Sequences - Rnomics 📊 Palace

        ☰
    
        Rnomics 📊 Palace
    
                       Back to Rnomics Platform
            
                       Open Session

1 warning:

Access restricted to public data.
Please sign in at Rnomics Platform to access your data.

Navigation

Home

Sequences

Sequence HIF1A.gAug05

TGGTATTATTCAGCACGACTTGATTTTCTCCCTTCAACAAACAGAATGTGTCCTTAAACCGGTTGAATCTTCAGATATGAAAATGACTCAGCTATTCACC AAAGTTGAATCAGAAGATACAAGTAGCCTCTTTGACAAACTTAAGAAGGAACCTGATGCTTTAACTTTGCTGGCCCCAGCCGCTGGAGACACAATCATAT CTTTAGATTTTGGCAGCAACGCCTAATAGTCCCAGTGAATATTGTTTTTATGTGGATAGTGATATGGTCAATGAATTCAAGTTGGAATTGGTAGAAAAAC TTTTTGCTGAAGACACAGAAGCAAAGAACCCATTTTCTACTCAGGACACAGATTTAGACTTGGAGATGTTAGCTCCCTATATCCCAATGGATGATGACTT CCAGTTACGTTCCTTCGATCAGTTGTCACCATTAGAAAGCAGTTCCGCAAGCCCTGAAAGCGCAAGTCCTCAAAGCACAGTTACAGTATTCCAGCAGACT CAAATACAAGAACCTACTGCTAATGCCACCACTACCACTGCCACCACTGATGAATTAAAAACAGTGACAAAAGACCGTATGGAAGACATTAAAATATTGA TTGCATCTCCATCTCCTACCCACATACATAAAGAAA

HIF1A.au.2.f (forward primer)
HIF1A.u.r.13 (reverse primer)
Single exon skipping

Frame 0

WYYSARLDFLPSTNRMCP*TG*IFRYENDSAIH QS*IRRYK*PL*QT*EGT*CFNFAGPSRWRHNHI FRFWQQRLIVPVNIVFMWIVIWSMNSSWNW*KN FLLKTQKQRTHFLLRTQI*TWRC*LPISQWMMT SSYVPSISCHH*KAVPQALKAQVLKAQLQYSSRL KYKNLLLMPPLPLPPLMN*KQ*QKTVWKTLKY* LHLHLLPTYIKK

Frame 1

GIIQHDLIFSLQQTECVLKPVESSDMKMTQLFT KVESEDTSSLFDKLKKEPDALTLLAPAAGDTII SLDFGSNA**SQ*ILFLCG**YGQ*IQVGIGRKT FC*RHRSKEPIFYSGHRFRLGDVSSLYPNG**L PVTFLRSVVTIRKQFRKP*KRKSSKHSYSIPAD SNTRTYC*CHHYHCHH**IKNSDKRPYGRH*NID CISISYPHT*R

Frame 2

VLFSTT*FSPFNKQNVSLNRLNLQI*K*LSYSP KLNQKIQVASLTNLRRNLML*LCWPQPLETQSY L*ILAATPNSPSEYCFYVDSDMVNEFKLELVEK LFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDF QLRSFDQLSPLESSSASPESASPQSTVTVFQQT QIQEPTANATTTTATTDELKTVTKDRMEDIKIL IASPSPTHIHKE

Sequence HIF1A.bAug05

CCCCTGACGCTGCCTCAGCTCCTCAGTGCACAGTGCTGCCTCGTCTGAGGGGACAGGAGGATCACCCTCTTCGTCGCTTCGGCCAGTGTGTCGGGCTGGG CCCTGACAAGCCACCTGAGGAGAGGCTCGGAGCCGGGCCCGGACCCCGGCGATTGCCGCCCGCTTCTCTCTAGTCTCACGAGGGGTTTCCCGCCTCGCAC CCCCACCTCTGGACTTGCCTTTCCTTCTCTTCTCCGCGTGTGGAGGGAGCCAGCGCTTAGGCCGGAGCGAGCCTGGGGGCCGCCCGCCGTGAAGACATCG CGGGGACCGATTCACCATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAAAGATAAGTTCTGAACGTCGAAAAGAAAAGTCTCGAGATGCAGCCAGATCT CGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCCACATAATGTGAGTTCGCATCTTGATAAGGCCTCTGTGATGAGGC TTACCATCAGCTATTTGCGTGTGAGGAAACTTCTGGATGCTGGTGATTTGGATATTGAAGATGACATGAAAGCACAGATGAATTGCTTTTATTTGAAAGC CTTGGATGGTTTTGTTATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAATGTGAACAAATACATGGGATTAACTCAGTTTGAACTAACT GGACACAGTGTGTTTGATTTTACTCATCCATGTGACCATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCTTGTGAAAAAGGGTAAAGAACAAA ACACACAGCGAAGCTTTTTTCTCAGAATGAAGTGTACCCTAACTAGCCGAGGAAGAACTATGAACATAAAGTCTGCAACATGGAAGGTATTGCACTGCAC AGGCCACATTCACGTATATGATACCAACAGTAACCAACCTCAGTGTGGGTATAAGAAACCACCTATGACCTGCTTGGTGCTGATTTGTGAACCCATTCCT CACCCATCAAATATTGAAATTCCTTTAGATAGCAAGACTTTCCTCAGTCGACACAGCCTGGATATGAAATTTTCTTATTGTGATGAAAGAATTACCGAAT TGATGGGATATGAGCCAGAAGAACTTTTAGGCCGCTCAATTTATGAATATTATCATGCTTTGGACTCTGATCATCTGACCAAAACTCATCATGATATGTT TACTAAAGGACAAGTCACCACAGGACAGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGGGTTGAAACTCAAGCAACTGTCATATATAACACCAAG AATTCTCAACCACAGTGCATTGTATGTGTGAATTACGTTGTGAGTGGTATTATTCAGCACGACTTGATTTTCTCCCTTCAACAAACAGAATGTGTCCTTA AACCGGTTGAATCTTCAGATATGAAAATGACTCAGCTATTCACCAAAGTTGAATCAGAAGATACAAGTAGCCTCTTTGACAAACTTAAGAAGGAACCTGA TGCTTTAACTTTGCTGGCCCCAGCCGCTGGAGACACAATCATATCTTTAGATTTTGGCAGCAACGACACAGAAACTGATGACCAGCAACTTGAGGAAGTA CCATTATATAATGATGTAATGCTCCCCTCACCCAACGAAAAATTACAGAATATAAATTTGGCAATGTCTCCATTACCCACCGCTGAAACGCCAAAGCCAC TTCGAAGTAGTGCTGACCCTGCACTCAATCAAGAAGTTGCATTAAAATTAGAACCAAATCCAGAGTCACTGGAACTTTCTTTTACCATGCCCCAGATTCA GGATCAGACACCTAGTCCTTCCGATGGAAGCACTAGACAAAGTTCACCTGAGCCTAATAGTCCCAGTGAATATTGTTTTTATGTGGATAGTGATATGGTC AATGAATTCAAGTTGGAATTGGTAGAAAAACTTTTTGCTGAAGACACAGAAGCAAAGAACCCATTTTCTACTCAGGACACAGATTTAGACTTGGAGATGT TAGCTCCCTATATCCCAATGGATGATGACTTCCAGTTACGTTCCTTCGATCAGTTGTCACCATTAGAAAGCAGTTCCGCAAGCCCTGAAAGCGCAAGTCC TCAAAGCACAGTTACAGTATTCCAGCAGACTCAAATACAAGAACCTACTGCTAATGCCACCACTACCACTGCCACCACTGATGAATTAAAAACAGTGACA AAAGACCGTATGGAAGACATTAAAATATTGATTGCATCTCCATCTCCTACCCACATACATAAAGAAACTACTAGTGCCACATCATCACCATATAGAGATA CTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAAAGGAGTCATAGAACAGACAGAAAAATCTCATCCAAGAAGCCCTAACGTGTTATCTGTCGCTTT GAGTCAAAGAACTACAGTTCCTGAGGAAGAACTAAATCCAAAGATACTAGCTTTGCAGAATGCTCAGAGAAAGCGAAAAATGGAACATGATGGTTCACTT TTTCAAGCAGTAGGAATTATTTAGCATGTAGACTGCTGGGGCAATCAATGGATGAAAGTGGATTACCACAGCTGACCAGTTATGATTGTGAAGTTAATGC TCCTATACAAGGCAGCAGAAACCTACTGCAGGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGAGCTTTTTCTTAATTTCATTCCTTTTTTTGG ACACTGGTGGCTCATTACCTAAAGCAGTCTATTTATATTTTCTACATCTAATTTTAGAAGCCTGGCTACAATACTGCACAAACTTGGTTAGTTCAATTTT GATCCCCTTTCTACTTAATTTACATTAATGCTCTTTTTTAGTATGTTCTTTAATGCTGGATCACAGACAGCTCATTTTCTCAGTTTTTTGGTATTTAAAC CATTGCATTGCAGTAGCATCATTTTAAAAAATGCACCTTTTTATTTATTTATTTTTGGCTAGGGAGTTTATCCCTTTTTCGAATTATTTTTAAGAAGATG CCAATATAATTTTTGTAAGAAGGCAGTAACCTTTCATCATGATCATAGGCAGTTGAAAAATTTTTACACCTTTTTTTTCACATTTTACATAAATAATAAT GCTTTGCCAGCAGTACGTGGTAGCCACAATTGCACAATATATTTTCTTAAAAAATACCAGCAGTTACTCATGGAATATATTCTGCGTTTATAAAACTAGT TTTTAAGAAGAAATTTTTTTTGGCCTATGAAATTGTTAAACCTGGAACATGACATTGTTAATCATATAATAATGATTCTTAAATGCTGTATGGTTTATTA TTTAAATGGGTAAAGCCATTTACATAATATAGAAAGATATGCATATATCTAGAAGGTATGTGGCATTTATTTGGATAAAATTCTCAATTCAGAGAAATCA TCTGATGTTTCTATAGTCACTTTGCCAGCTCAAAAGAAAACAATACCCTATGTAGTTGTGGAAGTTTATGCTAATATTGTGTAACTGATATTAAACCTAA ATGTTCTGCCTACCCTGTTGGTATAAAGATATTTTGAGCAGACTGTAAACAAGAAAAAAAAAATCATGCATTCTTAGCAAAATTGCCTAGTATGTTAATT TGCTCAAAATACAATGTTTGATTTTATGCACTTTGTCGCTATTAACATCCTTTTTTTCATGTAGATTTCAATAATTGAGTAATTTTAGAAGCATTATTTT AGGAATATATAGTTGTCACAGTAAATATCTTGTTTTTTCTATGTACATTGTACAAATTTTTCATTCCTTTTGCTCTTTGTGGTTGGATCTAACACTAACT GTATTGTTTTGTTACATCAAATAAACATCTTCTGTGGACCAGGCCCCTTTGATCAGCTTTTATGTTCAAATATTAATAATATTTGCTTCAACACCTCCAA CTCATAAAATTGTTTACCAACAATTTAAGCACTTATGAAAATTACATGGTACTGGTTATTTCTACATTTATCTTAGTGCCATCACCTTAATGTATGTTGA GTCCCTAAATGTCATGTTAAATAATAACAACCATAATATCCCATTGAAAAGAGTATGTTGTTAGAAAAGAAACATCATTTTTAAGTTTCTGAGCCTATTA AAATGCTCAAACACAAAATATTAGTATTTTTAAAATATGAATGGGATGAGTGAAGCAGTTCTCAGCATTATAGTCACAATGTTACAAAGGCTAGAGCTTC TCTGAAGATTTCTAATCTGTTCCCATTAACAGATTAATAAATTTAGACTTCAAATGAATAATTTGCCCAAGCTTTAAAAGTAATAGATGGCAGACCAAAA ATGTAAGCTTAAGTTTCCTGACTCTAAAGTCAAACTTAGAACAAATTTGGTTTGTTTTTGTTTTAATGATACTGCGTTTTAAAACAAAGTAGCTTTATCC TTTTTCTCCTGTATTTTTCTTTTACAAAATAGCTGTATTTCTTTTATACTGATAATCTCATTTTTAAAAATCAGACAGTGTAGAAAGATATTTTTTAAAA CAGAAAAATCACTATGAATCCCTGCACCTACAGG

HIF1A.au.2.f (forward primer)
HIF1A.u.r.13 (reverse primer)
Single exon skipping

Frame 0

PLTLPQLLSAQCCLV*GDRRITLFVASASVSGW ALTSHLRRGSEPGPDPGDCRPLLSSLTRGFPPRT PTSGLAFPSLLRVWREPALRPERAWGPPAVKTS RGPIHHGGRRRRERQEKDKF*TSKRKVSRCSQI SAK*RI*SFL*ACSSVATST*CEFAS**GLCDEA YHQLFACEETSGCW*FGY*R*HESTDELLLFES LGWFCYGSHR*W*HDLHF**CEQIHGINSV*TN WTQCV*FYSSM*P*GNERNAYTQKWPCEKG*RTK HTAKLFSQNEVYPN*PRKNYEHKVCNMEGIALH RPHSRI*YQQ*PTSVWV*ETTYDLLGADL*THS SPIKY*NSFR*QDFPQSTQPGYEIFLL**KNYRI DGI*ARRTFRPLNL*ILSCFGL*SSDQNSS*YV Y*RTSHHRTVQDACQKRWICLG*NSSNCHI*HQ EFSTTVHCMCELRCEWYYSARLDFLPSTNRMCP* TG*IFRYENDSAIHQS*IRRYK*PL*QT*EGT* CFNFAGPSRWRHNHIFRFWQQRHRN**PAT*GS TII**CNAPLTQRKITEYKFGNVSITHR*NAKAT SK*C*PCTQSRSCIKIRTKSRVTGTFFYHAPDS GSDT*SFRWKH*TKFT*A**SQ*ILFLCG**YG Q*IQVGIGRKTFC*RHRSKEPIFYSGHRFRLGDV SSLYPNG**LPVTFLRSVVTIRKQFRKP*KRKS SKHSYSIPADSNTRTYC*CHHYHCHH**IKNSD KRPYGRH*NIDCISISYPHT*RNY*CHIITI*RY SKSDSLTKQSRKRSHRTDRKISSKKP*RVICRF ESKNYSS*GRTKSKDTSFAECSEKAKNGT*WFT FSSSRNYLACRLLGQSMDESGLPQLTSYDCEVNA PIQGSRNLLQGEELLRALDQVN*AFS*FHSFFW TLVAHYLKQSIYIFYI*F*KPGYNTAQTWLVQF *SPFYLIYINALF*YVL*CWITDSSFSQFFGI*T IALQ*HHFKKCTFLFIYFWLGSLSLFRIIFKKM PI*FL*EGSNLSS*S*AVEKFLHLFFHILHK** CFASSTW*PQLHNIFS*KIPAVTHGIYSAFIKLV FKKKFFLAYEIVKPGT*HC*SYNNDS*MLYGLL FKWVKPFT*YRKICIYLEGMWHLFG*NSQFREI I*CFYSHFASSKENNTLCSCGSLC*YCVTDIKPK CSAYPVGIKIF*ADCKQEKKNHAFLAKLPSMLI CSKYNV*FYALCRY*HPFFHVDFNN*VILEALF *EYIVVTVNILFFLCTLYKFFIPFALCGWI*H*L YCFVTSNKHLLWTRPL*SAFMFKY**YLLQHLQ LIKLFTNNLSTYENYMVLVISTFILVPSP*CML SP*MSC*IITTIISH*KEYVVRKETSFLSF*AY* NAQTQNISIFKI*MG*VKQFSAL*SQCYKG*SF SEDF*SVPINRLINLDFK*IICPSFKSNRWQTK NVSLSFLTLKSNLEQIWFVFVLMILRFKTK*LYP FSPVFFFYKIAVFLLY**SHF*KSDSVERYFLK QKNHYESLHLQ

Frame 1

P*RCLSSSVHSAASSEGTGGSPSSSLRPVCRAG P*QAT*GEARSRARTPAIAARFSLVSRGVSRLA PPPLDLPFLLFSACGGSQRLGRSEPGGRPP*RHR GDRFTMEGAGGANDKKKISSERRKEKSRDAARS RRSKESEVFYELAHQLPLPHNVSSHLDKASVMR LTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKA LDGFVMVLTDDGDMIYISDNVNKYMGLTQFELT GHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQ NTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCT GHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIP HPSNIEIPLDSKTFLSRHSLDMKFSYCDERITE LMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMF TKGQVTTGQYRMLAKRGGYVWVETQATVIYNTK NSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVL KPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPD ALTLLAPAAGDTIISLDFGSNDTETDDQQLEEV PLYNDVMLPSPNEKLQNINLAMSPLPTAETPKP LRSSADPALNQEVALKLEPNPESLELSFTMPQIQ DQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMV NEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEM LAPYIPMDDDFQLRSFDQLSPLESSSASPESASP QSTVTVFQQTQIQEPTANATTTTATTDELKTVT KDRMEDIKILIASPSPTHIHKETTSATSSPYRD TQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVAL SQRTTVPEEELNPKILALQNAQRKRKMEHDGSL FQAVGII*HVDCWGNQWMKVDYHS*PVMIVKLM LLYKAAETYCRVKNYSELWIKLTELFLNFIPFFG HWWLIT*SSLFIFSTSNFRSLATILHKLG*FNF DPLST*FTLMLFFSMFFNAGSQTAHFLSFLVFK PLHCSSIILKNAPFYLFIFG*GVYPFFELFLRRC QYNFCKKAVTFHHDHRQLKNFYTFFFTFYINNN ALPAVRGSHNCTIYFLKKYQQLLMEYILRL*N* FLRRNFFWPMKLLNLEHDIVNHIIMILKCCMVYY LNG*SHLHNIERYAYI*KVCGIYLDKILNSEKS SDVSIVTLPAQKKTIPYVVVEVYANIV*LILNL NVLPTLLV*RYFEQTVNKKKKIMHS*QNCLVC*F AQNTMFDFMHFVAINILFFM*ISIIE*F*KHYF RNI*LSQ*ISCFFYVHCTNFSFLLLFVVGSNTN CIVLLHQINIFCGPGPFDQLLCSNINNICFNTSN S*NCLPTI*ALMKITWYWLFLHLS*CHHLNVC* VPKCHVK**QP*YPIEKSMLLEKKHHF*VSEPI KMLKHKILVFLKYEWDE*SSSQHYSHNVTKARAS LKISNLFPLTD**I*TSNE*FAQALKVIDGRPK M*A*VS*L*SQT*NKFGLFLF**YCVLKQSSFI LFLLYFSFTK*LYFFYTDNLIFKNQTV*KDIF*N RKITMNPCTYR

Frame 2

PDAASAPQCTVLPRLRGQEDHPLRRFGQCVGLG PDKPPEERLGAGPGPRRLPPASL*SHEGFPASH PHLWTCLSFSSPRVEGASA*AGASLGAARREDI AGTDSPWRAPAARTTRKR*VLNVEKKSLEMQPDL GEVKNLKFFMSLLISCHFHIM*VRILIRPL**G LPSAICV*GNFWMLVIWILKMT*KHR*IAFI*K PWMVLLWFSQMMVT*FTFLIM*TNTWD*LSLN*L DTVCLILLIHVTMRK*EKCLHTEMAL*KRVKNK THSEAFFSE*SVP*LAEEEL*T*SLQHGRYCTA QATFTYMIPTVTNLSVGIRNHL*PAWC*FVNPFL THQILKFL*IARLSSVDTAWI*NFLIVMKELPN *WDMSQKNF*AAQFMNIIMLWTLII*PKLIMIC LLKDKSPQDSTGCLPKEVDMSGLKLKQLSYITPR ILNHSALYV*ITL*VVLFSTT*FSPFNKQNVSL NRLNLQI*K*LSYSPKLNQKIQVASLTNLRRNL ML*LCWPQPLETQSYL*ILAATTQKLMTSNLRKY HYIMM*CSPHPTKNYRI*IWQCLHYPPLKRQSH FEVVLTLHSIKKLH*N*NQIQSHWNFLLPCPRF RIRHLVLPMEALDKVHLSLIVPVNIVFMWIVIWS MNSSWNW*KNFLLKTQKQRTHFLLRTQI*TWRC *LPISQWMMTSSYVPSISCHH*KAVPQALKAQV LKAQLQYSSRLKYKNLLLMPPLPLPPLMN*KQ*Q KTVWKTLKY*LHLHLLPTYIKKLLVPHHHHIEI LKVGQPHQTEQEKES*NRQKNLIQEALTCYLSL *VKELQFLRKN*IQRY*LCRMLRESEKWNMMVHF FKQ*ELFSM*TAGAING*KWITTADQL*L*S*C SYTRQQKPTAG*RITQSFGSS*LSFFLISFLFL DTGGSLPKAVYLYFLHLILEAWLQYCTNLVSSIL IPFLLNLH*CSFLVCSLMLDHRQLIFSVFWYLN HCIAVASF*KMHLFIYLFLAREFIPFSNYF*ED ANIIFVRRQ*PFIMIIGS*KIFTPFFSHFT*IIM LCQQYVVATIAQYIFLKNTSSYSWNIFCVYKTS F*EEIFFGL*NC*TWNMTLLII***FLNAVWFI I*MGKAIYII*KDMHISRRYVAFIWIKFSIQRNH LMFL*SLCQLKRKQYPM*LWKFMLILCN*Y*T* MFCLPCWYKDILSRL*TRKKKSCILSKIA*YVN LLKIQCLILCTLSLLTSFFSCRFQ*LSNFRSIIL GIYSCHSKYLVFSMYIVQIFHSFCSLWLDLTLT VLFCYIK*TSSVDQAPLISFYVQILIIFASTPP THKIVYQQFKHL*KLHGTGYFYIYLSAITLMYVE SLNVMLNNNNHNIPLKRVCC*KRNIIFKFLSLL KCSNTKY*YF*NMNGMSEAVLSIIVTMLQRLEL L*RFLICSH*QINKFRLQMNNLPKL*K**MADQK CKLKFPDSKVKLRTNLVCFCFNDTAF*NKVALS FFSCIFLLQNSCISFILIISFLKIRQCRKIFFK TEKSL*IPAPT

Sequence HIF1A.aAug05

CCCCTGACGCTGCCTCAGCTCCTCAGTGCACAGTGCTGCCTCGTCTGAGGGGACAGGAGGATCACCCTCTTCGTCGCTTCGGCCAGTGTGTCGGGCTGGG CCCTGACAAGCCACCTGAGGAGAGGCTCGGAGCCGGGCCCGGACCCCGGCGATTGCCGCCCGCTTCTCTCTAGTCTCACGAGGGGTTTCCCGCCTCGCAC CCCCACCTCTGGACTTGCCTTTCCTTCTCTTCTCCGCGTGTGGAGGGAGCCAGCGCTTAGGCCGGAGCGAGCCTGGGGGCCGCCCGCCGTGAAGACATCG CGGGGACCGATTCACCATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAAAGATAAGTTCTGAACGTCGAAAAGAAAAGTCTCGAGATGCAGCCAGATCT CGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCCACATAATGTGAGTTCGCATCTTGATAAGGCCTCTGTGATGAGGC TTACCATCAGCTATTTGCGTGTGAGGAAACTTCTGGATGCTGGTGATTTGGATATTGAAGATGACATGAAAGCACAGATGAATTGCTTTTATTTGAAAGC CTTGGATGGTTTTGTTATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAATGTGAACAAATACATGGGATTAACTCAGTTTGAACTAACT GGACACAGTGTGTTTGATTTTACTCATCCATGTGACCATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCTTGTGAAAAAGGGTAAAGAACAAA ACACACAGCGAAGCTTTTTTCTCAGAATGAAGTGTACCCTAACTAGCCGAGGAAGAACTATGAACATAAAGTCTGCAACATGGAAGGTATTGCACTGCAC AGGCCACATTCACGTATATGATACCAACAGTAACCAACCTCAGTGTGGGTATAAGAAACCACCTATGACCTGCTTGGTGCTGATTTGTGAACCCATTCCT CACCCATCAAATATTGAAATTCCTTTAGATAGCAAGACTTTCCTCAGTCGACACAGCCTGGATATGAAATTTTCTTATTGTGATGAAAGAATTACCGAAT TGATGGGATATGAGCCAGAAGAACTTTTAGGCCGCTCAATTTATGAATATTATCATGCTTTGGACTCTGATCATCTGACCAAAACTCATCATGATATGTT TACTAAAGGACAAGTCACCACAGGACAGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGGGTTGAAACTCAAGCAACTGTCATATATAACACCAAG AATTCTCAACCACAGTGCATTGTATGTGTGAATTACGTTGTGAGTGGTATTATTCAGCACGACTTGATTTTCTCCCTTCAACAAACAGAATGTGTCCTTA AACCGGTTGAATCTTCAGATATGAAAATGACTCAGCTATTCACCAAAGTTGAATCAGAAGATACAAGTAGCCTCTTTGACAAACTTAAGAAGGAACCTGA TGCTTTAACTTTGCTGGCCCCAGCCGCTGGAGACACAATCATATCTTTAGATTTTGGCAGCAACGACACAGAAACTGATGACCAGCAACTTGAGGAAGTA CCATTATATAATGATGTAATGCTCCCCTCACCCAACGAAAAATTACAGAATATAAATTTGGCAATGTCTCCATTACCCACCGCTGAAACGCCAAAGCCAC TTCGAAGTAGTGCTGACCCTGCACTCAATCAAGAAGTTGCATTAAAATTAGAACCAAATCCAGAGTCACTGGAACTTTCTTTTACCATGCCCCAGATTCA GGATCAGACACCTAGTCCTTCCGATGGAAGCACTAGACAAAGTTCACCTGAGCCTAATAGTCCCAGTGAATATTGTTTTTATGTGGATAGTGATATGGTC AATGAATTCAAGTTGGAATTGGTAGAAAAACTTTTTGCTGAAGACACAGAAGCAAAGAACCCATTTTCTACTCAGGACACAGATTTAGACTTGGAGATGT TAGCTCCCTATATCCCAATGGATGATGACTTCCAGTTACGTTCCTTCGATCAGTTGTCACCATTAGAAAGCAGTTCCGCAAGCCCTGAAAGCGCAAGTCC TCAAAGCACAGTTACAGTATTCCAGCAGACTCAAATACAAGAACCTACTGCTAATGCCACCACTACCACTGCCACCACTGATGAATTAAAAACAGTGACA AAAGACCGTATGGAAGACATTAAAATATTGATTGCATCTCCATCTCCTACCCACATACATAAAGAAACTACTAGTGCCACATCATCACCATATAGAGATA CTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAAAGGAGTCATAGAACAGACAGAAAAATCTCATCCAAGAAGCCCTAACGTGTTATCTGTCGCTTT GAGTCAAAGAACTACAGTTCCTGAGGAAGAACTAAATCCAAAGATACTAGCTTTGCAGAATGCTCAGAGAAAGCGAAAAATGGAACATGATGGTTCACTT TTTCAAGCAGTAGGAATTGGAACATTATTACAGCAGCCAGACGATCATGCAGCTACTACATCACTTTCTTGGAAACGTGTAAAAGGATGCAAATCTAGTG AACAGAATGGAATGGAGCAAAAGACAATTATTTTAATACCCTCTGATTTAGCATGTAGACTGCTGGGGCAATCAATGGATGAAAGTGGATTACCACAGCT GACCAGTTATGATTGTGAAGTTAATGCTCCTATACAAGGCAGCAGAAACCTACTGCAGGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGAGCT TTTTCTTAATTTCATTCCTTTTTTTGGACACTGGTGGCTCATTACCTAAAGCAGTCTATTTATATTTTCTACATCTAATTTTAGAAGCCTGGCTACAATA CTGCACAAACTTGGTTAGTTCAATTTTGATCCCCTTTCTACTTAATTTACATTAATGCTCTTTTTTAGTATGTTCTTTAATGCTGGATCACAGACAGCTC ATTTTCTCAGTTTTTTGGTATTTAAACCATTGCATTGCAGTAGCATCATTTTAAAAAATGCACCTTTTTATTTATTTATTTTTGGCTAGGGAGTTTATCC CTTTTTCGAATTATTTTTAAGAAGATGCCAATATAATTTTTGTAAGAAGGCAGTAACCTTTCATCATGATCATAGGCAGTTGAAAAATTTTTACACCTTT TTTTTCACATTTTACATAAATAATAATGCTTTGCCAGCAGTACGTGGTAGCCACAATTGCACAATATATTTTCTTAAAAAATACCAGCAGTTACTCATGG AATATATTCTGCGTTTATAAAACTAGTTTTTAAGAAGAAATTTTTTTTGGCCTATGAAATTGTTAAACCTGGAACATGACATTGTTAATCATATAATAAT GATTCTTAAATGCTGTATGGTTTATTATTTAAATGGGTAAAGCCATTTACATAATATAGAAAGATATGCATATATCTAGAAGGTATGTGGCATTTATTTG GATAAAATTCTCAATTCAGAGAAATCATCTGATGTTTCTATAGTCACTTTGCCAGCTCAAAAGAAAACAATACCCTATGTAGTTGTGGAAGTTTATGCTA ATATTGTGTAACTGATATTAAACCTAAATGTTCTGCCTACCCTGTTGGTATAAAGATATTTTGAGCAGACTGTAAACAAGAAAAAAAAAATCATGCATTC TTAGCAAAATTGCCTAGTATGTTAATTTGCTCAAAATACAATGTTTGATTTTATGCACTTTGTCGCTATTAACATCCTTTTTTTCATGTAGATTTCAATA ATTGAGTAATTTTAGAAGCATTATTTTAGGAATATATAGTTGTCACAGTAAATATCTTGTTTTTTCTATGTACATTGTACAAATTTTTCATTCCTTTTGC TCTTTGTGGTTGGATCTAACACTAACTGTATTGTTTTGTTACATCAAATAAACATCTTCTGTGGACCAGGCCCCTTTGATCAGCTTTTATGTTCAAATAT TAATAATATTTGCTTCAACACCTCCAACTCATAAAATTGTTTACCAACAATTTAAGCACTTATGAAAATTACATGGTACTGGTTATTTCTACATTTATCT TAGTGCCATCACCTTAATGTATGTTGAGTCCCTAAATGTCATGTTAAATAATAACAACCATAATATCCCATTGAAAAGAGTATGTTGTTAGAAAAGAAAC ATCATTTTTAAGTTTCTGAGCCTATTAAAATGCTCAAACACAAAATATTAGTATTTTTAAAATATGAATGGGATGAGTGAAGCAGTTCTCAGCATTATAG TCACAATGTTACAAAGGCTAGAGCTTCTCTGAAGATTTCTAATCTGTTCCCATTAACAGATTAATAAATTTAGACTTCAAATGAATAATTTGCCCAAGCT TTAAAAGTAATAGATGGCAGACCAAAAATGTAAGCTTAAGTTTCCTGACTCTAAAGTCAAACTTAGAACAAATTTGGTTTGTTTTTGTTTTAATGATACT GCGTTTTAAAACAAAGTAGCTTTATCCTTTTTCTCCTGTATTTTTCTTTTACAAAATAGCTGTATTTCTTTTATACTGATAATCTCATTTTTAAAAATCA GACAGTGTAGAAAGATATTTTTTAAAACAGAAAAATCACTATGAATCCCTGCACCTACAGG

HIF1A.au.2.f (forward primer)
HIF1A.u.r.13 (reverse primer)
Single exon skipping

Frame 0

PLTLPQLLSAQCCLV*GDRRITLFVASASVSGW ALTSHLRRGSEPGPDPGDCRPLLSSLTRGFPPRT PTSGLAFPSLLRVWREPALRPERAWGPPAVKTS RGPIHHGGRRRRERQEKDKF*TSKRKVSRCSQI SAK*RI*SFL*ACSSVATST*CEFAS**GLCDEA YHQLFACEETSGCW*FGY*R*HESTDELLLFES LGWFCYGSHR*W*HDLHF**CEQIHGINSV*TN WTQCV*FYSSM*P*GNERNAYTQKWPCEKG*RTK HTAKLFSQNEVYPN*PRKNYEHKVCNMEGIALH RPHSRI*YQQ*PTSVWV*ETTYDLLGADL*THS SPIKY*NSFR*QDFPQSTQPGYEIFLL**KNYRI DGI*ARRTFRPLNL*ILSCFGL*SSDQNSS*YV Y*RTSHHRTVQDACQKRWICLG*NSSNCHI*HQ EFSTTVHCMCELRCEWYYSARLDFLPSTNRMCP* TG*IFRYENDSAIHQS*IRRYK*PL*QT*EGT* CFNFAGPSRWRHNHIFRFWQQRHRN**PAT*GS TII**CNAPLTQRKITEYKFGNVSITHR*NAKAT SK*C*PCTQSRSCIKIRTKSRVTGTFFYHAPDS GSDT*SFRWKH*TKFT*A**SQ*ILFLCG**YG Q*IQVGIGRKTFC*RHRSKEPIFYSGHRFRLGDV SSLYPNG**LPVTFLRSVVTIRKQFRKP*KRKS SKHSYSIPADSNTRTYC*CHHYHCHH**IKNSD KRPYGRH*NIDCISISYPHT*RNY*CHIITI*RY SKSDSLTKQSRKRSHRTDRKISSKKP*RVICRF ESKNYSS*GRTKSKDTSFAECSEKAKNGT*WFT FSSSRNWNIITAARRSCSYYITFLETCKRMQI** TEWNGAKDNYFNTL*FSM*TAGAING*KWITTA DQL*L*S*CSYTRQQKPTAG*RITQSFGSS*LS FFLISFLFLDTGGSLPKAVYLYFLHLILEAWLQY CTNLVSSILIPFLLNLH*CSFLVCSLMLDHRQL IFSVFWYLNHCIAVASF*KMHLFIYLFLAREFI PFSNYF*EDANIIFVRRQ*PFIMIIGS*KIFTPF FSHFT*IIMLCQQYVVATIAQYIFLKNTSSYSW NIFCVYKTSF*EEIFFGL*NC*TWNMTLLII** *FLNAVWFII*MGKAIYII*KDMHISRRYVAFIW IKFSIQRNHLMFL*SLCQLKRKQYPM*LWKFML ILCN*Y*T*MFCLPCWYKDILSRL*TRKKKSCI LSKIA*YVNLLKIQCLILCTLSLLTSFFSCRFQ* LSNFRSIILGIYSCHSKYLVFSMYIVQIFHSFC SLWLDLTLTVLFCYIK*TSSVDQAPLISFYVQI LIIFASTPPTHKIVYQQFKHL*KLHGTGYFYIYL SAITLMYVESLNVMLNNNNHNIPLKRVCC*KRN IIFKFLSLLKCSNTKY*YF*NMNGMSEAVLSII VTMLQRLELL*RFLICSH*QINKFRLQMNNLPKL *K**MADQKCKLKFPDSKVKLRTNLVCFCFNDT AF*NKVALSFFSCIFLLQNSCISFILIISFLKI RQCRKIFFKTEKSL*IPAPT

Frame 1

P*RCLSSSVHSAASSEGTGGSPSSSLRPVCRAG P*QAT*GEARSRARTPAIAARFSLVSRGVSRLA PPPLDLPFLLFSACGGSQRLGRSEPGGRPP*RHR GDRFTMEGAGGANDKKKISSERRKEKSRDAARS RRSKESEVFYELAHQLPLPHNVSSHLDKASVMR LTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKA LDGFVMVLTDDGDMIYISDNVNKYMGLTQFELT GHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQ NTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCT GHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIP HPSNIEIPLDSKTFLSRHSLDMKFSYCDERITE LMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMF TKGQVTTGQYRMLAKRGGYVWVETQATVIYNTK NSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVL KPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPD ALTLLAPAAGDTIISLDFGSNDTETDDQQLEEV PLYNDVMLPSPNEKLQNINLAMSPLPTAETPKP LRSSADPALNQEVALKLEPNPESLELSFTMPQIQ DQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMV NEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEM LAPYIPMDDDFQLRSFDQLSPLESSSASPESASP QSTVTVFQQTQIQEPTANATTTTATTDELKTVT KDRMEDIKILIASPSPTHIHKETTSATSSPYRD TQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVAL SQRTTVPEEELNPKILALQNAQRKRKMEHDGSL FQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSS EQNGMEQKTIILIPSDLACRLLGQSMDESGLPQL TSYDCEVNAPIQGSRNLLQGEELLRALDQVN*A FS*FHSFFWTLVAHYLKQSIYIFYI*F*KPGYN TAQTWLVQF*SPFYLIYINALF*YVL*CWITDSS FSQFFGI*TIALQ*HHFKKCTFLFIYFWLGSLS LFRIIFKKMPI*FL*EGSNLSS*S*AVEKFLHL FFHILHK**CFASSTW*PQLHNIFS*KIPAVTHG IYSAFIKLVFKKKFFLAYEIVKPGT*HC*SYNN DS*MLYGLLFKWVKPFT*YRKICIYLEGMWHLF G*NSQFREII*CFYSHFASSKENNTLCSCGSLC* YCVTDIKPKCSAYPVGIKIF*ADCKQEKKNHAF LAKLPSMLICSKYNV*FYALCRY*HPFFHVDFN N*VILEALF*EYIVVTVNILFFLCTLYKFFIPFA LCGWI*H*LYCFVTSNKHLLWTRPL*SAFMFKY **YLLQHLQLIKLFTNNLSTYENYMVLVISTFI LVPSP*CMLSP*MSC*IITTIISH*KEYVVRKET SFLSF*AY*NAQTQNISIFKI*MG*VKQFSAL* SQCYKG*SFSEDF*SVPINRLINLDFK*IICPS FKSNRWQTKNVSLSFLTLKSNLEQIWFVFVLMIL RFKTK*LYPFSPVFFFYKIAVFLLY**SHF*KS DSVERYFLKQKNHYESLHLQ

Frame 2

PDAASAPQCTVLPRLRGQEDHPLRRFGQCVGLG PDKPPEERLGAGPGPRRLPPASL*SHEGFPASH PHLWTCLSFSSPRVEGASA*AGASLGAARREDI AGTDSPWRAPAARTTRKR*VLNVEKKSLEMQPDL GEVKNLKFFMSLLISCHFHIM*VRILIRPL**G LPSAICV*GNFWMLVIWILKMT*KHR*IAFI*K PWMVLLWFSQMMVT*FTFLIM*TNTWD*LSLN*L DTVCLILLIHVTMRK*EKCLHTEMAL*KRVKNK THSEAFFSE*SVP*LAEEEL*T*SLQHGRYCTA QATFTYMIPTVTNLSVGIRNHL*PAWC*FVNPFL THQILKFL*IARLSSVDTAWI*NFLIVMKELPN *WDMSQKNF*AAQFMNIIMLWTLII*PKLIMIC LLKDKSPQDSTGCLPKEVDMSGLKLKQLSYITPR ILNHSALYV*ITL*VVLFSTT*FSPFNKQNVSL NRLNLQI*K*LSYSPKLNQKIQVASLTNLRRNL ML*LCWPQPLETQSYL*ILAATTQKLMTSNLRKY HYIMM*CSPHPTKNYRI*IWQCLHYPPLKRQSH FEVVLTLHSIKKLH*N*NQIQSHWNFLLPCPRF RIRHLVLPMEALDKVHLSLIVPVNIVFMWIVIWS MNSSWNW*KNFLLKTQKQRTHFLLRTQI*TWRC *LPISQWMMTSSYVPSISCHH*KAVPQALKAQV LKAQLQYSSRLKYKNLLLMPPLPLPPLMN*KQ*Q KTVWKTLKY*LHLHLLPTYIKKLLVPHHHHIEI LKVGQPHQTEQEKES*NRQKNLIQEALTCYLSL *VKELQFLRKN*IQRY*LCRMLRESEKWNMMVHF FKQ*ELEHYYSSQTIMQLLHHFLGNV*KDANLV NRMEWSKRQLF*YPLI*HVDCWGNQWMKVDYHS *PVMIVKLMLLYKAAETYCRVKNYSELWIKLTEL FLNFIPFFGHWWLIT*SSLFIFSTSNFRSLATI LHKLG*FNFDPLST*FTLMLFFSMFFNAGSQTA HFLSFLVFKPLHCSSIILKNAPFYLFIFG*GVYP FFELFLRRCQYNFCKKAVTFHHDHRQLKNFYTF FFTFYINNNALPAVRGSHNCTIYFLKKYQQLLM EYILRL*N*FLRRNFFWPMKLLNLEHDIVNHIIM ILKCCMVYYLNG*SHLHNIERYAYI*KVCGIYL DKILNSEKSSDVSIVTLPAQKKTIPYVVVEVYA NIV*LILNLNVLPTLLV*RYFEQTVNKKKKIMHS *QNCLVC*FAQNTMFDFMHFVAINILFFM*ISI IE*F*KHYFRNI*LSQ*ISCFFYVHCTNFSFLL LFVVGSNTNCIVLLHQINIFCGPGPFDQLLCSNI NNICFNTSNS*NCLPTI*ALMKITWYWLFLHLS *CHHLNVC*VPKCHVK**QP*YPIEKSMLLEKK HHF*VSEPIKMLKHKILVFLKYEWDE*SSSQHYS HNVTKARASLKISNLFPLTD**I*TSNE*FAQA LKVIDGRPKM*A*VS*L*SQT*NKFGLFLF**Y CVLKQSSFILFLLYFSFTK*LYFFYTDNLIFKNQ TV*KDIF*NRKITMNPCTYR