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Sequence HSC20.fAug05
GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTCTAAACAGAAAG
AATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAA
GATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAGCAC
TTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCC
GGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGATCAC
GCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.8 (forward primer)
- HSC20.u.r.10 (reverse primer)
- Alternative donor
Frame 0
VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSGCGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTF*TER
IY*QCEQCF*TR*L*RSQGNFDKDEILFKYRRK
DQVKEDSPLIVDSLKFKK*SSCWAQWLTPVIPA
LWEAEVGG*QGQEFKTSLANIVKPRLC*KYKN*P
GMVARACNPSYLVGRGRRIA*TREVEVAVSRDH
ATALQLGQQSET*S*KINK*KIKFLYI
Frame 1
SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPGVGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPSKQK
EFTDNVSSAFEQDDFEEAKEILTKMRYFSNIEEK
IKLKKIPL*LWIV*SLKNKVLAGHSGSHL*SQH
FGRLRWVDDKVRSSRPAWPT**NPVSAENTKIS
RAWWRVPVIPATW*AEAGESLKPVRWRLQ*AEIT
QLHSSLGNRVRLNLEK*INEK*NSCIF
Frame 2
LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLLNRK
NLLTM*AVLLNKMTLKKPRKF*QR*DTFQI*KK
RSS*RRFPFNCG*FKV*KIKFLLGTVAHTCNPST
LGG*GGWMTRSGVQDQLGQHSETPSLLKIQKLA
GHGGACL*SQLLGRPRQENRLNP*GGGCSEQRS
RNCTPAWATE*DLILKNK*MKNKILVYF
Sequence HSC20.dAug05
GTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGCCAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGC
GGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACT
GCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGAC
TGAAAAGGACTTCTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTACCTTGTAAGCTAAACA
GAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAA
GAAAAGATCAAGTTAAAGAAGATTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCC
AGCACTTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCAACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAAT
TAGCCGGGCATGGTGGCGCGTGCCTGTAATCCCAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAG
ATCACGCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAAAATTCTTGTATATTTC
- HSC20.u.f.8 (forward primer)
- HSC20.u.r.10 (reverse primer)
- Alternative donor
Frame 0
VWLDALFAFPHE*PRLDRPPARCGGGEPGLCSGCGGFGRQGFPEGDR*AAMLRRRREAIIPAVGTAA
AHGAPGGRTGSSAHSAERCRHLTPLETTSALWT
ATVPSELIQRSSSTGTSNCSVLSTQISSARGLR
LKRTSQRSIRPW*MMPIRPSWPP*AEDCTL*AKQ
KEFTDNVSSAFEQDDFEEAKEILTKMRYFSNIE
EKIKLKKIPL*LWIV*SLKNKVLAGHSGSHL*S
QHFGRLRWVDDKVRSSRPAWPT**NPVSAENTKI
SRAWWRVPVIPATW*AEAGESLKPVRWRLQ*AE
ITQLHSSLGNRVRLNLEK*INEK*NSCIF
Frame 1
SG*TLSLLFPTSDHG*IGRRPDVAGESRGFAPGVGVLADRGSQKETAKLRCCVAGGKQLSPLLELR
RPMGPRAGGQVLLPTVPSAAGT*PHSRLLQPYGL
QPFLQS*YSEAPAQVPATAASCPPRFLQPEVSD
*KGLLREAFDPGE*CL*DPPGPPEQRTVPCKLN
RKNLLTM*AVLLNKMTLKKPRKF*QR*DTFQI*K
KRSS*RRFPFNCG*FKV*KIKFLLGTVAHTCNP
STLGG*GGWMTRSGVQDQLGQHSETPSLLKIQK
LAGHGGACL*SQLLGRPRQENRLNP*GGGCSEQR
SRNCTPAWATE*DLILKNK*MKNKILVYF
Frame 2
LVRRSLCFSPRVTTAR*AAGQMWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCG
GPWGPGREDRFFCPQCRALQAPDPTRDYFSLMD
CNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQT
EKDFSEKHSTLVNDAYKTLLAPLSRGLYLVS*T
ERIY*QCEQCF*TR*L*RSQGNFDKDEILFKYR
RKDQVKEDSPLIVDSLKFKK*SSCWAQWLTPVIP
ALWEAEVGG*QGQEFKTSLANIVKPRLC*KYKN
*PGMVARACNPSYLVGRGRRIA*TREVEVAVSR
DHATALQLGQQSET*S*KINK*KIKFLYI
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