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Sequences

Sequence HSC20.dAug05

GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGT
GTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTGTAAGCTAAACA
GAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAA
GAAAAGATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCC
AGCACTTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAAT
TAGCCGGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAG
ATCACGCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
  • HSC20.u.f.8 (forward primer)
  • HSC20.u.r.10 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSG
CGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTL*AKQ
KEFTDNVSSAFEQDDFEEAKEILTKMRYFSNIE
EKIKLKKIPL*LWIV*SLKNKVLAGHSGSHL*S
QHFGRLRWVDDKVRSSRPAWPT**NPVSAENTKI
SRAWWRVPVIPATW*AEAGESLKPVRWRLQ*AE
ITQLHSSLGNRVRLNLEK*INEK*NSCIF

Frame 1

SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPG
VGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPCKLN
RKNLLTM*AVLLNKMTLKKPRKF*QR*DTFQI*K
KRSS*RRFPFNCG*FKV*KIKFLLGTVAHTCNP
STLGG*GGWMTRSGVQDQLGQHSETPSLLKIQK
LAGHGGACL*SQLLGRPRQENRLNP*GGGCSEQR
SRNCTPAWATE*DLILKNK*MKNKILVYF

Frame 2

LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRV
WGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLVS*T
ERIY*QCEQCF*TR*L*RSQGNFDKDEILFKYR
RKDQVKEDSPLIVDSLKFKK*SSCWAQWLTPVIP
ALWEAEVGG*QGQEFKTSLANIVKPRLC*KYKN
*PGMVARACNPSYLVGRGRRIA*TREVEVAVSR
DHATALQLGQQSET*S*KINK*KIKFLYI

Sequence HSC20.cAug05

GGTACTTGAGGGGCAGGGCCTGGGAGACTGGAAGACTTGAATGAATAGGCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCA
ACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAG
ACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATTCCTCA
TAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATCCATTGTCAAAG
CTAAACAGAAAGAATTTAC
TGACAATGTGAGCAGTGC
TTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAG
TTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGA
GGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGGGCATG
GTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCACGCAACTG
CACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
  • HSC20.u.f.8 (forward primer)
  • HSC20.u.r.10 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

GT*GAGPGRLEDLNE*ATVPSELIQRSSSTGTS
NCSVLSTQISSARGLRLKRTSQRSIRPW*MMPIR
PSWPP*AEDCTF*SSME*RFLKGQIMKWTGNSS
*K*WKSMKNSQKLKVKLP*KRLNPLSK
LNRKNL
LTM*AV
LLNKMTLKKPRKF*QR*DTFQI*KKRSS
*RRFPFNCG*FKV*KIKFLLGTVAHTCNPSTLG
G*GGWMTRSGVQDQLGQHSETPSLLKIQKLAGH
GGACL*SQLLGRPRQENRLNP*GGGCSEQRSRNC
TPAWATE*DLILKNK*MKNKILVYF

Frame 1

VLEGQGLGDWKT*MNRQPFLQS*YSEAPAQVPA
TAASCPPRFLQPEVSD*KGLLREAFDPGE*CL*
DPPGPPEQRTVPSKAPWNRDS*KDRL*NGQAIPH
RNNGNQ*KTRRS*K*SCHERD*IHCQS
*TERIY
*QCEQC
F*TR*L*RSQGNFDKDEILFKYRRKDQ
VKEDSPLIVDSLKFKK*SSCWAQWLTPVIPALWE
AEVGG*QGQEFKTSLANIVKPRLC*KYKN*PGM
VARACNPSYLVGRGRRIA*TREVEVAVSRDHAT
ALQLGQQSET*S*KINK*KIKFLYI

Frame 2

YLRGRAWETGRLE*IGNRSFRVDTAKLQHRYQQ
LQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYK
TLLAPLSRGLYLLKLHGIEIPERTDYEMDRQFL
IEIMEINEKLAEAESEAAMKEIESIVK
AKQKEFT
DNVSSA
FEQDDFEEAKEILTKMRYFSNIEEKIK
LKKIPL*LWIV*SLKNKVLAGHSGSHL*SQHFG
RLRWVDDKVRSSRPAWPT**NPVSAENTKISRAW
WRVPVIPATW*AEAGESLKPVRWRLQ*AEITQL
HSSLGNRVRLNLEK*INEK*NSCIF

Sequence HSC20.aAug05

GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGT
GTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTCTAAAGCTCCAT
GGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATTCCTCATAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAG
CTGCCATGAAAGAGATTGAATCCATTGTCAAAG
CTAAACAGAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAA
GGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAAT
AAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGC
CAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGA
ATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCACGCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATA
AATGAAAAATAAAATTCTTGTATATTTC
  • HSC20.u.f.8 (forward primer)
  • HSC20.u.r.10 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSG
CGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTF*SSM
E*RFLKGQIMKWTGNSS*K*WKSMKNSQKLKVK
LP*KRLNPLSK
LNRKNLLTM*AVLLNKMTLKKP
RKF*QR*DTFQI*KKRSS*RRFPFNCG*FKV*KI
KFLLGTVAHTCNPSTLGG*GGWMTRSGVQDQLG
QHSETPSLLKIQKLAGHGGACL*SQLLGRPRQE
NRLNP*GGGCSEQRSRNCTPAWATE*DLILKNK*
MKNKILVYF

Frame 1

SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPG
VGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPSKAP
WNRDS*KDRL*NGQAIPHRNNGNQ*KTRRS*K*S
CHERD*IHCQS
*TERIY*QCEQCF*TR*L*RSQ
GNFDKDEILFKYRRKDQVKEDSPLIVDSLKFKK
*SSCWAQWLTPVIPALWEAEVGG*QGQEFKTSLA
NIVKPRLC*KYKN*PGMVARACNPSYLVGRGRR
IA*TREVEVAVSRDHATALQLGQQSET*S*KIN
K*KIKFLYI

Frame 2

LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRV
WGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLLKLH
GIEIPERTDYEMDRQFLIEIMEINEKLAEAESE
AAMKEIESIVK
AKQKEFTDNVSSAFEQDDFEEAK
EILTKMRYFSNIEEKIKLKKIPL*LWIV*SLKN
KVLAGHSGSHL*SQHFGRLRWVDDKVRSSRPAW
PT**NPVSAENTKISRAWWRVPVIPATW*AEAGE
SLKPVRWRLQ*AEITQLHSSLGNRVRLNLEK*I
NEK*NSCIF

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