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Sequence NM_145182
GAGGGCGCGATCCTGGCGTCCCCCGACGGCCTGGGGCCCCAATCCAGAGGCCTGGGTGGGAGGGGACCAAGGGTGTAGTAAGGAAGCGCCTTTTGCTGGAGGGCAACGGACCGGGGCGGGGAGTCGGGAGACCAGAGTGGGAGGAAGGCGGGGAGTCCAGGTTCCGCCCCGGAGCCGACTTCCTCCTGGTCGGCGGCTGC
AGCGGGGTGAGCGGCGGCAGCGGCCGGGGATCCTGGAGCCATGGGGCGCGCGCGCGACGCCATCCTGGATGCGCTGGAGAACCTGACCGCCGAGGAGCTC
AAGAAGTTCAAGCTGAAGCTGCTGTCGGTGCCGCTGCGCGAGGGCTACGGGCGCATCCCGCGGGGCGCGCTGCTGTCCATGGACGCCTTGGACCTCACCG
ACAAGCTGGTCAGCTTCTACCTGGAGACCTACGGCGCCGAGCTCACCGCTAACGTGCTGCGCGACATGGGCCTGCAGGAGATGGCCGGGCAGCTGCAGGC
GGCCACGCACCAGGGCCTGCACTTTATAGACCAGCACCGGGCTGCGCTTATCGCGAGGGTCACAAACGTTGAGTGGCTGCTGGATGCTCTGTACGGGAAG
GTCCTGACGGATGAGCAGTACCAGGCAGTGCGGGCCGAGCCCACCAACCCAAGCAAGATGCGGAAGCTCTTCAGTTTCACACCAGCCTGGAACTGGACCT
GCAAGGACTTGCTCCTCCAGGCCCTAAGGGAGTCCCAGTCCTACCTGGTGGAGGACCTGGAGCGGAGCTGAGGCTCCTTCCCAGCAACACTCCGGTCAGC
CCCTGGCAATCCCACCAAATCATCCTGAATCTGATCTTTTTATACACAATATACGAAAAGCCAGCTTGAA
- refseq_PYCARD.F1 (forward primer)
- refseq_PYCARD.R1 (reverse primer)
- Single exon skipping
Frame 0
EGAILASPDGLGPQSRGLGGRGPRV**GSAFCWRATDRGGESGDQSGRKAGSPGSAPEPTSSWSAAA
AG*AAAAAGDPGAMGRARDAILDALENLTAEEL
KKFKLKLLSVPLREGYGRIPRGALLSMDALDLT
DKLVSFYLETYGAELTANVLRDMGLQEMAGQLQA
ATHQGLHFIDQHRAALIARVTNVEWLLDALYGK
VLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWT
CKDLLLQALRESQSYLVEDLERS*GSFPATLRSA
PGNPTKSS*I*SFYTQYTKSQLE
Frame 1
RARSWRPPTAWGPNPEAWVGGDQGCSKEAPFAGGQRTGAGSRETRVGGRRGVQVPPRSRLPPGRRL
QRGERRQRPGILEPWGARATPSWMRWRT*PPRSS
RSSS*SCCRCRCARATGASRGARCCPWTPWTSP
TSWSASTWRPTAPSSPLTCCATWACRRWPGSCR
RPRTRACTL*TSTGLRLSRGSQTLSGCWMLCTGR
S*RMSSTRQCGPSPPTQARCGSSSVSHQPGTGP
ARTCSSRP*GSPSPTWWRTWSGAEAPSQQHSGQ
PLAIPPNHPESDLFIHNIRKASL
Frame 2
GRDPGVPRRPGAPIQRPGWEGTKGVVRKRLLLEGNGPGRGVGRPEWEEGGESRFRPGADFLLVGGC
SGVSGGSGRGSWSHGARARRHPGCAGEPDRRGA
QEVQAEAAVGAAARGLRAHPAGRAAVHGRLGPHR
QAGQLLPGDLRRRAHR*RAARHGPAGDGRAAAG
GHAPGPALYRPAPGCAYREGHKR*VAAGCSVRE
GPDG*AVPGSAGRAHQPKQDAEALQFHTSLELDL
QGLAPPGPKGVPVLPGGGPGAELRLLPSNTPVS
PWQSHQIILNLIFLYTIYEKPA*
Sequence NM_013258
GAGGGCGCGATCCTGGCGTCCCCCGACGGCCTGGGGCCCCAATCCAGAGGCCTGGGTGGGAGGGGACCAAGGGTGTAGTAAGGAAGCGCCTTTTGCTGGAGGGCAACGGACCGGGGCGGGGAGTCGGGAGACCAGAGTGGGAGGAAGGCGGGGAGTCCAGGTTCCGCCCCGGAGCCGACTTCCTCCTGGTCGGCGGCTGC
AGCGGGGTGAGCGGCGGCAGCGGCCGGGGATCCTGGAGCCATGGGGCGCGCGCGCGACGCCATCCTGGATGCGCTGGAGAACCTGACCGCCGAGGAGCTC
AAGAAGTTCAAGCTGAAGCTGCTGTCGGTGCCGCTGCGCGAGGGCTACGGGCGCATCCCGCGGGGCGCGCTGCTGTCCATGGACGCCTTGGACCTCACCG
ACAAGCTGGTCAGCTTCTACCTGGAGACCTACGGCGCCGAGCTCACCGCTAACGTGCTGCGCGACATGGGCCTGCAGGAGATGGCCGGGCAGCTGCAGGC
GGCCACGCACCAGGGCTCTGGAGCCGCGCCAGCTGGGATCCAGGCCCCTCCTCAGTCGGCAGCCAAGCCAGGCCTGCACTTTATAGACCAGCACCGGGCT
GCGCTTATCGCGAGGGTCACAAACGTTGAGTGGCTGCTGGATGCTCTGTACGGGAAGGTCCTGACGGATGAGCAGTACCAGGCAGTGCGGGCCGAGCCCA
CCAACCCAAGCAAGATGCGGAAGCTCTTCAGTTTCACACCAGCCTGGAACTGGACCTGCAAGGACTTGCTCCTCCAGGCCCTAAGGGAGTCCCAGTCCTA
CCTGGTGGAGGACCTGGAGCGGAGCTGAGGCTCCTTCCCAGCAACACTCCGGTCAGCCCCTGGCAATCCCACCAAATCATCCTGAATCTGATCTTTTTAT
ACACAATATACGAAAAGCCAGCTTGAA
- refseq_PYCARD.F1 (forward primer)
- refseq_PYCARD.R1 (reverse primer)
- Single exon skipping
Frame 0
EGAILASPDGLGPQSRGLGGRGPRV**GSAFCWRATDRGGESGDQSGRKAGSPGSAPEPTSSWSAAA
AG*AAAAAGDPGAMGRARDAILDALENLTAEEL
KKFKLKLLSVPLREGYGRIPRGALLSMDALDLT
DKLVSFYLETYGAELTANVLRDMGLQEMAGQLQA
ATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRA
ALIARVTNVEWLLDALYGKVLTDEQYQAVRAEP
TNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSY
LVEDLERS*GSFPATLRSAPGNPTKSS*I*SFY
TQYTKSQLE
Frame 1
RARSWRPPTAWGPNPEAWVGGDQGCSKEAPFAGGQRTGAGSRETRVGGRRGVQVPPRSRLPPGRRL
QRGERRQRPGILEPWGARATPSWMRWRT*PPRSS
RSSS*SCCRCRCARATGASRGARCCPWTPWTSP
TSWSASTWRPTAPSSPLTCCATWACRRWPGSCR
RPRTRALEPRQLGSRPLLSRQPSQACTL*TSTGL
RLSRGSQTLSGCWMLCTGRS*RMSSTRQCGPSP
PTQARCGSSSVSHQPGTGPARTCSSRP*GSPSP
TWWRTWSGAEAPSQQHSGQPLAIPPNHPESDLFI
HNIRKASL
Frame 2
GRDPGVPRRPGAPIQRPGWEGTKGVVRKRLLLEGNGPGRGVGRPEWEEGGESRFRPGADFLLVGGC
SGVSGGSGRGSWSHGARARRHPGCAGEPDRRGA
QEVQAEAAVGAAARGLRAHPAGRAAVHGRLGPHR
QAGQLLPGDLRRRAHR*RAARHGPAGDGRAAAG
GHAPGLWSRASWDPGPSSVGSQARPALYRPAPG
CAYREGHKR*VAAGCSVREGPDG*AVPGSAGRAH
QPKQDAEALQFHTSLELDLQGLAPPGPKGVPVL
PGGGPGAELRLLPSNTPVSPWQSHQIILNLIFL
YTIYEKPA*
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