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Sequence MMP2.eAug05
TGGGGTGACGAGGTCGTGCACTGAGGGTGGACGTAGAGGCCAGGAGTAGCAGGCGGCCGGGGAAAAGAGGTGGAGAAAGGAAAAAAGAGGAGAAAAGTGGAGGAGGGCGAGTAGGGGGGTGGGGCAGAGAGGGGCGGGCCCGAGTGCGCCCCCCGCCCCCAGCCCCGCTCTGCCAGCTCCCTCCCAGCCCAGCCGGCTAC
ATCTGGCGGCTGCCCTCCCTTGTTTCCGCTGCATCCAGACTTCCTCAGGCGGTGGCTGGAGGCTGCGCATCTGGGGCTTTAAACATACAAAGGGATTGCC
AGGACCCGCCGCGCCGTCGCCCATCATCAAGTTCCCCGGCGATGTCGCCCCCAAAACGGACAAAGAGTTGGCAGTGCAATACCTGAACACCTTCTATGGC
TGCCCCAAGGAGAGCTGCAACCTGTTTGTGCTGAAGGACACACTAAAGAAGATGCAGAAGTTCTTTGGACTGCCCCAGACAGGTGATCTTGACCAGAATA
CCATCGAGACCATGCGGAAGCCACGCTGCGGCAACCCAGATGTGGCCAACTACAACTTCTTCCCTCGCAAGCCCAAGTGGGACAAGAACCAGATCACATA
CAGGATCATTGGCTACACACCTGATCTGGACCCAGAGACAGTGGATGATGCCTTTGCTCGTGCCTTCCAAGTCTGGAGCGATGTGACCCCACTGCGGTTT
TCTCGAATCCATGATGGAGAGGCAGACATCATGATCAACTTTGGCCGCTGGGAGCATGGCGATGGATACCCCTTTGACGGTAAGGACGGACTCCTGGCTC
ATGCCTTCGCCCCAGGCACTGGTGTTGGGGGAGACTCCCATTTTGATGACGATGAGCTATGGACCTTGGGAGAAGGCCAAGTGGTCCGTGTGAAGTATGG
GAACGCCGATGGGGAGTACTGCAAGTTCCCCTTCTTGTTCAATGGCAAGGAGTACAACAGCTGCACTGATACCGGCCGCAGCGATGGCTTCCTCTGGTGC
TCCACCACCTACAACTTTG
- MMP2.u.f.4 (forward primer)
- MMP2.u.r.3 (reverse primer)
- Alternative donor
Frame 0
WGDEVVH*GWT*RPGVAGGRGKEVEKGKKRRKVEEGE*GGGAERGGPECAPRPQPRSASSLPAQPAT
SGGCPPLFPLHPDFLRRWLEAAHLGL*TYKGIA
RTRRAVAHHQVPRRCRPQNGQRVGSAIPEHLLW
LPQGELQPVCAEGHTKEDAEVLWTAPDR*S*PEY
HRDHAEATLRQPRCGQLQLLPSQAQVGQEPDHI
QDHWLHT*SGPRDSG*CLCSCLPSLERCDPTAV
FSNP*WRGRHHDQLWPLGAWRWIPL*R*GRTPGS
CLRPRHWCWGRLPF**R*AMDLGRRPSGPCEVW
ERRWGVLQVPLLVQWQGVQQLH*YRPQRWLPLV
LHHLQL
Frame 1
GVTRSCTEGGRRGQE*QAAGEKRWRKEKRGEKWRRASRGVGQRGAGPSAPPAPSPALPAPSQPSRL
HLAAALPCFRCIQTSSGGGWRLRIWGFKHTKGLP
GPAAPSPIIKFPGDVAPKTDKELAVQYLNTFYG
CPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQN
TIETMRKPRCGNPDVANYNFFPRKPKWDKNQITY
RIIGYTPDLDPETVDDAFARAFQVWSDVTPLRF
SRIHDGEADIMINFGRWEHGDGYPFDGKDGLLA
HAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYG
NADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWC
STTYNF
Frame 2
G*RGRALRVDVEARSSRRPGKRGGERKKEEKSGGGRVGGWGREGRARVRPPPPAPLCQLPPSPAGY
IWRLPSLVSAASRLPQAVAGGCASGALNIQRDC
QDPPRRRPSSSSPAMSPPKRTKSWQCNT*TPSMA
APRRAATCLC*RTH*RRCRSSLDCPRQVILTRI
PSRPCGSHAAATQMWPTTTSSLASPSGTRTRSH
TGSLATHLIWTQRQWMMPLLVPSKSGAM*PHCGF
LESMMERQTS*STLAAGSMAMDTPLTVRTDSWL
MPSPQALVLGETPILMTMSYGPWEKAKWSV*SM
GTPMGSTASSPSCSMARSTTAALIPAAAMASSGA
PPPTTL
Sequence MMP2.cAug05
TGGGGTGACGAGGTCGTGCACTGAGGGTGGACGTAGAGGCCAGGAGTAGCAGGCGGCCGGGGAAAAGAGGTGGAGAAAGGAAAAAAGAGGAGAAAAGTGGAGGAGGGCGAGTAGGGGGGTGGGGCAGAGAGGGGCGGGCCCGAGTGCGCCCCCCGCCCCCAGCCCCGCTCTGCCAGCTCCCTCCCAGCCCAGCCGGCTAC
ATCTGGCGGCTGCCCTCCCTTGTTTCCGCTGCATCCAGACTTCCTCAGGCGGTGGCTGGAGGCTGCGCATCTGGGGCTTTAAACATACAAAGGGATTGCC
AGGACCTGCGCCGCCGCGCCGTCGCCCATCATCAAGTTCCCCGGCGATGTCGCCCCCAAAACGGACAAAGAGTTGGCAGTGCAATACCTGAACACCTTCT
ATGGCTGCCCCAAGGAGAGCTGCAACCTGTTTGTGCTGAAGGACACACTAAAGAAGATGCAGAAGTTCTTTGGACTGCCCCAGACAGGTGATCTTGACCA
GAATACCATCGAGACCATGCGGAAGCCACGCTGCGGCAACCCAGATGTGGCCAACTACAACTTCTTCCCTCGCAAGCCCAAGTGGGACAAGAACCAGATC
ACATACAGGATCATTGGCTACACACCTGATCTGGACCCAGAGACAGTGGATGATGCCTTTGCTCGTGCCTTCCAAGTCTGGAGCGATGTGACCCCACTGC
GGTTTTCTCGAATCCATGATGGAGAGGCAGACATCATGATCAACTTTGGCCGCTGGGAGCATGGCGATGGATACCCCTTTGACGGTAAGGACGGACTCCT
GGCTCATGCCTTCGCCCCAGGCACTGGTGTTGGGGGAGACTCCCATTTTGATGACGATGAGCTATGGACCTTGGGAGAAGGCCAAGTGGTCCGTGTGAAG
TATGGGAACGCCGATGGGGAGTACTGCAAGTTCCCCTTCTTGTTCAATGGCAAGGAGTACAACAGCTGCACTGATACCGGCCGCAGCGATGGCTTCCTCT
GGTGCTCCACCACCTACAACTTTGAGAAGGATGGCAAGTACGGCTTCTGTCCCCATGAAGCCCTGTTCACCATGGGCGGCAACGCTGAAGGACAGCCCTG
CAAGTTTCCATTCCGCTTCCAGGGCACATCCTATGACAGCTGCACCACTGAGGGCCGCACGGATGGCTACCGCTGGTGCGGCACCACTGAGGACTACGAC
CGCGACAAGAAGTATGGCTTCTGCCCTGAGACCGGTGGGTGCCACTCCCTCTCCCTCCCTCAGGGCCCAGCACCTGCTGTCTGACAAAAAAAAAA
- MMP2.u.f.4 (forward primer)
- MMP2.u.r.3 (reverse primer)
- Alternative donor
Frame 0
WGDEVVH*GWT*RPGVAGGRGKEVEKGKKRRKVEEGE*GGGAERGGPECAPRPQPRSASSLPAQPAT
SGGCPPLFPLHPDFLRRWLEAAHLGL*TYKGIA
RTCAAAPSPIIKFPGDVAPKTDKELAVQYLNTF
YGCPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQ
NTIETMRKPRCGNPDVANYNFFPRKPKWDKNQI
TYRIIGYTPDLDPETVDDAFARAFQVWSDVTPL
RFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLL
AHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVK
YGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFL
WCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPC
KFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYD
RDKKYGFCPETGGCHSLSLPQGPAPAV*QKK
Frame 1
GVTRSCTEGGRRGQE*QAAGEKRWRKEKRGEKWRRASRGVGQRGAGPSAPPAPSPALPAPSQPSRL
HLAAALPCFRCIQTSSGGGWRLRIWGFKHTKGLP
GPAPPRRRPSSSSPAMSPPKRTKSWQCNT*TPS
MAAPRRAATCLC*RTH*RRCRSSLDCPRQVILT
RIPSRPCGSHAAATQMWPTTTSSLASPSGTRTRS
HTGSLATHLIWTQRQWMMPLLVPSKSGAM*PHC
GFLESMMERQTS*STLAAGSMAMDTPLTVRTDS
WLMPSPQALVLGETPILMTMSYGPWEKAKWSV*S
MGTPMGSTASSPSCSMARSTTAALIPAAAMASS
GAPPPTTLRRMASTASVPMKPCSPWAATLKDSP
ASFHSASRAHPMTAAPLRAARMATAGAAPLRTTT
ATRSMASALRPVGATPSPSLRAQHLLSDKKK
Frame 2
G*RGRALRVDVEARSSRRPGKRGGERKKEEKSGGGRVGGWGREGRARVRPPPPAPLCQLPPSPAGY
IWRLPSLVSAASRLPQAVAGGCASGALNIQRDC
QDLRRRAVAHHQVPRRCRPQNGQRVGSAIPEHLL
WLPQGELQPVCAEGHTKEDAEVLWTAPDR*S*P
EYHRDHAEATLRQPRCGQLQLLPSQAQVGQEPD
HIQDHWLHT*SGPRDSG*CLCSCLPSLERCDPTA
VFSNP*WRGRHHDQLWPLGAWRWIPL*R*GRTP
GSCLRPRHWCWGRLPF**R*AMDLGRRPSGPCE
VWERRWGVLQVPLLVQWQGVQQLH*YRPQRWLPL
VLHHLQL*EGWQVRLLSP*SPVHHGRQR*RTAL
QVSIPLPGHIL*QLHH*GPHGWLPLVRHH*GLR
PRQEVWLLP*DRWVPLPLPPSGPSTCCLTKKK
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