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Sequence HSC20.fAug05
GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTCTAAACAGAAAG
AATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAA
GATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCAC
TTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCC
GGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCAC
GCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.8 (forward primer)
- HSC20.u.r.10 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSGCGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTF*TER
IY*QCEQCF*TR*L*RSQGNFDKDEILFKYRRK
DQVKEDSPLIVDSLKFKK*SSCWAQWLTPVIPA
LWEAEVGG*QGQEFKTSLANIVKPRLC*KYKN*P
GMVARACNPSYLVGRGRRIA*TREVEVAVSRDH
ATALQLGQQSET*S*KINK*KIKFLYI
Frame 1
SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPGVGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPSKQK
EFTDNVSSAFEQDDFEEAKEILTKMRYFSNIEEK
IKLKKIPL*LWIV*SLKNKVLAGHSGSHL*SQH
FGRLRWVDDKVRSSRPAWPT**NPVSAENTKIS
RAWWRVPVIPATW*AEAGESLKPVRWRLQ*AEIT
QLHSSLGNRVRLNLEK*INEK*NSCIF
Frame 2
LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLLNRK
NLLTM*AVLLNKMTLKKPRKF*QR*DTFQI*KK
RSS*RRFPFNCG*FKV*KIKFLLGTVAHTCNPST
LGG*GGWMTRSGVQDQLGQHSETPSLLKIQKLA
GHGGACL*SQLLGRPRQENRLNP*GGGCSEQRS
RNCTPAWATE*DLILKNK*MKNKILVYF
Sequence HSC20.gAug05
GGTACTTGAGGGGCAGGGCCTGGGAGACTGGAAGACTTGAATGAATAGGCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAG
ACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTGTAAGCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATT
CCTCATAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATCCATTGTCAAAGCTAAACAGAAAGAA
TTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGA
TCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTT
TGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGG
GCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCACGC
AACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.8 (forward primer)
- HSC20.u.r.10 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
GT*GAGPGRLEDLNE*ATVPSELIQRSSSTGTSNCSVLSTQISSARGLRLKRTSQRSIRPW*MMPIR
PSWPP*AEDCTL*AKAPWNRDS*KDRL*NGQAI
PHRNNGNQ*KTRRS*K*SCHERD*IHCQS*TER
IY*QCEQCF*TR*L*RSQGNFDKDEILFKYRRKD
QVKEDSPLIVDSLKFKK*SSCWAQWLTPVIPAL
WEAEVGG*QGQEFKTSLANIVKPRLC*KYKN*P
GMVARACNPSYLVGRGRRIA*TREVEVAVSRDHA
TALQLGQQSET*S*KINK*KIKFLYI
Frame 1
VLEGQGLGDWKT*MNRQPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD*KGLLREAFDPGE*CL*
DPPGPPEQRTVPCKLKLHGIEIPERTDYEMDRQF
LIEIMEINEKLAEAESEAAMKEIESIVKAKQKE
FTDNVSSAFEQDDFEEAKEILTKMRYFSNIEEK
IKLKKIPL*LWIV*SLKNKVLAGHSGSHL*SQHF
GRLRWVDDKVRSSRPAWPT**NPVSAENTKISR
AWWRVPVIPATW*AEAGESLKPVRWRLQ*AEIT
QLHSSLGNRVRLNLEK*INEK*NSCIF
Frame 2
YLRGRAWETGRLE*IGNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYK
TLLAPLSRGLYLVS*SSME*RFLKGQIMKWTGN
SS*K*WKSMKNSQKLKVKLP*KRLNPLSKLNRKN
LLTM*AVLLNKMTLKKPRKF*QR*DTFQI*KKR
SS*RRFPFNCG*FKV*KIKFLLGTVAHTCNPST
LGG*GGWMTRSGVQDQLGQHSETPSLLKIQKLAG
HGGACL*SQLLGRPRQENRLNP*GGGCSEQRSR
NCTPAWATE*DLILKNK*MKNKILVYF
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