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Sequences

Sequence HMGA1.sAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCG
GCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAG
CTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGC
GAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAAC
CAAGGGGCAGACCCAAAAAACTGGTAGGTGAAGAAGCAGACTGCTGCTTGCCTCCTGGGCTCTTTTGAGTTGAGGGTGTTGAGTACACAGTACCTGATGC
TATGCACCCCCTATGGAAAGGCTCTCCTTGACCTGCTGGGACATCAGATTTTACAGAAGTCCAGAGAGGGGAAGGTACCTGGCCTGGGCTGTGCCCATGA
GAAGTGAGGGGTCCCAGGTATAAATCAGACCACATCCCCCTGCCCTGCCCTGCCCTAGTTGTGTGTGGGGGTCCCTCCCTCTCCTGCTCCTAGAATACTC
AGAACTTCTAGGGGAGATCTTGGAAGTCATCTAGCCTGTGTCCCCCTCAATTAGAGATGAGGAAAGGAAGCCATAGGGGGAAGGTTTGTCCTTCCTATGA
GCCTCTGCAGAAGAGAAACAGCGAAGGAGCTGGGCCCTGGGAGGGGTCGGTGCTGGAGTTCTGATGTGACCCACCACACTGCACTGGAGGGCACCATCCA
ATTCTGGGTCCCCAAACAGCTGGTGGAAAGGCTCGGTGGGCTGAGTCAAGAAGCTGCCTCTAGGGGGCCACTGCAGTTAGGGTCACC
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRSAFATSGGRGGARPVLSAQHRRSR
QPGARTAGRRPSSRIPAITLPPAP*RREDE*VE
LEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKET
KGQTQKTGR*RSRLLLASWALLS*GC*VHST*C
YAPPMERLSLTCWDIRFYRSPERGRYLAWAVPM
RSEGSQV*IRPHPPALPCPSCVWGSLPLLLLEYS
ELLGEILEVI*PVSPSIRDEERKP*GEGLSFL*
ASAEEKQRRSWALGGVGAGVLM*PTTLHWRAPS
NSGSPNSWWKGSVG*VKKLPLGGHCS*GH

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEDPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSESS
SKSSQPLASKQEKDGTEKRGRGRPRKQPPKEPS
EVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK
PRGRPKKLVGEEADCCLPPGLF*VEGVEYTVPDA
MHPLWKGSP*PAGTSDFTEVQRGEGTWPGLCP*
EVRGPRYKSDHIPLPCPALVVCGGPSLSCS*NT
QNF*GRSWKSSSLCPPQLEMRKGSHRGKVCPSYE
PLQKRNSEGAGPWEGSVLEF*CDPPHCTGGHHP
ILGPQTAGGKARWAESRSCL*GATAVRVT

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKIRICYQRRPRRSQAGPQRPAPPLP
ATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSPA
KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGN
QGADPKNW*VKKQTAACLLGSFELRVLSTQYLM
LCTPYGKALLDLLGHQILQKSREGKVPGLGCAHE
K*GVPGINQTTSPCPALP*LCVGVPPSPAPRIL
RTSRGDLGSHLACVPLN*R*GKEAIGGRFVLPM
SLCRRETAKELGPGRGRCWSSDVTHHTALEGTIQ
FWVPKQLVERLGGLSQEAASRGPLQLGS

Sequence HMGA1.eAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCG
GCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAG
CTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGG
ACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGA
CCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGA
GGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCC
CCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCA
TGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCA
CTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACT
GTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCT
GGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCC
CCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCC
CCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGA
GGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCC
GCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTT
CAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGAT
ACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGA
GTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGC
TCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRSAFATSGGRGGARPVLSAQHRRSR
QPGARTAGRRPSSRIPAITLPPAP*RREDE*VE
LEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQD
PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG
GAVTHACRLLLTGGAASFWDWTALLRSHRPHPF
PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH
GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP
LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH
CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL
AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS
PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS
PLALRLGSEYILW*WRCSHLLSR*GPRALWPPPE
VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP
APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC
SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI
LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR
VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG
LPLWFPICSYLNKKNILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEDPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSESS
SKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPG
TALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK
TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE
EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS
PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP
MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH
LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT
VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP
WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP
QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA
PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL
RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP
LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV
QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR
YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE
*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG
SLSGFLFAVT*IKKISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKIRICYQRRPRRSQAGPQRPAPPLP
ATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VPG
QRW*GVRRSPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR
RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP
QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP
WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP
T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL
FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP
GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF
PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP
PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*
GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA
RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF
NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD
TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ
SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA
PSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.qAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGA
GGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCT
TCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTC
CCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATC
CCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTC
ACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGC
CCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCT
TCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACA
GCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACC
TGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTG
CCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTT
GTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAA
GATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGC
AGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGG
GGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESSE
EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP
SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA
PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS
HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF
TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL
PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL
PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT
APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH
LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC
PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF
VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP
RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA
E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW
GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVRRRKRRASRRSPR
RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL
PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL
PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H
PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS
LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC
PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS
FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ
PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT
*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP
ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL
FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ
DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ
QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG
APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQG*EGGRGGHLAGVLG
GGAVTHACRLLLTGGAASFWDWTALLRSHRPHP
FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS
HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI
PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL
HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA
LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP
SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH
SPLALRLGSEYILW*WRCSHLLSR*GPRALWPPP
EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL
PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF
CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK
ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS
RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL
GLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.oAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGA
AGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAG
CTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACA
CTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATAC
ACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTT
TTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCC
ATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTT
TGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGA
GTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAG
GTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGA
TGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACA
AACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCC
TCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAA
GGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGG
GAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQEPSEVPTPKRPRGRPKGSKNKGEKEE
EEGISQESSEEEQ*PMRAACSSLEEQLPSGTGQ
LCSAPTAPTPSPGPPSPPPLAATPIFHLCPHHH
TTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTHT
HMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGL
LVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPKP
HLILARPTPLPWQQQVWPMEGGAGPQDSPSQTVF
VTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG*
VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYCP
GEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSSD
GAPITRSSPCPLPTPLLLLPAPQGSGSIFPLFT
NYLWTVVLFFVQCSILRHPSLLLLPAPNVHPHC
LLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGK
VGPCP*DAAAE*ARGPKSTIKGVGATSSPCSVG
EG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRSPAKCQHLRDLGADQREAKTRVRRR
KRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLDS
FAPLPPPPPLPQAHHHHRLWPPPPSSTCALTTT
LHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPRI
HTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLGF
WFGGALSAPSLFPLASHSGAWEGSPGLKRGPSP
ISSWHALLHCPGSSRCGQWRGVLAPRIPPAKLS
LSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGE
SPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQ
VRPKSPVAAT*GGLGLLP*PYFASATQPFPPPQ
MGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQ
TTSGQLCCFLFNVPFFDIRHCCCYQRQMFILIA
SCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQG
RWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLG
RGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESGAQRSANT*ETSGPTKGKQKQG*EGG
RGGHLAGVLGGGAVTHACRLLLTGGAASFWDWT
ALLRSHRPHPFPRPTITTASGRHPHLPPVPSPPH
YTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAY
THALLDKANIPLSRTLHLLRPHSLGGGDIALWA
FGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQAP
SHPGTPYSTALAAAGVANGGGCWPPGFPQPNCL
CHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGGV
SPLLPLHCGHSPLALRLGSEYILW*WRCSHLLSR
*GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLR
WGTNNKELTLPAPNPPPAPPCPPRFWFHFSSVH
KLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSLP
PVLPTIPSPKILFVGKRGWGMAGWVTDYPSPRE
GGALPLGCCSRVSKGAQIDHKGCRGHLLPLFCW
GGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.mAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGA
GGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGA
CAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACC
ACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCA
TACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGG
CTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGC
CCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGT
CTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGG
TGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTC
CAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTC
AGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTC
ACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATT
GCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGG
GAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTT
GGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGEKE
EEEGISQESSEEEQ*PMRAACSSLEEQLPSGTG
QLCSAPTAPTPSPGPPSPPPLAATPIFHLCPHH
HTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTH
THMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSG
LLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPK
PHLILARPTPLPWQQQVWPMEGGAGPQDSPSQTV
FVTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG
*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYC
PGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSS
DGAPITRSSPCPLPTPLLLLPAPQGSGSIFPLF
TNYLWTVVLFFVQCSILRHPSLLLLPAPNVHPH
CLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPG
KVGPCP*DAAAE*ARGPKSTIKGVGATSSPCSV
GEG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRRSPAKCQHLRDLGADQREAKTRVRR
RKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLD
SFAPLPPPPPLPQAHHHHRLWPPPPSSTCALTT
TLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPR
IHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLG
FWFGGALSAPSLFPLASHSGAWEGSPGLKRGPS
PISSWHALLHCPGSSRCGQWRGVLAPRIPPAKL
SLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRG
ESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIV
QVRPKSPVAAT*GGLGLLP*PYFASATQPFPPP
QMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCS
QTTSGQLCCFLFNVPFFDIRHCCCYQRQMFILI
ASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQ
GRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLL
GRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESEGAQRSANT*ETSGPTKGKQKQG*EG
GRGGHLAGVLGGGAVTHACRLLLTGGAASFWDW
TALLRSHRPHPFPRPTITTASGRHPHLPPVPSPP
HYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHA
YTHALLDKANIPLSRTLHLLRPHSLGGGDIALW
AFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQA
PSHPGTPYSTALAAAGVANGGGCWPPGFPQPNC
LCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGG
VSPLLPLHCGHSPLALRLGSEYILW*WRCSHLLS
R*GPRALWPPPEVGWGCSPNPTLLPPLSHFPLL
RWGTNNKELTLPAPNPPPAPPCPPRFWFHFSSV
HKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSL
PPVLPTIPSPKILFVGKRGWGMAGWVTDYPSPR
EGGALPLGCCSRVSKGAQIDHKGCRGHLLPLFC
WGGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.lAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCG
AAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACC
AAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCA
CTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCA
CCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTC
AGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACT
CCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAG
GGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGC
TGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAG
CCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATT
GTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTG
CTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCC
CAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGC
TGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCA
GGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGT
GTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAA
TAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPEPS
EVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKP
RGRPKKLEKEEEEGISQESSEEEQ*PMRAACSS
LEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLAA
TPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLAS
VPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVPT
PLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGLG
GFPWP*KGPKPHLILARPTPLPWQQQVWPMEGGA
GPQDSPSQTVFVTTWGSLFILPQLP*SPY*VGQ
PPSVTGRQEG*VPYSLFTVATAPLPSAWDLSTY
CGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLLC
FRHSAISPSSDGAPITRSSPCPLPTPLLLLPAP
QGSGSIFPLFTNYLWTVVLFFVQCSILRHPSLL
LLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAWQ
AG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKG
VGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*
IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRSPA
KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGN
QGADPKNWRRRKRRASRRSPRRRSSDPCVPPAPH
WRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWPP
PPSSTCALTTTLHSTPAAAGLPWAEWGAVFPWP
QFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASPL
PWWWGHCSLGFWFGGALSAPSLFPLASHSGAWE
GSPGLKRGPSPISSWHALLHCPGSSRCGQWRGV
LAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLDS
PLRLQEGRRGESPTPSSLWPQPPCPPPGI*VHI
VVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PYF
ASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLPP
KVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHCC
CYQRQMFILIASCSAHDPLPQDTLCGEEGLGHG
RLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV
*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE
*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGAQR
SANT*ETSGPTKGKQKQGCCQDPENHHNSRKET
KGQTQKTGEGGRGGHLAGVLGGGAVTHACRLLL
TGGAASFWDWTALLRSHRPHPFPRPTITTASGRH
PHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPGL
SSQLPPPTHAYTHALLDKANIPLSRTLHLLRPH
SLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPGR
VPLALKGAQAPSHPGTPYSTALAAAGVANGGGC
WPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGWT
APFGYRKAGGVSPLLPLHCGHSPLALRLGSEYIL
W*WRCSHLLSR*GPRALWPPPEVGWGCSPNPTL
LPPLSHFPLLRWGTNNKELTLPAPNPPPAPPCP
PRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIAA
ATSAKCSSSLPPVLPTIPSPKILFVGKRGWGMA
GWVTDYPSPREGGALPLGCCSRVSKGAQIDHKG
CRGHLLPLFCWGGVAMICPSLGLPLWFPICSYLN
KKNILFWTG

Sequence HMGA1.jAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA
ACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC
TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATG
GAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCA
CTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCT
GGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAG
CGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTG
ACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCC
ACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAA
TATCCTTTTCTGGACTGGAG
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK
PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS
SLEEQLPSGTGQLCSAPTAPLPSAWDLSTYCGD
GDAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH
SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGS
GSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP
APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*
PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGA
TSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK
ISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP
HWRSSFLLGLDSFAPLPQPPCPPPGI*VHIVVM
EMQSLIVQVRPKSPVAAT*GGLGLLP*PYFASA
TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL
VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQ
RQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG
DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP
PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KK
YPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE
TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHSPLALRLGSEYILW*W
RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPP
LSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF
WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS
AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWV
TDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG
HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN
ILFWTG

Sequence HMGA1.iAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA
ACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC
TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCG
CCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGC
CTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCC
ACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGG
GAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGG
TGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGA
CAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACAT
ATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACT
TTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCC
CCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGC
TGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATG
GCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAG
GGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTT
GAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK
PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS
SLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA
ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLA
SVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVP
TPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL
GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEGG
AGPQDSPSQTVFVTTWGSLFILPQLP*SPY*VG
QPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST
YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLL
CFRHSAISPSSDGAPITRSSPCPLPTPLLLLPA
PQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL
LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAW
QAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIK
GVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT
*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP
HWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWP
PPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW
PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASP
LPWWWGHCSLGFWFGGALSAPSLFPLASHSGAW
EGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG
VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLD
SPLRLQEGRRGESPTPSSLWPQPPCPPPGI*VH
IVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY
FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLP
PKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHC
CCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH
GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*R
V*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLL
E*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE
TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHRPHPFPRPTITTASGR
HPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPG
LSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP
HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPG
RVPLALKGAQAPSHPGTPYSTALAAAGVANGGG
CWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW
TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEYI
LW*WRCSHLLSR*GPRALWPPPEVGWGCSPNPT
LLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC
PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIA
AATSAKCSSSLPPVLPTIPSPKILFVGKRGWGM
AGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK
GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSYL
NKKNILFWTG

Sequence HMGA1.gAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGAC
CCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAG
GAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCC
CAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCAT
GGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCAC
TTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTG
TTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTG
GCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCC
CAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCC
CCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAG
GTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCG
CTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTC
AATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATA
CTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAG
TGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCT
CCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQEPSEVPTPKRPRGRPKGSKNKGAAKT
RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE
EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS
PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM
G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH
LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT
VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW
QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP
QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA
PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR
WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP
LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV
QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY
SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE
*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG
SLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRSPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR
SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP
QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP
WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT
*PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL
FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP
GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP
NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP
PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*
GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR
SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF
NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD
TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS
EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA
PSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESGAQRSANT*ETSGPTKGKQKQGCCQD
PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG
GAVTHACRLLLTGGAASFWDWTALLRSHRPHPFP
RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH
GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP
LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC
SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL
AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS
PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP
LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE
VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP
APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS
MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI
LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR
VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL
PLWFPICSYLNKKNILFWTG

Sequence HMGA1.fAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAA
GACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAG
GAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTT
CCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCC
CATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCC
CACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCA
CTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCC
CTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTT
CCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAG
CCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCT
GAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGC
CCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTG
TTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAG
ATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCA
GAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGG
GCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK
TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSE
EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP
SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP
MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS
HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF
TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP
WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL
PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT
APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL
RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC
PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF
VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR
YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA
E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW
GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRRSPAKCQHLRDLGADQREAKTRVLP
RPGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR
RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL
PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL
PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP
T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS
LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC
PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF
PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ
PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT
*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA
RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL
FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ
DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ
SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG
APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQ
DPENHHNSRKETKGQTQKTGEGGRGGHLAGVLG
GGAVTHACRLLLTGGAASFWDWTALLRSHRPHPF
PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS
HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI
PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH
CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA
LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP
SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS
PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP
EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL
PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC
SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK
ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS
RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG
LPLWFPICSYLNKKNILFWTG

Sequence HMGA1.bAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGAC
CCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGCTCTCCTTGACCTGCTGGGACATCAGATTTTACAGAAGTCCAGA
GAGGGGAAGGTACCTGGCCTGGGCTGTGCCCATGAGAAGTGAGGGGTCCCAGGTATAAATCAGACCACATCCCCCTGCCCTGCCCTGCCCTAGTTGTGTG
TGGGGGTCCCTCCCTCTCCTGCTCCTAGAATACTCAGAACTTCTAGGGGAGATCTTGGAAGTCATCTAGCCTGTGTCCCCCTCAATTAGAGATGAGGAAA
GGAAGCCATAGGGGGAAGGTTTGTCCTTCCTATGAGCCTCTGCAGAAGAGAAACAGCGAAGGAGCTGGGCCCTGGGAGGGGTCGGTGCTGGAGTTCTGAT
GTGACCCACCACACTGCACTGGAGGGCACCATCCAATTCTGGGTCCCCAAACAGCTGGTGGAAAGGCTCGGTGGGCTGAGTCAAGAAGCTGCCTCTAGGG
GGCCACTGCAGTTAGGGTCACC
  • HMGA1.u.f.5 (forward primer)
  • HMGA1.u.r.9 (reverse primer)
  • Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQEPSEVPTPKRPRGRPKGSKNKGAAKT
RKTTTTPGRKPRGRPKKLALLDLLGHQILQKSR
EGKVPGLGCAHEK*GVPGINQTTSPCPALP*LC
VGVPPSPAPRILRTSRGDLGSHLACVPLN*R*GK
EAIGGRFVLPMSLCRRETAKELGPGRGRCWSSD
VTHHTALEGTIQFWVPKQLVERLGGLSQEAASR
GPLQLGS

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRSPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNWLSLTCWDIRFYRSPE
RGRYLAWAVPMRSEGSQV*IRPHPPALPCPSCV
WGSLPLLLLEYSELLGEILEVI*PVSPSIRDEE
RKP*GEGLSFL*ASAEEKQRRSWALGGVGAGVLM
*PTTLHWRAPSNSGSPNSWWKGSVG*VKKLPLG
GHCS*GH

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESGAQRSANT*ETSGPTKGKQKQGCCQD
PENHHNSRKETKGQTQKTGSP*PAGTSDFTEVQ
RGEGTWPGLCP*EVRGPRYKSDHIPLPCPALVVC
GGPSLSCS*NTQNF*GRSWKSSSLCPPQLEMRK
GSHRGKVCPSYEPLQKRNSEGAGPWEGSVLEF*
CDPPHCTGGHHPILGPQTAGGKARWAESRSCL*G
ATAVRVT

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