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Sequence HSC20.dAug05
GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTGTAAGCTAAACA
GAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAA
GAAAAGATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCC
AGCACTTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAAT
TAGCCGGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAG
ATCACGCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.8 (forward primer)
- HSC20.u.r.10 (reverse primer)
- Single exon skipping
Frame 0
VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSGCGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTL*AKQ
KEFTDNVSSAFEQDDFEEAKEILTKMRYFSNIE
EKIKLKKIPL*LWIV*SLKNKVLAGHSGSHL*S
QHFGRLRWVDDKVRSSRPAWPT**NPVSAENTKI
SRAWWRVPVIPATW*AEAGESLKPVRWRLQ*AE
ITQLHSSLGNRVRLNLEK*INEK*NSCIF
Frame 1
SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPGVGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPCKLN
RKNLLTM*AVLLNKMTLKKPRKF*QR*DTFQI*K
KRSS*RRFPFNCG*FKV*KIKFLLGTVAHTCNP
STLGG*GGWMTRSGVQDQLGQHSETPSLLKIQK
LAGHGGACL*SQLLGRPRQENRLNP*GGGCSEQR
SRNCTPAWATE*DLILKNK*MKNKILVYF
Frame 2
LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLVS*T
ERIY*QCEQCF*TR*L*RSQGNFDKDEILFKYR
RKDQVKEDSPLIVDSLKFKK*SSCWAQWLTPVIP
ALWEAEVGG*QGQEFKTSLANIVKPRLC*KYKN
*PGMVARACNPSYLVGRGRRIA*TREVEVAVSR
DHATALQLGQQSET*S*KINK*KIKFLYI
Sequence HSC20.gAug05
GGTACTTGAGGGGCAGGGCCTGGGAGACTGGAAGACTTGAATGAATAGGCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAG
ACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTGTAAGCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATT
CCTCATAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATCCATTGTCAAAGCTAAACAGAAAGAA
TTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGA
TCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTT
TGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGG
GCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCACGC
AACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.8 (forward primer)
- HSC20.u.r.10 (reverse primer)
- Single exon skipping
Frame 0
GT*GAGPGRLEDLNE*ATVPSELIQRSSSTGTSNCSVLSTQISSARGLRLKRTSQRSIRPW*MMPIR
PSWPP*AEDCTL*AKAPWNRDS*KDRL*NGQAI
PHRNNGNQ*KTRRS*K*SCHERD*IHCQS*TER
IY*QCEQCF*TR*L*RSQGNFDKDEILFKYRRKD
QVKEDSPLIVDSLKFKK*SSCWAQWLTPVIPAL
WEAEVGG*QGQEFKTSLANIVKPRLC*KYKN*P
GMVARACNPSYLVGRGRRIA*TREVEVAVSRDHA
TALQLGQQSET*S*KINK*KIKFLYI
Frame 1
VLEGQGLGDWKT*MNRQPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD*KGLLREAFDPGE*CL*
DPPGPPEQRTVPCKLKLHGIEIPERTDYEMDRQF
LIEIMEINEKLAEAESEAAMKEIESIVKAKQKE
FTDNVSSAFEQDDFEEAKEILTKMRYFSNIEEK
IKLKKIPL*LWIV*SLKNKVLAGHSGSHL*SQHF
GRLRWVDDKVRSSRPAWPT**NPVSAENTKISR
AWWRVPVIPATW*AEAGESLKPVRWRLQ*AEIT
QLHSSLGNRVRLNLEK*INEK*NSCIF
Frame 2
YLRGRAWETGRLE*IGNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYK
TLLAPLSRGLYLVS*SSME*RFLKGQIMKWTGN
SS*K*WKSMKNSQKLKVKLP*KRLNPLSKLNRKN
LLTM*AVLLNKMTLKKPRKF*QR*DTFQI*KKR
SS*RRFPFNCG*FKV*KIKFLLGTVAHTCNPST
LGG*GGWMTRSGVQDQLGQHSETPSLLKIQKLAG
HGGACL*SQLLGRPRQENRLNP*GGGCSEQRSR
NCTPAWATE*DLILKNK*MKNKILVYF
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