLRVP*EPI*NYF SAILHSINIRILILVLCYLPFYFYLLFAASFKL PVFFLLLLK*LQYFS**PQYCHSLL**RVFI*F SAFKAFFYHFCVQNITFVCVYVVCVHVWRICVLQ VNAFLELC*CSLGLLCHQNGK*EHYNCSQLTIF K*RIPQCMLFVQ

Frame 1

ALSELGARAVRLRPGRPAERRCPGSRAAFAARE GFFKINRNQFGPAEEKK*RWLPAASAAVQSNFR SFPAESFRTPFLSHWPSCHNKQQRRR*PWGM*SW LPRHTFPKPPSVWIPQELTPCP*QRPLLSFCPL GTSASKKEPPPSSKGGSGVTQSPR*HGTETGNP SPAGAASCWIAASGGLSAL*FMLSMRRTGESIPV KPPMAVVLAR*QWS*Q*KEVLRSSLVSLLFPKP *GIDFQLQQWRPVLASGGSAHQSLLPSWAELWS KKDRWDDSPARSLAGPNRRSPGSREMFHCSRVPV CLCLRRTACRFWKSMESTKMTWECTRAWW*TGR GRPRCQLNFPSKVWTVPIGHL*EKQKPPIQMSG KR*PM*SQRSRSWTVWRLQPKARTAPAPREVAPH PGLQTASLSPQGSPSWSHARTRPERPRRPRSFR RLPAPSPCRPQEFSRNQEHQAWGSYHLLEKRGR GQLLPVQPPSPPGSLAWGAKML*ARLLTGESPWR ARGIQHSPNLRASPKARRSRKIKLSSSDVKFPG FQSLKWPGSWKAPP*GDRKAALRFMKMLAPITS AC*KPGPGTVGHTAALLPTPKASCPVAGPSKWKG LP*WRWPPPSPVS*RTALLLRARILCCSAPYGG PQCPGSLGC*MGSPSSTLAPPARPAWLSSTSRM PCRRTMAPTPA*LRMPWGRCPAAPGSPSMKRRVA GRVSTFCLWLPASPLHPSSCRASLISKSWMEAR SL*LSKCQGIHPLKSSGCTMGMRSKSQRTSTLN REELSTAFVSRKCSRRTRARTPARPGTALERSAP RPCSRYKSLTMAPSPGSSVSLAQ*QPPWARVSS SPAP*LVTPFLPCTGSEMAKPSAKTLATSRCFR MRTCSPWF*RRCSPGMPASMRSCSRTGLANAVAR CH*CYRTALPEPFHGGGSLPAARTSVVEELVLM VVVVTAMGP*GLAGQQEGRVG*RRKTARTCEGC *RGAWRRGSTLRRRSASRRWSSWTSETSWGRR*V QRPYRKTT*RRSQPSRWISVPTCSGK*SQRLCL RKRGRCTAPSRSIFALSWPRRGLPRPPCLRRCH RQNLPPRIFAQCWVARRNYQQRMAAAVPRP*MPR QWRVPSP*AMHSLQGP*NPWATPSLLRP*SQWA TPSLPRP*SPWAMPSLMRT*NPLAKKNSRKTLR MM*TAREAMQGPQIMKRDQRARGQPQPSSRSCKM FMWQRARSCCSSARCLLTPQPPSSGR*TERPSR PPSSSSSPRKAHSAPSPSRRHCLRTEAYTSV*P RMTLARRSAPAKSPWMMLQPVRTPRPQR*NPGGP RALFLPC*ELRVMRL*KRNLPPRHLRRQQCPLR SSSSLRTRRYAQESQWSCLAK*QALSPSPVPG* SSESRSRKAST*RWRTARMAASSPSWPRARSTAA ATHCWWRTSWAAGRPRSTSLSWISQTPQLAHLV PLTFGAPH*PCPGMAPHMMGAVLYSPTASRSGT QPTRRGRN*PHAAAPLSTSRTCCLTTNISSVYVQ STCMEPVSQARSLNSQR*ERNLKSRRMKWRCQM MMRRSPRLITGQ*QSILNKKYLTSTTLRRD*DL GNLDRSFDL*KRKLEKSGQGSSSRHIQQKRKRIS GRRLAS*TASTTLSWSSVWMPLKKRPTSSWSWR SCQEGSCLSASLTRTLS*RSVSASSTCGRSRRE WSTSTSRASCTWTSSRRTSCVSTRRAPGSSSSTL VWPGGWRMRGL*RSSLAPQNLWLLK*STMSPSA TPQTCGASGSSATS*SVAFPPSWETTITKPWPT LPQPPGTSTTRHSMRSPTMPRISSAIC*RKI*KT AWTARSAFSIHG**KIPRTWRPRNSPRTG*RST WQEGNGRKRAML*EPLEDCPLWQ*SQGSVAGNP QQGHQPARSMQKN*NLKKMCPKLSLRLLLRKSLM *NPISLRPFAI*KLWREVLLDLTARLKDTQTPR LSGSKMTSQSGSPATSR*TTMRTGTAL*LLVMF AGMTMPSTPARLSTVLEKPPAQQSSLWKRWRKVK GKGKRKKSETKPEKSSF*VILKGLFL*NSKKKK KLKIVKAPSVIDYRLGHLWVDSSETAVDT*QRY *WALIYVSWHLKILVQLDFI*GNHQ*LD*PIDFR EKVTKPNIYLGKVINSPLECAHEK*GQNKSSGP QLSPEGSWGWEASLSPPPDSRELGFLPVLQQFI SESS*ATLFQSTLQMFKLSISLSPSTCQFC*LST CHECKH*GHYASSILKTGPCSWMTLASLGKYFS SKISRKSKLIPSLQMSSSFRHLVKNPWEKKNPC *CGFLCKPGFLFVLL*NISSERVVKIFVFEYRRN QFAEKLVLIIYWPR*NRFQLIKSWRTPCTLESP PR*PEFNTSSFSTLSKELDLPYGFHIFLLKCAS IMPCPQPLNIFLDLVNALRLASLGIFNFLSLHWH LNFFFIKLFEGHKQRP*LMIDLIHFLCVCV*HS KYFFFLFHCL*GATI*YF*GTF*EFLKNQFKIT SVQSYTVSTLEF*Y*SYVIFHSIFICFLLLVSNC QYFSFCF*NSYNIFHDSHSIATVYYNKGFLFDL AHSKLFSITFVFRI*PLCACMLCVCMCGVYVCY RLMPSWNCVNVLLVYYAIRMVNENTTTVVSSQFL NKGYHSACCLF

Frame 2

R*ASSEPALCACGRGAPPSAGAPAPGPPSPRGK DSSKLTETNSGQLKRKNKGGSRLPLLQFRATSG ASQPRASGRLSCPTGPVATTNNREDGDHGGCEA GCLVTHFQNLPQCGSLKS*LHAPDRGPCFHFAPS EPLHQRRSHRQVRRAGPGLPRAPGDMAQKRATH HQRGPLPAGLRHPGDFQPCDSCCP*GGQGKVYL *SHQWQWCSPGDSGVDSRRKFCEAAWSACCFQNL RG*IFSSSSGDPS*HLGGVPTKVCYQAGPSCGQ RRTDGTILLQDHWPAPTAGHLAQGKCSTAAECP CVCV*EERHAGSGNPWSQPR*RGSVHVPGGERVG EGLDVS*TFHPRFGQCQ*VICERNKSHQFRCQE RGDQCNLKGVEAGQSGGCSQKQELLQPPERWLP TLGCKQPASAPKGVQAGVMQGLAQNGPADPGPSE DFQLHHPAGRKSSAGTKSTRPGGPITFWRREEE ASSSPSSHLPHQAAWPGEPRCCEQGC*QENPHG GPEGFSIPQI*EQAPKPGGQGKSNCQVQM*SFRD SKA*SGLVPGRHPREETGRQH*GL*RCWLPLPL PAESPDQGQWDIQLHCFQRPRPAVL*LDPPSGK ACRDGGGPLLLQCPEGLRCY*GPGFCAAVLRTGD PSAPDHLAAEWAAHPVRSLHLRGRRG*APHPGC PAGGPWHLHLPS*ECLGAGVLQRLGHRP*KEE* QEE*VPSACGSQQAHCTHLPAGPL*SQSHGWKPG HYDCPSVRESTP*SHLAAQWE*DPRVRGLPL*T ERNSAQPLYPGSVPGGHGHVHLRGLEQRWRGPH PGRAHGTRASRWHPALVHQ*ASLSDSLPGPECPH LLRHSW*PLSYRALAQRWQSPLQRHWPLRGASE *GRVHPGSKEGAALACRPV*DPAQEPGWRMQLP GVTDATEQLCQSPSTGEGACQLRGPLWWRSWC*W WW**PLWVPEAWLASKRAGLARGGRRRGRARGA EEARGDEAAH*GGDPPAGGGAAGLPRPPGEEGE YKDPIGRRPEGDPSRADGFPCQPAAASEAKDCV* GREEGAQPPAGRFSLCPGQEGDFQDPRA*EGAT AKTCHPGFSLSAGWQEEITSREWQQQCRDPECQ GSGEFQAPEQCTAFRALETRGQRQAC*DPEANGQ RQACRDPEAHGQCQA**EPEIR*QRRTQERR*E *CELQERPCRDHR**KEIREPGDSPSLQAEAAR CSCGRGQEAAAPVPGVF*PPSHHHLDAERKDPQD HQVHHPLPGRLTLLRLHREGTA*GQRLIQVCSQ E*RWPGGVLLPSHRG*CSSQ*EHQGPRDEIPEA QELSSSRARN*E*CDCEKETCPQDTSEGSNAPSD HPVP*GPEGTRRRVSGAVWQSDRHSAHHLYLDE VPKADPGKRAHEGGEQREWQQAHHPGRAPGALR LLHTAGGEQAGQQAGPGQPHCRG*ARPPSWHTLC L*HSELLTDPVLVWLLI*WGQCCTVLQHRDLGL SQQDVEGTSHMPQHLFQRPGPAA*PRI*VPCTC NQRVWNQ*AKPGV*THNGRRET*RAEG*SGGVR* **EGARG*LPDSDNQY*TKSI*LLRH*GEIRIW EIWTGLSTCRKENSKSLGREVLQGIFSKREREY PAGD*HHELPPPP*AGPVCGCL*RKGQHRHGPGD RVRRGAV*AHH*RGL*ADGA*VHQVHAADLGGS GVHPQAGHRAPGPQAGEHHVCQQDGHQDQAHRL WSGQEAGECGVSEGPLWHPRICGS*SDQL*AHRL RHRHVEHRGHLLHPSQWPFPLHGRQR*RNLGQR YLSHLGLRRRGIR*DLRRCQGFHQQSAEERYEK PPGLHAVPSASMANERYQEHGGQETLQGPDEEVH GKKEMAENGQCCESHWKTVLYGNDLRAQWQEIL NRVTNQPAQCRKTRI*RRCVPSFP*GCC*GKAS CKTLFL*DHSRFRSCGGKCC*I*LQD*RIPRPRG CLVQR*PVNQGVPPLPDRLR*GRELLFNY**CL RG*RCQVHLQGCQQSWRSHLHSRAHCGNDGGR* RGRGRGRRVKQSQRKAVSKSY*KDYFSKTQKKKK NSR**KHLV**IIG*VICGLILQKQQLIPSSVI DGH*SMLVGTLRY*CS*ISFREITSNLTDQLIL ERK*PNQIFIWAKS*ILHLNALMKNKAKTRVLGH SSAQRVPGDGRPLSPHPLTLENWVFSQYSSNSF LKAVEPLYSKVHCRCSNSPFLFPLPPASFADSQ LVMSVSIKDIMLLRF*RQVPAHG*LWLP*ENIFL PKSVGNLNLSPLCRCLAASDIWLRTHGKKKILA NVVSFVNQDSYLCCYRISALNVW*RFLCLNIGE ISLLKS*S*LSIGHDETDFN**RAGELHVLWNLL QDSQSLIHLHSQHSPKNLTYLMGSIFFFLNVHQ SCLAPNL*IYS*TW*MHSDLRL*EFLTFFHYIG T*IFSL*SFLKVINKDHN***T*YISSVCVCNIP NTFFFFSTVCKVQQFNIFKGLFKSSLRTNLKLL QCNPTQYQH*NFDISLMLSSILFLSAFCC*FQT ASIFPFAFKIVTIFFMIATVLPQFIIIKGFYLI* RIQSFFLSLLCSEYNLCVRVCCVCACVAYMCVT G*CLLGIVLMFSWFIMPSEW*MRTLQL*SAHNF *IKDTTVHAVCS

Sequence NM_053029

GGCGCTGAGCGAGCTCGGAGCCCGCGCTGTGCGCCTGCGGCCGGGGCGCCCCGCCGAGCGCCGGTGCCCCGGCTCCCGGGCCGCCTTCGCCGCGCGGGAA GGATTCTTCAAAATTAACAGAAACCAATTCGGGCCAGCTGAAGAGAAAAAATAAAGGTGGCTCCCGGCTGCCTCTGCTGCAGTTCAGAGCAACTTCAGGA GCTTCCCAGCCGAGAGCTTCAGGACGCCTTTCCTGTCCCACTGGCCCAGTTGCCACAACAAACAACAGAGAAGACGGTGACCATGGGGGATGTGAAGCTG GTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTGGATCCCTCAAGAGTTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCTC GGAACCTCTGCATCAAAGAAGGAGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTACCCAGAGCCCCAGGTGACATGGCACAGAAACGGGCAACCCAT CACCAGCGGGGGCCGCTTCCTGCTGGATTGCGGCATCCGGGGGACTTTCAGCCTTGTGATTCATGCTGTCCATGAGGAGGACAGGGGAAAGTATACCTGT GAAGCCACCAATGGCAGTGGTGCTCGCCAGGTGACAGTGGAGTTGACAGTAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACCT TAGGGGATAGATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGCCCACCAAAGTTTGCTACCAAGCTGGGCCGAGTTGTGGTCAA AGAAGGACAGATGGGACGATTCTCCTGCAAGATCACTGGCCGGCCCCAACCGCAGGTCACCTGGCTCAAGGGAAATGTTCCACTGCAGCCGAGTGCCCGT GTGTCTGTGTCTGAGAAGAACGGCATGCAGGTTCTGGAAATCCATGGAGTCAACCAAGATGACGTGGGAGTGTACACGTGCCTGGTGGTGAACGGGTCGG GGAAGGCCTCGATGTCAGCTGAACTTTCCATCCAAGGTTTGGACAGTGCCAATAGGTCATTTGTGAGAGAAACAAAAGCCACCAATTCAGATGTCAGGAA AGAGGTGACCAATGTAATCTCAAAGGAGTCGAAGCTGGACAGTCTGGAGGCTGCAGCCAAAAGCAAGAACTGCTCCAGCCCCCAGAGAGGTGGCTCCCCA CCCTGGGCTGCAAACAGCCAGCCTCAGCCCCCAAGGGAGTCCAAGCTGGAGTCATGCAAGGACTCGCCCAGAACGGCCCCGCAGACCCCGGTCCTTCAGA AGACTTCCAGCTCCATCACCCTGCAGGCCGCAAGAGTTCAGCCGGAACCAAGAGCACCAGGCCTGGGGGTCCTATCACCTTCTGGAGAAGAGAGGAAGAG GCCAGCTCCTCCCCGTCCAGCCACCTTCCCCACCAGGCAGCCTGGCCTGGGGAGCCAAGATGTTGTGAGCAAGGCTGCTAACAGGAGAATCCCCATGGAG GGCCAGAGGGATTCAGCATTCCCCAAATTTGAGAGCAAGCCCCAAAGCCAGGAGGTCAAGGAAAATCAAACTGTCAAGTTCAGATGTGAAGTTTCCGGGA TTCCAAAGCCTGAAGTGGCCTGGTTCCTGGAAGGCACCCCCGTGAGGAGACAGGAAGGCAGCATTGAGGTTTATGAAGATGCTGGCTCCCATTACCTCTG CCTGCTGAAAGCCCGGACCAGGGACAGTGGGACATACAGCTGCACTGCTTCCAACGCCCAAGGCCAGCTGTCCTGTAGCTGGACCCTCCAAGTGGAAAGG CTTGCCGTGATGGAGGTGGCCCCCTCCTTCTCCAGTGTCCTGAAGGACTGCGCTGTTATTGAGGGCCAGGATTTTGTGCTGCAGTGCTCCGTACGGGGGA CCCCAGTGCCCCGGATCACTTGGCTGCTGAATGGGCAGCCCATCCAGTACGCTCGCTCCACCTGCGAGGCCGGCGTGGCTGAGCTCCACATCCAGGATGC CCTGCCGGAGGACCATGGCACCTACACCTGCCTAGCTGAGAATGCCTTGGGGCAGGTGTCCTGCAGCGCCTGGGTCACCGTCCATGAAAAGAAGAGTAGC AGGAAGAGTGAGTACCTTCTGCCTGTGGCTCCCAGCAAGCCCACTGCACCCATCTTCCTGCAGGGCCTCTCTGATCTCAAAGTCATGGATGGAAGCCAGG TCACTATGACTGTCCAAGTGTCAGGGAATCCACCCCCTGAAGTCATCTGGCTGCACAATGGGAATGAGATCCAAGAGTCAGAGGACTTCCACTTTGAACA GAGAGGAACTCAGCACAGCCTTTGTATCCAGGAAGTGTTCCCGGAGGACACGGGCACGTACACCTGCGAGGCCTGGAACAGCGCTGGAGAGGTCCGCACC CAGGCCGTGCTCACGGTACAAGAGCCTCACGATGGCACCCAGCCCTGGTTCATCAGTAAGCCTCGCTCAGTGACAGCCTCCCTGGGCCAGAGTGTCCTCA TCTCCTGCGCCATAGCTGGTGACCCCTTTCCTACCGTGCACTGGCTCAGAGATGGCAAAGCCCTCTGCAAAGACACTGGCCACTTCGAGGTGCTTCAGAA TGAGGACGTGTTCACCCTGGTTCTAAAGAAGGTGCAGCCCTGGCATGCCGGCCAGTATGAGATCCTGCTCAAGAACCGGGTTGGCGAATGCAGTTGCCAG GTGTCACTGATGCTACAGAACAGCTCTGCCAGAGCCCTTCCACGGGGGAGGGAGCCTGCCAGCTGCGAGGACCTCTGTGGTGGAGGAGTTGGTGCTGATG GTGGTGGTAGTGACCGCTATGGGTCCCTGAGGCCTGGCTGGCCAGCAAGAGGGCAGGGTTGGCTAGAGGAGGAAGACGGCGAGGACGTGCGAGGGGTGCT GAAGAGGCGCGTGGAGACGAGGCAGCACACTGAGGAGGCGATCCGCCAGCAGGAGGTGGAGCAGCTGGACTTCCGAGACCTCCTGGGGAAGAAGGTGAGT ACAAAGACCCTATCGGAAGACGACCTGAAGGAGATCCCAGCCGAGCAGATGGATTTCCGTGCCAACCTGCAGCGGCAAGTGAAGCCAAAGACTGTGTCTG AGGAAGAGAGGAAGGTGCACAGCCCCCAGCAGGTCGATTTTCGCTCTGTCCTGGCCAAGAAGGGGACTTCCAAGACCCCCGTGCCTGAGAAGGTGCCACC GCCAAAACCTGCCACCCCGGATTTTCGCTCAGTGCTGGGTGGCAAGAAGAAATTACCAGCAGAGAATGGCAGCAGCAGTGCCGAGACCCTGAATGCCAAG GCAGTGGAGAGTTCCAAGCCCCTGAGCAATGCACAGCCTTCAGGGCCCTTGAAACCCGTGGGCAACGCCAAGCCTGCTGAGACCCTGAAGCCAATGGGCA ACGCCAAGCCTGCCGAGACCCTGAAGCCCATGGGCAATGCCAAGCCTGATGAGAACCTGAAATCCGCTAGCAAAGAAGAACTCAAGAAAGACGTTAAGAA TGATGTGAACTGCAAGAGAGGCCATGCAGGGACCACAGATAATGAAAAGAGATCAGAGAGCCAGGGGACAGCCCCAGCCTTCAAGCAGAAGCTGCAAGAT GTTCATGTGGCAGAGGGCAAGAAGCTGCTGCTCCAGTGCCAGGTGTCTTCTGACCCCCCAGCCACCATCATCTGGACGCTGAACGGAAAGACCCTCAAGA CCACCAAGTTCATCATCCTCTCCCAGGAAGGCTCACTCTGCTCCGTCTCCATCGAGAAGGCACTGCCTGAGGACAGAGGCTTATACAAGTGTGTAGCCAA GAATGACGCTGGCCAGGCGGAGTGCTCCTGCCAAGTCACCGTGGATGATGCTCCAGCCAGTGAGAACACCAAGGCCCCAGAGATGAAATCCCGGAGGCCC AAGAGCTCTCTTCCTCCCGTGCTAGGAACTGAGAGTGATGCGACTGTGAAAAAGAAACCTGCCCCCAAGACACCTCCGAAGGCAGCAATGCCCCCTCAGA TCATCCAGTTCCCTGAGGACCAGAAGGTACGCGCAGGAGAGTCAGTGGAGCTGTTTGGCAAAGTGACAGGCACTCAGCCCATCACCTGTACCTGGATGAA GTTCCGAAAGCAGATCCAGGAAAGCGAGCACATGAAGGTGGAGAACAGCGAGAATGGCAGCAAGCTCACCATCCTGGCCGCGCGCCAGGAGCACTGCGGC TGCTACACACTGCTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAGGTCAACCTCACTGTCGTGGATAAGCCAGACCCCCCAGCTGGCACACCTTGTG CCTCTGACATTCGGAGCTCCTCACTGACCCTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAGCATCGAGATCTGGGACTC AGCCAACAAGACGTGGAAGGAACTAGCCACATGCCGCAGCACCTCTTTCAACGTCCAGGACCTGCTGCCTGACCACGAATATAAGTTCCGTGTACGTGCA ATCAACGTGTATGGAACCAGTGAGCCAAGCCAGGAGTCTGAACTCACAACGGTAGGAGAGAAACCTGAAGAGCCGAAGGATGAAGTGGAGGTGTCAGATG ATGATGAGAAGGAGCCCGAGGTTGATTACCGGACAGTGACAATCAATACTGAACAAAAAGTATCTGACTTCTACGACATTGAGGAGAGATTAGGATCCTG TTTGAGCGCATCATTGACGAGGACTTTGAGCTGACGGAGCGTGAGTGCATCAAGTACATGCGGCAGATCTCGGAGGGAGTGGAGTACATCCACAAGCAGG GCATCGTGCACCTGGACCTCAAGCCGGAGAACATCATGTGTGTCAACAAGACGGGCACCAGGATCAAGCTCATCGACTTTGGTCTGGCCAGGAGGCTGGA GAATGCGGGGTCTCTGAAGGTCCTCTTTGGCACCCCAGAATTTGTGGCTCCTGAAGTGATCAACTATGAGCCCATCGGCTACGCCACAGACATGTGGAGC ATCGGGGTCATCTGCTACATCCTAGTCAGTGGCCTTTCCCCCTTCATGGGAGACAACGATAACGAAACCTTGGCCAACGTTACCTCAGCCACCTGGGACT TCGACGACGAGGCATTCGATGAGATCTCCGACGATGCCAAGGATTTCATCAGCAATCTGCTGAAGAAAGATATGAAAAACCGCCTGGACTGCACGCAGTG CCTTCAGCATCCATGGCTAATGAAAGATACCAAGAACATGGAGGCCAAGAAACTCTCCAAGGACCGGATGAAGAAGTACATGGCAAGAAGGAAATGGCAG AAAACGGGCAATGCTGTGAGAGCCATTGGAAGACTGTCCTCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCTCAACAGGGTCACCAACCAGCC CGCTCAATGCAGAAAAACTAGAATCTGAAGAAGATGTGTCCCAAGCTTTCCTTGAGGCTGTTGCTGAGGAAAAGCCTCATGTAAAACCCTATTTCTCTAA GACCATTCGCGATTTAGAAGTTGTGGAGGGAAGTGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCCGAGGTTGTCTGGTTCAAAGATGAC CAGTCAATCAGGGAGTCCCGCCACTTCCAGATAGACTACGATGAGGACGGGAACTGCTCTTTAATTATTAGTGATGTTTGCGGGGATGACGATGCCAAGT ACACCTGCAAGGCTGTCAACAGTCTTGGAGAAGCCACCTGCACAGCAGAGCTCATTGTGGAAACGATGGAGGAAGGTGAAGGGGAAGGGGAAGAGGAAGA AGAGTGAAACAAAGCCAGAGAAAAGCAGTTTCTAAGTCATATTAAAAGGACTATTTCTCTAAAACTCAAAAAAAAAAAAAAAACTCAAGATAGTAAAAGC ACCTAGTGTGATAGATTATCGGTTAGGTCATTTGTGGGTTGATTCTTCAGAAACAGCAGTTGATACCTAGCAGCGTTATTGATGGGCATTAATCTATGTT AGTTGGCACCTTAAGATACTAGTGCAGCTAGATTTCATTTAGGGAAATCACCAGTAACTTGACTGACCAATTGATTTTAGAGAGAAAGTAACCAAACCAA ATATTTATCTGGGCAAAGTCATAAATTCTCCACTTGAATGCGCTCATGAAAAATAAGGCCAAAACAAGAGTTCTGGGCCACAGCTCAGCCCAGAGGGTTC CTGGGGATGGGAGGCCTCTCTCTCCCCACCCCCTGACTCTAGAGAACTGGGTTTTCTCCCAGTACTCCAGCAATTCATTTCTGAAAGCAGTTGAGCCACT TTATTCCAAAGTACACTGCAGATGTTCAAACTCTCCATTTCTCTTTCCCCTTCCACCTGCCAGTTTTGCTGACTCTCAACTTGTCATGAGTGTAAGCATT AAGGACATTATGCTTCTTCGATTCTGAAGACAGGTCCCTGCTCATGGATGACTCTGGCTTCCTTAGGAAAATATTTTTCTTCCAAAATCAGTAGGAAATC TAAACTTATCCCCTCTTTGCAGATGTCTAGCAGCTTCAGACATTTGGTTAAGAACCCATGGGAAAAAAAAAATCCTTGCTAATGTGGTTTCCTTTGTAAA CCAGGATTCTTATTTGTGCTGTTATAGAATATCAGCTCTGAACGTGTGGTAAAGATTTTTGTGTTTGAATATAGGAGAAATCAGTTTGCTGAAAAGTTAG TCTTAATTATCTATTGGCCACGATGAAACAGATTTCAACTGATAAAGAGCTGGAGAACTCCATGTACTTTGGAATCTCCTCCAAGATAGCCAGAGTTTAA TACATCTTCATTCTCAACACTCTCCAAAGAACTTGACCTACCTTATGGGTTCCATATTTTTCTTCTTAAATGTGCATCAATCATGCCTTGCCCCCAACCT TTAAATATATTCTTAGACCTGGTAAATGCACTCAGACTTGCGTCTTTAGGAATTTTTAACTTTCTTTCACTACATTGGCACTTAAATTTTTTCTTTATAA AGCTTTTTGAAGGTCATAAACAAAGACCATAATTGATGATAGACCTAATACATTTCCTCTGTGTGTGTGTGTAACATTCCAAATACTTTTTTTTTCTTTT CCACTGTTTGTAAGGTGCAACAATTTAATATTTTTAAGGGACTTTTTAAGAGTTCCTTAAGAACCAATTTAAAATTACTTCAGTGCAATCCTACACAGTA TCAACATTAGAATTTTGATATTAGTCTTATGTTATCTTCCATTCTATTTTTATCTGCTTTTTGCTGCTAGTTTCAAACTGCCAGTATTTTTCCTTTTGCT TTTAAAATAGTTACAATATTTTTCATGATAGCCACAGTATTGCCACAGTTTATTATAATAAAGGGTTTTTATTTGATTTAGCGCATTCAAAGCTTTTTTC TATCACTTTTGTGTTCAGAATATAACCTTTGTGTGCGTGTATGTTGTGTGTGTGCATGTGTGGCGTATATGTGTGTTACAGGTTAATGCCTTCTTGGAAT TGTGTTAATGTTCTCTTGGTTTATTATGCCATCAGAATGGTAAATGAGAACACTACAACTGTAGTCAGCTCACAATTTTTAAATAAAGGATACCACAGTG CATGCTGTTTGTTCAA

refseq_MYLK.F4 (forward primer)
refseq_MYLK.R4 (reverse primer)
Single exon skipping

Frame 0

GAERARSPRCAPAAGAPRRAPVPRLPGRLRRAG RILQN*QKPIRAS*REKIKVAPGCLCCSSEQLQE LPSRELQDAFPVPLAQLPQQTTEKTVTMGDVKL VASSHISKTSLSVDPSRVDSMPLTEAPAFILPP RNLCIKEGATAKFEGRVRGYPEPQVTWHRNGQPI TSGGRFLLDCGIRGTFSLVIHAVHEEDRGKYTC EATNGSGARQVTVELTVEGSFAKQLGQPVVSKT LGDRFSAPAVETRPSIWGECPPKFATKLGRVVVK EGQMGRFSCKITGRPQPQVTWLKGNVPLQPSAR VSVSEKNGMQVLEIHGVNQDDVGVYTCLVVNGS GKASMSAELSIQGLDSANRSFVRETKATNSDVRK EVTNVISKESKLDSLEAAAKSKNCSSPQRGGSP PWAANSQPQPPRESKLESCKDSPRTAPQTPVLQ KTSSSITLQAARVQPEPRAPGLGVLSPSGEERKR PAPPRPATFPTRQPGLGSQDVVSKAANRRIPME GQRDSAFPKFESKPQSQEVKENQTVKFRCEVSG IPKPEVAWFLEGTPVRRQEGSIEVYEDAGSHYLC LLKARTRDSGTYSCTASNAQGQLSCSWTLQVER LAVMEVAPSFSSVLKDCAVIEGQDFVLQCSVRG TPVPRITWLLNGQPIQYARSTCEAGVAELHIQDA LPEDHGTYTCLAENALGQVSCSAWVTVHEKKSS RKSEYLLPVAPSKPTAPIFLQGLSDLKVMDGSQ VTMTVQVSGNPPPEVIWLHNGNEIQESEDFHFEQ RGTQHSLCIQEVFPEDTGTYTCEAWNSAGEVRT QAVLTVQEPHDGTQPWFISKPRSVTASLGQSVL ISCAIAGDPFPTVHWLRDGKALCKDTGHFEVLQN EDVFTLVLKKVQPWHAGQYEILLKNRVGECSCQ VSLMLQNSSARALPRGREPASCEDLCGGGVGAD GGGSDRYGSLRPGWPARGQGWLEEEDGEDVRGVL KRRVETRQHTEEAIRQQEVEQLDFRDLLGKKVS TKTLSEDDLKEIPAEQMDFRANLQRQVKPKTVS EEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVPP PKPATPDFRSVLGGKKKLPAENGSSSAETLNAK AVESSKPLSNAQPSGPLKPVGNAKPAETLKPMG NAKPAETLKPMGNAKPDENLKSASKEELKKDVKN DVNCKRGHAGTTDNEKRSESQGTAPAFKQKLQD VHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLK TTKFIILSQEGSLCSVSIEKALPEDRGLYKCVAK NDAGQAECSCQVTVDDAPASENTKAPEMKSRRP KSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQ IIQFPEDQKVRAGESVELFGKVTGTQPITCTWMK FRKQIQESEHMKVENSENGSKLTILAARQEHCG CYTLLVENKLGSRQAQVNLTVVDKPDPPAGTPC ASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDS ANKTWKELATCRSTSFNVQDLLPDHEYKFRVRA INVYGTSEPSQESELTTVGEKPEEPKDEVEVSD DDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSC LSASLTRTLS*RSVSASSTCGRSRREWSTSTSR ASCTWTSSRRTSCVSTRRAPGSSSSTLVWPGGW RMRGL*RSSLAPQNLWLLK*STMSPSATPQTCGA SGSSATS*SVAFPPSWETTITKPWPTLPQPPGT STTRHSMRSPTMPRISSAIC*RKI*KTAWTARS AFSIHG**KIPRTWRPRNSPRTG*RSTWQEGNGR KRAML*EPLEDCPLWQ*SQGSVAGNPQQGHQPA RSMQKN*NLKKMCPKLSLRLLLRKSLM*NPISL RPFAI*KLWREVLLDLTARLKDTQTPRLSGSKMT SQSGSPATSR*TTMRTGTAL*LLVMFAGMTMPS TPARLSTVLEKPPAQQSSLWKRWRKVKGKGKRK KSETKPEKSSF*VILKGLFL*NSKKKKKLKIVKA PSVIDYRLGHLWVDSSETAVDT*QRY*WALIYV SWHLKILVQLDFI*GNHQ*LD*PIDFREKVTKP NIYLGKVINSPLECAHEK*GQNKSSGPQLSPEGS WGWEASLSPPPDSRELGFLPVLQQFISESS*AT LFQSTLQMFKLSISLSPSTCQFC*LSTCHECKH *GHYASSILKTGPCSWMTLASLGKYFSSKISRKS KLIPSLQMSSSFRHLVKNPWEKKNPC*CGFLCK PGFLFVLL*NISSERVVKIFVFEYRRNQFAEKL VLIIYWPR*NRFQLIKSWRTPCTLESPPR*PEFN TSSFSTLSKELDLPYGFHIFLLKCASIMPCPQP LNIFLDLVNALRLASLGIFNFLSLHWHLNFFFI KLFEGHKQRP*LMIDLIHFLCVCV*HSKYFFFLF HCL*GATI*YF*GTF*EFLKNQFKITSVQSYTV STLEF*Y*SYVIFHSIFICFLLLVSNCQYFSFC F*NSYNIFHDSHSIATVYYNKGFLFDLAHSKLFS ITFVFRI*PLCACMLCVCMCGVYVCYRLMPSWN CVNVLLVYYAIRMVNENTTTVVSSQFLNKGYHS ACCLF

Frame 1

ALSELGARAVRLRPGRPAERRCPGSRAAFAARE GFFKINRNQFGPAEEKK*RWLPAASAAVQSNFR SFPAESFRTPFLSHWPSCHNKQQRRR*PWGM*SW LPRHTFPKPPSVWIPQELTPCP*QRPLLSFCPL GTSASKKEPPPSSKGGSGVTQSPR*HGTETGNP SPAGAASCWIAASGGLSAL*FMLSMRRTGESIPV KPPMAVVLAR*QWS*Q*KEVLRSSLVSLLFPKP *GIDFQLQQWRPVLASGGSAHQSLLPSWAELWS KKDRWDDSPARSLAGPNRRSPGSREMFHCSRVPV CLCLRRTACRFWKSMESTKMTWECTRAWW*TGR GRPRCQLNFPSKVWTVPIGHL*EKQKPPIQMSG KR*PM*SQRSRSWTVWRLQPKARTAPAPREVAPH PGLQTASLSPQGSPSWSHARTRPERPRRPRSFR RLPAPSPCRPQEFSRNQEHQAWGSYHLLEKRGR GQLLPVQPPSPPGSLAWGAKML*ARLLTGESPWR ARGIQHSPNLRASPKARRSRKIKLSSSDVKFPG FQSLKWPGSWKAPP*GDRKAALRFMKMLAPITS AC*KPGPGTVGHTAALLPTPKASCPVAGPSKWKG LP*WRWPPPSPVS*RTALLLRARILCCSAPYGG PQCPGSLGC*MGSPSSTLAPPARPAWLSSTSRM PCRRTMAPTPA*LRMPWGRCPAAPGSPSMKRRVA GRVSTFCLWLPASPLHPSSCRASLISKSWMEAR SL*LSKCQGIHPLKSSGCTMGMRSKSQRTSTLN REELSTAFVSRKCSRRTRARTPARPGTALERSAP RPCSRYKSLTMAPSPGSSVSLAQ*QPPWARVSS SPAP*LVTPFLPCTGSEMAKPSAKTLATSRCFR MRTCSPWF*RRCSPGMPASMRSCSRTGLANAVAR CH*CYRTALPEPFHGGGSLPAARTSVVEELVLM VVVVTAMGP*GLAGQQEGRVG*RRKTARTCEGC *RGAWRRGSTLRRRSASRRWSSWTSETSWGRR*V QRPYRKTT*RRSQPSRWISVPTCSGK*SQRLCL RKRGRCTAPSRSIFALSWPRRGLPRPPCLRRCH RQNLPPRIFAQCWVARRNYQQRMAAAVPRP*MPR QWRVPSP*AMHSLQGP*NPWATPSLLRP*SQWA TPSLPRP*SPWAMPSLMRT*NPLAKKNSRKTLR MM*TAREAMQGPQIMKRDQRARGQPQPSSRSCKM FMWQRARSCCSSARCLLTPQPPSSGR*TERPSR PPSSSSSPRKAHSAPSPSRRHCLRTEAYTSV*P RMTLARRSAPAKSPWMMLQPVRTPRPQR*NPGGP RALFLPC*ELRVMRL*KRNLPPRHLRRQQCPLR SSSSLRTRRYAQESQWSCLAK*QALSPSPVPG* SSESRSRKAST*RWRTARMAASSPSWPRARSTAA ATHCWWRTSWAAGRPRSTSLSWISQTPQLAHLV PLTFGAPH*PCPGMAPHMMGAVLYSPTASRSGT QPTRRGRN*PHAAAPLSTSRTCCLTTNISSVYVQ STCMEPVSQARSLNSQR*ERNLKSRRMKWRCQM MMRRSPRLITGQ*QSILNKKYLTSTTLRRD*DP V*AHH*RGL*ADGA*VHQVHAADLGGSGVHPQAG HRAPGPQAGEHHVCQQDGHQDQAHRLWSGQEAG ECGVSEGPLWHPRICGS*SDQL*AHRLRHRHVE HRGHLLHPSQWPFPLHGRQR*RNLGQRYLSHLGL RRRGIR*DLRRCQGFHQQSAEERYEKPPGLHAV PSASMANERYQEHGGQETLQGPDEEVHGKKEMA ENGQCCESHWKTVLYGNDLRAQWQEILNRVTNQP AQCRKTRI*RRCVPSFP*GCC*GKASCKTLFL* DHSRFRSCGGKCC*I*LQD*RIPRPRGCLVQR* PVNQGVPPLPDRLR*GRELLFNY**CLRG*RCQV HLQGCQQSWRSHLHSRAHCGNDGGR*RGRGRGR RVKQSQRKAVSKSY*KDYFSKTQKKKKNSR**K HLV**IIG*VICGLILQKQQLIPSSVIDGH*SML VGTLRY*CS*ISFREITSNLTDQLILERK*PNQ IFIWAKS*ILHLNALMKNKAKTRVLGHSSAQRV PGDGRPLSPHPLTLENWVFSQYSSNSFLKAVEPL YSKVHCRCSNSPFLFPLPPASFADSQLVMSVSI KDIMLLRF*RQVPAHG*LWLP*ENIFLPKSVGN LNLSPLCRCLAASDIWLRTHGKKKILANVVSFVN QDSYLCCYRISALNVW*RFLCLNIGEISLLKS* S*LSIGHDETDFN**RAGELHVLWNLLQDSQSL IHLHSQHSPKNLTYLMGSIFFFLNVHQSCLAPNL *IYS*TW*MHSDLRL*EFLTFFHYIGT*IFSL* SFLKVINKDHN***T*YISSVCVCNIPNTFFFF STVCKVQQFNIFKGLFKSSLRTNLKLLQCNPTQY QH*NFDISLMLSSILFLSAFCC*FQTASIFPFA FKIVTIFFMIATVLPQFIIIKGFYLI*RIQSFF LSLLCSEYNLCVRVCCVCACVAYMCVTG*CLLGI VLMFSWFIMPSEW*MRTLQL*SAHNF*IKDTTV HAVCS

Frame 2

R*ASSEPALCACGRGAPPSAGAPAPGPPSPRGK DSSKLTETNSGQLKRKNKGGSRLPLLQFRATSG ASQPRASGRLSCPTGPVATTNNREDGDHGGCEA GCLVTHFQNLPQCGSLKS*LHAPDRGPCFHFAPS EPLHQRRSHRQVRRAGPGLPRAPGDMAQKRATH HQRGPLPAGLRHPGDFQPCDSCCP*GGQGKVYL *SHQWQWCSPGDSGVDSRRKFCEAAWSACCFQNL RG*IFSSSSGDPS*HLGGVPTKVCYQAGPSCGQ RRTDGTILLQDHWPAPTAGHLAQGKCSTAAECP CVCV*EERHAGSGNPWSQPR*RGSVHVPGGERVG EGLDVS*TFHPRFGQCQ*VICERNKSHQFRCQE RGDQCNLKGVEAGQSGGCSQKQELLQPPERWLP TLGCKQPASAPKGVQAGVMQGLAQNGPADPGPSE DFQLHHPAGRKSSAGTKSTRPGGPITFWRREEE ASSSPSSHLPHQAAWPGEPRCCEQGC*QENPHG GPEGFSIPQI*EQAPKPGGQGKSNCQVQM*SFRD SKA*SGLVPGRHPREETGRQH*GL*RCWLPLPL PAESPDQGQWDIQLHCFQRPRPAVL*LDPPSGK ACRDGGGPLLLQCPEGLRCY*GPGFCAAVLRTGD PSAPDHLAAEWAAHPVRSLHLRGRRG*APHPGC PAGGPWHLHLPS*ECLGAGVLQRLGHRP*KEE* QEE*VPSACGSQQAHCTHLPAGPL*SQSHGWKPG HYDCPSVRESTP*SHLAAQWE*DPRVRGLPL*T ERNSAQPLYPGSVPGGHGHVHLRGLEQRWRGPH PGRAHGTRASRWHPALVHQ*ASLSDSLPGPECPH LLRHSW*PLSYRALAQRWQSPLQRHWPLRGASE *GRVHPGSKEGAALACRPV*DPAQEPGWRMQLP GVTDATEQLCQSPSTGEGACQLRGPLWWRSWC*W WW**PLWVPEAWLASKRAGLARGGRRRGRARGA EEARGDEAAH*GGDPPAGGGAAGLPRPPGEEGE YKDPIGRRPEGDPSRADGFPCQPAAASEAKDCV* GREEGAQPPAGRFSLCPGQEGDFQDPRA*EGAT AKTCHPGFSLSAGWQEEITSREWQQQCRDPECQ GSGEFQAPEQCTAFRALETRGQRQAC*DPEANGQ RQACRDPEAHGQCQA**EPEIR*QRRTQERR*E *CELQERPCRDHR**KEIREPGDSPSLQAEAAR CSCGRGQEAAAPVPGVF*PPSHHHLDAERKDPQD HQVHHPLPGRLTLLRLHREGTA*GQRLIQVCSQ E*RWPGGVLLPSHRG*CSSQ*EHQGPRDEIPEA QELSSSRARN*E*CDCEKETCPQDTSEGSNAPSD HPVP*GPEGTRRRVSGAVWQSDRHSAHHLYLDE VPKADPGKRAHEGGEQREWQQAHHPGRAPGALR LLHTAGGEQAGQQAGPGQPHCRG*ARPPSWHTLC L*HSELLTDPVLVWLLI*WGQCCTVLQHRDLGL SQQDVEGTSHMPQHLFQRPGPAA*PRI*VPCTC NQRVWNQ*AKPGV*THNGRRET*RAEG*SGGVR* **EGARG*LPDSDNQY*TKSI*LLRH*GEIRIL FERIIDEDFELTERECIKYMRQISEGVEYIHKQ GIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLE NAGSLKVLFGTPEFVAPEVINYEPIGYATDMWS IGVICYILVSGLSPFMGDNDNETLANVTSATWD FDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQC LQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQ KTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTS PLNAEKLESEEDVSQAFLEAVAEEKPHVKPYFSK TIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDD QSIRESRHFQIDYDEDGNCSLIISDVCGDDDAK YTCKAVNSLGEATCTAELIVETMEEGEGEGEEEE E*NKAREKQFLSHIKRTISLKLKKKKKTQDSKS T*CDRLSVRSFVG*FFRNSS*YLAALLMGINLC *LAP*DTSAARFHLGKSPVT*LTN*F*RESNQTK YLSGQSHKFST*MRS*KIRPKQEFWATAQPRGF LGMGGLSLPTP*L*RTGFSPSTPAIHF*KQLSH FIPKYTADVQTLHFSFPFHLPVLLTLNLS*V*AL RTLCFFDSEDRSLLMDDSGFLRKIFFFQNQ*EI *TYPLFADV*QLQTFG*EPMGKKKSLLMWFPL* TRILICAVIEYQL*TCGKDFCV*I*EKSVC*KVS LNYLLATMKQISTDKELENSMYFGISSKIARV* YIFILNTLQRT*PTLWVPYFSS*MCINHALPPT FKYILRPGKCTQTCVFRNF*LSFTTLALKFFLYK AF*RS*TKTIIDDRPNTFPLCVCVTFQILFFSF PLFVRCNNLIFLRDFLRVP*EPI*NYFSAILHS INIRILILVLCYLPFYFYLLFAASFKLPVFFLLL LK*LQYFS**PQYCHSLL**RVFI*FSAFKAFF YHFCVQNITFVCVYVVCVHVWRICVLQVNAFLE LC*CSLGLLCHQNGK*EHYNCSQLTIFK*RIPQC MLFVQ