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Sequence HSC20.fAug05
GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTCTAAACAGAAAG
AATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAA
GATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCAC
TTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCC
GGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCAC
GCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.8 (forward primer)
- HSC20.u.r.10 (reverse primer)
- Single exon skipping
Frame 0
VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSGCGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTF*TER
IY*QCEQCF*TR*L*RSQGNFDKDEILFKYRRK
DQVKEDSPLIVDSLKFKK*SSCWAQWLTPVIPA
LWEAEVGG*QGQEFKTSLANIVKPRLC*KYKN*P
GMVARACNPSYLVGRGRRIA*TREVEVAVSRDH
ATALQLGQQSET*S*KINK*KIKFLYI
Frame 1
SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPGVGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPSKQK
EFTDNVSSAFEQDDFEEAKEILTKMRYFSNIEEK
IKLKKIPL*LWIV*SLKNKVLAGHSGSHL*SQH
FGRLRWVDDKVRSSRPAWPT**NPVSAENTKIS
RAWWRVPVIPATW*AEAGESLKPVRWRLQ*AEIT
QLHSSLGNRVRLNLEK*INEK*NSCIF
Frame 2
LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLLNRK
NLLTM*AVLLNKMTLKKPRKF*QR*DTFQI*KK
RSS*RRFPFNCG*FKV*KIKFLLGTVAHTCNPST
LGG*GGWMTRSGVQDQLGQHSETPSLLKIQKLA
GHGGACL*SQLLGRPRQENRLNP*GGGCSEQRS
RNCTPAWATE*DLILKNK*MKNKILVYF
Sequence HSC20.cAug05
GGTACTTGAGGGGCAGGGCCTGGGAGACTGGAAGACTTGAATGAATAGGCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAG
ACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATTCCTCA
TAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATCCATTGTCAAAGCTAAACAGAAAGAATTTAC
TGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAG
TTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGA
GGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGGGCATG
GTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCACGCAACTG
CACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.8 (forward primer)
- HSC20.u.r.10 (reverse primer)
- Single exon skipping
Frame 0
GT*GAGPGRLEDLNE*ATVPSELIQRSSSTGTSNCSVLSTQISSARGLRLKRTSQRSIRPW*MMPIR
PSWPP*AEDCTF*SSME*RFLKGQIMKWTGNSS
*K*WKSMKNSQKLKVKLP*KRLNPLSKLNRKNL
LTM*AVLLNKMTLKKPRKF*QR*DTFQI*KKRSS
*RRFPFNCG*FKV*KIKFLLGTVAHTCNPSTLG
G*GGWMTRSGVQDQLGQHSETPSLLKIQKLAGH
GGACL*SQLLGRPRQENRLNP*GGGCSEQRSRNC
TPAWATE*DLILKNK*MKNKILVYF
Frame 1
VLEGQGLGDWKT*MNRQPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD*KGLLREAFDPGE*CL*
DPPGPPEQRTVPSKAPWNRDS*KDRL*NGQAIPH
RNNGNQ*KTRRS*K*SCHERD*IHCQS*TERIY
*QCEQCF*TR*L*RSQGNFDKDEILFKYRRKDQ
VKEDSPLIVDSLKFKK*SSCWAQWLTPVIPALWE
AEVGG*QGQEFKTSLANIVKPRLC*KYKN*PGM
VARACNPSYLVGRGRRIA*TREVEVAVSRDHAT
ALQLGQQSET*S*KINK*KIKFLYI
Frame 2
YLRGRAWETGRLE*IGNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYK
TLLAPLSRGLYLLKLHGIEIPERTDYEMDRQFL
IEIMEINEKLAEAESEAAMKEIESIVKAKQKEFT
DNVSSAFEQDDFEEAKEILTKMRYFSNIEEKIK
LKKIPL*LWIV*SLKNKVLAGHSGSHL*SQHFG
RLRWVDDKVRSSRPAWPT**NPVSAENTKISRAW
WRVPVIPATW*AEAGESLKPVRWRLQ*AEITQL
HSSLGNRVRLNLEK*INEK*NSCIF
Sequence HSC20.aAug05
GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTCTAAAGCTCCAT
GGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATTCCTCATAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAG
CTGCCATGAAAGAGATTGAATCCATTGTCAAAGCTAAACAGAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAA
GGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAAT
AAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGC
CAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGA
ATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCACGCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATA
AATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.8 (forward primer)
- HSC20.u.r.10 (reverse primer)
- Single exon skipping
Frame 0
VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSGCGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTF*SSM
E*RFLKGQIMKWTGNSS*K*WKSMKNSQKLKVK
LP*KRLNPLSKLNRKNLLTM*AVLLNKMTLKKP
RKF*QR*DTFQI*KKRSS*RRFPFNCG*FKV*KI
KFLLGTVAHTCNPSTLGG*GGWMTRSGVQDQLG
QHSETPSLLKIQKLAGHGGACL*SQLLGRPRQE
NRLNP*GGGCSEQRSRNCTPAWATE*DLILKNK*
MKNKILVYF
Frame 1
SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPGVGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPSKAP
WNRDS*KDRL*NGQAIPHRNNGNQ*KTRRS*K*S
CHERD*IHCQS*TERIY*QCEQCF*TR*L*RSQ
GNFDKDEILFKYRRKDQVKEDSPLIVDSLKFKK
*SSCWAQWLTPVIPALWEAEVGG*QGQEFKTSLA
NIVKPRLC*KYKN*PGMVARACNPSYLVGRGRR
IA*TREVEVAVSRDHATALQLGQQSET*S*KIN
K*KIKFLYI
Frame 2
LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLLKLH
GIEIPERTDYEMDRQFLIEIMEINEKLAEAESE
AAMKEIESIVKAKQKEFTDNVSSAFEQDDFEEAK
EILTKMRYFSNIEEKIKLKKIPL*LWIV*SLKN
KVLAGHSGSHL*SQHFGRLRWVDDKVRSSRPAW
PT**NPVSAENTKISRAWWRVPVIPATW*AEAGE
SLKPVRWRLQ*AEITQLHSSLGNRVRLNLEK*I
NEK*NSCIF
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