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Sequences

Sequence HSC20.dAug05

GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGT
GTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTGTAAGCTAAACA
GAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAA
GAAAAGATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCC
AGCACTTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAAT
TAGCCGGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAG
ATCACGCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
  • HSC20.u.f.10 (forward primer)
  • HSC20.au.2.r (reverse primer)
  • Single exon skipping

Frame 0

VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSG
CGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTL*AKQ
KEFTDNVSSAFEQDDFEEAKEILTKMRYFSNIE
EKIKLKKIPL*LWIV*SLKNKVLAGHSGSHL*S
QHFGRLRWVDDKVRSSRPAWPT**NPVSAENTKI
SRAWWRVPVIPATW*AEAGESLKPVRWRLQ*AE
ITQLHSSLGNRVRLNLEK*INEK*NSCIF

Frame 1

SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPG
VGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPCKLN
RKNLLTM*AVLLNKMTLKKPRKF*QR*DTFQI*K
KRSS*RRFPFNCG*FKV*KIKFLLGTVAHTCNP
STLGG*GGWMTRSGVQDQLGQHSETPSLLKIQK
LAGHGGACL*SQLLGRPRQENRLNP*GGGCSEQR
SRNCTPAWATE*DLILKNK*MKNKILVYF

Frame 2

LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRV
WGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLVS*T
ERIY*QCEQCF*TR*L*RSQGNFDKDEILFKYR
RKDQVKEDSPLIVDSLKFKK*SSCWAQWLTPVIP
ALWEAEVGG*QGQEFKTSLANIVKPRLC*KYKN
*PGMVARACNPSYLVGRGRRIA*TREVEVAVSR
DHATALQLGQQSET*S*KINK*KIKFLYI

Sequence HSC20.gAug05

GGTACTTGAGGGGCAGGGCCTGGGAGACTGGAAGACTTGAATGAATAGGCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCA
ACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAG
ACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTGTAAGCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATT
CCTCATAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATCCATTGTCAAAG
CTAAACAGAAAGAA
TTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGA
TCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTT
TGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGG
GCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCACGC
AACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
  • HSC20.u.f.10 (forward primer)
  • HSC20.au.2.r (reverse primer)
  • Single exon skipping

Frame 0

GT*GAGPGRLEDLNE*ATVPSELIQRSSSTGTS
NCSVLSTQISSARGLRLKRTSQRSIRPW*MMPIR
PSWPP*AEDCTL*AKAPWNRDS*KDRL*NGQAI
PHRNNGNQ*KTRRS*K*SCHERD*IHCQS
*TER
IY*QCEQCF*TR*L*RSQGNFDKDEILFKYRRKD
QVKEDSPLIVDSLKFKK*SSCWAQWLTPVIPAL
WEAEVGG*QGQEFKTSLANIVKPRLC*KYKN*P
GMVARACNPSYLVGRGRRIA*TREVEVAVSRDHA
TALQLGQQSET*S*KINK*KIKFLYI

Frame 1

VLEGQGLGDWKT*MNRQPFLQS*YSEAPAQVPA
TAASCPPRFLQPEVSD*KGLLREAFDPGE*CL*
DPPGPPEQRTVPCKLKLHGIEIPERTDYEMDRQF
LIEIMEINEKLAEAESEAAMKEIESIVK
AKQKE
FTDNVSSAFEQDDFEEAKEILTKMRYFSNIEEK
IKLKKIPL*LWIV*SLKNKVLAGHSGSHL*SQHF
GRLRWVDDKVRSSRPAWPT**NPVSAENTKISR
AWWRVPVIPATW*AEAGESLKPVRWRLQ*AEIT
QLHSSLGNRVRLNLEK*INEK*NSCIF

Frame 2

YLRGRAWETGRLE*IGNRSFRVDTAKLQHRYQQ
LQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYK
TLLAPLSRGLYLVS*SSME*RFLKGQIMKWTGN
SS*K*WKSMKNSQKLKVKLP*KRLNPLSK
LNRKN
LLTM*AVLLNKMTLKKPRKF*QR*DTFQI*KKR
SS*RRFPFNCG*FKV*KIKFLLGTVAHTCNPST
LGG*GGWMTRSGVQDQLGQHSETPSLLKIQKLAG
HGGACL*SQLLGRPRQENRLNP*GGGCSEQRSR
NCTPAWATE*DLILKNK*MKNKILVYF

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