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Sequences

Sequence HMGA1.vcAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCCATCCCAGCCATCACTCTTCCACCTGCTCCTT AGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGC GCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGG AAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAG GGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCT CCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACA GCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATG CCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTT GGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCAT CCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACC ACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCT ACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGC CCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCA CCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACC TCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTT CTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGG CCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGT AGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAAIPAITLPPAP *RREDE*VELEVQPALGLQAGKGRH*EAGPGQAA QAASGESRDSAGRESEGAQRSANT*ETSGPTKG KQKQGCCQDPENHHNSRKETKGQTQKTGEGGRG GHLAGVLGGGAVTHACRLLLTGGAASFWDWTALL RSHRPHPFPRPTITTASGRHPHLPPVPSPPHYT AHQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTH ALLDKANIPLSRTLHLLRPHSLGGGDIALWAFGL GAPSLLLHCSLWLPIVGPGRVPLALKGAQAPSH PGTPYSTALAAAGVANGGGCWPPGFPQPNCLCH HVGLTFHPSPTSLVPVLGWTAPFGYRKAGGVSPL LPLHCGHSPLALRLGSEYILW*WRCSHLLSR*G PRALWPPPEVGWGCSPNPTLLPPLSHFPLLRWG TNNKELTLPAPNPPPAPPCPPRFWFHFSSVHKLP LDSCVVFCSMFHSSTSVIAAATSAKCSSSLPPV LPTIPSPKILFVGKRGWGMAGWVTDYPSPREGG ALPLGCCSRVSKGAQIDHKGCRGHLLPLFCWGGV AMICPSLGLPLWFPICSYLNKKNILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLPSQPSLFHLLL REGKMSESSSKSSQPLASKQEKDGTEKRGRGRP RKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKG SKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEE GISQESSEEEQ*PMRAACSSLEEQLPSGTGQLC SAPTAPTPSPGPPSPPPLAATPIFHLCPHHHTTQ HTSRCRAPMG*VGSSFPLASVPSSPRPPTHTHM PSWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLV WGRPLCSFTVPSGFP*WGLGGFPWP*KGPKPHLI LARPTPLPWQQQVWPMEGGAGPQDSPSQTVFVT TWGSLFILPQLP*SPY*VGQPPSVTGRQEG*VP YSLFTVATAPLPSAWDLSTYCGDGDAVTYCPGEA QEPCGRHLRWAGAAPLTLLCFRHSAISPSSDGA PITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNY LWTVVLFFVQCSILRHPSLLLLPAPNVHPHCLLF CPRSPPPRYSLWGRGAGAWQAG*PTTPVPGKVG PCP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG *P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCHPSHHSSTCSL EKGR*VSRARSPASPWPPSRKRTALRSGAGAGR ASSLR*VPGQRW*GVRRSPAKCQHLRDLGADQR EAKTRVLPRPGKPPQLQEGNQGADPKNWRRRKRR ASRRSPRRRSSDPCVPPAPHWRSSFLLGLDSFA PLPPPPPLPQAHHHHRLWPPPPSSTCALTTTLH STPAAAGLPWAEWGAVFPWPQFPAPPAHPRIHTC PPGQG*HPT*PHPAPAASPLPWWWGHCSLGFWF GGALSAPSLFPLASHSGAWEGSPGLKRGPSPIS SWHALLHCPGSSRCGQWRGVLAPRIPPAKLSLSP RGAHFSSFPNFPSPRTRLDSPLRLQEGRRGESP TPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVR PKSPVAAT*GGLGLLP*PYFASATQPFPPPQMGH Q*QGAHPARSQPPSCSSLPPKVLVPFFLCSQTT SGQLCCFLFNVPFFDIRHCCCYQRQMFILIASC SAHDPLPQDTLCGEEGLGHGRLGDRLPQSQGRWG PAPRMLQQSEQGGPNRP*RV*GPPPPPVLLGRG SHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.sAug05

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRSAFATSGGRGGARPVLSAQHRRSR QPGARTAGRRPSSRIPAITLPPAP*RREDE*VE LEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKET KGQTQKTGR*RSRLLLASWALLS*GC*VHST*C YAPPMERLSLTCWDIRFYRSPERGRYLAWAVPM RSEGSQV*IRPHPPALPCPSCVWGSLPLLLLEYS ELLGEILEVI*PVSPSIRDEERKP*GEGLSFL* ASAEEKQRRSWALGGVGAGVLM*PTTLHWRAPS NSGSPNSWWKGSVG*VKKLPLGGHCS*GH

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEDPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSESS SKSSQPLASKQEKDGTEKRGRGRPRKQPPKEPS EVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK PRGRPKKLVGEEADCCLPPGLF*VEGVEYTVPDA MHPLWKGSP*PAGTSDFTEVQRGEGTWPGLCP* EVRGPRYKSDHIPLPCPALVVCGGPSLSCS*NT QNF*GRSWKSSSLCPPQLEMRKGSHRGKVCPSYE PLQKRNSEGAGPWEGSVLEF*CDPPHCTGGHHP ILGPQTAGGKARWAESRSCL*GATAVRVT

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKIRICYQRRPRRSQAGPQRPAPPLP ATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSPA KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGN QGADPKNW*VKKQTAACLLGSFELRVLSTQYLM LCTPYGKALLDLLGHQILQKSREGKVPGLGCAHE K*GVPGINQTTSPCPALP*LCVGVPPSPAPRIL RTSRGDLGSHLACVPLN*R*GKEAIGGRFVLPM SLCRRETAKELGPGRGRCWSSDVTHHTALEGTIQ FWVPKQLVERLGGLSQEAASRGPLQLGS

Sequence HMGA1.eAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCG GCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAG CTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGG ACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGA CCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGA GGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCC CCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCA TGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCA CTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACT GTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCT GGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCC CCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCC CCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGA GGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCC GCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTT CAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGAT ACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGA GTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGC TCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRSAFATSGGRGGARPVLSAQHRRSR QPGARTAGRRPSSRIPAITLPPAP*RREDE*VE LEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQD PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG GAVTHACRLLLTGGAASFWDWTALLRSHRPHPF PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS PLALRLGSEYILW*WRCSHLLSR*GPRALWPPPE VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG LPLWFPICSYLNKKNILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEDPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSESS SKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPG TALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE *ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG SLSGFLFAVT*IKKISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKIRICYQRRPRRSQAGPQRPAPPLP ATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VPG QRW*GVRRSPAKCQHLRDLGADQREAKTRVLPR PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT* GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA PSLVSYLQLLE*KKYPFLDW