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Sequences

Sequence MMP2.cAug05

MMP2.u.f.4 (forward primer)
MMP2.u.r.3 (reverse primer)
Intron retention

Frame 0

WGDEVVH*GWT*RPGVAGGRGKEVEKGKKRRKV EEGE*GGGAERGGPECAPRPQPRSASSLPAQPAT SGGCPPLFPLHPDFLRRWLEAAHLGL*TYKGIA RTCAAAPSPIIKFPGDVAPKTDKELAVQYLNTF YGCPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQ NTIETMRKPRCGNPDVANYNFFPRKPKWDKNQI TYRIIGYTPDLDPETVDDAFARAFQVWSDVTPL RFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLL AHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVK YGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFL WCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPC KFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYD RDKKYGFCPETGGCHSLSLPQGPAPAV*QKK

Frame 1

GVTRSCTEGGRRGQE*QAAGEKRWRKEKRGEKW RRASRGVGQRGAGPSAPPAPSPALPAPSQPSRL HLAAALPCFRCIQTSSGGGWRLRIWGFKHTKGLP GPAPPRRRPSSSSPAMSPPKRTKSWQCNT*TPS MAAPRRAATCLC*RTH*RRCRSSLDCPRQVILT RIPSRPCGSHAAATQMWPTTTSSLASPSGTRTRS HTGSLATHLIWTQRQWMMPLLVPSKSGAM*PHC GFLESMMERQTS*STLAAGSMAMDTPLTVRTDS WLMPSPQALVLGETPILMTMSYGPWEKAKWSV*S MGTPMGSTASSPSCSMARSTTAALIPAAAMASS GAPPPTTLRRMASTASVPMKPCSPWAATLKDSP ASFHSASRAHPMTAAPLRAARMATAGAAPLRTTT ATRSMASALRPVGATPSPSLRAQHLLSDKKK

Frame 2

G*RGRALRVDVEARSSRRPGKRGGERKKEEKSG GGRVGGWGREGRARVRPPPPAPLCQLPPSPAGY IWRLPSLVSAASRLPQAVAGGCASGALNIQRDC QDLRRRAVAHHQVPRRCRPQNGQRVGSAIPEHLL WLPQGELQPVCAEGHTKEDAEVLWTAPDR*S*P EYHRDHAEATLRQPRCGQLQLLPSQAQVGQEPD HIQDHWLHT*SGPRDSG*CLCSCLPSLERCDPTA VFSNP*WRGRHHDQLWPLGAWRWIPL*R*GRTP GSCLRPRHWCWGRLPF**R*AMDLGRRPSGPCE VWERRWGVLQVPLLVQWQGVQQLH*YRPQRWLPL VLHHLQL*EGWQVRLLSP*SPVHHGRQR*RTAL QVSIPLPGHIL*QLHH*GPHGWLPLVRHH*GLR PRQEVWLLP*DRWVPLPLPPSGPSTCCLTKKK

Sequence MMP2.aAug05

TGGGGTGACGAGGTCGTGCACTGAGGGTGGACGTAGAGGCCAGGAGTAGCAGGCGGCCGGGGAAAAGAGGTGGAGAAAGGAAAAAAGAGGAGAAAAGTGG AGGAGGGCGAGTAGGGGGGTGGGGCAGAGAGGGGCGGGCCCGAGTGCGCCCCCCGCCCCCAGCCCCGCTCTGCCAGCTCCCTCCCAGCCCAGCCGGCTAC ATCTGGCGGCTGCCCTCCCTTGTTTCCGCTGCATCCAGACTTCCTCAGGCGGTGGCTGGAGGCTGCGCATCTGGGGCTTTAAACATACAAAGGGATTGCC AGGACCTGCGGCGGCGGCGGCGGCGGCGGGGGCTGGGGCGCGGGGGCCGGACCATGAGCCGCTGAGCCGGGCAAACCCCAGGCCACCGAGCCAGCGGACC CTCGGAGCGCAGCCCTGCGCCGCGGAGCAGGCTCCAACCAGGCGGCGAGGCGGCCACACGCACCGAGCCAGCGACCCCCGGGCGACGCGCGGGGCCAGGG AGCGCTACGATGGAGGCGCTAATGGCCCGGGGCGCGCTCACGGGTCCCCTGAGGGCGCTCTGTCTCCTGGGCTGCCTGCTGAGCCACGCCGCCGCCGCGC CGTCGCCCATCATCAAGTTCCCCGGCGATGTCGCCCCCAAAACGGACAAAGAGTTGGCAGTGCAATACCTGAACACCTTCTATGGCTGCCCCAAGGAGAG CTGCAACCTGTTTGTGCTGAAGGACACACTAAAGAAGATGCAGAAGTTCTTTGGACTGCCCCAGACAGGTGATCTTGACCAGAATACCATCGAGACCATG CGGAAGCCACGCTGCGGCAACCCAGATGTGGCCAACTACAACTTCTTCCCTCGCAAGCCCAAGTGGGACAAGAACCAGATCACATACAGGATCATTGGCT ACACACCTGATCTGGACCCAGAGACAGTGGATGATGCCTTTGCTCGTGCCTTCCAAGTCTGGAGCGATGTGACCCCACTGCGGTTTTCTCGAATCCATGA TGGAGAGGCAGACATCATGATCAACTTTGGCCGCTGGGAGCATGGCGATGGATACCCCTTTGACGGTAAGGACGGACTCCTGGCTCATGCCTTCGCCCCA GGCACTGGTGTTGGGGGAGACTCCCATTTTGATGACGATGAGCTATGGACCTTGGGAGAAGGCCAAGTGGTCCGTGTGAAGTATGGGAACGCCGATGGGG AGTACTGCAAGTTCCCCTTCTTGTTCAATGGCAAGGAGTACAACAGCTGCACTGATACCGGCCGCAGCGATGGCTTCCTCTGGTGCTCCACCACCTACAA CTTTGAGAAGGATGGCAAGTACGGCTTCTGTCCCCATGAAGCCCTGTTCACCATGGGCGGCAACGCTGAAGGACAGCCCTGCAAGTTTCCATTCCGCTTC CAGGGCACATCCTATGACAGCTGCACCACTGAGGGCCGCACGGATGGCTACCGCTGGTGCGGCACCACTGAGGACTACGACCGCGACAAGAAGTATGGCT TCTGCCCTGAGACCGCCATGTCCACTGTTGGTGGGAACTCAGAAGGTGCCCCCTGTGTCTTCCCCTTCACTTTCCTGGGCAACAAATATGAGAGCTGCAC CAGCGCCGGCCGCAGTGACGGAAAGATGTGGTGTGCGACCACAGCCAACTACGATGATGACCGCAAGTGGGGCTTCTGCCCTGACCAAGGGTACAGCCTG TTCCTCGTGGCAGCCCACGAGTTTGGCCACGCCATGGGGCTGGAGCACTCCCAAGACCCTGGGGCCCTGATGGCACCCATTTACACCTACACCAAGAACT TCCGTCTGTCCCAGGATGACATCAAGGGCATTCAGGAGCTCTATGGGGCCTCTCCTGACATTGACCTTGGCACCGGCCCCACCCCCACGCTGGGCCCTGT CACTCCTGAGATCTGCAAACAGGACATTGTATTTGATGGCATCGCTCAGATCCGTGGTGAGATCTTCTTCTTCAAGGACCGGTTCATTTGGCGGACTGTG ACGCCACGTGACAAGCCCATGGGGCCCCTGCTGGTGGCCACATTCTGGCCTGAGCTCCCGGAAAAGATTGATGCGGTATACGAGGCCCCACAGGAGGAGA AGGCTGTGTTCTTTGCAGGGAATGAATACTGGATCTACTCAGCCAGCACCCTGGAGCGAGGGTACCCCAAGCCACTGACCAGCCTGGGACTGCCCCCTGA TGTCCAGCGAGTGGATGCCGCCTTTAACTGGAGCAAAAACAAGAAGACATACATCTTTGCTGGAGACAAATTCTGGAGATACAATGAGGTGAAGAAGAAA ATGGATCCTGGCTTCCCCAAGCTCATCGCAGATGCCTGGAATGCCATCCCCGATAACCTGGATGCCGTCGTGGACCTGCAGGGCGGCGGTCACAGCTACT TCTTCAAGGGTGCCTATTACCTGAAGCTGGAGAACCAAAGTCTGAAGAGCGTGAAGTTTGGAAGCATCAAATCCGACTGGCTAGGCTGCTGAGCTGGCCC TGGCTCCCACAGGCCCTTCCTCTCCACTGCCTTCGATACACCGGGCCTGGAGAACTAGAGAAGGACCCGGAGGGGCCTGGCAGCCGTGCCTTCAGCTCTA CAGCTAATCAGCATTCTCACTCCTACCTGGTAATTTAAGATTCCAGAGAGTGGCTCCTCCCGGTGCCCAAGAATAGATGCTGACTGTACTCCTCCCAGGC GCCCCTTCCCCCTCCAATCCCACCAACCCTCAGAGCCACCCCTAAAGAGATACTTTGATATTTTCAACGCAGCCCTGCTTTGGGCTGCCCTGGTGCTGCC ACACTTCAGGCTCTTCTCCTTTCACAACCTTCTGTGGCTCACAGAACCCTTGGAGCCAATGGAGACTGTCTCAAGAGGGCACTGGTGGCCCGACAGCCTG GCACAGGGCAGTGGGACAGGGCATGGCCAGGTGGCCACTCCAGACCCCTGGCTTTTCACTGCTGGCTGCCTTAGAACCTTTCTTACATTAGCAGTTTGCT TTGTATGCACTTTGTTTTTTTCTTTGGGTCTTGTTTTTTTTTTCCACTTAGAAATTGCATTTCCTGACAGAAGGACTCAGGTTGTCTGAAGTCACTGCAC AGTGCATCTCAGCCCACATAGTGATGGTTCCCCTGTTCACTCTACTTAGCATGTCCCTACCGAGTCTCTTCTCCACTGGATGGAGGAAAACCAAGCCGTG GCTTCCCGCTCAGCCCTCCCTGCCCCTCCCTTCAACCATTCCCCATGGGAAATGTCAACAAGTATGAATAAAGACACCTACTGAGTGGCCGTGTTTGCCA TCTGTTTTAGCAGAGCCTAGACAAGGGCCACAGACCCAGCCAGAAGCGGAAACTTAAAAAGTCCGAATCTCTGCTCCCTGCAGGGCACAGGTGATGGTGT CTGCTGGAAAGGTCAGAGCTTCCAAAGTAAACAGCAAGAGAACCTCAGGGAGAGTAAGCTCTAGTCCCTCTGTCCTGTAGAAAGAGCCCTGAAGAATCAG CAATTTTGTTGCTTTATTGTGGCATCTGTTCGAGGTTTGCTTCCTCTTTAAGTCTGTTTCTTCATTAGCAATCATATCAGTTTTAATGCTACTACTAACA ATGAACAGTAACAATAATATCCCCCTCAATTAATAGAGTGCTTTCTATGTGCAAGGCACTTTTCACGTGTCACCTATTTTAACCTTTCCAACCACATAAA TAAAAAAGGCCATTATTAGTTGAATCTTATTGATGAAGAGA

MMP2.u.f.4 (forward primer)
MMP2.u.r.3 (reverse primer)
Intron retention

Frame 0

WGDEVVH*GWT*RPGVAGGRGKEVEKGKKRRKV EEGE*GGGAERGGPECAPRPQPRSASSLPAQPAT SGGCPPLFPLHPDFLRRWLEAAHLGL*TYKGIA RTCGGGGGGGGWGAGAGP*AAEPGKPQATEPAD PRSAALRRGAGSNQAARRPHAPSQRPPGDARGQG ALRWRR*WPGARSRVP*GRSVSWAAC*ATPPPR RRPSSSSPAMSPPKRTKSWQCNT*TPSMAAPRR AATCLC*RTH*RRCRSSLDCPRQVILTRIPSRPC GSHAAATQMWPTTTSSLASPSGTRTRSHTGSLA THLIWTQRQWMMPLLVPSKSGAM*PHCGFLESM MERQTS*STLAAGSMAMDTPLTVRTDSWLMPSPQ ALVLGETPILMTMSYGPWEKAKWSV*SMGTPMG STASSPSCSMARSTTAALIPAAAMASSGAPPPT TLRRMASTASVPMKPCSPWAATLKDSPASFHSAS RAHPMTAAPLRAARMATAGAAPLRTTTATRSMA SALRPPCPLLVGTQKVPPVSSPSLSWATNMRAA PAPAAVTERCGVRPQPTTMMTASGASALTKGTAC SSWQPTSLATPWGWSTPKTLGP*WHPFTPTPRT SVCPRMTSRAFRSSMGPLLTLTLAPAPPPRWAL SLLRSANRTLYLMASLRSVVRSSSSRTGSFGGL* RHVTSPWGPCWWPHSGLSSRKRLMRYTRPHRRR RLCSLQGMNTGSTQPAPWSEGTPSH*PAWDCPL MSSEWMPPLTGAKTRRHTSLLETNSGDTMR*RRK WILASPSSSQMPGMPSPITWMPSWTCRAAVTAT SSRVPIT*SWRTKV*RA*SLEASNPTG*AAELA LAPTGPSSPLPSIHRAWRTREGPGGAWQPCLQLY S*SAFSLLPGNLRFQRVAPPGAQE*MLTVLLPG APSPSNPTNPQSHP*RDTLIFSTQPCFGLPWCC HTSGSSPFTTFCGSQNPWSQWRLSQEGTGGPTAW HRAVGQGMARWPLQTPGFSLLAALEPFLH*QFA LYALCFFLWVLFFFST*KLHFLTEGLRLSEVTA QCISAHIVMVPLFTLLSMSLPSLFSTGWRKTKPW LPAQPSLPLPSTIPHGKCQQV*IKTPTEWPCLP SVLAEPRQGPQTQPEAET*KVRISAPCRAQVMV SAGKVRASKVNSKRTSGRVSSSPSVL*KEP*RIS NFVALLWHLFEVCFLFKSVSSLAIISVLMLLLT MNSNNNIPLN**SAFYVQGTFHVSPILTFPTT* IKKAIIS*ILLMKR

Frame 1

GVTRSCTEGGRRGQE*QAAGEKRWRKEKRGEKW RRASRGVGQRGAGPSAPPAPSPALPAPSQPSRL HLAAALPCFRCIQTSSGGGWRLRIWGFKHTKGLP GPAAAAAAAGAGARGPDHEPLSRANPRPPSQRT LGAQPCAAEQAPTRRRGGHTHRASDPRATRGAR ERYDGGANGPGRAHGSPEGALSPGLPAEPRRRRA VAHHQVPRRCRPQNGQRVGSAIPEHLLWLPQGE LQPVCAEGHTKEDAEVLWTAPDR*S*PEYHRDH AEATLRQPRCGQLQLLPSQAQVGQEPDHIQDHWL HT*SGPRDSG*CLCSCLPSLERCDPTAVFSNP* WRGRHHDQLWPLGAWRWIPL*R*GRTPGSCLRP RHWCWGRLPF**R*AMDLGRRPSGPCEVWERRWG VLQVPLLVQWQGVQQLH*YRPQRWLPLVLHHLQ L*EGWQVRLLSP*SPVHHGRQR*RTALQVSIPL PGHIL*QLHH*GPHGWLPLVRHH*GLRPRQEVWL LP*DRHVHCWWELRRCPLCLPLHFPGQQI*ELH QRRPQ*RKDVVCDHSQLR**PQVGLLP*PRVQP VPRGSPRVWPRHGAGALPRPWGPDGTHLHLHQEL PSVPG*HQGHSGALWGLS*H*PWHRPHPHAGPC HS*DLQTGHCI*WHRSDPW*DLLLQGPVHLADC DAT*QAHGAPAGGHILA*APGKD*CGIRGPTGGE GCVLCRE*ILDLLSQHPGARVPQATDQPGTAP* CPASGCRL*LEQKQEDIHLCWRQILEIQ*GEEE NGSWLPQAHRRCLECHPR*PGCRRGPAGRRSQLL LQGCLLPEAGEPKSEEREVWKHQIRLARLLSWP WLPQALPLHCLRYTGPGELEKDPEGPGSRAFSS TANQHSHSYLVI*DSREWLLPVPKNRC*LYSSQA PLPPPIPPTLRATPKEIL*YFQRSPALGCPGAA TLQALLLSQPSVAHRTLGANGDCLKRALVARQP GTGQWDRAWPGGHSRPLAFHCWLP*NLSYISSLL CMHFVFFFGSCFFFPLRNCIS*QKDSGCLKSLH SASQPT**WFPCSLYLACPYRVSSPLDGGKPSR GFPLSPPCPSLQPFPMGNVNKYE*RHLLSGRVCH LF*QSLDKGHRPSQKRKLKKSESLLPAGHR*WC LLERSELPK*TAREPQGE*ALVPLSCRKSPEES AILLLYCGICSRFASSLSLFLH*QSYQF*CYY*Q *TVTIISPSINRVLSMCKALFTCHLF*PFQPHK *KRPLLVESY**R

Frame 2

G*RGRALRVDVEARSSRRPGKRGGERKKEEKSG GGRVGGWGREGRARVRPPPPAPLCQLPPSPAGY IWRLPSLVSAASRLPQAVAGGCASGALNIQRDC QDLRRRRRRRGLGRGGRTMSR*AGQTPGHRASGP SERSPAPRSRLQPGGEAATRTEPATPGRRAGPG SATMEALMARGALTGPLRALCLLGCLLSHAAAA PSPIIKFPGDVAPKTDKELAVQYLNTFYGCPKES CNLFVLKDTLKKMQKFFGLPQTGDLDQNTIETM RKPRCGNPDVANYNFFPRKPKWDKNQITYRIIG YTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHD GEADIMINFGRWEHGDGYPFDGKDGLLAHAFAP GTGVGGDSHFDDDELWTLGEGQVVRVKYGNADG EYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYN FEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRF QGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYG FCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCT SAGRSDGKMWCATTANYDDDRKWGFCPDQGYSL FLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKN FRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPV TPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTV TPRDKPMGPLLVATFWPELPEKIDAVYEAPQEE KAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPD VQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKK MDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSY FFKGAYYLKLENQSLKSVKFGSIKSDWLGC*AGP GSHRPFLSTAFDTPGLEN*RRTRRGLAAVPSAL QLISILTPTW*FKIPESGSSRCPRIDADCTPPR RPFPLQSHQPSEPPLKRYFDIFNAALLWAALVLP HFRLFSFHNLLWLTEPLEPMETVSRGHWWPDSL AQGSGTGHGQVATPDPWLFTAGCLRTFLTLAVC FVCTLFFSLGLVFFFHLEIAFPDRRTQVV*SHCT VHLSPHSDGSPVHST*HVPTESLLHWMEENQAV ASRSALPAPPFNHSPWEMSTSMNKDTY*VAVFA ICFSRA*TRATDPARSGNLKSPNLCSLQGTGDGV CWKGQSFQSKQQENLRESKL*SLCPVERALKNQ QFCCFIVASVRGLLPL*VCFFISNHISFNATTN NEQ*Q*YPPQLIECFLCARHFSRVTYFNLSNHIN KKGHY*LNLIDEE