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Sequences

Sequence HMGA1.vmAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA GGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCA TCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGC TCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCAC ACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCT CCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGG GCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTG GCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGT GGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTC CCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAA GAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAA TAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTG GACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGC CCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCT GCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCC ATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQKEPSEVPTPKRPRGRPKGSKNKGEKEEEEG ISQESSEEEQ*PMRAACSSLEEQLPSGTGQLCSA PTAPTPSPGPPSPPPLAATPIFHLCPHHHTTQH TSRCRAPMG*VGSSFPLASVPSSPRPPTHTHMP SWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLVWG RPLCSFTVPSGFP*WGLGGFPWP*KGPKPHLIL ARPTPLPWQQQVWPMEGGAGPQDSPSQTVFVTT WGSLFILPQLP*SPY*VGQPPSVTGRQEG*VPYS LFTVATAPLPSAWDLSTYCGDGDAVTYCPGEAQ EPCGRHLRWAGAAPLTLLCFRHSAISPSSDGAP ITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNYLW TVVLFFVQCSILRHPSLLLLPAPNVHPHCLLFC PRSPPPRYSLWGRGAGAWQAG*PTTPVPGKVGP CP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG*P *FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRRSPAKCQHLRDLGADQREAKTRVRRRKRRA SRRSPRRRSSDPCVPPAPHWRSSFLLGLDSFAP LPPPPPLPQAHHHHRLWPPPPSSTCALTTTLHST PAAAGLPWAEWGAVFPWPQFPAPPAHPRIHTCP PGQG*HPT*PHPAPAASPLPWWWGHCSLGFWFG GALSAPSLFPLASHSGAWEGSPGLKRGPSPISSW HALLHCPGSSRCGQWRGVLAPRIPPAKLSLSPR GAHFSSFPNFPSPRTRLDSPLRLQEGRRGESPT PSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVRPK SPVAAT*GGLGLLP*PYFASATQPFPPPQMGHQ *QGAHPARSQPPSCSSLPPKVLVPFFLCSQTTS GQLCCFLFNVPFFDIRHCCCYQRQMFILIASCSA HDPLPQDTLCGEEGLGHGRLGDRLPQSQGRWGP APRMLQQSEQGGPNRP*RV*GPPPPPVLLGRGS HDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESEGAQRSANT*ETSGPTKGKQKQG*EGGRGGH LAGVLGGGAVTHACRLLLTGGAASFWDWTALLR SHRPHPFPRPTITTASGRHPHLPPVPSPPHYTA HQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTHAL LDKANIPLSRTLHLLRPHSLGGGDIALWAFGLG APSLLLHCSLWLPIVGPGRVPLALKGAQAPSHP GTPYSTALAAAGVANGGGCWPPGFPQPNCLCHHV GLTFHPSPTSLVPVLGWTAPFGYRKAGGVSPLL PLHCGHSPLALRLGSEYILW*WRCSHLLSR*GP RALWPPPEVGWGCSPNPTLLPPLSHFPLLRWGTN NKELTLPAPNPPPAPPCPPRFWFHFSSVHKLPL DSCVVFCSMFHSSTSVIAAATSAKCSSSLPPVL PTIPSPKILFVGKRGWGMAGWVTDYPSPREGGAL PLGCCSRVSKGAQIDHKGCRGHLLPLFCWGGVA MICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.viAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACA CCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCC CATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCC CACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCA CTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCC CTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTT CCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAG CCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCT GAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGC CCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTG TTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAG ATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCA GAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGG GCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPT PKRPRGRPKGSKNKGAAKTRTTTLHSTPAAAGL PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT *GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG APSLVSYLQLLE*KKYPFLDW

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH LRDLGADQREAKTRVLPRPGPPHYTAHQPLQGS HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG LPLWFPICSYLNKKNILFWTG

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASEGAQRSAN T*ETSGPTKGKQKQGCCQDPDHHTTQHTSRCRAP MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW GSLSGFLFAVT*IKKISFSGLE

Sequence HMGA1.veAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCGAAGTGCCAACACCT AAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCA AAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCT TCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCAC CTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCC CCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGG GACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCT TAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATT CCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTAC AGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGAT GCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAG CCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTC CATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCA AATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGA CTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTC CTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCC TTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPEPSEVPTP KRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQL PSGTGQLCSAPTAPTPSPGPPSPPPLAATPIFH LCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVVG TLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPWP *KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSVT GRQEG*VPYSLFTVATAPLPSAWDLSTYCGDGD AVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSGS IFPLFTNYLWTVVLFFVQCSILRHPSLLLLPAP NVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGATS SPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKIS FSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLRSPAKCQHL RDLGADQREAKTRVLPRPGKPPQLQEGNQGADP KNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSST CALTTTLHSTPAAAGLPWAEWGAVFPWPQFPAP PAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPGL KRGPSPISSWHALLHCPGSSRCGQWRGVLAPRI PPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVMEM QSLIVQVRPKSPVAAT*GGLGLLP*PYFASATQ PFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQRQ MFILIASCSAHDPLPQDTLCGEEGLGHGRLGDR LPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKYP FLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGAQRSANT *ETSGPTKGKQKQGCCQDPENHHNSRKETKGQTQ KTGEGGRGGHLAGVLGGGAVTHACRLLLTGGAA SFWDWTALLRSHRPHPFPRPTITTASGRHPHLP PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQLP PPTHAYTHALLDKANIPLSRTLHLLRPHSLGGG DIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPGF PQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFGY RKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPLS HFPLLRWGTNNKELTLPAPNPPPAPPCPPRFWF HFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVTD YPSPREGGALPLGCCSRVSKGAQIDHKGCRGHL LPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI LFWTG

Sequence HMGA1.vdAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACA CCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGAC CCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCA GCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTC CACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCT CCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGT GGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGG CCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGG ATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGT TACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGA GATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACT CAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGG TTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCG CCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGAC CGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCAC CTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATA TCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPT PKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGR PKKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIF HLCPHHHTTQHTSRCRAPMG*VGSSFPLASVPS SPRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPW P*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQ DSPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDG DAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPA PNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG* PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKI SFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH LRDLGADQREAKTRVLPRPGKPPQLQEGNQGAD PKNWRRRKRRASRRSPRRRSSDPCVPPAPHWRS SFLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPA PPAHPRIHTCPPGQG*HPT*PHPAPAASPLPWW WGHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPR IPPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLR LQEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASAT QPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGD RLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY PFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASEGAQRSAN T*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGA ASFWDWTALLRSHRPHPFPRPTITTASGRHPHL PPVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGG GDIALWAFGLGAPSLLLHCSLWLPIVGPGRVPL ALKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFG YRKAGGVSPLLPLHCGHSPLALRLGSEYILW*W RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFW FHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGH LLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN ILFWTG

Sequence HMGA1.vaAug05

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKT TTTPGRKPRGRPKKLVGEEADCCLPPGLF*VEGV EYTVPDAMHPLWKGSP*PAGTSDFTEVQRGEGT WPGLCP*EVRGPRYKSDHIPLPCPALVVCGGPS LSCS*NTQNF*GRSWKSSSLCPPQLEMRKGSHRG KVCPSYEPLQKRNSEGAGPWEGSVLEF*CDPPH CTGGHHPILGPQTAGGKARWAESRSCL*GATAV RVT

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRRSPAKCQHLRDLGADQREAKTRVLPRPGKP PQLQEGNQGADPKNW*VKKQTAACLLGSFELRV LSTQYLMLCTPYGKALLDLLGHQILQKSREGKVP GLGCAHEK*GVPGINQTTSPCPALP*LCVGVPP SPAPRILRTSRGDLGSHLACVPLN*R*GKEAIG GRFVLPMSLCRRETAKELGPGRGRCWSSDVTHHT ALEGTIQFWVPKQLVERLGGLSQEAASRGPLQL GS

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESEGAQRSANT*ETSGPTKGKQKQGCCQDPENH HNSRKETKGQTQKTGR*RSRLLLASWALLS*GC *VHST*CYAPPMERLSLTCWDIRFYRSPERGRY LAWAVPMRSEGSQV*IRPHPPALPCPSCVWGSLP LLLLEYSELLGEILEVI*PVSPSIRDEERKP*G EGLSFL*ASAEEKQRRSWALGGVGAGVLM*PTT LHWRAPSNSGSPNSWWKGSVG*VKKLPLGGHCS* GH

Sequence HMGA1.uAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA GGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCA CAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCAT GCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCA CCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGA GCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTG CACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTC CCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGT GGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTC CCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCC TGGGATCTGAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGC CACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTT CTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACC AGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGG TGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGG CCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAA AATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQEPSEVPTPKRPRGRPKGSKNKGAAKTRKTT TTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*PM RAACSSLEEQLPSGTGQLCSAPTAPTPSPGPPS PPPLAATPIFHLCPHHHTTQHTSRCRAPMG*VG SSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAPC TCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSGF P*WGLGGFPWP*KGPKPHLILARPTPLPWQQQV WPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP*S PY*VGQPPSVTGRQEG*VPYSLFTVATAPLPSA WDLSHLLSR*GPRALWPPPEVGWGCSPNPTLLP PLSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAAT SAKCSSSLPPVLPTIPSPKILFVGKRGWGMAGW VTDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKK NILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRSPAKCQHLRDLGADQREAKTRVLPRPGKPP QLQEGNQGADPKNWRRRKRRASRRSPRRRSSDP CVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHHH HRLWPPPPSSTCALTTTLHSTPAAAGLPWAEWG AVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PHP APAASPLPWWWGHCSLGFWFGGALSAPSLFPLAS HSGAWEGSPGLKRGPSPISSWHALLHCPGSSRC GQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFPS PRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPPP GI*VTYCPGEAQEPCGRHLRWAGAAPLTLLCFR HSAISPSSDGAPITRSSPCPLPTPLLLLPAPQG SGSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG *PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVG ATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK ISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESGAQRSANT*ETSGPTKGKQKQGCCQDPENHH NSRKETKGQTQKTGEGGRGGHLAGVLGGGAVTH ACRLLLTGGAASFWDWTALLRSHRPHPFPRPTI TTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSGE QFSPGLSSQLPPPTHAYTHALLDKANIPLSRTL HLLRPHSLGGGDIALWAFGLGAPSLLLHCSLWL PIVGPGRVPLALKGAQAPSHPGTPYSTALAAAGV ANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSLV PVLGWTAPFGYRKAGGVSPLLPLHCGHSPLALR LGSESLIVQVRPKSPVAAT*GGLGLLP*PYFASA TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKV LVPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCY QRQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*G PPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*K KYPFLDW

Sequence HMGA1.tAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA GGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCA CCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACC CATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCA TCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGG GGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACC CTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGC TTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGG TGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTA GTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCC GCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGG CTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCC CCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATT CTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGG GAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGG GCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGT TTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKT TTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*P MRAACSSLEEQLPSGTGQLCSAPTAPTPSPGPP SPPPLAATPIFHLCPHHHTTQHTSRCRAPMG*V GSSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAP CTCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSG FP*WGLGGFPWP*KGPKPHLILARPTPLPWQQQ VWPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP* SPY*VGQPPSVTGRQEG*VPYSLFTVATAPLPS AWDLSTYCGDGDAVTYCPGEAQEPCGRHLRWAG AAPLTLLCFRHSAISPSSDGAPITRSSPCPLPTP LLLLPAPQGSGSIFPLFTNYLWTVVLFFVQCSI LRHPSLLLLPAPNVHPHCLLFCPRSPPPRYSLW GRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*ARG PKSTIKGVGATSSPCSVGEG*P*FVPAWGSLSG FLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRRSPAKCQHLRDLGADQREAKTRVLPRPGKP PQLQEGNQGADPKNWRRRKRRASRRSPRRRSSD PCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHH HHRLWPPPPSSTCALTTTLHSTPAAAGLPWAEW GAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PH PAPAASPLPWWWGHCSLGFWFGGALSAPSLFPLA SHSGAWEGSPGLKRGPSPISSWHALLHCPGSSR CGQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFP SPRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPP PGI*VHIVVMEMQSLIVQVRPKSPVAAT*GGLG LLP*PYFASATQPFPPPQMGHQ*QGAHPARSQP PSCSSLPPKVLVPFFLCSQTTSGQLCCFLFNVPF FDIRHCCCYQRQMFILIASCSAHDPLPQDTLCG EEGLGHGRLGDRLPQSQGRWGPAPRMLQQSEQG GPNRP*RV*GPPPPPVLLGRGSHDLSQPGAPSLV SYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESEGAQRSANT*ETSGPTKGKQKQGCCQDPENH HNSRKETKGQTQKTGEGGRGGHLAGVLGGGAVT HACRLLLTGGAASFWDWTALLRSHRPHPFPRPT ITTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSG EQFSPGLSSQLPPPTHAYTHALLDKANIPLSRT LHLLRPHSLGGGDIALWAFGLGAPSLLLHCSLW LPIVGPGRVPLALKGAQAPSHPGTPYSTALAAAG VANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSL VPVLGWTAPFGYRKAGGVSPLLPLHCGHSPLAL RLGSEYILW*WRCSHLLSR*GPRALWPPPEVGWG CSPNPTLLPPLSHFPLLRWGTNNKELTLPAPNP PPAPPCPPRFWFHFSSVHKLPLDSCVVFCSMFH SSTSVIAAATSAKCSSSLPPVLPTIPSPKILFVG KRGWGMAGWVTDYPSPREGGALPLGCCSRVSKG AQIDHKGCRGHLLPLFCWGGVAMICPSLGLPLW FPICSYLNKKNILFWTG

Sequence HMGA1.qAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGA GGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCT TCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTC CCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATC CCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTC ACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGC CCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCT TCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACA GCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACC TGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTG CCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTT GTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAA GATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGC AGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGG GGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESSE EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP AKCQHLRDLGADQREAKTRVRRRKRRASRRSPR RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT *GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQG*EGGRGGHLAGVLG GGAVTHACRLLLTGGAASFWDWTALLRSHRPHP FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH SPLALRLGSEYILW*WRCSHLLSR*GPRALWPPP EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL GLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.oAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGA AGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAG CTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACA CTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATAC ACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTT TTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCC ATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTT TGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGA GTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAG GTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGA TGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACA AACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCC TCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAA GGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGG GAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQEPSEVPTPKRPRGRPKGSKNKGEKEE EEGISQESSEEEQ*PMRAACSSLEEQLPSGTGQ LCSAPTAPTPSPGPPSPPPLAATPIFHLCPHHH TTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTHT HMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGL LVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPKP HLILARPTPLPWQQQVWPMEGGAGPQDSPSQTVF VTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG* VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYCP GEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSSD GAPITRSSPCPLPTPLLLLPAPQGSGSIFPLFT NYLWTVVLFFVQCSILRHPSLLLLPAPNVHPHC LLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGK VGPCP*DAAAE*ARGPKSTIKGVGATSSPCSVG EG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRSPAKCQHLRDLGADQREAKTRVRRR KRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLDS FAPLPPPPPLPQAHHHHRLWPPPPSSTCALTTT LHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPRI HTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLGF WFGGALSAPSLFPLASHSGAWEGSPGLKRGPSP ISSWHALLHCPGSSRCGQWRGVLAPRIPPAKLS LSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGE SPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQ VRPKSPVAAT*GGLGLLP*PYFASATQPFPPPQ MGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQ TTSGQLCCFLFNVPFFDIRHCCCYQRQMFILIA SCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQG RWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLG RGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESGAQRSANT*ETSGPTKGKQKQG*EGG RGGHLAGVLGGGAVTHACRLLLTGGAASFWDWT ALLRSHRPHPFPRPTITTASGRHPHLPPVPSPPH YTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAY THALLDKANIPLSRTLHLLRPHSLGGGDIALWA FGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQAP SHPGTPYSTALAAAGVANGGGCWPPGFPQPNCL CHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGGV SPLLPLHCGHSPLALRLGSEYILW*WRCSHLLSR *GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLR WGTNNKELTLPAPNPPPAPPCPPRFWFHFSSVH KLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSLP PVLPTIPSPKILFVGKRGWGMAGWVTDYPSPRE GGALPLGCCSRVSKGAQIDHKGCRGHLLPLFCW GGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.mAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGA GGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGA CAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACC ACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCA TACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGG CTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGC CCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGT CTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGG TGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTC CAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTC AGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTC ACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATT GCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGG GAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTT GGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGEKE EEEGISQESSEEEQ*PMRAACSSLEEQLPSGTG QLCSAPTAPTPSPGPPSPPPLAATPIFHLCPHH HTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTH THMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSG LLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPK PHLILARPTPLPWQQQVWPMEGGAGPQDSPSQTV FVTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG *VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYC PGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSS DGAPITRSSPCPLPTPLLLLPAPQGSGSIFPLF TNYLWTVVLFFVQCSILRHPSLLLLPAPNVHPH CLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPG KVGPCP*DAAAE*ARGPKSTIKGVGATSSPCSV GEG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRRSPAKCQHLRDLGADQREAKTRVRR RKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLD SFAPLPPPPPLPQAHHHHRLWPPPPSSTCALTT TLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPR IHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLG FWFGGALSAPSLFPLASHSGAWEGSPGLKRGPS PISSWHALLHCPGSSRCGQWRGVLAPRIPPAKL SLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRG ESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIV QVRPKSPVAAT*GGLGLLP*PYFASATQPFPPP QMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCS QTTSGQLCCFLFNVPFFDIRHCCCYQRQMFILI ASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQ GRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLL GRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESEGAQRSANT*ETSGPTKGKQKQG*EG GRGGHLAGVLGGGAVTHACRLLLTGGAASFWDW TALLRSHRPHPFPRPTITTASGRHPHLPPVPSPP HYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHA YTHALLDKANIPLSRTLHLLRPHSLGGGDIALW AFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQA PSHPGTPYSTALAAAGVANGGGCWPPGFPQPNC LCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGG VSPLLPLHCGHSPLALRLGSEYILW*WRCSHLLS R*GPRALWPPPEVGWGCSPNPTLLPPLSHFPLL RWGTNNKELTLPAPNPPPAPPCPPRFWFHFSSV HKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSL PPVLPTIPSPKILFVGKRGWGMAGWVTDYPSPR EGGALPLGCCSRVSKGAQIDHKGCRGHLLPLFC WGGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.lAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCG AAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACC AAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCA CTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCA CCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTC AGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACT CCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAG GGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGC TGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAG CCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATT GTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTG CTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCC CAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGC TGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCA GGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGT GTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAA TAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPEPS EVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKP RGRPKKLEKEEEEGISQESSEEEQ*PMRAACSS LEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLAA TPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLAS VPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVPT PLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGLG GFPWP*KGPKPHLILARPTPLPWQQQVWPMEGGA GPQDSPSQTVFVTTWGSLFILPQLP*SPY*VGQ PPSVTGRQEG*VPYSLFTVATAPLPSAWDLSTY CGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLLC FRHSAISPSSDGAPITRSSPCPLPTPLLLLPAP QGSGSIFPLFTNYLWTVVLFFVQCSILRHPSLL LLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAWQ AG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKG VGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT* IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRSPA KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGN QGADPKNWRRRKRRASRRSPRRRSSDPCVPPAPH WRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWPP PPSSTCALTTTLHSTPAAAGLPWAEWGAVFPWP QFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASPL PWWWGHCSLGFWFGGALSAPSLFPLASHSGAWE GSPGLKRGPSPISSWHALLHCPGSSRCGQWRGV LAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLDS PLRLQEGRRGESPTPSSLWPQPPCPPPGI*VHI VVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PYF ASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLPP KVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHCC CYQRQMFILIASCSAHDPLPQDTLCGEEGLGHG RLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV *GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE *KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGAQR SANT*ETSGPTKGKQKQGCCQDPENHHNSRKET KGQTQKTGEGGRGGHLAGVLGGGAVTHACRLLL TGGAASFWDWTALLRSHRPHPFPRPTITTASGRH PHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPGL SSQLPPPTHAYTHALLDKANIPLSRTLHLLRPH SLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPGR VPLALKGAQAPSHPGTPYSTALAAAGVANGGGC WPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGWT APFGYRKAGGVSPLLPLHCGHSPLALRLGSEYIL W*WRCSHLLSR*GPRALWPPPEVGWGCSPNPTL LPPLSHFPLLRWGTNNKELTLPAPNPPPAPPCP PRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIAA ATSAKCSSSLPPVLPTIPSPKILFVGKRGWGMA GWVTDYPSPREGGALPLGCCSRVSKGAQIDHKG CRGHLLPLFCWGGVAMICPSLGLPLWFPICSYLN KKNILFWTG

Sequence HMGA1.jAug05

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS SLEEQLPSGTGQLCSAPTAPLPSAWDLSTYCGD GDAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGS GSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG* PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGA TSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK ISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP HWRSSFLLGLDSFAPLPQPPCPPPGI*VHIVVM EMQSLIVQVRPKSPVAAT*GGLGLLP*PYFASA TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQ RQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KK YPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL LTGGAASFWDWTALLRSHSPLALRLGSEYILW*W RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPP LSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWV TDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN ILFWTG

Sequence HMGA1.iAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA ACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCG CCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGC CTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCC ACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGG GAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGG TGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGA CAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACAT ATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACT TTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCC CCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGC TGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATG GCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAG GGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTT GAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS SLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLA SVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVP TPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEGG AGPQDSPSQTVFVTTWGSLFILPQLP*SPY*VG QPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLL CFRHSAISPSSDGAPITRSSPCPLPTPLLLLPA PQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAW QAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIK GVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT *IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP HWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWP PPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASP LPWWWGHCSLGFWFGGALSAPSLFPLASHSGAW EGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLD SPLRLQEGRRGESPTPSSLWPQPPCPPPGI*VH IVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLP PKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHC CCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*R V*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLL E*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL LTGGAASFWDWTALLRSHRPHPFPRPTITTASGR HPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPG LSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPG RVPLALKGAQAPSHPGTPYSTALAAAGVANGGG CWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEYI LW*WRCSHLLSR*GPRALWPPPEVGWGCSPNPT LLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIA AATSAKCSSSLPPVLPTIPSPKILFVGKRGWGM AGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSYL NKKNILFWTG

Sequence HMGA1.gAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGAC CCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAG GAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCC CAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCAT GGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCAC TTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTG TTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTG GCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCC CAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCC CCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAG GTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCG CTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTC AATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATA CTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAG TGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCT CCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQEPSEVPTPKRPRGRPKGSKNKGAAKT RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE *ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG SLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRSPAKCQHLRDLGADQREAKTRVLPR PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT *PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT* GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA PSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESGAQRSANT*ETSGPTKGKQKQGCCQD PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG GAVTHACRLLLTGGAASFWDWTALLRSHRPHPFP RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL PLWFPICSYLNKKNILFWTG

Sequence HMGA1.fAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAA GACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAG GAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTT CCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCC CATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCC CACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCA CTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCC CTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTT CCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAG CCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCT GAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGC CCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTG TTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAG ATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCA GAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGG GCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSE EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRRSPAKCQHLRDLGADQREAKTRVLP RPGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT *GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQ DPENHHNSRKETKGQTQKTGEGGRGGHLAGVLG GGAVTHACRLLLTGGAASFWDWTALLRSHRPHPF PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG LPLWFPICSYLNKKNILFWTG

Sequence HMGA1.bAug05

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Single exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQEPSEVPTPKRPRGRPKGSKNKGAAKT RKTTTTPGRKPRGRPKKLALLDLLGHQILQKSR EGKVPGLGCAHEK*GVPGINQTTSPCPALP*LC VGVPPSPAPRILRTSRGDLGSHLACVPLN*R*GK EAIGGRFVLPMSLCRRETAKELGPGRGRCWSSD VTHHTALEGTIQFWVPKQLVERLGGLSQEAASR GPLQLGS

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRSPAKCQHLRDLGADQREAKTRVLPR PGKPPQLQEGNQGADPKNWLSLTCWDIRFYRSPE RGRYLAWAVPMRSEGSQV*IRPHPPALPCPSCV WGSLPLLLLEYSELLGEILEVI*PVSPSIRDEE RKP*GEGLSFL*ASAEEKQRRSWALGGVGAGVLM *PTTLHWRAPSNSGSPNSWWKGSVG*VKKLPLG GHCS*GH

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESGAQRSANT*ETSGPTKGKQKQGCCQD PENHHNSRKETKGQTQKTGSP*PAGTSDFTEVQ RGEGTWPGLCP*EVRGPRYKSDHIPLPCPALVVC GGPSLSCS*NTQNF*GRSWKSSSLCPPQLEMRK GSHRGKVCPSYEPLQKRNSEGAGPWEGSVLEF* CDPPHCTGGHHPILGPQTAGGKARWAESRSCL*G ATAVRVT