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Sequence HSC20.dAug05
GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTGTAAGCTAAACA
GAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAA
GAAAAGATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCC
AGCACTTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAAT
TAGCCGGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAG
ATCACGCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.10 (forward primer)
- HSC20.au.2.r (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSGCGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTL*AKQ
KEFTDNVSSAFEQDDFEEAKEILTKMRYFSNIE
EKIKLKKIPL*LWIV*SLKNKVLAGHSGSHL*S
QHFGRLRWVDDKVRSSRPAWPT**NPVSAENTKI
SRAWWRVPVIPATW*AEAGESLKPVRWRLQ*AE
ITQLHSSLGNRVRLNLEK*INEK*NSCIF
Frame 1
SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPGVGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPCKLN
RKNLLTM*AVLLNKMTLKKPRKF*QR*DTFQI*K
KRSS*RRFPFNCG*FKV*KIKFLLGTVAHTCNP
STLGG*GGWMTRSGVQDQLGQHSETPSLLKIQK
LAGHGGACL*SQLLGRPRQENRLNP*GGGCSEQR
SRNCTPAWATE*DLILKNK*MKNKILVYF
Frame 2
LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLVS*T
ERIY*QCEQCF*TR*L*RSQGNFDKDEILFKYR
RKDQVKEDSPLIVDSLKFKK*SSCWAQWLTPVIP
ALWEAEVGG*QGQEFKTSLANIVKPRLC*KYKN
*PGMVARACNPSYLVGRGRRIA*TREVEVAVSR
DHATALQLGQQSET*S*KINK*KIKFLYI
Sequence HSC20.eAug05
GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGACTGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACC
CTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATTCCTCATAG
AAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATCCATTGTCAAAGCTAAACAGAAAGAATTTACTGA
CAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAGTTA
AAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGC
TGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGGGCATGGTG
GCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCACGCAACTGCAC
TCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.10 (forward primer)
- HSC20.au.2.r (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSGCGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTD*KGLLREAFDPGE*CL*D
PPGPPEQRTVPSKAPWNRDS*KDRL*NGQAIPHR
NNGNQ*KTRRS*K*SCHERD*IHCQS*TERIY*
QCEQCF*TR*L*RSQGNFDKDEILFKYRRKDQV
KEDSPLIVDSLKFKK*SSCWAQWLTPVIPALWEA
EVGG*QGQEFKTSLANIVKPRLC*KYKN*PGMV
ARACNPSYLVGRGRRIA*TREVEVAVSRDHATA
LQLGQQSET*S*KINK*KIKFLYI
Frame 1
SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPGVGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQTEKDFSEKHSTLVNDAYKT
LLAPLSRGLYLLKLHGIEIPERTDYEMDRQFLI
EIMEINEKLAEAESEAAMKEIESIVKAKQKEFTD
NVSSAFEQDDFEEAKEILTKMRYFSNIEEKIKL
KKIPL*LWIV*SLKNKVLAGHSGSHL*SQHFGR
LRWVDDKVRSSRPAWPT**NPVSAENTKISRAWW
RVPVIPATW*AEAGESLKPVRWRLQ*AEITQLH
SSLGNRVRLNLEK*INEK*NSCIF
Frame 2
LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRLKRTSQRSIRPW*MMPIRP
SWPP*AEDCTF*SSME*RFLKGQIMKWTGNSS*
K*WKSMKNSQKLKVKLP*KRLNPLSKLNRKNLL
TM*AVLLNKMTLKKPRKF*QR*DTFQI*KKRSS*
RRFPFNCG*FKV*KIKFLLGTVAHTCNPSTLGG
*GGWMTRSGVQDQLGQHSETPSLLKIQKLAGHG
GACL*SQLLGRPRQENRLNP*GGGCSEQRSRNCT
PAWATE*DLILKNK*MKNKILVYF
Sequence HSC20.cAug05
GGTACTTGAGGGGCAGGGCCTGGGAGACTGGAAGACTTGAATGAATAGGCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAG
ACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATTCCTCA
TAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATCCATTGTCAAAGCTAAACAGAAAGAATTTAC
TGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAG
TTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGA
GGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGGGCATG
GTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCACGCAACTG
CACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.10 (forward primer)
- HSC20.au.2.r (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
GT*GAGPGRLEDLNE*ATVPSELIQRSSSTGTSNCSVLSTQISSARGLRLKRTSQRSIRPW*MMPIR
PSWPP*AEDCTF*SSME*RFLKGQIMKWTGNSS
*K*WKSMKNSQKLKVKLP*KRLNPLSKLNRKNL
LTM*AVLLNKMTLKKPRKF*QR*DTFQI*KKRSS
*RRFPFNCG*FKV*KIKFLLGTVAHTCNPSTLG
G*GGWMTRSGVQDQLGQHSETPSLLKIQKLAGH
GGACL*SQLLGRPRQENRLNP*GGGCSEQRSRNC
TPAWATE*DLILKNK*MKNKILVYF
Frame 1
VLEGQGLGDWKT*MNRQPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD*KGLLREAFDPGE*CL*
DPPGPPEQRTVPSKAPWNRDS*KDRL*NGQAIPH
RNNGNQ*KTRRS*K*SCHERD*IHCQS*TERIY
*QCEQCF*TR*L*RSQGNFDKDEILFKYRRKDQ
VKEDSPLIVDSLKFKK*SSCWAQWLTPVIPALWE
AEVGG*QGQEFKTSLANIVKPRLC*KYKN*PGM
VARACNPSYLVGRGRRIA*TREVEVAVSRDHAT
ALQLGQQSET*S*KINK*KIKFLYI
Frame 2
YLRGRAWETGRLE*IGNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYK
TLLAPLSRGLYLLKLHGIEIPERTDYEMDRQFL
IEIMEINEKLAEAESEAAMKEIESIVKAKQKEFT
DNVSSAFEQDDFEEAKEILTKMRYFSNIEEKIK
LKKIPL*LWIV*SLKNKVLAGHSGSHL*SQHFG
RLRWVDDKVRSSRPAWPT**NPVSAENTKISRAW
WRVPVIPATW*AEAGESLKPVRWRLQ*AEITQL
HSSLGNRVRLNLEK*INEK*NSCIF
Sequence HSC20.aAug05
GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTCTAAAGCTCCAT
GGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATTCCTCATAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAG
CTGCCATGAAAGAGATTGAATCCATTGTCAAAGCTAAACAGAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAA
GGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAAT
AAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGC
CAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGA
ATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCACGCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATA
AATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.10 (forward primer)
- HSC20.au.2.r (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSGCGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTF*SSM
E*RFLKGQIMKWTGNSS*K*WKSMKNSQKLKVK
LP*KRLNPLSKLNRKNLLTM*AVLLNKMTLKKP
RKF*QR*DTFQI*KKRSS*RRFPFNCG*FKV*KI
KFLLGTVAHTCNPSTLGG*GGWMTRSGVQDQLG
QHSETPSLLKIQKLAGHGGACL*SQLLGRPRQE
NRLNP*GGGCSEQRSRNCTPAWATE*DLILKNK*
MKNKILVYF
Frame 1
SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPGVGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPSKAP
WNRDS*KDRL*NGQAIPHRNNGNQ*KTRRS*K*S
CHERD*IHCQS*TERIY*QCEQCF*TR*L*RSQ
GNFDKDEILFKYRRKDQVKEDSPLIVDSLKFKK
*SSCWAQWLTPVIPALWEAEVGG*QGQEFKTSLA
NIVKPRLC*KYKN*PGMVARACNPSYLVGRGRR
IA*TREVEVAVSRDHATALQLGQQSET*S*KIN
K*KIKFLYI
Frame 2
LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLLKLH
GIEIPERTDYEMDRQFLIEIMEINEKLAEAESE
AAMKEIESIVKAKQKEFTDNVSSAFEQDDFEEAK
EILTKMRYFSNIEEKIKLKKIPL*LWIV*SLKN
KVLAGHSGSHL*SQHFGRLRWVDDKVRSSRPAW
PT**NPVSAENTKISRAWWRVPVIPATW*AEAGE
SLKPVRWRLQ*AEITQLHSSLGNRVRLNLEK*I
NEK*NSCIF
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