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Sequences

Sequence HMGA1.vgAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCC AGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACC TAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCC AAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGC TTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCA CCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCC CCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGG GGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCC TTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGAT TCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTA CAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGA TGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCA GCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTT CCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCC AAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCG ACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCT CCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATC CTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRSIPAITLPPAP*RREDE*VELEVQP ALGLQAGKGRH*EAGPGQAAQAASEGAQRSANT *ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGAA SFWDWTALLRSHRPHPFPRPTITTASGRHPHLP PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGGG DIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFGY RKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFWF HFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGHL LPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI LFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEASQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPTP KRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIFH LCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPWP *KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDGD AVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPAP NVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKIS FSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKHPSHHSSTCSLEKGR*VSRARSPA SPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH LRDLGADQREAKTRVLPRPGKPPQLQEGNQGADP KNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPAP PAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPRI PPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASATQ PFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGDR LPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY PFLDW

Sequence HMGA1.vmAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA GGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCA TCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGC TCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCAC ACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCT CCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGG GCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTG GCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGT GGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTC CCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAA GAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAA TAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTG GACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGC CCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCT GCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCC ATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQKEPSEVPTPKRPRGRPKGSKNKGEKEEEEG ISQESSEEEQ*PMRAACSSLEEQLPSGTGQLCSA PTAPTPSPGPPSPPPLAATPIFHLCPHHHTTQH TSRCRAPMG*VGSSFPLASVPSSPRPPTHTHMP SWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLVWG RPLCSFTVPSGFP*WGLGGFPWP*KGPKPHLIL ARPTPLPWQQQVWPMEGGAGPQDSPSQTVFVTT WGSLFILPQLP*SPY*VGQPPSVTGRQEG*VPYS LFTVATAPLPSAWDLSTYCGDGDAVTYCPGEAQ EPCGRHLRWAGAAPLTLLCFRHSAISPSSDGAP ITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNYLW TVVLFFVQCSILRHPSLLLLPAPNVHPHCLLFC PRSPPPRYSLWGRGAGAWQAG*PTTPVPGKVGP CP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG*P *FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRRSPAKCQHLRDLGADQREAKTRVRRRKRRA SRRSPRRRSSDPCVPPAPHWRSSFLLGLDSFAP LPPPPPLPQAHHHHRLWPPPPSSTCALTTTLHST PAAAGLPWAEWGAVFPWPQFPAPPAHPRIHTCP PGQG*HPT*PHPAPAASPLPWWWGHCSLGFWFG GALSAPSLFPLASHSGAWEGSPGLKRGPSPISSW HALLHCPGSSRCGQWRGVLAPRIPPAKLSLSPR GAHFSSFPNFPSPRTRLDSPLRLQEGRRGESPT PSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVRPK SPVAAT*GGLGLLP*PYFASATQPFPPPQMGHQ *QGAHPARSQPPSCSSLPPKVLVPFFLCSQTTS GQLCCFLFNVPFFDIRHCCCYQRQMFILIASCSA HDPLPQDTLCGEEGLGHGRLGDRLPQSQGRWGP APRMLQQSEQGGPNRP*RV*GPPPPPVLLGRGS HDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESEGAQRSANT*ETSGPTKGKQKQG*EGGRGGH LAGVLGGGAVTHACRLLLTGGAASFWDWTALLR SHRPHPFPRPTITTASGRHPHLPPVPSPPHYTA HQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTHAL LDKANIPLSRTLHLLRPHSLGGGDIALWAFGLG APSLLLHCSLWLPIVGPGRVPLALKGAQAPSHP GTPYSTALAAAGVANGGGCWPPGFPQPNCLCHHV GLTFHPSPTSLVPVLGWTAPFGYRKAGGVSPLL PLHCGHSPLALRLGSEYILW*WRCSHLLSR*GP RALWPPPEVGWGCSPNPTLLPPLSHFPLLRWGTN NKELTLPAPNPPPAPPCPPRFWFHFSSVHKLPL DSCVVFCSMFHSSTSVIAAATSAKCSSSLPPVL PTIPSPKILFVGKRGWGMAGWVTDYPSPREGGAL PLGCCSRVSKGAQIDHKGCRGHLLPLFCWGGVA MICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.viAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACA CCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCC CATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCC CACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCA CTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCC CTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTT CCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAG CCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCT GAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGC CCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTG TTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAG ATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCA GAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGG GCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPT PKRPRGRPKGSKNKGAAKTRTTTLHSTPAAAGL PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT *GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG APSLVSYLQLLE*KKYPFLDW

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH LRDLGADQREAKTRVLPRPGPPHYTAHQPLQGS HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG LPLWFPICSYLNKKNILFWTG

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASEGAQRSAN T*ETSGPTKGKQKQGCCQDPDHHTTQHTSRCRAP MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW GSLSGFLFAVT*IKKISFSGLE

Sequence HMGA1.veAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCGAAGTGCCAACACCT AAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCA AAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCT TCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCAC CTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCC CCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGG GACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCT TAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATT CCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTAC AGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGAT GCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAG CCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTC CATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCA AATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGA CTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTC CTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCC TTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPEPSEVPTP KRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQL PSGTGQLCSAPTAPTPSPGPPSPPPLAATPIFH LCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVVG TLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPWP *KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSVT GRQEG*VPYSLFTVATAPLPSAWDLSTYCGDGD AVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSGS IFPLFTNYLWTVVLFFVQCSILRHPSLLLLPAP NVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGATS SPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKIS FSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLRSPAKCQHL RDLGADQREAKTRVLPRPGKPPQLQEGNQGADP KNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSST CALTTTLHSTPAAAGLPWAEWGAVFPWPQFPAP PAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPGL KRGPSPISSWHALLHCPGSSRCGQWRGVLAPRI PPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVMEM QSLIVQVRPKSPVAAT*GGLGLLP*PYFASATQ PFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQRQ MFILIASCSAHDPLPQDTLCGEEGLGHGRLGDR LPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKYP FLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGAQRSANT *ETSGPTKGKQKQGCCQDPENHHNSRKETKGQTQ KTGEGGRGGHLAGVLGGGAVTHACRLLLTGGAA SFWDWTALLRSHRPHPFPRPTITTASGRHPHLP PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQLP PPTHAYTHALLDKANIPLSRTLHLLRPHSLGGG DIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPGF PQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFGY RKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPLS HFPLLRWGTNNKELTLPAPNPPPAPPCPPRFWF HFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVTD YPSPREGGALPLGCCSRVSKGAQIDHKGCRGHL LPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI LFWTG

Sequence HMGA1.vdAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACA CCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGAC CCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCA GCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTC CACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCT CCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGT GGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGG CCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGG ATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGT TACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGA GATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACT CAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGG TTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCG CCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGAC CGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCAC CTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATA TCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPT PKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGR PKKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIF HLCPHHHTTQHTSRCRAPMG*VGSSFPLASVPS SPRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPW P*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQ DSPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDG DAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPA PNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG* PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKI SFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH LRDLGADQREAKTRVLPRPGKPPQLQEGNQGAD PKNWRRRKRRASRRSPRRRSSDPCVPPAPHWRS SFLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPA PPAHPRIHTCPPGQG*HPT*PHPAPAASPLPWW WGHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPR IPPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLR LQEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASAT QPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGD RLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY PFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASEGAQRSAN T*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGA ASFWDWTALLRSHRPHPFPRPTITTASGRHPHL PPVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGG GDIALWAFGLGAPSLLLHCSLWLPIVGPGRVPL ALKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFG YRKAGGVSPLLPLHCGHSPLALRLGSEYILW*W RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFW FHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGH LLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN ILFWTG

Sequence HMGA1.vaAug05

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKT TTTPGRKPRGRPKKLVGEEADCCLPPGLF*VEGV EYTVPDAMHPLWKGSP*PAGTSDFTEVQRGEGT WPGLCP*EVRGPRYKSDHIPLPCPALVVCGGPS LSCS*NTQNF*GRSWKSSSLCPPQLEMRKGSHRG KVCPSYEPLQKRNSEGAGPWEGSVLEF*CDPPH CTGGHHPILGPQTAGGKARWAESRSCL*GATAV RVT

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRRSPAKCQHLRDLGADQREAKTRVLPRPGKP PQLQEGNQGADPKNW*VKKQTAACLLGSFELRV LSTQYLMLCTPYGKALLDLLGHQILQKSREGKVP GLGCAHEK*GVPGINQTTSPCPALP*LCVGVPP SPAPRILRTSRGDLGSHLACVPLN*R*GKEAIG GRFVLPMSLCRRETAKELGPGRGRCWSSDVTHHT ALEGTIQFWVPKQLVERLGGLSQEAASRGPLQL GS

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESEGAQRSANT*ETSGPTKGKQKQGCCQDPENH HNSRKETKGQTQKTGR*RSRLLLASWALLS*GC *VHST*CYAPPMERLSLTCWDIRFYRSPERGRY LAWAVPMRSEGSQV*IRPHPPALPCPSCVWGSLP LLLLEYSELLGEILEVI*PVSPSIRDEERKP*G EGLSFL*ASAEEKQRRSWALGGVGAGVLM*PTT LHWRAPSNSGSPNSWWKGSVG*VKKLPLGGHCS* GH

Sequence HMGA1.uAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA GGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCA CAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCAT GCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCA CCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGA GCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTG CACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTC CCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGT GGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTC CCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCC TGGGATCTGAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGC CACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTT CTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACC AGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGG TGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGG CCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAA AATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQEPSEVPTPKRPRGRPKGSKNKGAAKTRKTT TTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*PM RAACSSLEEQLPSGTGQLCSAPTAPTPSPGPPS PPPLAATPIFHLCPHHHTTQHTSRCRAPMG*VG SSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAPC TCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSGF P*WGLGGFPWP*KGPKPHLILARPTPLPWQQQV WPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP*S PY*VGQPPSVTGRQEG*VPYSLFTVATAPLPSA WDLSHLLSR*GPRALWPPPEVGWGCSPNPTLLP PLSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAAT SAKCSSSLPPVLPTIPSPKILFVGKRGWGMAGW VTDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKK NILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRSPAKCQHLRDLGADQREAKTRVLPRPGKPP QLQEGNQGADPKNWRRRKRRASRRSPRRRSSDP CVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHHH HRLWPPPPSSTCALTTTLHSTPAAAGLPWAEWG AVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PHP APAASPLPWWWGHCSLGFWFGGALSAPSLFPLAS HSGAWEGSPGLKRGPSPISSWHALLHCPGSSRC GQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFPS PRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPPP GI*VTYCPGEAQEPCGRHLRWAGAAPLTLLCFR HSAISPSSDGAPITRSSPCPLPTPLLLLPAPQG SGSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG *PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVG ATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK ISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESGAQRSANT*ETSGPTKGKQKQGCCQDPENHH NSRKETKGQTQKTGEGGRGGHLAGVLGGGAVTH ACRLLLTGGAASFWDWTALLRSHRPHPFPRPTI TTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSGE QFSPGLSSQLPPPTHAYTHALLDKANIPLSRTL HLLRPHSLGGGDIALWAFGLGAPSLLLHCSLWL PIVGPGRVPLALKGAQAPSHPGTPYSTALAAAGV ANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSLV PVLGWTAPFGYRKAGGVSPLLPLHCGHSPLALR LGSESLIVQVRPKSPVAAT*GGLGLLP*PYFASA TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKV LVPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCY QRQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*G PPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*K KYPFLDW

Sequence HMGA1.tAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA GGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCA CCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACC CATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCA TCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGG GGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACC CTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGC TTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGG TGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTA GTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCC GCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGG CTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCC CCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATT CTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGG GAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGG GCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGT TTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKT TTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*P MRAACSSLEEQLPSGTGQLCSAPTAPTPSPGPP SPPPLAATPIFHLCPHHHTTQHTSRCRAPMG*V GSSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAP CTCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSG FP*WGLGGFPWP*KGPKPHLILARPTPLPWQQQ VWPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP* SPY*VGQPPSVTGRQEG*VPYSLFTVATAPLPS AWDLSTYCGDGDAVTYCPGEAQEPCGRHLRWAG AAPLTLLCFRHSAISPSSDGAPITRSSPCPLPTP LLLLPAPQGSGSIFPLFTNYLWTVVLFFVQCSI LRHPSLLLLPAPNVHPHCLLFCPRSPPPRYSLW GRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*ARG PKSTIKGVGATSSPCSVGEG*P*FVPAWGSLSG FLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRRSPAKCQHLRDLGADQREAKTRVLPRPGKP PQLQEGNQGADPKNWRRRKRRASRRSPRRRSSD PCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHH HHRLWPPPPSSTCALTTTLHSTPAAAGLPWAEW GAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PH PAPAASPLPWWWGHCSLGFWFGGALSAPSLFPLA SHSGAWEGSPGLKRGPSPISSWHALLHCPGSSR CGQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFP SPRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPP PGI*VHIVVMEMQSLIVQVRPKSPVAAT*GGLG LLP*PYFASATQPFPPPQMGHQ*QGAHPARSQP PSCSSLPPKVLVPFFLCSQTTSGQLCCFLFNVPF FDIRHCCCYQRQMFILIASCSAHDPLPQDTLCG EEGLGHGRLGDRLPQSQGRWGPAPRMLQQSEQG GPNRP*RV*GPPPPPVLLGRGSHDLSQPGAPSLV SYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESEGAQRSANT*ETSGPTKGKQKQGCCQDPENH HNSRKETKGQTQKTGEGGRGGHLAGVLGGGAVT HACRLLLTGGAASFWDWTALLRSHRPHPFPRPT ITTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSG EQFSPGLSSQLPPPTHAYTHALLDKANIPLSRT LHLLRPHSLGGGDIALWAFGLGAPSLLLHCSLW LPIVGPGRVPLALKGAQAPSHPGTPYSTALAAAG VANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSL VPVLGWTAPFGYRKAGGVSPLLPLHCGHSPLAL RLGSEYILW*WRCSHLLSR*GPRALWPPPEVGWG CSPNPTLLPPLSHFPLLRWGTNNKELTLPAPNP PPAPPCPPRFWFHFSSVHKLPLDSCVVFCSMFH SSTSVIAAATSAKCSSSLPPVLPTIPSPKILFVG KRGWGMAGWVTDYPSPREGGALPLGCCSRVSKG AQIDHKGCRGHLLPLFCWGGVAMICPSLGLPLW FPICSYLNKKNILFWTG