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Sequences

Sequence NM_001029854

GCGGCCGCGGGCGCGGGCGGGCGCGCGGGGGAGCCCGGCCGAGGGATGGGCTGCGCCCCCAGCATCCATGTCTCGCAGAGCGGCGTGATCTACTGCCGGG
ACTCGGACGAGTCCAGCTCGCCCCGCCAGACCACCAGCGTGTCGCAGGGCCCGGCGGCACCCCTGCCCGGCCTCTTCGTCCAGACCGACGCCGCCGACGC
CATCCCCCCGAGCCGCGCGTCGGGACCCCCCAGCGTAGCCCGCGTCCGCAGGGCCCGCACCGAGCTGGGCAGCGGTAGCAGCGCGGGTTCCGCAGCCCCC
GCCGCGACCACCAGCAGGGGCCGGAGGCGCCACTGCTGCAGCAGCGCCGAGGCCGAGACTCAGACCTGCTACACCAGCGTGAAGCAGGTGTCTTCTGCGG
AGGTGCGCATCGGGCCCATGAGACTGACGCAGGACCCTATTCAGGTTTTGCTGATCTTTGCAAAGGAAGATAGTCAGAGCGATGGCTTCTGGTGGGCCTG
CGACAGAGCTGGTTATAGATGCAATATTGCTCGGACTCCAGAGTCAGCCCTTGAATGCTTTCTTGATAAGCATCATGAAATTATTGTAATTGATCATAGA
CAAACTCAGAACTTCGATGCAGAAGCAGTGTGCAGGTCGATCCGGGCCACAAATCCCTCCGAGCACACGGTGATCCTCGCAGTGGTTTCGCGAGTATCGG
ATGACCATGAAGAGGCGTCAGTCCTTCCTCTTCTCCACGCAGGCTTCAACAGGAGATTTATGGAGAATAGCAGCATAATTGCTTGCTATAATGAACTGAT
TCAAATAGAACATGGGGAAGTTCGCTCCCAGTTCAAATTACGGGCCTGTAATTCAGTGTTTACAGCATTAGATCACTGTCATGAAGCCATAGAAATAACA
AGCGATGACCACGTGATTCAGGAGTGGCAGGGGGTTTACTATGCCAGACGGAAATCCGGGGACAGCATCCAACAGCACGTGAAGATCACCCCAGTGATTG
GCCAAGGAGGGAAAATTAGGCATTTTGTCTCGCTCAAGAAACTGTGTTGTACCACTGACAATAATAAGCAGATTCACAAGATTCATCGTGATTCAGGAGA
CAATTCTCAGACAGAGCCTCATTCATTCAGATATAAGAACAGGAGGAAAGAGTCCATTGACGTGAAATCGATATCATCTCGAGGCAGTGATGCACCAAGC
CTGCAGAATCGTCGCTATCCGTCCATGGCGAGGATCCACTCCATGACCATCGAGGCTCCCATCACAAAGGTTATAAATATAATCAATGCAGCCCAAGAAA
ACAGCCCAGTCACAGTAGCGGAAGCCTTGGACAGAGTTCTAGAGATTTTACGGACCACAGAACTGTACTCCCCTCAGCTGGGTACCAAAGATGAAGATCC
TCACACCAGTGATCTTGTTGGAGGCCTGATGACTGACGGCTTGAGAAGACTGTCAGGAAACGAGTATGTGTTTACTAAGAATGTGCACCAGAGTCACAGT
CACCTTGCAATGCCAATAACCATCAATGATGTTCCCCCTTGTATCTCTCAATTACTTGATAATGAGGAGAGTTGGGACTTCAACATCTTTGAATTGGAAG
CCATTACGCATAAAAGGCCATTGGTTTATCTGGGCTTAAAGGTCTTCTCTCGGTTTGGAGTATGTGAATTTTTAAACTGTTCTGAAACCACTCTTCGGGC
CTGGTTCCAAGTGATCGAAGCCAACTACCACTCTTCCAATGCCTACCACAACTCCACCCATGCTGCCGACGTCCTGCACGCCACCGCTTTCTTTCTTGGA
AAGGAAAGAGTAAAGGGAAGCCTCGATCAGTTGGATGAGGTGGCAGCCCTCATTGCTGCCACAGTCCATGACGTGGATCACCCGGGAAGGACCAACTCTT
TCCTCTGCAATGCAGGCAGTGAGCTTGCTGTGCTCTACAATGACACTGCTGTTCTGGAGAGTCACCACACCGCCCTGGCCTTCCAGCTCACGGTCAAGGA
CACCAAATGCAACATTTTCAAGAATATTGACAGGAACCATTATCGAACGCTGCGCCAGGCTATTATTGACATGGTTTTGGCAACAGAGATGACAAAACAC
TTTGAACATGTGAATAAGTTTGTGAACAGCATCAACAAGCCAATGGCAGCTGAGATTGAAGGCAGCGACTGTGAATGCAACCCTGCTGGGAAGAACTTCC
CTGAAAACCAAATCCTGATCAAACGCATGATGATTAAGTGTGCTGACGTGGCCAACCCATGCCGCCCCTTGGACCTGTGCATTGAATGGGCTGGGAGGAT
CTCTGAGGAGTATTTTGCACAGACTGATGAAGAGAAGAGACAGGGACTACCTGTGGTGATGCCAGTGTTTGACCGGAATACCTGTAGCATCCCCAAGTCT
CAGATCTCTTTCATTGACTACTTCATAACAGACATGTTTGATGCTTGGGATGCCTTTGCACATCTGCCAGCCCTGATGCAACATTTGGCTGACAACTACA
AACACTGGAAGACACTAGATGACCTAAAGTGCAAAAGTTTGAGGCTTCCATCTGACAGCTAAAGCCAAGCCACAGAGGGGGCCTCTTGACCGACAAAGGA
CACTGTGAATCACAGTAGCGTAAACGAGAGGCCTTCCTTTCTAATGACAATGACAGGTATTGGTGAAGGAGCTAATGTTTAATATTTGACCTTGAATCAT
TCAAGTCCCCAAATTTCATTCTTAGAAAGTTATGTTCCATGAAGAAAAATATATGTTCTTTTGAATACTTAATGACAGAACAAATACTTGGCAAACTCCT
TTGCTCTGCTGTCATCCTGTGTACCCTTGTCAATCCATGGAGCTGGTTCACTGTAACTAGCAGGCCACAGGAAGCAAAGCCTTGGTGCCTGTGAGCTCAT
CTCCCAGGATGGTGACTAAGTAGCTTAGCTAGTGATCAGCTCATCCTTTACCATAAAAGTCATCATTGCTGTTTAGCTTGACTGTTTTCCTCAAGAACAT
CGATCTGAAGGATTCATAAGGAGCTTATCTGAACAGATTTATCTAAGAAAAAAAAAAAACGACATAAAATAAGTGAAACAACTAGGACCAAATTACAGAT
AAACTAGTTAGCTTCACAGCCTCTATGGCTACATGGTTCTTCTGGCCGATGGTATGACACCTAAGTTAGAACACAGCCTTGGCTGGTGGGTGCCCTCTCT
AGACTGGTATCAGCAGCCTGTGTAACCCCTTTCCTGTAAAAGGGGTTCATCTTAACAAAGTCATCCATGATGAGGGAAAAAGTGGCATTTCATTTTTGGG
GAATCCATGAGCTTCCTTTATTTCTGGCTCACAGAGGCAGCCACGAGGCACTACACCAAGTATTATATAAAAGCCATTAAATTTGAATGCCCTTGGACAA
GCTTTTCTTAAAAAAAAAAAAAAAAA
  • refseq_PDE8B.F2 (forward primer)
  • refseq_PDE8B.R2 (reverse primer)
  • Single exon skipping

Frame 0

AAAGAGGRAGEPGRGMGCAPSIHVSQSGVIYCR
DSDESSSPRQTTSVSQGPAAPLPGLFVQTDAADA
IPPSRASGPPSVARVRRARTELGSGSSAGSAAP
AATTSRGRRRHCCSSAEAETQTCYTSVKQVSSA
EVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWAC
DRAGYRCNIARTPESALECFLDKHHEIIVIDHR
QTQNFDAEAVCRSIRATNPSEHTVILAVVSRVS
DDHEEASVLPLLHAGFNRRFMENSSIIACYNELI
QIEHGEVRSQFKLRACNSVFTALDHCHEAIEIT
SDDHVIQEWQGVYYARRKSGDSIQQHVKITPVI
GQGGKIRHFVSLKKLCCTTDNNKQIHKIHRDSGD
NSQTEPHSFRYKNRRKESIDVKSISSRGSDAPS
LQNRRYPSMARIHSMTIEAPITKVINIINAAQE
NSPVTVAEALDRVLEILRTTELYSPQLGTKDEDP
HTSDLVGGLMTDGLRRLSGNEYVFTKNVHQSHS
HLAMPITINDVPPCISQLLDNEESWDFNIFELE
AITHKRPLVYLGLKVFSRFGVCEFLNCSETTLRA
WFQVIEANYHSSNAYHNSTHAADVLHATAFFLG
KERVKGSLDQLDEVAALIAATVHDVDHPGRTNS
FLCNAGSELAVLYNDTAVLESHHTALAFQLTVKD
TKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKH
FEHVNKFVNSINKPMAAEIEGSDCECNPAGKNF
PENQILIKRMMIKCADVANPCRPLDLCIEWAGRI
SEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKS
QISFIDYFITDMFDAWDAFAHLPALMQHLADNY
KHWKTLDDLKCKSLRLPSDS*SQATEGAS*PTKD
TVNHSSVNERPSFLMTMTGIGEGANV*YLTLNH
SSPQISFLESYVP*RKIYVLLNT**QNKYLANS
FALLSSCVPLSIHGAGSL*LAGHRKQSLGACELI
SQDGD*VA*LVISSSFTIKVIIAV*LDCFPQEH
RSEGFIRSLSEQIYLRKKKNDIK*VKQLGPNYR
*TS*LHSLYGYMVLLADGMTPKLEHSLGWWVPSL
DWYQQPV*PLSCKRGSS*QSHP**GKKWHFIFG
ESMSFLYFWLTEAATRHYTKYYIKAIKFECPWT
SFS*KKKKK

Frame 1

RPRARAGARGSPAEGWAAPPASMSRRAA*STAG
TRTSPARPARPPACRRARRHPCPASSSRPTPPT
PSPRAARRDPPA*PASAGPAPSWAAVAARVPQPP
PRPPAGAGGATAAAAPRPRLRPATPA*SRCLLR
RCASGP*D*RRTLFRFC*SLQRKIVRAMASGGP
ATELVIDAILLGLQSQPLNAFLISIMKLL*LIID
KLRTSMQKQCAGRSGPQIPPSTR*SSQWFREYR
MTMKRRQSFLFSTQASTGDLWRIAA*LLAIMN*
FK*NMGKFAPSSNYGPVIQCLQH*ITVMKP*K*Q
AMTT*FRSGRGFTMPDGNPGTASNST*RSPQ*L
AKEGKLGILSRSRNCVVPLTIISRFTRFIVIQE
TILRQSLIHSDIRTGGKSPLT*NRYHLEAVMHQA
CRIVAIRPWRGSTP*PSRLPSQRL*I*SMQPKK
TAQSQ*RKPWTEF*RFYGPQNCTPLSWVPKMKI
LTPVILLEA**LTA*EDCQETSMCLLRMCTRVTV
TLQCQ*PSMMFPLVSLNYLIMRRVGTSTSLNWK
PLRIKGHWFIWA*RSSLGLEYVNF*TVLKPLFG
PGSK*SKPTTTLPMPTTTPPMLPTSCTPPLSFLE
RKE*REASISWMRWQPSLLPQSMTWITREGPTL
SSAMQAVSLLCSTMTLLFWRVTTPPWPSSSRSR
TPNATFSRILTGTIIERCARLLLTWFWQQR*QNT
LNM*ISL*TASTSQWQLRLKAATVNATLLGRTS
LKTKS*SNA**LSVLTWPTHAAPWTCALNGLGG
SLRSILHRLMKRRDRDYLW*CQCLTGIPVASPSL
RSLSLTTS*QTCLMLGMPLHICQP*CNIWLTTT
NTGRH*MT*SAKV*GFHLTAKAKPQRGPLDRQR
TL*ITVA*TRGLPF**Q*QVLVKELMFNI*P*II
QVPKFHS*KVMFHEEKYMFF*ILNDRTNTWQTP
LLCCHPVYPCQSMELVHCN*QATGSKALVPVSS
SPRMVTK*LS**SAHPLP*KSSLLFSLTVFLKNI
DLKDS*GAYLNRFI*EKKKTT*NK*NN*DQITD
KLVSFTASMATWFFWPMV*HLS*NTALAGGCPL
*TGISSLCNPFPVKGVHLNKVIHDEGKSGISFLG
NP*ASFISGSQRQPRGTTPSII*KPLNLNALGQ
AFLKKKKK

Frame 2

GRGRGRARGGARPRDGLRPQHPCLAERRDLLPG
LGRVQLAPPDHQRVAGPGGTPARPLRPDRRRRR
HPPEPRVGTPQRSPRPQGPHRAGQR*QRGFRSP
RRDHQQGPEAPLLQQRRGRDSDLLHQREAGVFCG
GAHRAHETDAGPYSGFADLCKGR*SERWLLVGL
RQSWL*MQYCSDSRVSP*MLS**AS*NYCN*S*
TNSELRCRSSVQVDPGHKSLRAHGDPRSGFASIG
*P*RGVSPSSSPRRLQQEIYGE*QHNCLL**TD
SNRTWGSSLPVQITGL*FSVYSIRSLS*SHRNN
KR*PRDSGVAGGLLCQTEIRGQHPTAREDHPSDW
PRREN*AFCLAQETVLYH*Q**ADSQDSS*FRR
QFSDRASFIQI*EQEERVH*REIDIISRQ*CTK
PAESSLSVHGEDPLHDHRGSHHKGYKYNQCSPRK
QPSHSSGSLGQSSRDFTDHRTVLPSAGYQR*RS
SHQ*SCWRPDD*RLEKTVRKRVCVY*ECAPESQ
SPCNANNHQ*CSPLYLSIT***GELGLQHL*IGS
HYA*KAIGLSGLKGLLSVWSM*IFKLF*NHSSG
LVPSDRSQLPLFQCLPQLHPCCRRPARHRFLSW
KGKSKGKPRSVG*GGSPHCCHSP*RGSPGKDQLF
PLQCRQ*ACCALQ*HCCSGESPHRPGLPAHGQG
HQMQHFQEY*QEPLSNAAPGYY*HGFGNRDDKT
L*TCE*VCEQHQQANGS*D*RQRL*MQPCWEELP
*KPNPDQTHDD*VC*RGQPMPPLGPVH*MGWED
L*GVFCTD**REETGTTCGDASV*PEYL*HPQV
SDLFH*LLHNRHV*CLGCLCTSASPDATFG*QLQ
TLEDTR*PKVQKFEASI*QLKPSHRGGLLTDKG
HCESQ*RKREAFLSNDNDRYW*RS*CLIFDLES
FKSPNFILRKLCSMKKNICSFEYLMTEQILGKLL
CSAVILCTLVNPWSWFTVTSRPQEAKPWCL*AH
LPGW*LSSLASDQLILYHKSHHCCLA*LFSSRT
SI*RIHKELI*TDLSKKKKKRHKISETTRTKLQI
N*LASQPLWLHGSSGRWYDT*VRTQPWLVGALS
RLVSAACVTPFL*KGFILTKSSMMREKVAFHFW
GIHELPLFLAHRGSHEALHQVLYKSH*I*MPLDK
LFLKKKKK

Sequence NM_001029853

GCGGCCGCGGGCGCGGGCGGGCGCGCGGGGGAGCCCGGCCGAGGGATGGGCTGCGCCCCCAGCATCCATGTCTCGCAGAGCGGCGTGATCTACTGCCGGG
ACTCGGACGAGTCCAGCTCGCCCCGCCAGACCACCAGCGTGTCGCAGGGCCCGGCGGCACCCCTGCCCGGCCTCTTCGTCCAGACCGACGCCGCCGACGC
CATCCCCCCGAGCCGCGCGTCGGGACCCCCCAGCGTAGCCCGCGTCCGCAGGGCCCGCACCGAGCTGGGCAGCGGTAGCAGCGCGGGTTCCGCAGCCCCC
GCCGCGACCACCAGCAGGGGCCGGAGGCGCCACTGCTGCAGCAGCGCCGAGGCCGAGACTCAGACCTGCTACACCAGCGTGAAGGTTTTGCTGATCTTTG
CAAAGGAAGATAGTCAGAGCGATGGCTTCTGGTGGGCCTGCGACAGAGCTGGTTATAGATGCAATATTGCTCGGACTCCAGAGTCAGCCCTTGAATGCTT
TCTTGATAAGCATCATGAAATTATTGTAATTGATCATAGACAAACTCAGAACTTCGATGCAGAAGCAGTGTGCAGGTCGATCCGGGCCACAAATCCCTCC
GAGCACACGGTGATCCTCGCAGTGGTTTCGCGAGTATCGGATGACCATGAAGAGGCGTCAGTCCTTCCTCTTCTCCACGCAGGCTTCAACAGGAGATTTA
TGGAGAATAGCAGCATAATTGCTTGCTATAATGAACTGATTCAAATAGAACATGGGGAAGTTCGCTCCCAGTTCAAATTACGGGCCTGTAATTCAGTGTT
TACAGCATTAGATCACTGTCATGAAGCCATAGAAATAACAAGCGATGACCACGTGATTCAGTATGTCAACCCAGCCTTCGAAAGGATGATGGGCTACCAC
AAAGGTGAGCTCCTGGGAAAAGAACTCGCTGATCTGCCCAAAAGCGATAAGAACCGGGCAGACCTTCTCGACACCATCAATACATGCATCAAGAAGGGAA
AGGAGTGGCAGGGGGTTTACTATGCCAGACGGAAATCCGGGGACAGCATCCAACAGCACGTGAAGATCACCCCAGTGATTGGCCAAGGAGGGAAAATTAG
GCATTTTGTCTCGCTCAAGAAACTGTGTTGTACCACTGACAATAATAAGCAGATTCACAAGATTCATCGTGATTCAGGAGACAATTCTCAGACAGAGCCT
CATTCATTCAGATATAAGAACAGGAGGAAAGAGTCCATTGACGTGAAATCGATATCATCTCGAGGCAGTGATGCACCAAGCCTGCAGAATCGTCGCTATC
CGTCCATGGCGAGGATCCACTCCATGACCATCGAGGCTCCCATCACAAAGGTTATAAATATAATCAATGCAGCCCAAGAAAACAGCCCAGTCACAGTAGC
GGAAGCCTTGGACAGAGTTCTAGAGATTTTACGGACCACAGAACTGTACTCCCCTCAGCTGGGTACCAAAGATGAAGATCCTCACACCAGTGATCTTGTT
GGAGGCCTGATGACTGACGGCTTGAGAAGACTGTCAGGAAACGAGTATGTGTTTACTAAGAATGTGCACCAGAGTCACAGTCACCTTGCAATGCCAATAA
CCATCAATGATGTTCCCCCTTGTATCTCTCAATTACTTGATAATGAGGAGAGTTGGGACTTCAACATCTTTGAATTGGAAGCCATTACGCATAAAAGGCC
ATTGGTTTATCTGGGCTTAAAGGTCTTCTCTCGGTTTGGAGTATGTGAATTTTTAAACTGTTCTGAAACCACTCTTCGGGCCTGGTTCCAAGTGATCGAA
GCCAACTACCACTCTTCCAATGCCTACCACAACTCCACCCATGCTGCCGACGTCCTGCACGCCACCGCTTTCTTTCTTGGAAAGGAAAGAGTAAAGGGAA
GCCTCGATCAGTTGGATGAGGTGGCAGCCCTCATTGCTGCCACAGTCCATGACGTGGATCACCCGGGAAGGACCAACTCTTTCCTCTGCAATGCAGGCAG
TGAGCTTGCTGTGCTCTACAATGACACTGCTGTTCTGGAGAGTCACCACACCGCCCTGGCCTTCCAGCTCACGGTCAAGGACACCAAATGCAACATTTTC
AAGAATATTGACAGGAACCATTATCGAACGCTGCGCCAGGCTATTATTGACATGGTTTTGGCAACAGAGATGACAAAACACTTTGAACATGTGAATAAGT
TTGTGAACAGCATCAACAAGCCAATGGCAGCTGAGATTGAAGGCAGCGACTGTGAATGCAACCCTGCTGGGAAGAACTTCCCTGAAAACCAAATCCTGAT
CAAACGCATGATGATTAAGTGTGCTGACGTGGCCAACCCATGCCGCCCCTTGGACCTGTGCATTGAATGGGCTGGGAGGATCTCTGAGGAGTATTTTGCA
CAGACTGATGAAGAGAAGAGACAGGGACTACCTGTGGTGATGCCAGTGTTTGACCGGAATACCTGTAGCATCCCCAAGTCTCAGATCTCTTTCATTGACT
ACTTCATAACAGACATGTTTGATGCTTGGGATGCCTTTGCACATCTGCCAGCCCTGATGCAACATTTGGCTGACAACTACAAACACTGGAAGACACTAGA
TGACCTAAAGTGCAAAAGTTTGAGGCTTCCATCTGACAGCTAAAGCCAAGCCACAGAGGGGGCCTCTTGACCGACAAAGGACACTGTGAATCACAGTAGC
GTAAACGAGAGGCCTTCCTTTCTAATGACAATGACAGGTATTGGTGAAGGAGCTAATGTTTAATATTTGACCTTGAATCATTCAAGTCCCCAAATTTCAT
TCTTAGAAAGTTATGTTCCATGAAGAAAAATATATGTTCTTTTGAATACTTAATGACAGAACAAATACTTGGCAAACTCCTTTGCTCTGCTGTCATCCTG
TGTACCCTTGTCAATCCATGGAGCTGGTTCACTGTAACTAGCAGGCCACAGGAAGCAAAGCCTTGGTGCCTGTGAGCTCATCTCCCAGGATGGTGACTAA
GTAGCTTAGCTAGTGATCAGCTCATCCTTTACCATAAAAGTCATCATTGCTGTTTAGCTTGACTGTTTTCCTCAAGAACATCGATCTGAAGGATTCATAA
GGAGCTTATCTGAACAGATTTATCTAAGAAAAAAAAAAAACGACATAAAATAAGTGAAACAACTAGGACCAAATTACAGATAAACTAGTTAGCTTCACAG
CCTCTATGGCTACATGGTTCTTCTGGCCGATGGTATGACACCTAAGTTAGAACACAGCCTTGGCTGGTGGGTGCCCTCTCTAGACTGGTATCAGCAGCCT
GTGTAACCCCTTTCCTGTAAAAGGGGTTCATCTTAACAAAGTCATCCATGATGAGGGAAAAAGTGGCATTTCATTTTTGGGGAATCCATGAGCTTCCTTT
ATTTCTGGCTCACAGAGGCAGCCACGAGGCACTACACCAAGTATTATATAAAAGCCATTAAATTTGAATGCCCTTGGACAAGCTTTTCTTAAAAAAAAAA
AAAAAAA
  • refseq_PDE8B.F2 (forward primer)
  • refseq_PDE8B.R2 (reverse primer)
  • Single exon skipping

Frame 0

AAAGAGGRAGEPGRGMGCAPSIHVSQSGVIYCR
DSDESSSPRQTTSVSQGPAAPLPGLFVQTDAADA
IPPSRASGPPSVARVRRARTELGSGSSAGSAAP
AATTSRGRRRHCCSSAEAETQTCYTSVKVLLIF
AKEDSQSDGFWWACDRAGYRCNIARTPESALECF
LDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPS
EHTVILAVVSRVSDDHEEASVLPLLHAGFNRRF
MENSSIIACYNELIQIEHGEVRSQFKLRACNSVF
TALDHCHEAIEITSDDHVIQYVNPAFERMMGYH
KGELLGKELADLPKSDKNRADLLDTINTCIKKG
KEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIR
HFVSLKKLCCTTDNNKQIHKIHRDSGDNSQTEP
HSFRYKNRRKESIDVKSISSRGSDAPSLQNRRY
PSMARIHSMTIEAPITKVINIINAAQENSPVTVA
EALDRVLEILRTTELYSPQLGTKDEDPHTSDLV
GGLMTDGLRRLSGNEYVFTKNVHQSHSHLAMPI
TINDVPPCISQLLDNEESWDFNIFELEAITHKRP
LVYLGLKVFSRFGVCEFLNCSETTLRAWFQVIE
ANYHSSNAYHNSTHAADVLHATAFFLGKERVKG
SLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGS
ELAVLYNDTAVLESHHTALAFQLTVKDTKCNIF
KNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNK
FVNSINKPMAAEIEGSDCECNPAGKNFPENQILI
KRMMIKCADVANPCRPLDLCIEWAGRISEEYFA
QTDEEKRQGLPVVMPVFDRNTCSIPKSQISFID
YFITDMFDAWDAFAHLPALMQHLADNYKHWKTLD
DLKCKSLRLPSDS*SQATEGAS*PTKDTVNHSS
VNERPSFLMTMTGIGEGANV*YLTLNHSSPQIS
FLESYVP*RKIYVLLNT**QNKYLANSFALLSSC
VPLSIHGAGSL*LAGHRKQSLGACELISQDGD*
VA*LVISSSFTIKVIIAV*LDCFPQEHRSEGFI
RSLSEQIYLRKKKNDIK*VKQLGPNYR*TS*LHS
LYGYMVLLADGMTPKLEHSLGWWVPSLDWYQQP
V*PLSCKRGSS*QSHP**GKKWHFIFGESMSFL
YFWLTEAATRHYTKYYIKAIKFECPWTSFS*KKK
KK

Frame 1

RPRARAGARGSPAEGWAAPPASMSRRAA*STAG
TRTSPARPARPPACRRARRHPCPASSSRPTPPT
PSPRAARRDPPA*PASAGPAPSWAAVAARVPQPP
PRPPAGAGGATAAAAPRPRLRPATPA*RFC*SL
QRKIVRAMASGGPATELVIDAILLGLQSQPLNA
FLISIMKLL*LIIDKLRTSMQKQCAGRSGPQIPP
STR*SSQWFREYRMTMKRRQSFLFSTQASTGDL
WRIAA*LLAIMN*FK*NMGKFAPSSNYGPVIQC
LQH*ITVMKP*K*QAMTT*FSMSTQPSKG*WATT
KVSSWEKNSLICPKAIRTGQTFSTPSIHASRRE
RSGRGFTMPDGNPGTASNST*RSPQ*LAKEGKL
GILSRSRNCVVPLTIISRFTRFIVIQETILRQSL
IHSDIRTGGKSPLT*NRYHLEAVMHQACRIVAI
RPWRGSTP*PSRLPSQRL*I*SMQPKKTAQSQ*
RKPWTEF*RFYGPQNCTPLSWVPKMKILTPVILL
EA**LTA*EDCQETSMCLLRMCTRVTVTLQCQ*
PSMMFPLVSLNYLIMRRVGTSTSLNWKPLRIKG
HWFIWA*RSSLGLEYVNF*TVLKPLFGPGSK*SK
PTTTLPMPTTTPPMLPTSCTPPLSFLERKE*RE
ASISWMRWQPSLLPQSMTWITREGPTLSSAMQA
VSLLCSTMTLLFWRVTTPPWPSSSRSRTPNATFS
RILTGTIIERCARLLLTWFWQQR*QNTLNM*IS
L*TASTSQWQLRLKAATVNATLLGRTSLKTKS*
SNA**LSVLTWPTHAAPWTCALNGLGGSLRSILH
RLMKRRDRDYLW*CQCLTGIPVASPSLRSLSLT
TS*QTCLMLGMPLHICQP*CNIWLTTTNTGRH*
MT*SAKV*GFHLTAKAKPQRGPLDRQRTL*ITVA
*TRGLPF**Q*QVLVKELMFNI*P*IIQVPKFH
S*KVMFHEEKYMFF*ILNDRTNTWQTPLLCCHP
VYPCQSMELVHCN*QATGSKALVPVSSSPRMVTK
*LS**SAHPLP*KSSLLFSLTVFLKNIDLKDS*
GAYLNRFI*EKKKTT*NK*NN*DQITDKLVSFT
ASMATWFFWPMV*HLS*NTALAGGCPL*TGISSL
CNPFPVKGVHLNKVIHDEGKSGISFLGNP*ASF
ISGSQRQPRGTTPSII*KPLNLNALGQAFLKKK
KK

Frame 2

GRGRGRARGGARPRDGLRPQHPCLAERRDLLPG
LGRVQLAPPDHQRVAGPGGTPARPLRPDRRRRR
HPPEPRVGTPQRSPRPQGPHRAGQR*QRGFRSP
RRDHQQGPEAPLLQQRRGRDSDLLHQREGFADLC
KGR*SERWLLVGLRQSWL*MQYCSDSRVSP*ML
S**AS*NYCN*S*TNSELRCRSSVQVDPGHKSL
RAHGDPRSGFASIG*P*RGVSPSSSPRRLQQEIY
GE*QHNCLL**TDSNRTWGSSLPVQITGL*FSV
YSIRSLS*SHRNNKR*PRDSVCQPSLRKDDGLP
QR*APGKRTR*SAQKR*EPGRPSRHHQYMHQEGK
GVAGGLLCQTEIRGQHPTAREDHPSDWPRREN*
AFCLAQETVLYH*Q**ADSQDSS*FRRQFSDRA
SFIQI*EQEERVH*REIDIISRQ*CTKPAESSLS
VHGEDPLHDHRGSHHKGYKYNQCSPRKQPSHSS
GSLGQSSRDFTDHRTVLPSAGYQR*RSSHQ*SC
WRPDD*RLEKTVRKRVCVY*ECAPESQSPCNANN
HQ*CSPLYLSIT***GELGLQHL*IGSHYA*KA
IGLSGLKGLLSVWSM*IFKLF*NHSSGLVPSDR
SQLPLFQCLPQLHPCCRRPARHRFLSWKGKSKGK
PRSVG*GGSPHCCHSP*RGSPGKDQLFPLQCRQ
*ACCALQ*HCCSGESPHRPGLPAHGQGHQMQHF
QEY*QEPLSNAAPGYY*HGFGNRDDKTL*TCE*V
CEQHQQANGS*D*RQRL*MQPCWEELP*KPNPD
QTHDD*VC*RGQPMPPLGPVH*MGWEDL*GVFC
TD**REETGTTCGDASV*PEYL*HPQVSDLFH*L
LHNRHV*CLGCLCTSASPDATFG*QLQTLEDTR
*PKVQKFEASI*QLKPSHRGGLLTDKGHCESQ*
RKREAFLSNDNDRYW*RS*CLIFDLESFKSPNFI
LRKLCSMKKNICSFEYLMTEQILGKLLCSAVIL
CTLVNPWSWFTVTSRPQEAKPWCL*AHLPGW*L
SSLASDQLILYHKSHHCCLA*LFSSRTSI*RIHK
ELI*TDLSKKKKKRHKISETTRTKLQIN*LASQ
PLWLHGSSGRWYDT*VRTQPWLVGALSRLVSAA
CVTPFL*KGFILTKSSMMREKVAFHFWGIHELPL
FLAHRGSHEALHQVLYKSH*I*MPLDKLFLKKK
KK

Sequence NM_003719

GCGGCCGCGGGCGCGGGCGGGCGCGCGGGGGAGCCCGGCCGAGGGATGGGCTGCGCCCCCAGCATCCATGTCTCGCAGAGCGGCGTGATCTACTGCCGGG
ACTCGGACGAGTCCAGCTCGCCCCGCCAGACCACCAGCGTGTCGCAGGGCCCGGCGGCACCCCTGCCCGGCCTCTTCGTCCAGACCGACGCCGCCGACGC
CATCCCCCCGAGCCGCGCGTCGGGACCCCCCAGCGTAGCCCGCGTCCGCAGGGCCCGCACCGAGCTGGGCAGCGGTAGCAGCGCGGGTTCCGCAGCCCCC
GCCGCGACCACCAGCAGGGGCCGGAGGCGCCACTGCTGCAGCAGCGCCGAGGCCGAGACTCAGACCTGCTACACCAGCGTGAAGCAGGTGTCTTCTGCGG
AGGTGCGCATCGGGCCCATGAGACTGACGCAGGACCCTATTCAGGTTTTGCTGATCTTTGCAAAGGAAGATAGTCAGAGCGATGGCTTCTGGTGGGCCTG
CGACAGAGCTGGTTATAGATGCAATATTGCTCGGACTCCAGAGTCAGCCCTTGAATGCTTTCTTGATAAGCATCATGAAATTATTGTAATTGATCATAGA
CAAACTCAGAACTTCGATGCAGAAGCAGTGTGCAGGTCGATCCGGGCCACAAATCCCTCCGAGCACACGGTGATCCTCGCAGTGGTTTCGCGAGTATCGG
ATGACCATGAAGAGGCGTCAGTCCTTCCTCTTCTCCACGCAGGCTTCAACAGGAGATTTATGGAGAATAGCAGCATAATTGCTTGCTATAATGAACTGAT
TCAAATAGAACATGGGGAAGTTCGCTCCCAGTTCAAATTACGGGCCTGTAATTCAGTGTTTACAGCATTAGATCACTGTCATGAAGCCATAGAAATAACA
AGCGATGACCACGTGATTCAGTATGTCAACCCAGCCTTCGAAAGGATGATGGGCTACCACAAAGGTGAGCTCCTGGGAAAAGAACTCGCTGATCTGCCCA
AAAGCGATAAGAACCGGGCAGACCTTCTCGACACCATCAATACATGCATCAAGAAGGGAAAGGAGTGGCAGGGGGTTTACTATGCCAGACGGAAATCCGG
GGACAGCATCCAACAGCACGTGAAGATCACCCCAGTGATTGGCCAAGGAGGGAAAATTAGGCATTTTGTCTCGCTCAAGAAACTGTGTTGTACCACTGAC
AATAATAAGCAGATTCACAAGATTCATCGTGATTCAGGAGACAATTCTCAGACAGAGCCTCATTCATTCAGATATAAGAACAGGAGGAAAGAGTCCATTG
ACGTGAAATCGATATCATCTCGAGGCAGTGATGCACCAAGCCTGCAGAATCGTCGCTATCCGTCCATGGCGAGGATCCACTCCATGACCATCGAGGCTCC
CATCACAAAGGTTATAAATATAATCAATGCAGCCCAAGAAAACAGCCCAGTCACAGTAGCGGAAGCCTTGGACAGAGTTCTAGAGATTTTACGGACCACA
GAACTGTACTCCCCTCAGCTGGGTACCAAAGATGAAGATCCTCACACCAGTGATCTTGTTGGAGGCCTGATGACTGACGGCTTGAGAAGACTGTCAGGAA
ACGAGTATGTGTTTACTAAGAATGTGCACCAGAGTCACAGTCACCTTGCAATGCCAATAACCATCAATGATGTTCCCCCTTGTATCTCTCAATTACTTGA
TAATGAGGAGAGTTGGGACTTCAACATCTTTGAATTGGAAGCCATTACGCATAAAAGGCCATTGGTTTATCTGGGCTTAAAGGTCTTCTCTCGGTTTGGA
GTATGTGAATTTTTAAACTGTTCTGAAACCACTCTTCGGGCCTGGTTCCAAGTGATCGAAGCCAACTACCACTCTTCCAATGCCTACCACAACTCCACCC
ATGCTGCCGACGTCCTGCACGCCACCGCTTTCTTTCTTGGAAAGGAAAGAGTAAAGGGAAGCCTCGATCAGTTGGATGAGGTGGCAGCCCTCATTGCTGC
CACAGTCCATGACGTGGATCACCCGGGAAGGACCAACTCTTTCCTCTGCAATGCAGGCAGTGAGCTTGCTGTGCTCTACAATGACACTGCTGTTCTGGAG
AGTCACCACACCGCCCTGGCCTTCCAGCTCACGGTCAAGGACACCAAATGCAACATTTTCAAGAATATTGACAGGAACCATTATCGAACGCTGCGCCAGG
CTATTATTGACATGGTTTTGGCAACAGAGATGACAAAACACTTTGAACATGTGAATAAGTTTGTGAACAGCATCAACAAGCCAATGGCAGCTGAGATTGA
AGGCAGCGACTGTGAATGCAACCCTGCTGGGAAGAACTTCCCTGAAAACCAAATCCTGATCAAACGCATGATGATTAAGTGTGCTGACGTGGCCAACCCA
TGCCGCCCCTTGGACCTGTGCATTGAATGGGCTGGGAGGATCTCTGAGGAGTATTTTGCACAGACTGATGAAGAGAAGAGACAGGGACTACCTGTGGTGA
TGCCAGTGTTTGACCGGAATACCTGTAGCATCCCCAAGTCTCAGATCTCTTTCATTGACTACTTCATAACAGACATGTTTGATGCTTGGGATGCCTTTGC
ACATCTGCCAGCCCTGATGCAACATTTGGCTGACAACTACAAACACTGGAAGACACTAGATGACCTAAAGTGCAAAAGTTTGAGGCTTCCATCTGACAGC
TAAAGCCAAGCCACAGAGGGGGCCTCTTGACCGACAAAGGACACTGTGAATCACAGTAGCGTAAACGAGAGGCCTTCCTTTCTAATGACAATGACAGGTA
TTGGTGAAGGAGCTAATGTTTAATATTTGACCTTGAATCATTCAAGTCCCCAAATTTCATTCTTAGAAAGTTATGTTCCATGAAGAAAAATATATGTTCT
TTTGAATACTTAATGACAGAACAAATACTTGGCAAACTCCTTTGCTCTGCTGTCATCCTGTGTACCCTTGTCAATCCATGGAGCTGGTTCACTGTAACTA
GCAGGCCACAGGAAGCAAAGCCTTGGTGCCTGTGAGCTCATCTCCCAGGATGGTGACTAAGTAGCTTAGCTAGTGATCAGCTCATCCTTTACCATAAAAG
TCATCATTGCTGTTTAGCTTGACTGTTTTCCTCAAGAACATCGATCTGAAGGATTCATAAGGAGCTTATCTGAACAGATTTATCTAAGAAAAAAAAAAAA
CGACATAAAATAAGTGAAACAACTAGGACCAAATTACAGATAAACTAGTTAGCTTCACAGCCTCTATGGCTACATGGTTCTTCTGGCCGATGGTATGACA
CCTAAGTTAGAACACAGCCTTGGCTGGTGGGTGCCCTCTCTAGACTGGTATCAGCAGCCTGTGTAACCCCTTTCCTGTAAAAGGGGTTCATCTTAACAAA
GTCATCCATGATGAGGGAAAAAGTGGCATTTCATTTTTGGGGAATCCATGAGCTTCCTTTATTTCTGGCTCACAGAGGCAGCCACGAGGCACTACACCAA
GTATTATATAAAAGCCATTAAATTTGAATGCCCTTGGACAAGCTTTTCTTAAAAAAAAAAAAAAAAA
  • refseq_PDE8B.F2 (forward primer)
  • refseq_PDE8B.R2 (reverse primer)
  • Single exon skipping

Frame 0

AAAGAGGRAGEPGRGMGCAPSIHVSQSGVIYCR
DSDESSSPRQTTSVSQGPAAPLPGLFVQTDAADA
IPPSRASGPPSVARVRRARTELGSGSSAGSAAP
AATTSRGRRRHCCSSAEAETQTCYTSVKQVSSA
EVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWAC
DRAGYRCNIARTPESALECFLDKHHEIIVIDHR
QTQNFDAEAVCRSIRATNPSEHTVILAVVSRVS
DDHEEASVLPLLHAGFNRRFMENSSIIACYNELI
QIEHGEVRSQFKLRACNSVFTALDHCHEAIEIT
SDDHVIQYVNPAFERMMGYHKGELLGKELADLP
KSDKNRADLLDTINTCIKKGKEWQGVYYARRKSG
DSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTD
NNKQIHKIHRDSGDNSQTEPHSFRYKNRRKESI
DVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAP
ITKVINIINAAQENSPVTVAEALDRVLEILRTT
ELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSG
NEYVFTKNVHQSHSHLAMPITINDVPPCISQLLD
NEESWDFNIFELEAITHKRPLVYLGLKVFSRFG
VCEFLNCSETTLRAWFQVIEANYHSSNAYHNST
HAADVLHATAFFLGKERVKGSLDQLDEVAALIAA
TVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLE
SHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQ
AIIDMVLATEMTKHFEHVNKFVNSINKPMAAEIE
GSDCECNPAGKNFPENQILIKRMMIKCADVANP
CRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVV
MPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFA
HLPALMQHLADNYKHWKTLDDLKCKSLRLPSDS
*SQATEGAS*PTKDTVNHSSVNERPSFLMTMTG
IGEGANV*YLTLNHSSPQISFLESYVP*RKIYVL
LNT**QNKYLANSFALLSSCVPLSIHGAGSL*L
AGHRKQSLGACELISQDGD*VA*LVISSSFTIK
VIIAV*LDCFPQEHRSEGFIRSLSEQIYLRKKKN
DIK*VKQLGPNYR*TS*LHSLYGYMVLLADGMT
PKLEHSLGWWVPSLDWYQQPV*PLSCKRGSS*Q
SHP**GKKWHFIFGESMSFLYFWLTEAATRHYTK
YYIKAIKFECPWTSFS*KKKKK

Frame 1

RPRARAGARGSPAEGWAAPPASMSRRAA*STAG
TRTSPARPARPPACRRARRHPCPASSSRPTPPT
PSPRAARRDPPA*PASAGPAPSWAAVAARVPQPP
PRPPAGAGGATAAAAPRPRLRPATPA*SRCLLR
RCASGP*D*RRTLFRFC*SLQRKIVRAMASGGP
ATELVIDAILLGLQSQPLNAFLISIMKLL*LIID
KLRTSMQKQCAGRSGPQIPPSTR*SSQWFREYR
MTMKRRQSFLFSTQASTGDLWRIAA*LLAIMN*
FK*NMGKFAPSSNYGPVIQCLQH*ITVMKP*K*Q
AMTT*FSMSTQPSKG*WATTKVSSWEKNSLICP
KAIRTGQTFSTPSIHASRRERSGRGFTMPDGNP
GTASNST*RSPQ*LAKEGKLGILSRSRNCVVPLT
IISRFTRFIVIQETILRQSLIHSDIRTGGKSPL
T*NRYHLEAVMHQACRIVAIRPWRGSTP*PSRL
PSQRL*I*SMQPKKTAQSQ*RKPWTEF*RFYGPQ
NCTPLSWVPKMKILTPVILLEA**LTA*EDCQE
TSMCLLRMCTRVTVTLQCQ*PSMMFPLVSLNYL
IMRRVGTSTSLNWKPLRIKGHWFIWA*RSSLGLE
YVNF*TVLKPLFGPGSK*SKPTTTLPMPTTTPP
MLPTSCTPPLSFLERKE*REASISWMRWQPSLL
PQSMTWITREGPTLSSAMQAVSLLCSTMTLLFWR
VTTPPWPSSSRSRTPNATFSRILTGTIIERCAR
LLLTWFWQQR*QNTLNM*ISL*TASTSQWQLRL
KAATVNATLLGRTSLKTKS*SNA**LSVLTWPTH
AAPWTCALNGLGGSLRSILHRLMKRRDRDYLW*
CQCLTGIPVASPSLRSLSLTTS*QTCLMLGMPL
HICQP*CNIWLTTTNTGRH*MT*SAKV*GFHLTA
KAKPQRGPLDRQRTL*ITVA*TRGLPF**Q*QV
LVKELMFNI*P*IIQVPKFHS*KVMFHEEKYMF
F*ILNDRTNTWQTPLLCCHPVYPCQSMELVHCN*
QATGSKALVPVSSSPRMVTK*LS**SAHPLP*K
SSLLFSLTVFLKNIDLKDS*GAYLNRFI*EKKK
TT*NK*NN*DQITDKLVSFTASMATWFFWPMV*H
LS*NTALAGGCPL*TGISSLCNPFPVKGVHLNK
VIHDEGKSGISFLGNP*ASFISGSQRQPRGTTP
SII*KPLNLNALGQAFLKKKKK

Frame 2

GRGRGRARGGARPRDGLRPQHPCLAERRDLLPG
LGRVQLAPPDHQRVAGPGGTPARPLRPDRRRRR
HPPEPRVGTPQRSPRPQGPHRAGQR*QRGFRSP
RRDHQQGPEAPLLQQRRGRDSDLLHQREAGVFCG
GAHRAHETDAGPYSGFADLCKGR*SERWLLVGL
RQSWL*MQYCSDSRVSP*MLS**AS*NYCN*S*
TNSELRCRSSVQVDPGHKSLRAHGDPRSGFASIG
*P*RGVSPSSSPRRLQQEIYGE*QHNCLL**TD
SNRTWGSSLPVQITGL*FSVYSIRSLS*SHRNN
KR*PRDSVCQPSLRKDDGLPQR*APGKRTR*SAQ
KR*EPGRPSRHHQYMHQEGKGVAGGLLCQTEIR
GQHPTAREDHPSDWPRREN*AFCLAQETVLYH*
Q**ADSQDSS*FRRQFSDRASFIQI*EQEERVH*
REIDIISRQ*CTKPAESSLSVHGEDPLHDHRGS
HHKGYKYNQCSPRKQPSHSSGSLGQSSRDFTDH
RTVLPSAGYQR*RSSHQ*SCWRPDD*RLEKTVRK
RVCVY*ECAPESQSPCNANNHQ*CSPLYLSIT*
**GELGLQHL*IGSHYA*KAIGLSGLKGLLSVW
SM*IFKLF*NHSSGLVPSDRSQLPLFQCLPQLHP
CCRRPARHRFLSWKGKSKGKPRSVG*GGSPHCC
HSP*RGSPGKDQLFPLQCRQ*ACCALQ*HCCSG
ESPHRPGLPAHGQGHQMQHFQEY*QEPLSNAAPG
YY*HGFGNRDDKTL*TCE*VCEQHQQANGS*D*
RQRL*MQPCWEELP*KPNPDQTHDD*VC*RGQP
MPPLGPVH*MGWEDL*GVFCTD**REETGTTCGD
ASV*PEYL*HPQVSDLFH*LLHNRHV*CLGCLC
TSASPDATFG*QLQTLEDTR*PKVQKFEASI*Q
LKPSHRGGLLTDKGHCESQ*RKREAFLSNDNDRY
W*RS*CLIFDLESFKSPNFILRKLCSMKKNICS
FEYLMTEQILGKLLCSAVILCTLVNPWSWFTVT
SRPQEAKPWCL*AHLPGW*LSSLASDQLILYHKS
HHCCLA*LFSSRTSI*RIHKELI*TDLSKKKKK
RHKISETTRTKLQIN*LASQPLWLHGSSGRWYD
T*VRTQPWLVGALSRLVSAACVTPFL*KGFILTK
SSMMREKVAFHFWGIHELPLFLAHRGSHEALHQ
VLYKSH*I*MPLDKLFLKKKKK

Sequence NM_001029852

GCGGCCGCGGGCGCGGGCGGGCGCGCGGGGGAGCCCGGCCGAGGGATGGGCTGCGCCCCCAGCATCCATGTCTCGCAGAGCGGCGTGATCTACTGCCGGG
ACTCGGACGAGTCCAGCTCGCCCCGCCAGACCACCAGCGTGTCGCAGGGCCCGGCGGCACCCCTGCCCGGCCTCTTCGTCCAGACCGACGCCGCCGACGC
CATCCCCCCGAGCCGCGCGTCGGGACCCCCCAGCGTAGCCCGCGTCCGCAGGGCCCGCACCGAGCTGGGCAGCGGTAGCAGCGCGGGTTCCGCAGCCCCC
GCCGCGACCACCAGCAGGGGCCGGAGGCGCCACTGCTGCAGCAGCGCCGAGGCCGAGACTCAGACCTGCTACACCAGCGTGAAGCAGGTGTCTTCTGCGG
AGGTGCGCATCGGGCCCATGAGACTGACGCAGGACCCTATTCAGGTTTTGCTGATCTTTGCAAAGGAAGATAGTCAGAGCGATGGCTTCTGGTGGGCCTG
CGACAGAGCTGGTTATAGATGCAATATTGCTCGGACTCCAGAGTCAGCCCTTGAATGCTTTCTTGATAAGCATCATGAAATTATTGTAATTGATCATAGA
CAAACTCAGAACTTCGATGCAGAAGCAGTGTGCAGGTCGATCCGGGCCACAAATCCCTCCGAGCACACGGTGATCCTCGCAGTGGTTTCGCGAGTATCGG
ATGACCATGAAGAGGCGTCAGTCCTTCCTCTTCTCCACGCAGGCTTCAACAGGAGATTTATGGAGAATAGCAGCATAATTGCTTGCTATAATGAACTGAT
TCAAATAGAACATGGGGAAGTTCGCTCCCAGTTCAAATTACGGGCCTGTAATTCAGTGTTTACAGCATTAGATCACTGTCATGAAGCCATAGAAATAACA
AGCGATGACCACGTGATTCAGTATGTCAACCCAGCCTTCGAAAGGATGATGGGCTACCACAAAGGTGAGCTCCTGGGAAAAGAACTCGCTGATCTGCCCA
AAAGCGATAAGAACCGGGCAGACCTTCTCGACACCATCAATACATGCATCAAGAAGGGAAAGGAGTGGCAGGGGGTTTACTATGCCAGACGGAAATCCGG
GGACAGCATCCAACAGCACGTGAAGATCACCCCAGTGATTGGCCAAGGAGGGAAAATTAGGCATTTTGTCTCGCTCAAGAAACTGTGTTGTACCACTGAC
AATAATAAGCAGATTCACAAGATTCATCGTGATTCAGGAGACAATTCTCAGACAGAGCCTCATTCATTCAGATATAAGAACAGGAGGAAAGAGTCCATTG
ACGTGAAATCGATATCATCTCGAGGCAGTGATGCACCAAGCCTGCAGAATCGTCGCTATCCGTCCATGGCGAGGATCCACTCCATGACCATCGAGGCTCC
CATCACAAAGGACGGCTTGAGAAGACTGTCAGGAAACGAGTATGTGTTTACTAAGAATGTGCACCAGAGTCACAGTCACCTTGCAATGCCAATAACCATC
AATGATGTTCCCCCTTGTATCTCTCAATTACTTGATAATGAGGAGAGTTGGGACTTCAACATCTTTGAATTGGAAGCCATTACGCATAAAAGGCCATTGG
TTTATCTGGGCTTAAAGGTCTTCTCTCGGTTTGGAGTATGTGAATTTTTAAACTGTTCTGAAACCACTCTTCGGGCCTGGTTCCAAGTGATCGAAGCCAA
CTACCACTCTTCCAATGCCTACCACAACTCCACCCATGCTGCCGACGTCCTGCACGCCACCGCTTTCTTTCTTGGAAAGGAAAGAGTAAAGGGAAGCCTC
GATCAGTTGGATGAGGTGGCAGCCCTCATTGCTGCCACAGTCCATGACGTGGATCACCCGGGAAGGACCAACTCTTTCCTCTGCAATGCAGGCAGTGAGC
TTGCTGTGCTCTACAATGACACTGCTGTTCTGGAGAGTCACCACACCGCCCTGGCCTTCCAGCTCACGGTCAAGGACACCAAATGCAACATTTTCAAGAA
TATTGACAGGAACCATTATCGAACGCTGCGCCAGGCTATTATTGACATGGTTTTGGCAACAGAGATGACAAAACACTTTGAACATGTGAATAAGTTTGTG
AACAGCATCAACAAGCCAATGGCAGCTGAGATTGAAGGCAGCGACTGTGAATGCAACCCTGCTGGGAAGAACTTCCCTGAAAACCAAATCCTGATCAAAC
GCATGATGATTAAGTGTGCTGACGTGGCCAACCCATGCCGCCCCTTGGACCTGTGCATTGAATGGGCTGGGAGGATCTCTGAGGAGTATTTTGCACAGAC
TGATGAAGAGAAGAGACAGGGACTACCTGTGGTGATGCCAGTGTTTGACCGGAATACCTGTAGCATCCCCAAGTCTCAGATCTCTTTCATTGACTACTTC
ATAACAGACATGTTTGATGCTTGGGATGCCTTTGCACATCTGCCAGCCCTGATGCAACATTTGGCTGACAACTACAAACACTGGAAGACACTAGATGACC
TAAAGTGCAAAAGTTTGAGGCTTCCATCTGACAGCTAAAGCCAAGCCACAGAGGGGGCCTCTTGACCGACAAAGGACACTGTGAATCACAGTAGCGTAAA
CGAGAGGCCTTCCTTTCTAATGACAATGACAGGTATTGGTGAAGGAGCTAATGTTTAATATTTGACCTTGAATCATTCAAGTCCCCAAATTTCATTCTTA
GAAAGTTATGTTCCATGAAGAAAAATATATGTTCTTTTGAATACTTAATGACAGAACAAATACTTGGCAAACTCCTTTGCTCTGCTGTCATCCTGTGTAC
CCTTGTCAATCCATGGAGCTGGTTCACTGTAACTAGCAGGCCACAGGAAGCAAAGCCTTGGTGCCTGTGAGCTCATCTCCCAGGATGGTGACTAAGTAGC
TTAGCTAGTGATCAGCTCATCCTTTACCATAAAAGTCATCATTGCTGTTTAGCTTGACTGTTTTCCTCAAGAACATCGATCTGAAGGATTCATAAGGAGC
TTATCTGAACAGATTTATCTAAGAAAAAAAAAAAACGACATAAAATAAGTGAAACAACTAGGACCAAATTACAGATAAACTAGTTAGCTTCACAGCCTCT
ATGGCTACATGGTTCTTCTGGCCGATGGTATGACACCTAAGTTAGAACACAGCCTTGGCTGGTGGGTGCCCTCTCTAGACTGGTATCAGCAGCCTGTGTA
ACCCCTTTCCTGTAAAAGGGGTTCATCTTAACAAAGTCATCCATGATGAGGGAAAAAGTGGCATTTCATTTTTGGGGAATCCATGAGCTTCCTTTATTTC
TGGCTCACAGAGGCAGCCACGAGGCACTACACCAAGTATTATATAAAAGCCATTAAATTTGAATGCCCTTGGACAAGCTTTTCTTAAAAAAAAAAAAAAA
AA
  • refseq_PDE8B.F2 (forward primer)
  • refseq_PDE8B.R2 (reverse primer)
  • Single exon skipping

Frame 0

AAAGAGGRAGEPGRGMGCAPSIHVSQSGVIYCR
DSDESSSPRQTTSVSQGPAAPLPGLFVQTDAADA
IPPSRASGPPSVARVRRARTELGSGSSAGSAAP
AATTSRGRRRHCCSSAEAETQTCYTSVKQVSSA
EVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWAC
DRAGYRCNIARTPESALECFLDKHHEIIVIDHR
QTQNFDAEAVCRSIRATNPSEHTVILAVVSRVS
DDHEEASVLPLLHAGFNRRFMENSSIIACYNELI
QIEHGEVRSQFKLRACNSVFTALDHCHEAIEIT
SDDHVIQYVNPAFERMMGYHKGELLGKELADLP
KSDKNRADLLDTINTCIKKGKEWQGVYYARRKSG
DSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTD
NNKQIHKIHRDSGDNSQTEPHSFRYKNRRKESI
DVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAP
ITKDGLRRLSGNEYVFTKNVHQSHSHLAMPITI
NDVPPCISQLLDNEESWDFNIFELEAITHKRPL
VYLGLKVFSRFGVCEFLNCSETTLRAWFQVIEAN
YHSSNAYHNSTHAADVLHATAFFLGKERVKGSL
DQLDEVAALIAATVHDVDHPGRTNSFLCNAGSE
LAVLYNDTAVLESHHTALAFQLTVKDTKCNIFKN
IDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFV
NSINKPMAAEIEGSDCECNPAGKNFPENQILIK
RMMIKCADVANPCRPLDLCIEWAGRISEEYFAQT
DEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYF
ITDMFDAWDAFAHLPALMQHLADNYKHWKTLDD
LKCKSLRLPSDS*SQATEGAS*PTKDTVNHSSVN
ERPSFLMTMTGIGEGANV*YLTLNHSSPQISFL
ESYVP*RKIYVLLNT**QNKYLANSFALLSSCV
PLSIHGAGSL*LAGHRKQSLGACELISQDGD*VA
*LVISSSFTIKVIIAV*LDCFPQEHRSEGFIRS
LSEQIYLRKKKNDIK*VKQLGPNYR*TS*LHSL
YGYMVLLADGMTPKLEHSLGWWVPSLDWYQQPV*
PLSCKRGSS*QSHP**GKKWHFIFGESMSFLYF
WLTEAATRHYTKYYIKAIKFECPWTSFS*KKKK
K

Frame 1

RPRARAGARGSPAEGWAAPPASMSRRAA*STAG
TRTSPARPARPPACRRARRHPCPASSSRPTPPT
PSPRAARRDPPA*PASAGPAPSWAAVAARVPQPP
PRPPAGAGGATAAAAPRPRLRPATPA*SRCLLR
RCASGP*D*RRTLFRFC*SLQRKIVRAMASGGP
ATELVIDAILLGLQSQPLNAFLISIMKLL*LIID
KLRTSMQKQCAGRSGPQIPPSTR*SSQWFREYR
MTMKRRQSFLFSTQASTGDLWRIAA*LLAIMN*
FK*NMGKFAPSSNYGPVIQCLQH*ITVMKP*K*Q
AMTT*FSMSTQPSKG*WATTKVSSWEKNSLICP
KAIRTGQTFSTPSIHASRRERSGRGFTMPDGNP
GTASNST*RSPQ*LAKEGKLGILSRSRNCVVPLT
IISRFTRFIVIQETILRQSLIHSDIRTGGKSPL
T*NRYHLEAVMHQACRIVAIRPWRGSTP*PSRL
PSQRTA*EDCQETSMCLLRMCTRVTVTLQCQ*PS
MMFPLVSLNYLIMRRVGTSTSLNWKPLRIKGHW
FIWA*RSSLGLEYVNF*TVLKPLFGPGSK*SKP
TTTLPMPTTTPPMLPTSCTPPLSFLERKE*REAS
ISWMRWQPSLLPQSMTWITREGPTLSSAMQAVS
LLCSTMTLLFWRVTTPPWPSSSRSRTPNATFSR
ILTGTIIERCARLLLTWFWQQR*QNTLNM*ISL*
TASTSQWQLRLKAATVNATLLGRTSLKTKS*SN
A**LSVLTWPTHAAPWTCALNGLGGSLRSILHR
LMKRRDRDYLW*CQCLTGIPVASPSLRSLSLTTS
*QTCLMLGMPLHICQP*CNIWLTTTNTGRH*MT
*SAKV*GFHLTAKAKPQRGPLDRQRTL*ITVA*
TRGLPF**Q*QVLVKELMFNI*P*IIQVPKFHS*
KVMFHEEKYMFF*ILNDRTNTWQTPLLCCHPVY
PCQSMELVHCN*QATGSKALVPVSSSPRMVTK*
LS**SAHPLP*KSSLLFSLTVFLKNIDLKDS*GA
YLNRFI*EKKKTT*NK*NN*DQITDKLVSFTAS
MATWFFWPMV*HLS*NTALAGGCPL*TGISSLC
NPFPVKGVHLNKVIHDEGKSGISFLGNP*ASFIS
GSQRQPRGTTPSII*KPLNLNALGQAFLKKKKK

Frame 2

GRGRGRARGGARPRDGLRPQHPCLAERRDLLPG
LGRVQLAPPDHQRVAGPGGTPARPLRPDRRRRR
HPPEPRVGTPQRSPRPQGPHRAGQR*QRGFRSP
RRDHQQGPEAPLLQQRRGRDSDLLHQREAGVFCG
GAHRAHETDAGPYSGFADLCKGR*SERWLLVGL
RQSWL*MQYCSDSRVSP*MLS**AS*NYCN*S*
TNSELRCRSSVQVDPGHKSLRAHGDPRSGFASIG
*P*RGVSPSSSPRRLQQEIYGE*QHNCLL**TD
SNRTWGSSLPVQITGL*FSVYSIRSLS*SHRNN
KR*PRDSVCQPSLRKDDGLPQR*APGKRTR*SAQ
KR*EPGRPSRHHQYMHQEGKGVAGGLLCQTEIR
GQHPTAREDHPSDWPRREN*AFCLAQETVLYH*
Q**ADSQDSS*FRRQFSDRASFIQI*EQEERVH*
REIDIISRQ*CTKPAESSLSVHGEDPLHDHRGS
HHKGRLEKTVRKRVCVY*ECAPESQSPCNANNH
Q*CSPLYLSIT***GELGLQHL*IGSHYA*KAIG
LSGLKGLLSVWSM*IFKLF*NHSSGLVPSDRSQ
LPLFQCLPQLHPCCRRPARHRFLSWKGKSKGKP
RSVG*GGSPHCCHSP*RGSPGKDQLFPLQCRQ*A
CCALQ*HCCSGESPHRPGLPAHGQGHQMQHFQE
Y*QEPLSNAAPGYY*HGFGNRDDKTL*TCE*VC
EQHQQANGS*D*RQRL*MQPCWEELP*KPNPDQT
HDD*VC*RGQPMPPLGPVH*MGWEDL*GVFCTD
**REETGTTCGDASV*PEYL*HPQVSDLFH*LL
HNRHV*CLGCLCTSASPDATFG*QLQTLEDTR*P
KVQKFEASI*QLKPSHRGGLLTDKGHCESQ*RK
REAFLSNDNDRYW*RS*CLIFDLESFKSPNFIL
RKLCSMKKNICSFEYLMTEQILGKLLCSAVILCT
LVNPWSWFTVTSRPQEAKPWCL*AHLPGW*LSS
LASDQLILYHKSHHCCLA*LFSSRTSI*RIHKE
LI*TDLSKKKKKRHKISETTRTKLQIN*LASQPL
WLHGSSGRWYDT*VRTQPWLVGALSRLVSAACV
TPFL*KGFILTKSSMMREKVAFHFWGIHELPLF
LAHRGSHEALHQVLYKSH*I*MPLDKLFLKKKKK

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